CN115624007A - Intelligent shrimp larvae detection method based on biological information technology - Google Patents
Intelligent shrimp larvae detection method based on biological information technology Download PDFInfo
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Abstract
The invention discloses an intelligent shrimp larva detection method based on a biological information technology, which comprises the following steps: conveying water of the shrimp fry farm to a statistical pond; weighing and counting the weight of the water in the tank; controlling the sampling box to collect water in the statistical pool; weighing the weight of the water in the sampling box; shooting towards one side surface of the sampling box by using a camera to identify the sampling number of the shrimp seeds; calculating the total number of the shrimp seeds in the statistical pool; detecting the liveness of the shrimp larvae in the statistical pond; putting a plurality of shrimp larvae samples into a collecting pipe containing an extraction solution, and uniformly dispersing and cracking; immersing the adsorption membrane in the extraction solution; and (3) soaking and washing the adsorption membrane by using a washing buffer solution, and placing the adsorption membrane in the PCR reaction solution to release nucleic acid. The invention does not need to manually obtain the shrimp larvae, can automatically convey the water of the shrimp larvae culturing farm to the counting pool for carrying out the shrimp larvae counting, has low labor cost, can carry out nucleic acid acquisition, is convenient for carrying out PCR amplification and the like so as to obtain the genetic information of the shrimp larvae sample, and is convenient for carrying out various detections.
Description
Technical Field
The invention relates to the technical field of shrimp larvae breeding, in particular to an intelligent shrimp larvae detection method based on a biological information technology.
Background
In the aquaculture process, the count of shrimp larvae is the indispensable link in the shrimp larvae trading market, consequently, the in-process such as stocking and selling of shrimp larvae need count, however, in the traditional mode, people all rely on the manual work to carry the water in the plant (including the shrimp larvae) to the container when carrying out the count of shrimp larvae, and adopt the camera to take a picture to this container, carry out image recognition in order to calculate the quantity of shrimp larvae at last, but this mode cost of labor is big, work efficiency is low, be unsuitable for the count of big batch shrimp larvae simultaneously (like several hundred or several thousand).
Disclosure of Invention
The invention mainly solves the technical problem of providing an intelligent shrimp larva detection method based on a biological information technology, which can automatically convey water of a shrimp larva farm to a statistic pool without manually acquiring shrimp larvae, is low in labor cost and high in working efficiency, can count the shrimp larvae in a large batch, and can count the shrimp larvae.
In order to solve the technical problems, the invention adopts a technical scheme that: the intelligent shrimp larvae detection method based on the biological information technology is characterized by comprising the following steps: step S101: conveying water in a shrimp fry farm to a statistical tank, wherein the shrimp fry farm is cultured with shrimp fries, the horizontal plane of the bottom surface of the shrimp fry farm is higher than the horizontal plane of the bottom surface of the statistical tank, an output port of the shrimp fry farm is communicated with an input port of the statistical tank through a first conveying pipeline, a first switch mechanism is arranged at the output port of the shrimp fry farm, and when the first switch mechanism is opened, the water in the shrimp fry farm automatically flows into the statistical tank through the first conveying pipeline; step S102: weighing the weight W1 of the water in the counting pool by using a first electronic scale, wherein the first electronic scale for weighing the weight of the water in the counting pool is arranged on the bottom surface of the counting pool; step S103: lowering a cartridge into the statistics pool and controlling the cartridge to collect water within the statistics pool and, after the cartridge collects water within the statistics pool, controlling the cartridge to rise away from a surface of the water within the statistics pool; step S104: weighing the weight W2 of the water in the sampling box by using a second electronic scale, wherein the bottom surface of the sampling box is provided with the second electronic scale for weighing the weight of the water in the sampling box; step S105: taking a picture of one side of the sampling box by using a camera to obtain a sampling picture, and then controlling the sampling box to descend and discharge water in the sampling box; step S106: identifying the sampling number V1 of the shrimp larvae through the sampling pictures; step S107: controlling the cartridge to move to the next collection point; step S108: repeatedly executing the steps S103-S106 to obtain a plurality of sampling numbers V1 of the shrimp larvae, and calculating the average value V of the shrimp larvae through the plurality of sampling numbers V1, wherein the weight of the collected water is the same when the sampling box descends to the statistic pool each time; step S109: calculating the total number S of the shrimp fries in the counting pool through a formula S = W1/W2V; step S110: detecting the activity of the shrimp larvae in the statistical pool; step S111: randomly extracting a plurality of shrimp larvae samples from the statistic pool, putting the shrimp larvae samples into a collecting pipe containing an extraction solution, and uniformly dispersing and cracking, wherein the extraction solution comprises the following components: tris buffer, 1M guanidinium isothiocyanate, 0.5wt% TritonX100, 1wt% Tween-20, 60 μ g/mL proteinase K, said Tris buffer having a pH of 8.0; step S112: dipping an adsorption film in the extraction solution, wherein the adsorption film is a cellulose film treated by anion resin containing quaternary ammonium salt; step S113: the adsorption membrane is washed with a washing buffer, which is Tris buffer containing 0.1wt% Tween-20, and placed in the eluent or PCR reaction solution to release the nucleic acids.
Further, in step S113, releasing the nucleic acid under the condition of pH =8.3, where a first output port of the counting chamber is communicated with a recovery chamber through a second delivery pipe, a bottom surface of the counting chamber is located at a higher level than a bottom surface of the recovery chamber, the first output port of the counting chamber is provided with a second switch mechanism, and when the second switch mechanism is turned on, water in the counting chamber automatically flows into the recovery chamber through the second delivery pipe, and the method further includes: and after the total quantity S of the shrimp seeds in the sampling box is calculated, controlling the second switch mechanism to be switched on so that the water in the counting tank automatically flows into the recovery tank through a second conveying pipeline.
Furthermore, a second output port of the counting pool is communicated with the selling box through a third conveying pipeline, the horizontal plane of the bottom surface of the counting pool is higher than the horizontal plane of the bottom surface of the selling box, a third switching mechanism is arranged at the second output port of the counting pool, when the third switching mechanism is opened, water in the counting pool automatically flows into the selling box through the third conveying pipeline, and the method further comprises the following steps: after the total number S of the shrimp seeds in the sampling box is calculated, judging whether the total number S of the shrimp seeds reaches a pre-sale number, wherein the pre-sale number is an artificial set value; and if the total quantity S of the shrimp seeds is determined to reach the pre-sale quantity, controlling the third switch mechanism to be opened so that the water in the counting pool automatically flows into the sales box through a third conveying pipeline.
Further, the method further comprises: if the total quantity S of the shrimp seeds is determined not to reach the pre-sale quantity, controlling the first switch mechanism to be opened so that the water in the shrimp seed farm automatically flows into the statistical pool through a first conveying pipeline; steps S102-S108 are repeatedly performed so that the total number S of shrimp larvae reaches the pre-sale number.
Furthermore, the top in statistics pond is provided with first lead screw, threaded connection and is in first connecting piece and fixed the setting in the first lead screw are in the first actuating cylinder of first connecting piece below, wherein the telescopic link orientation of first actuating cylinder statistics pond setting, just the telescopic link of first actuating cylinder with sample box fixed connection, in order to pass through first actuating cylinder control the sample box goes up and down.
Furthermore, a second connecting piece is connected to the first screw rod in a threaded manner, wherein the second connecting piece is connected with the first connecting piece through a first connecting rod, and a camera for taking pictures of one side face of the sampling box is arranged at the bottom of the second connecting piece.
Further, the sample box is the rectangle form, be equipped with the collection cavity in the sample box, the bottom of sample box be equipped with gather the collection mouth of cavity intercommunication, the bottom liftable of sample box is provided with and is used for sealing gather the sealed lid of mouth, just the sample box is close to the transparence is personally submitted to one side of camera, the sample box is kept away from the opposite side of camera is personally submitted opaque setting.
Further, the shell of first actuating cylinder extends and is equipped with the mounting bracket, be provided with the second actuating cylinder in the mounting bracket, the telescopic link orientation of second actuating cylinder statistics pond setting, the telescopic link of second actuating cylinder with sealed lid fixed connection, in order to pass through the second actuating cylinder drives sealed lid goes up and down, just sealed lid orientation the top surface of sampling box is equipped with the sealing silica gel pad that is the arc form.
Further, the intelligent shrimp larvae detection method based on the bioinformatics technology further comprises the following steps: when the sampling box descends and submerges on the water surface of the counting pool, the second driving cylinder drives the sealing cover to descend so that the sealing cover leaves the collecting opening, and after the sampling box is soaked for a preset time, the second driving cylinder drives the sealing cover to ascend so as to seal the collecting opening.
Further, the first switch mechanism comprises a first lifting plate arranged in an output port of the shrimp farm in a lifting manner and a third driving cylinder used for controlling the first lifting plate to lift, so that when the third driving cylinder drives the first lifting plate to lift, water in the shrimp farm automatically flows into the statistical pool, and when the third driving cylinder drives the first lifting plate to lower, the water in the shrimp farm stops flowing into the statistical pool; the second switch mechanism comprises a second lifting plate arranged in a first output port of the statistical pool in a lifting mode and a fourth driving cylinder used for controlling the second lifting plate to lift, so that when the fourth driving cylinder drives the second lifting plate to lift, water in the statistical pool automatically flows into the recovery pool, and when the fourth driving cylinder drives the second lifting plate to lower, the water in the statistical pool stops flowing into the recovery pool; the third switch mechanism comprises a third lifting plate and a fifth driving cylinder, the third lifting plate is arranged in an output port of the shrimp farm in a lifting mode, the fifth driving cylinder is used for controlling the third lifting plate to lift, when the fifth driving cylinder drives the third lifting plate to lift, water in the counting tank automatically flows to the inside of the selling box, when the fifth driving cylinder drives the third lifting plate to descend, the water in the counting tank stops flowing to the inside of the selling box.
The invention has the beneficial effects that: different from the situation of the prior art, the method does not need to acquire the shrimp seeds manually, can convey the water of the shrimp seed farm to the counting pool in an automatic mode, counts the shrimp seeds, is low in labor cost and high in working efficiency, can count the shrimp seeds in large batches, and can conveniently further perform PCR amplification and the like by randomly extracting a plurality of shrimp seed samples in the counting pool and acquiring nucleic acid so as to quickly acquire the genetic information of the shrimp seed samples and conveniently perform various detections.
Drawings
FIG. 1 is a schematic diagram of the shrimp fry counting system of the present invention;
FIG. 2 is a schematic diagram of the structure of the statistics pool of FIG. 1;
FIG. 3 is a schematic view of the cartridge of FIG. 1;
fig. 4 is a flow diagram of the intelligent shrimp larvae detection method based on the bioinformatics technology.
Detailed Description
Referring to fig. 1-3, the invention discloses a shrimp fry counting system, which comprises a shrimp fry farm 10, a counting pool 11, a sampling box 12, a recovery pool 13 and a sale box 14.
As shown in fig. 4, the intelligent shrimp larvae detection method based on the bioinformatics technology comprises the following steps:
step S101: the water of the shrimp farm 10 is delivered to a statistical pond 11.
It should be understood that the shrimp farm 10 is cultivated with shrimp larvae, and preferably, the bottom of the shrimp farm 10 is at a higher level than the bottom of the statistical pond 11.
As shown in fig. 1, an output port of the shrimp farm 10 is communicated with an input port of the counting tank 11 through a first conveying pipe 101, and preferably, the output port of the shrimp farm 10 is provided with a first switching mechanism, and when the first switching mechanism is turned on, water in the shrimp farm 10 automatically flows into the counting tank 11 through the first conveying pipe 101.
It should be understood that, because the bottom surface of the shrimp farm 10 is at a higher level than the bottom surface of the statistical pond 11, when the first switch mechanism is turned on, water in the shrimp farm 10 will automatically flow into the statistical pond 11 through the first conveying pipeline 101 due to gravity, and without manual operation, the shrimp in the shrimp farm 10 is automatically conveyed into the statistical pond 11 directly by means of gravity.
In this embodiment, first switch mechanism includes first lifter plate 102 of liftable setting in shrimp fry plant 10's delivery outlet and the third driving cylinder that is used for controlling first lifter plate 102 and goes up and down, when making the first lifter plate 102 of third driving cylinder drive rise, shrimp fry plant 10 and statistics pond 11 intercommunication, water in the shrimp fry plant 10 flows to statistics pond 11 in automatically, when making the first lifter plate 102 of third driving cylinder drive descend, shrimp fry plant 10 and statistics pond 11 do not communicate, water in the shrimp fry plant 10 stops to flow to statistics pond 11 in.
It should be understood that a first fixing member is provided above the shrimp farm 10, and a third driving cylinder is provided in the first fixing member.
Step S102: the weight W1 of the water in the counting tank 11 is weighed by a first electronic scale.
Preferably, the bottom surface of the counting chamber 11 is provided with a first electronic scale for weighing the water in the counting chamber 11, so that the weight of the water in the counting chamber 11 can be weighed by the first electronic scale.
It should be understood that the first electronic scale of this embodiment starts to weigh W1 the water in the statistical tank 11 only when it is detected that the water in the shrimp farm 10 stops flowing into the statistical tank 11.
Step S103: the cartridge 12 is lowered into the counting chamber 11 and the cartridge 12 is controlled to collect the water in the counting chamber 11, and after the cartridge 12 collects the water in the counting chamber 11, the cartridge 12 is controlled to ascend away from the water surface of the water in the counting chamber 11.
It should be appreciated that in step S103, the cartridge 12 collects the water in the statistical pool 11 containing shrimp larvae.
Step S104: the weight W2 of the water in the cartridge 12 is weighed using a second electronic scale.
Preferably, the bottom surface of the cartridge 12 is provided with a second electronic scale for weighing the water in the cartridge 12, so that the water in the cartridge 12 can be weighed by the second electronic scale.
It should be appreciated that the second electronic scale of this embodiment begins weighing the water W1 in the cartridge 12 only when the cartridge 12 is detected above the water level in the stat basin 11.
Step S105: a picture is taken with the camera 118 toward a side of the cartridge 12 to obtain a sample picture, and the cartridge 12 is then controlled to lower and drain the water from the cartridge 12.
It will be appreciated that in step S105, the water within the cartridge 12 is re-drained back into the stats basin 11 primarily so that the weight of the water within the stats basin 11 remains unchanged.
Step S106: and identifying the sampling number V1 of the shrimp larvae through the sampling pictures.
It should be understood that the present embodiment may transmit the taken sample picture to the background controller by the camera 118, so that the sample picture is processed by the background controller to identify the sample number V1 of the shrimp larvae in the sample picture.
Step S107: the cartridge 12 is controlled to move to the next collection point.
It will be appreciated that the control of movement of the cartridge 12 to the next collection point is primarily for the convenience of the cartridge 12 collecting water at different locations of the statistics pool 11.
Step S108: the steps S103-S106 are repeatedly executed to obtain a plurality of sampling numbers V1 of the shrimp larvae, and the shrimp larvae average value V is calculated through the plurality of sampling numbers V1.
Preferably, the cartridge 12 is lowered each time to the same weight of water collected in the statistics pool 11.
Step S109: the total number S of shrimp larvae in the statistical pond 11 was calculated by the formula S = W1/W2 x V.
It should be understood that the present embodiment calculates the total number S of shrimp larvae in the sampling box 12 by the formula S = W1/W2 × V, so that a large number (e.g., hundreds or thousands) of shrimp larvae can be counted, and the efficiency is higher.
In this embodiment, the first output port of the counting tank 11 is communicated with the recycling tank 13 through the second conveying pipeline 111, so that the water in the counting tank 11 can flow into the recycling tank 13.
Preferably, the horizontal plane of the bottom surface of the statistical pool 11 is higher than the horizontal plane of the bottom surface of the recovery pool 13, so that water in the statistical pool 11 can flow into the recovery pool 13 according to self gravity, manual operation is not needed, the work efficiency is high, and the labor cost is low.
In this embodiment, the first output port of the counting tank 11 is provided with a second switch mechanism, and when the second switch mechanism is turned on, the water in the counting tank 11 automatically flows into the recovery tank 13 through the second conveying pipeline 111. It should be understood that, because the bottom surface of statistics pond 11 is at the horizontal plane higher than the bottom surface of recovery pond 13, when second on-off mechanism opened, the water in statistics pond 11 because the gravity reason can flow to recovery pond 13 automatically through second pipeline 111 in, need not manual operation, directly realizes with the help of gravity that the shrimp seedling in statistics pond 11 is automatic to be carried to recovery pond 13 in, conveniently retrieves to shrimp seedling plant 10 or conveniently carries out other operations.
Step S110: and detecting and counting the activity of the shrimp seeds in the pond 11.
It should be understood that the main steps for detecting the activity of the shrimp larvae in the statistical pond 11 include: randomly extracting a plurality of shrimp seeds in the statistical pool 11, performing follow-up identification on the plurality of extracted shrimp seeds, obtaining a plurality of activity tracks of the plurality of shrimp seeds, judging whether the plurality of activity tracks (namely the lengths of the shrimp seeds moving in the preset walking time) meet preset track lengths in preset walking time, if the activity tracks of all the extracted shrimp seeds meet the preset track lengths, proving that the activity of the shrimp seeds in the statistical pool 11 meets the health requirement, and if the activity tracks of part or all the extracted shrimp seeds do not meet the preset track lengths, determining that the activity of the shrimp seeds in the statistical pool 11 does not meet the activity requirement.
Step S111: randomly extracting a plurality of shrimp larvae samples from the counting pool 11, putting the shrimp larvae samples into a collecting pipe containing an extraction solution, and uniformly dispersing and cracking.
Preferably, the extraction solution consists of the following components: tris buffer, 1M guanidinium isothiocyanate, 0.5wt% TritonX100, 1wt% Tween-20, 60. Mu.g/mL proteinase K, wherein the pH of the Tris buffer is 8.0.
Step S112: the adsorption membrane is immersed in the extraction solution.
Preferably, the adsorption membrane is a cellulose membrane treated with an anionic resin containing a quaternary ammonium salt.
Step S113: and (3) soaking and washing the adsorption film by using a washing buffer solution, and placing the adsorption film in an eluent or a PCR reaction solution to release nucleic acid.
Preferably, the washing buffer is Tris buffer containing 0.1wt% Tween-20.
Preferably, in step S113, the nucleic acid is released under conditions of pH = 8.3. It should be understood that, in steps S111-S113, a large number of quaternary ammonium bases are distributed on the surface of the cellulose membrane (i.e. the adsorption membrane) treated by the anion resin containing quaternary ammonium salt under the weak base condition of Tris buffer solution, and anion exchange is performed with nucleic acid in the sample, so as to rapidly adsorb the nucleic acid on the cellulose membrane, after washing and removing impurities, the nucleic acid is eluted and released, and further PCR amplification and the like are performed, so as to rapidly obtain genetic information of the sample.
It should be understood that, in the present embodiment, by randomly extracting a plurality of shrimp larvae samples in the counting pool 11, and performing nucleic acid acquisition, PCR amplification and the like is further performed conveniently, so as to obtain genetic information of the shrimp larvae samples quickly, and perform various detections conveniently, so that various detections can be performed on the shrimp larvae in the counting pool 11, including detections of fungi, bacteria, viruses and antibodies, so that whether viruses exist in the shrimp larvae collected in the counting pool 11 can be further detected. It should be noted that, since the shrimp fries collected by the statistical pond 11 are random, the nucleic acid extraction of the shrimp fries in the statistical pond 11 can be performed, and whether viruses exist or not detected by the nucleic acid can represent the shrimp fries in the whole statistical pond 11, and can represent the condition that the shrimp fries in the whole shrimp fry farm 10 are infected with the viruses, that is, the shrimp fries randomly grabbed by the statistical pond 11 are detected by the nucleic acid to be infected with the viruses, so that the shrimp fries in the whole shrimp fry farm 10 can be proved to have the virus infection.
It is worth noting that when the shrimp larvae in the counting pond 11 are determined to be not in the activity requirement, all the shrimp larvae in the counting pond 11 are conveyed to the nutrition pond, and it is understood that the nutrition pond contains nutrition, so that the shrimp larvae stored in the nutrition pond can be in the activity better and reach the health standard.
Further, the intelligent shrimp larvae detection method based on the bioinformatics technology further comprises the following steps:
after the total number S of the shrimp seeds in the sampling box 12 is calculated, the second switch mechanism is controlled to be opened, so that the water in the counting tank 11 automatically flows into the recovery tank through the second conveying pipeline 111.
In this embodiment, second on-off mechanism includes that the liftable sets up second lifter plate 112 in the first delivery outlet of statistics pond 11 and the fourth drive jar that is used for controlling second lifter plate 112 lift for when fourth drive jar drive second lifter plate 112 rose, statistics pond 11 and recovery pond 13 intercommunication, water in the statistics pond 11 flows to recovery pond 13 in automatically, when making fourth drive jar drive second lifter plate 112 descend, statistics pond 11 and recovery pond 13 do not communicate, water in the statistics pond 11 stops flowing to recovery pond 13 in.
It will be appreciated that a second mounting is provided above the counting chamber 11, in which a fourth drive cylinder is provided.
In this embodiment, the second output port of the counting pool 11 is communicated with the selling box 14 through the third conveying pipeline 113, so that the shrimp larvae in the counting pool 11 can flow to and sell the selling box 14, and the sale is realized.
Preferably, the horizontal plane of the bottom surface of the counting pool 11 is higher than the horizontal plane of the bottom surface of the selling box 14, so that water in the counting pool 11 can flow into the selling box 14 according to self gravity, manual operation is not needed, the working efficiency is high, and the labor cost is low.
In this embodiment, the second outlet of the counting tank 11 is provided with a third switch mechanism, and when the third switch mechanism is opened, the water in the counting tank 11 automatically flows into the selling box 14 through the third conveying pipeline 113. It should be understood that, because the horizontal plane of the bottom surface of the counting pool 11 is higher than the horizontal plane of the bottom surface of the selling box 14, when the third switching mechanism is opened, the water in the counting pool 11 will automatically flow into the selling box 14 through the third conveying pipeline 113 due to gravity, without manual operation, the shrimp seeds in the counting pool 11 are automatically conveyed into the selling box 14 directly by means of gravity, and the shrimp seeds are sold.
Further, the intelligent shrimp larvae detection method based on the bioinformatics technology further comprises the following steps:
step A1: after the total number S of the young shrimps in the sampling box 12 is calculated, whether the total number S of the young shrimps reaches the pre-sale number is judged.
Wherein, the pre-sale quantity is an artificial set value, namely when the shrimp larvae are required to be sold, the number of the shrimp larvae can be set in advance.
Step A2: and if the total number S of the young shrimps is determined to reach the pre-sale number, controlling the third switch mechanism to be opened so that the water in the counting pool 11 automatically flows into the sales box 14 through the third conveying pipeline 113.
Step A3: and if the total quantity S of the young shrimps is determined not to reach the pre-sale quantity, controlling the first switch mechanism to be opened so that the water in the young shrimp culturing farm 10 automatically flows into the counting tank 11 through the first conveying pipeline 101.
It will be appreciated that upon determining that the total number S of shrimp larvae has not reached the pre-sale number, the first switch mechanism is controlled to open, primarily to deliver more shrimp larvae into the counting chamber 11.
Step A4: steps S102-S108 are repeatedly performed so that the total number S of shrimp larvae reaches the pre-sale number.
In this embodiment, the third switch mechanism includes a third lifting plate 114 arranged in the second output port of the statistics pool 11 in a lifting manner and a fifth driving cylinder for controlling the third lifting plate 114 to lift, so that when the fifth driving cylinder drives the third lifting plate 114 to lift, the statistics pool 11 is communicated with the sales box 14, water in the statistics pool 11 automatically flows into the sales box 14, so that when the fifth driving cylinder drives the third lifting plate 114 to descend, the statistics pool 11 is not communicated with the sales box 14, and water in the statistics pool 11 stops flowing into the sales box 14.
It will be appreciated that a third mounting is provided above the counting chamber 11, in which a fifth driving cylinder is provided.
In this embodiment, a first screw 115, a first connecting member 1151 screwed into the first screw 115, and a first driving cylinder 116 fixedly disposed below the first connecting member 1151 are disposed above the counting chamber 11. Wherein the telescopic rod of the first driving cylinder 116 is arranged towards the counting pool 11, and the telescopic rod of the first driving cylinder 116 is fixedly connected with the sampling box 12, so as to control the lifting of the sampling box 12 through the first driving cylinder 116.
It should be understood that a motor is connected to the first lead screw 115 to control the first lead screw 115 to rotate through the motor. Preferably, the first screw 115 is disposed above the center of the statistic pool 11 along the length direction of the statistic pool 11.
Further, in step S106, the first screw 115 is controlled to rotate to control the movement of the cartridge 12 to the next collection point.
In this embodiment, the first screw 115 is further threadedly connected with a second connecting member 117, wherein the second connecting member 117 is connected with the first connecting member 1151 through a first connecting rod 1171, and the bottom of the second connecting member 117 is provided with a camera 118 for taking a picture of one side of the sample box 12. It should be understood that, since the second link 117 is connected to the first link 1151 by the first link 1171, the first link 1151 and the second link 117 are rotated in synchronization when the first lead screw 115 is rotated.
Preferably, one side of the cartridge 12 adjacent the camera 118 is transparent and the other side of the cartridge 12 remote from the camera 118 is opaque, which allows for a clear image of the shrimp larvae inside the cartridge 12.
In this embodiment, sampling box 12 is the rectangle form, wherein is equipped with the collection cavity in the sampling box 12, and the bottom of sampling box 12 is equipped with the collection mouth that communicates with the collection cavity, and the bottom liftable of sampling box 12 is provided with the sealed lid 121 that is used for sealed collection mouth.
Further, the shell of first driving cylinder 116 extends and is equipped with the mounting bracket, is provided with second driving cylinder 1161 in the mounting bracket, and wherein the telescopic link of second driving cylinder 1161 sets up towards statistics pond 11, and the telescopic link and the sealed lid 121 fixed connection of second driving cylinder 1161 to drive sealed lid 121 through second driving cylinder 1161 and go up and down.
Preferably, the sealing cap 121 is provided with an arc-shaped silicone sealing gasket 122 facing the top surface of the sampling cartridge 12, so as to effectively seal the collection port through the silicone sealing gasket 122.
Further, the intelligent shrimp larvae detection method based on the bioinformatics technology further comprises the following steps:
step B1: when the sampling box 12 descends and submerges on the water surface of the counting tank 11, the sealing cover 121 is driven to descend by the second driving cylinder so that the sealing cover 121 leaves the collecting opening, and after the sampling box 12 is soaked for a preset time, the sealing cover 121 is driven to ascend by the second driving cylinder so as to seal the collecting opening.
It should be appreciated that in step B1, the immersion of the cartridge 12 for the predetermined period of time is primarily intended to disperse the shrimp larvae evenly throughout the various positions (including within the cartridge 12 and within the statistical pond 11)
Step B1: after the sealing cap 121 seals the collection port, the first driving cylinder 116 drives the sampling box 12 to ascend.
In summary, the shrimp larvae are not required to be acquired manually, water of a shrimp larvae farm can be conveyed to the statistic pool in an automatic mode, the shrimp larvae are counted, labor cost is low, working efficiency is high, the shrimp larvae can be counted in a large batch, in addition, a plurality of shrimp larvae samples in the statistic pool are randomly extracted, nucleic acid acquisition is carried out, PCR amplification and the like are conveniently carried out, so that genetic information of the shrimp larvae samples is quickly acquired, and various detections are conveniently carried out.
The above description is only an embodiment of the present invention, and is not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes performed by the present invention or directly or indirectly applied to other related technical fields are included in the scope of the present invention.
Claims (10)
1. An intelligent shrimp larvae detection method based on a biological information technology is characterized by comprising the following steps:
step S101: conveying water in a shrimp fry farm to a statistical pond, wherein the shrimp fry farm is cultured with shrimp fries, the horizontal plane of the bottom surface of the shrimp fry farm is higher than the horizontal plane of the bottom surface of the statistical pond, an output port of the shrimp fry farm is communicated with an input port of the statistical pond through a first conveying pipeline, the output port of the shrimp fry farm is provided with a first switching mechanism, and when the first switching mechanism is opened, the water in the shrimp fry farm automatically flows into the statistical pond through the first conveying pipeline;
step S102: weighing the weight W1 of the water in the counting pool by using a first electronic scale, wherein the first electronic scale for weighing the weight of the water in the counting pool is arranged on the bottom surface of the counting pool;
step S103: lowering a cartridge into the statistics pool and controlling the cartridge to collect water within the statistics pool and, after the cartridge collects water within the statistics pool, controlling the cartridge to rise away from a surface of the water within the statistics pool;
step S104: weighing the weight W2 of the water in the sampling box by using a second electronic scale, wherein the bottom surface of the sampling box is provided with the second electronic scale for weighing the weight of the water in the sampling box;
step S105: taking a picture of one side of the sampling box by using a camera to obtain a sampling picture, and then controlling the sampling box to descend and discharge water in the sampling box;
step S106: identifying the sampling number V1 of the shrimp larvae through the sampling pictures;
step S107: controlling the cartridge to move to the next collection point;
step S108: repeatedly executing the steps S103-S106 to obtain a plurality of sampling numbers V1 of the shrimp larvae, and calculating the average value V of the shrimp larvae through the plurality of sampling numbers V1, wherein the weight of the collected water is the same when the sampling box descends to the statistic pool each time;
step S109: calculating the total number S of the shrimp seeds in the statistical pool through a formula S = W1/W2V;
step S110: detecting the liveness of the shrimp fries in the statistical pool;
step S111: randomly extracting a plurality of shrimp larvae samples from the statistic pool, putting the shrimp larvae samples into a collecting pipe containing an extraction solution, and uniformly dispersing and cracking, wherein the extraction solution comprises the following components: tris buffer, 1M guanidinium isothiocyanate, 0.5wt% TritonX100, 1wt% Tween-20, 60 μ g/mL proteinase K, said Tris buffer having a pH of 8.0;
step S112: dipping an adsorption film in the extraction solution, wherein the adsorption film is a cellulose film treated by quaternary ammonium salt-containing anion resin;
step S113: the adsorption membrane is washed with a washing buffer, which is Tris buffer containing 0.1wt% Tween-20, and placed in the eluent or PCR reaction solution to release the nucleic acids.
2. The intelligent shrimp larvae detection method based on bioinformatics technology as claimed in claim 1, wherein in step S113, nucleic acid is released under the condition of pH =8.3, the first output port of the statistical tank is communicated with the recovery tank through a second delivery pipe, the bottom surface of the statistical tank is located at a higher level than the bottom surface of the recovery tank, the first output port of the statistical tank is provided with a second switch mechanism, and when the second switch mechanism is turned on, water in the statistical tank automatically flows into the recovery tank through the second delivery pipe, and the method further comprises:
and after the total quantity S of the shrimp seeds in the sampling box is calculated, controlling the second switch mechanism to be switched on so that the water in the counting tank automatically flows into the recovery tank through a second conveying pipeline.
3. The intelligent shrimp postlarvae detection method based on bioinformatics technology as claimed in claim 2, wherein the second outlet of the statistical tank is communicated with the sales box through a third delivery pipe, the horizontal plane of the bottom surface of the statistical tank is higher than the horizontal plane of the bottom surface of the sales box, the second outlet of the statistical tank is provided with a third switching mechanism, when the third switching mechanism is opened, the water in the statistical tank automatically flows into the sales box through the third delivery pipe, and the method further comprises:
after the total number S of the shrimp seeds in the sampling box is calculated, judging whether the total number S of the shrimp seeds reaches a pre-sale number, wherein the pre-sale number is an artificial set value;
and if the total quantity S of the shrimp seeds is determined to reach the pre-sale quantity, controlling the third switch mechanism to be opened so that the water in the counting pool automatically flows into the sales box through a third conveying pipeline.
4. An intelligent shrimp larvae detection method based on bioinformatics technology according to claim 3, wherein the method further comprises:
if the total quantity S of the shrimp seeds is determined not to reach the pre-sale quantity, controlling the first switch mechanism to be opened so that the water in the shrimp seed farm automatically flows into the statistical pool through a first conveying pipeline;
the steps S102 to S108 are repeatedly performed so that the total number S of shrimp larvae reaches the pre-sale number.
5. The intelligent shrimp larvae detecting method based on bioinformatics technology as claimed in claim 4, wherein a first lead screw, a first connecting piece screwed into the first lead screw and a first driving cylinder fixedly arranged below the first connecting piece are arranged above the counting pool, wherein a telescopic rod of the first driving cylinder is arranged towards the counting pool, and the telescopic rod of the first driving cylinder is fixedly connected with the sampling box so as to control the lifting of the sampling box through the first driving cylinder.
6. The intelligent shrimp postlarvae detection method based on bioinformatic technology as claimed in claim 5, wherein a second connecting piece is further in threaded connection with the first screw mandrel, wherein the second connecting piece is connected with the first connecting piece through a first connecting rod, and a camera for taking a picture towards one side of the sampling box is arranged at the bottom of the second connecting piece.
7. The intelligent shrimp larvae detection method based on bioinformation technology as claimed in claim 6, wherein the sampling box is rectangular, a collection cavity is arranged in the sampling box, a collection port communicated with the collection cavity is arranged at the bottom of the sampling box, a sealing cover used for sealing the collection port is arranged at the bottom of the sampling box in a lifting manner, one side of the sampling box close to the camera is transparent, and the other side of the sampling box far away from the camera is opaque.
8. The intelligent shrimp larvae detection method based on bioinformation technology as claimed in claim 7, wherein the housing of the first driving cylinder is extended to form a mounting rack, the mounting rack is provided with a second driving cylinder, the telescopic rod of the second driving cylinder is arranged towards the statistics pool, the telescopic rod of the second driving cylinder is fixedly connected with the sealing cover so as to drive the sealing cover to lift through the second driving cylinder, and the top surface of the sealing cover towards the sampling box is provided with an arc-shaped sealing silica gel pad.
9. The intelligent shrimp larvae detecting method based on the bioinformatic technology as claimed in claim 8, further comprising:
when the sampling box descends and submerges on the water surface of the counting pool, the second driving cylinder drives the sealing cover to descend so that the sealing cover leaves the collecting opening, and after the sampling box is soaked for a preset time, the second driving cylinder drives the sealing cover to ascend so as to seal the collecting opening.
10. The intelligent shrimp larvae detecting method based on bioinformatics technology as claimed in claim 9, wherein the first switch mechanism comprises a first lifting plate arranged in an output port of the shrimp larvae farm in a lifting manner and a third driving cylinder for controlling the lifting of the first lifting plate, so that when the third driving cylinder drives the first lifting plate to ascend, water in the shrimp larvae farm automatically flows into the statistical pool, and when the third driving cylinder drives the first lifting plate to descend, the water in the shrimp larvae farm stops flowing into the statistical pool;
the second switch mechanism comprises a second lifting plate arranged in a first output port of the statistical pool in a lifting mode and a fourth driving cylinder used for controlling the second lifting plate to lift, so that when the fourth driving cylinder drives the second lifting plate to lift, water in the statistical pool automatically flows into the recovery pool, and when the fourth driving cylinder drives the second lifting plate to lower, the water in the statistical pool stops flowing into the recovery pool;
the third switch mechanism comprises a third lifting plate arranged in an output port of the shrimp fry farm in a lifting mode and a fifth driving cylinder used for controlling the third lifting plate to lift, so that when the fifth driving cylinder drives the third lifting plate to lift, water in the counting tank automatically flows into the selling box, and when the fifth driving cylinder drives the third lifting plate to fall, the water in the counting tank stops flowing into the selling box.
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| US20210289759A1 (en) * | 2016-12-15 | 2021-09-23 | University Of The Philippines | Estimating fish size, population density, species distribution and biomass |
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| CN101710391A (en) * | 2009-12-14 | 2010-05-19 | 张康德 | Cellular fish (shrimp) seedling counting device |
| US20210289759A1 (en) * | 2016-12-15 | 2021-09-23 | University Of The Philippines | Estimating fish size, population density, species distribution and biomass |
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