CN115615792A - Device for filtering micro-samples - Google Patents
Device for filtering micro-samples Download PDFInfo
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- CN115615792A CN115615792A CN202211371615.6A CN202211371615A CN115615792A CN 115615792 A CN115615792 A CN 115615792A CN 202211371615 A CN202211371615 A CN 202211371615A CN 115615792 A CN115615792 A CN 115615792A
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- China
- Prior art keywords
- filtering
- filter
- micro
- sample
- adapter
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- Pending
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- 238000001914 filtration Methods 0.000 title claims abstract description 58
- 238000005245 sintering Methods 0.000 claims abstract description 5
- 239000004699 Ultra-high molecular weight polyethylene Substances 0.000 claims description 13
- 229920000785 ultra high molecular weight polyethylene Polymers 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 10
- 239000004698 Polyethylene Substances 0.000 claims description 5
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- -1 polyethylene Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 238000005406 washing Methods 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 238000004090 dissolution Methods 0.000 abstract description 3
- 238000005260 corrosion Methods 0.000 abstract description 2
- 230000007797 corrosion Effects 0.000 abstract description 2
- 238000013461 design Methods 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 28
- 239000000243 solution Substances 0.000 description 15
- 238000010586 diagram Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 239000004677 Nylon Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229920006351 engineering plastic Polymers 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/11—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
Abstract
The invention discloses a device for filtering a micro-sample, which comprises a supporting structure, an adapter, a filtering column and a sample tube, wherein the adapter is connected with the supporting structure; wherein the bottom of the filtering column is provided with a sieve plate; adapter open-top can connect the pressurized equipment, the adapter bottom with filter the post and meet, bearing structure is used for fixing filter the post, the sample cell is located filter under the post, it is provided with the sieve of being made by the PE sintering to filter the toe portion, the sieve is narrow shape under wide. The device for filtering the micro-samples provided by the invention designs the universal filter column to meet the requirements of better compatibility and corrosion resistance of most solutions in the process of filtering, and can reduce the dissolution of filter media caused by misusing different materials. The device for filtering the micro-sample provided by the invention can also reduce the solution residue to the maximum extent, and then achieve the highest recovery rate through micro-washing, especially under the condition that the sample amount is small and the sample is precious.
Description
Technical Field
The invention belongs to the technical field of filtering devices, and particularly relates to a device for filtering a micro-sample
Background
With the rapid development of biotechnology, detection techniques for liquid-phase purification of macromolecular biological products are becoming more and more popular. The method comprises the following steps of (1) generally pretreating a biological sample needing liquid phase detection before sample introduction; among them, filtration is a key pretreatment step. Under the general condition, can select the filtration membrane or the filter in different apertures to filter according to the characteristic and the size of sample and reach the effect of preliminary treatment, its liquid phase testing process of sample after filtration treatment just can go on smoothly, thereby be unlikely to take place to block up and influence the experimental result, can also prolong the life of instrument consumptive material simultaneously.
The needle filter is used in most cases for filtering small samples of solution on the market today. Although the needle filter has a relatively large sample volume (100-1000 ul or more), it has a series of disadvantages: (1) The residual quantity of the solution is large, and the loss is large when the value of a sample is high; (2) If the final total volume is larger due to the washing, concentration treatment is usually required, which not only increases the operation steps, but also brings degradation risk; (3) When the concentration of the sample is thick, the needle head filter is easy to bounce and fall off due to overhigh pressure, so that the sample is splashed and lost; (4) When the sample amount is small (less than 50 ul), the cavity of the filter cannot be filled with liquid by using the needle filter, so that the sample is difficult to filter; (5) Solution samples of different systems usually need to be filtered by using syringe filters of different materials, and are easily mixed carelessly during the operation. In addition, aqueous filtration membranes can also be used to filter solution samples, such as: the common Mixed Cellulose (MCE) film has smooth surface, light and thin texture and high porosity; but also has the advantages of good interception effect, good hydrophilicity, large water flux, uniform microporous structure, extremely low adsorption rate and the like; however, MCE membranes are not resistant to organic solutions and strong acid and base solutions, and are only resistant to filtration at pH values of 2-9, and are therefore only suitable for filtration of aqueous drugs or other aqueous solvents. The aqueous membrane is also very likely to cause membrane dissolution and thus contamination of the sample when the sample containing the organic solvent is filtered. Therefore, it is necessary to develop a device for filtering a micro-sample with high efficiency and easy operation.
Disclosure of Invention
The invention aims to provide a device for filtering a micro-sample.
In order to achieve the above purpose, the solution of the invention is:
a device for filtering micro-samples, the device comprising a support structure, an adapter, a filter column and a sample tube; wherein the bottom of the filtering column is provided with a sieve plate; adapter open-top can connect the pressurized equipment, the adapter bottom with filter the post and meet, bearing structure is used for fixing filter the post, the sample cell is located filter under the post.
Preferably, the support structure is made of ultra high molecular weight polyethylene.
Preferably, the adapter is made of silica gel, and the size of the inner diameter is just consistent with the outer diameter of the filter column.
Preferably, the filter column is made of ultra-high molecular weight polyethylene.
Preferably, the sample tube is made of ultra-high molecular weight polyethylene.
Preferably, the sieve plate is made of polyethylene after sintering.
Preferably, the pressurizing device is a syringe.
Preferably, the filter column main part is frustum cone shape, and the diameter of going up the bottom surface is 10mm, and the diameter of lower bottom surface is 2.6mm, and the distance between upper and lower bottom surface is 23.4mm, and the vertical downwardly extending of lower bottom surface becomes 1 mm's pipeline.
Preferably, the sieve plate may also be covered with a PTFE membrane.
Compared with the existing device for filtering the sample, the principle and the gain effect of the invention are as follows:
1. the bottom of the filter column of the device for filtering the micro-samples is provided with a sieve plate made by sintering PE or a PE sieve plate covered by a PTFE membrane, and the shape of the sieve plate must be attached to the bottom of the filter column; therefore, the sieve plate is wide at the upper part and narrow at the lower part, and the lower end of the sieve plate is vertically extended downwards to form a pipeline; the benefit of this design is: on one hand, the solution can be reduced to form drops and be attached to the pipe wall to be remained, on the other hand, the lower interface of the filter column is reduced, so that the originally dropped liquid is columnar and is convenient to blow out, thereby reducing the residue, and finally, a small amount of liquid is used for rinsing, so that the minimum loss can be achieved. In addition, sieve plates with different pore diameters and whether a PTFE membrane needs to be covered on the sieve plates can be selected according to specific requirements in the operation process.
2. The device for filtering the micro-samples provided by the invention has the advantages that the universal filter column is designed to meet the filtering requirement of most solutions, the designed filter device has better compatibility and corrosion resistance, the use of materials is not required to be distinguished like a common needle filter, and the dissolution of a filter medium caused by misusing different materials can be reduced.
3. The device for filtering the micro-sample is mainly made of ultra-high molecular weight polyethylene (UHMW-PE), and the UHMW-PE has excellent impact resistance, abrasion resistance, low temperature resistance and self-lubricating property, and also has the following excellent properties: (1) Excellent resistance to chemical attack-in addition to strong oxidizing acids, to various aggressive media (acids, bases, salts) and organic media (except naphthalene solvents); (2) The functional component is sanitary, nontoxic, physiologically inert and physiologically adaptive, meets the standards of the Japan health Association, is approved by the American FDA and agricultural department, can be directly contacted with food and medicines, and can be implanted into human bodies; (3) excellent hydrophobicity: the water absorption of UHMW-PE is extremely low, less than 0.01 percent, only 1 percent of nylon, and belongs to one of the engineering plastics with the minimum water absorption.
4. The device for filtering the micro-sample provided by the invention can reduce the solution residue to the maximum extent, and then achieves the highest recovery rate through micro-washing, especially under the conditions of small sample amount and precious property.
Drawings
FIG. 1 is a schematic diagram of the structure of a device for filtering micro-samples; in the drawings: 1. the device comprises a supporting structure, 2. An adapter, 3. A filter column, 4. A sample tube and 5. A sieve plate.
FIG. 2 is a schematic cross-sectional view of a device for filtering micro-samples.
FIG. 3 is a schematic diagram of an adapter of the device for filtering micro-samples.
FIG. 4 is a schematic support diagram of a device for filtering micro-samples.
Detailed Description
The present invention will be described in further detail with reference to the following drawings and examples. It is also to be understood that the following examples are intended to illustrate the present invention and are not to be construed as limiting the scope of the invention, and that the particular materials, reaction times and temperatures, process parameters, etc. listed in the examples are exemplary only and are intended to be exemplary of suitable ranges, and that insubstantial modifications and adaptations of the invention by those skilled in the art in light of the foregoing description are intended to be within the scope of the invention.
The filtration sample used in the embodiment of the invention is a crude nucleotide solution, and the preparation method comprises the following steps: the crude oligonucleotide was dissolved in 1500ul of TE buffer and mixed well, and the concentration of the oligonucleotide was 488.6ng/ul.
The filtration yield calculation formula is as follows:
yield = V total volume post filtration × C post filtration concentration/(V pre filtration volume × C original concentration) × 100%;
wherein: volume before V filtration =200ul/50ul, original concentration of c =488.6ng/ul.
Example 1
Fig. 1 is a schematic structural diagram of an apparatus for filtering a micro sample according to the present invention, where the apparatus includes a support structure, an adapter, a filter column, and a sample tube; wherein the bottom of the filtering column is provided with a sieve plate; adapter open-top can connect the pressurized equipment, the adapter bottom with filter the post and meet, bearing structure is used for fixing filter the post, the sample cell is located filter under the post.
The support structure is made of ultra-high molecular weight polyethylene; fig. 4 is a schematic view of the support structure.
The adapter is made of silica gel, and the size of the inner diameter of the adapter is just consistent with the outer diameter of the filter column; fig. 3 is a schematic diagram of the adapter.
The filter column is made of ultra-high molecular weight polyethylene.
The sample tube is made of ultra-high molecular weight polyethylene.
The sieve plate is made of polyethylene after sintering.
The pressurizing device is a syringe.
The schematic diagram of the filter column is shown in fig. 2: the main part is frustum cone shape, and it is 10mm to go up the bottom surface diameter, and lower bottom surface diameter is 2.6mm, and the distance between upper and lower bottom surface is 23.4mm, and the vertical downwardly extending of lower bottom surface becomes 1 mm's pipeline.
The screen plate may also be covered with a PTFE membrane.
Example 2
The solution of the crude oligonucleotide (200. Mu.l or 50. Mu.l) was pipetted into the filtration column of the device for filtering a micro sample provided in the present invention, and pressurized using a 5ml syringe as a positive pressure. The syringe was pushed slowly to allow the filtrate to flow out. After filtering, adding 10-20ul of washing liquid for cleaning and washing, and pressurizing and filtering again; if a small amount of liquid remains in the subsequent process, the liquid can be completely blown out by adopting a syringe blowing mode, the operation is repeated for three times to obtain an average value, and the filtering result is shown in table 1.
Comparative example 1
Sucking the oligonucleotide crude product solution (200 ul or 50 ul), adding into a 5ml disposable needle syringe, filtering with a common nylon needle filter (diameter 13 mm), and adding 10-20ul washing solution for washing after filtering; the filtration results are shown in Table 1.
TABLE 1 filtration yield Table
As can be seen from the results of table 1: the average yield of the oligonucleotide crude product solution filtered by using the device for filtering the micro-samples is better than that of a disposable syringe needle matched with a common nylon needle filter, especially for micro-samples (such as 50 ul).
Claims (9)
1. A device for filtering micro-samples, characterized in that it comprises a support structure (1), an adapter (2), a filter column (3) and a sample tube (4); wherein, the bottom of the filtering column is provided with a sieve plate (5); adapter open-top can connect the pressurized equipment, the adapter bottom with filter the post and meet, bearing structure is used for fixing filter the post, the sample cell is located filter under the post.
2. A device for filtering micro-samples according to claim 1, characterized in that: the support structure is made of ultra-high molecular weight polyethylene.
3. A device for filtering micro-samples according to claim 1, characterized in that: the adapter is made of silica gel.
4. Device for filtering micro-samples according to claim 1, characterized in that: the filter column is made of ultra-high molecular weight polyethylene.
5. Device for filtering micro-samples according to claim 1, characterized in that: the sample tube is made of ultra-high molecular weight polyethylene.
6. A device for filtering micro-samples according to claim 1, characterized in that: the sieve plate is made of polyethylene after sintering.
7. A device for filtering micro-samples according to claim 1, characterized in that: the pressurizing device is a syringe.
8. A device for filtering micro-samples according to claim 1, characterized in that: the filter column main part is frustum cone shape, and it is 10mm to go up the bottom surface diameter, and lower bottom surface diameter is 2.6mm, and the distance between upper and lower bottom surface is 23.4mm, and the vertical downwardly extending of lower bottom surface becomes 1 mm's pipeline.
9. A device for filtering micro-samples according to claim 1, characterized in that: the sieve plate can also be covered with a PTFE membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211371615.6A CN115615792A (en) | 2022-11-03 | 2022-11-03 | Device for filtering micro-samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211371615.6A CN115615792A (en) | 2022-11-03 | 2022-11-03 | Device for filtering micro-samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115615792A true CN115615792A (en) | 2023-01-17 |
Family
ID=84877431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211371615.6A Pending CN115615792A (en) | 2022-11-03 | 2022-11-03 | Device for filtering micro-samples |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115615792A (en) |
-
2022
- 2022-11-03 CN CN202211371615.6A patent/CN115615792A/en active Pending
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