CN115590057A - Biological compound preservative and preservation method - Google Patents

Biological compound preservative and preservation method Download PDF

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Publication number
CN115590057A
CN115590057A CN202211366899.XA CN202211366899A CN115590057A CN 115590057 A CN115590057 A CN 115590057A CN 202211366899 A CN202211366899 A CN 202211366899A CN 115590057 A CN115590057 A CN 115590057A
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CN
China
Prior art keywords
fresh
preservative
percent
keeping
chitosan oligosaccharide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211366899.XA
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Chinese (zh)
Inventor
张林夕
周楷轩
魏萍
苏圆
侯慧敏
陈祥福
赵秀山
周绪元
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Luozhuang District Agricultural Technology Extension Center
Linyi Academy of Agricultural Sciences
Shandong Linyi Institute of Modern Agriculture of Zhejiang University
Original Assignee
Luozhuang District Agricultural Technology Extension Center
Linyi Academy of Agricultural Sciences
Shandong Linyi Institute of Modern Agriculture of Zhejiang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Luozhuang District Agricultural Technology Extension Center, Linyi Academy of Agricultural Sciences, Shandong Linyi Institute of Modern Agriculture of Zhejiang University filed Critical Luozhuang District Agricultural Technology Extension Center
Priority to CN202211366899.XA priority Critical patent/CN115590057A/en
Publication of CN115590057A publication Critical patent/CN115590057A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/10Coating with a protective layer; Compositions or apparatus therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
    • A01N3/02Keeping cut flowers fresh chemically
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/14Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/16Coating with a protective layer; Compositions or apparatus therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a biological compound preservative and a preservation method, comprising an agricultural product preservative and a fresh cut flower preservative, wherein the agricultural product preservative comprises a meat preservative and a fruit and vegetable preservative; the meat preservative comprises the effective components of 0.1-1% of chitosan oligosaccharide, 0.01-0.05% of honeysuckle and 1-1.5% of wood vinegar; the effective components of the fruit and vegetable preservative are 0.1 percent of chitosan oligosaccharide, 0.05 percent of honeysuckle and 0.5 percent of wood vinegar; the fresh cut flower preservative comprises the effective components of 0.01 percent of chitosan oligosaccharide, 0.01 percent of honeysuckle, 0.3 to 0.5 percent of wood vinegar and 2 to 4 percent of cane sugar; the invention has the characteristics of safety, no toxicity, no residue and the like, and mainly inhibits the breeding of microorganisms such as bacteria and the like, delays the oxidation speed, prevents the moisture in agricultural products from being reduced, and prolongs the quality guarantee period and the shelf life of the agricultural products.

Description

Biological compound preservative and preservation method
Technical Field
The invention belongs to the technical field of biological products, and particularly relates to a biological compound preservative and a preservation method.
Background
Most of the existing preservatives are chemical preservatives, which not only increase pesticide residues and harm the health of people, but also cause drug resistance of germs. The biological fresh-keeping method is a fresh-keeping method which is relatively safe and environment-friendly and has a larger application prospect and is newly developed in recent years.
Therefore, the biological preservative is compounded by natural plant extracts and animal extracts.
Disclosure of Invention
The invention aims to provide a biological compound preservative and a preservation method.
The technical scheme adopted by the invention for solving the technical problems is as follows: a biological compound antistaling agent comprises an agricultural product antistaling agent and a fresh cut flower antistaling agent, wherein the agricultural product antistaling agent comprises a meat antistaling agent and a fruit and vegetable antistaling agent; the effective components of the meat preservative are 0.1 to 1 percent of chitosan oligosaccharide, 0.01 to 0.05 percent of honeysuckle and 1 to 1.5 percent of wood vinegar; the effective components of the fruit and vegetable preservative are 0.1 percent of chitosan oligosaccharide, 0.05 percent of honeysuckle and 0.5 percent of wood vinegar; the fresh cut flower preservative comprises the effective components of 0.01 percent of chitosan oligosaccharide, 0.01 percent of honeysuckle, 0.3 to 0.5 percent of wood vinegar and 2 to 4 percent of cane sugar.
A biological compound fresh-keeping method comprises fresh-keeping of agricultural products and fresh-keeping of cut flowers, wherein the fresh-keeping of the agricultural products comprises meat fresh-keeping and fruit and vegetable fresh-keeping.
Specifically, the meat preservation method comprises the following steps: taking 1-10 g of chitosan oligosaccharide and 10-15 g of wood vinegar, fully stirring and dissolving in a constant temperature water bath at 60 ℃, cooling, adding 0.5 g of honeysuckle extract, fixing the volume to 1L, preparing a composite solution, and spraying the composite solution on meat products.
Specifically, the fruit and vegetable fresh-keeping method comprises the following steps: taking 1 g of chitosan oligosaccharide and 5 g of wood vinegar, fully stirring and dissolving in a constant-temperature water bath at 60 ℃, cooling, adding 0.5 g of honeysuckle extract, fixing the volume to 1L, preparing a composite solution, and spraying the composite solution on fruit and vegetable products.
Specifically, the fresh-keeping method for the fresh cut flowers comprises the following steps: taking 0.1 g of chitosan oligosaccharide and 4 g of wood vinegar, fully stirring and dissolving in a constant-temperature water bath at 60 ℃, cooling, adding 0.1 g of honeysuckle extract, fixing the volume to 1L to prepare a composite solution, and preparing the composite solution into a bottle insert solution or directly spraying the composite solution on fresh cut flowers.
The invention has the following beneficial effects: the invention has the characteristics of safety, no toxicity, no residue and the like, and mainly inhibits the breeding of microorganisms such as bacteria and the like, delays the oxidation speed, prevents the moisture in agricultural products from being reduced, and prolongs the quality guarantee period and the shelf life of the agricultural products.
Detailed Description
The present invention will now be described in further detail.
Agricultural product preservation:
1) Meat preservation: 0.1-1% of chitosan oligosaccharide plus 0.01-0.05% of honeysuckle plus 1-1.5% of wood vinegar
Taking (1-10) g of chitosan oligosaccharide and (10-15) g of wood vinegar, fully stirring and dissolving in a constant-temperature water bath at 60 ℃, cooling, adding 0.5 g of honeysuckle extract, and fixing the volume to 1L to prepare a composite solution.
2) Fruit and vegetable preservation: 0.1% of chitosan oligosaccharide, 0.05% of honeysuckle and 0.5% of wood vinegar
Taking 1 g of chitosan oligosaccharide and 5 g of wood vinegar, fully stirring and dissolving in a constant-temperature water bath at 60 ℃, cooling, adding 0.5 g of honeysuckle extract, and fixing the volume to 1L to prepare a composite solution.
The method can obviously reduce the rotting rate and the weight loss rate of agricultural products in the storage process and slow down the loss of nutrient components such as vitamin C, reducing sugar and the like. The main characteristics are: (1) the antibacterial range is wide, and the antibacterial agent has an inhibiting or killing effect on most microorganisms causing agricultural product decay; (2) the food has no toxic or side effect, does not generate toxic or harmful substances in the processing and storage processes, and is safe to eat; (3) the addition amount is small, and the bacteriostatic or bactericidal effect can be achieved at low concentration; (4) no peculiar smell is generated, the original flavor of agricultural products is kept, and the requirements of people on green and environmental protection for keeping the agricultural products fresh are met.
Fresh-keeping of the fresh cut flowers:
0.01 percent of chitosan oligosaccharide, 0.01 percent of honeysuckle flower, 0.3 to 0.5 percent of wood vinegar and 2 to 4 percent of cane sugar
Taking 0.1 g of chitosan oligosaccharide and 4 g of wood vinegar, fully stirring and dissolving in a constant temperature water bath at 60 ℃, cooling, adding 0.1 g of honeysuckle extract, and fixing the volume to 1L to prepare a composite solution.
Preservation principle: the organic acid can reduce the pH value of the aqueous solution, inhibit the reproduction of bacteria and the oxidation of certain phenolic substances, reduce the content of free proline and the relative permeability of a plasma membrane, reduce the blockage of flower stems, improve the water absorption capacity and realize the water-needed balance of flower branches. The chitosan oligosaccharide can not only keep the water content in the leaves, but also slow down the decomposition of chlorophyll in the leaves.
The function is as follows: the fresh cut flower preservative is prepared into the vase solution or directly sprayed, the diameter of the fresh cut flower can be increased, the decomposition of chlorophyll on the leaves of the fresh cut flower and the water loss of the fresh flower can be delayed, and the refreshing time can be delayed for 3-7 days.
The preservation mechanism is as follows:
1) Wood vinegar. The wood vinegar is natural organic product extracted from plant, and contains organic compounds such as acids, phenols, ketones and aldehydes as main components, and also contains various trace elements such as K, ca, mg, zn, etc. Wherein the content of acid organic matters is about 80 percent. Organic acids are considered as main active substances for antibiosis and preservation, and can inhibit the propagation of bacteria by lowering pH; on the other hand, the bacterial cell can penetrate through the bacterial cell membrane to enter the cell, so that the cell structure and the charge distribution in the cell of the bacterial are changed, the cell metabolic process is disturbed, and the exudation of intracellular proteins and carbohydrates is caused, so that the bacterial cell dies. The low molecular organic acid is effective against both gram-positive and gram-negative bacteria. The phenolic substance structure contains benzene ring and hydroxyl group, and can directly act on free radical, so that it can inhibit intracellular oxidation reaction in microorganism, inhibit growth of bacterial spore, inhibit Escherichia coli, penicillium and pomegranate dry rot, and is a natural antioxidant with fresh-keeping effect.
2) Honeysuckle flower. The honeysuckle flower is rich in chlorogenic acid, isochlorogenic acid, caffeic acid, luteolin glycoside and other organic acid substances, as well as volatile oil, flavonoids and other components, has various pharmacological activities of antibiosis, antiphlogosis, antivirus and the like, and has stronger inoxidizability. Has certain inhibiting effect on colibacillus, staphylococcus aureus, aspergillus niger, aspergillus flavus, saccharomyces cerevisiae and the like. The active ingredients in the extract mainly reduce the propagation of conidiospore in the germs, destroy the integrity of cell membranes of the germs, influence functional genes of yeast cell walls, reduce the metabolic rate and enzyme activity of organelles, delay the peak occurrence time of the activities of superoxide dismutase (SOD) and Peroxidase (POD), and promote the activity increase of polyphenol oxidase (PPO) and Phenylalanine Ammonia Lyase (PAL), thereby killing the germs, reducing the metabolic activity and finally achieving the fresh-keeping effect.
3) A chitosan oligosaccharide. Chitosan oligosaccharide (polymerization degree of 2-10, deacetylation degree of more than 95%) is a low-molecular polymer obtained by degrading chitosan, and has good barrier property, antibacterial property and water solubility. The protective film with proper thickness and proper air permeability can be formed on the surface of the agricultural product, so that the moisture in the agricultural product is prevented from being dissipated to the outside too fast, the speed of oxygen permeating into the agricultural product is controlled, the respiratory strength of the agricultural product is regulated and controlled, and the nutrient substances of the agricultural product are prevented from being consumed too fast. Meanwhile, the breeding of microorganisms can be inhibited, and the invasion of the microorganisms can be blocked. The disadvantage is that the antioxidant capacity is relatively weak and can be used as an additive component for the freshness-preserving function.
Although the three products have a certain fresh-keeping function when used independently, the bacteriostatic range is limited and the effect is common. After the plant source extract wood vinegar liquid and the honeysuckle liquid are mixed with the animal source product chitosan oligosaccharide, a superposition effect can be generated, the antibacterial range is obviously increased, and the fresh-keeping effect is increased. And the three products have strong water solubility and can be fully dissolved after being compounded. The wood vinegar liquid can enable chitosan oligosaccharide which cannot invade microbial cells to enter the cells by increasing the permeability of microbial cell membranes, and can be combined with genetic materials to generate the effect of blocking or delaying bacterial reproduction.
The present invention is not limited to the above embodiments, and any structural changes made under the teaching of the present invention shall fall within the scope of the present invention, which is similar or similar to the technical solutions of the present invention.
The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims (5)

1. The biological compound preservative is characterized by comprising an agricultural product preservative and a fresh cut flower preservative, wherein the agricultural product preservative comprises a meat preservative and a fruit and vegetable preservative; the meat preservative comprises the effective components of 0.1-1% of chitosan oligosaccharide, 0.01-0.05% of honeysuckle and 1-1.5% of wood vinegar; the effective components of the fruit and vegetable preservative are 0.1 percent of chitosan oligosaccharide, 0.05 percent of honeysuckle and 0.5 percent of wood vinegar; the fresh cut flower preservative comprises the effective components of 0.01 percent of chitosan oligosaccharide, 0.01 percent of honeysuckle, 0.3 to 0.5 percent of wood vinegar and 2 to 4 percent of cane sugar.
2. The biological compound fresh-keeping method is characterized by comprising the steps of fresh-keeping of agricultural products and fresh-keeping of cut flowers, wherein the fresh-keeping of the agricultural products comprises meat fresh-keeping and fruit and vegetable fresh-keeping.
3. The biological compound fresh-keeping method according to claim 2, wherein the fresh-keeping of the meat comprises the following steps: taking 1-10 g of chitosan oligosaccharide and 10-15 g of wood vinegar, fully stirring and dissolving in a constant temperature water bath at 60 ℃, cooling, adding 0.5 g of honeysuckle extract, fixing the volume to 1L, preparing a composite solution, and spraying the composite solution on meat products.
4. The biological compound fresh-keeping method according to claim 1, wherein the fresh-keeping of fruits and vegetables comprises the following steps: taking 1 g of chitosan oligosaccharide and 5 g of wood vinegar, fully stirring and dissolving in a constant-temperature water bath at 60 ℃, cooling, adding 0.5 g of honeysuckle extract, fixing the volume to 1L, preparing a composite solution, and spraying the composite solution on fruit and vegetable products.
5. The biological compound fresh-keeping method according to claim 1, wherein the fresh-keeping of the cut flowers comprises the following steps: taking 0.1 g of chitosan oligosaccharide and 4 g of wood vinegar, fully stirring and dissolving in a constant temperature water bath at 60 ℃, cooling, adding 0.1 g of honeysuckle extract, fixing the volume to 1L to prepare a composite solution, and preparing the composite solution into a bottle-insert liquid or directly spraying the composite solution on fresh cut flowers.
CN202211366899.XA 2022-11-02 2022-11-02 Biological compound preservative and preservation method Pending CN115590057A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211366899.XA CN115590057A (en) 2022-11-02 2022-11-02 Biological compound preservative and preservation method

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Application Number Priority Date Filing Date Title
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050090905A (en) * 2004-03-10 2005-09-14 진민웅 Maturing procedure of bacon
JP2006124365A (en) * 2004-10-28 2006-05-18 Osaka Prefecture Method for producing cut flower freshness-retaining material and freshness-retaining method
CN106070573A (en) * 2016-06-22 2016-11-09 太仓市联新农场专业合作社 A kind of fruit antistaling agent and preparation method thereof
CN106172728A (en) * 2016-08-10 2016-12-07 邓付圆 A kind of fruit and vegetable fresh-keeping agent containing oligochitosan
CN107258771A (en) * 2017-08-01 2017-10-20 芜湖玖荣生物科技有限公司 A kind of cut gerbera flower antistaling agent
CN113100229A (en) * 2021-04-29 2021-07-13 昆明理工大学 Application of chlorogenic acid and honeysuckle extract in delaying blooming of fresh cut flower buds

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050090905A (en) * 2004-03-10 2005-09-14 진민웅 Maturing procedure of bacon
JP2006124365A (en) * 2004-10-28 2006-05-18 Osaka Prefecture Method for producing cut flower freshness-retaining material and freshness-retaining method
CN106070573A (en) * 2016-06-22 2016-11-09 太仓市联新农场专业合作社 A kind of fruit antistaling agent and preparation method thereof
CN106172728A (en) * 2016-08-10 2016-12-07 邓付圆 A kind of fruit and vegetable fresh-keeping agent containing oligochitosan
CN107258771A (en) * 2017-08-01 2017-10-20 芜湖玖荣生物科技有限公司 A kind of cut gerbera flower antistaling agent
CN113100229A (en) * 2021-04-29 2021-07-13 昆明理工大学 Application of chlorogenic acid and honeysuckle extract in delaying blooming of fresh cut flower buds

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