CN115487339A - Micro-crosslinked spongy wound dressing and preparation method thereof - Google Patents
Micro-crosslinked spongy wound dressing and preparation method thereof Download PDFInfo
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
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- A—HUMAN NECESSITIES
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/406—Antibiotics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2429/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal, or ketal radical; Hydrolysed polymers of esters of unsaturated alcohols with saturated carboxylic acids; Derivatives of such polymer
- C08J2429/02—Homopolymers or copolymers of unsaturated alcohols
- C08J2429/04—Polyvinyl alcohol; Partially hydrolysed homopolymers or copolymers of esters of unsaturated alcohols with saturated carboxylic acids
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/07—Aldehydes; Ketones
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a micro-crosslinked spongy wound dressing and a preparation method thereof, wherein the dressing comprises a first component and a second component; the first component comprises: according to the parts by weight, 10-20 parts of polyvinyl alcohol, 30-45 parts of chitosan and 30-45 parts of gelatin; the second component comprises an antibacterial agent, glycerol and glutaraldehyde; wherein the mass of the glutaraldehyde accounts for 0.5-5% of the total mass of the first component. According to the invention, through adding glutaraldehyde, the slow release time is prolonged, and the bacteriostatic period is improved. According to the invention, chitosan, gelatin and polyvinyl alcohol are compounded, ciprofloxacin hydrochloride is added to inhibit bacterial growth, and glutaraldehyde serving as a cross-linking agent is added to further improve the antibacterial period and mechanical strength of the material, so that the effects of protecting a wound surface and treating the wound are achieved.
Description
Technical Field
The invention relates to a dressing and a preparation method thereof, in particular to a micro-crosslinked spongy wound dressing and a preparation method thereof.
Background
The traditional wound treatment material mainly comprises absorbent cotton, gauze or a bandage, and the medicine is loaded on the wound treatment material in a liquid or semi-solid state form, so that the wound treatment material plays a role in debridement and pain relief, and simultaneously has the problems of secondary injury of the wound, insufficient hemostatic capacity and the like caused by easy adhesion in the healing process.
Chen Liping prepares a chitosan/gelatin/polyvinyl alcohol ternary composite film, and antibacterial performance analysis is carried out on the composite film, so that the composite film has no antibacterial effect (Chen Liping. Preparation and performance optimization of a chitosan/PVA/gelatin composite film [ D ]. Harbin university of industry, 2011.). The blue guan Qian takes silkworm chrysalis chitosan and gelatin as raw materials to prepare a chitosan/gelatin composite hemostatic material, and antibacterial performance analysis is carried out on the material, so that the material shows weak antibacterial effect on staphylococcus aureus and escherichia coli (the blue guan Qian, the development of the chitosan/gelatin composite hemostatic material and performance research thereof [ D ] southwest university, 2016.). Li Xuan uses collagen and chitosan as raw materials, and utilizes an electrostatic spinning technology to prepare a collagen/chitosan dressing film, and antibacterial experimental analysis shows that the antibacterial zone of the dressing film to escherichia coli is 1mm (Li Xuan. Preparation and characterization of collagen/chitosan composite antibacterial dressing film [ D ]. Donghua university, 2017.).
After the skin is damaged, the wound is easy to be infected with bacteria, various complications are caused, and the pain is continuously brought to the patient. Therefore, on the basis of the research, the dressing which can quickly sterilize and can realize long-acting antibiosis needs to be developed, so that the aims of quickly healing wounds and relieving the pain of patients are fulfilled. To ameliorate these problems, several new wound dressings have been developed in succession. The chitosan as natural polymer material has good biocompatibility and biodegradability, and also has the functions of antibiosis, antiphlogosis, hemostasis and promotion of regeneration, repair and healing of wound tissues. Gelatin is a biomacromolecule protein extracted from animal skin and bone, and has the advantages of biodegradability, biocompatibility, low antigenicity and the like. The dressing made of chitosan and gelatin has good absorptivity, biocompatibility and degradability, but the mechanical property is relatively poor. Polyvinyl alcohol is a water-soluble artificially-synthesized high molecular material prepared by alcoholysis of polyvinyl acetate, molecular chains of the water-soluble artificially-synthesized high molecular material have a strictly regular linear structure, each repeating unit on the molecular chains contains a hydroxyl group, the polarity of the hydroxyl groups is strong, a large amount of intra-molecular and intermolecular hydrogen bonds are very easily formed, and the characteristics endow the polyvinyl alcohol with good water solubility, film-forming property and high crystallinity and chemical stability. The dressing prepared from polyvinyl alcohol has high water content, high mechanical strength, easy degradation, low cost and easy processing. Therefore, the wound dressing can be prepared by combining the excellent properties of the chitosan, the gelatin and the polyvinyl alcohol, so as to meet the complex requirement of the wound, but the dressing has the defect of poor antibacterial property.
Disclosure of Invention
The purpose of the invention is as follows: the invention aims to provide a micro-crosslinked spongy wound dressing with long bacteriostatic period and good antibacterial effect, which is prepared by compounding chitosan, gelatin and polyvinyl alcohol, adding ciprofloxacin hydrochloride to inhibit bacterial growth, and simultaneously adding a crosslinking agent glutaraldehyde to further improve the bacteriostatic period and mechanical strength of the material, so that the wound surface is protected and the wound is treated; the invention also aims to provide a simple and rapid preparation method of the micro-crosslinked spongy wound dressing.
The technical scheme is as follows: the invention relates to a micro-crosslinked spongy wound dressing, which comprises a first component and a second component; the first component comprises: according to the parts by weight, 10-20 parts of polyvinyl alcohol, 30-45 parts of chitosan and 30-45 parts of gelatin; the second component comprises an antibacterial agent, glycerol and glutaraldehyde; wherein the mass of the glutaraldehyde accounts for 0.5-5% of the total mass of the first component. According to the invention, through adding glutaraldehyde, the slow release time is prolonged, and the bacteriostatic period is improved.
Furthermore, the antibacterial agent is ciprofloxacin hydrochloride, and the antibacterial effect of the wound dressing is improved by adding the antibacterial agent.
Furthermore, the mass of the antibacterial agent is 0.008-0.032% of the total mass of the first component.
Further, the mass of the glycerol is 1-2% of the total mass of the first component.
In another aspect, the present invention provides a method for preparing the micro-crosslinked spongy wound dressing, which comprises the following steps:
(1) After the first component is uniformly mixed, sequentially adding an antibacterial agent, glycerol and glutaraldehyde, and stirring until the mixture is uniformly mixed to obtain a mixed solution;
(2) And (3) performing ultrasonic defoaming on the mixed solution, and coating the mixed solution on a glass plate to form a film to obtain the spongy wound dressing.
Further, in the step (1), the stirring time is 20-30 min.
Further, in the step (1), the stirring is magnetic stirring.
Has the beneficial effects that: compared with the prior art, the invention has the following remarkable advantages:
(1) The preparation method is simple, convenient and quick, achieves the synergistic effect by compounding natural materials and synthetic polymer materials, and accords with the development trend of medical wound dressings;
(2) The antibacterial agent is introduced in the process of preparing the dressing, so that the dressing can protect the wound surface and play a role in treating the wound;
(3) In the invention, the cross-linking agent is introduced in the process of preparing the dressing, so that the mechanical property of the dressing is further enhanced, and the bacteriostasis period is prolonged.
Drawings
FIG. 1 is a cross-sectional profile of a composite film, a being the cross-sectional profile of example 1 and b being the cross-sectional profile of comparative example 1;
FIG. 2 is a diagram of the antibacterial effect of the composite film: a1, a2, a3 and a4 are graphs of the antibacterial effect on staphylococcus aureus after the composite membrane prepared in the embodiment 1 slowly releases 0d, 7d, 14d and 21 d; b1, b2, b3 and b4 are graphs of the antibacterial effect of the composite membrane prepared in the comparative example 1 on staphylococcus aureus after 0d, 7d, 14d and 21d of slow release;
FIG. 3 is a diagram of the antibacterial effect of the composite membrane: c1, c2, c3 and c4 are graphs of the antibacterial effect of the composite membrane prepared in the embodiment 2 on staphylococcus aureus after the composite membrane slowly releases 0d, 7d, 14d and 21d, d1, d2, d3 and d4 are graphs of the antibacterial effect of the composite membrane prepared in the embodiment 3 on staphylococcus aureus after the composite membrane slowly releases 0d, 7d, 14d and 21d, and e1 is a graph of the antibacterial effect of the composite membrane prepared in the comparative example 2 on staphylococcus aureus after the composite membrane slowly releases 0 d.
Detailed Description
The technical scheme of the invention is further explained by combining the attached drawings.
Example 1
This example provides a micro-crosslinked sponge wound dressing comprising a first component and a second component; the first component comprises: according to the parts by weight, 10 parts of polyvinyl alcohol, 30 parts of chitosan and 30 parts of gelatin; the second component comprises an antibacterial agent, glycerol and glutaraldehyde; wherein the mass of the glutaraldehyde accounts for 0.5 percent of the total mass of the first component; the mass of the antibacterial agent is 0.0032% of the total mass of the first component, and the mass of the glycerol is 1.26% of the total mass of the first component.
The embodiment provides a preparation method of a micro-crosslinked spongy wound dressing, which comprises the following steps:
(1) Uniformly mixing polyvinyl alcohol, chitosan and gelatin according to the mass ratio of 1;
(2) And (3) sequentially adding 0.0032g of ciprofloxacin hydrochloride, 1.26g of glycerol and 0.5g of glutaraldehyde into the first component mixture, magnetically stirring for 30min, performing ultrasonic defoaming, and coating the mixed solution on a glass plate to form a film, thus obtaining the wound dressing.
Cutting the obtained membrane into a wafer with the diameter of 10mm, performing slow release in phosphate buffer solution for 0d, 7d, 14d and 21d, taking out, drying and performing an antibacterial experiment.
Comparative example 1
This comparative example differs from example 1 in that no glutaraldehyde was added to the dressing and no cross-linked network was formed.
Mixing polyvinyl alcohol, chitosan and gelatin according to a mass ratio of 1.
TABLE 1 composite film antibacterial circle diameter (mm)
FIG. 1 is a composite film cross-sectional profile, a being the cross-sectional profile of example 1 and b being the cross-sectional profile of comparative example 1.
FIG. 2 is a graph showing the antibacterial effect of the composite film, a 1 、a 2 、a 3 、a 4 The antibacterial effect of the composite membrane prepared in example 1 on staphylococcus aureus after 0d, 7d, 14d and 21d of slow release is shown in b 1 、b 2 、b 3 、b 4 Is a graph of the antibacterial effect of the composite membrane prepared in the comparative example 1 on staphylococcus aureus after 0d, 7d, 14d and 21d of slow release.
In combination with table 1, fig. 1 and fig. 2, it can be seen that a micro-crosslinked structure can be formed by adding glutaraldehyde to the composite membrane in example 1, while a crosslinked structure is not formed in comparative example 1. With the increase of the slow release time, the diameters of the inhibition zones of the composite membranes prepared in the embodiment 1 and the comparative example 1 are gradually reduced, when the slow release time reaches 21d, the diameter of the inhibition zone of the composite membrane prepared in the embodiment 1 is still 18mm, and the diameter of the inhibition zone of the composite membrane prepared in the comparative example 1 only reaches 11mm. The results show that the addition of glutaraldehyde in the composite membrane can effectively prolong the antibacterial period of the composite membrane and maintain the effect of rapid sterilization in the initial stage.
The membranes prepared in example 1 and comparative example 1 were subjected to mechanical property analysis, and each membrane was tested 5 times, and the average value was taken.
TABLE 2 comparison of mechanical properties of composite films
| Tensile strength/MPa | Elongation at break/% | |
| Example 1 | 1.06±0.22 | 95.37±0.12 |
| Comparative example 1 | 0.49±0.10 | 42.53±0.08 |
Table 2 shows that the composite film prepared in example 1 is superior to the composite film prepared in comparative example 1 in terms of tensile strength and elongation at break, which further illustrates that the mechanical properties of the composite film can be improved by adding glutaraldehyde.
Example 2
This example is essentially the same as example 1, except that ciprofloxacin hydrochloride was added in an amount of 0.0016g.
Example 3
This example is essentially the same as example 1, except that ciprofloxacin hydrochloride was added in an amount of 0.0008g.
Comparative example 2
This comparative example 2 is essentially the same as example 1, except that ciprofloxacin hydrochloride was added in an amount of 0g.
TABLE 3 diameter of the composite film antibacterial ring (mm)
Fig. 2 and 3 are diagrams of the antibacterial effect of the composite film: a is 1 、a 2 、a 3 、a 4 The antibacterial effect of the composite membrane prepared in example 1 on staphylococcus aureus after 0d, 7d, 14d and 21d of slow release is shown in c 1 、c 2 、c 3 、c 4 The antibacterial effect of the composite membrane prepared in example 2 on staphylococcus aureus after 0d, 7d, 14d and 21d of slow release is shown in d 1 、d 2 、d 3 、d 4 The antibacterial effect of the composite membrane prepared in example 3 on staphylococcus aureus after 0d, 7d, 14d and 21d of slow release is shown in e 1 Is a graph of the antibacterial effect of the composite membrane prepared in the comparative example 2 on staphylococcus aureus after 0d of slow release.
As can be seen from fig. 2, 3 and table 3, the diameters of the inhibition zones of the composite membranes prepared in example 1 (ciprofloxacin hydrochloride content of 0.02%), example 2 (ciprofloxacin hydrochloride content of 0.01%) and example 3 (ciprofloxacin hydrochloride content of 0.005%) showed a tendency of gradually decreasing with increasing time of the slow release, while the composite membrane prepared in comparative example 2 (ciprofloxacin hydrochloride content of 0%) showed no inhibition effect. The experimental result shows that the example 2 shows the best bacteriostasis in the slow release process, and particularly, the diameters of the bacteriostasis rings are 29mm and 25mm after the slow release for 7 days and 14 days respectively.
Example 5
This example provides a micro-crosslinked sponge wound dressing comprising a first component and a second component; the first component comprises: according to the parts by weight, 20 parts of polyvinyl alcohol, 45 parts of chitosan and 45 parts of gelatin; the second component comprises an antibacterial agent, glycerol and glutaraldehyde; wherein the mass of the glutaraldehyde accounts for 5 percent of the total mass of the first component; the mass of the antibacterial agent is 0.008 percent of the total mass of the first component, and the mass of the glycerol is 1 percent of the total mass of the first component.
The preparation method of this example is the same as that of example 1, and the sustained-release time and the antibacterial effect of the micro-crosslinked sponge-like wound dressing prepared by this example are similar to those of example 1.
Claims (7)
1. A micro-crosslinked sponge wound dressing comprising a first component and a second component; the first component comprises: according to the weight portion, 10 to 20 portions of polyvinyl alcohol, 30 to 45 portions of chitosan and 30 to 45 portions of gelatin; the second component comprises an antibacterial agent, glycerol and glutaraldehyde; wherein the mass of the glutaraldehyde accounts for 0.5-5% of the total mass of the first component.
2. The micro-crosslinked sponge-like wound dressing of claim 1, wherein said antimicrobial agent is ciprofloxacin hydrochloride.
3. The micro-crosslinked sponge wound dressing according to claim 2, wherein the antibacterial agent is present in an amount of 0.008 to 0.032% by mass based on the total mass of the first component.
4. The micro-crosslinked sponge wound dressing according to claim 1, wherein the glycerol is present in an amount of 1 to 2% by weight based on the total weight of the first component.
5. A method of making a micro-crosslinked sponge wound dressing according to any of claims 1-4, comprising the steps of:
(1) After the first component is uniformly mixed, sequentially adding an antibacterial agent, glycerol and glutaraldehyde, and stirring until the antibacterial agent, the glycerol and the glutaraldehyde are uniformly mixed to obtain a mixed solution;
(2) And (3) performing ultrasonic defoaming on the mixed solution, and coating the mixed solution on a glass plate to form a film to obtain the spongy wound dressing.
6. The method according to claim 5, wherein the stirring time in the step (1) is 20 to 30min.
7. The method according to claim 5, wherein in the step (1), the stirring is magnetic stirring.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106215228A (en) * | 2016-09-05 | 2016-12-14 | 东南大学 | A kind of composite sponge containing polyvinyl alcohol gelatin carboxyl chitosan and preparation method thereof |
| CN108744014A (en) * | 2018-06-14 | 2018-11-06 | 福州大学 | A kind of preparation method and products thereof with slow releasing function antiseptic dressing |
| CN112137985A (en) * | 2019-06-28 | 2020-12-29 | 南京理工大学 | Preparation method of chitosan-carried ciprofloxacin microspheres |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106215228A (en) * | 2016-09-05 | 2016-12-14 | 东南大学 | A kind of composite sponge containing polyvinyl alcohol gelatin carboxyl chitosan and preparation method thereof |
| CN108744014A (en) * | 2018-06-14 | 2018-11-06 | 福州大学 | A kind of preparation method and products thereof with slow releasing function antiseptic dressing |
| CN112137985A (en) * | 2019-06-28 | 2020-12-29 | 南京理工大学 | Preparation method of chitosan-carried ciprofloxacin microspheres |
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