CN115634183A - Perilla leaf extract and preparation method thereof - Google Patents
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Abstract
The invention provides a perilla leaf extract and a preparation method thereof, belonging to the technical field of plant extraction. The preparation method of the perilla leaf extract comprises the following steps: (1) Drying and crushing perilla leaves to obtain dry perilla leaf powder; (2) Mixing the dry perilla leaf powder with an ethanol water solution, and leaching to obtain a leaching solution; (3) Concentrating and drying the leaching liquor to obtain a crude extract; (4) Preparing the crude extract into a crude extract water solution, separating and purifying to obtain a refined extract, and concentrating and drying the refined extract to obtain the perilla leaf extract. The preparation method is safer, the steps are simple, the prepared perilla leaf extract contains various effective components, the inhibition rate of the extract on tyrosinase can reach more than 90%, and the application prospect is wider.
Description
Technical Field
The invention relates to the technical field of plant extraction, and particularly relates to a perilla leaf extract and a preparation method thereof.
Background
Tyrosinase is a key enzyme for in vivo melanin synthesis, and when tyrosinase in cells is accumulated excessively or the activity of the tyrosinase is too high, melanin is accumulated, and freckles, sunburn, chloasma, senile plaques and the like are formed. Most whitening and freckle-removing products in the current market take tyrosinase as an action target, and the generation of skin melanin is reduced by inhibiting the activity of the tyrosinase, so that the whitening effect is achieved. Novel tyrosinase inhibitors from natural sources that are low in toxicity and highly effective are preferred. The compounds such as flavone, flavonoid, anthocyanin and vitamin are mainly distributed in a plurality of natural plants, have various pharmacological actions and can effectively inhibit the activity of tyrosinase, and are generally concerned.
Perilla (Perilla frutescens (L.) Britt), also called Perilla frutescens and Perilla frutescens, are annual herbaceous dicotyledonous plants, have effects of inducing sweat, relieving exterior syndrome, promoting qi circulation, etc., and can be used for treating wind-cold type common cold, cough with excessive phlegm, stagnation of qi in spleen and stomach, chest distress and emesis; it can also be used for fish and soft-shelled turtle intoxication, abdominal pain, vomiting and diarrhea or rubella itching. Perilla frutescens is also a precious multi-purpose plant, the aerial parts of which include perilla leaf, perilla seed, perilla stem, purple perilla fruit, etc., all of which can be used as drugs, and perillaldehyde, alpha-linolenic acid, perilla oil, etc., extracted from perilla frutescens, are common raw materials in the industries of food, medicine and cosmetics. The perilla leaf is rich in multiple active ingredients such as polyphenol, flavone, anthocyanin, perillaldehyde, amino acid, polysaccharide and the like, and researches show that the perilla leaf extract has multiple physiological functions such as oxidation resistance, inflammation resistance, bacteria resistance, tumor resistance and the like. The perilla leaf extract can effectively inhibit the activity of various oxidases including P450, cyclooxygenase, xanthine oxidase and the like, but the research on the tyrosinase inhibition effect of the perilla leaf extract is very little at present, and the extraction rate of effective components with the tyrosinase inhibition effect in the perilla leaf is not high, so that the waste of perilla resources is caused.
Disclosure of Invention
The invention aims to provide a perilla leaf extract and a preparation method thereof. The preparation method effectively reserves the effective components in the perilla leaves, so that the perilla leaf extract has multiple types of active components, high content and high tyrosinase inhibitory activity, wherein the semi-inhibitory concentration of the extracts of the green leaf perilla and the purple leaf perilla on the tyrosinase activity is 9.84 +/-0.35 mg/ml and 9.69 +/-0.35 mg/ml respectively, and the inhibitory rate of the extracts of 20.0mg/ml on the tyrosinase can reach more than 90 percent. The preparation method is safer, simple in steps and wide in application prospect.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of a perilla leaf extract, which comprises the following steps:
(1) Drying and crushing perilla leaves to obtain dry perilla leaf powder;
(2) Mixing the dry perilla leaf powder with an ethanol water solution, and leaching to obtain a leaching solution;
(3) Concentrating and drying the leaching liquor to obtain a crude extract;
(4) Preparing the crude extract into a crude extract water solution, separating and purifying to obtain a refined extract, and concentrating and drying the refined extract to obtain the perilla leaf extract.
Preferably, the drying temperature is 50-60 ℃, the drying time is 3-5 h, and the grain size of the perilla leaf dry powder is 60-100 meshes.
Preferably, the volume fraction of the ethanol aqueous solution is 50-70%, and the mass volume ratio of the perilla leaf dry powder to the ethanol aqueous solution is 1g:10 to 20ml; the leaching is carried out at the temperature of 40-70 ℃ by stirring, the leaching time is 40-80 min, and the stirring speed is 100-500 rpm.
Preferably, the concentration of the aqueous solution of the crude extract is 0.5 to 1.5mg/ml.
Preferably, the separation and purification is to adopt AB-8 macroporous resin for adsorption and resolution; the mass-volume ratio of the AB-8 macroporous resin to the crude extract aqueous solution is 1g: 15-25 ml; the temperature during the adsorption is 20-30 ℃, and the adsorption time is 3-6 h.
Preferably, the analysis is carried out by using an ethanol water solution with the volume fraction of 50-70%, the temperature during the analysis is 20-30 ℃, and the time for the analysis is 4-6 h; the mass-to-volume ratio of the adsorbed macroporous resin to the ethanol aqueous solution during analysis is 1g:80 to 120ml; the adsorption and the desorption are carried out on a shaker, and the rotation speed of the shaker is independently 100-150 rpm.
Preferably, the concentration in the step (3) and the step (4) is reduced pressure rotary evaporation concentration, and the rotation speed of the reduced pressure rotary evaporation concentration is 100-200 rpm independently; the temperature of the rotary evaporation is independently 50-70 ℃; the pressure of rotary evaporation is independently 0.080-0.095 MPa;
the drying in the step (1) and the step (2) is freeze drying, the temperature of the freeze drying is independently-100 to-60 ℃, the pressure is independently less than 30Pa, and the time is independently 48 to 72 hours.
Preferably, the concentration in step (3) is to concentrate the leaching solution to 1/4-1/2 of the volume of the original leaching solution; and (4) concentrating the refined extract to 1/4-1/2 of the volume of the original refined extract.
The invention also provides a perilla leaf extract prepared by the preparation method, wherein the effective components of the perilla leaf extract comprise nicotinamide, apigenin-6, 8-di-C-glucoside, oleanolic acid, quercetin 3-beta-D-glucoside, apigenin-5-O-glucoside, luteolin-4' -O-glucoside, apigenin-7-O-beta-D-glucuronide, ursolic acid, scutellarin, salvianic acid, luteolin, apigenin and luteolin-7-O-beta-D-glucuronide.
The invention provides a perilla leaf extract and a preparation method thereof. The perilla leaf extract prepared by the method comprises a plurality of effective components such as nicotinamide, apigenin-6, 8-di-C-glucoside, oleanolic acid, quercetin 3-beta-D-glucoside, apigenin-5-O-glucoside, luteolin-4' -O-glucoside, apigenin-7-O-beta-D-glucuronide, ursolic acid, scutellarin, danshensu, luteolin, apigenin and luteolin-7-O-beta-D-glucuronide, effectively retains a plurality of active components, improves the content of the active components, and ensures that the obtained perilla leaf extract has high inhibitory activity to tyrosinase, wherein the half inhibitory concentrations of the extracts of green perilla leaf and purple perilla leaf to tyrosinase activity are respectively 9.84 +/-0.35 mg/ml and 9.69 +/-0.35 mg/ml, and the inhibitory rate of the extract with the concentration of 20.0mg/ml to tyrosinase can reach more than 90%. In addition, the source of the reagent used in the invention is wide, compared with the prior art that petroleum ether, chloroform, ethyl acetate, n-butanol and the like are mostly adopted, the reagent is safer, the steps are simpler, and the obtained perilla leaf extract has wide application prospects in foods and daily chemical products.
Drawings
FIG. 1 is a total ion flow graph of green leaf perilla leaf extract prepared in example 1;
FIG. 2 is a total ion flow graph of the purple leaf perilla leaf extract prepared in example 1;
FIG. 3 shows tyrosinase inhibitory activity of the leaf extract of green leaf perilla prepared in example 1;
FIG. 4 shows tyrosinase inhibitory activity of the purple perilla leaf extract prepared in example 1.
Detailed Description
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Putting fresh green leaf perilla leaf into a 50 ℃ oven for drying for 3h. Taking out the dried perilla leaves, crushing into powder by using a multifunctional crusher, sieving by using a 60-mesh sieve to obtain dried perilla leaf powder, and storing by using a self-sealing bag for later use.
Accurately weighing 2.0g of folium Perillae dry powder, adding 30ml of 60% ethanol aqueous solution, heating in water bath at 60 deg.C, and extracting under stirring at 200rpm for 60min. After extraction, naturally cooling the solution to room temperature, centrifuging at 5000rpm for 20min, removing precipitate, and collecting the leaching solution. Concentrating the obtained leaching liquor by reduced pressure rotary steaming at rotation speed of 150rpm, rotary steaming temperature of 55 deg.C and rotary steaming pressure of 0.090MPa, concentrating the volume of the leaching liquor to 1/3 of the original volume, and freeze drying at-80 deg.C and 30Pa for 48 hr to obtain crude extract.
Dissolving the crude extract in water to obtain 1.0mg/ml aqueous solution, and separating and purifying by AB-8 macroporous resin adsorption and desorption. The specific operation is as follows:
soaking AB-8 macroporous resin in 2 times volume of anhydrous ethanol for 36h until the resin fully expands, removing the anhydrous ethanol, soaking the soaked resin in 2 times volume of 5% hydrochloric acid (volume fraction) for 6h, removing acid liquor, adding 2 times volume of 5% sodium hydroxide (mass fraction) solution, soaking for 6h, and removing alkali liquor. Then adding acid liquor and alkali liquor in sequence according to the above operation, and repeatedly soaking for 2 times. And finally, adding deionized water into the resin after the acid-base activation treatment to clean the resin until the pH of the washing water is 7.0.
Weighing 1.0000g of the above activated AB-8 macroporous resin in a 100ml conical flask, adding 20ml of the above crude extract aqueous solution, and placing in a shaker at a rotation speed of 120rpm at 25 deg.C for 4 hours to reach adsorption equilibrium. Removing the supernatant after the adsorption balance, adding 20ml of deionized water to wash the resin twice, then adding 100ml of ethanol aqueous solution with the volume fraction of 70%, and placing the mixture in a shaking table with the rotating speed of 120rpm for resolving for 4 hours at the temperature of 25 ℃ so as to balance the resin. Collecting the resolved supernatant to obtain the refined extract.
Concentrating the refined extract by reduced pressure rotary steaming at rotation speed of 150rpm, 55 deg.C and 0.090MPa to 1/3 of original volume, and lyophilizing at-80 deg.C and 30Pa for 48 hr to obtain folium Perillae extract.
Taking fresh purple leaf perilla leaves, and preparing the purple leaf perilla leaf extract according to the steps.
Example 2
Oven drying fresh green leaf of Perilla frutescens in 55 deg.C oven for 4 hr. Taking out dried folium Perillae, pulverizing into powder with multifunctional pulverizer, sieving with 80 mesh sieve to obtain folium Perillae dry powder, and storing with self-sealing bag for use.
Accurately weighing 2.0g of folium Perillae dry powder, adding 20ml of 60% ethanol water solution, water bathing at 40 deg.C, and extracting under stirring at 100rpm for 80min. After extraction, naturally cooling the solution to room temperature, centrifuging at 5000rpm for 20min, removing precipitate, and collecting the leaching solution. Concentrating the obtained leaching liquor by reduced pressure rotary steaming at rotation speed of 100rpm, rotary steaming temperature of 70 deg.C and rotary steaming pressure of 0.080MPa, concentrating the volume of the leaching liquor to 1/4 of the original volume, and freeze drying at-80 deg.C and 20Pa for 54 hr to obtain crude extract.
Dissolving the crude extract in water to obtain 1.5mg/ml aqueous solution, and separating and purifying by AB-8 macroporous resin adsorption and desorption. The specific operation is as follows:
soaking AB-8 macroporous resin in 2 times volume of anhydrous ethanol for 36h until the resin fully expands, removing the anhydrous ethanol, soaking the soaked resin in 2 times volume of 5% hydrochloric acid (volume fraction) for 6h, removing acid liquor, adding 2 times volume of 5% sodium hydroxide (mass fraction) solution, soaking for 6h, and removing alkali liquor. Then adding acid liquor and alkali liquor in sequence according to the above operation, and repeatedly soaking for 2 times. And finally, adding deionized water into the resin after the acid-base activation treatment to clean the resin until the pH of the washing water is 7.0.
Weighing 1.0000g of the above activated AB-8 macroporous resin in a 100ml conical flask, adding 15ml of the above crude extract aqueous solution, and adsorbing in a shaker at 100rpm for 5 hours at 30 deg.C to reach adsorption equilibrium. Removing the supernatant of the resin after the adsorption balance, adding 20ml of deionized water to wash the resin twice, then adding 80ml of ethanol aqueous solution with the volume fraction of 50%, and placing the mixture in a shaking table with the rotating speed of 100rpm for resolving for 5 hours at the temperature of 30 ℃ so as to balance the resin. Collecting the resolved supernatant to obtain the refined extract.
Concentrating refined extract by rotary steaming under reduced pressure at rotation speed of 100rpm, rotary steaming temperature of 70 deg.C and rotary steaming pressure of 0.080MPa to 1/4 of original volume, and freeze drying at-80 deg.C and 20Pa for 48 hr to obtain folium Perillae extract.
Taking fresh purple leaf perilla leaves, and preparing the purple leaf perilla leaf extract according to the steps.
Example 3
Oven drying fresh green leaf of Perilla frutescens in 60 deg.C oven for 5 hr. Taking out the dried perilla leaves, crushing into powder by a multifunctional crusher, sieving by a 100-mesh sieve to obtain dried perilla leaf powder, and storing by a self-sealing bag for later use.
Accurately weighing 2.0g of folium Perillae dry powder, adding 40ml of 70% ethanol water solution, water bathing at 70 deg.C, and extracting under stirring at 500rpm for 40min. After extraction, naturally cooling the solution to room temperature, centrifuging at 5000rpm for 20min, removing precipitate, and collecting the leaching solution. Concentrating the obtained leaching liquor by rotary evaporation under reduced pressure at the rotation speed of 200rpm, the rotary evaporation temperature of 50 ℃ and the rotary evaporation pressure of 0.095MPa, concentrating the volume of the leaching liquor to 1/2 of the original volume, and freeze-drying at the temperature of-60 ℃ and the pressure of 30Pa for 72h to obtain a crude extract.
Dissolving the crude extract in water to obtain 1.0mg/ml aqueous solution, and separating and purifying by AB-8 macroporous resin adsorption and desorption. The specific operation is as follows:
soaking AB-8 macroporous resin in 2 times volume of anhydrous ethanol for 36h until the resin fully expands, removing the anhydrous ethanol, soaking the soaked resin in 2 times volume of 5% hydrochloric acid (volume fraction) for 6h, removing acid liquor, adding 2 times volume of 5% sodium hydroxide (mass fraction) solution, soaking for 6h, and removing alkali liquor. Then adding acid liquor and alkali liquor in sequence according to the above operation, and repeatedly soaking for 2 times. And finally, adding deionized water into the resin after the acid-base activation treatment to clean the resin until the pH of the washing water is 7.0.
Weighing 1.0000g of the above activated AB-8 macroporous resin in a 100ml conical flask, adding 25ml of the above crude extract aqueous solution, and adsorbing in a shaker at a rotation speed of 150rpm for 6 hours at 20 ℃ to reach adsorption balance. Removing the supernatant after the adsorption balance, adding 20ml of deionized water to wash the resin twice, then adding 120ml of ethanol aqueous solution with the volume fraction of 60%, and placing the mixture in a shaking table with the rotating speed of 150rpm at the temperature of 20 ℃ to analyze for 6 hours so as to balance the mixture. Collecting the resolved supernatant to obtain the refined extract.
Concentrating refined extract by rotary steaming under reduced pressure at rotation speed of 200rpm, 50 deg.C and 0.095MPa to 1/2 of original volume, and freeze drying at-60 deg.C and 30Pa for 72 hr to obtain folium Perillae extract.
Taking fresh purple leaf perilla leaves, and preparing the purple leaf perilla leaf extract according to the steps.
Test example 1
The green leaf perilla leaf extract and the purple leaf perilla leaf extract prepared in example 1 were subjected to component identification, specifically as follows:
10mg of each of the green leaf perilla leaf extract and the purple leaf perilla leaf extract prepared in example 1 was dissolved in 0.50ml of an 80% methanol aqueous solution. Standing in an ice bath for 5min, centrifuging for 20min at 15000g, taking supernatant, adding water to dilute the supernatant until the content of methanol is 53%, placing the supernatant in a 1.5 ml sample injection bottle, injecting 2ul of sample, and analyzing by lC-MS.
The instrument comprises the following steps: mass spectrometer
6500+ Mass Spectrometry platform, chromatograph is ExionilC from SCIEX, column is Waters (XSelectHSST 3,2.5 μm, 2.1X 150 mm), column temperature 50 ℃.
Mobile phase: mobile phase a was 0.1% formic acid-water solution and mobile phase B was 0.1% formic acid-acetonitrile solution.
Elution procedure: gradient elution (flow rate 0.4 ml/min): 0min:98% by weight A,2% by weight B;2min:98% by weight A,2% by weight B;15min:0% A,100% B; and (17 min): 0% A,100% B;17.1min:98% by weight A,2% by weight B;20min, 98% A,2% B.
Mass spectrum conditions: positive ion mode: air curtain gas (CurtainGas): 35psi; collisionGas (collision gas): medium; ion spray voltage: 5500V; temperature: 550 ℃; ion source gas 1:60psi; ion source gas 2:60psi; negative ion mode: air curtain gas (CurtainGas): 35psi; collisingGas: medium; ion spray pressure: -4500V; temperature: 550 ℃; ion source gas 1:60psi; ion source gas 2:60psi.
The total ion flow patterns of the green leaf perilla leaf extract and the purple leaf perilla leaf extract are shown in fig. 1 and fig. 2. The results of the component analysis are shown in Table 1.
TABLE 1 analysis table of liquid phase mass spectrometry components of green leaf perilla leaf extract and purple leaf perilla leaf extract
The qualitative analysis of the substances can be carried out according to the comparison database of the parent ions, the daughter ions, the retention time, the declustering voltage and the collision energy of each compound; the peak area of the ion integrated in each chromatographic retention time represents the relative content of the corresponding substance, and the relative content of each substance can be judged according to the peak area. As can be seen from Table 1, the perilla leaf extract prepared in the present invention contains nicotinamide (C) 6 H 6 N 2 O), apigenin-6, 8-di-C-glucoside (molecular formula C) 27 H 30 O 15 ) Oleanolic acid (C) 30 H 48 O 3 ) Quercetin 3-beta-D-glucoside (C) 21 H 20 O 12 ) apigenin-5-O-glucoside (C) 21 H 20 O 11 ) Luteolin-4' -O-glucoside (C) 21 H 20 O 11 ) apigenin-7-O-beta-D-glucuronide (C) 21 H 18 O 11 ) Ursolic acid (C) 30 H 48 O 3 ) Scutellarin (C) 21 H 18 O 12 ) Danshensu (C) 9 H 10 O 5 ) Luteolin (C) 15 H 10 O 6 ) Apigenin (C) 15 H 10 O 5 ) luteolin-7-O-beta-D-glucuronide (C) 21 H 18 O 12 ) And the like.
Test example 2
The inhibitory activity of the green leaf perilla leaf extract and the purple leaf perilla leaf extract prepared in example 1 on tyrosinase was examined, specifically as follows:
samples of green leaf perilla leaf extract and purple leaf perilla leaf extract prepared in example 1, which were prepared in a series of concentrations of 0.2, 1.0, 5.0, 10.0, 15.0 and 20.0mg/ml using phosphate buffered saline (PBS, 0.1mol/l, ph 6.8) as a solvent, were added to a 96-well plate using levodopa (1 mmol/l, prepared using PBS of 0.1mol/l, ph 6.8) as a substrate according to the reaction system shown in table 2, and the phosphate buffered saline (0.1 mol/l, ph 6.8), an enzyme solution (200U/ml) and the sample solution were incubated at 37 ℃ for 15min. Subsequently, levodopa, a substrate, was added and the temperature was maintained at 37 ℃ for 15min. Finally, the absorbance was measured at 475 nm. Tyrosinase inhibition was calculated according to formula (1).
Inhibition ratio (%) = [1- (T) 1 -T 0 )/(A 1 -A 0 )]X 100 formula (1)
In the formula: t is 1 Representing the absorbance of the sample containing tyrosinase; t is a unit of 0 Represents the absorbance of a sample control without tyrosinase; a. The 1 Represents the absorbance of a control without sample; a. The 0 Representative of the absorbance of a blank control containing no sample and tyrosinase
TABLE 2 reaction System
| Additive solution | A 0 (ul) | A 1 (ul) | T 0 (ul) | T 1 (ul) |
| |
40 | 20 | 20 | 0 |
| Sample(s) | 0 | 0 | 20 | 20 |
| |
0 | 20 | 0 | 20 |
| Substrate | 160 | 160 | 160 | 160 |
The results of the detection are shown in FIGS. 3 and 4.
As can be seen from FIGS. 3 and 4, the inhibitory activity of perilla leaf extract against tyrosinase increased with increasing concentration, and exhibited better linear relationship and dose effect relationship. The half inhibitory concentrations (IC 50) of the extracts of green leaf Perilla and purple leaf Perilla on tyrosinase activity were 9.84 + -0.35 mg/ml and 9.69 + -0.35 mg/ml, respectively. The perilla leaf extract has a good inhibition function on tyrosinase activity, can prevent or interrupt melanin formation, can be used as a potential tyrosinase inhibitor, and becomes a natural raw material of the whitening cosmetic.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.
Claims (9)
1. A preparation method of perilla leaf extract is characterized by comprising the following steps:
(1) Drying and crushing perilla leaves to obtain dry perilla leaf powder;
(2) Mixing the dry perilla leaf powder with an ethanol water solution, and leaching to obtain a leaching solution;
(3) Concentrating and drying the leaching liquor to obtain a crude extract;
(4) And preparing the crude extract into a crude extract water solution, separating and purifying to obtain a refined extract, and concentrating and drying the refined extract to obtain the perilla leaf extract.
2. The preparation method according to claim 1, wherein the drying temperature is 50-60 ℃, the drying time is 3-5 h, and the grain size of the dried perilla leaf powder is 60-100 meshes.
3. The method according to claim 2, wherein the volume fraction of the ethanol aqueous solution is 50 to 70%, and the mass-to-volume ratio of the perilla leaf dry powder to the ethanol aqueous solution is 1g:10 to 20ml; the leaching is carried out at the temperature of 40-70 ℃ by stirring, the leaching time is 40-80 min, and the stirring speed is 100-500 rpm.
4. The method of claim 3, wherein said aqueous crude extract solution has a concentration of 0.5 to 1.5mg/ml.
5. The preparation method according to claim 4, wherein the separation and purification is adsorption and desorption by using AB-8 macroporous resin; the mass-volume ratio of the AB-8 macroporous resin to the crude extract aqueous solution is 1g: 15-25 ml; the temperature during the adsorption is 20-30 ℃, and the adsorption time is 3-6 h.
6. The preparation method according to claim 5, wherein the analysis is performed by using 50-70% by volume of ethanol aqueous solution, the temperature during the analysis is 20-30 ℃, and the time for the analysis is 4-6 h; the mass-to-volume ratio of the adsorbed macroporous resin to the ethanol aqueous solution during analysis is 1g:80 to 120ml; the adsorption and the desorption are carried out on a shaker, and the rotation speed of the shaker is independently 100-150 rpm.
7. The method according to claim 6, wherein the concentration in the steps (3) and (4) is reduced pressure rotary evaporation concentration, and the rotation speed of the reduced pressure rotary evaporation concentration is 100-200 rpm independently; the temperature of rotary evaporation is independently 50-70 ℃; the pressure of rotary evaporation is independently 0.080-0.095 MPa;
the drying in the step (1) and the step (2) is freeze drying, the temperature of the freeze drying is independently-100 to-60 ℃, the pressure is independently less than 30Pa, and the time is independently 48 to 72 hours.
8. The process according to claim 7, wherein the concentration in step (3) is to concentrate the leachate to 1/4-1/2 of the volume of the original leachate; and (4) concentrating the refined extract to 1/4-1/2 of the volume of the original refined extract.
9. A perilla leaf extract prepared by the preparation method according to any one of claims 1 to 8, wherein the active ingredients of the perilla leaf extract include niacinamide, apigenin-6, 8-di-C-glucoside, oleanolic acid, quercetin 3-beta-D-glucoside, apigenin-5-O-glucoside, luteolin-4' -O-glucoside, apigenin-7-O-beta-D-glucuronide, ursolic acid, scutellarin, salvianic acid, luteolin, apiolin, and luteolin-7-O-beta-D-glucuronide.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07187989A (en) * | 1993-12-27 | 1995-07-25 | Nagase & Co Ltd | Extracted solution of perilla frutescens and skin-beautifying cosmetic containing the same |
| CN107440949A (en) * | 2016-05-30 | 2017-12-08 | 上海家化联合股份有限公司 | Composition and its application comprising extractive of perilla and Radix Arnebiae extract |
| CN110840930A (en) * | 2019-12-11 | 2020-02-28 | 中北大学 | Method for extracting xanthine oxidase inhibitor from perilla leaves with assistance of ultrasonic waves |
-
2022
- 2022-11-16 CN CN202211438493.8A patent/CN115634183A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07187989A (en) * | 1993-12-27 | 1995-07-25 | Nagase & Co Ltd | Extracted solution of perilla frutescens and skin-beautifying cosmetic containing the same |
| CN107440949A (en) * | 2016-05-30 | 2017-12-08 | 上海家化联合股份有限公司 | Composition and its application comprising extractive of perilla and Radix Arnebiae extract |
| CN110840930A (en) * | 2019-12-11 | 2020-02-28 | 中北大学 | Method for extracting xanthine oxidase inhibitor from perilla leaves with assistance of ultrasonic waves |
Non-Patent Citations (1)
| Title |
|---|
| 田景振、侯林: "抗病毒中草药的研究与应用", 山东科学技术出版社, pages: 299 - 301 * |
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