CN115508295B - A rapid detection instrument for the activity of feed enzyme preparation - Google Patents
A rapid detection instrument for the activity of feed enzyme preparation Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 145
- 230000000694 effects Effects 0.000 title claims abstract description 35
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000012360 testing method Methods 0.000 claims abstract description 55
- 238000004140 cleaning Methods 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000010438 heat treatment Methods 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 8
- 230000000149 penetrating effect Effects 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 230000000903 blocking effect Effects 0.000 claims description 4
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 238000003556 assay Methods 0.000 claims 3
- 230000002146 bilateral effect Effects 0.000 claims 1
- 239000000654 additive Substances 0.000 abstract description 22
- 230000000996 additive effect Effects 0.000 abstract description 17
- 239000000758 substrate Substances 0.000 abstract description 6
- 238000011002 quantification Methods 0.000 abstract description 4
- 239000006172 buffering agent Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000009434 installation Methods 0.000 description 10
- 230000005540 biological transmission Effects 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 229940079919 digestives enzyme preparation Drugs 0.000 description 7
- 230000010355 oscillation Effects 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- UNTBPXHCXVWYOI-UHFFFAOYSA-O azanium;oxido(dioxo)vanadium Chemical compound [NH4+].[O-][V](=O)=O UNTBPXHCXVWYOI-UHFFFAOYSA-O 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical compound [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 description 2
- 235000011007 phosphoric acid Nutrition 0.000 description 2
- 235000002949 phytic acid Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 108010011619 6-Phytase Proteins 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- FENRSEGZMITUEF-ATTCVCFYSA-E [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] FENRSEGZMITUEF-ATTCVCFYSA-E 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 150000004001 inositols Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 229940083982 sodium phytate Drugs 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Immunology (AREA)
- Pathology (AREA)
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Abstract
Description
技术领域technical field
本发明涉及酶活性测试技术领域，具体涉及一种用于饲料酶制剂活性的快速检测仪器。The invention relates to the technical field of enzyme activity testing, in particular to a rapid detection instrument for the activity of feed enzyme preparations.
背景技术Background technique
饲料酶制剂是一种饲料添加剂，常用的饲料酶制剂为植酸酶，植酸酶是催化植酸及其植酸盐水解为肌醇和磷酸(或磷酸盐)，该添加剂使用于饲料中时能够提高动物对饲料的消化或改善动物体内的代谢效率。Feed enzyme preparation is a feed additive. The commonly used feed enzyme preparation is phytase, which catalyzes the hydrolysis of phytic acid and its phytate into inositol and phosphoric acid (or phosphate). Improve animal digestion of feed or improve metabolic efficiency in animals.
添加剂的活性和浓度对产品质量和使用效果为关键性因素，对添加剂的活性产生影响的原因主要有反应温度、混合溶液的PH值和底物的浓度等。在对添加剂的活性的检测方法常用比色法，在检测过程中根据添加剂的自身特性，添加剂在一定温度和PH的条件下，将底物中植酸钠水解，生成正磷酸和肌醇衍生物，并在酸性溶液中与钒钼酸铵生成黄色，根据钒钼酸铵在酸性环境中的显色确定反应速率。根据添加剂催化的转化速率越快，则添加剂的活力就越高，反之，添加剂催化的转化速率越慢，添加剂的活力就越低。The activity and concentration of additives are the key factors to the product quality and use effect, and the main factors affecting the activity of additives are reaction temperature, pH value of mixed solution and concentration of substrate, etc. The colorimetric method is commonly used in the detection of the activity of additives. During the detection process, according to the characteristics of the additives, the additives hydrolyze the sodium phytate in the substrate under certain temperature and pH conditions to generate orthophosphoric acid and inositol derivatives. , and produce yellow color with ammonium vanadium molybdate in acidic solution, and determine the reaction rate according to the color development of ammonium vanadium molybdate in acidic environment. According to the faster the conversion rate catalyzed by the additive, the higher the activity of the additive is, on the contrary, the slower the conversion rate catalyzed by the additive, the lower the activity of the additive.
现有的对添加剂的活性检测时，通过多组实验进行逐步对比分析，在多组实验进行时，难以确保其余变量的稳定相同，导致检测实验数据的误差大，无法找到精确的饲料酶制剂的最高活性的状态。In the existing detection of the activity of additives, multiple groups of experiments are used for step-by-step comparative analysis. When multiple groups of experiments are carried out, it is difficult to ensure that the remaining variables are stable and the same, resulting in large errors in the detection experimental data, and it is impossible to find accurate feed enzymes. the most active state.
发明内容Contents of the invention
本发明提供一种用于饲料酶制剂活性的快速检测仪器，以解决现有的酶制剂的活性检测误差大的问题。The invention provides a rapid detection instrument for the activity of feed enzyme preparations to solve the problem of large errors in the detection of the activity of the existing enzyme preparations.
本发明的一种用于饲料酶制剂活性的快速检测仪器采用如下技术方案：A kind of rapid detection instrument for the activity of feed enzyme preparation of the present invention adopts following technical scheme:
一种用于饲料酶制剂活性的快速检测仪器，包括检测壳、滑动板、振荡单元和检测装置；A rapid detection instrument for the activity of feed enzyme preparations, comprising a detection shell, a sliding plate, an oscillation unit and a detection device;
检测壳具有检测腔，检测壳上侧壁具有连通检测腔的第一开口，检测壳下侧壁固定连接有控温壳，控温壳内设置有多个单独的控温腔，每组控温腔均与检测腔连通；滑动板设置有多组，每组滑动板均能够在检测腔内上下滑动；每组滑动板上均设置有多组安装块，每组安装块均与滑动板转动连接，每组安装块均用于安装方形试管，在滑动板上下滑动时，设置于一组滑动板上多个试管能够进入同一个控温腔内；振荡单元设置有多组，每组振荡单元均能够驱动同一组滑动板上的多组安装块转动；检测装置包括第一检测板和第二检测板，第一检测板与第二检测板平行设置于多组滑动板的左右两侧，第一检测板与第二检测板能够滑动地设置有检测腔内，使第一检测板与第二检测板距离任意一组滑动板的距离相同，第一检测板上设置有分光光度计发射装置，第二检测板上设置有分光光度计接收装置。The detection shell has a detection cavity, the upper side wall of the detection shell has a first opening connected to the detection cavity, the lower side wall of the detection shell is fixedly connected with a temperature control shell, and a plurality of individual temperature control cavities are arranged in the temperature control shell, each group of temperature control The cavities are all connected to the detection cavity; there are multiple sets of sliding plates, and each set of sliding plates can slide up and down in the detection cavity; each set of sliding plates is equipped with multiple sets of mounting blocks, and each set of mounting blocks is rotatably connected with the sliding plate , each set of mounting blocks is used to install square test tubes. When sliding up and down on the sliding plate, multiple test tubes set on one set of sliding plates can enter the same temperature control chamber; there are multiple sets of oscillation units, and each set of oscillation units It can drive multiple sets of mounting blocks on the same set of sliding plates to rotate; the detection device includes a first detection plate and a second detection plate, and the first detection plate and the second detection plate are arranged in parallel on the left and right sides of multiple sets of sliding plates. The detection plate and the second detection plate are slidably arranged in the detection cavity, so that the distance between the first detection plate and the second detection plate is the same as that of any set of sliding plates. The first detection plate is provided with a spectrophotometer emission device, and the second detection plate The second detection board is provided with a spectrophotometer receiving device.
进一步地，振荡单元包括第一动力源和传动带，每组安装块外周侧壁均设置有多个齿块，第一动力源固定设置于滑动板上，传动带依次缠绕多组安装块和第一动力源的动力输出轴。Further, the oscillating unit includes a first power source and a transmission belt. The outer peripheral side walls of each set of mounting blocks are provided with a plurality of gear blocks. Source PTO shaft.
进一步地，每个控温腔内均设置有多组清洁组件，每组清洁组件均包括两个清洁块，两个清洁块均通过第一弹性件设置于控温腔相对的两侧壁上，且两个清洁块相互靠近的侧壁均为圆弧面，清洁块用于清理方形试管侧壁。Further, multiple groups of cleaning components are arranged in each temperature control chamber, and each group of cleaning components includes two cleaning blocks, and the two cleaning blocks are arranged on the opposite side walls of the temperature control chamber through the first elastic member, And the side walls of the two cleaning blocks close to each other are arc surfaces, and the cleaning blocks are used for cleaning the side walls of the square test tube.
进一步地，控温壳上设置多个进水管和多个排水管，每个进水管连通一个控温腔，每个排水管连通一个控温腔，每个控温腔底部均设置有加热丝，加热丝用于对控温腔内的液体加热，使每个控温腔内的温度均能够单独调整。Further, a plurality of water inlet pipes and a plurality of drain pipes are arranged on the temperature control shell, each water inlet pipe is connected to a temperature control chamber, each drain pipe is connected to a temperature control chamber, and a heating wire is arranged at the bottom of each temperature control chamber, The heating wire is used to heat the liquid in the temperature control chamber, so that the temperature in each temperature control chamber can be adjusted individually.
进一步地，检测壳设置有多组内外贯穿的导向槽，每组导向槽均上下延伸，且每组滑动板均能够沿导向槽上下滑动；检测块外侧壁固定设置有多个第二动力源，每个第二动力源的动力输出轴固定连接一组滑动板，第二动力源用于驱动滑动板沿导向槽上下滑动。Further, the detection shell is provided with multiple sets of guide grooves penetrating inside and outside, each set of guide grooves extends up and down, and each set of sliding plates can slide up and down along the guide grooves; the outer wall of the detection block is fixedly provided with multiple second power sources, The power output shaft of each second power source is fixedly connected with a set of sliding plates, and the second power source is used to drive the sliding plates to slide up and down along the guide groove.
进一步地，安装块为圆台结构，安装块直径大的一端处于直径小的一端上方，多个齿块沿安装块上端侧壁周向均匀分布，安装块上下贯穿滑动板，每组安装块均设置有上下贯穿的方形安装槽。Further, the mounting block is a circular platform structure, the end of the mounting block with a large diameter is above the end with a small diameter, a plurality of tooth blocks are evenly distributed along the circumference of the upper side wall of the mounting block, the mounting block runs through the sliding plate up and down, and each set of mounting blocks is provided with There are square mounting slots that run through up and down.
进一步地，检测腔与控温腔之间设置有保温板，保温板上设置有多个向下贯穿的通孔，每个通孔的直径大于方形试管截面的外接圆直径，且通孔的直径小于安装块上端的直径，在滑动板向下滑动时，安装块下端能够进入控温腔内，且安装块的侧壁挤压两个清洁块相互远离。Further, an insulation board is arranged between the detection chamber and the temperature control chamber, and a plurality of through holes penetrating downwards are arranged on the insulation board, the diameter of each through hole is larger than the diameter of the circumscribed circle of the square test tube section, and the diameter of the through hole is smaller than the diameter of the upper end of the mounting block, when the sliding plate slides downward, the lower end of the mounting block can enter the temperature control cavity, and the side wall of the mounting block pushes the two cleaning blocks away from each other.
进一步地，第一检测板上连接有第一推动杆，第二检测板上连接有第二推动杆。Further, a first push rod is connected to the first detection board, and a second push rod is connected to the second detection board.
进一步地，检测壳上设置有封堵第一开口的盖板。Further, the detection shell is provided with a cover plate to block the first opening.
本发明的有益效果是：本发明的一种用于饲料酶制剂活性的快速检测仪器，包括检测壳、滑动板、振荡单元和检测装置，将多个方形试管安装于固定块上，逐步将添加剂和底物添加至试管内，同时向试管内部添加调整PH值的缓冲剂，安装在同一组滑动板上的多个试管处于同一个控温腔内，且能够对每个控温腔内的温度进行单独调整，使不同组滑动板上的试管能够处于不同温度环境。将影响添加剂活性的三个主要变量进行检测时，控制一个定量，将另外两个变量进行调整，同时启动振荡单元使得添加至试管内的物质混合均匀，防止混合不均匀影响对添加剂活性的检测产生影响，逐个驱动滑动板向上滑动，同时检测一组滑动板上的多个试管，且在每组滑动板向上滑动时，第一检测板与第二检测板均能够相互靠近，使得每组滑动板距离第一检测板与第二检测板的距离相同，进而提高检测精度。The beneficial effect of the present invention is: a kind of rapid detection instrument for the activity of feed enzyme preparation of the present invention, comprises detection shell, sliding plate, oscillation unit and detection device, a plurality of square test tubes are installed on the fixed block, and additive and the substrate are added to the test tube, and at the same time, a buffer to adjust the pH value is added to the inside of the test tube. Multiple test tubes installed on the same set of sliding plates are in the same temperature control chamber, and the temperature in each temperature control chamber can be adjusted. Individual adjustments are made so that the test tubes on different sets of sliding plates can be in different temperature environments. When detecting the three main variables that affect the activity of the additive, control one quantitatively, adjust the other two variables, and start the oscillation unit at the same time to make the substances added to the test tube mix evenly, so as to prevent uneven mixing from affecting the detection of the additive activity. Influence, drive the sliding plates to slide upward one by one, and detect multiple test tubes on a set of sliding plates at the same time, and when each set of sliding plates slides upwards, the first detection plate and the second detection plate can be close to each other, so that each set of sliding plates The distance from the first detection board is the same as that of the second detection board, thereby improving the detection accuracy.
附图说明Description of drawings
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动性的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention. For those skilled in the art, other drawings can also be obtained according to these drawings without any creative effort.
图1为本发明实施例的一种用于饲料酶制剂活性的快速检测仪器的结构示意图；Fig. 1 is the structural representation of a kind of rapid detection instrument for the activity of feed enzyme preparation of the embodiment of the present invention;
图2为本发明实施例的一种用于饲料酶制剂活性的快速检测仪器中隐去盖板后的俯视图；Fig. 2 is a top view after the cover plate is hidden in a kind of rapid detection instrument for the activity of feed enzyme preparation according to the embodiment of the present invention;
图3为图2中A-A方向的剖视图；Fig. 3 is the sectional view of A-A direction in Fig. 2;
图4为图3中B处的局部放大图；Fig. 4 is a partial enlarged view of place B in Fig. 3;
图5为本发明实施例的一种用于饲料酶制剂活性的快速检测仪器中隐去检测壳和控温壳等结构的结构示意图；Fig. 5 is a structural schematic diagram of a detection shell and a temperature control shell hidden in a rapid detection instrument for the activity of feed enzyme preparations according to an embodiment of the present invention;
图6为本发明实施例的一种用于饲料酶制剂活性的快速检测仪器中一组滑动板、电热丝等结构的结构示意图；Fig. 6 is a structural schematic diagram of a group of sliding plates, heating wires and other structures in a rapid detection instrument for the activity of feed enzyme preparations according to an embodiment of the present invention;
图7为本发明实施例的一种用于饲料酶制剂活性的快速检测仪器中安装块的结构示意图。Fig. 7 is a schematic structural diagram of a mounting block in a rapid detection instrument for the activity of feed enzyme preparations according to an embodiment of the present invention.
图中：110、检测壳；111、检测腔；113、盖板；114、导向槽；120、控温壳；121、控温腔；130、保温板；140、加热丝；150、进水管；170、显示器；210、滑动板；220、第二气缸；230、安装块；231、安装槽；232、齿块；310、传动带；320、第一电机；321、传动齿轮；410、清洁块；420、第一弹簧；510、第一检测板；520、第二检测板；530、第一推动杆；540、第二推动杆；600、试管。In the figure: 110, detection shell; 111, detection cavity; 113, cover plate; 114, guide groove; 120, temperature control shell; 121, temperature control cavity; 130, heat preservation board; 140, heating wire; 150, water inlet pipe; 170, display; 210, sliding plate; 220, second cylinder; 230, installation block; 231, installation slot; 232, gear block; 310, transmission belt; 320, first motor; 321, transmission gear; 410, cleaning block; 420, the first spring; 510, the first detection plate; 520, the second detection plate; 530, the first push rod; 540, the second push rod; 600, the test tube.
具体实施方式Detailed ways
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
本文中为组件所编序号本身，例如“第一”、“第二”等，仅用于区分所描述的对象，不具有任何顺序或技术含义。而本申请所说“连接”、“联接”，如无特别说明，均包括直接和间接连接(联接)。在本发明的描述中，需要理解的是，术语“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”、“顺时针”、“逆时针”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。The serial numbers assigned to the components in this document, such as "first", "second", etc., are only used to distinguish the described objects and do not have any sequence or technical meaning. The "connection" and "connection" mentioned in this application all include direct and indirect connection (connection) unless otherwise specified. In describing the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", The orientation or positional relationship indicated by "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description , rather than indicating or implying that the device or element referred to must have a particular orientation, be constructed and operate in a particular orientation, and thus should not be construed as limiting the invention.
在本发明中，除非另有明确的规定和限定，第一特征在第二特征“上”或“下”可以是第一和第二特征直接接触，或第一和第二特征通过中间媒介间接接触。而且，第一特征在第二特征“之上”、“上方”和“上面”可以是第一特征在第二特征正上方或斜上方，或仅仅表示第一特征水平高度高于第二特征。第一特征在第二特征“之下”、“下方”和“下面”可以是第一特征在第二特征正下方或斜下方，或仅仅表示第一特征水平高度小于第二特征。In the present invention, unless otherwise clearly specified and limited, the first feature may be in direct contact with the first feature or the first and second feature may be in direct contact with the second feature through an intermediary. touch. Moreover, "above", "above" and "above" the first feature on the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. "Below", "beneath" and "beneath" the first feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is less horizontally than the second feature.
本发明的一种用于饲料酶制剂活性的快速检测仪器的实施例，如图1至图7所示，包括检测壳110、滑动板210、振荡单元和检测装置。An embodiment of a rapid detection instrument for feed enzyme activity of the present invention, as shown in Figures 1 to 7, includes a detection shell 110, a sliding plate 210, an oscillation unit and a detection device.
检测壳110具有检测腔111，检测壳110呈长方体结构，检测壳110水平设置，且检测壳110左右延伸，检测壳110内部中空的为检测腔111，检测壳110上端面具有连通检测腔111的第一开口，检测壳110上设置有封堵第一开口的盖板113。检测壳110下端面中部固定连接有控温壳120，控温壳120呈矩形状，控温壳120的前后宽度与检测壳110的前后宽度一致，控温壳120的水平截面大小与盖板113的截面大小一致。控温壳120内设置有至少三个单独的控温腔121，三个控温腔121在控温壳120内左右均匀分布，每组控温腔121均与检测腔111连通。具体地，控温腔121与检测腔111之间固定设置有保温板130，每个控温腔121上方的保温板130均设置有多个上下贯穿的通孔，通孔用于连通检测腔111与控温腔121，且连通同一个控温腔121与检测腔111的多个通孔在保温板130上前后均匀分布。检测壳110上设置有至少三组前后贯穿的导向槽114，每组导向槽114均竖直设置，三组导向槽114均沿第一开口的左右长度均匀分布。The detection shell 110 has a detection cavity 111, the detection shell 110 is a cuboid structure, the detection shell 110 is arranged horizontally, and the detection shell 110 extends left and right, the hollow inside the detection shell 110 is the detection cavity 111, and the upper end surface of the detection shell 110 has a connection with the detection cavity 111. For the first opening, the detection case 110 is provided with a cover plate 113 for blocking the first opening. The temperature control shell 120 is fixedly connected to the middle part of the lower end surface of the detection shell 110. The temperature control shell 120 is rectangular. The front and rear width of the temperature control shell 120 is consistent with the front and rear width of the detection shell 110. The cross-sectional size is the same. The temperature control housing 120 is provided with at least three separate temperature control chambers 121 , and the three temperature control chambers 121 are evenly distributed left and right in the temperature control housing 120 , and each group of temperature control chambers 121 is connected with the detection chamber 111 . Specifically, a heat preservation board 130 is fixedly arranged between the temperature control chamber 121 and the detection chamber 111. The heat preservation board 130 above each temperature control chamber 121 is provided with a plurality of through holes penetrating up and down, and the through holes are used to communicate with the detection chamber 111. A plurality of through holes connected with the temperature control chamber 121 and connected with the same temperature control chamber 121 and the detection chamber 111 are evenly distributed front and rear on the heat preservation board 130 . The detection shell 110 is provided with at least three sets of guide grooves 114 penetrating front and back, each set of guide grooves 114 is vertically arranged, and the three sets of guide grooves 114 are evenly distributed along the left and right lengths of the first opening.
滑动板210至少设置有三组，滑动板210水平设置，滑动板210前后延伸，每组滑动板210滑动设置于一组导向槽114内，使滑动板210中部处于检测腔111内。检测壳110的后侧壁至少设置有三组第二动力源，第二动力源用于驱动滑动板210沿导向槽114上下滑动。具体地，第二动力源为第二气缸220，每个第二气缸220固定设置于检测壳110后侧壁，每个第二气缸220的动力输出轴固定连接一组滑动板210的后端，在第二气缸220启动时，第二气缸220推动滑动板210沿导向槽114上下滑动。每组滑动板210上均设置有多个上下贯穿的安装孔，多个安装孔沿滑动板210的长度方向均匀分布，每个安装孔内均转动连接有一组安装块230，每组安装块230上均设置有上下贯穿的安装槽231，每个安装槽231均为方槽，方形试管600能够插入安装槽231内，安装块230用于对方形试管600进行固定。每组安装块230均为圆台结构，每组安装块230均竖直设置，每组安装块230的中部与安装槽231侧壁转动连接，使安装块230的上端处于滑动板210的上方，安装块230的下端处于滑动板210的下方。每组安装块230上端的直径大于下端的直径，安装槽231同轴贯穿安装块230，保温板130上通孔的直径大于方形试管600水平截面的外接圆的直径，且通孔的直径大于安装块230最下端的直径，通孔的直径小于安装块230最上端的直径。There are at least three sets of sliding plates 210, the sliding plates 210 are arranged horizontally, and the sliding plates 210 extend forward and backward. At least three sets of second power sources are provided on the rear side wall of the detection case 110 , and the second power sources are used to drive the sliding plate 210 to slide up and down along the guide groove 114 . Specifically, the second power source is a second cylinder 220, each second cylinder 220 is fixedly arranged on the rear side wall of the detection housing 110, and the power output shaft of each second cylinder 220 is fixedly connected to the rear end of a group of sliding plates 210, When the second cylinder 220 is activated, the second cylinder 220 pushes the sliding plate 210 to slide up and down along the guide groove 114 . Each group of sliding plates 210 is provided with a plurality of mounting holes penetrating up and down, and the plurality of mounting holes are evenly distributed along the length direction of the sliding plate 210. A group of mounting blocks 230 are rotatably connected in each mounting hole, and each group of mounting blocks 230 There are mounting grooves 231 that penetrate up and down, each mounting groove 231 is a square groove, the square test tube 600 can be inserted into the mounting groove 231, and the mounting block 230 is used to fix the square test tube 600. Each group of mounting blocks 230 is a round platform structure, and each group of mounting blocks 230 is all vertically arranged, and the middle part of each group of mounting blocks 230 is connected with the side wall of the mounting groove 231 to rotate, so that the upper end of the mounting block 230 is above the sliding plate 210, and the installation The lower end of the block 230 is under the slide plate 210 . The diameter of the upper end of each group of mounting blocks 230 is greater than the diameter of the lower end, and the mounting groove 231 runs through the mounting block 230 coaxially. The diameter of the lowermost end of the block 230 and the diameter of the through hole are smaller than the diameter of the uppermost end of the mounting block 230 .
振荡单元至少设置有三组，每组振荡单元均能够驱动同一组滑动板210上的多组安装块230转动。具体地，每组振荡单元均包括第一动力源和传动带310，每组安装块230的上端周向均匀设置有多个齿块232，第一动力源为第一电机320，第一电机320的动力输出轴上固定设置有传动齿轮321。每个第一电机320均固定设置在一组滑动板210的前端，传动齿轮321与安装块230处于同一水高度，传动带310依次缠绕多组安装块230和一个传动齿轮321，在第一电机320启动时，同一组滑动板210上的多组安装块230同步转动，安装块230带动方形试管600同步转动，使得加入试管600内的试剂混合均匀。There are at least three sets of oscillating units, and each set of oscillating units can drive multiple sets of mounting blocks 230 on the same set of sliding plates 210 to rotate. Specifically, each group of oscillating units includes a first power source and a transmission belt 310, and a plurality of tooth blocks 232 are evenly arranged on the upper end of each group of mounting blocks 230 in the circumferential direction. The first power source is the first motor 320, and the first motor 320 A transmission gear 321 is fixedly arranged on the power output shaft. Each first motor 320 is fixedly arranged on the front end of a group of sliding plates 210, and the transmission gear 321 is at the same water level as the installation block 230. When starting, multiple sets of mounting blocks 230 on the same set of sliding plates 210 rotate synchronously, and the mounting blocks 230 drive the square test tubes 600 to rotate synchronously, so that the reagents added into the test tubes 600 are mixed evenly.
在本实施例中，控温壳120上至少设置有三个进水管150和三个排水管，每个进水管150均设置于控温壳120的前侧壁，每个排水管均设置于控温壳120的后侧壁，每个进水管150连通一个控温腔121，每个进水管150均能够向控温腔121内的注水，每个排水管连通一个控温腔121，每个排水管均能够将控温腔121内的水排放。每个控温腔121内均设置有加热丝140，加热丝140能够对控温腔121内的液体温度进行加热，且每个加热丝140均能够单独控制，使每个控温腔121内的温度均能够单独调整。In this embodiment, at least three water inlet pipes 150 and three drain pipes are arranged on the temperature control shell 120, each water inlet pipe 150 is set on the front side wall of the temperature control shell 120, and each drain pipe is set on the temperature control shell 120. On the rear side wall of the shell 120, each water inlet pipe 150 communicates with a temperature control chamber 121, each water inlet pipe 150 can inject water into the temperature control chamber 121, each drain pipe communicates with a temperature control chamber 121, and each drain pipe Both can discharge the water in the temperature control chamber 121 . Each temperature control chamber 121 is provided with a heating wire 140, the heating wire 140 can heat the temperature of the liquid in the temperature control chamber 121, and each heating wire 140 can be controlled separately, so that the liquid temperature in each temperature control chamber 121 The temperature can be adjusted individually.
在本实施例中，每个控温腔121内均设置有多组清洁组件，多组清洁组件与同一组滑动板210上的多组安装块230一一对应设置，每组清洁组件用于清理附着于一个试管600侧壁的水渍。具体地，每组清洁组件均包括两个清洁块410，两个清洁块410均为半球块结构，且两个清洁块410均通过第一弹性件设置于控温腔121相对的左右两侧壁上，两个清洁块410均能够在控温腔121内左右滑动。具体地，第一弹性件为第一弹簧420，第一弹簧420处于原长时，两个清洁块410互相抵接，且两个清洁块410相互靠近的两侧壁为圆弧面，在滑动板210滑动至导向槽114的下端时，安装块230的下端进入控温腔121，安装块230的侧壁挤压两个清洁块410相互远离，且两个清洁块410在第一弹簧420的作用下抵接安装块230的侧壁，在试管600转动时，两个清洁块410沿安装块230的侧壁周向滑动。In this embodiment, each temperature control cavity 121 is provided with multiple sets of cleaning components, which are arranged in one-to-one correspondence with multiple sets of mounting blocks 230 on the same set of sliding plates 210, and each set of cleaning components is used for cleaning A water stain attached to the side wall of a test tube 600. Specifically, each group of cleaning components includes two cleaning blocks 410, both of which are hemispherical block structures, and the two cleaning blocks 410 are arranged on the left and right side walls of the temperature control chamber 121 through the first elastic member. Above, the two cleaning blocks 410 can slide left and right in the temperature control cavity 121 . Specifically, the first elastic member is the first spring 420. When the first spring 420 is at its original length, the two cleaning blocks 410 abut against each other, and the two side walls of the two cleaning blocks 410 that are close to each other are arc surfaces. When the plate 210 slides to the lower end of the guide groove 114, the lower end of the mounting block 230 enters the temperature control cavity 121, and the side wall of the mounting block 230 pushes the two cleaning blocks 410 away from each other, and the two cleaning blocks 410 are in the position of the first spring 420. When the test tube 600 rotates, the two cleaning blocks 410 slide circumferentially along the side wall of the installation block 230 .
检测装置包括第一检测板510和第二检测板520，第一检测板510与第二检测板520均滑动设置于检测腔111内，第一检测板510设置于检测腔111的右侧，第二检测板520设置于检测腔111的左侧，第一检测板510与第二检测板520的大小一致，且第一检测板510与第二检测板520同轴设置，第一检测板510的右端面与检测腔111的右端之间连接有第一推动杆530，第一检测板510的左端面设置有分光光度计发射装置。第一检测板510初始状态时处于最右侧滑动板210的右侧。第二检测板520的左端面与检测腔111的左端之间连接有第二推动杆540，第二检测板520的右端面设置有分光光度计接收装置。第二检测板520初始状态时处于最左侧滑动板210的左侧。第一推动杆530与第二推动杆540均能够伸缩设置，且第一推动杆530的伸长量与第二推动杆540的伸长量均能够单独控制，在对任意一组滑动板210上的多个试管600进行检测时，试管600与第一检测板510或第二检测板520的距离均相同，确保对试管600的检测精度。The detection device includes a first detection board 510 and a second detection board 520, the first detection board 510 and the second detection board 520 are both slidably arranged in the detection chamber 111, the first detection board 510 is arranged on the right side of the detection chamber 111, the second The second detection board 520 is arranged on the left side of the detection chamber 111, the size of the first detection board 510 and the second detection board 520 are consistent, and the first detection board 510 and the second detection board 520 are coaxially arranged, the first detection board 510 A first push rod 530 is connected between the right end surface and the right end of the detection cavity 111 , and a spectrophotometer emission device is arranged on the left end surface of the first detection plate 510 . The first detection board 510 is at the right side of the rightmost sliding board 210 in an initial state. A second push rod 540 is connected between the left end surface of the second detection plate 520 and the left end of the detection cavity 111 , and a spectrophotometer receiving device is disposed on the right end surface of the second detection plate 520 . The second detection board 520 is at the left side of the leftmost sliding board 210 in an initial state. Both the first push rod 530 and the second push rod 540 can be telescopically arranged, and the elongation of the first push rod 530 and the elongation of the second push rod 540 can be controlled separately. When a plurality of test tubes 600 are tested, the distances between the test tubes 600 and the first detection plate 510 or the second detection plate 520 are the same, so as to ensure the detection accuracy of the test tubes 600 .
在本实施例中，检测壳110上设置有显示器170，第二检测板520上的分光光度计接收装置通过信号分析后在显示器170上显示相关数据。In this embodiment, the detection shell 110 is provided with a display 170 , and the spectrophotometer receiving device on the second detection board 520 displays relevant data on the display 170 after signal analysis.
结合上述实施例，本发明工作过程如下：In conjunction with the foregoing embodiments, the working process of the present invention is as follows:
工作时，将封堵第一开口的盖板113打开，通过同时启动三个第二气缸220，三个第二气缸220推动三组滑动板210滑动至导向槽114的最上端。将多个方形试管600安装于安装块230的安装槽231内，同时通过打开三个进水管150，并封堵三个排水管，通过进水管150向三个控温腔121内注入液体，同时控制三个控温腔121内的加热丝140，调整每个控温腔121内液体的温度。将添加剂和底物添加至试管600内，同时向试管600内部添加调整PH值的缓冲剂，对影响添加剂活性的三个主要变量进行调整时，控制添加剂与物料的比例、温度、缓冲剂其中一个为定量，将另外两个变量进行调整，且处于同于同一组滑动板210上的多个试管600所处的温度相同。When working, the cover plate 113 blocking the first opening is opened, and the three second cylinders 220 are activated simultaneously, and the three second cylinders 220 push the three groups of sliding plates 210 to slide to the uppermost end of the guide groove 114 . A plurality of square test tubes 600 are installed in the installation groove 231 of the installation block 230, and at the same time, by opening three water inlet pipes 150, and blocking three drain pipes, inject liquid into the three temperature control chambers 121 through the water inlet pipes 150, and at the same time Control the heating wires 140 in the three temperature control chambers 121 to adjust the temperature of the liquid in each temperature control chamber 121 . Add the additive and the substrate to the test tube 600, and add a buffer to adjust the pH value inside the test tube 600. When adjusting the three main variables that affect the activity of the additive, one of the ratio of the additive to the material, the temperature, and the buffer is controlled. For quantification, the other two variables were adjusted and the temperature of the test tubes 600 on the same set of slide plates 210 was the same.
随着添加剂、底物和缓冲剂的加入试管600，再次启动第二气缸220，第二气缸220拉动滑动板210沿导向槽114向下滑动，使得试管600穿过保温板130上的通孔逐渐进入控温腔121内，在试管600进入控温腔121时，试管600挤压两个清洁块410相互远离，同时连接清洁块410与控温腔121侧壁的第一弹簧420被挤压。随着安装块230的下端进入控温腔121内，安装块230进一步挤压两个清洁块410相互远离，此时两个清洁块410抵接安装块230的侧壁。在滑动板210滑动至导向槽114的下端时启动第一电机320，第一电机320的动力输出轴上固定连接的传动齿轮321转动，传动齿轮321通过传动带310驱动多组安装块230同步转动，安装块230转动时，安装于安装槽231内的方形试管600同步转动，使得试管600内的试剂混合的更加均匀，进一步使得试管600内的各种试剂反应的更加充分。Along with the addition test tube 600 of additive, substrate and buffering agent, start second cylinder 220 again, second cylinder 220 pulls slide plate 210 and slides down along guide groove 114, makes test tube 600 pass through the through hole on the insulation plate 130 gradually Entering the temperature control cavity 121, when the test tube 600 enters the temperature control cavity 121, the test tube 600 squeezes the two cleaning blocks 410 away from each other, and the first spring 420 connecting the cleaning block 410 and the side wall of the temperature control cavity 121 is squeezed. As the lower end of the installation block 230 enters the temperature control cavity 121 , the installation block 230 further pushes the two cleaning blocks 410 away from each other, and at this time the two cleaning blocks 410 abut against the side wall of the installation block 230 . When the sliding plate 210 slides to the lower end of the guide groove 114, the first motor 320 is started, the transmission gear 321 fixedly connected on the power output shaft of the first motor 320 rotates, and the transmission gear 321 drives multiple sets of mounting blocks 230 to rotate synchronously through the transmission belt 310, When the mounting block 230 rotates, the square test tube 600 installed in the mounting groove 231 rotates synchronously, so that the reagents in the test tube 600 are mixed more evenly, and the various reagents in the test tube 600 are further reacted more fully.
在试管600内的各种试剂完全反应后，逐个关闭加热丝140对控温腔121内液体的加热，同时将连通控温腔121的排水管打开，将控温腔121内的液体排放出去，此时并不停止第一电机320的驱动，第一电机320的转动对附着于试管600侧壁的水珠进行甩动，水珠在离心力的作用下逐渐脱离试管600侧壁，此时停止该滑动板210上的第一电机320转动，并启动连接该滑动板210上的第二气缸220启动，第二气缸220推动该滑动板210沿导向槽114向上滑动，在滑动板210向上滑动时，两个清洁块410在两个第一弹簧420的作用下相互靠近，两个清洁块410能够纠正试管600的角度，使得方形试管600侧壁处于左右对称状态，在滑动板210上移时，同一组滑动板210上的多个试管600同步上移，且两个清洁块410对试管600的左右两侧壁的水渍进一步清理。此时启动第一推动杆530和第二推动杆540伸长，第一检测板510与第二检测板520逐渐靠近，第二检测板520的分光光度计接收装置通过信号分析后在显示器170上显示相关数据。After the various reagents in the test tube 600 have reacted completely, turn off the heating of the liquid in the temperature control chamber 121 by the heating wire 140 one by one, and simultaneously open the drain pipe connecting the temperature control chamber 121 to discharge the liquid in the temperature control chamber 121. At this time, the driving of the first motor 320 is not stopped, the rotation of the first motor 320 shakes the water drops attached to the side wall of the test tube 600, and the water drops gradually break away from the side wall of the test tube 600 under the action of the centrifugal force, at this time, stop the drive. The first motor 320 on the sliding plate 210 rotates and starts the second cylinder 220 connected to the sliding plate 210 to start. The second cylinder 220 pushes the sliding plate 210 to slide upward along the guide groove 114. When the sliding plate 210 slides upward, The two cleaning blocks 410 approach each other under the action of the two first springs 420. The two cleaning blocks 410 can correct the angle of the test tube 600, so that the side wall of the square test tube 600 is in a left-right symmetrical state. When the sliding plate 210 moves up, the same A plurality of test tubes 600 on the group sliding plate 210 move up synchronously, and two cleaning blocks 410 further clean the water stains on the left and right side walls of the test tubes 600 . Now start the first push rod 530 and the second push rod 540 to elongate, the first detection board 510 and the second detection board 520 approach gradually, and the spectrophotometer receiving device of the second detection board 520 is displayed on the display 170 after signal analysis Display related data.
由此往复对其余两组滑动板210上的多个试管600进行依次检测，并对相关数据进行分析，确定出其中一个定量外其余两个变量对添加剂活性的影响，其次，再次重复上述过程，调整其中一个定量为变量，且将其中一个变量为定量，再次对添加剂活性进行检测，对多组数据结合起来进行综合分析，进而确定该添加剂的活性处于最佳时三个变量的状态。Thus reciprocatingly detect the multiple test tubes 600 on the remaining two groups of sliding plates 210 in sequence, and analyze the relevant data to determine the influence of the other two variables on the activity of the additive except one of the quantitative ones, and then repeat the above process again, Adjust one of the quantifications as a variable, and one of the variables as a quantification, then test the activity of the additive again, combine multiple sets of data for a comprehensive analysis, and then determine the state of the three variables when the activity of the additive is at its best.
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of the present invention. within the scope of protection.
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