CN115508295B - A short-term test instrument for feed enzyme preparation activity - Google Patents
A short-term test instrument for feed enzyme preparation activity Download PDFInfo
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- CN115508295B CN115508295B CN202211334261.8A CN202211334261A CN115508295B CN 115508295 B CN115508295 B CN 115508295B CN 202211334261 A CN202211334261 A CN 202211334261A CN 115508295 B CN115508295 B CN 115508295B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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Abstract
The invention relates to the technical field of enzyme activity test, in particular to a rapid detection instrument for feed enzyme preparation activity, which comprises a detection shell, a sliding plate, an oscillating unit and a detection device, wherein an additive and a substrate are added into a test tube, a buffering agent for adjusting the PH value is added into the test tube, and a plurality of sliding plates arranged on the same group are arranged in the same temperature control cavity, so that the temperature in each temperature control cavity can be independently adjusted. When three main variables affecting the activity of the additive are detected, one quantification is controlled, the other two variables are regulated, meanwhile, the oscillating unit is started to enable substances added into the test tubes to be uniformly mixed, the detection of the activity of the additive is prevented from being affected by the mixing non-uniformity, a plurality of test tubes on a group of sliding plates are detected simultaneously, the first detection plates and the second detection plates are mutually close, and the distance between each group of sliding plates and the first detection plates is the same as the distance between each other and the second detection plates, so that the detection precision is improved.
Description
Technical Field
The invention relates to the technical field of enzyme activity test, in particular to a rapid detection instrument for feed enzyme preparation activity.
Background
The feed enzyme preparation is a feed additive, the common feed enzyme preparation is phytase, the phytase catalyzes phytic acid and phytate thereof to hydrolyze into inositol and phosphoric acid (or phosphate), and the feed additive can improve the digestion of animals on the feed or improve the metabolic efficiency in the animals when used in the feed.
The activity and concentration of the additive are critical factors for the quality and use effect of the product, and the reasons for influencing the activity of the additive mainly include reaction temperature, pH value of the mixed solution, concentration of the substrate and the like. In the detection process, according to the self characteristics of the additive, sodium phytate in a substrate is hydrolyzed under the conditions of a certain temperature and pH to generate orthophosphoric acid and inositol derivatives, and the orthophosphoric acid and inositol derivatives and vanadium ammonium molybdate are generated into yellow in an acidic solution, and the reaction rate is determined according to the color development of the vanadium ammonium molybdate in an acidic environment. According to the faster the additive catalyzed conversion rate, the higher the activity of the additive, and conversely, the slower the additive catalyzed conversion rate, the lower the activity of the additive.
When the activity of the additive is detected, the gradual comparison analysis is carried out through a plurality of groups of experiments, and when the plurality of groups of experiments are carried out, the stability and the identity of other variables are difficult to ensure, so that the error of the detection experimental data is large, and the state of the highest activity of the accurate feed enzyme preparation can not be found.
Disclosure of Invention
The invention provides a rapid detection instrument for the activity of a feed enzyme preparation, which aims to solve the problem of large activity detection error of the existing enzyme preparation.
The invention relates to a rapid detection instrument for feed enzyme preparation activity, which adopts the following technical scheme:
a rapid detection instrument for activity of a feed enzyme preparation comprises a detection shell, a sliding plate, an oscillating unit and a detection device;
the detection shell is provided with a detection cavity, the upper side wall of the detection shell is provided with a first opening communicated with the detection cavity, the lower side wall of the detection shell is fixedly connected with a temperature control shell, a plurality of independent temperature control cavities are arranged in the temperature control shell, and each group of temperature control cavities are communicated with the detection cavity; the sliding plates are provided with a plurality of groups, and each group of sliding plates can slide up and down in the detection cavity; each group of sliding plates is provided with a plurality of groups of mounting blocks, each group of mounting blocks is rotationally connected with the sliding plate, each group of mounting blocks is used for mounting square test tubes, and when the sliding plates slide up and down, a plurality of test tubes arranged on one group of sliding plates can enter the same temperature control cavity; the oscillating units are provided with a plurality of groups, and each group of oscillating units can drive a plurality of groups of mounting blocks on the same group of sliding plates to rotate; the detection device comprises a first detection plate and a second detection plate, wherein the first detection plate and the second detection plate are arranged on the left side and the right side of the plurality of groups of sliding plates in parallel, the first detection plate and the second detection plate can be slidably arranged in a detection cavity, the distance between the first detection plate and the second detection plate is the same from any group of sliding plates, a spectrophotometer transmitting device is arranged on the first detection plate, and a spectrophotometer receiving device is arranged on the second detection plate.
Further, the oscillating unit comprises a first power source and a driving belt, the peripheral side wall of each group of mounting blocks is provided with a plurality of tooth blocks, the first power source is fixedly arranged on the sliding plate, and the driving belt is sequentially wound on the power output shafts of the plurality of groups of mounting blocks and the first power source.
Further, each temperature control cavity is internally provided with a plurality of groups of cleaning assemblies, each group of cleaning assemblies comprises two cleaning blocks, the two cleaning blocks are arranged on two opposite side walls of the temperature control cavity through first elastic pieces, the side walls of the two cleaning blocks, which are close to each other, are arc surfaces, and the cleaning blocks are used for cleaning the side walls of the square test tubes.
Further, set up a plurality of inlet tubes and a plurality of drain pipes on the accuse temperature shell, every inlet tube intercommunication temperature control chamber, every drain pipe intercommunication temperature control chamber, every temperature control chamber bottom all is provided with the heater strip, and the heater strip is used for the liquid heating to the temperature control intracavity, makes the temperature homoenergetic of every temperature control intracavity independently adjust.
Further, the detection shell is provided with a plurality of groups of guide grooves penetrating inside and outside, each group of guide grooves extends up and down, and each group of sliding plates can slide up and down along the guide grooves; the outer side wall of the detection block is fixedly provided with a plurality of second power sources, a power output shaft of each second power source is fixedly connected with a group of sliding plates, and the second power sources are used for driving the sliding plates to slide up and down along the guide grooves.
Further, the installation piece is the round platform structure, and the one end that the installation piece diameter is big is in the one end top that the diameter is little, and a plurality of tooth pieces are along installation piece upper end lateral wall circumference evenly distributed, run through the sliding plate from top to bottom the installation piece, and every group installation piece all is provided with the square mounting groove that runs through from top to bottom.
Further, be provided with the heated board between detection chamber and the accuse temperature chamber, be provided with a plurality of through-holes that run through downwards on the heated board, the diameter of every through-hole is greater than square test tube cross-section's circumscribed circle diameter, and the diameter of through-hole is less than the diameter of installation piece upper end, when the sliding plate slides downwards, installation piece lower extreme can get into accuse temperature intracavity, and two cleaning blocks of lateral wall extrusion of installation piece are kept away from each other.
Further, a first pushing rod is connected to the first detection plate, and a second pushing rod is connected to the second detection plate.
Further, a cover plate for blocking the first opening is arranged on the detection shell.
The beneficial effects of the invention are as follows: the invention relates to a rapid detection instrument for activity of a feed enzyme preparation, which comprises a detection shell, sliding plates, an oscillating unit and a detection device, wherein a plurality of square test tubes are arranged on a fixed block, additives and substrates are gradually added into the test tubes, meanwhile, a buffering agent for adjusting the PH value is added into the test tubes, a plurality of test tubes arranged on the same group of sliding plates are positioned in the same temperature control cavity, and the temperature in each temperature control cavity can be independently adjusted, so that the test tubes on different groups of sliding plates can be positioned in different temperature environments. When detecting three main variables that influence the activity of additive, control a ration, adjust two other variables, start the oscillating unit simultaneously and make the material misce bene that adds to the test tube, prevent to mix inhomogeneous influence and produce the influence to the detection of additive activity, drive the sliding plate one by one and slide, detect a plurality of test tubes on a set of sliding plate simultaneously, and when every sliding plate of group upwards slides, first pick-up plate and second pick-up plate all can be close to each other for every sliding plate of group is the same with the distance of second pick-up plate apart from first pick-up plate, and then improves the detection precision.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the invention, and that other drawings can be obtained according to these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a schematic diagram of a rapid detection apparatus for feed enzyme preparation activity according to an embodiment of the present invention;
FIG. 2 is a top view of a rapid assay device for feed enzyme preparation activity according to an embodiment of the present invention with the cover plate removed;
FIG. 3 is a cross-sectional view taken along the direction A-A in FIG. 2;
FIG. 4 is a partial enlarged view at B in FIG. 3;
FIG. 5 is a schematic structural view of a rapid detection instrument for activity of a feed enzyme preparation, in which structures such as a detection shell and a temperature control shell are omitted;
FIG. 6 is a schematic diagram showing the structure of a group of slide plates, heating wires, etc. in a rapid detection apparatus for activity of a feed enzyme preparation according to an embodiment of the present invention;
FIG. 7 is a schematic diagram showing the structure of a mounting block in a rapid assay device for feed enzyme preparation activity according to an embodiment of the present invention.
In the figure: 110. a detection shell; 111. a detection chamber; 113. a cover plate; 114. a guide groove; 120. a temperature control shell; 121. a temperature control cavity; 130. a thermal insulation board; 140. a heating wire; 150. a water inlet pipe; 170. a display; 210. a sliding plate; 220. a second cylinder; 230. a mounting block; 231. a mounting groove; 232. tooth blocks; 310. a transmission belt; 320. a first motor; 321. a transmission gear; 410. a cleaning block; 420. a first spring; 510. a first detection plate; 520. a second detection plate; 530. a first push rod; 540. a second push rod; 600. and (5) a test tube.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The numbering of components herein, such as "first," "second," etc., is used merely to distinguish between the described objects and does not have any sequential or technical meaning. The terms "coupled" and "connected," as used herein, are intended to encompass both direct and indirect coupling (coupling), unless otherwise indicated. In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element in question must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature "above," "over" and "on" a second feature may be a first feature directly above or obliquely above the second feature, or simply indicate that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
An embodiment of a rapid assay device for feed enzyme preparation activity of the present invention, as shown in FIGS. 1 to 7, comprises an assay housing 110, a slide plate 210, an oscillation unit, and an assay device.
The detection shell 110 is provided with a detection cavity 111, the detection shell 110 is of a cuboid structure, the detection shell 110 is horizontally arranged, the detection shell 110 extends leftwards and rightwards, the detection shell 110 is hollow and is provided with the detection cavity 111, the upper end face of the detection shell 110 is provided with a first opening communicated with the detection cavity 111, and the detection shell 110 is provided with a cover plate 113 for blocking the first opening. The middle part of the lower end surface of the detection shell 110 is fixedly connected with a temperature control shell 120, the temperature control shell 120 is rectangular, the front-back width of the temperature control shell 120 is consistent with the front-back width of the detection shell 110, and the horizontal section size of the temperature control shell 120 is consistent with the section size of the cover plate 113. At least three independent temperature control cavities 121 are arranged in the temperature control shell 120, the three temperature control cavities 121 are uniformly distributed left and right in the temperature control shell 120, and each group of temperature control cavities 121 are communicated with the detection cavity 111. Specifically, an insulation board 130 is fixedly arranged between the temperature control cavity 121 and the detection cavity 111, the insulation board 130 above each temperature control cavity 121 is provided with a plurality of through holes penetrating from top to bottom, the through holes are used for communicating the detection cavity 111 and the temperature control cavity 121, and the plurality of through holes communicating the same temperature control cavity 121 and the detection cavity 111 are uniformly distributed on the insulation board 130. The detection shell 110 is provided with at least three groups of guide grooves 114 penetrating front and back, each group of guide grooves 114 are vertically arranged, and the three groups of guide grooves 114 are uniformly distributed along the left and right length of the first opening.
The sliding plates 210 are at least provided with three groups, the sliding plates 210 are horizontally arranged, the sliding plates 210 extend forwards and backwards, and each group of sliding plates 210 are slidably arranged in one group of guide grooves 114, so that the middle part of the sliding plate 210 is positioned in the detection cavity 111. The rear sidewall of the detection housing 110 is provided with at least three sets of second power sources for driving the sliding plate 210 to slide up and down along the guide groove 114. Specifically, the second power source is a second air cylinder 220, each second air cylinder 220 is fixedly disposed on the rear side wall of the detection housing 110, the power output shaft of each second air cylinder 220 is fixedly connected to the rear end of a group of sliding plates 210, and when the second air cylinders 220 are started, the second air cylinders 220 push the sliding plates 210 to slide up and down along the guide grooves 114. Every all be provided with a plurality of mounting holes that run through from top to bottom on the sliding plate 210 that set up, a plurality of mounting holes are along the length direction evenly distributed of sliding plate 210, all rotate in every mounting hole and are connected with a set of installation piece 230, all be provided with the mounting groove 231 that runs through from top to bottom on every installation piece 230 of group, every mounting groove 231 is the square groove, square test tube 600 can insert in the mounting groove 231, installation piece 230 is used for fixing square test tube 600. Each group of installation blocks 230 is of a round platform structure, each group of installation blocks 230 is vertically arranged, the middle part of each group of installation blocks 230 is rotationally connected with the side wall of the installation groove 231, the upper ends of the installation blocks 230 are located above the sliding plate 210, and the lower ends of the installation blocks 230 are located below the sliding plate 210. The diameter of the upper end of each group of mounting blocks 230 is larger than that of the lower end, the mounting grooves 231 coaxially penetrate through the mounting blocks 230, the diameter of the through holes in the heat insulation plate 130 is larger than that of the circumscribed circle of the horizontal section of the square test tube 600, the diameter of the through holes is larger than that of the bottommost end of the mounting blocks 230, and the diameter of the through holes is smaller than that of the topmost end of the mounting blocks 230.
The oscillating units are provided with at least three groups, and each group of oscillating units can drive the plurality of groups of mounting blocks 230 on the same group of sliding plates 210 to rotate. Specifically, each set of oscillating units includes a first power source and a driving belt 310, a plurality of tooth blocks 232 are uniformly disposed at the upper end of each set of mounting blocks 230 in the circumferential direction, the first power source is a first motor 320, and a driving gear 321 is fixedly disposed on a power output shaft of the first motor 320. Each first motor 320 is fixedly arranged at the front end of one group of sliding plates 210, the transmission gear 321 and the mounting blocks 230 are positioned at the same water height, the transmission belt 310 sequentially winds a plurality of groups of mounting blocks 230 and one transmission gear 321, when the first motor 320 is started, the plurality of groups of mounting blocks 230 on the same group of sliding plates 210 synchronously rotate, and the mounting blocks 230 drive the square test tubes 600 to synchronously rotate, so that reagents added into the test tubes 600 are uniformly mixed.
In this embodiment, at least three water inlet pipes 150 and three water outlet pipes are disposed on the temperature control shell 120, each water inlet pipe 150 is disposed on the front side wall of the temperature control shell 120, each water outlet pipe is disposed on the rear side wall of the temperature control shell 120, each water inlet pipe 150 is communicated with one temperature control cavity 121, each water inlet pipe 150 can inject water into the temperature control cavity 121, each water outlet pipe is communicated with one temperature control cavity 121, and each water outlet pipe can discharge water in the temperature control cavity 121. Each temperature control cavity 121 is internally provided with a heating wire 140, the heating wire 140 can heat the liquid temperature in the temperature control cavity 121, and each heating wire 140 can be independently controlled, so that the temperature in each temperature control cavity 121 can be independently adjusted.
In this embodiment, a plurality of groups of cleaning components are disposed in each temperature control chamber 121, and each group of cleaning components is disposed in one-to-one correspondence with a plurality of groups of mounting blocks 230 on the same sliding plate 210, and is used for cleaning water stains attached to a sidewall of a test tube 600. Specifically, each group of cleaning components includes two cleaning blocks 410, the two cleaning blocks 410 are of hemispherical block structures, and the two cleaning blocks 410 are disposed on the opposite left and right sidewalls of the temperature control cavity 121 through first elastic members, and the two cleaning blocks 410 can slide left and right in the temperature control cavity 121. Specifically, the first elastic member is a first spring 420, when the first spring 420 is in the original length, the two cleaning blocks 410 are abutted against each other, two side walls of the two cleaning blocks 410, which are close to each other, are arc surfaces, when the sliding plate 210 slides to the lower end of the guide groove 114, the lower end of the mounting block 230 enters the temperature control cavity 121, the side walls of the mounting block 230 press the two cleaning blocks 410 to be far away from each other, and the two cleaning blocks 410 are abutted against the side walls of the mounting block 230 under the action of the first spring 420, and when the test tube 600 rotates, the two cleaning blocks 410 slide along the side walls of the mounting block 230 in the circumferential direction.
The detection device comprises a first detection plate 510 and a second detection plate 520, wherein the first detection plate 510 and the second detection plate 520 are both arranged in the detection cavity 111 in a sliding manner, the first detection plate 510 is arranged on the right side of the detection cavity 111, the second detection plate 520 is arranged on the left side of the detection cavity 111, the first detection plate 510 and the second detection plate 520 are consistent in size, the first detection plate 510 and the second detection plate 520 are coaxially arranged, a first push rod 530 is connected between the right end face of the first detection plate 510 and the right end of the detection cavity 111, and a spectrophotometer emitter is arranged on the left end face of the first detection plate 510. The first detecting plate 510 is positioned on the right side of the rightmost slide plate 210 in the initial state. A second push rod 540 is connected between the left end face of the second detection plate 520 and the left end of the detection cavity 111, and the right end face of the second detection plate 520 is provided with a spectrophotometer receiving device. The second sensing plate 520 is positioned at the left side of the leftmost sliding plate 210 in the initial state. The first push rod 530 and the second push rod 540 can be set in a telescopic manner, and the elongation of the first push rod 530 and the elongation of the second push rod 540 can be controlled independently, so that when a plurality of test tubes 600 on any group of sliding plates 210 are detected, the distances between the test tubes 600 and the first detection plate 510 or the second detection plate 520 are the same, and the detection precision of the test tubes 600 is ensured.
In this embodiment, the display 170 is disposed on the detection housing 110, and the spectrophotometer receiving device on the second detection board 520 displays the relevant data on the display 170 after signal analysis.
In combination with the above embodiment, the working process of the invention is as follows:
in operation, the cover plate 113 blocking the first opening is opened, and the three second cylinders 220 push the three sets of sliding plates 210 to slide to the uppermost end of the guide groove 114 by simultaneously activating the three second cylinders 220. A plurality of square test tubes 600 are installed in the installation groove 231 of the installation block 230, simultaneously three water inlet pipes 150 are opened, three water outlet pipes are plugged, liquid is injected into the three temperature control cavities 121 through the water inlet pipes 150, meanwhile, the heating wires 140 in the three temperature control cavities 121 are controlled, and the temperature of the liquid in each temperature control cavity 121 is adjusted. When the additive and the substrate are added into the test tube 600 and the buffer for adjusting the PH value is added into the test tube 600, and three main variables affecting the activity of the additive are adjusted, one of the proportion of the additive and the material, the temperature and the buffer is controlled to be quantitative, and the other two variables are adjusted and are at the same temperature as the temperature of the plurality of test tubes 600 on the same group of sliding plates 210.
Along with the addition of the additives, the substrate and the buffer into the test tube 600, the second cylinder 220 is started again, and the second cylinder 220 pulls the sliding plate 210 to slide downwards along the guide groove 114, so that the test tube 600 gradually enters the temperature control cavity 121 through the through hole on the heat insulation plate 130, and when the test tube 600 enters the temperature control cavity 121, the test tube 600 presses the two cleaning blocks 410 away from each other, and simultaneously the first spring 420 connecting the cleaning blocks 410 with the side wall of the temperature control cavity 121 is pressed. As the lower end of the mounting block 230 enters the temperature control chamber 121, the mounting block 230 further presses the two cleaning blocks 410 away from each other, at which time the two cleaning blocks 410 abut against the side walls of the mounting block 230. When the sliding plate 210 slides to the lower end of the guide groove 114, the first motor 320 is started, the transmission gear 321 fixedly connected to the power output shaft of the first motor 320 rotates, the transmission gear 321 drives the plurality of groups of mounting blocks 230 to synchronously rotate through the transmission belt 310, and when the mounting blocks 230 rotate, the square test tube 600 mounted in the mounting groove 231 synchronously rotates, so that reagents in the test tube 600 are mixed more uniformly, and various reagents in the test tube 600 react more fully.
After various reagents in the test tube 600 completely react, the heating wire 140 is closed one by one to heat the liquid in the temperature control cavity 121, meanwhile, a drain pipe communicated with the temperature control cavity 121 is opened, the liquid in the temperature control cavity 121 is discharged, at the moment, the driving of the first motor 320 is not stopped, the rotation of the first motor 320 throws water drops attached to the side wall of the test tube 600, the water drops gradually separate from the side wall of the test tube 600 under the action of centrifugal force, at the moment, the rotation of the first motor 320 on the sliding plate 210 is stopped, and the second cylinder 220 connected with the sliding plate 210 is started, the second cylinder 220 pushes the sliding plate 210 to slide upwards along the guide groove 114, when the sliding plate 210 slides upwards, the two cleaning blocks 410 are mutually close under the action of the two first springs 420, the angles of the test tube 600 can be corrected, the side wall of the square test tube 600 is in a bilateral symmetry state, when the sliding plate 210 moves upwards, the plurality of test tubes 600 on the same group of the sliding plate 210 synchronously move upwards, and the two cleaning blocks 410 further clean water stains on the left side wall and the right side wall of the test tube 600. At this time, the first push rod 530 and the second push rod 540 are started to extend, the first detection plate 510 and the second detection plate 520 gradually approach each other, and the spectrophotometer receiving device of the second detection plate 520 displays the relevant data on the display 170 after signal analysis.
The test tubes 600 on the other two sets of sliding plates 210 are sequentially detected in a reciprocating manner, related data are analyzed, the influence of one of the other two variables except one of the variables on the activity of the additive is determined, then the process is repeated again, one of the variables is adjusted to be the variable, one of the variables is the variable, the activity of the additive is detected again, multiple sets of data are combined for comprehensive analysis, and the states of the three variables when the activity of the additive is optimal are determined.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (4)
1. A rapid detection instrument for activity of feed enzyme preparation is characterized in that: comprising the following steps:
the detection shell is provided with a detection cavity, the upper side wall of the detection shell is provided with a first opening communicated with the detection cavity, the lower side wall of the detection shell is fixedly connected with a temperature control shell, a plurality of independent temperature control cavities are arranged in the temperature control shell, and each group of temperature control cavities are communicated with the detection cavity;
the sliding plates are provided with a plurality of groups, and each group of sliding plates can slide up and down in the detection cavity; each group of sliding plates is provided with a plurality of groups of mounting blocks, each group of mounting blocks is rotationally connected with the sliding plate, each group of mounting blocks is used for mounting square test tubes, and when the sliding plates slide up and down, a plurality of test tubes arranged on one group of sliding plates can enter the same temperature control cavity;
the oscillating unit is provided with a plurality of groups, each group of oscillating units can drive a plurality of groups of mounting blocks on the same group of sliding plates to rotate, each oscillating unit comprises a first power source and a driving belt, the peripheral side wall of each group of mounting blocks is provided with a plurality of tooth blocks, the first power source is fixedly arranged on the sliding plates, the driving belt is sequentially wound on power output shafts of the plurality of groups of mounting blocks and the first power source, each mounting block is in a circular truncated cone structure, one end with a large diameter of the mounting block is positioned above one end with a small diameter, the plurality of tooth blocks are uniformly distributed along the peripheral direction of the side wall of the upper end of the mounting block, the mounting block vertically penetrates through the sliding plates, and each group of mounting blocks is provided with a square mounting groove which vertically penetrates through;
the detection device comprises a first detection plate and a second detection plate, wherein the first detection plate and the second detection plate
The detection plates are arranged on the left side and the right side of the plurality of groups of sliding plates in parallel, detection cavities are formed in the first detection plates and the second detection plates in a sliding manner, the distances between the first detection plates and the second detection plates are the same from any group of sliding plates, a spectrophotometer emitter is arranged on the first detection plates, a spectrophotometer receiver is arranged on the second detection plates, a plurality of groups of cleaning components are arranged in each temperature control cavity, each group of cleaning components comprises two cleaning blocks, the two cleaning blocks are arranged on two opposite side walls of the temperature control cavity through first elastic pieces, the side walls of the two cleaning blocks, which are close to each other, are all arc surfaces, the cleaning blocks are used for cleaning the side walls of square test tubes, a heat preservation plate is arranged between the detection cavities and the temperature control cavities, a plurality of downward penetrating through holes are formed in the heat preservation plates, the diameter of each through hole is larger than the diameter of an external circle of the square test tube, the diameter of each through hole is smaller than the diameter of the upper end of each mounting block, when the sliding plates slide downwards; the detection shell is provided with a plurality of groups of guide grooves which penetrate inside and outside, each group of guide grooves extends up and down, and each group of sliding plates can slide up and down along the guide grooves; the outer side wall of the detection block is fixedly provided with a plurality of second power sources, a power output shaft of each second power source is fixedly connected with a group of sliding plates, and the second power sources are used for driving the sliding plates to slide up and down along the guide grooves; the second cylinder promotes this sliding plate and upwards slides along the guide way, when the sliding plate upwards slides, two cleaning blocks are close to each other under the effect of two first springs, and the angle of test tube can be corrected to two cleaning blocks for square test tube lateral wall is in bilateral symmetry state, and when the sliding plate moved up, a plurality of test tubes on the same group of sliding plate moved up in step, and two cleaning blocks further clear up the water stain of the left and right sides wall of test tube.
2. A rapid assay device for feed enzyme preparation activity according to claim 1, wherein: the temperature control shell is provided with a plurality of water inlet pipes and a plurality of water outlet pipes, each water inlet pipe is communicated with one temperature control cavity, each water outlet pipe is communicated with one temperature control cavity, the bottom of each temperature control cavity is provided with a heating wire, and the heating wires are used for heating liquid in the temperature control cavities, so that the temperature in each temperature control cavity can be independently adjusted.
3. A rapid assay device for feed enzyme preparation activity according to claim 1, wherein: the first detection plate is connected with a first push rod, and the second detection plate is connected with a second push rod.
4. A rapid assay device for feed enzyme preparation activity according to claim 1, wherein: the detection shell is provided with a cover plate for blocking the first opening.
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| CN202211334261.8A CN115508295B (en) | 2022-10-28 | 2022-10-28 | A short-term test instrument for feed enzyme preparation activity |
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| CN202211334261.8A CN115508295B (en) | 2022-10-28 | 2022-10-28 | A short-term test instrument for feed enzyme preparation activity |
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| CN115508295B true CN115508295B (en) | 2023-08-01 |
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| CN112300911A (en) * | 2020-10-27 | 2021-02-02 | 广州和实生物技术有限公司 | Nucleic acid detector and nucleic acid detection method |
| CN214131407U (en) * | 2021-08-13 | 2021-09-07 | 山东海特环保科技有限公司 | Soil testing shakes even device with diluting |
| CN216696056U (en) * | 2021-12-07 | 2022-06-07 | 江苏中农生物科技有限公司 | Detection equipment for additive in egg product |
| CN216445359U (en) * | 2021-12-30 | 2022-05-06 | 山佳经典(北京)科技有限公司 | Compound fungus fermentation enzyme activity detection device |
| CN216825940U (en) * | 2022-03-11 | 2022-06-28 | 邢娜 | Test tube vibrating device for medicine inspection and detection |
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| CN206009373U (en) * | 2016-08-24 | 2017-03-15 | 高伟 | A kind of medicament test tubes cleaning device |
| CN109731872A (en) * | 2019-02-28 | 2019-05-10 | 白城师范学院 | A kind of biochemistry Multifunctional test tube rotary cleaning device |
| CN209953437U (en) * | 2019-04-19 | 2020-01-17 | 吴慧卿 | Degassing unit for clinical laboratory |
| CN211978493U (en) * | 2019-12-28 | 2020-11-20 | 深圳市帝迈生物技术有限公司 | Sample analyzer, automatic mixing device of blood sample |
| CN112077091A (en) * | 2020-09-08 | 2020-12-15 | 温州阳格凡电子科技有限公司 | Test tube cleaning equipment for experiments |
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