CN115561443B - Activity detection data processing system and method - Google Patents
Activity detection data processing system and method Download PDFInfo
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- CN115561443B CN115561443B CN202211437125.1A CN202211437125A CN115561443B CN 115561443 B CN115561443 B CN 115561443B CN 202211437125 A CN202211437125 A CN 202211437125A CN 115561443 B CN115561443 B CN 115561443B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
Abstract
The invention provides an activity detection data processing system and method, which relate to the technical field of biological activity detection, wherein the processing system comprises a detection acquisition module, a detection setting module and a data analysis module, and the detection acquisition module comprises a biosensor; the detection setting module comprises a freezing setting unit and a thawing setting unit, and is configured with a detection setting strategy, wherein the detection setting strategy comprises; according to the invention, through analyzing activity detection data in different freezing and thawing modes, freezing and thawing parameters of the biological reagent can be adjusted in time, so that the activity of the biological reagent is ensured, and the problems that the activity data analysis of the existing biological reagent is not comprehensive enough, and the freezing and thawing data support is insufficient, so that the activity concentration of the biological reagent is not good enough are solved.
Description
Technical Field
The invention relates to the technical field of biological activity detection, in particular to an activity detection data processing system and method.
Background
Biological activity, in the field of materials, mainly refers to the property of inducing specific biological and chemical reactions at the interface between the material and the biological tissue, which reactions lead to the formation of chemical bonds between the material and the biological tissue. In the biological mineralization process, the capability of chemical bonding between the biological material and the bone of a living body is mainly referred to as an important index for measuring the biological material, and the biological activity of the material in the body can be reflected by the capability of forming apatite on the surface of the material in human body simulated body fluid. Bioactive substances, also called physiologically active substances, i.e. compounds with biological activity, refer to trace or small amount of substances having influence on life phenomena, including polysaccharides, terpenes, sterols, alkaloids, peptides, nucleic acids, proteins, amino acids, glycosides, lipids, waxes, resins, plant pigments, mineral elements, enzymes, vitamins, etc.
In the prior art, since biological reagents are characterized by being particularly sensitive to temperature, very unstable and easy to react with each other, and therefore need to be stored and transported in a freezing way, different reagents have different concentrations of solutions, and the activity of the reagents can change due to the freezing and thawing rates in the freezing and thawing processes, but in the prior art, the freezing and thawing standards with fixed parameters are generally adopted, and different biological reagents cannot be in a better activity concentration, so that a processing method or system based on activity detection data is lacked to solve the existing problems.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide an activity detection data processing system and method, which can adjust the freezing and thawing parameters of a biological reagent in time by analyzing activity detection data in different freezing and thawing modes so as to ensure the activity of the biological reagent, and solve the problems that the activity data analysis of the existing biological reagent is not comprehensive enough, and the freezing and thawing data support is insufficient, so that the activity concentration of the biological reagent is not good enough.
In order to achieve the purpose, the invention is realized by the following technical scheme: the activity detection data processing system comprises a detection acquisition module, a detection setting module and a data analysis module;
the detection acquisition module comprises a biosensor;
the detection setting module comprises a freezing setting unit and a unfreezing setting unit, the detection setting module is configured with a detection setting strategy, and the detection setting strategy comprises: a freezing strategy is set for freezing the biological reagent through the freezing setting unit, and a thawing strategy is set for thawing the biological reagent through the thawing setting unit;
the freezing strategy comprises: setting a plurality of freezing modes, wherein the plurality of freezing modes comprise freezing the biological reagent at different freezing rates; the thawing strategy comprises: setting a plurality of thawing modes, wherein the plurality of thawing strategies comprise thawing the biological reagent at different thawing rates;
the detection and acquisition module is configured with a detection and acquisition strategy, and the detection and acquisition strategy comprises the following steps: acquiring biological activity data in different freezing modes and thawing modes through a biosensor;
the data analysis module is configured with a data analysis policy, the data analysis policy comprising: and analyzing the obtained biological activity data in different freezing modes and thawing modes, and outputting the adjustment results of the freezing mode and the thawing mode of the biological reagent.
Further, the freezing strategy further comprises: setting a first freezing temperature, a second freezing temperature and a third freezing temperature; the first freezing temperature is lower than the second freezing temperature, and the second freezing temperature is lower than the third freezing temperature;
recording the time length from the solution state to the complete crystallization state of the biological reagent at a first freezing temperature, and setting the time length as a first crystallization time length;
recording the time length of the biological reagent from the solution state to the complete crystallization state at a second freezing temperature, and setting the time length as a second crystallization time length;
recording the time length from the solution state to the complete crystallization state of the biological reagent at a third freezing temperature, and setting the time length as a third crystallization time length;
the thawing strategy further comprises: setting a first thawing temperature, a second thawing temperature and a third thawing temperature; the first unfreezing temperature is lower than the second unfreezing temperature, and the second unfreezing temperature is lower than the third unfreezing temperature.
Further, the detection setting policy further includes: unfreezing the three groups of biological reagents with the first crystallization time duration by respectively adopting a first unfreezing mode, a second unfreezing mode and a third unfreezing mode, wherein the first unfreezing mode, the second unfreezing mode and the third unfreezing mode are unfrozen by respectively adopting a first unfreezing temperature, a second unfreezing temperature and a third unfreezing temperature; recording the time length from the completely crystallized state to the solution state of the biological reagent in the first thawing mode, and setting the time length as a first thawing time length; recording the time length from the completely crystallized state to the solution state of the biological reagent in the second thawing mode, and setting the time length as a second thawing time length; recording the time length from the completely crystallized state to the solution state of the biological reagent in a third thawing mode, and setting the time length as a third thawing time length;
thawing the three groups of biological reagents with the second crystallization time length by respectively adopting a fourth thawing mode, a fifth thawing mode and a sixth thawing mode, and thawing the biological reagents with the fourth thawing mode, the fifth thawing mode and the sixth thawing mode by respectively adopting a first thawing temperature, a second thawing temperature and a third thawing temperature; recording the time length from the completely crystallized state to the solution state of the biological reagent in a fourth thawing mode, and setting the time length as a fourth thawing time length; recording the time period from the completely crystallized state to the solution state of the biological reagent in a fifth thawing mode, and setting the time period as a fifth thawing time period; recording the time period from the completely crystallized state to the solution state of the biological reagent in a sixth thawing mode, and setting the time period as a sixth thawing time period;
respectively adopting a seventh thawing mode, an eighth thawing mode and a ninth thawing mode for three groups of biological reagents with the third crystallization time duration, and respectively adopting a first thawing temperature, a second thawing temperature and a third thawing temperature for thawing in the seventh thawing mode, the eighth thawing mode and the ninth thawing mode; recording the time period from the completely crystallized state to the solution state of the biological reagent in the seventh thawing mode, and setting as a seventh thawing time period; recording the time period from the completely crystallized state to the solution state of the biological reagent in the eighth thawing mode, and setting as an eighth thawing time period; the time period from the completely crystallized state to the solution state of the biological agent is recorded in the ninth thawing mode, and is set as the ninth thawing time period.
Further, the detection and acquisition module is configured with a detection and acquisition strategy, which includes: respectively acquiring biological activity electric signals in a first thawing mode, a second thawing mode and a third thawing mode through a biosensor, and respectively setting the biological activity electric signals as a first activity concentration, a second activity concentration and a third activity concentration;
respectively acquiring biological activity electric signals in a fourth thawing mode, a fifth thawing mode and a sixth thawing mode through a biosensor, and respectively setting the biological activity electric signals as a fourth activity concentration, a fifth activity concentration and a sixth activity concentration;
biological activity electric signals in the seventh thawing mode, the eighth thawing mode and the ninth thawing mode are respectively acquired through the biosensor and are respectively set as a seventh activity concentration, an eighth activity concentration and a ninth activity concentration.
Further, the data analysis module includes a freeze analysis unit configured with a freeze analysis strategy, the freeze analysis strategy including: acquiring the maximum values of the first active concentration, the fourth active concentration and the seventh active concentration, and setting the maximum values as a first freezing maximum reference value;
acquiring the maximum values of the second active concentration, the fifth active concentration and the eighth active concentration, and setting the maximum values as a second freezing maximum reference value;
acquiring the maximum values of the third active concentration, the sixth active concentration and the ninth active concentration, and setting the maximum values as a third freezing maximum reference value;
when at least two groups of corresponding freezing temperatures in the first freezing maximum reference value, the second freezing maximum reference value and the third freezing reference value are the same, setting the corresponding freezing temperatures as basic freezing reference temperatures, and outputting freezing setting signals;
and outputting a thawing influence signal when the freezing temperatures corresponding to the first freezing maximum reference value, the second freezing maximum reference value and the third freezing reference value are different from each other.
Further, the data analysis module further includes a thawing analysis unit configured with a thawing analysis policy, the thawing analysis policy including: when a thawing influence signal is received, performing thawing influence analysis;
acquiring the maximum value of the first active concentration, the second active concentration and the third active concentration, and setting the maximum value as a first unfreezing maximum reference value;
acquiring the maximum value of the fourth active concentration, the fifth active concentration and the sixth active concentration, and setting the maximum value as a second unfreezing maximum reference value;
acquiring the maximum value of the seventh active concentration, the eighth active concentration and the ninth active concentration, and setting the maximum value as a third unfreezing maximum reference value;
when at least two groups of corresponding thawing temperatures in the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are the same, setting the corresponding thawing temperatures as basic thawing reference temperatures, and outputting thawing setting signals;
when the thawing temperatures corresponding to the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are different from each other, outputting a preset information signal; the preset information includes: and freezing and thawing the biological reagent according to the preset freezing temperature and thawing temperature.
Further, the freeze setup unit is further configured with a freeze reset strategy comprising: when the base freezing reference temperature is the first freezing temperature, taking the first freezing temperature as a freezing reset temperature;
when the basic freezing reference temperature is the second freezing temperature, setting the freezing temperature corresponding to the maximum value of the first thawing maximum reference value and the third thawing maximum reference value as a freezing reset deviation temperature; solving the freezing reset temperature by the first maximum thawing reference value, the second maximum thawing reference value, the third maximum thawing reference value, the second freezing temperature and the freezing reset deviation temperature through a freezing reset formula; the freeze reset formula is configured to:
(ii) a Wherein, tldc is a frozen reset temperature, tld2 is a second frozen temperature, njd is a first thawing maximum reference value, njd2 is a second thawing maximum reference value, njd is a third thawing maximum reference value, and Tldp is a frozen reset deviation temperature;
and when the base freezing reference temperature is the third freezing temperature, taking the third freezing temperature as the freezing reset temperature.
Further, the thawing setup unit is further configured with a thawing reset policy, the thawing reset policy comprising: when the basic unfreezing reference temperature is the first unfreezing temperature, taking the first unfreezing temperature as the unfreezing reset temperature;
when the base thawing reference temperature is the second thawing temperature,setting the unfreezing temperature corresponding to the maximum value of the first frozen maximum reference value and the third frozen maximum reference value as an unfreezing reset deviation temperature; obtaining the thawing reset temperature by the first freezing maximum reference value, the second freezing maximum reference value, the third freezing maximum reference value, the second thawing temperature and the thawing reset deviation temperature through a thawing reset formula; the thaw reset formula is configured to:
(ii) a Wherein, tjdc is the thawing reset temperature, tjd2 is the second thawing temperature, nld is the first freezing maximum reference value, nld is the second freezing maximum reference value, nld is the third freezing maximum reference value, tjdp is the thawing reset deviation temperature;
and when the basic unfreezing reference temperature is the third unfreezing temperature, taking the third unfreezing temperature as the unfreezing reset temperature.
An activity detection data processing method, comprising the steps of:
step S11, a freezing strategy is set for the freezing of the biological reagent, and the freezing strategy comprises the following steps: setting a plurality of freezing modes, wherein the plurality of freezing modes comprise freezing the biological reagent at different freezing rates;
setting a thawing strategy for thawing the biological reagent; the thawing strategy comprises: setting a plurality of thawing modes, wherein the plurality of thawing strategies comprise thawing the biological reagent at different thawing rates;
s21, acquiring biological activity data in different freezing modes and thawing modes through a biosensor;
and step S31, analyzing the acquired biological activity data in different freezing modes and thawing modes, and outputting the adjustment results of the freezing mode and the thawing mode of the biological reagent.
The invention has the beneficial effects that: the invention sets a freezing strategy through freezing of biological reagents, wherein the freezing strategy comprises the following steps: setting a plurality of freezing modes, wherein the freezing modes comprise freezing the biological reagent at different freezing rates; setting a thawing strategy for thawing the biological reagent; the thawing strategy comprises: setting a plurality of thawing modes, wherein the plurality of thawing strategies comprise thawing the biological reagent at different thawing rates; acquiring biological activity data in different freezing modes and thawing modes through a biosensor; analyzing the obtained biological activity data in different freezing modes and thawing modes, and outputting adjustment results of the freezing mode and the thawing mode of the biological reagent; the method can improve the comprehensiveness of the activity analysis of the biological reagent, so that the freezing and thawing strategies of different biological reagents are adjusted in time, and the activity concentration of the biological reagent is ensured to be in a better range.
Advantages of additional aspects of the invention will be set forth in part in the description of the embodiments which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a functional block diagram of a processing system of the present invention;
FIG. 2 is a partial flow diagram of a processing method of the present invention;
FIG. 3 is a schematic diagram of the frozen reset bias temperature selection of the present invention.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention.
The embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
Example one
Referring to fig. 1, the present invention provides an activity detection data processing system, which includes a detection acquisition module, a detection setting module, and a data analysis module;
the detection acquisition module comprises a biosensor; the biosensor is a device which takes a biological active unit as a sensitive element, combines a chemical conversion element and a physical conversion element and has high selectivity on an analyte. Is an instrument which is sensitive to biological substances and converts the concentration of the biological substances into an electric signal for detection. Is an analysis tool or system composed of immobilized biological sensitive material as recognition element (including enzyme, antibody, antigen, microbe, cell, tissue, nucleic acid, etc.), proper physicochemical transducer (such as oxygen electrode, photosensitive tube, field effect tube, piezoelectric crystal, etc.) and signal amplification device. The biosensor has the functions of a receiver and a converter.
The detection setting module comprises a freezing setting unit and a unfreezing setting unit, the detection setting module is configured with a detection setting strategy, and the detection setting strategy comprises: a freezing strategy is set for freezing the biological reagent through the freezing setting unit, and a thawing strategy is set for thawing the biological reagent through the thawing setting unit; the freezing strategy comprises: setting a plurality of freezing modes, wherein the freezing modes comprise freezing the biological reagent at different freezing rates; the freezing strategy further comprises: setting a first freezing temperature, a second freezing temperature and a third freezing temperature; the first freezing temperature is lower than the second freezing temperature, and the second freezing temperature is lower than the third freezing temperature; recording the time length from the solution state to the complete crystallization state of the biological reagent at a first freezing temperature, and setting the time length as a first crystallization time length; recording the time length from the solution state to the complete crystallization state of the biological reagent at a second freezing temperature, and setting the time length as a second crystallization time length; the time period from the solution state to the fully crystallized state of the bioreagent was recorded at the third freezing temperature and set as the third crystallization time period. The freezing rate and the thawing rate correspond to different freezing temperatures and thawing temperatures.
The thawing strategy comprises: setting a plurality of thawing modes, wherein the plurality of thawing strategies comprise thawing the biological reagent at different thawing rates; the thawing strategy further comprises: setting a first thawing temperature, a second thawing temperature and a third thawing temperature; the first thawing temperature is less than the second thawing temperature, and the second thawing temperature is less than the third thawing temperature. The biological reagent is an active substance, mainly protein products, and is characterized by being particularly sensitive to temperature, very unstable and easy to react with each other. Due to the characteristics, the biological medicament has short life cycle, low-temperature storage, whole cold chain transportation and short storage life, so the use cost is high, the risk cannot be controlled, and the like. Stability of biological agents is a key factor in the industry. Vacuum freeze-drying is a very desirable option. The setting of the freezing mode is realized by adopting the existing vacuum freeze drying technology, and the unfreezing mode is unfrozen slowly under the condition of ice-water bath or ice bath. If the time is critical, most of the frozen reagent can be thawed in a water bath at room temperature or 35 ℃, but careful observation is needed, the reagent is dissolved while shaking, and the reagent needs to be taken out of the water bath after the reagent is partially dissolved, so that the reagent is still close to 0 ℃ in the dissolving process. The thawed reagents may be placed in an ice-water bath or ice bath.
The detection setting policy further includes: unfreezing the three groups of biological reagents with the first crystallization time duration by respectively adopting a first unfreezing mode, a second unfreezing mode and a third unfreezing mode, wherein the first unfreezing mode, the second unfreezing mode and the third unfreezing mode are unfrozen by respectively adopting a first unfreezing temperature, a second unfreezing temperature and a third unfreezing temperature; recording the time length from the complete crystallization state to the solution state of the biological reagent in the first thawing mode, and setting the time length as the first thawing time length; recording the time length from the completely crystallized state to the solution state of the biological reagent in the second thawing mode, and setting the time length as a second thawing time length; recording the time length from the completely crystallized state to the solution state of the biological reagent in a third thawing mode, and setting the time length as a third thawing time length;
thawing the three groups of biological reagents with the second crystallization time length by respectively adopting a fourth thawing mode, a fifth thawing mode and a sixth thawing mode, and thawing the biological reagents with the fourth thawing mode, the fifth thawing mode and the sixth thawing mode by respectively adopting a first thawing temperature, a second thawing temperature and a third thawing temperature; recording the time length from the completely crystallized state to the solution state of the biological reagent in a fourth thawing mode, and setting the time length as a fourth thawing time length; recording the time period from the completely crystallized state to the solution state of the biological reagent in a fifth thawing mode, and setting the time period as a fifth thawing time period; recording the time period from the completely crystallized state to the solution state of the biological reagent in a sixth thawing mode, and setting the time period as a sixth thawing time period;
respectively adopting a seventh thawing mode, an eighth thawing mode and a ninth thawing mode for three groups of biological reagents with the third crystallization time duration, and respectively adopting a first thawing temperature, a second thawing temperature and a third thawing temperature for thawing in the seventh thawing mode, the eighth thawing mode and the ninth thawing mode; recording the time period from the completely crystallized state to the solution state of the biological reagent in the seventh thawing mode, and setting as a seventh thawing time period; recording the time length from the complete crystallization state to the solution state of the biological reagent in an eighth thawing mode, and setting the time length as an eighth thawing time length; the time period from the completely crystallized state to the solution state of the biological agent is recorded in the ninth thawing mode, and is set as the ninth thawing time period.
The detection and acquisition module is configured with a detection and acquisition strategy, and the detection and acquisition strategy comprises: acquiring biological activity data in different freezing modes and thawing modes through a biosensor; the detection and acquisition module is configured with a detection and acquisition strategy, and the detection and acquisition strategy comprises: respectively acquiring biological activity electric signals in a first thawing mode, a second thawing mode and a third thawing mode through a biosensor, and respectively setting the biological activity electric signals as a first activity concentration, a second activity concentration and a third activity concentration;
respectively acquiring biological activity electric signals in a fourth thawing mode, a fifth thawing mode and a sixth thawing mode through a biosensor, and respectively setting the biological activity electric signals as a fourth activity concentration, a fifth activity concentration and a sixth activity concentration;
and respectively acquiring biological activity electric signals in a seventh thawing mode, an eighth thawing mode and a ninth thawing mode through the biosensor, and respectively setting the biological activity electric signals as a seventh activity concentration, an eighth activity concentration and a ninth activity concentration.
In specific implementation, the first freezing temperature, the second freezing temperature and the third freezing temperature are respectively set as follows: -30 degrees celsius, -20 degrees celsius, and-10 degrees celsius; the first thawing temperature, the second thawing temperature and the third thawing temperature are respectively set as: 0 degrees celsius, 18 degrees celsius, and 36 degrees celsius.
Specifically, the method for obtaining the active concentration refers to the following table, which is an active concentration comparison table obtained by different freezing temperatures corresponding to different thawing temperatures;
the data analysis module is configured with a data analysis strategy, and the data analysis strategy comprises the following steps: and analyzing the obtained biological activity data in different freezing modes and thawing modes, and outputting the adjustment results of the freezing mode and the thawing mode of the biological reagent.
The data analysis module includes freezing analysis unit, and freezing analysis unit disposes freezing analysis strategy, and freezing analysis strategy includes: acquiring the maximum values of the first active concentration, the fourth active concentration and the seventh active concentration, and setting the maximum values as a first freezing maximum reference value;
acquiring the maximum values of the second active concentration, the fifth active concentration and the eighth active concentration, and setting the maximum values as a second freezing maximum reference value;
acquiring the maximum value of the third active concentration, the sixth active concentration and the ninth active concentration, and setting the maximum value as a third freezing maximum reference value;
when at least two groups of corresponding freezing temperatures in the first freezing maximum reference value, the second freezing maximum reference value and the third freezing reference value are the same, setting the corresponding freezing temperatures as basic freezing reference temperatures, and outputting freezing setting signals;
and outputting a thawing influence signal when the freezing temperatures corresponding to the first freezing maximum reference value, the second freezing maximum reference value and the third freezing reference value are different from each other.
The data analysis module still includes the analytic unit that unfreezes, and the analytic unit that unfreezes disposes the analytic strategy that unfreezes, and the analytic strategy that unfreezes includes: when a thawing influence signal is received, performing thawing influence analysis;
acquiring the maximum value of the first activity concentration, the second activity concentration and the third activity concentration, and setting the maximum value as a first unfreezing maximum reference value;
acquiring the maximum value of the fourth active concentration, the fifth active concentration and the sixth active concentration, and setting the maximum value as a second unfreezing maximum reference value;
acquiring the maximum value of the seventh active concentration, the eighth active concentration and the ninth active concentration, and setting the maximum value as a third unfreezing maximum reference value;
when at least two groups of corresponding thawing temperatures in the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are the same, setting the corresponding thawing temperatures as basic thawing reference temperatures, and outputting thawing setting signals;
when the thawing temperatures corresponding to the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are different from each other, outputting a preset information signal; the preset information includes: and freezing and thawing the biological reagent according to the preset freezing temperature and thawing temperature.
The freeze setup unit is further configured with a freeze reset strategy comprising: when the base freezing reference temperature is the first freezing temperature, taking the first freezing temperature as a freezing reset temperature;
when the basic freezing reference temperature is the second freezing temperature, setting the freezing temperature corresponding to the maximum value of the first thawing maximum reference value and the third thawing maximum reference value as a freezing reset deviation temperature; obtaining a freezing reset temperature by a freezing reset formula according to the first thawing maximum reference value, the second thawing maximum reference value, the third thawing maximum reference value, the second freezing temperature and the freezing reset deviation temperature; the freeze reset formula is configured as:
(ii) a Wherein Tldc is the frozen resetTemperature, tld is the second freeze temperature, njd is the first thaw maximum reference value, njd is the second thaw maximum reference value, njd is the third thaw maximum reference value, tldp is the freeze reset bias temperature;
when the base freezing reference temperature is the third freezing temperature, taking the third freezing temperature as the freezing reset temperature; because the first freezing temperature is the lowest value of the common freezing temperature, if the first freezing temperature is the corresponding basic freezing reference temperature, the freezing temperature does not need to be adjusted; the third freezing temperature is the highest, and when the third freezing temperature is higher than the third freezing temperature, the corresponding freezing time is too long, and more freezing resources are consumed, so that the freezing temperature does not need to be adjusted. When the basic freezing reference temperature is the second freezing temperature, the maximum value of the first unfreezing maximum reference value and the third unfreezing maximum reference value is selected as the freezing reset deviation temperature, and the purpose is that: the optimal freezing temperature is usually located at one side of the freezing reset deviation temperature, referring to the diagram, there is a certain corresponding relationship between the optimal freezing temperature and the biological activity, and when the temperature difference is the same, the optimal temperature is usually between the freezing reset deviation temperature and the second freezing temperature. Referring specifically to fig. 3, in fig. 3, the abscissa Tld is the freezing temperature and the ordinate Nh is the active concentration, and it can be seen from the figure that the freezing reset bias temperature is the abscissa corresponding to the dashed line on the right side of the peak point, the second freezing temperature is the abscissa corresponding to the dashed line on the left side of the peak point, and the peak point is between the second freezing temperature and the freezing reset bias temperature on the right side.
The thawing setup unit is further configured with a thawing reset strategy comprising: when the basic unfreezing reference temperature is the first unfreezing temperature, taking the first unfreezing temperature as the unfreezing reset temperature;
when the basic thawing reference temperature is the second thawing temperature, setting the thawing temperature corresponding to the maximum value of the first frozen maximum reference value and the third frozen maximum reference value as a thawing reset deviation temperature; solving the thawing reset temperature by a thawing reset formula according to the first freezing maximum reference value, the second freezing maximum reference value, the third freezing maximum reference value, the second thawing temperature and the thawing reset deflection temperature; solution (II)The freeze reset formula is configured as:
(ii) a Wherein, tjdc is the thawing reset temperature, tjd2 is the second thawing temperature, nld is the first freezing maximum reference value, nld is the second freezing maximum reference value, nld is the third freezing maximum reference value, tjdp is the thawing reset deviation temperature;
and when the basic unfreezing reference temperature is the third unfreezing temperature, taking the third unfreezing temperature as the unfreezing reset temperature. Because the first unfreezing temperature is the lowest value of the common unfreezing temperature, if the first unfreezing temperature is the corresponding basic unfreezing reference temperature, the unfreezing temperature does not need to be adjusted; the third thawing temperature is the highest value of the common thawing temperature, and when the third thawing temperature is higher than the third thawing temperature, the corresponding thawing time is too fast, the thawing time is not controlled well, and the problem that the biological activity is influenced by too high temperature easily occurs. When the base thawing reference temperature is the second thawing temperature, the logic for selecting the maximum value of the first frozen maximum reference value and the third frozen maximum reference value as the target for thawing the reset bias temperature may refer to the logic for selecting the frozen reset bias temperature.
Example two
Referring to fig. 2, the present invention further provides an activity detection data processing method, which includes the following steps:
step S11, a freezing strategy is set for freezing the biological reagent, and the freezing strategy comprises the following steps: setting a plurality of freezing modes, wherein the freezing modes comprise freezing the biological reagent at different freezing rates; the freezing strategy also includes the steps of:
step S1111, setting a first freezing temperature, a second freezing temperature, and a third freezing temperature; the first freezing temperature is lower than the second freezing temperature, and the second freezing temperature is lower than the third freezing temperature;
step S1112, recording the time length from the solution state to the complete crystallization state of the biological reagent at the first freezing temperature, and setting the time length as a first crystallization time length; recording the time length from the solution state to the complete crystallization state of the biological reagent at a second freezing temperature, and setting the time length as a second crystallization time length; the time period from the solution state to the fully crystallized state of the bioreagent was recorded at the third freezing temperature and set as the third crystallization time period.
Setting a thawing strategy for thawing the biological reagent; the thawing strategy comprises: setting a plurality of unfreezing modes, wherein the plurality of unfreezing strategies comprise unfreezing the biological reagent at different unfreezing rates; the thawing strategy further comprises the steps of:
step S1121, setting a first thawing temperature, a second thawing temperature, and a third thawing temperature; the first thawing temperature is less than the second thawing temperature, and the second thawing temperature is less than the third thawing temperature.
Step S11 further includes the steps of:
step S1101, thawing three groups of biological reagents with a first crystallization time duration in a first thawing mode, a second thawing mode, and a third thawing mode, respectively, and thawing the biological reagents with the first thawing temperature, the second thawing temperature, and the third thawing mode in the first thawing mode, the second thawing mode, and the third thawing temperature, respectively; recording the time length from the completely crystallized state to the solution state of the biological reagent in the first thawing mode, and setting the time length as a first thawing time length; recording the time length from the completely crystallized state to the solution state of the biological reagent in the second thawing mode, and setting the time length as a second thawing time length; recording the time length from the completely crystallized state to the solution state of the biological reagent in a third thawing mode, and setting the time length as a third thawing time length;
step S1102, respectively thawing the three groups of biological reagents with a second crystallization duration in a fourth thawing mode, a fifth thawing mode and a sixth thawing mode, respectively thawing the biological reagents with the first thawing temperature, the second thawing temperature and the third thawing temperature in the fourth thawing mode, the fifth thawing mode and the sixth thawing mode; recording the time length from the completely crystallized state to the solution state of the biological reagent in a fourth thawing mode, and setting the time length as a fourth thawing time length; recording the time period from the completely crystallized state to the solution state of the biological reagent in a fifth thawing mode, and setting the time period as a fifth thawing time period; recording the time period from the completely crystallized state to the solution state of the biological reagent in a sixth thawing mode, and setting the time period as a sixth thawing time period;
step S1103, respectively adopting a seventh thawing mode, an eighth thawing mode and a ninth thawing mode for the three groups of biological reagents with the third crystallization time duration, and respectively adopting the first thawing temperature, the second thawing temperature and the third thawing temperature for thawing in the seventh thawing mode, the eighth thawing mode and the ninth thawing mode; recording the time period from the completely crystallized state to the solution state of the biological reagent in the seventh thawing mode, and setting as a seventh thawing time period; recording the time period from the completely crystallized state to the solution state of the biological reagent in the eighth thawing mode, and setting as an eighth thawing time period; the time period from the completely crystallized state to the solution state of the biological agent is recorded in the ninth thawing mode, and is set as the ninth thawing time period.
Step S11 further includes the steps of:
step S1131, when the base freezing reference temperature is the first freezing temperature, taking the first freezing temperature as the freezing reset temperature;
step S1132, when the base freezing reference temperature is the second freezing temperature, setting a freezing temperature corresponding to a maximum value of the first thawing maximum reference value and the third thawing maximum reference value as a freezing reset deviation temperature; obtaining a freezing reset temperature by a freezing reset formula according to the first thawing maximum reference value, the second thawing maximum reference value, the third thawing maximum reference value, the second freezing temperature and the freezing reset deviation temperature; the freeze reset formula is configured as:
(ii) a Wherein, tldc is a frozen reset temperature, tld2 is a second frozen temperature, njd is a first thawing maximum reference value, njd2 is a second thawing maximum reference value, njd is a third thawing maximum reference value, and Tldp is a frozen reset deviation temperature;
step S1133, when the base freezing reference temperature is the third freezing temperature, taking the third freezing temperature as the freezing reset temperature;
step S11 further includes the steps of:
step S1141, when the basic thawing reference temperature is a first thawing temperature, taking the first thawing temperature as a thawing reset temperature;
step S1142, when the basic thawing reference temperature is the second thawing temperature, setting the thawing temperature corresponding to the maximum value of the first frozen maximum reference value and the third frozen maximum reference value as a thawing reset deviation temperature; solving the thawing reset temperature by a thawing reset formula according to the first freezing maximum reference value, the second freezing maximum reference value, the third freezing maximum reference value, the second thawing temperature and the thawing reset deflection temperature; the thaw reset formula is configured to:
(ii) a Wherein, tjdc is the thawing reset temperature, tjd2 is the second thawing temperature, nld is the first freezing maximum reference value, nld is the second freezing maximum reference value, nld is the third freezing maximum reference value, tjdp is the thawing reset deviation temperature;
in step S1143, when the basic thawing reference temperature is the third thawing temperature, the third thawing temperature is used as the thawing reset temperature.
S21, acquiring biological activity data in different freezing modes and thawing modes through a biosensor; step S21 further includes the steps of:
step S211, respectively acquiring biological activity electric signals in a first thawing mode, a second thawing mode and a third thawing mode through a biosensor, and respectively setting the biological activity electric signals as a first activity concentration, a second activity concentration and a third activity concentration;
step S212, respectively acquiring biological activity electric signals in a fourth thawing mode, a fifth thawing mode and a sixth thawing mode through a biosensor, and respectively setting the biological activity electric signals as a fourth activity concentration, a fifth activity concentration and a sixth activity concentration;
in step S213, the biological activity electrical signals in the seventh thawing mode, the eighth thawing mode, and the ninth thawing mode are acquired by the biosensor and are set to the seventh activity concentration, the eighth activity concentration, and the ninth activity concentration, respectively.
And step S31, analyzing the acquired biological activity data in different freezing modes and thawing modes, and outputting the adjustment results of the freezing mode and the thawing mode of the biological reagent.
Step S31 further includes the steps of:
step S3111, obtaining a maximum value of the first active concentration, the fourth active concentration, and the seventh active concentration, and setting the maximum value as a first freezing maximum reference value; acquiring the maximum values of the second active concentration, the fifth active concentration and the eighth active concentration, and setting the maximum values as a second freezing maximum reference value; acquiring the maximum value of the third active concentration, the sixth active concentration and the ninth active concentration, and setting the maximum value as a third freezing maximum reference value;
step S3112, when at least two groups of freezing temperatures corresponding to the first freezing maximum reference value, the second freezing maximum reference value, and the third freezing reference value are the same, setting the corresponding freezing temperatures as basic freezing reference temperatures, and outputting freezing setting signals;
step S3113, outputting a thawing-affecting signal when the freezing temperatures corresponding to the first freezing maximum reference value, the second freezing maximum reference value, and the third freezing reference value are different from each other.
Step S31 further includes the steps of:
step S3121, when the thawing influence signal is received, performing thawing influence analysis;
step S3122, obtaining the maximum value of the first activity concentration, the second activity concentration and the third activity concentration, and setting the maximum value as a first unfreezing maximum reference value; acquiring the maximum value of the fourth active concentration, the fifth active concentration and the sixth active concentration, and setting the maximum value as a second unfreezing maximum reference value; acquiring the maximum value of the seventh active concentration, the eighth active concentration and the ninth active concentration, and setting the maximum value as a third unfreezing maximum reference value;
step S3123, when at least two groups of corresponding thawing temperatures in the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are the same, setting the corresponding thawing temperatures as basic thawing reference temperatures, and outputting a thawing setting signal;
step S3124, when the thawing temperatures corresponding to the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are different from each other, outputting a preset information signal; the preset information includes: and freezing and thawing the biological reagent according to the preset freezing temperature and thawing temperature.
The above formulas are all calculated by taking the numerical value of the dimension, the formula is a formula of the latest real situation obtained by collecting a large amount of data and performing software simulation, the preset parameters in the formula are set by the technicians in the field according to the actual situation, if the weight coefficient and the scale coefficient exist, the set size is a specific numerical value obtained by quantizing each parameter, the subsequent comparison is convenient, and as for the size of the weight coefficient and the scale coefficient, the proportional relation between the parameter and the quantized numerical value is not influenced.
In the above embodiments of the present application, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments.
As will be appreciated by one skilled in the art, embodiments of the present invention may be provided as a method, system, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present invention may take the form of a computer program product embodied on one or more computer-usable storage media having computer-usable program code embodied in the medium. The storage medium may be implemented by any type of volatile or nonvolatile storage device or combination thereof, such as a Static Random Access Memory (SRAM), an Electrically Erasable Programmable Read-Only Memory (EEPROM), an Erasable Programmable Read-Only Memory (EPROM), a Programmable Read-Only Memory (PROM), a Read-Only Memory (ROM), a magnetic Memory, a flash Memory, a magnetic disk, or an optical disk. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.
The above-mentioned embodiments are only specific embodiments of the present invention, which are used for illustrating the technical solutions of the present invention and not for limiting the same, and the protection scope of the present invention is not limited thereto, although the present invention is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: any person skilled in the art can modify or easily conceive the technical solutions described in the foregoing embodiments or equivalent substitutes for some technical features within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the embodiments of the present invention, and they should be construed as being included therein. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (5)
1. The activity detection data processing system is characterized by comprising a detection acquisition module, a detection setting module and a data analysis module;
the detection acquisition module comprises a biosensor;
the detection setting module comprises a freezing setting unit and a unfreezing setting unit, the detection setting module is configured with a detection setting strategy, and the detection setting strategy comprises: a freezing strategy is set for the freezing of the biological reagent through a freezing setting unit, and a thawing strategy is set for the thawing of the biological reagent through a thawing setting unit;
the freezing strategy comprises: setting a plurality of freezing modes, wherein the plurality of freezing modes comprise freezing the biological reagent at different freezing rates; the thawing strategy comprises: setting a plurality of thawing modes, wherein the plurality of thawing strategies comprise thawing the biological reagent at different thawing rates;
the detection and acquisition module is configured with a detection and acquisition strategy, and the detection and acquisition strategy comprises the following steps: acquiring biological activity data in different freezing modes and thawing modes through a biosensor;
the data analysis module is configured with a data analysis policy, the data analysis policy comprising: analyzing the obtained biological activity data in different freezing modes and thawing modes, and outputting adjustment results of the freezing mode and the thawing mode of the biological reagent;
the freezing strategy further comprises: setting a first freezing temperature, a second freezing temperature and a third freezing temperature; the first freezing temperature is lower than the second freezing temperature, and the second freezing temperature is lower than the third freezing temperature;
the thawing strategy further comprises: setting a first thawing temperature, a second thawing temperature and a third thawing temperature; the first thawing temperature is lower than the second thawing temperature, and the second thawing temperature is lower than the third thawing temperature;
the detection setting policy further includes: thawing the three groups of biological reagents with the first crystallization duration by adopting a first thawing mode, a second thawing mode and a third thawing mode respectively, and thawing the biological reagents with the first thawing mode, the second thawing mode and the third thawing mode by adopting a first thawing temperature, a second thawing temperature and a third thawing temperature respectively;
thawing the three groups of biological reagents with the second crystallization time length by respectively adopting a fourth thawing mode, a fifth thawing mode and a sixth thawing mode, and thawing the biological reagents with the fourth thawing mode, the fifth thawing mode and the sixth thawing mode by respectively adopting a first thawing temperature, a second thawing temperature and a third thawing temperature;
respectively adopting a seventh thawing mode, an eighth thawing mode and a ninth thawing mode for three groups of biological reagents with the third crystallization time duration, and respectively adopting a first thawing temperature, a second thawing temperature and a third thawing temperature for thawing in the seventh thawing mode, the eighth thawing mode and the ninth thawing mode;
the detection and acquisition module is configured with a detection and acquisition strategy, and the detection and acquisition strategy comprises the following steps: respectively acquiring biological activity electric signals in a first thawing mode, a second thawing mode and a third thawing mode through a biosensor, and respectively setting the biological activity electric signals as a first activity concentration, a second activity concentration and a third activity concentration;
respectively acquiring biological activity electric signals in a fourth thawing mode, a fifth thawing mode and a sixth thawing mode through a biosensor, and respectively setting the biological activity electric signals as a fourth activity concentration, a fifth activity concentration and a sixth activity concentration;
respectively acquiring biological activity electric signals in a seventh thawing mode, an eighth thawing mode and a ninth thawing mode through a biosensor, and respectively setting the biological activity electric signals as a seventh activity concentration, an eighth activity concentration and a ninth activity concentration;
the data analysis module includes a freeze analysis unit configured with a freeze analysis strategy that includes: acquiring the maximum values of the first active concentration, the fourth active concentration and the seventh active concentration, and setting the maximum values as a first freezing maximum reference value;
acquiring the maximum values of the second active concentration, the fifth active concentration and the eighth active concentration, and setting the maximum values as a second freezing maximum reference value;
acquiring the maximum value of the third active concentration, the sixth active concentration and the ninth active concentration, and setting the maximum value as a third freezing maximum reference value;
when at least two groups of corresponding freezing temperatures in the first freezing maximum reference value, the second freezing maximum reference value and the third freezing reference value are the same, setting the corresponding freezing temperatures as basic freezing reference temperatures, and outputting freezing setting signals;
and outputting a thawing influence signal when the freezing temperatures corresponding to the first freezing maximum reference value, the second freezing maximum reference value and the third freezing reference value are different from each other.
2. The activity detection data processing system of claim 1, wherein the data analysis module further comprises a thaw analysis unit configured with a thaw analysis strategy, the thaw analysis strategy comprising: when a thawing influence signal is received, performing thawing influence analysis;
acquiring the maximum value of the first activity concentration, the second activity concentration and the third activity concentration, and setting the maximum value as a first unfreezing maximum reference value;
acquiring the maximum value of the fourth active concentration, the fifth active concentration and the sixth active concentration, and setting the maximum value as a second unfreezing maximum reference value;
acquiring the maximum value of the seventh active concentration, the eighth active concentration and the ninth active concentration, and setting the maximum value as a third unfreezing maximum reference value;
when at least two groups of corresponding thawing temperatures in the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are the same, setting the corresponding thawing temperatures as basic thawing reference temperatures, and outputting thawing setting signals;
when the thawing temperatures corresponding to the first thawing maximum reference value, the second thawing maximum reference value and the third thawing reference value are different from each other, outputting a preset information signal; the preset information includes: and freezing and thawing the biological reagent according to the preset freezing temperature and thawing temperature.
3. The activity detection data processing system of claim 2, wherein the freeze setup unit is further configured with a freeze reset strategy, the freeze reset strategy comprising: when the base freezing reference temperature is the first freezing temperature, taking the first freezing temperature as a freezing reset temperature;
when the basic freezing reference temperature is the second freezing temperature, setting the freezing temperature corresponding to the maximum value of the first thawing maximum reference value and the third thawing maximum reference value as a freezing reset deviation temperature; obtaining a freezing reset temperature by a freezing reset formula according to the first thawing maximum reference value, the second thawing maximum reference value, the third thawing maximum reference value, the second freezing temperature and the freezing reset deviation temperature; the freeze reset formula is configured to:
(ii) a Wherein, tldc is a frozen reset temperature, tld2 is a second frozen temperature, njd is a first thawing maximum reference value, njd2 is a second thawing maximum reference value, njd is a third thawing maximum reference value, and Tldp is a frozen reset deviation temperature;
and when the base freezing reference temperature is the third freezing temperature, taking the third freezing temperature as the freezing reset temperature.
4. The activity detection data processing system of claim 3, wherein the thaw setup unit is further configured with a thaw reset policy, the thaw reset policy comprising: when the basic unfreezing reference temperature is the first unfreezing temperature, taking the first unfreezing temperature as the unfreezing reset temperature;
when the basic thawing reference temperature is the second thawing temperature, setting the thawing temperature corresponding to the maximum value of the first frozen maximum reference value and the third frozen maximum reference value as a thawing reset deviation temperature; solving the thawing reset temperature by a thawing reset formula according to the first freezing maximum reference value, the second freezing maximum reference value, the third freezing maximum reference value, the second thawing temperature and the thawing reset deflection temperature; the thaw reset formula is configured to:
(ii) a Wherein, tjdc is the thawing reset temperature, tjd2 is the second thawing temperature, nld is the first freezing maximum reference value, nld is the second freezing maximum reference value, nld is the third freezing maximum reference value, tjdp is the thawing reset deviation temperature;
and when the basic unfreezing reference temperature is the third unfreezing temperature, taking the third unfreezing temperature as the unfreezing reset temperature.
5. The processing method of the activity detection data processing system according to any one of claims 1 to 4, wherein the processing method comprises the steps of:
step S11, a freezing strategy is set for the freezing of the biological reagent, and the freezing strategy comprises the following steps: setting a plurality of freezing modes, wherein the plurality of freezing modes comprise freezing the biological reagent at different freezing rates;
setting a thawing strategy for thawing the biological reagent; the thawing strategy comprises: setting a plurality of thawing modes, wherein the plurality of thawing strategies comprise thawing the biological reagent at different thawing rates;
s21, acquiring biological activity data in different freezing modes and thawing modes through a biosensor;
and step S31, analyzing the acquired biological activity data in different freezing modes and thawing modes, and outputting the adjustment results of the freezing mode and the thawing mode of the biological reagent.
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