CN115595249A

CN115595249A - Multifunctional food safety bacterium detection system and detection method

Info

Publication number: CN115595249A
Application number: CN202211224525.4A
Authority: CN
Inventors: 王永丰; 丁妮
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-10-08
Filing date: 2022-10-08
Publication date: 2023-01-13

Abstract

The invention belongs to the technical field of food detection, and particularly relates to a multifunctional food safety bacteria detection system and a detection method, which comprise a detection casing, wherein an operation transparent box for providing a sterile operation space for workers is fixedly installed at one end in the detection casing, a reciprocating shaking assembly for rotationally shaking a culture dish is installed at the lower edge of a carrying turntable, a rotary driving mechanism for controlling the rotation of the carrying turntable and controlling the operation of the reciprocating shaking assembly is installed right below the center of a circle of the carrying turntable, a cell shaking separation mechanism for shaking the culture dish is fixedly installed right below an optical mirror mechanism below the carrying turntable, and a sterile gas space circulation mechanism for taking away the sterile gas in the operation box is assembled at the bottom of the operation transparent box. The invention can ensure that the cell density in each area of the culture solution is the same, reduce the influence of cell overlapping on detection work, and simultaneously ensure that the operation box is in a sterile environment for a long time.

Description

Multifunctional food safety bacterium detection system and detection method

Technical Field

The invention belongs to the technical field of food detection, and particularly relates to a multifunctional food safety bacterium detection system and a detection method.

Background

The food microorganism detection is a detection method which uses the theory and method of microbiology to detect the type, quantity, property and influence of microorganisms in food on human health so as to judge whether the food meets the quality standard, can effectively prevent or reduce the occurrence of food zoonosis, and ensure the physical health of people; the food microbial inspection is one of important indexes for measuring the sanitary quality of food and one of scientific bases for judging whether the food to be detected is eaten or not; the monitoring is performed by a bacteria detection device.

The problems existing in the prior art are as follows:

in the bacteria detection work, a plurality of sample diluents with different concentrations are generally configured to be used as reference, but when the detection work is performed, a great amount of time is spent on detecting bacteria in each culture dish, however, when one culture dish is detected, other culture dishes are in a waiting state, and in the long waiting process, the distribution of cells in the sample liquid in the culture dish changes, and as the cells sink to the bottommost part of the liquid and aggregate under the action of gravity along with the long standing process, the cell density at the bottom of the liquid becomes higher, the cell density at the upper half part of the cells becomes lower, and in the detection visual field, the total amount of the cells appearing in a detection area is larger than the actual condition, so that a larger error exists between the detection data and the actual condition, and the cell sinking and aggregation increase the possibility of cell stacking, and at this time, the detection work of a detector is directly influenced, so that the detector cannot determine the total amount of the cells in the area;

in addition, in the long-time process of stewing of culture dish, the standing time is overlength, other bacterium breeding's possibility will be bigger, but in the in-process of culture dish preparation, because the air in the preparation space is not circulated, consequently in the preparation process, the culture dish just contacts with other bacterium very easily, and in the follow-up waiting process that stews, other bacterium breeding's possibility will be bigger, and the breeding of other bacterium will directly influence the living environment of the inside cell of culture dish, and then probably increase the inside bacterial colony quantity of culture dish, direct influence testing result, consequently, need provide a culture dish preparation place that can be in aseptic condition for a long time here.

Disclosure of Invention

The invention aims to provide a multifunctional food safety bacterium detection system and a detection method, which can ensure that the cell densities in all areas of a culture solution are the same, reduce the influence of cell overlapping on detection work, and simultaneously enable an operation box to be in a sterile environment for a long time.

The technical scheme adopted by the invention is as follows:

a multifunctional food safety bacterium detection system comprises a detection shell, wherein an operation transparent box for providing a sterile operation space for workers is fixedly installed at one end inside the detection shell, and a detection mechanism for completing bacterium detection is fixedly installed at the other end inside the detection shell;

the detection mechanism comprises a detection machine body, cold air equipment is arranged inside the detection machine body, a cold air outlet used for conveying cold air is formed in the inner wall of the detection machine body, an optical mirror mechanism used for detecting bacteria is arranged inside the detection machine body, a carrying turntable is rotatably arranged inside the detection machine body, a vessel carrying disc used for placing a culture vessel is rotatably arranged in an annular array manner inside the carrying turntable, and an edge wheel is integrally arranged at the bottom edge of the vessel carrying disc;

a reciprocating shaking assembly for rotationally shaking the culture dish is arranged at the edge below the carrying rotary table, and a rotary driving mechanism for controlling the carrying rotary table to rotate and controlling the reciprocating shaking assembly to operate is arranged right below the center of the carrying rotary table;

the rotary driving mechanism comprises a rotary column which is rotatably installed at the bottom of the detection machine body and is spliced with the circular column, an inner cylinder is fixedly installed inside the rotary column, a spring telescopic tube is fixedly installed at the output end of the inner cylinder, a quincuncial insertion block which is movably inserted into the quincuncial groove is installed at the top end of the spring telescopic tube, a first helical gear and a first gear are fixedly installed at the upper end and the lower end of the outer surface of the rotary column respectively, a main motor is fixedly installed inside the detection machine body and located on one side of the rotary column, and a second helical gear meshed with the first helical gear is fixedly installed at the output end of the main motor;

the reciprocating shaking assembly comprises a first side rotating column, a second side rotating column and a third side rotating column which are rotatably installed at the bottom of the detection machine body, the first side rotating column, the second side rotating column and the third side rotating column are located on the same straight line, a second gear meshed with the first gear is fixedly installed at the top end of the first side rotating column, the first side rotating column and the second side rotating column are in transmission connection through a belt, a rotary disc is fixedly installed at the top end of the second side rotating column, an eccentric rod is fixedly connected to the edge of the upper surface of the rotary disc, a top rod is fixedly connected to the top end of the third side rotating column, a laminating arc-shaped block used for being in contact with an edge wheel is fixedly connected to the tail end of the top rod, a straight groove is formed in the lower surface of the top rod, and the eccentric rod is movably inserted into the straight groove;

and a cell vibration separation mechanism for vibrating a culture dish is fixedly arranged under the object carrying turntable and is positioned under the optical lens mechanism.

Cell vibrations separating mechanism includes the cylinder, the output fixed mounting of cylinder has the main stage body, the top of the main stage body is rotated and is installed screw rod one, just the top one end fixed mounting of the main stage body has and is used for making a rotatory motor of screw rod one, the top of the main stage body is passed through pipe guide rod subassembly straight line and is slided the equipment and has been assisted the stage body, just it is fixed to be provided with the screwed pipe one with a screw rod spiro union to assist stage body bottom.

The top of assisting the stage body is rotated and is installed screw rod two, just the top one end fixed mounting of assisting the stage body has and is used for making two rotatory motors of screw rod two, the top of assisting the stage body has the balladeur train through the equipment of pipe guide rod subassembly sharp slip, just the fixed solenoid two that are provided with two spiro unions of screw rod in balladeur train bottom, balladeur train top fixed mounting has the mini electromagnetic shaker that is used for vibrations household utensils to carry dish.

The operation transparent case includes the control box of making by transparent plastic board, the operation mouth has all been seted up at the outer wall bottom both ends of control box, the household utensils groove that is used for placing the culture dish is seted up to control box bottom inner wall equidistance, just warning light one is all installed to one side in household utensils groove, the one end fixed mounting of control box one side inner wall has the test-tube rack that is used for placing the test tube, just warning light two is installed to test-tube rack bottom outer wall equidistance, the mouth of shaking has been seted up to the one end of control box bottom inner wall, the oscillator that is used for shaking sample and normal saline mixed liquid is seted up under the control box bottom position is in the mouth of shaking.

The other end of the inner wall of one side of the operation box is fixedly provided with an operation prompter for prompting the detection step, and the operation prompter consists of a display, a touch screen input assembly and a sequence prompting system; and a detection step input module for inputting various detection work detection steps and a detection step prompt module for prompting the detection steps through a prompt lamp are arranged in the sequence prompt system.

The detection step input module is internally provided with a step sequence input module and a corresponding light selection module for prompting the selection of a designated culture dish; the inside step order display module that will operate step through the display demonstration one by one that is provided with of detection step suggestion module, be used for lightening the suggestion lamp linkage module of appointed culture dish or near test tube suggestion lamp and be used for touching the display alright carry out the touch screen trigger module on next step of operation.

The utility model discloses a normal saline flow control device, including operation box, drain pipe top fixed mounting has a control valve that is used for flow control, the top one end fixed mounting of operation box has the normal saline case, just normal saline bottom of the case portion is connected with the drain pipe that extends to the operation box inside, drain pipe top fixed mounting has.

The bottom equipment of operation transparent case has and is used for taking away the inside aseptic gas space circulation mechanism who has the fungus gas of control box, aseptic gas space circulation mechanism includes that the equidistance sets up the air pump body in the control box bottom, control box bottom one end fixed mounting has driving motor, just driving motor output end fixed mounting has the working shaft that is used for driving all air pump bodies and works together, the output of the gas pump body has the purge tube and is connected with the contact tube through pipe connection, the purification filter core is all installed to the inside of purge tube.

A bottom suction pipe is fixedly installed on one side of the bottom of the operation box, the top of the bottom suction pipe is connected with air suction branch pipes extending to the inside of the operation box, the distance between every two adjacent air suction branch pipes is gradually increased from the middle to the two sides, the bottom of the bottom suction pipe is connected with a curved conduit at equal intervals, the tail end of the curved conduit is connected and communicated with a purification pipe, and the bottommost part of the curved section of the curved conduit is connected with an impurity discharge pipe for discharging impurities; the top of the inner wall of one side of the operation box is fixedly provided with a top air pipe, the side wall of the top air pipe is connected with air outlet branch pipes parallel to the horizontal plane at equal intervals, and the tail ends of the air outlet pipes are connected and communicated with the top air pipe.

A multifunctional food safety bacterium detection method comprises the following specific steps:

s1: sterile gas space circulation mechanism can make the inside sterile environment that is in for a long time of control box, and the inspector accomplishes the preparation work to the detection in the control box, injects into through the drain pipe normal saline in the sample, carries out the oscillation through the oscillator with sample and normal saline and mixes, alright obtain the detection sample liquid of initial dilution this moment.

S2: extracting primary diluent into two culture dishes according to an operation flow displayed by an operation prompter, placing the two culture dishes into a dish groove corresponding to the two culture dishes according to a correspondingly lightened prompt lamp I, then touching an operation prompter screen to display the next operation step, taking down test tubes at corresponding positions in a test tube rack according to a lightened prompt lamp II, injecting physiological saline into the test tubes, extracting the primary diluent and injecting the primary diluent into the test tubes, finally obtaining secondary diluent, touching the operation prompter screen again, extracting the secondary diluent in the test tubes into the other two culture dishes according to the lightened prompt lamp I, and placing the culture dishes into other dish grooves corresponding to the lightened prompt lamp I; according to the preset preparation steps, the configuration of each group of liquid is completed, and the liquid is placed into the corresponding position according to the prompt of the corresponding prompt lamp.

S3: according to the detection sequence, the corresponding culture dishes are taken down and sequentially placed into the corresponding vessel carrying discs, then the inner cylinder is started to enable the rotary column and the central column to be in a connection state, the main motor is started to enable the rotary column to rotate with the carrying rotary disc, and the culture dishes in the first vessel carrying disc are sent to the position right below the optical lens mechanism to be detected.

S4: in the process of first culture dish transportation, carry the laminating arc piece of thing carousel bottom and will reciprocate simultaneously to rotate, carry the dish with the help of the friction between laminating arc piece and the edge wheel to drive the household utensils that corresponds and begin to rotate, the culture dish that first household utensils carried the dish inside at this moment also can correspond the rotation and rock to this cell that avoids in the culture dish sinks the gathering.

S5: at the in-process that first culture dish detected, in order to guarantee that the inside cell of other culture dishes can not sink the gathering, the plum blossom inserted block is withdrawed to control interior cylinder this moment, makes main motor continue work again, and the rotary column at this moment can not take and carry the thing carousel rotation, but the rotary column can drive the reciprocal rotation of laminating arc piece, and then drives the reciprocal rotation of the household utensils year dish of other positions, and then the culture dish except that first culture dish will be rotatory simultaneously.

S6: when the cells are overlapped at the beginning of detection, moving the mini-vibrator to the position where the overlapped cells exist, starting the mini-vibrator when the mini-vibrator abuts against the lower surface of the corresponding vessel carrying disc, vibrating the liquid in the culture vessel through the mini-vibrator, and starting the cell detection when the cells are not overlapped.

The invention has the technical effects that:

(1) In the process of conveying the vessel carrying disc by the carrying turntable, the ejector rod rotates in a reciprocating manner by taking the side rotating column III as a circle center, the corresponding vessel carrying disc is driven to rotate in a reciprocating manner by virtue of friction force between the attaching arc-shaped block and the edge wheel, therefore, when the first vessel carrying disc is conveyed, the culture vessel in the first vessel carrying disc correspondingly rotates in a reciprocating manner, the culture solution in the corresponding vessel can shake, cells in the culture solution can simultaneously displace, the position of the cells can also change under the action of the liquid shaking, the condition that the cells in the culture vessel gather and sink is avoided, the possibility that the cells overlap due to the sinking movement is reduced, the cells are suspended in the culture solution as uniformly as possible, the cells are guaranteed to be normally distributed in the culture solution, the cell density in each region of the culture solution is guaranteed to be the same, the problem of large difference of regional density is solved, the detection error is reduced, the data accuracy is improved, and the influence of the cell overlapping on the detection work can be reduced.

(2) According to the invention, the inner cylinder is started to insert the plum blossom insert block into the plum blossom groove, the rotary column and the circle center column are in a connection state, otherwise, when the inner cylinder is controlled to retract the plum blossom insert block, the rotary column and the circle center column are disconnected, the carrying rotary disc is not driven by the rotary column to rotate, the attaching arc block can continue to rotate in a reciprocating manner, other culture dishes can rotate and rock in the detection process, and cells in other culture dishes can be kept in a good distribution condition in the waiting process.

(3) According to the invention, when cell overlapping is found during detection, the mini-vibrator is controlled to move and aim at the area where the overlapped cells occur, then the mini-vibrator is started immediately, the liquid in the culture dish is vibrated by the mini-vibrator, and at the moment, the cells aggregated in the culture solution are separated under the action of vibration, so that the problem of cell aggregation occurring during detection is solved, the problem that the cell aggregation cannot be processed during detection is solved, the detection work is more flexible, and when no cell overlapping occurs, the detection work of the cells is started.

(4) In each detection work, different culture dishes are placed in an appointed position in advance for temporary storage, when the step is carried out to a certain step, the corresponding prompt lamp I or the corresponding prompt lamp II can be lightened, so that the prompt function is achieved, the preparation work is carried out according to the prompt mode, the prompt mode is suitable for the detection work of multiple samples in a large batch at one time, the process that a person continuously searches for multiple culture dishes in different steps can be omitted, the situation that the person takes the samples wrongly due to visual fatigue is reduced, the step-by-step prompt effect can also enable the detection work to be carried out according to the appointed step sequence, and the situation that the person makes mistakes in the steps can also be reduced.

(5) According to the invention, air in the operation box is pumped out from the bottom of one end and is finally sent into the operation box again from the top of the other end, the ventilation work is continuously carried out, the air in the operation box is continuously replaced, the bacteria-containing gas in the operation box is immediately pumped out in the continuous purification and replacement process, and the sterile gas environment in the operation box is maintained as long as possible through the continuous ventilation work, so that the residence time of other bacteria in the operation box is greatly reduced, the possibility of bacteria nidating in the culture dish is further reduced, and the influence of other bacteria on the detection work is reduced as much as possible.

Drawings

FIG. 1 is an internal block diagram of a test housing provided by an embodiment of the present invention;

FIG. 2 is a side view block diagram of an operating transparent box provided by an embodiment of the present invention;

FIG. 3 is an internal structural view of an operation transparent box provided by the embodiment of the present invention;

FIG. 4 is a diagram of an internally integrated system for operating a prompter provided by an embodiment of the present invention;

FIG. 5 is a block diagram of a sterile gas space circulation mechanism provided by an embodiment of the present invention;

FIG. 6 is a block diagram of a detection mechanism provided by an embodiment of the present invention;

fig. 7 is a front view structural view of a carrier turntable provided in the embodiment of the present invention;

FIG. 8 is a bottom block diagram of a carrier carousel provided in an embodiment of the present invention;

FIG. 9 is a block diagram of a single conformable arcuate block drive provided by an embodiment of the present invention;

fig. 10 is an internal structural view of a rotary column provided in an embodiment of the present invention;

FIG. 11 is a structural diagram of a cell shaking separation mechanism provided in an embodiment of the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

1. detecting the shell; 2. operating the transparent box; 201. an operation box; 202. an operation port; 203. a vessel trough; 204. a first prompt lamp; 205. a test tube rack; 206. a second prompting lamp; 207. an oscillation port; 3. operating a prompter; 301. a display; 302. a touch screen input assembly; 303. a sequential cue system; 304. an input module in the detection step; 305. a detection step prompting module; 306. a step sequence input module; 307. a corresponding light selection module; 308. a step sequence display module; 309. a cue light linkage module; 310. next, a touch screen triggering module is touched; 4. an oscillator; 5. a physiological saline tank; 501. a liquid outlet pipe; 502. a control valve; 6. a sterile gas space circulation mechanism; 601. a bottom straw; 602. a suction branch pipe; 603. a curved catheter; 604. a trash removal pipe; 605. a purge tube; 606. a gas pump body; 607. a drive motor; 608. a working shaft; 609. a delivery pipe; 610. a gas ejecting pipe; 611. an air outlet branch pipe; 7. a detection mechanism; 701. detecting a body; 702. a cold air outlet; 703. an optical mirror mechanism; 704. a loading turntable; 705. a vessel carrying tray; 706. an edge wheel; 707. a cylindrical core; 708. turning the column; 709. a first bevel gear; 710. a main motor; 711. a first gear; 712. a first side turning column; 713. a second gear; 714. a second side turning column; 715. a belt; 716. a turntable; 717. an eccentric rod; 718. a third side turning column; 719. a top rod; 720. a straight groove; 721. fitting the arc-shaped block; 722. a plum blossom groove; 723. an inner cylinder; 724. a spring extension tube; 725. a quincuncial insertion block; 8. a cell vibration separation mechanism; 801. a cylinder; 802. a main stage body; 803. a first screw rod; 804. a first motor; 805. an auxiliary table body; 806. a second screw; 807. a second motor; 808. a carriage; 809. a mini vibrator.

Detailed Description

In order that the objects and advantages of the invention will be more clearly understood, the following description is given in conjunction with the accompanying examples. It is to be understood that the following text is merely illustrative of one or more specific embodiments of the invention and does not strictly limit the scope of the invention as specifically claimed.

As shown in fig. 1-11, a multifunctional food safety bacteria detection system comprises a detection case 1, an operation transparent box 2 for providing a sterile operation space for workers is fixedly installed at one end inside the detection case 1, and a detection mechanism 7 for completing bacteria detection is fixedly installed at the other end inside the detection case 1.

The first embodiment is as follows:

referring to fig. 6 and 7, the detecting mechanism 7 includes a detecting body 701, a cold air device is installed inside the detecting body 701, a cold air outlet 702 for conveying cold air is formed in an inner wall of the detecting body 701, an optical mirror mechanism 703 for detecting bacteria is installed inside the detecting body 701, a carrying turntable 704 is rotatably installed inside the detecting body 701, a vessel carrying tray 705 for placing a culture vessel is rotatably installed inside the carrying turntable 704 in an annular array manner, and an edge wheel 706 is integrally arranged at a bottom edge of the vessel carrying tray 705;

according to the structure, the culture dish is placed in the dish carrying disc 705 on the surface of the carrying rotary disc 704, the detection of the microorganism is completed through the optical lens mechanism 703, and the cold air outlet 702 is used for conveying cold air in the detection process so as to reduce the possibility of microorganism breeding.

Referring to fig. 7 and 8, a reciprocating shaking assembly for rotationally shaking the culture dish is installed at the lower edge of the object carrying turntable 704, and a rotary driving mechanism for controlling the object carrying turntable 704 to rotate and controlling the reciprocating shaking assembly to operate is installed right below the center of the object carrying turntable 704;

referring to fig. 9 and 10, a center of a circle 707 is fixedly connected to a center of a circle of a lower surface of the object carrying turntable 704, a quincunx groove 722 is formed at a tail end of the center of the circle 707, the rotation driving mechanism includes a rotating column 708 rotatably installed at the bottom of the detection machine body 701, the rotating column 708 is inserted into the center of the circle 707, an internal cylinder 723 is fixedly installed inside the rotating column 708, an output end of the internal cylinder 723 is fixedly installed with a spring extension tube 724, a quincunx insertion block 725 movably inserted into the quincunx groove 722 is installed at a top end of the spring extension tube 724, a helical gear 709 and a gear 711 are fixedly installed at upper and lower ends of an outer surface of the rotating column 708 respectively, a main motor 710 is fixedly installed inside the detection machine body 701 at one side of the rotating column 708, and a helical gear ii engaged with the helical gear 709 is fixedly installed at an output end of the main motor 710;

according to the structure, the internal cylinder 723 is started, the internal cylinder works to drive the spring telescopic tube 724 and the quincuncial inserting block 725 at the top end to move upwards together, when the quincuncial inserting block 725 is inserted into the quincuncial groove 722, the rotating column 708 and the circle center column 707 are in a connected state, then the main motor 710 is started immediately, works to enable the second bevel gear to rotate, and drives the rotating column 708 and the carrying turntable 704 to rotate together through the meshing of the first bevel gear 709 and the second bevel gear; in addition, when the internal cylinder 723 is controlled to retract the quincuncial plug block 725, the rotating column 708 is disconnected from the circle center column 707, then the main motor 710 continues to work, and the rotating column 708 starts to rotate again but does not carry the carrying turntable 704 to rotate;

referring to fig. 8 and 9, the reciprocating shaking assembly includes a first side rotation column 712, a second side rotation column 714 and a third side rotation column 718 rotatably installed at the bottom of the detection body 701, the first side rotation column 712, the second side rotation column 714 and the third side rotation column 718 are located on the same straight line, a second gear 713 engaged with the first gear 711 is fixedly installed at the top end of the first side rotation column 712, the first side rotation column 712 and the second side rotation column 714 are in transmission connection through a belt 715, a rotary disc 716 is fixedly installed at the top end of the second side rotation column 714, an eccentric rod 717 is fixedly connected to the edge of the upper surface of the rotary disc 716, a top rod 719 is fixedly connected to the top end of the third side rotation column 718, an attaching arc-shaped block 721 for contacting with the edge wheel 706 is fixedly connected to the end of the top rod 719, a straight groove 720 is formed in the lower surface of the top rod 719, and the eccentric rod 717 is movably inserted into the straight groove 720.

According to the structure, the side rotary column II 714 and the rotary table 716 at the top end of the side rotary column II can be finally driven to rotate together through the meshing of the gear I711 and the gear II 713 and the transmission of the belt 715, at the moment, the eccentric rod 717 on the surface of the rotary table 716 relatively moves between the straight groove 720 and the ejector rod 719 and simultaneously drives the ejector rod 719 to rotate in a reciprocating manner around the side rotary column III 718, the corresponding vessel carrying disc 705 can be driven to rotate in a reciprocating manner through the friction force between the attaching arc-shaped block 721 and the edge wheel 706 while the attaching arc-shaped block 721 reciprocates, a culture vessel inside the vessel carrying disc 705 also rotates in a reciprocating manner, the culture solution inside the vessel carrying disc 705 shakes, cells in the culture solution also displace in the reciprocating manner, the position of the cells in the culture vessel is changed under the action of the shaking of the liquid, the condition that the cells in the culture vessel are gathered and sink is avoided, the possibility that a plurality of cells are overlapped in the sinking motion is reduced, the cells are suspended in the culture solution as uniformly as possible, the good observation field of view for detecting the cells is ensured, and the detection error is reduced.

The working principle of the invention is as follows: placing the culture dishes into the corresponding vessel carrying discs 705 in sequence, starting an inner air cylinder 723, working to drive a spring telescopic tube 724 and a quincuncial insert block 725 at the top end to move upwards together, when the quincuncial insert block 725 is inserted into a quincuncial groove 722, enabling a rotating column 708 and a circle center column 707 to be in a connected state, immediately starting a main motor 710, working to enable a helical gear II to rotate, driving the rotating column 708 and a carrying turntable 704 to rotate together through meshing of a helical gear I709 and the helical gear II, sending the culture dish in the first vessel carrying disc 705 to be right below an optical lens mechanism 703 for detection, in the process of transporting the first culture dish, finally driving a side rotating column II 714 to rotate together with a rotating disc 716 at the top end through meshing of a gear I711 and a gear II 713 and transmission of a belt 715, driving an eccentric rod on the surface of the rotating column II to move relatively between the straight groove 720 and a push rod 719 at the same time, driving the push rod 717 on the surface of the rotating disc 716 to rotate relative to the push rod 717 and simultaneously drive the push rod vessel to rotate with the side rotating column III 718 as a circle center, and simultaneously driving a reciprocating force of the reciprocating wheel 705 and the corresponding arc carrying disc 721 to rotate so as to drive a corresponding cell carrying disc 706 to rotate, and avoid cell collecting cells in the culture dish 706, and shaking of the culture dish 706;

in the process of detecting the first culture dish, in order to ensure that cells in other culture dishes do not gather and sink due to overlong waiting time, at this time, the internal cylinder 723 is controlled to retract the quincuncial plug block 725, so that the rotating column 708 is separated from the central column 707, then the main motor 710 continues to work, at this time, the rotating column 708 starts to rotate again but does not rotate with the carrying rotary disk 704, therefore, all the attaching arc-shaped blocks 721 rotate back and forth, and further the dish carrying disks 705 at other positions rotate back and forth, at this time, other culture dishes except the first culture dish continuously rotate back and forth before detection, so as to ensure that the cells in each culture dish do not gather and sink.

Example two:

referring to fig. 7, a cell shaking separation mechanism 8 for shaking the culture dish is fixedly installed below the objective table 704 and directly below the optical mirror mechanism 703.

Referring to fig. 11, the cell vibration separation mechanism 8 includes a cylinder 801, a main stage 802 is fixedly installed at an output end of the cylinder 801, a first screw 803 is rotatably installed above the main stage 802, a first motor 804 for rotating the first screw 803 is fixedly installed at one end above the main stage 802, a first auxiliary stage 805 is linearly slidably assembled above the main stage 802 through a guide pipe guide rod assembly, and a first solenoid screwed with the first screw 803 is fixedly installed at a bottom of the first auxiliary stage 805.

According to the above structure, the first motor 804 is activated to rotate the first screw 803, and the second table 805 is moved linearly by the guide rod assembly.

Referring to fig. 11, a second screw 806 is rotatably mounted above the auxiliary table 805, a second motor 807 for rotating the second screw 806 is fixedly mounted at one end above the auxiliary table 805, a second screw 808 is linearly slidably mounted above the auxiliary table 805 through a guide pipe guide rod assembly, a second screw pipe screwed with the second screw 806 is fixedly arranged at the bottom of the second slide 808, and a mini vibrator 809 for vibrating the vessel carrying plate 705 is fixedly mounted at the top end of the second slide 808.

According to the structure, the second screw 806 is rotated by the second motor 807, the sliding frame 808 moves linearly under the action of the guide rod assembly of the guide pipe, the first screw 803 and the second screw 806 are vertically arranged, so that the position of the mini-vibrator 809 on the horizontal plane can be changed, after the mini-vibrator 809 aligns at the occurrence area of overlapped cells, the cylinder 801 is started immediately to enable the top end of the mini-vibrator 809 to abut against the lower surface of the corresponding vessel carrying disc 705, then the mini-vibrator 809 is started, the liquid in the culture vessel is vibrated by the mini-vibrator 809, at the moment, the cells gathered in the culture liquid are separated under the action of vibration, and therefore the problem of cell gathering in the detection work is solved, the problem that the cells cannot be gathered in the detection work is solved, the detection work is more flexible, and the cell detection work is started when the cells are not overlapped.

The working principle of the invention is as follows: in the detection work by using the optical mirror mechanism 703, when cells are found to be overlapped in the visual field and the observation visual field is seriously affected, at this time, according to the position of the detection area, the corresponding first motor 804 and second motor 807 are started, the position of the mini-vibrator 809 on the horizontal plane is adjusted to be aligned with the generation area of the overlapped cells, then the cylinder 801 enables the mini-vibrator 809 to move upwards until the top end of the mini-vibrator 809 abuts against the lower surface of the corresponding vessel carrying disc 705, then the mini-vibrator 809 is started, the liquid in the culture vessel is vibrated by the mini-vibrator 809, at this time, the cells aggregated in the culture solution are separated under the vibration effect, and when no cell overlap occurs, the cell detection work is started.

Example three:

referring to fig. 2, the operation transparent box 2 includes the operation box 201 made by transparent plastic board, operation mouth 202 has all been seted up at the outer wall bottom both ends of operation box 201, the household utensils groove 203 that is used for placing the culture dish is seted up to operation box 201 bottom inner wall equidistance, and a prompt lamp 204 is all installed to one side of household utensils groove 203, the one end fixed mounting of operation box 201 one side inner wall has a test-tube rack 205 that is used for placing the test tube, and the outer wall equidistance is installed to test-tube rack 205 bottom outer wall and has a prompt lamp two 206, oscillation mouth 207 has been seted up to the one end of operation box 201 bottom inner wall, oscillator 4 that is used for vibrating sample and normal saline mixed liquid is seted up to operation box 201 bottom under being located oscillation mouth 207.

According to the structure, the operation port 202 is used for providing a space for the arm of the inspector to pass through, and the arm of the inspector extends into the operation box 201 after the inspector, so that the entering amount of bacteria can be reduced, and a sterile environment is provided for the inspector to work as far as possible; the examiner can insert the bag containing the mixed liquid of the sample and the physiological saline from the shaking port 207 into the inside of the shaker 4, and shake the sample bag by the shaker 4, thereby performing the sample dilution work.

Referring to fig. 2, a saline tank 5 is fixedly installed at one end of the top of an operation box 201, a liquid outlet pipe 501 extending into the operation box 201 is connected to the bottom of the saline tank 5, and a control valve 502 for controlling flow rate is fixedly installed at the top end of the liquid outlet pipe 501.

According to the above configuration, the control valve 502 can control the flow of a predetermined amount of physiological saline from the physiological saline tank 5 by a predetermined amount of the required physiological saline.

Referring to fig. 2 and 4, an operation prompter 3 for prompting the detection step is fixedly mounted at the other end of the inner wall of one side of the operation box 201, and the operation prompter 3 is composed of a display 301, a touch screen input assembly 302 and a sequence prompting system 303; the sequence prompt system 303 is internally provided with a detection step input module 304 for inputting various detection work detection steps and a detection step prompt module 305 for prompting the detection steps through a prompt lamp.

Referring to fig. 4, the detection step input module 304 is internally provided with a step sequence input module 306 and a corresponding light selection module 307 for prompting selection of a designated culture dish; the detection step prompting module 305 is internally provided with a step sequence display module 308 for displaying the operation steps one by one through the display 301, a prompting lamp linkage module 309 for lighting a prompting lamp near a designated culture dish or test tube, and a next touch screen triggering module 310 for performing the next operation by touching the display 301.

According to the structure, in each detection work, different culture dishes are placed in designated positions in advance for temporary storage, when the step is carried out to a certain step, the corresponding first prompt lamp 204 or the second prompt lamp 206 can be lightened, so that the prompt function is achieved, preparation work is carried out according to the prompt mode, the prompt mode is suitable for detection work of multiple samples in a large batch at one time, the process that the samples are continuously searched in the culture dishes in different steps by manpower can be omitted, the situation that the samples are taken wrongly due to visual fatigue is reduced, the detection work can be carried out according to the designated step sequence by means of the step prompt effect, and the situation that an inspector makes mistakes in the steps can be reduced.

The working principle of the invention is as follows: in the process of debugging the equipment, step information of various detection works and the appointed placing positions of different culture dishes in each step are preferably input in advance through the detection step input module 304, then when a bacterium detection work is carried out, the detection step prompting module 305 can remind an inspector of which corresponding culture dish needs to be taken in a certain step according to step prompting, and after one step is completed, the corresponding culture dish is immediately jumped to a page of the next step under the action of the next touch screen triggering module 310 through the touch display 301, and meanwhile, a corresponding prompting lamp in the step is lightened;

for example, when two kinds of dilution liquids with concentrations of 1.

Example four:

referring to fig. 3 and 5, the bottom of the operation transparent box 2 is assembled with a sterile air space circulation mechanism 6 for taking away the bacteria-containing air inside the operation box 201, the sterile air space circulation mechanism 6 comprises air pump bodies 606 which are equidistantly arranged at the bottom of the operation box 201, one end of the bottom of the operation box 201 is fixedly provided with a driving motor 607, an output end of the driving motor 607 is fixedly provided with a working shaft 608 for driving all the air pump bodies 606 to work together, an output end of the air pump body 606 is connected with a purification tube 605 and a delivery tube 609 through a pipeline, and the purification filter element is installed inside the purification tube 605.

According to the above structure, the driving motor 607 works continuously, and the working shaft 608 is driven to rotate by the work of the driving motor 607, and the air pump body 606 starts to operate and finishes the work of air suction, at this time, the air inside the operation box 201 is sucked into the bottom suction pipe 601 from the bottom of one end through each air suction branch pipe 602, and then enters the purification pipe 605, and the filter element inside the purification pipe 605 can perform sterile filtration treatment on the air.

Referring to fig. 3 and 5, a bottom suction pipe 601 is fixedly installed at one side of the bottom of the operation box 201, the top of the bottom suction pipe 601 is connected with a suction branch pipe 602 extending to the inside of the operation box 201, the distance between adjacent suction branch pipes 602 is gradually increased from the middle to two sides, the bottom of the bottom suction pipe 601 is connected with a curved conduit 603 at equal intervals, the tail end of the curved conduit 603 is connected and communicated with a purification pipe 605, and the bottommost part of the curved section of the curved conduit 603 is connected with an impurity discharge pipe 604 for discharging impurities; the top of the inner wall of one side of the operation box 201 is fixedly provided with a top air pipe 610, the side wall of the top air pipe 610 is equidistantly connected with air outlet branch pipes 611 parallel to the horizontal plane, and the tail ends of the air outlet pipes 609 are communicated with the top air pipe 610.

According to the structure, the filtered gas enters the top gas pipe 610 through the guide pipe 609 again, and finally enters the operation box 201 through the gas outlet branch pipes 611 at the top of the other end inside the operation box 201 again, the ventilation work is continuously performed, the air inside the operation box 201 is continuously replaced, in the process of continuous purification and replacement, the bacteria-containing gas inside the operation box 201 can be immediately pumped away, the sterile gas environment inside the operation box 201 is maintained through continuous ventilation work as long as possible, the residence time of other bacteria in the operation box 201 is greatly reduced, and the influence of other bacteria on the detection work is reduced as much as possible.

The working principle of the invention is as follows: when an inspector operates in the operation box 201, the driving motor 607 will work together to drive the working shaft 608 to rotate and to make the air pump body 606 start to operate and finish the air extraction, the air in the operation box 201 will be sucked into the bottom suction pipe 601 from the bottom of one end through each air suction branch pipe 602, and after being sterile-filtered by the filter element in the purification pipe 605, will enter the top air pipe 610 from the delivery pipe 609 again, and finally enter the operation box 201 again from each air outlet branch pipe 611 at the top of the other end in the operation box 201, and this ventilation operation is performed continuously, so that the air in the operation box 201 will be replaced continuously, and in the process of continuous purification and replacement, the sterile air environment in the operation box 201 is maintained as long as possible.

s1: the sterile gas space circulation mechanism 6 can keep the inside of the operation box 201 in a sterile environment for a long time, an inspector completes preparation work for detection in the operation box 201, physiological saline is injected into a sample through the liquid outlet pipe 501, then the sample and the physiological saline are subjected to oscillation mixing through the oscillator 4, and then the primarily diluted detection sample liquid can be obtained.

S2: extracting primary diluent into two culture dishes according to an operation flow displayed by an operation prompter 3, placing the two culture dishes into a corresponding dish groove 203 according to a corresponding lightened prompt lamp I204, then touching a screen of the operation prompter 3 to display the next operation step, taking down test tubes at corresponding positions in a test tube rack 205 according to a lightened prompt lamp II 206, injecting physiological saline into the test tubes, extracting the primary diluent and injecting the primary diluent into the test tubes, finally obtaining secondary diluent, touching the screen of the operation prompter 3 again, extracting the secondary diluent in the test tubes into the other two culture dishes according to the lightened prompt lamp I204, and placing the culture dishes into other dish grooves 203 corresponding to the lightened prompt lamp I204; according to the preset preparation steps, the configuration of each group of liquid is completed, and the liquid is placed into the corresponding position according to the prompt of the corresponding prompt lamp.

S3: according to the detection sequence, the corresponding culture dishes are taken down and placed in the corresponding vessel carrying trays 705 in sequence, then the inner cylinder 723 is started to enable the rotating column 708 and the circle center column 707 to be in a connection state, at the moment, the main motor 710 is started to enable the rotating column 708 to rotate with the carrying turntable 704, and the culture dishes in the first vessel carrying tray 705 are sent to the position right below the optical lens mechanism 703 for detection.

S4: in the process of transporting the first culture dish, the attaching arc-shaped block 721 at the bottom of the carrying turntable 704 rotates reciprocally at the same time, the corresponding vessel carrying tray 705 is driven to rotate by virtue of the friction between the attaching arc-shaped block 721 and the edge wheel 706, and the culture dish inside the first vessel carrying tray 705 correspondingly rotates and shakes at the moment, so that the cells in the culture dish are prevented from sinking and gathering.

S5: in the process of detecting the first culture dish, in order to ensure that cells in other culture dishes do not sink and gather, the internal cylinder 723 is controlled to withdraw the plum blossom insert 725, the main motor 710 continues to work, the rotating column 708 does not drive the carrying turntable 704 to rotate at the moment, but the rotating column 708 drives the attaching arc block 721 to rotate in a reciprocating manner, so that the vessel carrying discs 705 at other positions are driven to rotate in a reciprocating manner, and the culture dishes except the first culture dish rotate at the same time.

S6: when the cells are overlapped at the beginning of detection, the mini-vibrator 809 is moved to the position where the overlapped cells exist, when the mini-vibrator 809 abuts against the lower surface of the corresponding vessel carrying tray 705, the mini-vibrator 809 is started, the liquid in the culture vessel is vibrated through the mini-vibrator 809, and when the cells are not overlapped, the detection of the cells is started.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention. Structures, devices, and methods of operation not specifically described or illustrated herein are generally practiced in the art without specific recitation or limitation.

Claims

1. The utility model provides a multi-functional food security bacterium detecting system, is including detecting casing (1), its characterized in that: an operation transparent box (2) used for providing a sterile operation space for workers is fixedly installed at one end inside the detection case (1), and a detection mechanism (7) used for completing bacteria detection is fixedly installed at the other end inside the detection case (1);

the detection mechanism (7) comprises a detection machine body (701), cold air equipment is installed inside the detection machine body (701), a cold air outlet (702) used for conveying cold air is formed in the inner wall of the detection machine body (701), an optical lens mechanism (703) used for detecting bacteria is installed inside the detection machine body (701), a loading rotary disc (704) is rotatably installed inside the detection machine body (701), a vessel carrying disc (705) used for placing a culture vessel is rotatably installed inside the loading rotary disc (704) in an annular array mode, and an edge wheel (706) is integrally arranged at the bottom edge of the vessel carrying disc (705);

a reciprocating shaking assembly for rotationally shaking the culture dish is arranged at the edge of the lower part of the carrying rotary table (704), and a rotary driving mechanism for controlling the rotation of the carrying rotary table (704) and the operation of the reciprocating shaking assembly is arranged right below the circle center of the carrying rotary table (704);

a circle center column (707) is fixedly connected to the circle center of the lower surface of the object carrying turntable (704), a quincuncial groove (722) is formed in the tail end of the circle center column (707), the rotary driving mechanism comprises a rotary column (708) rotatably mounted at the bottom of the detection machine body (701), the rotary column (708) is connected with the circle center column (707) in an inserting mode, an inner cylinder (723) is fixedly mounted inside the rotary column (708), a spring telescopic pipe (724) is fixedly mounted at the output end of the inner cylinder (723), a quincuncial insert block (725) movably inserted into the quincuncial groove (722) is mounted at the top end of the spring telescopic pipe (724), a first helical gear (709) and a first gear (711) are fixedly mounted at the upper end and the lower end of the outer surface of the rotary column (708) respectively, a main motor (710) is fixedly mounted at one side of the rotation column (708) inside the detection machine body (701), and a second helical gear meshed with the first helical gear (709) is fixedly mounted at the output end of the main motor (710);

the reciprocating shaking assembly comprises a first side rotating column (712), a second side rotating column (714) and a third side rotating column (718) which are rotatably installed at the bottom of the detection machine body (701), the first side rotating column (712), the second side rotating column (714) and the third side rotating column (718) are located on the same straight line, a second gear (713) meshed with the first gear (711) is fixedly installed at the top end of the first side rotating column (712), the first side rotating column (712) and the second side rotating column (714) are in transmission connection through a belt (715), a rotary disc (716) is fixedly installed at the top end of the second side rotating column (714), an eccentric rod (717) is fixedly connected to the edge of the upper surface of the rotary disc (716), a push rod (719) is fixedly connected to the top end of the third side rotating column (718), an attaching arc-shaped block (721) used for contacting with an edge wheel (706) is fixedly connected to the tail end of the push rod (719), a straight groove (720) is formed in the lower surface of the push rod (719), and the eccentric rod (717) is movably inserted into the straight groove (720);

and a cell vibration separation mechanism (8) for vibrating a culture dish is fixedly arranged under the object carrying turntable (704) and right below the optical lens mechanism (703).

2. The multifunctional food safety bacteria detection system according to claim 1, wherein: cell vibrations separating mechanism (8) include cylinder (801), the output fixed mounting of cylinder (801) has main stage body (802), the top of main stage body (802) is rotated and is installed screw rod (803), just the top one end fixed mounting of main stage body (802) has and is used for making screw rod (803) rotatory motor (804), the top of main stage body (802) is passed through pipe guide bar subassembly straight line and is slided the equipment and has been had supplementary stage body (805), just it is fixed to be provided with the screwed pipe one of screw rod (803) spiro union in supplementary stage body (805) bottom.

3. The multifunctional food safety bacteria detection system according to claim 2, wherein: the top of assisting the stage body (805) is rotated and is installed screw rod two (806), just the top one end fixed mounting of assisting stage body (805) is used for making the rotatory motor two (807) of screw rod two (806), the top of assisting stage body (805) is passed through pipe guide rod subassembly straight line and is slided the equipment and is had balladeur train (808), just fixed being provided with in balladeur train (808) bottom is with the screwed pipe two of screw rod two (806) spiro union, balladeur train (808) top fixed mounting has mini electromagnetic shaker (809) that are used for vibrations household utensils year dish (705).

4. The multifunctional food safety bacteria detection system according to claim 1, wherein: operation transparent case (2) include operation box (201) of being made by transparent plastic board, operation mouth (202) have all been seted up at the outer wall bottom both ends of operation box (201), ware groove (203) that are used for placing the culture dish are seted up to operation box (201) bottom inner wall equidistance, just reminder lamp (204) are all installed to one side of ware groove (203), the one end fixed mounting of operation box (201) one side inner wall has test-tube rack (205) that are used for placing the test tube, just reminder lamp two (206) are installed to test-tube rack (205) bottom outer wall equidistance, oscillation mouth (207) have been seted up to the one end of operation box (201) bottom inner wall, oscillator (4) that are used for vibrating sample and normal saline mixed liquid are seted up to operation box (201) bottom under being located oscillation mouth (207).

5. The multifunctional food safety bacterium detection system according to claim 4, wherein: the other end of the inner wall of one side of the operation box (201) is fixedly provided with an operation prompter (3) for prompting the detection step, and the operation prompter (3) consists of a display (301), a touch screen input assembly (302) and a sequence prompting system (303);

the sequence prompting system (303) is internally provided with a detection step input module (304) for inputting various detection work detection steps and a detection step prompting module (305) for prompting the detection steps through a prompting lamp.

6. The multifunctional food safety bacterium detection system according to claim 5, wherein: a step sequence input module (306) and a corresponding light selection module (307) for prompting the selection of a designated culture dish are arranged in the detection step input module (304);

the detection step prompting module (305) is internally provided with a step sequence display module (308) for displaying the operation steps one by one through the display (301), a prompting lamp linkage module (309) for lighting a prompting lamp near a designated culture dish or test tube, and a next touch screen triggering module (310) for touching the display (301) to perform the next operation.

7. The multifunctional food safety bacteria detection system according to claim 4, wherein: the top one end fixed mounting of control box (201) has normal saline case (5), just normal saline case (5) bottom is connected with drain pipe (501) that extend to control box (201) inside, drain pipe (501) top fixed mounting has control valve (502) that are used for the flow control.

8. The multifunctional food safety bacteria detection system according to claim 4, wherein: the bottom of operation transparent case (2) is assembled and is used for taking away aseptic gas space circulation mechanism (6) of the inside bacterium-containing gas of operation box (201), aseptic gas space circulation mechanism (6) set up air pump body (606) in operation box (201) bottom including the equidistance, operation box (201) bottom one end fixed mounting has driving motor (607), just driving motor (607) output end fixed mounting has working shaft (608) that are used for driving all gas pump bodies (606) together to work, the output of gas pump body (606) has purge tube (605) and is connected with delivery tube (609) through the pipe connection, the purification filter core is all installed to the inside of purge tube (605).

9. The multifunctional food safety bacterium detection system according to claim 8, wherein: a bottom suction pipe (601) is fixedly installed on one side of the bottom of the operation box (201), the top of the bottom suction pipe (601) is connected with a suction branch pipe (602) extending into the operation box (201), the distance between every two adjacent suction branch pipes (602) is gradually increased from the middle to two sides, the bottom of the bottom suction pipe (601) is connected with a curved guide pipe (603) at equal intervals, the tail end of the curved guide pipe (603) is connected and communicated with a purification pipe (605), and the bottommost part of the curved section of the curved guide pipe (603) is connected with an impurity discharging pipe (604) for discharging impurities; operating box (201) one side inner wall top fixed mounting has top trachea (610), just top trachea (610) lateral wall equidistance is connected with air outlet branch pipe (611) that parallel with the horizontal plane, and the end of delivery tube (609) all is connected with top trachea (610) and communicates with each other.

10. A multifunctional food safety bacteria detection method for using the multifunctional food safety bacteria detection system of any one of claims 1-9, which is characterized by comprising the following steps:

s1: the sterile gas space circulation mechanism (6) can enable the interior of the operation box (201) to be in a sterile environment for a long time, an inspector completes preparation work for detection in the operation box (201), physiological saline is injected into a sample through the liquid outlet pipe (501), then the sample and the physiological saline are subjected to oscillation mixing through the oscillator (4), and then the detection sample liquid which is diluted for the first time can be obtained.

S2: extracting primary diluent into two culture dishes according to an operation flow displayed by an operation prompter (3), then placing the two culture dishes into a corresponding dish groove (203) according to a correspondingly lightened prompt lamp I (204), then touching a screen of the operation prompter (3) to display the next operation step, taking down a test tube at a corresponding position in a test tube rack (205) according to the lightened prompt lamp II (206), firstly injecting physiological saline into the test tube, extracting the primary diluent and injecting the primary diluent into the test tube to obtain secondary diluent, touching the screen of the operation prompter (3) again, extracting the secondary diluent in the test tube into the other two culture dishes according to the lightened prompt lamp I (204), and placing the culture dishes into other dish grooves (203) corresponding to the lightened prompt lamp I (204); according to the preset preparation steps, the configuration of each group of liquid is completed, and the liquid is placed into the corresponding position according to the prompt of the corresponding prompt lamp.

S3: according to the detection sequence, the corresponding culture dishes are taken down and are sequentially placed in the corresponding vessel carrying trays (705), then the internal cylinder (723) is started to enable the rotating column (708) and the circle center column (707) to be in a connection state, at the moment, the main motor (710) is started to enable the rotating column (708) to rotate with the carrying turntable (704), and the culture dishes in the first vessel carrying tray (705) are sent to the position right below the optical lens mechanism (703) for detection.

S4: in the process of transporting the first culture dish, the attaching arc-shaped block (721) at the bottom of the carrying rotary disk (704) can simultaneously rotate in a reciprocating manner, the corresponding vessel carrying disk (705) is driven to rotate by virtue of friction between the attaching arc-shaped block (721) and the edge wheel (706), and at the moment, the culture dish in the first vessel carrying disk (705) can correspondingly rotate and shake, so that cells in the culture dish are prevented from sinking and gathering.

S5: in the process of detecting in first culture dish, in order to guarantee that cells in other culture dishes do not sink and gather, the control interior cylinder (723) withdraws the plum blossom inserted block (725) at the moment, and then the main motor (710) continues to work, the rotating column (708) at the moment can not drive the carrying turntable (704) to rotate, but the rotating column (708) can drive the attaching arc-shaped block (721) to rotate in a reciprocating manner, so that the vessel carrying disks (705) at other positions are driven to rotate in a reciprocating manner, and further the culture dishes except the first culture dish can rotate simultaneously.

S6: when the cells are overlapped at the beginning of detection, the mini-vibrator (809) is moved to the position where the overlapped cells exist, when the mini-vibrator (809) is pressed against the lower surface of the corresponding vessel carrying disc (705), the mini-vibrator (809) is started, the liquid in the culture vessel is vibrated by the mini-vibrator (809), and when the cells are not overlapped, the detection work of the cells is started.