CN115518417A - Defoaming agent composition for chemiluminescent detection reagent, method of use and application - Google Patents
Defoaming agent composition for chemiluminescent detection reagent, method of use and application Download PDFInfo
- Publication number
- CN115518417A CN115518417A CN202211069677.1A CN202211069677A CN115518417A CN 115518417 A CN115518417 A CN 115518417A CN 202211069677 A CN202211069677 A CN 202211069677A CN 115518417 A CN115518417 A CN 115518417A
- Authority
- CN
- China
- Prior art keywords
- defoaming agent
- defoamer
- composition
- reagent
- agent composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002518 antifoaming agent Substances 0.000 title claims abstract description 117
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 103
- 239000000203 mixture Substances 0.000 title claims abstract description 86
- 238000001514 detection method Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000013530 defoamer Substances 0.000 claims abstract description 61
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 46
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 46
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 46
- 239000006260 foam Substances 0.000 claims abstract description 27
- 229920001296 polysiloxane Polymers 0.000 claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims 1
- 238000002038 chemiluminescence detection Methods 0.000 abstract description 37
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 20
- 108090001005 Interleukin-6 Proteins 0.000 description 19
- 229960002685 biotin Drugs 0.000 description 10
- 235000020958 biotin Nutrition 0.000 description 10
- 239000011616 biotin Substances 0.000 description 10
- RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 230000008030 elimination Effects 0.000 description 5
- 238000003379 elimination reaction Methods 0.000 description 5
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 239000004094 surface-active agent Substances 0.000 description 4
- 102000011632 Caseins Human genes 0.000 description 3
- 108010076119 Caseins Proteins 0.000 description 3
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000004088 foaming agent Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 1
- -1 acridine ester Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 229940008099 dimethicone Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000504 luminescence detection Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D19/00—Degasification of liquids
- B01D19/02—Foam dispersion or prevention
- B01D19/04—Foam dispersion or prevention by addition of chemical substances
- B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
- B01D19/0409—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance compounds containing Si-atoms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Dispersion Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Toxicology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present application relates to a defoamer composition for chemiluminescent detection reagents, methods of use and applications. The defoamer composition includes defoamer 204 and dimethylpolysiloxane. The defoaming agent composition for the chemiluminescence detection reagent comprises a defoaming agent 204 and dimethyl polysiloxane, wherein the defoaming agent 204 and the dimethyl polysiloxane in the defoaming agent composition can play a synergistic effect, and when the defoaming agent composition is applied to the chemiluminescence detection reagent, the defoaming agent composition can not only obviously reduce the time for eliminating foam in the chemiluminescence detection reagent, but also does not influence the detection performance of the chemiluminescence detection reagent, and even can improve the detection performance of the reagent to a certain extent. In addition, the use method of the defoaming agent composition is simple and easy, and the defoaming agent composition only needs to be directly added into a solution of a chemiluminescence detection reagent, so that the defoaming agent composition can be better applied to the chemiluminescence detection reagent.
Description
Technical Field
The application relates to the technical field of biological reagents, in particular to a defoaming agent composition for a chemiluminescence detection reagent, and a use method and application thereof.
Background
In a chemiluminescent assay reagent solution for detecting an antigen or antibody, a large amount of foam is generated by shaking the solution during transport and use of the reagent due to the presence of a protective protein for the antigen or antibody that improves the accuracy of detection and a surfactant that prevents aggregation of the antigen or antibody. A large amount of foam generated in a chemical luminescence detection reagent solution can cause that a sample adding needle on a full-automatic chemical luminescence instrument cannot accurately detect the liquid level and an air suction result occurs.
The existing solution is to find that a large amount of foam appears in the reagent after the reagent is received, and the reagent can not be used immediately after the foam on the surface of the solution is observed by naked eyes and disappears. However, this method has the disadvantage that some agents have too much surfactant added, so that the foam disappears too long, sometimes even after several hours. Completely detrimental to the instant use of the customer.
In the prior art, a defoaming agent is added into a chemiluminescence detection reagent solution to reduce the time for eliminating foam in the chemiluminescence detection reagent, but the added defoaming agent also has a great influence on the detection performance of the reagent. It would therefore be desirable to provide an antifoaming agent that is effective in reducing the time to foam elimination in a luminescent detection reagent without adversely affecting the detection performance of the reagent.
Disclosure of Invention
In order to solve the defects of the prior art, the application provides an antifoaming agent composition for a chemiluminescent detection reagent, wherein the antifoaming agent composition comprises an antifoaming agent 204 and dimethyl polysiloxane, and the antifoaming agent composition can obviously reduce the time for eliminating foam in the chemiluminescent detection reagent and does not influence the detection performance of the chemiluminescent detection reagent.
To this end, the present application provides in a first aspect an antifoam composition for use in a chemiluminescent detection reagent comprising antifoam 204 and dimethylpolysiloxane.
The inventor of the application finds that the defoaming agent 204 has no negative influence on the detection performance of a chemiluminescence detection reagent when applied to the reagent, and can reduce the time for eliminating the foam to a certain extent; when the dimethyl polysiloxane is applied to a chemiluminescence detection reagent, the time for eliminating foam in the reagent can be obviously reduced, but the dimethyl polysiloxane has certain negative influence on the detection performance of the reagent; when the defoaming agent 204 and the dimethyl polysiloxane are simultaneously applied to the chemiluminescence detection reagent, the defoaming agent and the dimethyl polysiloxane can play a synergistic role, the time for eliminating foam in the reagent can be reduced to the minimum, the detection performance of the reagent is not negatively affected, the repeatability of the reagent detection can be improved to a certain extent, and unexpected technical effects are achieved.
In some embodiments, the mass ratio of the defoamer 204 to the dimethylpolysiloxane in the defoamer composition is (0.5-1.5): (0.5-1.5).
In the present application, the defoaming agent composition can be made to have better performance by controlling the mass ratio of the defoaming agent 204 to the dimethylpolysiloxane in the defoaming agent composition within the above range.
In some embodiments, the mass ratio of defoamer 204 to dimethylpolysiloxane in the defoamer composition can be, for example, 1.
In some preferred embodiments, the mass ratio of defoamer 204 to dimethylpolysiloxane in the defoamer composition is 1.
By controlling the mass ratio of the defoaming agent 204 to the dimethylpolysiloxane in the defoaming agent composition to 1.
In some embodiments, the defoamer composition further comprises at least one of defoamer SE-15 and silicone defoamer.
In the present application, the performance of the defoaming agent composition can be further improved by further adding at least one of a defoaming agent SE-15 and a silicone defoaming agent to the defoaming agent composition.
In some embodiments, the mass ratio of the defoamer SE-15 to the defoamer 204 is (0.25-0.5): 1.
In some embodiments, the mass ratio of the defoamer SE-15 to the defoamer 204 can be, for example, 1.
In some preferred embodiments, the mass ratio of the antifoaming agent SE-15 to the antifoaming agent 204 is 1.
In the application, the negative influence of the defoaming agent composition on the detection performance of the chemiluminescence detection reagent can be further reduced by further adding the defoaming agent SE-15 with the content into the defoaming agent composition.
In some embodiments, the mass ratio of the silicone defoamer to the foaming agent 204 is (0.25-0.5): 1.
In some embodiments, the mass ratio of the silicone defoamer to the foaming agent 204 can be, for example, 1.
In some preferred embodiments, the mass ratio of the silicone defoamer to the defoamer 204 is 1.
In the present application, the time for the defoaming agent composition to remove the foam in the chemiluminescent detection reagent can be further reduced by further adding the silicone defoaming agent in the above-described amount to the defoaming agent composition.
The defoaming agent composition provided by the application can obviously reduce the elimination time of foams generated by surface active agents such as BSA, casein and the like and Tween 20 and the like on the basis of not influencing other performances of a chemiluminescence detection reagent, and obtains unexpected technical effects.
In the present application, the components of the defoamer composition are all commercially available products, and can be selected by those skilled in the art according to the needs.
In a second aspect, the present application provides a method of using a defoamer composition as described in the first aspect of the present application, the method of use comprising: the defoamer composition is added to a solution of chemiluminescent detection reagent and stirred and mixed.
In the application, the use method of the defoaming agent composition is simple, and the defoaming agent composition is directly added into a solution of a chemiluminescence detection reagent and then stirred and mixed uniformly.
In some embodiments, the defoamer composition is added in an amount such that the final concentration of defoamer 204 and dimethylpolysiloxane are both 0.001 to 0.01wt%.
In some embodiments, the defoamer composition is added in an amount such that the final concentration of defoamer 204 and dimethylpolysiloxane are each 0.001 wt.%, 0.002 wt.%, 0.003 wt.%, 0.004 wt.%, 0.005 wt.%, 0.006 wt.%, 0.007 wt.%, 0.008 wt.%, 0.009 wt.%, or 0.01 wt.%, etc.
In some preferred embodiments, the defoamer composition is added in an amount such that the final concentration of defoamer 204 and dimethylpolysiloxane are both 0.005wt%.
In the present application, the defoaming performance of the defoaming agent composition can be made better by controlling the final concentrations of the defoaming agent 204 and dimethylpolysiloxane in the chemiluminescent detection reagent solution to be within the above ranges. The concentration of the defoaming agent 204 and the dimethyl polysiloxane is too low, so that the defoaming effect cannot be realized; the concentrations of the defoaming agent 204 and the dimethyl polysiloxane are too high, so that the defoaming effect cannot be improved, and the detection performance of the reagent can be greatly negatively influenced.
In some embodiments, the defoamer composition is added in an amount such that the final concentration of defoamer 204 and dimethylpolysiloxane are each 0.001 to 0.01wt%, the final concentration of defoamer SE-15 is 0.001 to 0.005wt%, and the final concentration of silicone defoamer is 0.001 to 0.005wt%.
In a third aspect, there is provided a chemiluminescent detection reagent comprising an antifoam composition as described in the first aspect of the instant application.
In the application, the content of the defoaming agent composition in the chemiluminescence detection reagent enables the final concentration of the defoaming agent 204 and the dimethyl polysiloxane to be 0.001-0.01 wt%; further, the final concentration of the defoaming agent 204 and the dimethyl polysiloxane is 0.001 to 0.01wt%, the final concentration of the defoaming agent SE-15 is 0.001 to 0.005wt%, and the final concentration of the silicone defoaming agent is 0.001 to 0.005wt%.
The chemiluminescence detection reagent is not specifically limited in type, and can be a chemiluminescence detection reagent commonly used for detecting antigens or antibodies.
The beneficial technical effect of this application: the defoaming agent composition for the chemiluminescence detection reagent comprises the defoaming agent 204 and dimethyl polysiloxane, the defoaming agent 204 and the dimethyl polysiloxane in the defoaming agent composition can play a synergistic effect, and when the defoaming agent composition is applied to the chemiluminescence detection reagent, the time for eliminating foam in the chemiluminescence detection reagent can be obviously reduced, and the detection performance of the chemiluminescence detection reagent is not influenced. In addition, the use method of the defoaming agent composition is simple and easy, and the defoaming agent composition only needs to be directly added into a solution of a chemiluminescence detection reagent, so that the defoaming agent composition can be better applied to the chemiluminescence detection reagent.
Detailed Description
In order to make the present application easier to understand, the present application will be further described in detail with reference to the following examples, which are only illustrative and not intended to limit the scope of the present application. The starting materials or components used in the present application may be commercially or conventionally prepared unless otherwise specified.
Dimethicone, silicone defoamer, defoamer SE-15, and defoamer 204 used in the examples described below were obtained from sigma-aldrich.
Example 1
2g of the defoaming agent 204 and 2g of dimethylpolysiloxane were weighed and mixed to obtain a defoaming agent composition 1. The mass ratio of the defoaming agent 204 to the dimethylpolysiloxane in the defoaming agent composition 1 was 1.
Example 2
1g of the defoaming agent 204 and 3g of dimethylpolysiloxane were weighed and mixed to obtain a defoaming agent composition 2. The mass ratio of the defoaming agent 204 to the dimethylpolysiloxane in the defoaming agent composition 2 was 1.
Example 3
3g of the defoaming agent 204 and 1g of dimethylpolysiloxane were weighed and mixed to obtain a defoaming agent composition 3. The mass ratio of the defoaming agent 204 to the dimethylpolysiloxane in the defoaming agent composition 3 was 3.
Example 4
2g of the defoaming agent 204, 2g of dimethylpolysiloxane and 1g of the defoaming agent SE-15 were weighed and mixed to obtain a defoaming agent composition 4.
The mass ratio of the defoaming agent 204 to the dimethylpolysiloxane defoaming agent in the defoaming agent composition 4 was 1, and the mass ratio of the defoaming agent SE-15 to the defoaming agent 204 was 1.
Example 5
2g of the defoaming agent 204, 2g of dimethylpolysiloxane, and 0.5g of the defoaming agent SE-15 were weighed and mixed to obtain a defoaming agent composition 5. The mass ratio of the defoaming agent 204 to the dimethylpolysiloxane defoaming agent in the defoaming agent composition 5 was 1.
Example 6
2g of the defoaming agent 204, 2g of dimethylpolysiloxane, and 1g of the silicone defoaming agent were weighed and mixed to obtain a defoaming agent composition 6. The mass ratio of the defoaming agent 204 to the dimethylpolysiloxane defoaming agent in the defoaming agent composition 6 was 1.
Example 7
2g of the defoaming agent 204, 2g of dimethylpolysiloxane and 0.5g of a silicone defoaming agent were weighed and mixed to obtain a defoaming agent composition 7. The mass ratio of the defoaming agent 204 to the dimethylpolysiloxane defoaming agent in the defoaming agent composition 7 is 1.
Application example 1
This example demonstrates the defoaming performance of the defoamer compositions prepared in examples 1-7, using IL-6 chemiluminescent detection reagent as an example.
The magnetic bead reagent in the IL-6 chemiluminescence detection reagent adopts a streptavidin magnetic bead reagent of JSR company, and the use concentration is 1mg/mL;
preparing a biotin reagent: the IL-6 monoclonal antibody labeled with biotin was diluted with PBS buffer containing 1% BSA, 1% casein protein and 0.05% surfactant Tween 20 at a final antibody concentration of 1. Mu.g/mL, pH = 7.4;
preparation of an acridine ester reagent: the acridinium ester-labeled IL-6 monoclonal antibody was diluted with pH =7.4PBS buffer containing 1% BSA, 1% casein protein and 0.05% Tween 20, at a final antibody concentration of 0.25. Mu.g/mL.
Respectively adding a certain amount of defoaming agent compositions 1-7 into the solutions of the biotin reagent and the acridinium ester reagent to prepare 8 IL-6 chemiluminescence detection reagents 1-8; the final concentrations of the respective components of the defoaming agent compositions 1 to 7 in the solutions of the biotin reagent and the acridinium ester reagent of the chemiluminescent detection reagents 1 to 8 are shown in Table 1.
TABLE 1
The IL-6-containing samples were tested using 8 IL-6 chemiluminescence detection reagents 1-8, each containing the above defoamer compositions 1-7, and the IL-6 chemiluminescence detection reagents without any defoamer were used as control reagents to evaluate the detection performance of the 8 IL-6 chemiluminescence detection reagents 1-8, each containing the above defoamer compositions 1-7, with the specific test results shown in Table 2.
TABLE 2
The biotin reagent and the acridinium ester reagent in the 8 IL-6 chemiluminescent assay reagents 1-7, each containing the above antifoam compositions 1-7, were placed in a vortex oscillator and rapidly shaken for 30 seconds to generate a large amount of foam, then allowed to stand until the foam in the biotin reagent and the acridinium ester reagent was completely eliminated, and the maximum time required for the foam to be eliminated was recorded, with the results shown in Table 3.
TABLE 3
As can be seen from tables 2 and 3, after the defoaming agent compositions 1 to 7 of the present application are added, the longest time required for complete elimination of foam in the chemiluminescent detection reagents 1 to 8 is significantly reduced compared to the control reagent, and the detection performance of the chemiluminescent detection reagents 1 to 8 added with the defoaming agent compositions 1 to 7 is not significantly changed compared to the control reagent, and meanwhile, the detection performance, especially the detection repeatability, of the chemiluminescent detection reagents 1, 4 to 5, and 7 is improved to some extent, thereby obtaining unexpected technical effects.
Comparative example 1
In the comparative example, an IL-6 chemiluminescence detection reagent is taken as an example, and the defoaming performance of the defoaming agent with a single component is verified.
The composition of the IL-6 chemiluminescence detection reagent used was the same as in application example 1.
Respectively adding a certain amount of single-component antifoaming agent into the solutions of biotin reagent and acridinium ester reagent of the IL-6 chemiluminescence detection reagent to prepare 4 IL-6 chemiluminescence detection reagents 9-12; the final concentrations of the single-component antifoaming agents in the solutions of the biotin reagent and the acridinium ester reagent of the chemiluminescent detection reagents 9-12 are shown in Table 4.
TABLE 4
The detection performance of the 4 IL-6 chemiluminescence detection reagents 9-12 added with single-component antifoaming agents was evaluated by using the 4 IL-6 chemiluminescence detection reagents 9-12 to detect IL-6-containing samples, and using IL-6 chemiluminescence detection reagents without any antifoaming agent as control reagents, in the same detection process and conditions as in application example 1, with specific detection results shown in Table 5.
TABLE 5
The biotin reagent and the acridinium ester reagent in the 4 IL-6 chemiluminescence detection reagents 9-12 were placed in a vortex oscillator and rapidly shaken for 30 seconds to generate a large amount of foam, and then the mixture was allowed to stand until the foam in the biotin reagent and the acridinium ester reagent was completely eliminated and the maximum time required for the foam elimination was recorded, and the results are shown in Table 6.
TABLE 6
As can be seen from Table 5, when the defoaming agent 204 was added in an amount of 0.01wt%, the detection performance of the reagent was not significantly changed, and the margin was slightly deteriorated; the blank limit and the repeatability of the reagent added with the dimethyl polysiloxane and the silicone defoamer are obviously deteriorated; the reagent to which the antifoaming agent SE-15 was added was not so poor in blank limit, but was significantly poor in reproducibility. As can be seen from Table 6, the time for the agent added with the single-component defoaming agent to stand to eliminate the foam after being vigorously shaken to generate the foam is significantly shortened compared with the control, but the foam elimination time is still longer compared with the defoaming agent composition provided by the present application.
It should be noted that the above-mentioned embodiments are only for explaining the present application and do not constitute any limitation to the present application. The present application has been described with reference to exemplary embodiments, but the words which have been used herein are words of description and illustration, rather than words of limitation. Modifications may be made to the invention as specified within the scope of the claims and modifications may be made without departing from the scope and spirit of the invention. Although the present application has been described herein with reference to particular means, materials and embodiments, the present application is not intended to be limited to the particulars disclosed herein, but rather the present application extends to all other methods and applications having the same functionality.
Claims (10)
1. An antifoam composition for use in a chemiluminescent detection reagent comprising antifoam agent 204 and dimethylpolysiloxane.
2. The defoamer composition of claim 1, wherein the mass ratio of the defoamer 204 to the dimethylpolysiloxane in the defoamer composition is (0.5 to 1.5): (0.5 to 1.5).
3. The foam composition according to claim 2, wherein the mass ratio of the foam suppressor 204 to the dimethylpolysiloxane in the defoamer composition is 1:1.
4. The defoamer composition of any one of claims 1-3, further comprising at least one of defoamer SE-15 and silicone defoamer.
5. The defoamer composition of claim 4, wherein the mass ratio of the defoamer SE-15 to the defoamer 204 is (0.25 to 0.5): 1.
6. The defoamer composition of claim 4, wherein the mass ratio of the silicone defoamer to the foamer 204 is (0.25 to 0.5): 1.
7. A method of using the defoamer composition of any one of claims 1-6, comprising: the defoamer composition is added to a solution of the chemiluminescent detection reagent and mixed with stirring.
8. The use method according to claim 7, wherein the defoamer composition is added in an amount such that the final concentration of the defoamer 204 and the dimethylpolysiloxane are 0.001 to 0.01wt%.
9. The use method according to claim 6 or 7, wherein the defoamer composition is added in an amount such that the final concentration of the defoamer 204 and the dimethylpolysiloxane is 0.001 to 0.01wt%, the final concentration of the defoamer SE-15 is 0.001 to 0.005wt%, and the final concentration of the silicone defoamer is 0.001 to 0.005wt%.
10. A chemiluminescent detection reagent comprising the antifoam composition of any one of claims 1-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211069677.1A CN115518417A (en) | 2022-09-01 | 2022-09-01 | Defoaming agent composition for chemiluminescent detection reagent, method of use and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211069677.1A CN115518417A (en) | 2022-09-01 | 2022-09-01 | Defoaming agent composition for chemiluminescent detection reagent, method of use and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115518417A true CN115518417A (en) | 2022-12-27 |
Family
ID=84697177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211069677.1A Pending CN115518417A (en) | 2022-09-01 | 2022-09-01 | Defoaming agent composition for chemiluminescent detection reagent, method of use and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115518417A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09107994A (en) * | 1995-10-13 | 1997-04-28 | Toa Denpa Kogyo Kk | Assay of intracellular atp |
| WO2000019201A1 (en) * | 1998-09-25 | 2000-04-06 | Quest Diagnostics Investments Incorporated | Biological assay compositions containing non-interfering, foam-inhibiting and foam-collapsing agents |
| US20040101859A1 (en) * | 2002-11-25 | 2004-05-27 | Cepheid | Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices |
| US7223532B1 (en) * | 1999-11-17 | 2007-05-29 | Haemosys Gmbh | Blood compatible polymer surfaces |
| CN103443614A (en) * | 2010-10-25 | 2013-12-11 | 霍夫曼-拉罗奇有限公司 | Use of signal enhancing compounds in electrochemiluminescence detection |
| CN109072291A (en) * | 2016-04-06 | 2018-12-21 | 生命技术公司 | For synthesizing and detecting composition, method and the kit of nucleic acid |
-
2022
- 2022-09-01 CN CN202211069677.1A patent/CN115518417A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09107994A (en) * | 1995-10-13 | 1997-04-28 | Toa Denpa Kogyo Kk | Assay of intracellular atp |
| WO2000019201A1 (en) * | 1998-09-25 | 2000-04-06 | Quest Diagnostics Investments Incorporated | Biological assay compositions containing non-interfering, foam-inhibiting and foam-collapsing agents |
| US7223532B1 (en) * | 1999-11-17 | 2007-05-29 | Haemosys Gmbh | Blood compatible polymer surfaces |
| US20040101859A1 (en) * | 2002-11-25 | 2004-05-27 | Cepheid | Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices |
| CN103443614A (en) * | 2010-10-25 | 2013-12-11 | 霍夫曼-拉罗奇有限公司 | Use of signal enhancing compounds in electrochemiluminescence detection |
| CN109072291A (en) * | 2016-04-06 | 2018-12-21 | 生命技术公司 | For synthesizing and detecting composition, method and the kit of nucleic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4590169A (en) | Direct particle agglutination immunoassays avoiding false negatives at high antigen concentrations | |
| US5185264A (en) | Diluent buffer and method for diluting an assay component | |
| US4292038A (en) | Formamide-containing latex agglutinating reagent for immunoassay | |
| WO2014021387A1 (en) | Latex agglutination inhibition immunoassay | |
| JPWO2010021399A1 (en) | Cystatin C adsorption inhibitor | |
| CN114509562A (en) | Blocking agent and preservative agent of antibody-coupled microsphere compound and application of blocking agent and preservative agent | |
| CN115518417A (en) | Defoaming agent composition for chemiluminescent detection reagent, method of use and application | |
| AU2005220672A1 (en) | Measurement value lowering inhibitor for immunoassay method and immunoassay method using the same | |
| CN115340910B (en) | Cleaning solution suitable for alkaline phosphatase chemiluminescence system | |
| KR101675412B1 (en) | Immunoassay method and reagent therefor | |
| CN115825427A (en) | Method for reducing non-specific adsorption of magnetic beads, magnetic beads prepared by method and application of magnetic beads | |
| CN112051401B (en) | Pepsinogen's assay kit | |
| CN113740526A (en) | Modified sealing agent and preparation method thereof | |
| CN116008524A (en) | Protein-free universal protection liquid, preparation method thereof and application thereof in mycotoxin quantitative detection kit | |
| CN113484306B (en) | Magnetic particle chemiluminescence cleaning solution and preparation method and application thereof | |
| US6680201B2 (en) | Agent for the removal of turbidity in biological samples | |
| CN114859035A (en) | Antibody diluent, antibody product prepared from same and kit | |
| EP1298438A1 (en) | Insoluble carrier particle nephelometric immunoassay reagent | |
| CN111548416A (en) | Kit and composition for detecting anti-double-stranded DNA antibody and application | |
| CN116718782A (en) | Carboxyl receptor microsphere modified by polyethylene glycol derivative and preparation method and application thereof | |
| CN116819089A (en) | Kit for quantitatively determining concentration of cardiac troponin T | |
| JP2001330616A (en) | Method for manufacturing antigen-binding solid phase | |
| CN116026666B (en) | Plasma sample diluent for D-dimer magnetic particle enzymatic chemiluminescence kit and preparation method thereof | |
| CN114167063B (en) | Latex-enhanced competitive immune turbidimetry detection kit and method and application of tigogenin | |
| CN111638328A (en) | Detection method of porcine reproductive and respiratory syndrome antibody |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |