CN115500432A - Granules for preventing and treating bacterial diseases of livestock and poultry and preparation method thereof - Google Patents

Abstract

The invention relates to the field of feed additives, in particular to granules for preventing and treating bacterial diseases of livestock and poultry and a preparation method thereof. The granules for preventing and treating bacterial diseases of livestock and poultry comprise a component A and a component B, wherein the component A and the mass ratio thereof are as follows: per 1000g of A: 20g to 60g of nicotinamide, 20g to 60g of taurine, 80g to 120120g of astragalus extract, 5g to 15g of myrobalan extract, 5g to 15g of myristica fragrans extract, 5g to 15g of liquorice extract, 30g to 70g of berberine hydrochloride, 350g to 550g of glucose and 1000g of starch; b is a high molecular polymer aqueous solution with the concentration of 0.1-1%, and the dosage is as follows: 220mL to 350mL is added to 1000g of A. The invention prepares the vitamins and the traditional Chinese medicine formula into granules, is applied to feed additives, can improve the immunity of animals, regulate intestinal flora to prevent bacterial diseases, can also promote the ingestion of the animals and increase the weight of slaughtering; when the animal has bacterial diseases, the composition can be combined with antibacterial drugs, so that the dosage of the drugs can be reduced, and the recovery of the animals can be promoted; the contained high molecular polymer has slow release effect and can prolong the effective time.

Description

Granules for preventing and treating bacterial diseases of livestock and poultry and preparation method thereof

Technical Field

The invention relates to the field of feed additives, in particular to granules for preventing and treating bacterial diseases of livestock and poultry and a preparation method thereof.

Background

The disease control is particularly important in the process of breeding livestock and poultry, but in recent years, due to the wide and unreasonable use of antibacterial drugs, the problem of bacterial drug resistance is becoming more serious, and the drug resistance level of bacteria is continuously rising, and the development from single drug resistance to multi-drug resistance is shown, so that a plurality of drugs are ineffective in veterinary clinic use, if the use of the antibacterial drugs can be reduced in the breeding process, the generation of bacterial drug resistance is certainly reduced and delayed, the drug residue is reduced, and more green and healthy animal food is provided for human beings.

The traditional Chinese medicine is inherited for thousands of years in China, and also has the effect on preventing and treating infection. Researches show that a part of traditional Chinese medicines have good bacteriostatic effect when being used singly, and some traditional Chinese medicines have good synergistic effect when being used together with antibacterial medicines although the traditional Chinese medicines have no in-vitro antibacterial activity or low antibacterial activity, so that the antibacterial capability of the antibacterial medicines can be enhanced, and the drug resistance of bacteria can be reduced. In addition, some traditional Chinese medicines have the effects of enhancing the immunity of the organism and preventing diseases.

Vitamins are organic compounds necessary for nutrition and growth of human and animals, and play an important role in metabolism, growth, development and health of organisms.

Therefore, the research on the compound of the traditional Chinese medicine and the vitamins has important significance in the application of the compound in the feed additive for promoting the ingestion and the growth of the livestock and poultry, enhancing the resistance of the livestock and poultry, preventing the bacterial diseases of the livestock and poultry, reducing the use of antibacterial drugs in clinic and reducing the drug residue of animal food.

Disclosure of Invention

In view of the above, the invention aims to provide a compound of traditional Chinese medicines and vitamins, which is applied to a feed additive and used for promoting the ingestion and growth of livestock and poultry, enhancing the immunity of livestock and poultry, preventing bacterial diseases of livestock and poultry, reducing the use of antibacterial drugs and the drug residue of animal food in the livestock and poultry breeding process, and providing more green and healthy animal food for human beings.

In order to achieve the above purpose, the invention provides the following technical scheme:

a granule for preventing and treating bacterial diseases of livestock and poultry comprises A and B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A: .

Nicotinamide 20g to 60g

Taurine (20 g to 60g)

Radix astragali extract 80g to 120g

5g to 15g of myrobalan extract

5g to 15g of nutmeg extract

5g to 15g of licorice extract

30g to 70g of berberine hydrochloride

350g to 550g of glucose

Starch supplement to 1000g

B is a high molecular polymer aqueous solution with the concentration of 0.1-1%, and the dosage is as follows: 220mL to 350mL is added to 1000g of A.

Preferably, the granule for preventing and treating bacterial diseases of livestock and poultry consists of a part A and a part B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A:

nicotinamide 15g to 50g

Taurine 18g to 50g

90g to 100g of astragalus extract

Myrobalan extract 8g to 15g

Myristica fragrans extract (8g-12g)

Glycyrrhiza extract (6 g to 13g)

Berberine hydrochloride 40g to 60g

400g to 520g of glucose

Starch supplement to 1000g

B is a high molecular polymer aqueous solution with the concentration of 0.1-1%, and the dosage is as follows: 240mL to 330mL are added to 1000g of A.

Preferably, the granule for preventing and treating bacterial diseases of livestock and poultry consists of a part A and a part B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A:

nicotinamide 45g

Taurine 45g

Radix astragali extract 95g

12g of myrobalan extract

Myristica fragrans extract 11g

Glycyrrhrizae radix extract 12g

Berberine hydrochloride 55g

480g of glucose

Starch supplement to 1000g

B is 0.8% high molecular polymer water solution, and the dosage is: each 1000g of A was added to 260mL.

Further, the livestock and poultry bacterial diseases comprise diseases caused by one or more of escherichia coli, salmonella, staphylococcus aureus and klebsiella.

Further, the high molecular polymer is water soluble, such as one or more of acacia, tragacanth, sodium alginate, peach gum, carboxymethyl starch, acetate starch, methyl cellulose, sodium carboxymethyl cellulose, hydroxyethyl cellulose, and dextran.

Further, the aqueous solution of the high molecular polymer contains ethanol.

Further, the preparation method of the aqueous solution of the high molecular polymer comprises the following steps: weighing a high molecular polymer, adding 95% ethanol which is 2-6 times the weight of the high molecular polymer, fully stirring to completely disperse the high molecular polymer in the 95% ethanol to obtain a suspension, and adding purified water into the suspension to fix the volume to the concentration of the high molecular polymer to be 0.1-1%.

The invention also discloses a preparation method of the granules for preventing and treating bacterial diseases of livestock and poultry, which comprises the following steps:

(1) Mixing fructus Chebulae extract, semen Myristicae extract, and Glycyrrhrizae radix extract, adding nicotinamide and taurine, mixing, adding radix astragali extract, berberine hydrochloride, glucose, and starch, and mixing;

(2) Putting the mixture prepared in the step (1) into a wet granulation pot, spraying the aqueous solution of the high molecular polymer on the surface of the mixture for a few times, and stirring while spraying until the mixture is completely wetted to obtain a soft material;

(3) Preparing the soft material prepared in the step (2) into granules in a swing granulator;

(4) And (3) transferring the prepared granules to an oven for drying at the drying temperature of 60 ℃, detecting the moisture (the moisture should be 3-6%), grading after the moisture is detected to be qualified, and packaging.

The invention has the beneficial effects that: the invention prepares the vitamins and the traditional Chinese medicine into granules, is applied to feed additives, and takes taurine as a growth promoting factor of bifidobacterium, can optimize the intestinal flora structure and has a protective effect on kidney; nicotinamide is a raw material for synthesizing coenzyme I and coenzyme II, and plays an important role in the metabolism of sugar, fat and protein and the formation of high-energy bonds in biological oxidation; the myrobalan has the effects of relieving diarrhea with astringents and astringing lung to relieve cough, and research shows that the myrobalan has a certain antibacterial effect; nutmeg can be used for treating diarrhea and dysentery, and promoting qi circulation and digestion, and is good for promoting ingestion; the radix astragali extract can improve the activity of immunocyte of organism, and has effects of enhancing immunity, enhancing energy, and relieving fatigue; research shows that the combination of the liquorice, the berberine hydrochloride and the antibacterial drug has a synergistic effect and can increase the activity of the drug-resistant bacteria on the antibacterial drug. The vitamins and the traditional Chinese medicine formula are added into daily ration or drinking water of livestock and poultry, so that the immunity of animal organisms can be improved, the disease resistance can be enhanced, intestinal flora can be adjusted, bacterial diseases can be prevented, meanwhile, the ingestion of the animals can be promoted, and the slaughter weight of the animals can be increased; when the animal has bacterial diseases, the antibacterial composition can be combined with antibacterial drugs for application, so that the use amount of the antibacterial drugs can be reduced, and the animal rehabilitation is promoted; the added water-soluble high molecular polymer can play a role of slow release and prolong the time of the granules in exerting effect on animal organisms.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments of the present invention will be described in detail below.

Example 1

The granule for preventing and treating bacterial diseases of livestock and poultry comprises a component A and a component B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A:

nicotinamide 45g

Taurine 45g

Radix astragali extract 95g

12g of myrobalan extract

Myristica fragrans Houtt extract 11g

Glycyrrhiza extract 12g

Berberine hydrochloride 55g

480g of glucose

Starch supplement to 1000g

B is 0.8% high molecular polymer water solution, and the dosage is: each 1000g of A was added to 260mL.

The preparation method of the embodiment comprises the following steps:

the first step is as follows: weighing 2.08g of high molecular polymer, adding 8.32g of 95% ethanol, fully stirring to completely disperse the high molecular polymer in the 95% ethanol to obtain a suspension, and adding purified water into the suspension to make the volume to 260mL for later use.

The second step is that: (1) Mixing fructus Chebulae extract, semen Myristicae extract, and Glycyrrhrizae radix extract, adding nicotinamide and taurine, mixing, adding radix astragali extract, berberine hydrochloride, glucose, and starch, and mixing;

(2) Putting the mixture prepared in the step (1) into a wet granulation pot, spraying the aqueous solution of the high molecular polymer on the surface of the mixture for a few times, and stirring while spraying until the mixture is completely wetted to obtain a soft material;

(3) Putting the soft material prepared in the step (2) into a swinging granulator to prepare granules;

(4) And (3) transferring the prepared granules to an oven for drying at the drying temperature of 60 ℃, detecting the moisture (the moisture should be 3-6%), grading after the moisture is detected to be qualified, and packaging.

Example 2

The granule for preventing and treating bacterial diseases of livestock and poultry comprises a component A and a component B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A:

nicotinamide 50g

Taurine 50g

Radix astragali extract 90g

10g myrobalan extract

Myristica fragrans Houtt extract 12g

Glycyrrhiza extract 10g

Berberine hydrochloride 60g

Glucose 500g

Starch supplement to 1000g

B is a 1% high molecular polymer aqueous solution, and the dosage is as follows: 300mL of the solution was added per 1000g of A.

The preparation method of the embodiment comprises the following steps:

the first step is as follows: weighing 3g of high molecular polymer, adding 15g of 95% ethanol, fully stirring to completely disperse the high molecular polymer in the 95% ethanol to obtain a suspension, and adding purified water into the suspension to reach a constant volume of 300mL for later use.

The second step is that: (1) Mixing fructus Chebulae extract, semen Myristicae extract, and Glycyrrhrizae radix extract, adding nicotinamide and taurine, mixing, adding radix astragali extract, berberine hydrochloride, glucose, and starch, and mixing;

(2) Putting the mixture prepared in the step (1) into a wet granulation pot, spraying the aqueous solution of the high molecular polymer on the surface of the mixture for a few times, and stirring while spraying until the mixture is completely wetted to obtain a soft material;

(3) Preparing the soft material prepared in the step (2) into granules in a swing granulator;

(4) And (3) transferring the prepared granules to an oven for drying at the drying temperature of 60 ℃, detecting the moisture (the moisture should be 3-6%), grading after the moisture is detected to be qualified, and packaging.

Effect example 1

1. Test animal

In a certain meat duck farm, 600 healthy 1-day-old Gao ducks with the weight of 50g to 60g are randomly selected.

2. Test method

The ducklings are randomly divided into 2 groups of 300 ducklings.

Group A feeds formulated duck feed and clean drinking water.

Group B the granules prepared according to example 1 were mixed and drunk at 3 to 5 days old, 8 to 10 days old, 15 to 17 days old, 21 to 23 days old in a proportion of 1g of water per 1L, and the rest feeding modes were the same as those of group A.

The weight of the ducks is weighed at the age of 30 days, and the fatality rate of the two groups of ducks is recorded.

3. Results and analysis

The test results are shown in the following table

Group of	Birth average body weight (g)	Average body weight (g) at 30 days of age	Average body weight gain (g)	Rate of disease death
					A	57.63	1108.59	1050.96	5.67%
B	55.21	1246.87	1191.66	1.67%

From the test results, the addition of the granules prepared according to example 1 to drinking water can promote the growth of ducks, improve the weight of ducks and obviously reduce the fatality rate of ducks.

Effect example 2

1. Test animal

360 healthy 5-day-old black foot partridge chickens are selected from a certain chicken farm and are fed for 1 week and then grouped.

2. Test method

(1) Grouping: the chicks are randomly divided into 4 groups, namely, a group A, a group B, a group C and a group D, wherein the group A is a healthy control group, the group B is a negative control group, the group C is a positive control group, the group D is a test group, and 90 chicks are respectively arranged in each group.

(2) Molding method

The bacteria for molding are salmonella standard strain CVCC (B) 50335 which are purchased from the inspection institute of veterinary drugs in China.

Preparing bacterial liquid: inoculating the strain into common nutrient broth culture medium, performing shake culture at 37 deg.C for 18-24 h to obtain bacterial liquid, and diluting with sterile MH broth to 3 × 10 ⁸ cfu/ml of bacterial liquid.

Molding: chick pectoralis muscle injection 3X 10 ⁸ 0.5ml of cfu/ml salmonella liquid is observed for the clinical symptoms of the chicken every 1 h.

And (3) judging standard of successful molding: when the chickens are in full flock and have the symptoms of listlessness, head weakness and low drooping, blindness, diet reduction or even abolition, loose and disorderly feathers, white and thin feces discharge, excrement paste around the anus and the like, the molding success is indicated. After the model is successfully made, the medicine treatment is carried out.

The B, C and D groups were molded as described above, and the A group was injected with an equal amount of physiological saline.

(3) Feeding and administration method

Group A is a healthy control group, and is fed with formula chicken feed and clean drinking water.

Group B is negative control group, and is fed with formula chicken feed and clean drinking water.

And C group is a positive control group, and is fed with formula chicken feed, and the medicinal drinking water is administered according to the proportion of adding 0.75mL10% enrofloxacin solution into 1L water for 5 days continuously.

Group D was the test group, and the group was fed with a formula chicken feed, and medicated drinking water was administered for 5 days in a proportion of 0.375mL10% enrofloxacin solution and 0.25g of the granule prepared in example 2 per 1L of water.

Mortality was recorded on day 5 of dosing and day 7 of withdrawal.

3. Results recording and analysis

After the chicken is injected with the salmonella bacteria liquid for 2 hours, the chicken shows symptoms of listlessness, head weakness and low drop, blindness and poor standing, diet reduction and even abominability, loose and disorderly feathers and white and thin excrement discharge, which indicates that the molding is successful, and then the chicken is treated according to the administration method. The number of dead chickens on the 5 th day (17 days old) and 7 th day (24 days old) after drug administration is recorded, and the death rate and the treatment effective rate of the chickens are calculated.

Mortality = (number of dead chickens in group/initial total number of chickens in group) × 100%

Effective rate = 1-mortality

Mortality and treatment efficacy of the chickens are shown in the following table

Group of	Mortality on day 5 of administration (%)	Effective rate on day 5 of administration (%)	Mortality on day 7 of drug withdrawal (%)	Effective rate (%)% on day 7 after stopping taking medicine
					A	0.00	-	6.67	-
B	56.67	-	73.33	-
					C	16.67	83.33	20.00	80.00
D	16.67	83.33	16.67	83.33

From the test results, the effective rate of the group D is the same as that of the group C on the 5 th day of administration treatment, which shows that after the dosage of the enrofloxacin is halved, the granules can still achieve the treatment effect when the dosage is not halved after being combined with the granules, namely the granules can reduce the dosage of the enrofloxacin after being combined with the enrofloxacin; the mortality of the group C is increased after the medicine is stopped for 7 days, while the mortality of the group D is not increased, which shows that the combination of the granules and the enrofloxacin can last longer for the treatment of animal organisms, namely the granules have certain slow release.

Finally, it is noted that the above-mentioned embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (8)

1. A granule for preventing and treating bacterial diseases of livestock and poultry is characterized in that: the preparation consists of a part A and a part B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A: .

Nicotinamide 20g to 60g

Taurine (20 g to 60g)

Radix astragali extract 80g to 120g

5g to 15g of myrobalan extract

5g to 15g of myristica fragrans extract

5g to 15g of licorice extract

30g to 70g of berberine hydrochloride

350g to 550g of glucose

Starch supplement to 1000g

B is a high molecular polymer aqueous solution with the concentration of 0.1-1%, and the dosage is as follows: 220mL to 350mL is added to 1000g of A.

2. The granule for preventing and treating bacterial diseases of livestock and poultry according to claim 1, which is characterized in that: the preparation consists of a part A and a part B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A:

nicotinamide 15g to 50g

Taurine 18g to 50g

90g to 100g of astragalus extract

Myrobalan extract 8g to 15g

Myristica fragrans extract (8g-12g)

Glycyrrhiza extract (6 g to 13g)

Berberine hydrochloride 40g to 60g

400g to 520g of glucose

Starch supplement to 1000g

B is a high molecular polymer aqueous solution with the concentration of 0.1-1%, and the dosage is as follows: 240mL to 330mL are added to 1000g of A.

3. The granule for preventing and treating bacterial diseases of livestock and poultry according to claim 1, which is characterized in that: the preparation consists of a part A and a part B, and specifically comprises the following components:

the component A and the mass ratio thereof are as follows:

per 1000g of A:

nicotinamide 45g

Taurine 45g

Astragalus extract 95g

12g myrobalan extract

Myristica fragrans extract 11g

Glycyrrhiza extract 12g

Berberine hydrochloride 55g

480g of glucose

Starch supplement to 1000g

B is 0.8% high molecular polymer water solution, and the dosage is: each 1000g of A was added to 260mL.

4. The granule for preventing and treating bacterial diseases of livestock and poultry according to claim 1, which is characterized in that: the bacterial diseases of the livestock and poultry comprise diseases caused by one or more of escherichia coli, salmonella, staphylococcus aureus and klebsiella.

5. The granule for preventing and treating bacterial diseases of livestock and poultry according to any one of claims 1 to 3, characterized in that: the high molecular polymer is water soluble, such as acacia, tragacanth, sodium alginate, peach gum, carboxymethyl starch, acetate starch, methyl cellulose, sodium carboxymethyl cellulose, hydroxyethyl cellulose, dextran, etc.

6. The granule for preventing and treating bacterial diseases of livestock and poultry according to any one of claims 1 to 3, which is characterized in that: the aqueous solution of the high molecular polymer contains ethanol.

7. The granule for preventing and treating bacterial diseases of livestock and poultry according to any one of claims 1 to 3, which is characterized in that: the preparation method of the aqueous solution of the high molecular polymer comprises the following steps: weighing a high molecular polymer, adding 95% ethanol with the weight 2-6 times of that of the high molecular polymer, fully stirring to completely disperse the high molecular polymer in the 95% ethanol to obtain a suspension, and adding purified water into the suspension to fix the volume to the concentration of the high molecular polymer of 0.1-1%.

8. The granule for preventing and treating bacterial diseases of livestock and poultry, as claimed in any one of claims 1 to 3, is prepared by the following steps: (1) Mixing fructus Chebulae extract, semen Myristicae extract, and Glycyrrhrizae radix extract, adding nicotinamide and taurine, mixing, adding radix astragali extract, berberine hydrochloride, glucose, and starch, and mixing;

(2) Putting the mixture prepared in the step (1) into a wet granulation pot, spraying the aqueous solution of the high molecular polymer on the surface of the mixture for a few times, and stirring while spraying until the mixture is completely wetted to obtain a soft material;

(3) Preparing the prepared soft material into granules in a swing granulator;

(4) And (3) transferring the prepared granules to an oven for drying at the drying temperature of 60 ℃, detecting the moisture (the moisture should be 3-6%), grading after the moisture is detected to be qualified, and packaging.