CN115626871B - Curcumin, extraction method thereof and natural anti-inflammatory composition - Google Patents
Curcumin, extraction method thereof and natural anti-inflammatory composition Download PDFInfo
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- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
The invention relates to the technical field of foods, in particular to curcumin, an extraction method thereof and a natural anti-inflammatory composition. The extraction method of curcumin comprises the following steps: adding 10-15 times of water into turmeric powder which is dried by hot air until the content is less than 10%, decocting for 10-20 minutes, adding neutral protease for enzymolysis for 10-15 minutes, and filtering to obtain enzymolysis liquid; sequentially adding starch and soybean oil into the enzymolysis solution for adsorption, and centrifuging to obtain precipitate; adding amylase into the precipitate for enzymolysis for 1-3 min, extracting with ethanol, removing ethanol to obtain an extracting solution, adding soybean protein isolate with the mass of 1% -3% of that of turmeric, heating at 100 ℃ for 3-6 min, and drying until the water content is less than 10% to obtain turmeric powder. The extraction method of the invention is simple, low in cost and high in extraction rate, and has good antioxidant and anti-inflammatory effects with the composition of Jiang Man raspberry powder and the like.
Description
Technical Field
The invention relates to the technical field of foods, in particular to curcumin, an extraction method thereof and a natural anti-inflammatory composition.
Background
Curcumin (Curcumin) is a yellow pigment extracted from rhizome of Curcuma longa of Zingiberaceae. The main chain of the acidic polyphenols is unsaturated aliphatic and aromatic groups. Is commonly used as meat food coloring agent and acid-base indicator, and has anti-inflammatory and antioxidant pharmacological effects.
The existing curcumin extraction method is complex, requires purification steps and the like, and has high cost such as manpower, time and the like.
Disclosure of Invention
In view of the above-mentioned drawbacks and disadvantages of the prior art, the present invention provides a method for extracting curcumin, which combines the technical means of hot air drying, protease enzymolysis and starch adsorption in sequence to increase the extraction rate of curcumin;
correspondingly, the invention also provides curcumin;
correspondingly, the invention also provides application of curcumin in a natural anti-inflammatory composition.
In order to achieve the above purpose, the main technical scheme adopted by the invention comprises the following steps:
the invention provides a curcumin extraction method, which comprises the following steps:
s1, adding 10-15 times of water into turmeric powder which is dried by hot air until the content is less than 10%, decocting for 10-20 minutes, adding neutral protease for enzymolysis for 10-15 minutes, and filtering to obtain enzymolysis liquid;
s2, sequentially adding starch and soybean oil into the enzymolysis liquid for adsorption, and centrifuging to obtain a precipitate;
and (3) adding amylase into the precipitate for enzymolysis for 1-3 min, extracting with ethanol, removing ethanol to obtain an extracting solution, adding soybean protein isolate with the mass of 1% -3% of that of turmeric, heating at 100 ℃ for 3-6 min, and drying until the moisture content is less than 10% to obtain turmeric powder.
According to the invention, most volatile substances are taken away by volatilization of hot air drying water and air, so that the influence of the volatile substances on curcumin extraction is avoided, preferably, the temperature of hot air drying is controlled to be 70-80 ℃, and the time of hot air drying is controlled to be 20-30 min. The microstructure of the turmeric is destroyed by decoction, so that more curcumin is exposed; the neutral protease is used for enzymolysis, so that the protein is degraded to further promote the free curcumin, and the subsequent adsorption of the starch on the curcumin is facilitated; although starch has limitation on the adsorption capacity of curcumin, the adsorption of starch and soybean oil can improve the extraction rate of curcumin. The adding sequence of the starch and the soybean oil is necessary, the soybean oil adsorbed with the curcumin is centrifugated to form a precipitate with the starch, the precipitate is subjected to enzymolysis by amylase, the starch is degraded into glucose, the curcumin and the soybean oil can be extracted by ethanol alcohol extraction, the curcumin is coated to form small particles under the interaction of the soybean protein isolate and the soybean oil, the storage stability of the small particles is improved, and finally the turmeric powder with strong anti-inflammatory effect is obtained by drying.
In the optional scheme of the curcumin extraction method, the addition amount of neutral protease is 3200-3500U/g turmeric powder, and the enzymolysis temperature is 40-45 ℃.
According to the curcumin extraction method, in an optional scheme, the addition amount of starch is 3% -5% of the weight of turmeric powder, and the addition amount of amylase is 1300-1600U/g of the weight of starch.
In a second aspect, the present invention also provides turmeric powder prepared by the method for extracting curcumin described in any one of the above schemes.
In a third aspect, the invention also provides a natural anti-inflammatory composition, which comprises 2-5 parts by weight of turmeric powder, 4-6 parts by weight of cranberry powder, 2-5 parts by weight of licorice powder and 4-8 parts by weight of astragalus powder obtained in any one of the above schemes.
Optionally, the preparation of cranberry powder includes the steps of:
squeezing fresh cranberry to obtain juice and residue;
the method comprises the steps of performing enzymolysis on cellulase and protease of cranberry residues according to a mixed complex enzyme with an enzyme activity ratio of 1:1-2, and separating to obtain an enzymolysis solution and enzymolysis residues, wherein the addition amount of the complex enzyme is 100-250U/g of the weight of fresh cranberry, the enzymolysis temperature is 28-32 ℃, and the enzymolysis time is 2-10 min;
ultrafiltration treatment of cranberry juice, separating fine slag and juice;
ethanol extraction of the fine slag to obtain an ethanol extract, ethanol removal treatment and water addition to prepare a suspension;
homogenizing the suspension and juice, mixing with enzymolysis solution, and drying to obtain cranberry powder.
Optionally, the ultrafiltration is carried out for 1-3 min after 3000 r/min-3500 r/min; the pore size of the ultrafiltration membrane is 50-100 nm.
Alternatively, the protease is a subtilisin.
Optionally, the concentration of the ethanol is 40-50%, and the addition amount of the ethanol is 1-1.5 times of the weight of the fresh cranberry; the ratio of the alcohol extract to the water is 1:5-10.
The invention improves the solubility of the cranberry powder by a degradation method of cellulase combined with protease, and widens the application range of the cranberry powder; wherein, the enzyme complex of cellulase and protease can improve the solubility of the cranberry powder in various solution systems for the enzymolysis product of the cranberry residue and the alcohol extract of the fine residue.
The ultrafiltration treatment device comprises a central sphere, ultrafiltration mechanisms arranged on the left side and the right side of the central sphere and filter membrane regeneration mechanisms arranged on the upper side and the lower side of the central sphere; the center sphere comprises a spherical shell and four arc panels arranged in the spherical shell; the four cambered surface plates are spliced and enclosed to form a spherical inner cavity; a discharge electrode is arranged in the middle of the spherical inner cavity; the outer surfaces of the left cambered surface plate and the right cambered surface plate are fixedly connected with an ultrafiltration piston rod respectively; the outer surfaces of the upper cambered surface plate and the lower cambered surface plate are fixedly connected with a plug rod for life again and again respectively;
the ultrafiltration mechanism and the regeneration mechanism respectively comprise rectangular first liquid cavities which are lengthened in the left-right direction and lengthened in the up-down direction; one end of the first liquid cavity is connected with the spherical shell, and the other end of the first liquid cavity is provided with a net body roller; the net body roller comprises a first rotating shaft positioned at the center and a hollow cylindrical net body sleeved outside the first rotating shaft at intervals; the first rotating shaft is connected with the hollow cylindrical net body through a plurality of connecting rods; one end of the two opposite first wall plates of the first liquid cavity is lapped against the surface of the net body; the overlapping part of the first wall plate and the surface of the net body is wedge-shaped; a guide roller is respectively arranged at the included angle between two adjacent first liquid cavities; an ultrafiltration membrane body is sequentially tensioned along the surface of the net body and the guide roller, and the whole ultrafiltration membrane body is tensioned into a cross shape; the first wall plate and the net body are respectively provided with a squeeze roller; the diameter of the extrusion roller can be changed, and when the diameter of the extrusion roller is increased, the ultrafiltration membrane body is extruded at the position where the first wallboard and the net body are in lap joint; along the lengthening direction of the first liquid cavity, the outer side of the net body is also provided with a second liquid cavity respectively; the front and rear opposite second wall plates of the first liquid cavity vertical to the paper surface extend outwards and cover the front and rear end surfaces of the net body roller, the second liquid cavity and the squeeze roller; the net body roller and the squeeze roller are matched with the inner surface of the second wallboard in a sealing sliding fit manner;
an ultrafiltration piston is arranged in the first liquid cavity corresponding to the ultrafiltration mechanism in a sealing sliding manner; the ultrafiltration piston is fixedly connected with the ultrafiltration piston rod; a first return spring is arranged between the ultrafiltration piston and the spherical shell; a first liquid inlet which can be opened and closed is formed in the second wall plate between the ultrafiltration piston and the net body roller; a first liquid outlet which can be opened and closed is arranged on the second liquid cavity corresponding to the ultrafiltration mechanism;
a rotary steering shell is fixedly arranged in the first liquid cavity corresponding to the filter membrane regeneration mechanism; a cylindrical piston is sleeved in the steering shell; an annular piston is arranged in the arc-shaped space between the cylindrical piston and the steering shell in a sealing sliding manner; the annular piston, the steering shell and the regeneration piston are enclosed to form a steering liquid cavity; the steering liquid cavity is filled with pressure oil; the annular piston is linked with a living plug over and over again through a plurality of connecting rods; the regeneration piston is hermetically and slidably sleeved in a first liquid cavity between the corresponding net body roller and the steering shell; a second return spring is arranged between the regeneration piston and the support rod of the fixed steering shell; a second liquid outlet which can be opened and closed is formed in a second wall plate between the regeneration piston and the net body roller; and a second liquid inlet which can be opened and closed is arranged on the second liquid cavity corresponding to the filter membrane regeneration mechanism.
The splicing end face of the arc panel is embedded with a sealing rubber pad; at least one arc panel is provided with a water filling port which can fill water into the spherical inner cavity in one way; the bottom of the spherical shell is provided with a water outlet which can be opened and closed.
The extrusion roller comprises a second rotating shaft positioned at the center, and an electric heating layer, a thermosensitive solid layer, a heat insulation layer and a rubber layer which are sequentially sleeved outside the second rotating shaft; the electric heating layer comprises semiconductor refrigerating sheets densely arranged along the inner circumferential surface of the thermosensitive solid layer; the hot end or the cold end of the semiconductor refrigerating sheet is contacted with the inner surface of the thermosensitive solid layer; an external circuit can control the on-off state and the current direction of the semiconductor refrigeration piece so as to switch the hot end and the cold end of the semiconductor refrigeration piece.
Wherein the ultrafiltration membrane body comprises an ultrafiltration membrane and a porous tensile material which are sequentially laminated; the porous tensile material is porous rubber or porous polymer composite material.
The beneficial effects of the invention are as follows:
1. the invention can improve the extraction rate of curcumin by combining technical means of hot air drying, protease enzymolysis and starch adsorption in sequence, and improves the storage stability and the anti-inflammatory and antioxidant effects by encapsulating the soybean protein and the soybean oil.
2. The invention improves the solubility of the cranberry powder by a degradation method of cellulase combined with protease; wherein, the enzyme complex of cellulase and protease can improve the solubility of the cranberry powder in various solution systems for the enzymolysis product of the cranberry residue and the alcohol extract of the fine residue.
3. The invention provides a novel anti-inflammatory composition which is a combination of turmeric powder, cranberry powder, licorice powder and astragalus powder, has stronger anti-inflammatory effect and anti-oxidation effect, and can be used in health care products.
4. Aiming at the problem that ultrafiltration membranes of the existing ultrafiltration device are easy to block and influence the ultrafiltration effect to cause a short plate with production efficiency, in order to compensate the short plate with production efficiency and improve the production effect, the ultrafiltration device is designed in a targeted way, the ultrafiltration mechanism and the filter membrane regeneration mechanism are combined and integrated, synchronous power output is provided through a central sphere device, and the circulation movement of an ultrafiltration membrane body is realized through the design of a guide roller, an extrusion roller, a net body roller and an integral structure, so that the dynamic ultrafiltration and regeneration of the ultrafiltration membrane body are realized, the filtration and regeneration processes of the ultrafiltration membrane body form dynamic circulation, on one hand, the real-time filtration effect of the ultrafiltration membrane body can be ensured, the integral production efficiency is improved, on the other hand, the high-efficiency utilization of the ultrafiltration membrane can be ensured, the service life of the ultrafiltration membrane is prolonged, and the use cost is further spread; the central sphere is utilized to realize synchronous driving of the ultrafiltration mechanism and the filter membrane regeneration mechanism, and the steering of the regeneration piston is realized by the design of a steering liquid cavity and the like, so that the central sphere is utilized to synchronously drive the ultrafiltration piston rod and the regeneration piston rod to act, but the synchronous reverse action of the ultrafiltration piston and the regeneration piston can be realized, the process that the ultrafiltration mechanism extrudes stock solution from a first liquid cavity to a second liquid cavity through the ultrafiltration membrane body is realized, and the ultrafiltration effect is realized; meanwhile, the process that the filter membrane regeneration mechanism absorbs clean water from the second liquid cavity through the ultrafiltration membrane body by the first liquid cavity is realized, so that the backflushing effect on the ultrafiltration membrane body is realized, and the holes blocked by the ultrafiltration membrane body are cleaned.
5. The central sphere is driven by using shock waves generated in water by the liquid-electricity effect as power, has high corresponding speed, safety, controllability and good sealing performance, can generate driving force from multiple directions uniformly, and can synchronously extrude the arc panel outwards, and the design of the arc panel can be spliced again to form a spherical inner cavity under the action of restoring force of the first restoring spring and the second restoring spring after the driving force disappears, so that the next extrusion ultrafiltration and regeneration are prepared; in the recovery period, the ultrafiltration membrane body is driven to move for a certain distance through the guide roller, the extrusion roller and the net body roller; and because ultrafiltration, regeneration and movement of the ultrafiltration membrane body are performed in a staggered manner, the guide roller, the squeeze roller and the net body roller do not need to rotate in the ultrafiltration and regeneration process, so that the complexity of equipment is reduced, the sealing is convenient, and the occurrence of water leakage is reduced effectively.
6. The extrusion roller adopts a multilayer structure, utilizes the sensitivity of a thermosensitive material to temperature, and utilizes the expansion effect and the contraction effect to realize the change of the diameter of the extrusion roller, so that the extrusion roller can extrude an ultrafiltration membrane body to play a role in sealing and fixing during ultrafiltration and regeneration backflushing, and can reduce the diameter to obtain proper friction force for the ultrafiltration membrane body when the ultrafiltration membrane body is required to move; the semiconductor refrigeration sheet array is adopted to control the expansion and contraction of the heat-sensitive solid layer, so that the expansion and contraction of the heat-sensitive solid layer can be accurately controlled, and the cold and hot ends can be quickly and simply switched, so that the temperature of the heat-sensitive solid layer can be quickly raised and the temperature of the heat-sensitive solid layer can be quickly lowered, the temperature of the heat-sensitive solid layer can be quickly adjusted, and the diameter of the extrusion roller can be further adjusted; the ultrafiltration membrane body is designed to be composed of an ultrafiltration membrane and a porous tensile material which are sequentially laminated, so that the ultrafiltration effect is guaranteed, and meanwhile, the ultrafiltration membrane body has enough tensile strength and compressive strength, is not only an ultrafiltration structure, but also a tensioning structure, and is beneficial to realizing a circulation function and prolonging the service life.
Drawings
FIG. 1 is a process schematic diagram of the curcumin extraction method of the present invention;
FIG. 2 is a schematic diagram of the structure of the ultrafiltration device of the present invention;
FIG. 3 is a schematic diagram of the structure of the ultrafiltration device of the present invention in operation;
FIG. 4 is a schematic view of the structure of the center sphere of the present invention;
FIG. 5 is a schematic diagram of the structure of the ultrafiltration mechanism of the present invention;
FIG. 6 is a schematic diagram of a filter membrane regeneration mechanism according to the present invention;
FIG. 7 is a schematic view showing the structure of the squeeze roll of the present invention;
FIG. 8 is a schematic structural view of an ultrafiltration membrane body according to the present invention.
The reference numerals in the drawings are as follows:
1. a center sphere; 11. a spherical shell; 12. an arc panel; 13. a spherical inner cavity; 14. a discharge electrode; 15. ultrafiltration piston rod; 16. regenerating a piston rod; 2. an ultrafiltration mechanism; 21. a first liquid chamber; 211. a first wall plate; 22. a web roll; 221. a first rotating shaft; 222. a hollow cylindrical mesh body; 223. a connecting rod; 23. a guide roller; 24. a squeeze roll; 241. a second rotating shaft; 242. an electrical heating layer; 243. a thermosensitive solid layer; 244. a thermal insulation layer; 245. a rubber layer; 246. a semiconductor refrigeration sheet; 25. a second liquid chamber; 26. an ultrafiltration piston; 27. a first return spring; 3. a filter membrane regeneration mechanism; 31. a steering housing; 32. a cylindrical piston; 33. an annular piston; 34. a steering fluid chamber; 35. a connecting rod; 36. a regeneration piston; 37. a second return spring; 38. a support rod; 4. an ultrafiltration membrane body; 41. an ultrafiltration membrane; 42. porous tensile material.
Detailed Description
The present invention will be described in detail below with reference to specific embodiments for better explaining the present invention.
Example 1
The preparation method of the cranberry powder provided by the embodiment comprises the following steps:
s1, squeezing fresh cranberries, and separating cranberry juice and cranberry residues by using a 200-mesh gauze;
s2, adding complex enzyme into the cranberry residue, and carrying out enzymolysis for 6min at 30 ℃; the compound enzyme is prepared by mixing cellulase and subtilisin according to the enzyme activity ratio of 1:1.5, wherein the adding amount of the compound enzyme is 200U/g of the weight of fresh cranberry, and the enzymolysis liquid and the enzymolysis residue are obtained by separating the mixture by using a 200-mesh gauze after enzymolysis;
s3, centrifuging the cranberry juice for 2min to obtain supernatant and precipitate, ultrafiltering the supernatant to obtain fine residue and juice, wherein the pore size of an ultrafiltration membrane is 90 nm;
mixing the fine slag and the precipitate, adding ethanol which is 1.3 times of the weight of the fresh cranberry, extracting the mixture with ethanol to obtain an ethanol extract, wherein the mass concentration of the ethanol is 45%, carrying out rotary distillation on the ethanol extract to remove ethanol, adding water which is 7 times of the mass of the ethanol extract, and adding medlar polysaccharide which is 0.6 time of the mass of the ethanol extract, and uniformly dispersing to obtain a suspension;
s4, homogenizing the obtained juice and suspension, mixing with the enzymolysis solution, and drying to obtain the cranberry powder.
Example 2
The preparation method of the cranberry powder provided by the embodiment comprises the following steps:
s1, squeezing fresh cranberries, and separating cranberry juice and cranberry residues by using a 200-mesh gauze;
s2, adding complex enzyme into the cranberry residue, and carrying out enzymolysis for 2min at 32 ℃; the compound enzyme is prepared by mixing cellulase and subtilisin according to an enzyme activity ratio of 1:2, wherein the adding amount of the compound enzyme is 100U/g of the weight of fresh cranberry, and after enzymolysis, the enzymolysis liquid and enzymolysis residues are obtained by separating with a 200-mesh gauze;
s3, centrifuging the cranberry juice at 3500r/min for 1min to obtain supernatant and precipitate, ultrafiltering the supernatant to obtain fine residue and juice, wherein the pore size of the ultrafiltration membrane is 100nm;
mixing the fine slag and the precipitate, adding ethanol which is 1 time of the weight of the fresh cranberry, extracting the mixture with ethanol to obtain an ethanol extract, wherein the mass concentration of the ethanol is 50%, carrying out rotary distillation on the ethanol extract to remove ethanol, adding water which is 5 times of the mass of the ethanol extract, and adding medlar polysaccharide which is 0.8 time of the mass of the ethanol extract, and uniformly dispersing to obtain a suspension;
s4, homogenizing the obtained juice and suspension, mixing with the enzymolysis solution, and drying to obtain the cranberry powder.
Example 3
The preparation method of the cranberry powder provided by the embodiment comprises the following steps:
s1, squeezing fresh cranberries, and separating cranberry juice and cranberry residues by using a 200-mesh gauze;
s2, adding complex enzyme into the cranberry residue, and carrying out enzymolysis for 10min at 28 ℃; the compound enzyme is prepared by mixing cellulase and subtilisin according to an enzyme activity ratio of 1:1, wherein the adding amount of the compound enzyme is 250U/g of the weight of fresh cranberry, and after enzymolysis, the enzymolysis liquid and enzymolysis residues are obtained by separating with a 200-mesh gauze;
s3, centrifuging the cranberry juice for 3min at 3000r/min to obtain supernatant and precipitate, ultrafiltering the supernatant to obtain fine residue and juice, wherein the pore size of an ultrafiltration membrane is 50 nm;
mixing the fine slag and the precipitate, adding ethanol which is 1.5 times of the weight of the fresh cranberry, extracting the mixture with ethanol to obtain an ethanol extract, wherein the mass concentration of the ethanol is 40%, carrying out rotary distillation on the ethanol extract to remove ethanol, adding water which is 10 times of the mass of the ethanol extract, and adding medlar polysaccharide which is 0.5 time of the mass of the ethanol extract, and uniformly dispersing to obtain a suspension;
s4, homogenizing the obtained juice and suspension, mixing with the enzymolysis solution, and freeze-drying to obtain the cranberry powder.
Example 4
Referring to fig. 1, the preparation method of turmeric powder provided in this embodiment includes the following steps:
s1, adding 12 times of water into turmeric powder which is dried by hot air until the content is less than 10%, decocting for 15 minutes, adding neutral protease for enzymolysis for 13 minutes, and filtering to obtain an enzymolysis solution, wherein the addition amount of the neutral protease is 3300U/g turmeric powder, and the enzymolysis temperature is 45 ℃;
s2, sequentially adding starch and soybean oil into the enzymolysis liquid for adsorption, and centrifuging to obtain a precipitate; the addition amount of starch is 4% of the weight of turmeric powder, and the addition amount of amylase is 1400U/g of the weight of starch;
and S3, adding amylase into the precipitate for enzymolysis for 2min, extracting with ethanol, removing ethanol to obtain an extracting solution, adding soybean protein isolate with the mass of 2% of turmeric, heating at 100 ℃ for 5min, and drying until the water content is less than 10% to obtain turmeric powder.
Example 5
Referring to fig. 1, the preparation method of turmeric powder provided in this embodiment includes the following steps:
s1, adding 15 times of water into turmeric powder which is dried by hot air until the content is less than 10%, decocting for 10 minutes, adding neutral protease for enzymolysis for 15 minutes, and filtering to obtain an enzymolysis solution, wherein the addition amount of the neutral protease is 3200U/g turmeric powder, and the enzymolysis temperature is 45 ℃;
s2, sequentially adding starch and soybean oil into the enzymolysis liquid for adsorption, and centrifuging to obtain a precipitate; the addition amount of starch is 3% of the weight of turmeric powder, and the addition amount of amylase is 1600U/g of the weight of starch;
and S3, adding amylase into the precipitate for enzymolysis for 1min, extracting with ethanol, removing ethanol to obtain an extracting solution, adding soybean protein isolate with the mass of 3% of turmeric, heating at 100 ℃ for 3min, and drying until the water content is less than 10% to obtain turmeric powder.
Example 6
Referring to fig. 1, the preparation method of turmeric powder provided in this embodiment includes the following steps:
s1, adding 10 times of water into turmeric powder which is dried by hot air until the content is less than 10%, decocting for 20 minutes, adding neutral protease for enzymolysis for 10 minutes, and filtering to obtain an enzymolysis solution, wherein the addition amount of the neutral protease is 3500U/g turmeric powder, and the enzymolysis temperature is 40 ℃;
s2, sequentially adding starch and soybean oil into the enzymolysis liquid for adsorption, and centrifuging to obtain a precipitate; the addition amount of the starch is 5% of the weight of the turmeric powder, and the addition amount of the amylase is 1300U/g of the weight of the starch;
and S3, adding amylase into the precipitate for enzymolysis for 3min, extracting with ethanol, removing ethanol to obtain an extracting solution, adding soybean protein isolate with the mass of 1% of turmeric, heating at 100 ℃ for 6min, and drying until the water content is less than 10% to obtain turmeric powder.
The extract prepared by the preparation method of the embodiments 4-6 of the invention can improve the anti-inflammatory and antioxidant effects of the prepared extract after the soybean protein isolate is added for heating treatment.
Example 7
The novel anti-inflammatory composition provided in this embodiment is prepared from 3 parts by weight of turmeric powder obtained in embodiment 4, 5 parts by weight of cranberry powder obtained in embodiment 1, 3 parts by weight of licorice powder and 6 parts by weight of astragalus powder, and specifically comprises the following preparation methods: mixing Curcuma rhizome powder, cranberry powder, glycyrrhrizae radix powder and radix astragali powder, adding 33 times of water to dissolve, and heating to boiling state under normal pressure for 4min to obtain antiinflammatory composition.
To verify the anti-inflammatory effect of the present invention, the following test was performed,
test one
Cranberry powder prepared without adding suspension in step S4 of the method of example 1 was used as comparative example 1;
in the step S3, the medlar polysaccharide is not added to prepare cranberry powder serving as a comparative example 2;
and S4, performing rotary distillation on the alcohol extract obtained in the step S3 to remove ethanol, mixing the alcohol extract with the obtained juice and the enzymolysis liquid, and performing freeze drying to obtain cranberry powder serving as comparative example 3, and measuring the dissolution capacity in an oil phase.
The method is characterized in that the solubility of the cranberry powder in the soybean oil is determined by a dynamic method through test:
in 100g of soybean oil, the soybean oil,
the dissolution power of the cranberry powder of example 1 was 45g;
the dissolution power of the cranberry powder of comparative example 1 was 5g;
the dissolution power of the cranberry powder of comparative example 2 was 25g;
the dissolution power of the cranberry powder of comparative example 3 was 30g;
to verify the dissolution capacity of the cranberry powder obtained in the examples of the present invention, the following tests were conducted,
cranberry powder prepared without adding suspension in step S4 of the method of example 1 was used as comparative example 1;
in the step S3, the medlar polysaccharide is not added to prepare cranberry powder serving as a comparative example 2;
and S4, performing rotary distillation on the alcohol extract obtained in the step S3 to remove ethanol, mixing the alcohol extract with the obtained juice and the enzymolysis liquid, and performing freeze drying to obtain cranberry powder serving as comparative example 3, and measuring the dissolution capacity in an oil phase.
The method is characterized in that the solubility of the cranberry powder in the soybean oil is determined by a dynamic method through test:
in 100g of soybean oil, the soybean oil,
the dissolution power of the cranberry powder of example 1 was 45g;
the dissolution power of the cranberry powder of comparative example 1 was 5g;
the dissolution power of the cranberry powder of comparative example 2 was 25g;
the dissolution power of the cranberry powder of comparative example 3 was 30g;
from the above measurement, the addition of the matrimony vine polysaccharide can improve the dissolution capability of the cranberry powder in the oil phase, and the invention can improve the dissolution capability of the cranberry powder in the oil phase by the enzymolysis liquid obtained by the enzymolysis treatment of the cranberry slag and the alcohol extract of the fine slag; the dissolving capacity of the suspension prepared by the special alcohol extract is further improved after the homogenization treatment of the suspension and the enzymolysis liquid. It is known that the suspension prepared from the substances obtained by enzymolysis and the alcohol extract is homogenized, so that the substances with better oil phase dissolution and the substances which are not easy to dissolve in the oil phase are mutually chelated to form two-phase soluble substances, thereby improving the dissolution capacity of the substances in the oil phase.
Because of its good dissolving power, the cranberry powder can be compatible with the turmeric powder produced by the present invention to form a stable dispersion.
Test II
The antioxidant and anti-inflammatory effects of example 7 of the present invention were measured.
Comparative example 4
Other points are as in example 7, except that: no cranberry powder was added.
Comparative example 5
Other points are as in example 7, except that: turmeric powder was not added.
Comparative example 6
Other points are as in example 7, except that: in the preparation method of the turmeric powder, soybean oil is not added for adsorption.
Comparative example 7
Other points are as in example 7, except that: in the preparation method of the turmeric powder, starch is not added for adsorption.
The anti-inflammatory composition obtained in example 7 of the present invention has an OH clearance of 71.6%;
the anti-inflammatory composition obtained in comparative example 4 has an OH clearance of 56.4%;
the anti-inflammatory composition obtained in comparative example 5 has an OH clearance of 42.5%;
the anti-inflammatory composition obtained in comparative example 6 had an OH-elimination rate of 62.8%.
Furthermore, the present invention finds by measurement that: the curcumin content in the turmeric powder obtained in comparative example 6 was 81.89% of the curcumin content in the turmeric powder of example 4, and the curcumin content in the turmeric powder obtained in comparative example 7 was 20.15% of the curcumin content in the turmeric powder of example 4.
Referring to fig. 2 to 8, the ultrafiltration treatment of S3 in examples 1 to 3 was performed by a device of special design comprising a central sphere 1, ultrafiltration mechanisms 2 disposed on the left and right sides of the central sphere 1, and filter membrane regeneration mechanisms 3 disposed on the upper and lower sides of the central sphere 1; the center sphere 1 comprises a spherical shell 11 and four cambered surface plates 12 arranged in the spherical shell 11; the four cambered surface plates 12 are spliced and enclosed to form a spherical inner cavity 13; the middle part of the spherical inner cavity 13 is provided with a discharge electrode 14; the outer surfaces of the left arc panel 12 and the right arc panel 12 are fixedly connected with an ultrafiltration piston rod 15 respectively; the outer surfaces of the upper arc panel 12 and the lower arc panel 12 are fixedly connected with a plug rod 16 for life again and again respectively;
the ultrafiltration mechanism 2 and the regeneration mechanism 3 respectively comprise a rectangular first liquid cavity 21 which is lengthened in the left-right direction and lengthened in the up-down direction; one end of the first liquid cavity 21 is connected with the spherical shell 11, and the other end is provided with a net body roller 22; the net body roller 22 comprises a first rotating shaft 221 positioned at the center and a hollow cylindrical net body 222 sleeved outside the first rotating shaft 221 at intervals; the first rotating shaft 221 is connected with the hollow cylindrical net body 222 through a plurality of connecting rods 223; one end of the two opposite first wall plates 211 of the first liquid cavity 21 is abutted against the surface of the net body 222; the first wall plate 211 is wedge-shaped at the position where the first wall plate is lapped with the surface of the net body 222; a guide roller 23 is respectively arranged at the included angle between two adjacent first liquid cavities 21; an ultrafiltration membrane body 4 is sequentially tensioned along the surface of the net body 222 and the guide roller 23, and the whole body is tensioned into a cross shape; a squeeze roller 24 is respectively arranged on the first wall plate 211 and the net body 222; the diameter of the squeeze roll 24 can be changed, and when the diameter of the squeeze roll 24 is increased, the ultrafiltration membrane body 4 is squeezed at the position where the first wall plate 211 and the net body 222 are in contact; along the lengthening direction of the first liquid cavity 21, the outer side of the net body 222 is also provided with a second liquid cavity 25 respectively; the first liquid cavity 21 extends outwards perpendicular to the front and rear opposite second wall plates of the paper surface and covers the front and rear end surfaces of the net body roller 22, the second liquid cavity 25 and the squeeze roller 24; the net body roller 22 and the squeeze roller 24 are matched with the inner surface of the second wall plate in a sealing sliding fit manner;
an ultrafiltration piston 26 is arranged in the first liquid cavity 21 corresponding to the ultrafiltration mechanism 2 in a sealing and sliding manner; the ultrafiltration piston 26 is fixedly connected with the ultrafiltration piston rod 15; a first return spring 27 is arranged between the ultrafiltration piston 26 and the spherical housing 11; a first liquid inlet which can be opened and closed is arranged on the second wall plate between the ultrafiltration piston 26 and the net body roller 22; a first liquid outlet which can be opened and closed is arranged on the second liquid cavity 25 corresponding to the ultrafiltration mechanism 2;
a rotary steering shell 31 is fixedly arranged in the first liquid cavity 21 corresponding to the filter membrane regeneration mechanism 3; a cylindrical piston 32 is sleeved in the steering housing 31; an annular piston 33 is arranged in a sealed sliding manner in the arc-shaped space between the cylindrical piston 32 and the steering housing 31; the annular piston 33, the steering housing 31 and the regeneration piston 32 are enclosed to form a steering liquid chamber 34; the steering liquid chamber 34 is filled with pressure oil; the annular piston 33 is linked with a living plug 36 through a plurality of connecting rods 35; the regenerating piston 36 is hermetically and slidably sleeved in the first liquid cavity 21 between the corresponding net body roller 22 and the steering shell 31; a second return spring 37 is arranged between the regenerating piston 36 and a strut 38 of the fixed steering housing 31; a second liquid outlet which can be opened and closed is arranged on a second wall plate between the regenerating piston 36 and the net body roller 22; the second liquid cavity 25 corresponding to the filter membrane regeneration mechanism 3 is provided with a second liquid inlet which can be opened and closed.
Further, a sealing rubber pad is embedded on the spliced end face of the cambered surface plate 12; at least one arc panel 12 is provided with a water filling port which can fill water into the spherical cavity 13 in one direction; the bottom of the spherical shell 11 is provided with a water outlet which can be opened and closed.
Further, the squeeze roller 24 includes a second rotating shaft 241 at the center, and an electric heating layer 242, a thermosensitive solid layer 243, a heat insulating layer 244 and a rubber layer 245 sequentially sleeved outside the second rotating shaft 241; the electric heating layer 242 includes semiconductor refrigerating sheets 246 densely arranged along the inner circumferential surface of the thermosensitive solid layer 243; the hot or cold end of the semiconductor refrigeration sheet 246 is in contact with the inner surface of the thermosensitive solid layer 243; an external circuit may control the on-off and current direction of the semiconductor refrigeration piece 246 to switch the hot and cold sides of the semiconductor refrigeration piece 246.
Further, the ultrafiltration membrane body 4 includes an ultrafiltration membrane 41 and a porous tensile material 42 laminated in this order.
Further, the porous tensile material 42 is a porous rubber or a porous polymer composite.
The working principle of the device adopted by the ultrafiltration treatment is as follows:
initially, the first liquid cavity 21 corresponding to the ultrafiltration mechanism 2 is filled with stock solution, and the second liquid cavity 25 corresponding to the filter membrane regeneration mechanism 3 is filled with clean water; the semiconductor refrigerating sheet 242 of the electric heating layer 242 in the squeeze roller 24 heats the thermosensitive solid layer 243, the thermosensitive fixing layer 243 is heated and expanded, the diameter of the squeeze roller 24 is increased, and the ultrafiltration membrane body 4 is tightly squeezed on the first wall plate 211
In a static state, four cambered plates 12 are enclosed to form a spherical shell shape, the inner periphery of the cambered plates is synthesized into a spherical inner cavity 13, and water is filled into the spherical inner cavity 13 through a water filling port; the external circuit applies a high-pressure electric field in the spherical cavity 13 through the discharge electrode 14 to generate a liquid-electricity effect, and the high-pressure electric field passes through the liquid, so that the liquid in the channel is rapidly vaporized and expanded due to the instant release of huge energy in the discharge channel, thereby generating an outward impact force; the impact forces respectively push the arc panel 12 to move outwards, so that the ultrafiltration piston rod 15 and the regeneration piston rod 16 are respectively driven to move outwards;
the ultrafiltration piston rod 15 drives the ultrafiltration piston 26 to move outwards, so that the stock solution in the first liquid cavity 21 of the ultrafiltration mechanism 2 is pushed to extrude towards the corresponding net body roller 22 and the ultrafiltration membrane body 4 covered on the net body roller 22, so that small molecular solutes and solvents pass through the ultrafiltration membrane body 4 to enter the corresponding second liquid cavity 25, and macromolecular solutes and impurities cannot permeate and remain in the first liquid cavity 21, after the completion, under the action of the first return spring 27, the ultrafiltration piston 26 is retracted, and at the moment, the liquid in the first liquid cavity 21 and the second liquid cavity 25 is respectively discharged, and the first liquid cavity 21 is refilled with the liquid to be ultrafiltered.
The regenerating piston rod 16 drives the cylindrical piston 26 to move outwards, extrudes liquid in the steering liquid cavity 24, and the liquid in the steering liquid cavity 24 is pressed to push the annular piston 33 to move towards the side of the central sphere 1; the annular piston 33 drives the regeneration piston 36 to move to the side of the central sphere 1 through the connecting rod 35, so that negative pressure is formed in the first liquid cavity 21 of the filter membrane regeneration mechanism 3, clean water in the corresponding second liquid cavity 25 moves to the first liquid cavity 21, in the process, the ultrafiltration membrane body 4 is subjected to backflushing cleaning, impurities blocked and attached to the ultrafiltration membrane body 4 are filled into the corresponding first liquid cavity 25, after the completion, the regeneration piston 36 is retracted under the action of the second return spring 37, at the moment, liquid in the first liquid cavity 21 is discharged, and the corresponding second liquid cavity 25 is refilled with clean water.
During the process of withdrawing the ultrafiltration piston 26 and the breeding piston 36, the cold and hot ends of the semiconductor refrigeration piece 246 are reversed, the thermosensitive solid layer 243 is contracted by cooling, so that the diameter of the squeeze roll 24 is reduced to a preset size, and at this time, the rubber layer 245 on the surface of the squeeze roll 24 has proper extrusion force on the ultrafiltration membrane body 4.
At this time, one or more of the squeeze roll 24, the guide roll 23 and the net body roll 22 has active power, and can drive the tensioned ultrafiltration membrane body 4 to move, and the moving distance of each time is the length of the single hollow cylindrical net body 222 corresponding to the coating ultrafiltration mechanism 2, so that the ultrafiltration process of the ultrafiltration membrane body 4 during ultrafiltration again after each movement can be ensured to be smooth.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (6)
1. The natural anti-inflammatory composition is characterized by comprising 2-5 parts by weight of turmeric powder, 4-6 parts by weight of cranberry powder, 2-5 parts by weight of licorice powder and 4-8 parts by weight of astragalus powder;
the preparation of the cranberry powder comprises the following steps:
squeezing fresh cranberry to obtain juice and residue;
the method comprises the steps of performing enzymolysis on cellulase and protease of cranberry residues according to a mixed complex enzyme with an enzyme activity ratio of 1:1-2, and separating to obtain an enzymolysis solution and enzymolysis residues, wherein the addition amount of the complex enzyme is 100-250U/g of the weight of fresh cranberry, the enzymolysis temperature is 28-32 ℃, and the enzymolysis time is 2-10 min;
ultrafiltration treatment of cranberry juice, separating fine slag and juice;
ethanol extraction of the fine slag to obtain an ethanol extract, ethanol removal treatment and water addition to prepare a suspension;
homogenizing the suspension and juice, mixing with enzymolysis solution, and drying to obtain cranberry powder.
2. The natural anti-inflammatory composition of claim 1, wherein: centrifuging for 1-3 min at 3000 r/min-3500 r/min before ultrafiltration; the pore size of the ultrafiltration membrane subjected to ultrafiltration treatment is 50-100 nm; the protease is subtilisin; the concentration of the ethanol is 40-50%, and the addition amount of the ethanol is 1-1.5 times of the weight of the fresh cranberry; the ratio of the alcohol extract to the water is 1:5-10.
3. The natural anti-inflammatory composition according to claim 1, wherein the device for ultrafiltration treatment used in the preparation method of the cranberry powder comprises a central sphere (1), ultrafiltration mechanisms (2) arranged on the left and right sides of the central sphere (1) and filter membrane regeneration mechanisms (3) arranged on the upper and lower sides of the central sphere (1); the center sphere (1) comprises a spherical shell (11) and four cambered surface plates (12) arranged in the spherical shell (11); the four cambered surface plates (12) are spliced and enclosed to form a spherical inner cavity (13); a discharge electrode (14) is arranged in the middle of the spherical inner cavity (13); the outer surfaces of the left arc panel (12) and the right arc panel (12) are respectively fixedly connected with an ultrafiltration piston rod (15); the outer surfaces of the upper arc panel (12) and the lower arc panel are respectively fixedly connected with a plug rod (16) for life;
the ultrafiltration mechanism (2) and the regeneration mechanism (3) respectively comprise a rectangular first liquid cavity (21) which is lengthened in the left-right direction and lengthened in the up-down direction; one end of the first liquid cavity (21) is connected with the spherical shell (11), and the other end of the first liquid cavity is provided with a net body roller (22); the net body roller (22) comprises a first rotating shaft (221) positioned at the center and a hollow cylindrical net body (222) sleeved outside the first rotating shaft (221) at intervals; the first rotating shaft (221) is connected with the hollow cylindrical net body (222) through a plurality of connecting rods (223); one end of each of the two opposite first wall plates (211) of the first liquid cavity (21) is abutted against the surface of the net body (222); the first wall plate (211) is wedge-shaped at the position where the first wall plate is lapped with the surface of the net body (222); a guide roller (23) is respectively arranged at the included angle between two adjacent first liquid cavities (21); an ultrafiltration membrane body (4) is sequentially tensioned along the surface of the net body (222) and the guide roller (23), and the whole body is tensioned into a cross shape; the first wall plate (211) and the net body (222) are respectively provided with a squeeze roller (24) in a lap way; the diameter of the extrusion roller (24) can be changed, and when the diameter of the extrusion roller (24) is increased, the ultrafiltration membrane body (4) is extruded at the position where the first wall plate (211) and the net body (222) are in lap joint; along the lengthening direction of the first liquid cavity (21), the outer side of the net body (222) is also provided with a second liquid cavity (25) respectively; the front and rear opposite second wall plates of the first liquid cavity (21) perpendicular to the paper surface extend outwards and cover the front and rear end surfaces of the net body roller (22), the second liquid cavity (25) and the squeeze roller (24); the net body roller (22) and the squeeze roller (24) are matched with the inner surface of the second wallboard in a sealing sliding fit manner;
an ultrafiltration piston (26) is arranged in the first liquid cavity (21) corresponding to the ultrafiltration mechanism (2) in a sealing and sliding manner; the ultrafiltration piston (26) is fixedly connected with the ultrafiltration piston rod (15); a first return spring (27) is arranged between the ultrafiltration piston (26) and the spherical shell (11); a first liquid inlet which can be opened and closed is arranged on a second wall plate between the ultrafiltration piston (26) and the net body roller (22); a first liquid outlet which can be opened and closed is arranged on a second liquid cavity (25) corresponding to the ultrafiltration mechanism (2);
a rotary steering shell (31) is fixedly arranged in the first liquid cavity (21) corresponding to the filter membrane regeneration mechanism (3); a cylindrical piston (32) is sleeved in the steering shell (31); an annular piston (33) is arranged in the arc-shaped space between the cylindrical piston (32) and the steering shell (31) in a sealing sliding manner; the annular piston (33), the steering shell (31) and the regeneration piston (32) are enclosed to form a steering liquid cavity (34); the steering liquid cavity (34) is filled with pressure oil; the annular piston (33) is linked with a living plug (36) through a plurality of connecting rods (35); the regeneration piston (36) is hermetically and slidably sleeved in the first liquid cavity (21) between the corresponding net body roller (22) and the steering shell (31); a second return spring (37) is arranged between the regeneration piston (36) and a support rod (38) of the fixed steering shell (31); a second liquid outlet which can be opened and closed is arranged on a second wall plate between the regenerating piston (36) and the net body roller (22); and a second liquid inlet which can be opened and closed is arranged on a second liquid cavity (25) corresponding to the filter membrane regeneration mechanism (3).
4. A natural anti-inflammatory composition as claimed in claim 3, characterized in that the spliced end faces of the cambered plates (12) are embedded with sealing gaskets; at least one arc panel (12) is provided with a water filling port which can fill water into the spherical inner cavity (13) in one direction; the bottom of the spherical shell (11) is provided with a water outlet which can be opened and closed.
5. The natural anti-inflammatory composition according to claim 4, wherein the squeeze roll (24) comprises a second rotating shaft (241) at the center, and an electric heating layer (242), a thermosensitive solid layer (243), a heat insulating layer (244) and a rubber layer (245) which are sequentially sleeved outside the second rotating shaft (241); the electric heating layer (242) comprises semiconductor refrigerating sheets (246) densely arranged along the inner circumferential surface of the thermosensitive solid layer (243); the hot end or the cold end of the semiconductor refrigeration piece (246) is in contact with the inner surface of the thermosensitive solid layer (243); an external circuit controls the on-off and current direction of the semiconductor refrigeration sheet (246) to switch the hot and cold sides of the semiconductor refrigeration sheet (246).
6. The natural anti-inflammatory composition according to claim 5, wherein the ultrafiltration membrane body (4) comprises an ultrafiltration membrane (41) and a porous tensile material (42) laminated in this order; the porous tensile material (42) is porous rubber or porous polymer composite material.
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| CN104623670A (en) * | 2013-11-06 | 2015-05-20 | 高松 | Compositions Containing Enriched Natural Crocin and/or Crocetin, and Their Therapeutic or Nutraceutical Uses |
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