CN115606691A - Application of biotin as cow feed additive - Google Patents
Application of biotin as cow feed additive Download PDFInfo
- Publication number
- CN115606691A CN115606691A CN202211635890.4A CN202211635890A CN115606691A CN 115606691 A CN115606691 A CN 115606691A CN 202211635890 A CN202211635890 A CN 202211635890A CN 115606691 A CN115606691 A CN 115606691A
- Authority
- CN
- China
- Prior art keywords
- biotin
- milk
- total
- iso
- anteiso
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses an application of biotin in improving the content of functional fatty acid in milk. In-vitro and in-vivo experiments show that the addition of biotin to the basic ration of dairy cows can obviously improve the milk contentiso‑C15:0、iso‑C16:0、iso‑C17:0、anteiso‑C15:0、anteiso-C17:0, totalISOGeneral, aAnteisoTotal BCFA and total OBCFA content in milkisothe-C14: 0, C17:0, C23:0 and total OCFA content have a tendency to increase due to the addition of biotin. Therefore, the method determines that the content of total odd-chain fatty acids and branched-chain fatty acids in the milk can be effectively improved by adding the biotin into the basic daily ration of the milk cow, and accordingly, the method has a certain application prospect in the aspect of improving the quality of the milk.
Description
Technical Field
The invention relates to an application of biotin as a feed additive for dairy cows, in particular to an application of biotin as a feed additive in improving the contents of odd-numbered carbon chains and branched-chain fatty acids in milk, belonging to the field of new application of biotin as a feed additive.
Background
Odd-carbon chain and branched-chain fatty acids (OBCFA) are an important class of functional fatty acids in ruminant milk, which are primarily derived from the synthesis of rumen microorganisms. OBCFA includes odd-numbered chain fatty acids (e.g., C15: 0) and branched chain fatty acids, wherein the branched chain fatty acids are classified into isomeric branched chain fatty acids (iso-C14: 0, iso-C15: 0) and pre-isomeric branched chain fatty acids (anteiso-C15: 0, anteiso-C17: 0) due to differences in branching positions (Rong-Bull. Advance in the study of odd-numbered and branched chain fatty acids in milk fat. Feed industry. 2008 Vol. 29, no. 6).
Biotin, also known as vitamin H, is a low molecular weight organic compound essential for maintaining normal functions in animal bodies, and is used as a component of enzyme to participate in the metabolism of three major nutrients in the bodies. The addition of a certain amount of biotin to the daily ration of the dairy cow can increase the milk yield of the dairy cow and improve the reproductive performance of the dairy cow, but whether the addition of a certain amount of biotin to the daily ration of the dairy cow can increase the content of functional fatty acid in the milk is unknown and needs further research.
Disclosure of Invention
The invention mainly aims to provide the application of biotin in improving the content of functional fatty acid in milk.
According to the invention, biotin is added into the basic ration of the dairy cow, and in-vitro rumen fermentation and in-vivo tests show that the contents of odd-chain fatty acids and branched-chain fatty acids in milk are improved after the dairy cow is supplemented with the biotin; thus, the invention provides the use of biotin for increasing the content of odd and branched chain fatty acids in milk, comprising adding biotin as a feed additive to the basal ration of a cow to feed the cow.
Specifically, the invention discovers that the content (P < 0.05) of ISO-C15:0, ISO-C16:0, ISO-C17:0, anteiso-C15:0, anteiso-C17:0, total ISO, total Anteiso, total BCFA and total OBCFA in milk can be remarkably increased by adding biotin into daily ration of dairy cows, and the content (P < 0.1) of ISO-C14:0, C17:0, C23:0 and total OCFA in milk is increased by adding biotin. Therefore, the addition of biotin in the daily ration of the dairy cow effectively improves the content of total odd-chain fatty acids and branched-chain fatty acids in the milk, wherein the total OCFA, the total BCFA and the total OBCFA are respectively improved by 12.5 percent, 17.2 percent and 15.9 percent.
In a preferred embodiment of the present invention, the addition amount of biotin is 20 to 80 mg/calf/day, and more preferably, the addition amount of biotin is 40 to 80 mg/calf/day.
Detailed description of the invention
The invention combines an internal and external test to test whether the synthesis of odd-number carbon chains and branched-chain fatty acids is promoted after the biotin supplementation.
The invention first adds biotin at levels of 0, 1, 2 and 4mg/kg dry matter ration during in vitro fermentation, 5 replicates per level were set and the experiments were repeated within two weeks. The test result shows that the concentration of acetic acid, propionic acid, acetic acid/propionic acid and total volatile acid in the fermentation liquor is changed twice (P < 0.05) due to the addition of biotin, the concentration of isobutyric acid and butyric acid is linearly increased (P < 0.05) along with the increase of the addition level of biotin, and the concentration of isovaleric acid and total volatile acid in the fermentation liquor has the tendency of being linearly increased (P < 0.1). The concentrations of ISO-C13:0, ISO-C16:0, ISO-C17:0, anteiso-C15:0, anteiso-C17:0, C15:0, C17:0, total ISO, total Anteiso, total BCFA, total OCFA and total OBCFA in the fermentation liquor are remarkably and linearly increased along with the increase of the addition amount of biotin (P < 0.05), and C21:0 in the fermentation liquor has the trend of linear increase (P < 0.1); in addition, the concentrations of iso-C13:0, iso-C16:0, anteiso-C15:0, total Anteiso and total OCFA in the fermentation liquor simultaneously show obvious secondary changes (P < 0.05), and the concentrations of iso-C17:0, anteiso-C17:0, total BCFA and total OBCFA in the fermentation liquor have the trend of secondary changes (P < 0.1). Thus, in vitro fermentation experiments have found that supplementation with biotin at levels of 2mg/kg or 4mg/kg dry matter ration is effective in promoting the synthesis of odd and branched chain fatty acids.
On the basis of in vitro fermentation test, the invention further develops animal test. Selecting 20 Holstein cows, randomly dividing into two treatment groups, feeding a basic ration to a control group, supplementing biotin to the test group according to the level of 4mg/kg dry matter ration, and converting all cows according to the feed intake of 20kg dry matter, thereby determining that each cow needs to be supplemented with 80mg of biotin every day. The pre-feeding period is 14 days, the test period is 28 days, the feed intake and milk production are recorded every day during the test period, and rumen fluid and milk samples are collected at the end of the test. Animal test results show that the daily supplement of 80mg of biotin to dairy cows has no significant influence on the production performance and rumen fermentation, the contents of ISO-C15:0, ISO-C16:0, ISO-C17:0, anteiso-C15:0, anteiso-C17:0, total ISO, total Anteiso, total BCFA and total OBCFA in milk (P < 0.05), and the contents of ISO-C14:0, C17:0, C23:0 and total OCFA in milk have a tendency to increase due to the addition of biotin (P < 0.1). Therefore, animal experiments confirm that the addition of biotin to the daily ration of dairy cows can effectively increase the content of total odd-chain and branched-chain fatty acids in milk, wherein the total OCFA, the total BCFA and the total OBCFA are respectively increased by 12.5%, 17.9% and 16.3%.
Detailed Description
The invention is further described below in conjunction with specific embodiments, the advantages and features of which will become apparent from the description.
Test example 1 in vitro method for investigating the influence of biotin on the synthesis of odd-numbered and branched-chain fatty acids in rumen fermentation broth
1. Materials and methods
1.1 Rumen fluid Collection
3 Holstein cows which are healthy, have similar weight and lactation age and are provided with a permanent rumen fistula are selected as rumen fluid donors in the Changping animal test base of the Beijing animal husbandry and veterinary institute of Chinese academy of agricultural sciences. The donor cow diets were formulated according to NRC (2001) to meet all nutritional requirements (table 1). Feeding milk cow with water for 2 times per day, collecting rumen content via rumen fistula 1 hr before feeding, mixing, placing in a vacuum flask, rapidly returning to laboratory, and filtering with 4 layers of gauze (introducing CO simultaneously) 2 ) The whole operation is carried out in a water bath at 39 ℃.
TABLE 1 Donor Dairy cow diet composition and Nutrition level (Dry matter basis)
1.2 Buffer solution preparation
The buffer solution is prepared by a method of reference king plus starter (2011), which specifically comprises the following steps:
(1) Solution A:13.2 g/dL CaCl 2 2H 2 O，10 g/dL MnCl 2 4H 2 O，1.0 g/dL CoCl 2 •6H 2 O，8.0 g/dL FeCl 3 6H 2 O。
(2) Solution B:0.4 g/dL NH 4 HCO 3 ，3.5 g/dL NaHCO 3 。
(3) Solution C:0.57 g/dL Na 2 HPO 4 ，0.62 g/dL K 2 HPO 4 ，0.06 g/dL MgSO 4 • 7H 2 O。
(4) Reducing agent: 0.160 mg/dL NaOH,0.625 g/dL Na 2 S • 9H 2 O。
(5) Resazurin solution: 100mg of resazurin was dissolved in 100mL of distilled water.
Before the test, rumen buffer solution is prepared according to the following proportion: adding 0.1mL of solution A, 200mL of solution B, 200mL of solution C, 1mL of resazurin solution and 40mL of reducing agent solution (prepared temporarily when used) into 400mL of distilled water, mixing the above solutions, introducing CO 2 Until saturation, the pH value is 6.8 to 7.0, and the mixture is preheated to 39 ℃.
1.3 Design of experiments
In the experiment, biotin is added according to 0, 1, 2 and 4mg/kg dry matter, 20 fermentation bottles are randomly divided into 4 treatment groups in the experiment, 5 repetitions are set for each treatment group, 0.5g of total mixed ration (shown in table 2) is added into each fermentation bottle, 25mL of rumen fluid and 50mL of buffer fluid are added and mixed uniformly, and CO is introduced at the same time 2 Replacing oxygen, sealing the rubber plug, placing the rubber plug in a gas bath constant temperature shaking table, and culturing for 24 hours at the constant temperature of 39 ℃. This experiment was repeated twice in two weeks.
TABLE 2 fermentation substrate Total Mixed diet composition and Nutrition level (Dry matter basis)
2. Sample collection and measurement
2.1 Determination of pH value and dry matter degradation rate of rumen fermentation liquor
After 24h fermentation, the fermentation broth was decanted, the pH was measured immediately using a pH meter calibrated with standard solutions, and the pH was recorded after the readings were stable. And (3) filtering the fermentation liquor after the pH is measured by using a nylon bag, putting the residues and the nylon bag into a 65 ℃ oven to be dried to constant weight for measuring the degradation rate of the dry matter, subpackaging the filtered fermentation liquor, and storing at-20 ℃ for later use.
2.2 Determination of concentration of volatile acid in rumen fermentation broth
2.2.1 Sample pretreatment
Volatile Fatty Acid (VFA) in fermentation broth was determined by the method of the references (wang gakai. Ruminant nutrition research methods [ M ]. Modern education press, 2011.) as follows: centrifuging 5mL of filtered fermentation liquor at 4 ℃ at 10000 Xg for 10min, transferring 1mL of supernate into a centrifuge tube, adding 0.1mL of 25% metaphosphoric acid, standing for 30min in ice bath, centrifuging at 4 ℃ at 10000 Xg for 15min, detecting the supernate by using a gas chromatograph, and measuring the concentrations of acetic acid, propionic acid, butyric acid, isobutyric acid, isovaleric acid, valeric acid, total Volatile Fatty Acid (TVFA) and total branched-chain volatile fatty acid (TBVFA) by an external standard method.
2.2.2 Instrumental detection
Total volatile fatty acid detection was determined using an Agilent DB-FFAP chromatography column (15 m.times.0.25 mm.times.0.25 μm) in a constant pressure mode of 50Kpa, with a column oven starting temperature of 70 deg.C, then ramping up to 125 deg.C at 3 deg.C/min, then ramping up to 180 deg.C at 30 deg.C/min and maintaining for 5min, with high purity nitrogen as carrier gas, split ratio of 1.
2.3 Determination of fatty acid in rumen fermentation broth
Determination of fatty acids in fermentation broths was determined with appropriate modification based on the method of the references (wang gakai. Ruminant nutrition research methods [ M ]. Modern education press, 2011.).
2.3.1 Reagents (unless otherwise stated, all reagents used in this test were analytically pure and primary water as specified in GB/T6682)
(1) Anhydrous sodium sulfate (Na) 2 SO 4 ）
(2) N-hexane [ CH 3 (CH 2 ) 4 CH 3 ]: pure chromatography
(3) Isopropanol [ (CH) 3 ) 2 CHOH]
(4) 2% sodium hydroxide in methanol: 2.0g of NaOH is weighed and dissolved in 100mL of anhydrous methanol, and the mixture is mixed evenly and prepared on site.
(5) 10% hydrochloric acid methanol solution: 10mL of Chloroacetyl (CH) was taken 3 COCl) is slowly injected into 100mL of anhydrous methanol and mixed evenly, and the mixture is prepared just before use.
Note that: when the chloroacetyl is poured into methanol, care is taken to prevent splattering and the process is carried out in a fume hood.
(6) 66.7g/L sodium sulfate solution: 6.67g of anhydrous sodium sulfate was weighed out and dissolved in 100mL of water.
(7) N-hexane-isopropyl alcohol mixture: 3 volumes of n-hexane and 2 volumes of isopropanol were mixed well.
(8) Fatty acid standard: 37 standards of fatty acid methyl esters (Supelco 37 Component FAME Mix) were purchased from Simga (Sigma-Aldrich, st. Louis, mo.). Standards for iso-C13:0, anteiso-C13:0, iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:0, iso-C17:0, and anteiso-C17:0 are available from LarodaNB (Karolinska Institute Science Park, solna, sweden).
2.3.2 Sample pretreatment
Taking a fermentation liquid sample from 3mL to 10mL, adding 5mL of n-hexane-isopropanol mixed solution, and carrying out vortex oscillation for 2min. 2mL of sodium sulfate solution was added, vortexed and shaken for 2min, and centrifuged at 5000 Xg for 10min at 4 ℃. Transferring the upper n-hexane phase into a high-temperature-resistant test tube, re-extracting and centrifuging the emulsion layer which cannot be separated in the middle and the lower solution, combining all the n-hexane phases into the high-temperature-resistant test tube, and drying by using nitrogen. Then 0.5mL of n-hexane and 1mL of methanol and 2mL of methanolic sodium hydroxide solution are added, the tube lid is screwed down, shaken well and saponified in a water bath at 50 ℃ for 30min. After cooling to room temperature, 2mL of methanol hydrochloride solution was added and esterified in a water bath at 90 ℃ for 2h. Cooling to room temperature, adding 3mL of water and 5mL of n-hexane, oscillating, standing for layering, taking the upper layer liquid, blowing nitrogen to be nearly dry, adding 0.5mL of n-hexane, uniformly mixing by vortex for 30s, adding about 0.1g of anhydrous Na2SO4, drying, and performing on-machine determination.
2.3.3 Instrumental determination
Fatty acid detection was performed using an agilent HP-88 chromatography column (100 m x 250 μm x 0.20 μm) in a constant pressure mode of 190Kpa with a column oven start temperature of 120 ℃ and maintained for 10min, followed by 1.5 ℃/min ramp up to 230 ℃ and maintained for 30min, with high purity nitrogen as carrier gas, split ratio of 1.
3. Statistical analysis
The pH of the fermentation broth, the dry matter degradation rate, the concentration of volatile acids and the content of branched chain fatty acids were statistically examined using a PROC MIXED module in SAS software (Version 9.4, SAS Institute Inc., cary, NC), and the statistical model included the random effect of the fermentation batch and the fixed effect of the experimental treatment. The significance of the linear and quadratic interval was examined for a polynomial matrix for processing (multinomial const). The statistical results of the variables are all expressed in the form of least square mean, the significance level is P <0.05, and the significance trend level is more than or equal to 0.05 and less than or equal to P <0.1.
4. Test results
4.1 Effect of daily ration addition of biotin on rumen fermentation parameters
The effect of different levels of biotin added to the diets on rumen fermentation parameters is shown in table 3. The test results show that the biotin addition has no significant influence on the degradation rate of dry matters of daily ration and the pH value of the fermentation liquor, but the concentrations of acetic acid, propionic acid, acetic acid/propionic acid and total volatile acid in the fermentation liquor are changed secondarily (P is less than 0.05). The concentration of isobutyric acid and butyric acid in the fermentation liquor is linearly increased (P < 0.05) along with the increase of the addition level of biotin, and the concentration of isovaleric acid and total branched-chain volatile acid in the fermentation liquor has a tendency of linearly increasing (P < 0.1).
TABLE 3 Effect of daily feed addition of Biotin on rumen fermentation parameters
4.2 Effect of biotin added into daily ration on synthesis of tumor gastric branched chain fatty acid
The effect of different biotin addition levels on the synthesis of tumor gastric branched chain fatty acids is shown in table 4. The fermentation broth was affected by biotin (P < 0.05) for all fatty acids except iso-C14:0 and C23:0 which showed no significant change. The concentrations of ISO-C13:0, ISO-C16:0, ISO-C17:0, anteiso-C15:0, anteiso-C17:0, C15:0, C17:0, and total ISO, total Anteiso, total BCFA, total OCFA, and total OBCFA in the fermentation broth all showed significant linear increase with increasing biotin addition dose (P < 0.05), C21:0 in the fermentation broth showed significant linear increase trend (P < 0.1), and ISO-C13:0, ISO-C16:0, anteiso-C15:0, total Anteiso, and total OCFA concentration showed significant secondary change simultaneously (P < 0.05), ISO-C17:0, anteiso-C17:0, total BCFA, and total OBFA concentration in the fermentation broth showed secondary change (P < 0.1), ISO-C17:0, anteiso-C17:0, total BCFA concentration and OBA concentration showed secondary change (P < 0.1: 0), and the concentration showed secondary change with increasing biotin addition dose in the fermentation broth (P < 0: 1, C13: 1, C < 0: 1).
TABLE 4 Effect of daily feed addition of Biotin on the Synthesis of rumen branched fatty acids
Experimental example 2 experiment of the influence of biotin supplementation in dairy cow ration on the content of odd-chain and branched-chain fatty acids in milk
1. Test animals and designs
In the test, 20 Holstein cows with the same lactation day age, milk yield and gestational age are selected, and the test daily ration is prepared according to NRC (2001) to meet all nutritional requirements (Table 5). All cows were randomly assigned to two treatment groups, 10 cows per treatment group, according to a completely random design. Wherein the control group is fed with basal diet, the test group is added with 80mg biotin every day for each cattle on the basis of the control group, the test pre-feeding period is 14 days, and the test formal period is 28 days. All cows were fed with free food and free water, twice a day. Half of the biotin is mixed with 1kg of daily ration and provided to animals during each feeding, and the rest of the daily ration is provided after the animals completely feed the biotin.
TABLE 5 test Dairy cow diet composition and Nutrition level (Dry matter basis)
2. Sample and data acquisition
2.1 Feed intake and feed samples
The feed addition and the residual feed amount are recorded every day for counting the feed intake. Collecting the total mixed ration and residual material samples every day after the last week of the pre-feeding period and the beginning of the test, and mixing the collected feed samples in equal proportion by taking week as a unit.
2.2 Rumen fluid sample
2.2.1 Sample collection
And collecting rumen fluid samples before the cows eat on the 28 th day of the test, filtering the rumen fluid samples by four layers of gauze, subpackaging the rumen fluid samples into 10mL centrifuge tubes, and storing all the samples at the temperature of-20 ℃ after subpackaging.
2.2.2 Index measurement
The volatile fatty acid was measured in the same manner as in test example 1.
2.3 Milk yield and milk sample
2.3.1 Sample and data acquisition
Milk production was recorded daily at the start of the experiment. Milk samples were collected at milking time on the last day of the pre-feeding period and on day 28 of the experiment and mixed according to the yield per milking, 50mL of which was preserved at 4 ℃ for milk ingredient determination after addition of preservatives, and the remaining samples were dispensed into 10mL centrifuge tubes and stored in-20 ℃ refrigerators.
2.3.2 Milk composition analysis
Milk components in milk were measured using a FOSS milk component analyzer (FT 120).
2.3.3 Milk fatty acid determination
The fatty acid in milk was determined after appropriate modification based on the method of the cow milk fatty acid reference (wang gakai. Ruminant nutrition research methods [ M ]. Modern education press, 2011.).
2.3.4 Reagent
The same as in test example 1.
2.3.5 Sample pretreatment
(1) Accurately weighing 2mL of milk sample (containing 50-100mg fat, accurate to 0.1 mg), adding 4mL of n-hexane-isopropanol mixed solution into a 15mL centrifuge tube with a cover, and carrying out vortex oscillation for 2min.
(2) 2mL of sodium sulfate solution was added, vortexed for 2min, and centrifuged at 5000 Xg for 10min at 4 ℃.
(3) Phase shifting the upper layer of n-hexane to a high temperature resistant test tube with a cover, adding 2mL sodium hydroxide methanol solution, screwing down the test tube cover, shaking up (vortex oscillation for 2 min), and saponifying in 50 deg.C water bath for 30min.
(4) After cooling to room temperature, 2mL of methanol hydrochloride solution was added, the tube cap was tightened and esterified in a water bath at 90 ℃ for 2h.
(5) After cooling, 2mL of water was added, and the mixture was extracted 3 times with 2mL of n-hexane (2 min each time with vortexing), and the n-hexane layers were combined in a 10mL volumetric flask (15 mL inlet centrifuge tube) and the volume was determined with n-hexane.
(6) About 0.5g of anhydrous sodium sulfate was added, vortexed for 30 seconds, allowed to stand (standing for 1 min), and the supernatant was taken as a test solution.
2.3.6 Instrumental determination
The same as in test example 1.
3. Statistical analysis
The experimental data were statistically examined using the MIXED module in the SAS9.4 software. For dry matter feed intake and milk production data, treatment was used as a fixed factor in the statistical model, the number of births of the test cows was a random factor, and the number of days of the test was used as a repeat measure, and the data for each cow during the pretest period was used as a covariate for correction. No duplicate measurements were included in the statistical model of milk composition data. Repeated measurements and covariate corrections were not included in the statistical models of fatty acid and volatile fatty acid data. All statistical results are expressed in the form of least squares means, P <0.05 indicates significant difference, and P <0.10 is more than or equal to 0.05 indicates significant trend.
4. Test results
4.1 Effect of daily ration supplement of biotin on production performance of dairy cows
The effect of biotin addition to dairy cow diets on productivity is shown in table 6. By supplementing biotin in daily ration of the dairy cow, the feed intake and milk yield of the dairy cow and the 4% milk fat correction milk are not significantly influenced (P > 0.05), and the milk components and the yield of related milk components of the test group are not significantly changed (P > 0.05).
TABLE 6 Effect of daily ration Biotin supplementation on Dairy cow production Performance
4.2 Effect of daily ration biotin supplementation on rumen fermentation of dairy cows
According to the test results, the milk cow has no significant effect on rumen fermentation after being supplemented with biotin (table 7).
TABLE 7 influence of daily ration supplementation of cows with biotin on rumen fermentation
4.3 Influence of daily ration biotin supplement on synthesis of odd-chain and branched-chain fatty acids
The odd-chain fatty acid and branched-chain fatty acid content of milk were increased after biotin supplementation in cows (table 8). The test result shows that the content of ISO-C15:0, ISO-C16:0, ISO-C17:0, anteiso-C15:0, anteiso-C17:0, total ISO, total Anteiso, total BCFA and total OBCFA in the milk (P < 0.05) can be obviously improved by adding the biotin into the daily ration of the dairy cow, and the content of ISO-C14:0, C17:0, C23:0 and total OCFA in the milk has a rising trend (P < 0.1) due to the addition of the biotin. The addition of biotin in the daily ration effectively improves the content of total odd-chain fatty acids and branched-chain fatty acids in the milk, wherein the total OCFA, the total BCFA and the total OBCFA are respectively improved by 12.5 percent, 17.9 percent and 16.3 percent.
TABLE 8 Effect of Biotin supplementation in cows on branched-chain fatty acids in milk
The above-described embodiments are merely exemplary and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and substitutions are intended to be within the scope of the invention.
Claims (5)
1. The application of biotin as a feed additive in improving the content of functional fatty acid in milk of dairy cows.
2. The use according to claim 1, wherein the functional fatty acids comprise odd-numbered carbon chains and branched fatty acids.
3. Use according to claim 1, characterized in that it comprises: adding biotin serving as a feed additive into basic ration of the dairy cow, wherein the addition amount of the biotin is 20-80 mg/cow/day.
4. Use according to claim 3, characterized in that it comprises: the addition amount of biotin is 40-80 mg/cow/day.
5. Use according to claim 4, characterized in that it comprises: the addition amount of biotin is 80 mg/cow/day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211635890.4A CN115606691B (en) | 2022-12-19 | 2022-12-19 | Application of biotin as cow feed additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211635890.4A CN115606691B (en) | 2022-12-19 | 2022-12-19 | Application of biotin as cow feed additive |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115606691A true CN115606691A (en) | 2023-01-17 |
| CN115606691B CN115606691B (en) | 2023-07-11 |
Family
ID=84879583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211635890.4A Active CN115606691B (en) | 2022-12-19 | 2022-12-19 | Application of biotin as cow feed additive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115606691B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030007998A1 (en) * | 2001-05-22 | 2003-01-09 | Elliot Block | Unsaturated lipid-enriched feedstock for ruminants |
| US20070123588A1 (en) * | 2003-05-20 | 2007-05-31 | Charles Roe R | Five and fifteen carbon fatty acids for treating metabolic disorders and as nutritional supplements |
| US20140037603A1 (en) * | 2011-04-12 | 2014-02-06 | Doug Bolster | Nutritional compositions including branched chain fatty acids for improving gut barrier function |
| CN110169500A (en) * | 2019-05-09 | 2019-08-27 | 河北冀丰动物营养科技有限责任公司 | A kind of composite premix for cows additive, premix, batch and preparation method |
-
2022
- 2022-12-19 CN CN202211635890.4A patent/CN115606691B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030007998A1 (en) * | 2001-05-22 | 2003-01-09 | Elliot Block | Unsaturated lipid-enriched feedstock for ruminants |
| US20070123588A1 (en) * | 2003-05-20 | 2007-05-31 | Charles Roe R | Five and fifteen carbon fatty acids for treating metabolic disorders and as nutritional supplements |
| US20140037603A1 (en) * | 2011-04-12 | 2014-02-06 | Doug Bolster | Nutritional compositions including branched chain fatty acids for improving gut barrier function |
| CN110169500A (en) * | 2019-05-09 | 2019-08-27 | 河北冀丰动物营养科技有限责任公司 | A kind of composite premix for cows additive, premix, batch and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 罗丽萍等: "生物素在养猪生产中的应用", 猪业科学, vol. 30, no. 3, pages 42 - 43 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115606691B (en) | 2023-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Klein et al. | Docosahexaenoic acid elevates trans-18: 1 isomers but is not directly converted into trans-18: 1 isomers in ruminal batch cultures | |
| Kim et al. | Effect of total mixed ration with fermented feed on ruminal in vitro fermentation, growth performance and blood characteristics of Hanwoo steers | |
| Baert et al. | A study of the structure-activity relationship of oligomeric ellagitannins on ruminal fermentation in vitro | |
| Abo-Donia et al. | Effects of tannins on the fatty acid profiles of rumen fluids and milk from lactating goats fed a total mixed ration containing rapeseed oil | |
| Wattiaux et al. | Fractionation of nitrogen isotopes by mixed ruminal bacteria | |
| CN112042810A (en) | Application of compound feed additive in preparation of feed for improving muscle texture of cultured large yellow croakers | |
| CN108244034A (en) | A kind of goose dietary protein and the feeding method of amino acid dynamic supply | |
| CN115606691A (en) | Application of biotin as cow feed additive | |
| CN101936986B (en) | Method for detecting diazepam and chemoluminescence immunoassay kit special for same | |
| CN114324647B (en) | Simultaneous determination of vitamin K in milk powder 1 And K 2 Methods and uses of (2) | |
| CN102204640A (en) | Novel concentrated feed for raising milk nutritional value and preparation method thereof | |
| Zhang et al. | Effect of different levels of Lactobacillus added to diets on fat deposition and meat quality of Sunit lambs | |
| Lu et al. | Effects of sources and levels of dietary supplementary manganese on growing yak’s in vitro rumen fermentation | |
| He et al. | Lactobacillus rhamnosus GG ATCC53103 and Lactobacillus plantarum JL01 improved nitrogen metabolism in weaned piglets by regulating the intestinal flora structure and portal vein metabolites | |
| Paya et al. | Impact of inulin supplementation on mitigating ruminal emissions of methane | |
| Alimkulo et al. | Development of Resource-Saving Biotechnology for the Production of a Feed Additive from Distiller’s Wastes with Probiotic Properties | |
| CN1308687C (en) | Enzyme linked immuno kit for detecting chloromycetin | |
| CN108967797B (en) | Food capable of calibrating nervonic acid content, high-content nervonic acid solid beverage and preparation method thereof | |
| CN112255328A (en) | Optimized method for measuring fatty acids in different tissues of fish body | |
| CN107788238B (en) | Compound for replacing plasma protein powder, yeast hydrolysate composition, preparation method and application | |
| CN116646023B (en) | Method for evaluating similarity between sample and breast milk in multiple dimensions | |
| Hu et al. | Effects of protein content and the inclusion of protein sources with different amino acid release dynamics on the nitrogen utilization of weaned piglets | |
| Yin et al. | Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models | |
| CN117461733A (en) | Application of 5-hydroxytryptophan in preparing feed additive or feed for promoting growth and improving rumen health of ruminant | |
| CN1690710A (en) | Enzyme linked immuno kit for detecting chloromycetin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |