CN112934285A - Full-automatic totally-enclosed fluorescence quantitative PCR microfluidic diagnosis chip - Google Patents
Full-automatic totally-enclosed fluorescence quantitative PCR microfluidic diagnosis chip Download PDFInfo
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- CN112934285A CN112934285A CN202110383276.2A CN202110383276A CN112934285A CN 112934285 A CN112934285 A CN 112934285A CN 202110383276 A CN202110383276 A CN 202110383276A CN 112934285 A CN112934285 A CN 112934285A
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a full-automatic totally-enclosed fluorescent quantitative PCR (polymerase chain reaction) microfluidic diagnosis chip, which comprises a microchannel component, wherein the top and the lower part of the microchannel component are provided with a plurality of cabin bodies, the surface of the microchannel component is provided with a plurality of microchannels, and each microchannel is communicated with one cabin body; the surface of the micro-channel component is provided with a first channel and a second channel, and the first channel is communicated with a bin body provided with an injection piston at the top; the micro-channel component is provided with a switching valve mounting position, and a channel switching valve and a reagent switching valve which can rotate relative to the micro-channel component are arranged on the micro-channel component; a plurality of dry reagent bins are distributed on the reagent switching valve along the circumference, and the dry reagent bins can be communicated with the first flow channel and the second flow channel through rotation; an annular common flow channel is arranged on the surface of the flow channel switching valve and is communicated with the second flow channel, the annular common flow channel is provided with a flow channel selection port extending along the radial direction of the flow channel switching valve, and the flow channel rotation port can be communicated with any micro flow channel through rotation. The chip integrates nucleic acid extraction, amplification and detection, reduces pollution, and simultaneously reduces the difficulty and cost of nucleic acid detection.
Description
Technical Field
The invention belongs to the field of in-vitro diagnosis, and particularly relates to a full-automatic fully-closed fluorescent quantitative PCR microfluidic diagnosis chip.
Background
The conventional flow of nucleic acid detection is as follows: sampling a sample, storing and processing the sample, extracting nucleic acid, configuring reagents, amplifying the nucleic acid, analyzing an amplification product, processing the amplification product and the like. The factors affecting the result of the PCR experiment are many, and one of the very important factors is the pollution of nucleic acid aerosol during the PCR experiment. Since trace contamination of nucleic acid aerosol may cause false positive of the detection result and even failure of the detection experiment, each stage of nucleic acid detection usually needs to be completed in a specially configured PCR laboratory. The laboratory needs to realize the partition operation of sample treatment, nucleic acid extraction and PCR amplification, and has a good ventilation system, the cost for building the laboratory is very high, and the financial resources are often built only by large-scale medical institutions.
Microfluidic technology refers to a technology for manipulating a trace amount of fluid using micro flow channels and various microstructures, and is an emerging interdiscipline related to chemistry, fluid physics, microelectronics, new materials, biology and biomedical engineering. Because of the characteristics of miniaturization, integration and the like, the microfluidic device is generally called a microfluidic chip or a lab-on-a-chip, and a small microfluidic chip is equivalent to the whole process of automatically completing analysis by integrating basic operations such as sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes together in a concentrating manner in cooperation with an instrument. The microfluidic chip generally has the advantages of less sample consumption, high detection speed, simple and convenient operation, multifunctional integration, small volume, convenience in carrying and the like, so that the microfluidic chip has great potential of simplifying a diagnosis process, reducing medical cost and improving medical efficiency.
If the microfluidic technology can be used in the nucleic acid detection process, the requirements on a laboratory are greatly reduced, the difficulty and the cost of nucleic acid detection can be reduced, the popularization of nucleic acid detection to primary hospitals is facilitated, and the method makes a great contribution to the detection of various infectious diseases.
Disclosure of Invention
The technical problem solved by the invention is as follows: the totally enclosed fluorescent quantitative PCR microfluidic diagnosis chip integrates nucleic acid extraction, amplification and detection, reduces pollution, and simultaneously reduces difficulty and cost of nucleic acid detection.
The technical scheme adopted by the invention is as follows:
a full-automatic totally-enclosed fluorescence quantitative PCR microfluidic diagnosis chip comprises a microchannel component, wherein a plurality of cabin bodies are arranged at the top and the lower part of the microchannel component, a plurality of microchannels are arranged on the surface of the microchannel component, and each microchannel is communicated with one cabin body; the middle part of the surface of the micro-channel component is also provided with a first channel and a second channel, the first channel is communicated with one bin body at the top, and an injection piston is arranged in the bin body;
the micro-channel assembly is also provided with a switching valve mounting position, and the switching valve mounting position is provided with a channel switching valve and a reagent switching valve which can rotate relative to the micro-channel assembly; a plurality of dry reagent bins are distributed on the reagent switching valve along the circumference, and the dry reagent bins can be communicated with the first flow channel and the second flow channel through rotation; the surface of the flow channel switching valve is provided with an annular common flow channel which is communicated with the second flow channel, the annular common flow channel is provided with a flow channel selection port which extends along the radial direction of the flow channel switching valve, and the flow channel rotation port can be communicated with any micro flow channel through rotation.
Preferably, the bin body at the top of the runner assembly comprises a liquid transfer bin, a plurality of reagent bins and a sample bin, and an injection piston is arranged in the liquid transfer bin; the storehouse body of runner subassembly lower part includes PCR amplification detection storehouse, and reaction storehouse, the reaction storehouse is including nucleic acid extraction storehouse and the multi-functional storehouse of intercommunication each other.
Preferably, the upper part of the surface of the micro-channel component is also provided with a gas internal circulation hole, the gas internal circulation hole is communicated with a gas micro-channel, and the gas micro-channel is communicated with the multifunctional bin.
Preferably, the surface of the flow channel switching valve is also provided with a ventilation flow channel, the micro flow channel connected with the gas circulation inner hole is of a three-way type, the ventilation flow channel can be communicated with the free end of the three-way type micro flow channel by rotating the flow channel switching valve, and the ventilation flow channel is communicated with the PCR amplification detection bin through the three-way type micro flow channel.
Preferably, the micro-channel assembly further comprises a reagent sealing film, and the reagent sealing film is arranged at the tops of the plurality of reagent bins.
Preferably, the microchannel component comprises a chip main body and a microchannel sealing layer which are assembled into a whole, wherein the chip main body is provided with a plurality of cabin bodies, microchannels, a first channel and a second channel; the runner sealing layer is provided with a reagent bin inlet and a reagent bin outlet, the reagent bin inlet is communicated with the first runner, the reagent bin outlet is communicated with the second runner, and the reagent switching valve enables the dry reagent bin to be communicated with the reagent bin inlet and the reagent bin outlet through rotation.
Preferably, the chip main body and the flow channel sealing layer are combined together in any one of ultrasonic welding, bonding or hot melting to form a complete flow channel to form the micro-flow channel assembly.
Preferably, the PCR microfluidic diagnostic chip further comprises a chip upper shell assembly arranged on the upper part of the microchannel assembly, the chip upper shell assembly comprises a chip upper shell, a chip sealing cover is arranged on the chip upper shell, and the chip sealing cover corresponds to the sample bin; and a sealing ring is arranged between the chip upper shell component and the micro-channel component.
Preferably, the chip upper shell assembly further comprises a sealing elastic film and a sealing film pressing plate, the sealing film pressing plate is provided with a plurality of through holes, the through holes correspond to the reagent bins, and the sealing elastic film is further arranged on the sealing film pressing plate.
Preferably, the PCR microfluidic diagnostic chip further comprises a chip lower shell matched with the microchannel assembly.
The beneficial technical effects of the invention are as follows:
by using the microfluidic technology on the nucleic acid detection chip, the chip reduces the expensive and expensive PCR laboratory required by the traditional PCR experiment to a small test chip, realizes the automation of detection, greatly reduces the requirements on the laboratory, also reduces the difficulty and the cost of nucleic acid detection, is favorable for the popularization of nucleic acid detection to primary hospitals, and particularly makes great contribution to the detection of various infectious diseases.
Drawings
FIG. 1 is a schematic diagram of a chip;
FIG. 2 is a schematic view of a chip assembly;
FIG. 3 is a schematic diagram of a chip upper shell assembly;
FIG. 4 is a schematic view of a micro flow channel assembly of the chip;
fig. 5 is a schematic view of a chip main body structure.
Wherein, the names corresponding to the reference numbers are:
1-chip upper shell component, 2-micro flow channel component, 3-chip lower shell three, 1-1-chip sealing cover, 1-2-chip upper shell, 1-3-sealing elastic film, 1-4-sealing film pressing plate, 2-1-chip main body, 2-2-sealing ring, 2-3-reagent sealing film, 2-4-injection piston, 2-5-flow channel switching valve, 2-6-flow channel sealing layer, 2-7-reagent switching valve, 2-8-freeze-drying reagent, 2-1-1-liquid transfer bin, 2-1-2-reagent bin, 2-1-3-sample bin, 2-1-4-gas internal circulation hole, 2-1-5-micro flow channel, 2-1-6-switching valve installation position, 2-1-7-PCR amplification detection bin, 2-1-8-nucleic acid extraction bin, 2-1-9-multifunctional bin, 2-1-10-flow channel I, 2-1-11-flow channel II, 2-5-1 annular common flow channel, 2-5-2 flow channel selection port, 2-5-3 ventilation flow channel, 2-6-1 reagent bin inlet and 2-6-2 reagent bin outlet.
Detailed Description
The present invention will be further described with reference to the following description and examples, which include but are not limited to the following examples.
The invention adopts the microfluidic technology to concentrate and integrate the basic operations of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis, and the whole analysis process is automatically completed by matching with an instrument, the specific structure of the invention is shown in figures 1-5,
a full-automatic totally-enclosed fluorescent quantitative PCR microfluidic diagnosis chip comprises a microchannel component 2, wherein a plurality of cabin bodies are arranged at the top and the lower part of the microchannel component 2, a plurality of microchannels 2-1-5 are arranged on the surface of the microchannel component 2, and each microchannel 2-1-5 is communicated with one cabin body; the middle part of the surface of the micro-channel component 2 is also provided with a first flow channel 2-1-10 and a second flow channel 2-1-11, the first flow channel 2-1-10 is communicated with one bin body at the top, and an injection piston 2-4 is arranged in the bin body;
the micro flow channel assembly 2 is also provided with a switching valve installation position 2-1-6, and the switching valve installation position 2-1-6 is provided with a flow channel switching valve 2-5 and a reagent switching valve 2-7 which can rotate relative to the micro flow channel assembly 2; a plurality of dry reagent bins are circumferentially distributed on the reagent switching valve 2-7, and the dry reagent bins can be communicated with a first flow channel 2-1-10 and a second flow channel 2-1-11 through rotation; the surface of the flow channel switching valve 2-5 is provided with an annular common flow channel 2-5-1, the annular common flow channel 2-5-1 is communicated with a flow channel II 2-1-11, the annular common flow channel 2-5-1 is provided with a flow channel selection port 2-5-2 extending along the radial direction of the flow channel switching valve, and the flow channel rotation port 2-5-2 can be communicated with a micro flow channel 2-1-5 through rotation.
Further, when nucleic acid detection is carried out, the chamber body at the top of the flow channel assembly is divided into a liquid transfer chamber 2-1-1, a plurality of reagent chambers 2-1-2 and a sample chamber 2-1-3, and an injection piston 2-4 is arranged in the liquid transfer chamber 2-1-1; the chamber body at the lower part of the runner component 2 comprises a PCR amplification detection chamber 2-1-7 and a reaction chamber, and the reaction chamber comprises a nucleic acid extraction chamber 2-1-8 and a multifunctional chamber 2-1-9 which are communicated with each other.
In the structure, the liquid transfer bin 2-1-1 is used for uniformly mixing and transferring various liquid reagents and freeze-dried reagent balls; the reagent bin 2-1-2 is used for pre-packaging various liquid or solid reagents or is used as a reserved empty bin body; the sample bin 2-1-3 is used for adding a detection sample by a user; the micro flow channel 2-1-5 is used for liquid flow; the switching valve installation positions 2-1-6 are used for installing the flow channel switching valves 2-5 and the reagent switching valves 2-7; the PCR amplification detection bin 2-1-7 is used for carrying out amplification reaction and fluorescence signal detection; the nucleic acid extraction bin 2-1-8 is used for separating and extracting nucleic acid; the multifunctional bin 2-1-9 is used for heating liquid, uniformly mixing liquid, cracking nucleic acid, eluting nucleic acid and the like.
The micro-flow channel component with the structure is provided with an injection piston 2-4, a reagent switching valve 2-7, a flow channel switching valve 2-5 and a freeze-drying reagent 2-8; wherein, the freeze-dried reagent 2-8 is placed in a dry reagent bin of the reagent switching valve 2-7, the reagent switching valve 2-7 and the flow channel switching valve 2-5 are rotatably connected on a switching valve mounting position 2-1-6 on the micro-flow channel component, and the dry reagent bin can be communicated with a flow channel I2-1-10 and a flow channel II 2-1-11 on the surface of the micro-flow channel component by rotating the reagent switching valve 2-7; the channel rotary port 2-5-2 can be communicated with the selected micro channel 2-1-5 by rotating the channel switching valve 2-5.
The PCR microfluidic diagnosis chip can be operated in the following mode: when the injected sample enters the bottom of the 2-1-3 sample bin, the sample can be slowly injected to avoid bubble generation, then a piston rod is inserted into the bottom of the liquid transfer bin 2-1-1 and an injection piston 2-4 is assembled, then the rotary flow channel switching valve 2-5 and a reagent switching valve 2-7 are respectively driven, a flow channel rotary port 2-5-2 on the rotary flow channel switching valve 2-5 is communicated with the sample bin 2-1-3, a dry reagent bin filled with a freeze-dried magnetic bead reagent in the rotary reagent switching valve 2-7 is communicated with a flow channel I2-1-10 and a flow channel II 2-1-11, so that the liquid transfer bin 2-1-1, the flow channel I2-1-10, the dry reagent bin, the flow channel II 2-1-11, the injection piston rod and the injection piston, The annular common flow channel 2-5-1, the flow channel selection port 2-5-2 and the sample bin are communicated to 2-1-3, and a quantitative sample is sucked and mixed with a freeze-dried magnetic bead reagent through the matching of a piston rod and an injection piston 2-4 and reaches the liquid transfer bin 2-1-1; then the flow channel switching valve 2-5 is driven again to be communicated with a micro flow channel 2-1-5 corresponding to the reagent bin 2-1-2 in which the lysate is arranged, namely the liquid transfer bin 2-1-1 is communicated with the reagent bin 2-1-2 in which the lysate is arranged, and then the quantitative lysate is sucked to the liquid transfer bin 2-1-1 through the injection piston and is primarily mixed with the sample in the bin; then the flow channel switching valve 2-5 is driven again to be communicated with the nucleic acid extraction bin 2-1-8 and the multifunctional bin 2-1-8, the mixed liquid is discharged from the liquid transfer bin 2-1-1 into the multifunctional bin 2-1-9 by driving the injection piston 2-4, the outside of the multifunctional bin 2-1-9 is heated at constant temperature, and liquid can be sucked for many times by the injection piston 2-4 in the heating process to accelerate the cell lysis. After the cracking is finished, the nucleic acid in the liquid is combined with the magnetic beads, strong magnetism is generated by placing a permanent magnet or using an electromagnet outside the nucleic acid extraction bin 2-1-8, when the injection piston 2-4 is slowly transferred to the liquid transfer bin 2-1-1 from the liquid pumped from the inside of the multifunctional bin 2-1-9 and passes through the nucleic acid extraction bin 2-1-8, the magnetic beads in the liquid and the combination of the magnetic beads and the nucleic acid are adsorbed in the nucleic acid extraction bin 2-1-8; then, driving the flow channel switching valve 2-5 to be communicated with the sample bin 2-1-3 to discharge waste liquid, driving the flow channel switching valve 2-5 to be communicated with the reagent bin 2-1-2 internally provided with the lysate after the sample bin 2-1-3 is full, and discharging the residual waste liquid into the flow channel switching valve; then, continuously driving the flow channel switching valve 2-5 to communicate the reagent bin 2-1-2 with cleaning liquid in the reagent bin, sucking the liquid into the liquid transfer bin 2-1-1, then communicating the liquid transfer bin 2-1-1 with the nucleic acid extraction bin 2-1-8 through the driving flow channel switching valve 2-5, cleaning the magnetic beads in the nucleic acid extraction bin 2-1-8 for multiple times by using the cleaning liquid, then adsorbing the magnetic beads, and discharging waste liquid to the reagent bin 2-1-2 with the cleaning liquid in the reagent bin; then, absorbing the eluent in a reagent bin 2-1-2 with the quantitative eluent in the interior to elute the nucleic acid on the magnetic beads in a nucleic acid extraction bin 2-1-8, and separating the magnetic beads to obtain the eluent stored in a liquid transfer bin 2-1-1; then driving the flow channel switching valve 2-5 to be communicated with a micro flow channel 2-1-5 of a reserved empty chamber body in the reagent chamber 2-1-2, driving the reagent switching valve 2-7 to be communicated with a dry reagent chamber where a PCR reagent is located, enabling eluent and the PCR reagent to be fully mixed and then sucked into the liquid transfer chamber 2-1-1, finally driving the flow channel switching valve 2-5 to be communicated with the PCR amplification detection chamber 2-1-7, injecting mixed liquid in the liquid transfer chamber 2-1-1 into the PCR amplification detection chamber 2-1-7, sealing an inlet flow channel and an outlet flow channel of the PCR amplification detection chamber 2-1-7, simultaneously performing circulating temperature-changing heating on the PCR amplification detection chamber 2-1-7 through an external heating device by an instrument, and detecting a real-time fluorescence signal through an external fluorescence device, and finally, completing detection to obtain a result.
Furthermore, the upper part of the surface of the micro-channel component 2 is also provided with gas internal circulation holes 2-1-4, the gas internal circulation holes 2-1-4 are communicated with the micro-channel, and the micro-channel is communicated with the multifunctional bin 2-1-9. The multifunctional bin can be ventilated according to actual conditions in the structure, so that the application range of the whole PCR microfluidic diagnosis chip is enlarged.
Furthermore, the surface of the flow channel switching valve 2-5 is also provided with a ventilation flow channel 2-5-3, the micro flow channel connected with the gas circulation inner hole 2-1-4 is of a three-way type, the ventilation flow channel 2-5-3 can be communicated with the free end of the three-way type micro flow channel by rotating the flow channel switching valve 2-5, and the ventilation flow channel 2-5-3 is communicated with the PCR amplification detection bin 2-1-7 through the three-way type micro flow channel. The structure can realize the communication between the PCR amplification detection bin 2-1-7 and the atmosphere, when the flow channel is closed by the PCR amplification detection bin 2-1-7, the communication with the atmosphere can be realized through the rotary flow channel switching valve 2-5, whether the communication is realized or not can be selected according to the ventilation or not in practical situation, the atmosphere can be communicated, and other gases can be connected on the gas circulation inner hole 2-1-4 to increase the application range of the PCR microfluidic diagnosis chip.
Further, the micro-channel assembly 2 further comprises reagent sealing films 2-3, and the reagent sealing films 2-3 are arranged at the tops of the reagent bins 2-1-2. The reagent sealing film 2-3 is used for sealing practice, preventing the reagent from being polluted during assembly, and when the reagent in the reagent bin 2-1-2 is used, the corresponding reagent sealing film 2-3 of the puncture column can be used.
Further, the micro flow channel assembly 2 comprises a chip main body 2-1 and a flow channel sealing layer 2-6 which are assembled into a whole, wherein a plurality of cabin bodies and micro flow channels 2-1-5, a first flow channel 2-1-10 and a second flow channel 2-1-11 are arranged on the chip main body 2-1; the flow channel sealing layer 2-6 is provided with a reagent bin inlet 2-6-1 and a reagent bin outlet 2-6-2, the reagent bin inlet 2-6-1 is communicated with the flow channel I2-1-10, the reagent bin outlet 2-6-2 is communicated with the flow channel II 2-1-11, and the reagent switching valve 2-7 enables the dry reagent bin to be communicated with the reagent bin inlet 2-6-1 and the reagent bin outlet 2-6-2 through rotation. The above structure illustrates the specific structure of the micro flow channel assembly, and how the first flow channel 2-1-10 and the second flow channel 2-1-11 communicate with the dry reagent chamber.
Further, the chip main body 2-1 and the flow channel sealing layer 2-6 are combined together in any one of the connection modes of ultrasonic welding, bonding or hot melting to form a complete flow channel to form the micro-flow channel assembly.
Further, the PCR microfluidic diagnostic chip also comprises a chip upper shell assembly 1 arranged on the upper part of the microchannel assembly 2, wherein the chip upper shell assembly 1 comprises a chip upper shell 1-2, a chip sealing cover 1-1 is arranged on the chip upper shell 1-2, and the chip sealing cover 1-1 corresponds to the sample bin 2-1-3; and a sealing ring 2-2 is arranged between the chip upper shell component 1 and the micro flow channel component 2.
Furthermore, the chip upper shell assembly 1 further comprises a sealing elastic film 1-3 and a sealing film pressing plate 1-4, the sealing film pressing plate 1-4 is provided with a plurality of through holes, the through holes correspond to the reagent bins 2-1-2, and the sealing elastic film 1-3 is further arranged on the sealing film pressing plate 1-4.
When the chip is produced, various reagents required by the whole molecular diagnosis process are packaged in a reagent bin 2-1-2 and a reagent switching valve 2-7 arranged in a micro-channel assembly 2 in advance, the micro-channel assembly 2 is communicated with each functional unit through a micro-channel 2-1-5, an injection piston 2-4 for driving liquid to flow and a channel switching valve 2-5 for switching a channel are arranged, meanwhile, the micro-channel assembly 2 is also provided with an assembly chip shell assembly 1, a sealing elastic film 1-3, a chip sealing cover 1-1 and a sealing ring 2-2 between the sealing elastic film and the chip sealing cover are arranged on the chip shell assembly 1 and are all used for sealing the chip, so that the chip becomes a closed space, and the air in the chip forms internal circulation, thereby avoiding mutual pollution of the internal aerosol and the external aerosol of the chip. When the chip upper shell assembly 1 is arranged, when the chip is used, the puncture column is used for puncturing the reagent sealing film 2-3, the sealing elastic film 1-3 is separated, and the sealing elastic film 1-3 is not punctured, so that a good sealing effect can be achieved in the detection process.
Further, the PCR microfluidic diagnostic chip also comprises a chip lower shell 3 matched with the micro-channel assembly 2. The chip lower case 3 is used to assemble and seal the chip.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. The invention extends to any novel feature or any novel combination of features disclosed in this specification and any novel method or process steps or any novel combination of features disclosed.
Claims (10)
1. A full-automatic totally-enclosed fluorescence quantitative PCR microfluidic diagnosis chip is characterized by comprising a microchannel component, wherein a plurality of cabin bodies are arranged at the top and the lower part of the microchannel component, a plurality of microchannels are arranged on the surface of the microchannel component, and each microchannel is communicated with one cabin body; the middle part of the surface of the micro-channel component is also provided with a first channel and a second channel, the first channel is communicated with one bin body at the top, and an injection piston is arranged in the bin body;
the micro-channel assembly is also provided with a switching valve mounting position, and the switching valve mounting position is provided with a channel switching valve and a reagent switching valve which can rotate relative to the micro-channel assembly; a plurality of dry reagent bins are distributed on the reagent switching valve along the circumference, and the dry reagent bins can be communicated with the first flow channel and the second flow channel through rotation; the surface of the flow channel switching valve is provided with an annular common flow channel which is communicated with the second flow channel, the annular common flow channel is provided with a flow channel selection port which extends along the radial direction of the flow channel switching valve, and the flow channel rotation port can be communicated with any micro flow channel through rotation.
2. The fully-automatic fully-enclosed fluorescence quantitative PCR microfluidic diagnosis chip according to claim 1, wherein the cartridge body at the top of the flow channel assembly comprises a liquid transfer chamber, a plurality of reagent chambers and a sample chamber, and an injection piston is arranged in the liquid transfer chamber; the storehouse body of runner subassembly lower part includes PCR amplification detection storehouse, and reaction storehouse, the reaction storehouse is including nucleic acid extraction storehouse and the multi-functional storehouse of intercommunication each other.
3. The fully-automatic fully-enclosed fluorescence quantitative PCR microfluidic diagnosis chip according to claim 2, wherein the upper part of the surface of the microchannel assembly is further provided with a gas inner circulation hole, the gas inner circulation hole is communicated with a gas microchannel, and the gas microchannel is communicated with the multifunctional bin.
4. The fully automatic and fully enclosed fluorescence quantitative PCR microfluidic diagnosis chip according to claim 3, wherein the surface of the flow channel switching valve is further provided with a ventilation flow channel, the micro flow channel connected with the gas circulation inner hole is of a three-way type, the ventilation flow channel can be communicated with the free end of the three-way type micro flow channel by rotating the flow channel switching valve, and the ventilation flow channel is communicated with the PCR amplification detection chamber through the three-way type micro flow channel.
5. The fully-automatic and fully-enclosed fluorescence quantitative PCR microfluidic diagnosis chip according to claim 1, wherein the micro flow channel assembly further comprises a reagent sealing film, and the reagent sealing film is arranged on the tops of the plurality of reagent bins.
6. The fully-automatic fully-enclosed fluorescence quantitative PCR microfluidic diagnosis chip according to claim 1, wherein the microchannel assembly comprises a chip main body and a channel sealing layer which are assembled into a whole, the chip main body is provided with a plurality of cabin bodies and microchannels, a first channel and a second channel; the runner sealing layer is provided with a reagent bin inlet and a reagent bin outlet, the reagent bin inlet is communicated with the first runner, the reagent bin outlet is communicated with the second runner, and the reagent switching valve enables the dry reagent bin to be communicated with the reagent bin inlet and the reagent bin outlet through rotation.
7. The fully-automatic fully-enclosed fluorescence quantitative PCR microfluidic diagnosis chip according to claim 6, wherein the chip main body and the flow channel sealing layer are combined together by any one of ultrasonic welding, bonding or hot melting to form a complete flow channel to form a micro flow channel assembly.
8. The fully automatic and fully enclosed fluorescence quantitative PCR microfluidic diagnostic chip according to any one of claims 1 to 7, further comprising a chip upper shell assembly disposed on the upper portion of the microchannel assembly, wherein the chip upper shell assembly comprises a chip upper shell, a chip sealing cover is disposed on the chip upper shell, and the chip sealing cover corresponds to the sample chamber; and a sealing ring is arranged between the chip upper shell component and the micro-channel component.
9. The fully-automatic fully-enclosed fluorescence quantitative PCR microfluidic diagnostic chip according to claim 8, wherein the chip package assembly further comprises a sealing elastic film and a sealing film pressing plate, the sealing film pressing plate is provided with a plurality of through holes, the through holes correspond to the reagent chambers, and the sealing film pressing plate is further provided with a sealing elastic film.
10. The fully-automatic and fully-enclosed fluorescence quantitative PCR microfluidic diagnosis chip according to claim 9, further comprising a lower chip shell matched with the micro channel assembly.
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| CN202110383276.2A CN112934285A (en) | 2021-04-09 | 2021-04-09 | Full-automatic totally-enclosed fluorescence quantitative PCR microfluidic diagnosis chip |
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Cited By (7)
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| CN111826273A (en) * | 2020-07-22 | 2020-10-27 | 赵毅 | Automatic totally-enclosed micro-fluidic chip for nucleic acid detection |
| CN113667582A (en) * | 2021-08-17 | 2021-11-19 | 圣湘生物科技股份有限公司 | Nucleic acid detection device and nucleic acid detection method |
| CN113667598A (en) * | 2021-08-17 | 2021-11-19 | 圣湘生物科技股份有限公司 | Nucleic acid detection device and nucleic acid detection method |
| CN114100722A (en) * | 2021-12-27 | 2022-03-01 | 北京梓晶生物科技有限公司 | Portable full-automatic nucleic acid constant temperature amplification device |
| CN115678765A (en) * | 2022-11-07 | 2023-02-03 | 苏州思迈德生物科技有限公司 | Micro-fluidic chip suitable for molecular diagnosis |
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| WO2023071049A1 (en) * | 2021-11-01 | 2023-05-04 | 江苏汇先医药技术有限公司 | Vertical micro-fluidic chip and method for pcr test |
| CN114100722A (en) * | 2021-12-27 | 2022-03-01 | 北京梓晶生物科技有限公司 | Portable full-automatic nucleic acid constant temperature amplification device |
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