CN111638328A - Detection method of porcine reproductive and respiratory syndrome antibody - Google Patents
Detection method of porcine reproductive and respiratory syndrome antibody Download PDFInfo
- Publication number
- CN111638328A CN111638328A CN202010498302.1A CN202010498302A CN111638328A CN 111638328 A CN111638328 A CN 111638328A CN 202010498302 A CN202010498302 A CN 202010498302A CN 111638328 A CN111638328 A CN 111638328A
- Authority
- CN
- China
- Prior art keywords
- hole
- sample
- respiratory syndrome
- porcine reproductive
- incubating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 208000005342 Porcine Reproductive and Respiratory Syndrome Diseases 0.000 title claims abstract description 15
- 239000000523 sample Substances 0.000 claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 238000005406 washing Methods 0.000 claims abstract description 13
- 238000004140 cleaning Methods 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 239000013641 positive control Substances 0.000 claims abstract description 10
- 239000013642 negative control Substances 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 239000012470 diluted sample Substances 0.000 claims abstract description 6
- 239000012089 stop solution Substances 0.000 claims abstract description 6
- 238000007865 diluting Methods 0.000 claims abstract description 5
- 238000003113 dilution method Methods 0.000 claims abstract description 5
- 239000003593 chromogenic compound Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 8
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 claims description 6
- 239000003085 diluting agent Substances 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 claims description 6
- 101710141454 Nucleoprotein Proteins 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 239000008363 phosphate buffer Substances 0.000 claims description 4
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 claims description 2
- 230000010355 oscillation Effects 0.000 claims description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 2
- 238000002965 ELISA Methods 0.000 claims 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical group P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims 1
- 239000002953 phosphate buffered saline Substances 0.000 claims 1
- 241000700605 Viruses Species 0.000 abstract description 4
- 238000012549 training Methods 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 2
- 238000002372 labelling Methods 0.000 abstract description 2
- 241000282887 Suidae Species 0.000 description 4
- 238000010009 beating Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 210000003754 fetus Anatomy 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000710788 Lelystad virus Species 0.000 description 1
- 208000005107 Premature Birth Diseases 0.000 description 1
- 206010036590 Premature baby Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 208000021760 high fever Diseases 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Virology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of animal virus antibody detection methods, is used for improving the accuracy of a detection result, and particularly relates to a detection method of a porcine reproductive and respiratory syndrome antibody, which specifically comprises the following steps: diluting the sample by 100 times by adopting a distributed dilution method, adding the diluted sample into a hole of an enzyme-linked reaction plate, simultaneously adding a positive control sample and a negative control sample, attaching a sealing plate film, and incubating for 30 minutes; discarding the liquid in each well, washing each well with a washing solution, then adding the enzyme conjugate to each well, and incubating for 30 minutes; cleaning liquid in each hole clearly, then washing each hole for cleaning, then adding a chromogenic substrate into each hole, and incubating for 15 minutes; adding a stop solution into each hole, uniformly mixing by slight vibration, and then measuring the OD value of each hole at the wavelength of 450mn of an enzyme labeling instrument; the detection method is simple to operate, short in spent time, accurate in detection result, high in detection efficiency and suitable for large-scale popularization and use, and common workers can operate after short-term training.
Description
Technical Field
The invention relates to the technical field of animal virus antibody detection methods, is used for improving the accuracy of detection results, and particularly relates to a detection method of a porcine reproductive and respiratory syndrome antibody.
Background
Porcine reproductive and respiratory syndrome (Porcinereproductive and respiratory syndrome)
syndrome, PRRS) is a highly contagious disease caused by the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) characterized by fever, anorexia, late abortion, premature birth, stillborn fetus, weak fetus and mummy fetus of infected pigs, respiratory disorders of various ages, pigs (especially piglets), which was first discovered in the united states in 1987, and which was first isolated from sick piglets and sows in netherlands Wensvoot et al, then called Lelystad virus, and subsequently isolated from countries and regions in germany, united states, uk, and so on, and which is currently spread throughout north america and europe, globally, and kuo et al, which was first isolated from domestic suspected PRRS infected herds, thus confirming the existence of the disease in 2006, and subsequently, in outbreaks of high PRRS in our country, all ages of susceptible pigs, the disease is characterized by high fever (41), high morbidity (50-100%) and high mortality (20-100%), the disease is spread rapidly, a large number of pigs are killed, and huge economic loss is caused to the pig raising industry, so that a detection technology which has high sensitivity and specificity and can realize on-site rapid screening is urgently needed for prevention and control of epidemic situation occurrence and immune evaluation after vaccination.
The existing detection method generally comprises conventional technologies such as virus separation identification, PCR and the like, but the virus separation has the defects of poor sensitivity, long time consumption and the like; PCR diagnosis is not suitable for popularization and application due to relatively complicated operation and high requirements on experimental sites and experimenters.
Disclosure of Invention
In view of the above, in order to solve the problems of long time consumption, complex operation and high requirements on experimental sites and experimenters in the existing detection method, the application provides the detection method for the porcine reproductive and respiratory syndrome antibody, the detection method is simple to operate, the time spent is short, common workers can operate after short-term training, the detection result is accurate, the detection efficiency is high, and the detection method is suitable for large-scale popularization and use.
The technical scheme provided by the invention is a method for detecting a porcine reproductive and respiratory syndrome antibody, which specifically comprises the following steps:
(1) diluting the sample by 100 times by adopting a distributed dilution method to obtain a diluted sample;
(2) adding the diluted sample and the positive and negative control samples into the holes of the enzyme-linked reaction plate, then attaching a sealing plate film, and incubating for 30 minutes at room temperature;
(3) removing the sealing plate membrane, discarding liquid in each hole, cleaning each hole by using a cleaning solution, removing the cleaning solution in each hole, adding an enzyme conjugate into each hole, and incubating for 30 minutes at room temperature;
(4) cleaning liquid in each hole clearly, then adding washing to each hole for cleaning, removing the washing in each hole, then adding a chromogenic substrate to each hole, and incubating for 15 minutes at room temperature;
(5) adding the stop solution into each hole, mixing the solution evenly by slight vibration, and then measuring the OD value of each hole at the wavelength of 450mn of a microplate reader.
After the OD value of each hole is detected and obtained through the technical scheme of the application, the S/P value is calculated through the following formula, the S/P value obtained through calculation is compared with the OD values of a positive control group and a negative control group, if the S/P value of the sample is larger than or equal to the OD value of the positive control group, the sample is positive, and if the S/P value of the sample is smaller than the OD value of the positive control group, the sample is negative.
Further, the distributed dilution method in the step (1) comprises the following steps: firstly, sequentially adding a sample diluent and sample serum in a volume ratio of 9: 1, and uniformly mixing; and taking the sample diluent and the serum diluted in the first step according to the volume ratio of 9: 1, and uniformly mixing by oscillation.
Further, the volume ratio of the sample added per well in step (2) to the enzyme conjugate added per well in step (3) was 1: 1.
Further, the volume ratio of the sample added to each well in the step (2) to the stop solution added to each well in the step (5) is 2: 1.
Further, the enzyme conjugate is phosphate buffer containing 0.04% by volume of horseradish peroxidase-labeled antibody against porcine reproductive and respiratory syndrome virus N protein.
Further, the pH value of the phosphate buffer solution is 5.5.
Further, the enzyme-linked reaction plate is coated with an antibody of the N protein of the porcine reproductive and respiratory syndrome virus.
Further, the washing solution is tris buffer or phosphate buffer.
Based on the explanation, compared with the prior art, the technical scheme of the application has the advantages that: the technical scheme has the advantages of simple operation, short time consumption, accurate detection result, high detection efficiency and suitability for large-scale popularization and use, and common workers can operate the device after short-term training; and the effect of promoting the prevention and control work of the porcine reproductive and respiratory syndrome virus can be achieved.
Detailed Description
Example 1
A detection method of a porcine reproductive and respiratory syndrome antibody specifically comprises the following steps:
(1) diluting the sample by 100 times, and diluting step by step: firstly adding 90uL of sample diluent, then adding 10uL of sample serum, and uniformly mixing; then 90uL of sample diluent is taken, 10uL of serum diluted in the second step is added, and the mixture is shaken and mixed evenly;
(2) adding 100uL diluted sample and positive and negative control to each well, attaching a sealing plate membrane, and incubating for 30 minutes at room temperature;
(3) removing the sealing plate membrane, discarding the liquid in each hole, beating out and adding 200ul of washing liquid into each hole, washing for 3 times, finally beating out on water-absorbing paper, adding 100ul of enzyme conjugate into each hole, and incubating for 30 minutes at room temperature;
(4) discarding liquid in each hole, removing liquid by beating, adding 200ul of washing liquid into each hole, washing for 3 times, removing liquid by beating on absorbent paper, adding 100ul of chromogenic substrate into each hole, and incubating for 15 minutes at room temperature;
(5) adding 50ul of stop solution into each hole, mixing uniformly by slight vibration, and measuring the OD value of each hole at the wavelength of 450mn of an enzyme-labeling instrument.
Through detecting a control group, the establishment basis of the test is determined as follows: the negative control OD value is less than or equal to 0.3, and the positive control OD value is greater than or equal to 0.4;
the calculation formula is as follows: S/P value (S/P sample OD)450nmNegative control OD450nmAverage value)/(positive control OD450Mean-negative control OD450nmAverage value);
the judgment basis is as follows: the S/P value is more than or equal to 0.40 and is positive; the S/P value is less than 0.40, and the result is negative;
randomly selecting 2 groups of negative control samples, positive control samples and 6 groups of test samples, and detecting by using the detection method provided by the application, wherein the detection results are shown in the following table;
according to the detection results of the table, the data obtained by the detection method are more accurate, and the detection method is simple to operate, high in detection efficiency and suitable for large-scale popularization and use.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (8)
1. A detection method of a porcine reproductive and respiratory syndrome antibody is characterized by comprising the following steps:
(1) diluting the sample by 100 times by adopting a distributed dilution method to obtain a diluted sample;
(2) adding the diluted sample and the positive and negative control samples into the holes of the enzyme-linked reaction plate, then attaching a sealing plate film, and incubating for 30 minutes at room temperature;
(3) removing the sealing plate membrane, discarding liquid in each hole, cleaning each hole by using a cleaning solution, removing the cleaning solution in each hole, adding an enzyme conjugate into each hole, and incubating for 30 minutes at room temperature;
(4) cleaning liquid in each hole clearly, then adding washing to each hole for cleaning, removing the washing in each hole, then adding a chromogenic substrate to each hole, and incubating for 15 minutes at room temperature;
(5) adding the stop solution into each hole, mixing the solution evenly by slight vibration, and then measuring the OD value of each hole at the wavelength of 450mn of a microplate reader.
2. The method for detecting porcine reproductive and respiratory syndrome antibody according to claim 1, wherein the distributed dilution method in step (1) comprises: firstly, sequentially adding a sample diluent and sample serum in a volume ratio of 9: 1, and uniformly mixing; and taking the sample diluent and the serum diluted in the first step according to the volume ratio of 9: 1, and uniformly mixing by oscillation.
3. The method of claim 1, wherein the volume ratio of the sample added to each well in step (2) to the enzyme conjugate added to each well in step (3) is 1: 1.
4. The method for detecting porcine reproductive and respiratory syndrome antibody according to claim 1, wherein the volume ratio of the sample added to each well in step (2) to the stop solution added to each well in step (5) is 2: 1.
5. The method of claim 1, wherein the enzyme conjugate is phosphate buffered saline containing 0.04% by volume of horseradish peroxidase-labeled antibody against the N protein of porcine reproductive and respiratory syndrome virus.
6. The method of claim 5, wherein the pH of the phosphate buffer is 5.5.
7. The method of claim 1, wherein the ELISA plate is coated with an antibody against porcine reproductive and respiratory syndrome virus N protein.
8. The method of claim 1, wherein the washing solution is tris buffer or phosphate buffer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010498302.1A CN111638328A (en) | 2020-06-04 | 2020-06-04 | Detection method of porcine reproductive and respiratory syndrome antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010498302.1A CN111638328A (en) | 2020-06-04 | 2020-06-04 | Detection method of porcine reproductive and respiratory syndrome antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111638328A true CN111638328A (en) | 2020-09-08 |
Family
ID=72331372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010498302.1A Pending CN111638328A (en) | 2020-06-04 | 2020-06-04 | Detection method of porcine reproductive and respiratory syndrome antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111638328A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1525170A (en) * | 2003-09-15 | 2004-09-01 | 南京农业大学 | ELISA reagent box for detecting pig reproduction and respiratory syndrome antibody and usage thereof |
| WO2010062395A2 (en) * | 2008-11-26 | 2010-06-03 | South Dakota State University | Identification of porcine reproductive and respiratory syndrome virus |
| CN102175853A (en) * | 2011-01-07 | 2011-09-07 | 北京大北农科技集团股份有限公司 | ELISA (enzyme linked immunosorbent assay) kit for detecting pig progenitive and respiratory syndrome (PRRS) antibody |
| CN102305859A (en) * | 2011-07-27 | 2012-01-04 | 吉林大学 | Activated toxic antibody kit for detecting porcine reproductive and respiratory syndrome virus |
| CN202614765U (en) * | 2011-12-28 | 2012-12-19 | 滨州职业学院 | Porcine reproductive and respiratory syndrome virus PPA-ELISA (enzyme-linked immuno sorbent assay) antibody detection kit |
| CN103364551A (en) * | 2012-04-01 | 2013-10-23 | 武汉中博生物股份有限公司 | ELISA (enzyme-linked immuno sorbent assay) detection kit for porcine reproductive and respiratory syndrome virus IgM (immunoglobulin m) antibodies as well as preparation method and application of ELISA detection kit |
| CN104655841A (en) * | 2015-02-05 | 2015-05-27 | 深圳市康百得生物科技有限公司 | Kit and detection method for detecting porcine reproductive and respiratory syndrome virus |
| CN106093383A (en) * | 2016-07-26 | 2016-11-09 | 石家庄久正生物科技有限公司 | Porcine reproductive and respiratory syndrome virus antibody ELISA diagnosis reagent kit and preparation method thereof |
-
2020
- 2020-06-04 CN CN202010498302.1A patent/CN111638328A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1525170A (en) * | 2003-09-15 | 2004-09-01 | 南京农业大学 | ELISA reagent box for detecting pig reproduction and respiratory syndrome antibody and usage thereof |
| WO2010062395A2 (en) * | 2008-11-26 | 2010-06-03 | South Dakota State University | Identification of porcine reproductive and respiratory syndrome virus |
| CN102175853A (en) * | 2011-01-07 | 2011-09-07 | 北京大北农科技集团股份有限公司 | ELISA (enzyme linked immunosorbent assay) kit for detecting pig progenitive and respiratory syndrome (PRRS) antibody |
| CN102305859A (en) * | 2011-07-27 | 2012-01-04 | 吉林大学 | Activated toxic antibody kit for detecting porcine reproductive and respiratory syndrome virus |
| CN202614765U (en) * | 2011-12-28 | 2012-12-19 | 滨州职业学院 | Porcine reproductive and respiratory syndrome virus PPA-ELISA (enzyme-linked immuno sorbent assay) antibody detection kit |
| CN103364551A (en) * | 2012-04-01 | 2013-10-23 | 武汉中博生物股份有限公司 | ELISA (enzyme-linked immuno sorbent assay) detection kit for porcine reproductive and respiratory syndrome virus IgM (immunoglobulin m) antibodies as well as preparation method and application of ELISA detection kit |
| CN104655841A (en) * | 2015-02-05 | 2015-05-27 | 深圳市康百得生物科技有限公司 | Kit and detection method for detecting porcine reproductive and respiratory syndrome virus |
| CN106093383A (en) * | 2016-07-26 | 2016-11-09 | 石家庄久正生物科技有限公司 | Porcine reproductive and respiratory syndrome virus antibody ELISA diagnosis reagent kit and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Halbur et al. | Development of a streptavidin-biotin immunoperoxidase procedure for the detection of porcine reproductive and respiratory syndrome virus antigen in porcine lung | |
| Corstjens et al. | Up-converting phosphor technology-based lateral flow assay for detection of Schistosoma circulating anodic antigen in serum | |
| Brock | Diagnosis of bovine viral diarrhea virus infections | |
| CN113009154B (en) | Novel one-step method coronavirus neutralizing antibody magnetic microsphere detection kit and application thereof | |
| Grooms et al. | Screening of neonatal calves for persistent infection with bovine viral diarrhea virus by immunohistochemistry on skin biopsy samples | |
| CN113009153B (en) | New coronavirus neutralizing antibody detection kit based on magnetic particle chemiluminescence and application thereof | |
| Bauermann et al. | Generation of calves persistently infected with HoBi-like pestivirus and comparison of methods for detection of these persistent infections | |
| CN111856027A (en) | New coronavirus antibody detection kit suitable for examination of patients without obvious symptoms | |
| AU738964C (en) | Bovine viral diarrhea virus serum antigen capture immunoassay | |
| WO1989001162A1 (en) | Detection methods | |
| CN111474346B (en) | Porcine epidemic diarrhea virus IgA and IgG antibody detection kit, and preparation method and application thereof | |
| Matas et al. | Evaluation of Meridian ImmunoCard Mycoplasma test for the detection of Mycoplasma pneumoniae-specific IgM in paediatric patients | |
| CN107727854A (en) | A kind of avian infectious bronchitis virus protein chip antibody assay kit and its application | |
| Elschner et al. | Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae | |
| CN117229367A (en) | African swine fever virus epitope polypeptide and ELISA antibody detection kit | |
| CN111638328A (en) | Detection method of porcine reproductive and respiratory syndrome antibody | |
| CN110967480A (en) | Preparation and application of pig epidemic diarrhea virus IgA antibody ELISA kit | |
| CN112014561B (en) | Application of porcine circovirus type 4 specific antigen in preparation of kit for detecting porcine circovirus type 4 antibody and kit | |
| AU2020101172A4 (en) | Kit for detecting foot-and-mouth disease virustype asia1 and detection method | |
| Yu et al. | Recombinant truncated nucleocapsid protein as antigen in a novel immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of severe acute respiratory syndrome coronavirus infection | |
| Seo et al. | A possible complementary tool for diagnosing tuberculosis: a feasibility test of immunohistochemical markers | |
| CN202548133U (en) | Brucella antibody quick test kit | |
| AU2020101144A4 (en) | Kit for detecting anti-foot-and-mouth disease virus 3ab antibody and detection method thereby | |
| CN114705857B (en) | Microporous plate type chemiluminescence detection kit for pig foot-and-mouth disease virus O-type and A-type antibodies and application thereof | |
| CN114740201B (en) | Chemiluminescent detection kit for antibodies gE and gI of porcine pseudorabies virus and application of chemiluminescent detection kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200908 |
|
| RJ01 | Rejection of invention patent application after publication |