CN115627278A - Method for preparing lactic acid by continuous fermentation - Google Patents
Method for preparing lactic acid by continuous fermentation Download PDFInfo
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- CN115627278A CN115627278A CN202211442923.3A CN202211442923A CN115627278A CN 115627278 A CN115627278 A CN 115627278A CN 202211442923 A CN202211442923 A CN 202211442923A CN 115627278 A CN115627278 A CN 115627278A
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- fermentation
- lactic acid
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- clear liquid
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 86
- 238000000855 fermentation Methods 0.000 title claims abstract description 75
- 230000004151 fermentation Effects 0.000 title claims abstract description 75
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 43
- 239000004310 lactic acid Substances 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000001914 filtration Methods 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 238000011001 backwashing Methods 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims abstract description 14
- 239000000126 substance Substances 0.000 claims abstract description 10
- 239000008223 sterile water Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000005374 membrane filtration Methods 0.000 claims description 8
- 238000010992 reflux Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 238000001471 micro-filtration Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 12
- 235000000346 sugar Nutrition 0.000 description 8
- 230000008878 coupling Effects 0.000 description 7
- 238000010168 coupling process Methods 0.000 description 7
- 238000005859 coupling reaction Methods 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 239000000919 ceramic Substances 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 239000000178 monomer Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 241000186660 Lactobacillus Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- -1 corn Chemical class 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/147—Microfiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Water Supply & Treatment (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing lactic acid by continuous fermentation, which comprises the following steps: each independent fermentation system operates independently to produce lactic acid; the fermentation liquor in the independent fermentation system is filtered by a high-efficiency precise filtering system to obtain sterile clear liquid, and the sterile clear liquid is filtered and then directly enters a next-stage fermentation system for continuous fermentation; returning the obtained filtered turbid liquid to a fermentation system corresponding to the filtering system to continue to participate in fermentation; when the membrane component in the filtering equipment reaches a certain resistance value, process backwashing is carried out, a process backwashing medium is finished by adopting sterile water or tail end lactic acid clear liquid, and backwashing substances directly enter a fermentation system corresponding to the filtering system. The method has simple process steps, is easy to master, and can effectively improve the utilization rate of bacteria and achieve the aims of improving the yield of lactic acid and obtaining high-purity lactic acid.
Description
Technical Field
The invention relates to the technical field of lactic acid preparation, in particular to a method for preparing lactic acid by continuous fermentation.
Background
Lactic acid is an extremely important organic acid and is widely used in the food, pharmaceutical and chemical industries. The industrial production of lactic acid mainly adopts a microbial fermentation method, and most fermentation raw materials mainly comprise starch sugars such as corn, sweet potato and the like in China. The lactic acid fermentation broth has complex components, and contains various impurities such as thalli, residual sugar, protein, pigment, other organic acids, inorganic salts and the like besides lactic acid. As the fermentation conditions are suitable for bacterial reproduction, and the fermentation process is easy to generate mixed bacterial pollution, the whole fermentation fails, and huge economic loss is caused, so that the form of single fermentation of monomers is generally adopted. In single fermentation of the monomer, a mature and stable fermentation environment system needs to be built again for each fermentation, so that a large amount of basic substances are consumed, the fermentation benefit is low, and the time period is long.
Disclosure of Invention
The present invention aims at providing a method for preparing lactic acid by continuous fermentation, so as to solve the technical problems.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing lactic acid by continuous fermentation comprises the following steps:
step 1, each independent fermentation system operates independently to produce lactic acid.
2, filtering the fermentation liquor in the independent fermentation system by a high-efficiency precise filtering system to obtain sterile clear liquid, and directly feeding the filtered sterile clear liquid into a next-stage fermentation system for continuous fermentation;
As a further scheme of the invention, in the step 2, the high-efficiency precise filtering system adopts a fine filter with the filtering mesh number of more than 400 meshes (38 um), the pressure of the feeding and discharging liquid is not more than 1bar, and the automatic pollution discharge is realized when the pressure is more than 1 bar.
As a further scheme of the invention, the high-efficiency precise filtration system in the step 2 adopts a microfiltration membrane filter, the material temperature is not higher than 50 ℃, the feeding pressure is 5-8 bar, the membrane circulation pressure is controlled to be 3-5 bar, and the reflux ratio of membrane filtration is 2-3.
As a further scheme of the invention, when the membrane filtration pressure adopted in the step 2 is more than 5.5bar, the back washing system is automatically started.
Compared with the prior art, the invention has the following advantages: the invention intercepts the unusable impurities and recovers bacteria by combining the fine filtration and the membrane filtration, and the process steps are simple and easy to master; the loss rate in the whole process step is very low, the utilization rate of bacteria can be effectively improved, the accumulation of lactic acid in a fermentation system is reduced, the phenomenon of fermentation retardation caused by the increase of the content of the lactic acid in the system is relieved, and the aims of improving the yield of the lactic acid and obtaining the lactic acid with higher purity are finally achieved. The sewage discharge capacity can be reduced by 90 percent compared with the traditional method, and the environmental protection pressure is greatly reduced.
Drawings
FIG. 1 is a diagram of a system for preparing lactic acid by continuous fermentation according to the present invention.
In the figure: 1 independent fermentation system, 2 fine filter and 3 micro-filtration membrane filter.
Detailed Description
The invention is explained in further detail below with reference to the figures and the specific embodiments.
As shown in fig. 1, a method for preparing lactic acid by continuous fermentation comprises the following steps:
step 1, each independent fermentation system 1 operates independently to produce lactic acid.
In the step 2, the high-efficiency precise filtering system adopts the fine filter 2 to filter the materials with the number of meshes larger than 400 (38 um), the pressure of the feeding and discharging liquid is not larger than 1bar, and the automatic pollution discharge is realized when the pressure is larger than 1 bar. The high-efficiency precise filtration system in the step 2 adopts a microfiltration membrane filter 3, the material temperature is not higher than 50 ℃, the feeding pressure is 5-8 bar, the membrane circulation pressure is controlled to be 3-5 bar, the membrane filtration reflux ratio is 2-3, and when the membrane filtration pressure in the step 2 is more than 5.5bar, the backwashing system is automatically started.
The retention rates for the fine filter and micro (ultra) filter membrane filtration are given in the following table:
| species of matter | Fine filter 38um | Micro (ultra) filter membrane 0.1um |
| Coarse and colloidal precipitates | √ | |
| Fine and colloidal precipitate | √ | √ |
| Macromolecular organic substances such as protein | √ | |
| Bacteria | √ |
The fine filter can intercept SS in the fermentation liquor and insoluble substances (bacteria cannot be utilized) visible to the naked eyes, and the fine filter is used as a front protective filter for regularly discharging slag. The micro (ultra) filter membrane (hollow membrane or ceramic filtration with the same precision) has strong interception and prevention effects on bacteria, and can independently intercept the bacteria to flow back to the original system to continue to participate in fermentation.
Example 1
(1) Respectively inoculating lactobacillus in three fermentation tanks of three-stage coupling continuous fermentation according to the process requirement, starting to run in series coupling after 48 hours, and adding nutrient substances and carbon sources in a flowing manner according to the process requirement.
(2) The filtration reflux ratio of each membrane was set to 3 by using a 0.1um ceramic membrane.
(3) Detecting the content of lactic acid and carbon source in three fermentation tanks:
1# tank: 22g/L of lactic acid and 28g/L of reducing sugar;
2# tank: 37g/L of lactic acid and 9g/L of reducing sugar;
3# Can: 45g/L of lactic acid and 0g/L of reducing sugar.
(4) Detecting the number of bacteria in coupling fermentation: (each microscopic observation)
18000-23000 # tanks 1;
18000-20000 # tanks;
20000-250000 tanks 3.
(5) The final lactic acid content of the monomer in one single fermentation is 32 g/L, the maximum bacteria number is equivalent to that of a 3# tank, and the fermentation yield is improved by 40%.
Example 2
(1) Lactic acid bacteria are respectively inoculated in three fermentation tanks of three-stage coupling continuous fermentation according to the process requirement, the series coupling operation is started after 32 hours, and nutrient substances and carbon sources are added in a flowing manner according to the process requirement.
(2) And (3) changing a pH regulator: the original calcium preparation is adjusted to be sodium preparation.
(3) A 0.1um ceramic membrane was used, and the reflux ratio was set to 3.
(4) The lactic acid and carbon source contents in three fermentors were measured:
1# tank: 18g/L of lactic acid and 25g/L of reducing sugar;
2# tank: 25g/L of lactic acid and 10g/L of reducing sugar;
3# tank: 30g/L of lactic acid and 0g/L of reducing sugar.
(5) Detecting the number of bacteria in coupled fermentation: the reduction was compared to the previous experiment.
(6) The final lactic acid content is 24 g/L by single-stage fermentation at the same time, and the fermentation yield is improved by 25 percent.
Example 3
(1) Respectively inoculating lactobacillus in three fermentation tanks for three-stage coupling continuous fermentation according to process requirements, starting series coupling operation after 48 hours, and adding nutrients and carbon sources in a flowing manner according to the process requirements.
(2) A0.1 um hollow micro-membrane is adopted, and the filtration reflux ratio of each membrane is set to be 3.
(3) The content of lactic acid and carbon source and the number of bacteria in three fermentation tanks are detected, and the fermentation yield is improved by 35 percent compared with the case 1.
The foregoing is a preferred embodiment of the present invention, and it will be apparent to those skilled in the art that variations, modifications, substitutions and alterations can be made in the embodiment without departing from the principles and spirit of the invention.
Claims (4)
1. A method for preparing lactic acid by continuous fermentation is characterized by comprising the following steps:
step 1, each independent fermentation system operates independently to generate lactic acid;
step 2, filtering the fermentation liquor in the independent fermentation system by a high-efficiency precise filtering system to obtain sterile clear liquid, and directly feeding the filtered sterile clear liquid into a next-stage fermentation system for continuous fermentation;
step 3, returning the filtered turbid liquid obtained in the step 2 to a fermentation system corresponding to the filtering system, and continuing to participate in fermentation; when the membrane component in the filtering equipment reaches a certain resistance value, the process backwashing is carried out, the process backwashing medium is finished by adopting sterile water or tail end lactic acid clear liquid, and backwashing substances directly enter a fermentation system corresponding to the filtering system.
2. The method for preparing lactic acid by continuous fermentation according to claim 1, wherein the high-efficiency precise filtering system in step 2 adopts a fine filter with the filtering mesh number of more than 400 meshes (38 um), the pressure of the feed and discharge liquid is not more than 1bar, and automatic pollution discharge is realized when the pressure is more than 1 bar.
3. The method for preparing lactic acid by continuous fermentation as claimed in claim 2, wherein the material temperature of the high-efficiency precise filtration system of step 2 using a microfiltration membrane filter is not higher than 50 ℃, the feeding pressure is 5-8 bar, the circulation pressure of the membrane is controlled to be 3-5 bar, and the reflux ratio of the membrane filtration is 2.
4. The method for preparing lactic acid by continuous fermentation according to claim 3, wherein the backwashing system is automatically started when the membrane filtration pressure in step 2 is greater than 5.5 bar.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211442923.3A CN115627278A (en) | 2022-11-18 | 2022-11-18 | Method for preparing lactic acid by continuous fermentation |
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| CN202211442923.3A CN115627278A (en) | 2022-11-18 | 2022-11-18 | Method for preparing lactic acid by continuous fermentation |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6596521B1 (en) * | 1999-04-13 | 2003-07-22 | Korea Advanced Institute Of Science And Technology | Method for manufacturing organic acid by high-efficiency continuous fermentation |
| CN101392273A (en) * | 2008-11-10 | 2009-03-25 | 南京工业大学 | Clean production process of lactic acid |
| CN103725605A (en) * | 2013-12-31 | 2014-04-16 | 陕西万源生物农业科技有限公司 | Fermentation tank and fermentation system |
| CN103834696A (en) * | 2013-10-23 | 2014-06-04 | 中国科学院过程工程研究所 | Method for producing lactic acid through continuously fermenting batches of lignocellulose hydrolysate by coupling fermenting and membrane separation |
| CN106755143A (en) * | 2017-01-11 | 2017-05-31 | 南京工业大学 | Method for continuously extracting high-purity lactic acid from lactic acid fermentation liquor |
| CN108410700A (en) * | 2018-04-27 | 2018-08-17 | 山东康得生物科技有限公司 | A kind of production of ferment is continuously fermented system and process |
-
2022
- 2022-11-18 CN CN202211442923.3A patent/CN115627278A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6596521B1 (en) * | 1999-04-13 | 2003-07-22 | Korea Advanced Institute Of Science And Technology | Method for manufacturing organic acid by high-efficiency continuous fermentation |
| CN101392273A (en) * | 2008-11-10 | 2009-03-25 | 南京工业大学 | Clean production process of lactic acid |
| CN103834696A (en) * | 2013-10-23 | 2014-06-04 | 中国科学院过程工程研究所 | Method for producing lactic acid through continuously fermenting batches of lignocellulose hydrolysate by coupling fermenting and membrane separation |
| CN103725605A (en) * | 2013-12-31 | 2014-04-16 | 陕西万源生物农业科技有限公司 | Fermentation tank and fermentation system |
| CN106755143A (en) * | 2017-01-11 | 2017-05-31 | 南京工业大学 | Method for continuously extracting high-purity lactic acid from lactic acid fermentation liquor |
| CN108410700A (en) * | 2018-04-27 | 2018-08-17 | 山东康得生物科技有限公司 | A kind of production of ferment is continuously fermented system and process |
Non-Patent Citations (1)
| Title |
|---|
| 于培星: "连续循环发酵生产L-乳酸新技术研究", 中国食品添加剂, no. 5, 15 October 2010 (2010-10-15), pages 124 - 128 * |
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