CN115595256A

CN115595256A - Nucleic acid detecting cassette

Info

Publication number: CN115595256A
Application number: CN202211138912.6A
Authority: CN
Inventors: 吴鹏鹏; 刘海红; 丁松林; 蒋健; 何彦晨
Original assignee: Jiangsu Macro&micro Test Med Tech Co ltd; Hongwei Tex Suzhou Bioengineering Co ltd
Current assignee: Jiangsu Macro&micro Test Med Tech Co ltd; Hongwei Tex Suzhou Bioengineering Co ltd
Priority date: 2022-09-19
Filing date: 2022-09-19
Publication date: 2023-01-13

Abstract

The invention provides a nucleic acid detection card box, which comprises a card box main body, wherein a sample cavity is formed on the card box main body, a reaction structure which is arranged to protrude out of the outer side wall of the card box main body and is provided with a reaction cavity, a plunger cavity for accommodating a plunger and a plurality of accommodating cavities, a valve body accommodating cavity is formed in the bottom end of the plunger cavity, a rotary valve is arranged in the valve body accommodating cavity, a valve body flow channel is formed in the rotary valve, one end of the valve body flow channel is communicated with the plunger cavity, the rotary valve can be controlled to rotate so that the other end of the valve body flow channel can be selectively communicated with one of the sample cavity, the reaction cavity and any one of the accommodating cavities, and the plunger can be controlled to be pushed and pulled in the plunger cavity. The invention realizes the orderly transfer of sample liquid among all chambers by combining the rotary valve with the push-pull operation of the plunger, has simple operation and more compact structural design, thereby being beneficial to the miniaturization design of the card box, being more portable, realizing the instant on-site detection, avoiding the cross infection risk caused by the detection process, and having high detection efficiency and high detection speed.

Description

Nucleic acid detecting cartridge

Technical Field

The invention belongs to the technical field of design of nucleic acid detection instruments, and particularly relates to a nucleic acid detection card box.

Background

The PCR (Polymerase Chain Reaction) technique is a molecular biology technique for amplifying and amplifying specific DNA (deoxyribonucleic acid) sequences in vitro. The PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirement, simplicity, convenience and rapidness, so that the PCR technology is widely applied to molecular biology detection and analysis. Conventional nucleic acid detection needs to be carried out in a PCR laboratory, and according to the requirements of national regulations, the PCR laboratory needs to carry out partition treatment, namely a reagent preparation area, a nucleic acid extraction area, an amplification area and a detection area, and related experimenters need to be provided with PCR on-duty certificates, so that certain requirements are required for the experimental operating environment and the experimental quality of the personnel. However, even if the above strict requirements are observed, it is possible that the accuracy of the detection result is affected because of contamination of the aerosol.

In order to avoid the adverse effect of the pollution of aerosol in the external environment on the detection result, the mode of arranging a plurality of reagent cavities in the nucleic acid detection card box in the prior art realizes the transfer of samples, reagents and the like by using a plurality of on-off valves, and the mode of realizing the transfer by using a plurality of on-off valves is not beneficial to the miniaturization of equipment and the card box.

Disclosure of Invention

Therefore, the invention aims to solve the technical problem that the existing nucleic acid detection cartridge realizes the transfer of samples or reagents by arranging a plurality of on-off valves, so that the cartridge and corresponding equipment are not compact in structure, large in size, not portable enough and the like.

In order to solve the problems, the invention provides a nucleic acid detection card box, which comprises a card box body, wherein a sample cavity, a reaction structure which is arranged to protrude out of the outer side wall of the card box body and is provided with a reaction cavity, a plunger cavity for accommodating a plunger and a plurality of accommodating cavities are formed in the card box body, a valve body accommodating cavity is formed in the bottom end of the plunger cavity, a rotary valve is arranged in the valve body accommodating cavity, a valve body flow channel is formed in the rotary valve, one end of the valve body flow channel is communicated with the plunger cavity, the rotary valve can be controlled to rotate so that the other end of the valve body flow channel can be selectively communicated with one of the sample cavity, the reaction cavity and any one of the accommodating cavities, and the plunger can be controlled to be pushed and pulled in the plunger cavity.

In some embodiments, the cartridge body includes a reagent chamber body and a base assembled at the bottom thereof, the sample chamber, the reaction chamber and the accommodating chamber are all configured on the reagent chamber body, the base is configured with a plurality of flow-through channels respectively in one-to-one communication with the sample chamber, the reaction chamber and the accommodating chamber, and the other end of the valve body flow channel can be selectively communicated with any one of the flow-through channels.

In some embodiments, a first seal is sandwiched between the bottom of the reagent chamber body and the base; and/or a first positioning structure is arranged between the bottom of the reagent cavity main body and the top of the base.

In some embodiments, the top of the rotary valve is provided with a circular arc positioning protrusion, the wall of the valve body accommodating cavity is provided with a circular arc positioning groove matched with the circular arc positioning protrusion, and the circular arc positioning protrusion can be disengaged from the circular arc positioning groove when the rotary valve is controlled to rotate; and/or, the rotary valve has the rotation axis, the rotation axis has and is in the first pivot section at the top of rotary valve, the cover is equipped with first sealing washer in order to realize on the first pivot section with sealed between the bottom in plunger chamber, the rotation axis still has and is in the second pivot section of the bottom of rotary valve, the second pivot section be used for with the drive connection of rotation driving part.

In some embodiments, the base has a first through hole thereon, the overflow channel includes a reaction chamber overflow channel, the first through hole is communicated with the reaction chamber overflow channel, the reaction structure includes a sample feeding pipe, and the sample feeding pipe is detachably inserted into the first through hole.

In some embodiments, a second sealing ring is sleeved between the sample inlet pipe and the first through hole; and/or a plurality of reaction cavities are constructed in the reaction structure, and the reaction cavities are communicated with the sample injection pipes through one-to-many sample injection flow channels respectively.

In some embodiments, the one-to-many sample injection flow channel and the reaction chamber both have an opening facing to one side, and a sealing film is covered and connected on the opening.

In some embodiments, the reagent chamber body is provided with a T-shaped chute on the outside thereof, and the reaction structure further has a connecting flange that can be inserted into the T-shaped chute from the bottom of the reagent chamber body toward the top thereof during assembly of the reaction structure with the cartridge body.

In some embodiments, the reagent chamber main body is further configured with an air hole, the cartridge main body further comprises an upper cover assembled with the reagent chamber main body, the upper cover is provided with a sample adding hole correspondingly communicated with the top opening of the sample chamber and a plunger rod through hole correspondingly communicated with the top opening of the plunger chamber, the sample adding hole is provided with a sealing cover capable of being opened and closed, the sealing cover is provided with an air passing hole, and gas discharged from the reaction chamber can be discharged from the air passing hole through the air hole.

In some embodiments, the accommodating chamber and the air hole both have top openings, the top openings are covered and connected with aluminum foil sealing paper, the upper cover is provided with piercing heads which are respectively arranged corresponding to the air hole and the accommodating chamber one by one, when the upper cover is assembled with the reagent chamber main body, the piercing heads can pierce the aluminum foil sealing paper, and each piercing head is provided with a gas passing channel communicated with the corresponding accommodating chamber or air hole; and/or the reaction structure is also provided with an exhaust pipe, and a PE sintering plug is arranged in the exhaust pipe and inserted into the air hole.

According to the nucleic acid detection card box provided by the invention, the communication of one of the containing cavities, the sample cavity and the reaction cavity with the plunger cavity is realized through the rotary valve capable of being controlled to rotate, the ordered transfer of sample liquid among the cavities is realized by combining the push-pull operation of the plunger, the operation is simple, the structural design is more compact, the miniaturization design of the card box is facilitated, and the card box is more portable.

Drawings

FIG. 1 is a schematic view showing the structure of a nucleic acid detecting cassette according to an embodiment of the present invention;

FIG. 2 is an exploded view of FIG. 1;

FIG. 3 is a schematic perspective view of the reagent chamber body of FIG. 2;

FIG. 4 is a top view of FIG. 3;

FIG. 5 isbase:Sub>A cross-sectional view A-A of FIG. 4;

FIG. 6 is a cross-sectional view of the rotary valve of FIG. 2;

FIG. 7 is a perspective view of the base of FIG. 2;

FIG. 8 is a schematic perspective view of the upper cover of FIG. 2;

FIG. 9 is a bottom view of the upper cover of FIG. 8;

FIG. 10 is a schematic perspective view of the reaction structure of FIG. 2;

FIG. 11 is another schematic perspective view of the reaction structure of FIG. 2.

The reference numerals are represented as:

1. a cartridge body; 11. a sample chamber; 12. a plunger cavity; 121. a valve body receiving cavity; 13. air holes; 141. a common cavity; 142. a first washing chamber; 143. a second washing chamber; 144. an elution chamber; 145. a freeze-drying ball cavity; 2. a plunger; 3. rotating the valve; 31. a valve body flow passage; 321. a circular arc positioning bulge; 331. a first shaft segment; 332. a first seal ring; 333. a second shaft section; 4. a reaction structure; 41. a reaction chamber; 42. a sample inlet pipe; 43. a one-to-many sample introduction flow passage; 44. sealing the film; 45. a connecting flange; 46. an exhaust pipe; 51. a reagent chamber body; 511. a T-shaped chute; 52. a base; 521. a first through hole; 522. a reaction chamber overflow channel; 523. a sample chamber flow passage; 524. a common chamber flow passage; 525. a first washing chamber overflow channel; 526. a second washing chamber flow passage; 527. an eluent flow passage; 528. a freeze-drying ball cavity flow passage; 529. a positioning column; 53. a second seal ring; 54. an upper cover; 541. a sample application hole; 542. a plunger rod through hole; 543. a sealing cover; 5431. air passing holes; 544. piercing the head; 545. a converging duct; 61. a first gasket; 62. a second gasket.

Detailed Description

Referring to fig. 1 to 11 in combination, according to an embodiment of the present invention, there is provided a nucleic acid detecting cartridge, which includes a cartridge body 1, the cartridge body 1 is configured with a sample chamber 11, a reaction structure 4 protruding from an outer side wall of the cartridge body 1 and having a reaction chamber 41, a plunger chamber 12 for accommodating a plunger 2 (preferably a rubber plunger with good sealing), and a plurality of accommodating chambers, a bottom end of the plunger chamber 12 has a valve accommodating chamber 121, a rotary valve 3 is disposed in the valve accommodating chamber 121, a valve flow channel 31 is configured in the rotary valve 3, one end of the valve flow channel 31 is communicated with the plunger chamber 12, the rotary valve 3 can be controlled to rotate so that the other end of the valve flow channel 31 can be selectively communicated with one of the sample chamber 11, the reaction chamber 41, and any one of the accommodating chambers, and the plunger 2 can be controlled to be pushed and pulled in the plunger chamber 12, and the aforementioned accommodating chambers include, for example, a first washing chamber 142, a second washing chamber 143, an elution chamber 144, and a freeze-drying ball chamber 145, and a common chamber 141. Among this technical scheme, realize each holding chamber through rotary valve 3 that can be controlled the rotation, sample chamber 11, one in the reaction chamber 41 and the intercommunication in plunger chamber 12, the push-and-pull operation that combines plunger 2 has realized the orderly transfer of sample liquid between each cavity, moreover, the steam generator is simple in operation, structural design is compacter, thereby do benefit to the miniaturized design of card box, and is more portable, because this move the liquid process and go on in card box main part 1, thereby the requirement to the experimental environment has been reduced, effectively prevent aerosol pollution, can realize instant on-the-spot detection, can avoid the testing process and cause the cross infection risk, detection efficiency is high, detection speed is fast.

Referring to fig. 2, the cartridge body 1 includes a reagent chamber body 51 and a base 52 assembled at the bottom thereof, the sample chamber 11, the reaction chamber 41 and the accommodating chamber are all constructed on the reagent chamber body 51, the base 52 is constructed with a plurality of flow channels communicated with the sample chamber 11, the reaction chamber 41 and the accommodating chamber in a one-to-one correspondence manner, the other end of the valve body flow channel 31 can be selectively communicated with any one of the plurality of flow channels, and the reagent chamber body 51 and the base 52 can be assembled quickly by means of a snap-fit manner. In this technical solution, a plurality of overflow channels are formed on one side of the base 52 facing the reagent chamber main body 51, so as to reduce the processing difficulty of the overflow channels, and it can be understood that the positions of the overflow channels should be adapted to the other ends of the chambers and the valve body flow channel 31, specifically, the first ends of the overflow channels should be set and communicated with the sample chamber 11, the common chamber 141, the first washing chamber 142, the second washing chamber 143, the elution chamber 144, and the freeze-drying ball chamber 145, and the second ends of the overflow channels should be set along the position of the circle center track formed by the rotation of the other ends of the valve body flow channel 31, so as to realize the controllable communication between the chambers and the plunger chamber 12 by the rotation of the rotary valve 3, and further ensure that the pipetting is performed according to the sequence of nucleic acid extraction. Referring to fig. 7, the aforementioned flow-through channels may specifically include a sample chamber flow-through channel 523, a common chamber flow-through channel 524, a first wash chamber flow-through channel 525, a second wash chamber flow-through channel 526, an eluent flow-through channel 527, a freeze-dried ball chamber flow-through channel 528, and a reaction chamber flow-through channel 522.

In some embodiments, a first sealing pad 61 is clamped between the bottom of the reagent chamber main body 51 and the base 52 to ensure the connection sealing performance between the reagent chamber main body 51 and the base 52, it can be understood that, the first sealing pad 61 is configured with flow through holes (not shown in the figures) which are simultaneously communicated with the flow through channels and the corresponding chambers, the bottom side surface of the rotary valve 3 is attached to the top surface of the first sealing pad 61, and the first sealing pad 61 has certain elasticity, and a silicone pad can be specifically used.

A first positioning structure is arranged between the bottom of the reagent chamber main body 51 and the top of the base 52, and specifically, as shown in fig. 7, two positioning posts 529 are respectively arranged at two diagonal positions of the base 52, and correspondingly, corresponding positioning holes are formed at the bottom of the reagent chamber main body 51, and when the reagent chamber main body 51 and the base 52 are assembled, the positioning posts 529 can be inserted into the positioning holes, so as to form the assembling and positioning of the two. In another specific embodiment, the base 52 and the reagent chamber body 51 are snap-fit connected to each other for quick assembly.

Referring to fig. 6, the top of the rotary valve 3 has an arc positioning protrusion 321, and the cavity wall of the valve body accommodating cavity 121 has an arc positioning groove (not shown, not indexed) adapted to the arc positioning protrusion 321, so that when the rotary valve 3 is controlled to rotate, the arc positioning protrusion 321 can be disengaged from the arc positioning groove, it can be understood that, during the assembly process, the rotary valve 3 is inserted into the corresponding arc positioning groove via the arc positioning protrusion 321, so as to ensure the initial rotation angle of the rotary valve 3, and further enable the subsequent cavity communication selection to be more accurate, and the positioning structure can easily release the positioning relationship due to the elastic deformation of the first sealing gasket 61.

With further reference to fig. 2 and fig. 6, the rotary valve 3 has a rotary shaft, the rotary shaft has a first rotary shaft section 331 at the top of the rotary valve 3, the first rotary shaft section 331 is sleeved with a first sealing ring 332 to seal between the first rotary shaft section 331 and the bottom of the plunger cavity 12, the rotary shaft further has a second rotary shaft section 333 at the bottom of the rotary valve 3, the second rotary shaft section 333 is used for driving connection with a rotary driving component, in a specific application process, the corresponding rotary driving component may employ a stepping motor, the stepping motor is disposed on a corresponding instrument, and the stepping motor forms driving connection with the second rotary shaft section 333 only in a specific detection process (for example, the rotary shaft of the motor is connected with the second rotary shaft section 333 through a coupling sleeve and other coupling structures). The first sealing ring 332 is sleeved on the first rotating shaft section 331, so that leakage of the solution sucked in the plunger cavity 12 can be effectively prevented, and the quantitative accuracy in the pipetting process is ensured. The first seal ring 332 may be an O-ring.

Referring to fig. 7 and 10, the base 52 has a first through hole 521, the first through hole 521 is communicated with the reaction chamber overflow channel 522, the reaction structure 4 includes a sample injection tube 42, and the sample injection tube 42 is detachably inserted into the first through hole 521. Specifically, through the detachable connection of the sample injection tube 42 and the base 52, different reaction structures 4 can be replaced according to actual detection items, and the cartridge body 1 is the same, so that the universality of the cartridge body 1 is effectively improved, and the use cost is reduced.

In some embodiments, a second sealing ring 53 is sleeved between the sample inlet tube 42 and the first through hole 521 to prevent the sample solution from leaking out at the position where the sample inlet tube and the first through hole are connected.

The reaction structure 4 may be formed by injection molding, and is a flat structure as a whole to cooperate with an external temperature control module to ensure accurate temperature control, in a specific embodiment, as shown in fig. 10, only one reaction chamber 41 is configured in the reaction structure 4, and an inner space of the reaction chamber 41 is communicated with the sample injection pipe 42; in another specific embodiment, referring to fig. 11, a plurality of reaction chambers 41 are configured in the reaction structure 4, and the reaction chambers 41 and the sample injection tubes 42 are respectively in parallel communication through one-to-many sample injection flow channels 43, that is, a multi-channel reaction chamber structure is realized, so that multi-channel detection can be realized, and it can be understood that the reaction chambers 41 further have corresponding exhaust structures to ensure smooth liquid injection. Specifically, the one-to-many sample injection channel 43 is, for example, one-to-two, two-to-four, and the number of the corresponding reaction chambers 41 is multiplied by 2.

Referring to fig. 2, the one-to-many sample inlet channel 43 and the reaction chamber 41 each have an opening facing one side, and a sealing film 44 is covered and connected on the opening, specifically, the sealing film 44 is connected to the opening side of the reaction structure 4 by ultrasonic welding or thermal bonding, so that the difficulty in manufacturing the reaction structure 4 can be reduced.

Referring to fig. 1 and 10, the reagent chamber body 51 is provided with a T-shaped sliding slot 511 at the outer side thereof, and the reaction structure 4 further has a connecting flange 45, wherein the connecting flange 45 can be inserted into the T-shaped sliding slot 511 from the bottom of the reagent chamber body 51 toward the top thereof in the process of assembling the reaction structure 4 and the cartridge body 1. In the technical solution, during the specific assembly, the sample inlet tube 42 of the reaction structure 4 is inserted into the first through hole 521 of the base 52, and then the reagent chamber main body 51 is fastened with the base 52 from top to bottom, and in the process, the T-shaped sliding slot 511 is synchronously sleeved on the outer side of the connecting flange 45, so that the forming position of the connecting flange 45 can be limited, and the assembly reliability between the reaction structure 4 and the cartridge main body 1 is ensured.

Referring to fig. 8 and 9, the reagent chamber body 51 is further configured with an air hole 13, the cartridge body 1 further includes an upper cover 54 assembled with the reagent chamber body 51, the upper cover 54 is provided with a sample adding hole 541 correspondingly communicated with the top opening of the sample chamber 11 and a plunger rod through hole 542 correspondingly communicated with the top opening of the plunger chamber 12, the sample adding hole 541 is provided with a sealing cover 543 capable of opening and closing, the sealing cover 543 is provided with an air passing hole 5431 (which can pass through a gas film by ultrasonic welding and allow gas flow but not liquid flow), gas exhausted from the reaction chamber 41 can be exhausted from the air passing hole 5431 through the air hole 13, so that gas evacuation in the reaction chamber 41 during sample introduction is ensured, and smooth sample introduction is ensured.

In some embodiments, the accommodating chambers and the air holes 13 both have top openings, the top openings are covered and connected with aluminum foil sealing paper, so as to ensure a sealed environment before the upper cover 54 is assembled with the reagent chamber main body 51, the upper cover 54 has piercing heads 544 corresponding to the air holes 13 and the accommodating chambers one by one, when the upper cover 54 is assembled with the reagent chamber main body 51, the piercing heads 544 can pierce the aluminum foil sealing paper, each piercing head 544 has a gas passage communicated with the corresponding accommodating chamber or air hole 13, so that ventilation of each accommodating chamber in the use process of the cartridge is realized, and smooth liquid transfer is ensured. It should be noted that the above-mentioned aluminum foil sealing paper and the upper cover 54 are designed to make each accommodating chamber of the cartridge in a closed state before, during and after use, so as to effectively prevent the occurrence of aerosol contamination.

Referring to fig. 10, the reaction structure 4 further has an exhaust pipe 46, and a PE sintered plug (allowing gas to pass but not liquid to pass) is disposed in the exhaust pipe 46 and inserted into the gas hole 13, so that the gas in the reaction chamber 41 is exhausted through the gas hole 13. Further, each piercing head 544 has a gas passage gathered at the sample application hole 541, and specifically, the gas passage is gathered and communicated with the sample application hole 541 through a confluence pipeline 545, the confluence pipeline 545 has an opening facing to the reagent chamber main body 51, and the opening is covered and hermetically connected (bonded or ultrasonically welded) with a second sealing pad 62, so as to optimize the structure of the upper cover 54 and simplify the formation process of each passage.

In order to reduce the manufacturing cost of the detection cartridge, the reagent chamber body 51, the plunger 2, the rotary valve 3, the reaction structure 4, the base 52, the upper cover 54, and the like in the cartridge body 1 are all manufactured by injection molding, so that they can be discarded as medical waste after use.

The technical solution of the present invention is further explained in connection with the use process below.

The operator needs to assemble the reaction structure 4, the base 52 and the reagent chamber main body 51 to form a first assembly component according to the requirement of nucleic acid detection, and then combine the first assembly component, the upper cover 54 and the second sealing gasket 62 (the two components form a second assembly component) to form a whole body to form the nucleic acid detection cassette of the present invention; the sample is added into the sample cavity 11 from the sample adding hole 541 of the upper cover 54, the sealing cover 543 of the upper cover 54 is covered after the sample adding is finished, the card box after the sample adding is put into the corresponding instrument, and the instrument automatically finishes the processes of nucleic acid extraction and amplification and result display.

The specific working principle is as follows:

the device is provided with a plunger rod, the plunger rod pushes the plunger 2 to enable the chambers of the containing cavities to generate pressure difference with the plunger cavity 12, the rotary valve 3 is driven to rotate by the rotation of a device motor (the stepping motor), one end of a liquid path of the rotary valve 3 (namely the valve body flow channel 31) is communicated with the flow channel openings distributed on the base 52 according to the circumference through the rotation angle of the motor, and the other end of the liquid path is communicated with the plunger cavity 12, so that the liquid is transferred from one cavity to the other cavity. When the device is used, the rotating motor of the device firstly leads one end of the rotary valve 3 to be communicated with the flow passage opening of the sample cavity 11, pushes the plunger to generate pressure difference, leads the liquid in the sample cavity 11 to enter the plunger cavity 12, and the motor rotates to drive the rotary valve to rotate to a specific angle, and then the reagent in the plunger cavity 12 is transferred into the common cavity 141 by pushing the plunger rod. The magnetic beads (not shown in the figure) are arranged in the public cavity 141 in advance, after the nucleic acid is combined with the magnetic beads, the magnetic bead adsorption device of the instrument adsorbs the magnetic beads, the motor rotates to drive the rotary valve 3 to rotate to a specific angle, the plunger is pushed to generate pressure difference, liquid is sucked into the plunger cavity 12, the motor rotates to drive the rotary valve 3 to rotate to a specific angle, the plunger is pushed to generate pressure difference, and then the liquid is transferred to the sample cavity 11.

The motor rotation drives rotary valve 3 and rotates to specific angle, promote the plunger and produce the pressure differential, change over to first washing chamber 142 in liquid to plunger chamber 12, the motor rotation drives rotary valve 3 and rotates to specific angle, it produces the pressure differential to promote the plunger, shift liquid to common chamber 141 in, the magnetic bead adsorption equipment of instrument adsorbs the magnetic bead after the washing is accomplished, the motor rotation drives rotary valve 3 and rotates to specific angle, it produces the pressure differential to promote the plunger, inhale plunger chamber 12 to liquid, the motor rotation drives rotary valve 3 and rotates to specific angle, it produces the pressure differential to promote the plunger, and then shift liquid to first washing chamber 142 in.

The motor rotation drives rotary valve 3 and rotates to specific angle, promote the plunger and produce the pressure differential, liquid changes over to plunger chamber 12 in the second washing chamber 143, the motor rotation drives rotary valve 3 and rotates to specific angle, it produces the pressure differential to promote the plunger, shift liquid to common chamber 141 in, the magnetic bead adsorption equipment of instrument adsorbs the magnetic bead after the washing is accomplished, the motor rotation drives rotary valve 3 and rotates to specific angle, it produces the pressure differential to promote the plunger, inhale plunger chamber 12 to liquid, the motor rotation drives rotary valve 3 and rotates to specific angle, it produces the pressure differential to promote the plunger, and then shift liquid to in the second washing chamber 143.

The motor rotates to drive the rotary valve 3 to rotate to a specific angle, the plunger is pushed to generate pressure difference, liquid in the elution cavity 144 is transferred to the plunger cavity 12, the motor rotates to drive the rotary valve 3 to rotate to the specific angle, the plunger is pushed to generate the pressure difference, the liquid is transferred to the common cavity 141, a magnetic bead adsorption device of the instrument adsorbs magnetic beads after washing is completed, the motor rotates to drive the rotary valve 3 to rotate to the specific angle, the plunger is pushed to generate the pressure difference, the liquid is sucked into the plunger cavity 12, the motor rotates to drive the rotary valve 3 to rotate to the specific angle, the plunger is pushed to generate the pressure difference, and the liquid is transferred to the freeze-drying ball cavity 145 to complete freeze-drying ball redissolution.

The motor rotates to drive the rotary valve 3 to rotate to a specific angle, the plunger is pushed to generate pressure difference, liquid in the elution cavity 144 is transferred to the plunger cavity 12, the motor rotates to drive the rotary valve 3 to rotate to a specific angle, the plunger is pushed to generate pressure difference, and the liquid is transferred to the reaction cavity 41. After that, the subsequent detection processes such as amplification reaction and the like may be further performed on the liquid in the reaction chamber 41 through a temperature control module provided in the apparatus, and these processes are well known in the art and are not described in detail.

It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, it is possible to make several improvements and modifications without departing from the technical principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.

Claims

1. The nucleic acid detection card box is characterized by comprising a card box body (1), wherein a sample cavity (11), a reaction structure (4) which protrudes out of the outer side wall of the card box body (1) and is provided with a reaction cavity (41), a plunger cavity (12) for accommodating a plunger (2) and a plurality of accommodating cavities are constructed on the card box body (1), a valve body accommodating cavity (121) is formed in the bottom end of the plunger cavity (12), a rotary valve (3) is arranged in the valve body accommodating cavity (121), a valve body flow channel (31) is constructed in the rotary valve (3), one end of the valve body flow channel (31) is communicated with the plunger cavity (12), the rotary valve (3) can be controlled to rotate so that the other end of the valve body flow channel (31) can be selectively communicated with one of the sample cavity (11), the reaction cavity (41) and any one of the accommodating cavities, and the plunger (2) can be controlled to be pushed and pulled in the plunger cavity (12).

2. The nucleic acid detecting cartridge according to claim 1, wherein the cartridge body (1) includes a reagent chamber body (51) and a base (52) assembled at the bottom thereof, the sample chamber (11), the reaction chamber (41) and the accommodating chamber are all configured on the reagent chamber body (51), the base (52) is configured with a plurality of flow-through channels respectively in one-to-one communication with the sample chamber (11), the reaction chamber (41) and the accommodating chamber, and the other end of the valve body flow channel (31) can be selectively in communication with any one of the plurality of flow-through channels.

3. The nucleic acid detecting cartridge according to claim 2, wherein a first seal gasket (61) is sandwiched between the bottom of the reagent chamber main body (51) and the base (52); and/or a first positioning structure is arranged between the bottom of the reagent cavity main body (51) and the top of the base (52).

4. The nucleic acid detecting cartridge according to claim 3, wherein the rotary valve (3) has a circular arc positioning protrusion (321) at the top, and the wall of the valve body accommodating chamber (121) has a circular arc positioning groove fitted with the circular arc positioning protrusion (321), and the circular arc positioning protrusion (321) can be disengaged from the circular arc positioning groove when the rotary valve (3) is controlled to rotate; and/or, the rotary valve (3) has a rotary shaft, the rotary shaft has a first rotary shaft section (331) at the top of the rotary valve (3), the first rotary shaft section (331) is sleeved with a first sealing ring (332) to realize the sealing between the first rotary shaft section (331) and the bottom of the plunger cavity (12), the rotary shaft also has a second rotary shaft section (333) at the bottom of the rotary valve (3), and the second rotary shaft section (333) is used for being connected with the drive of the rotary drive component.

5. The nucleic acid detecting cartridge according to claim 2, wherein the base (52) has a first through-hole (521) therein, the flow-passing channel includes a reaction chamber flow-passing channel (522), the first through-hole (521) communicates with the reaction chamber flow-passing channel (522), the reaction structure (4) includes a sample introduction tube (42), and the sample introduction tube (42) is detachably inserted into the first through-hole (521).

6. The nucleic acid detecting cartridge according to claim 5, wherein a second seal ring (53) is fitted between the sampling tube (42) and the first through hole (521); and/or a plurality of reaction cavities (41) are constructed in the reaction structure (4), and the reaction cavities (41) and the sample feeding pipes (42) are respectively communicated in parallel through one-to-many sample feeding flow passages (43).

7. The nucleic acid detecting cartridge according to claim 6, wherein the divided-into sample flow path (43) and the reaction chamber (41) each have an opening facing one side, and a sealing film (44) is attached to the opening in a covering manner.

8. The nucleic acid detecting cartridge according to claim 5, wherein the reagent chamber main body (51) is provided at an outer side thereof with a T-shaped slide groove (511), and the reaction structure (4) further has a connecting flange (45), and the connecting flange (45) is insertable into the T-shaped slide groove (511) from a bottom of the reagent chamber main body (51) toward a top thereof during assembly of the reaction structure (4) with the cartridge main body (1).

9. The nucleic acid detecting cartridge according to any one of claims 2 to 8, wherein the reagent chamber main body (51) is further provided with an air vent (13), the cartridge main body (1) further comprises an upper cover (54) assembled with the reagent chamber main body (51), the upper cover (54) is provided with a sample adding hole (541) correspondingly communicated with the top opening of the sample chamber (11) and a plunger rod through hole (542) correspondingly communicated with the top opening of the plunger chamber (12), the sample adding hole (541) is provided with an openable and closable sealing cover (543), the sealing cover (543) is provided with an air vent (5431), and air exhausted from the reaction chamber (41) can be exhausted from the air vent (5431) through the air vent (13).

10. The cartridge of claim 9, wherein the receiving chamber and the air hole (13) each have a top opening covered with an aluminum foil sealing paper, the upper cover (54) has a piercing head (544) disposed corresponding to the air hole (13) and the receiving chamber, respectively, the piercing head (544) can pierce the aluminum foil sealing paper when the upper cover (54) is assembled with the reagent chamber body (51), and each piercing head (544) has an air passage communicating with the corresponding receiving chamber or air hole (13); and/or an exhaust pipe (46) is further arranged on the reaction structure (4), and a PE sintered plug is arranged in the exhaust pipe (46) and is inserted into the air hole (13).