CN115472481A - Cold table for cryoelectron microscope and control system thereof - Google Patents

Cold table for cryoelectron microscope and control system thereof Download PDF

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Publication number
CN115472481A
CN115472481A CN202210896224.XA CN202210896224A CN115472481A CN 115472481 A CN115472481 A CN 115472481A CN 202210896224 A CN202210896224 A CN 202210896224A CN 115472481 A CN115472481 A CN 115472481A
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CN
China
Prior art keywords
cold
bottom plate
plate
fixed connection
stage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210896224.XA
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Chinese (zh)
Inventor
刘智
李晓润
毛静妍
刘宇
方威
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VIVA BIOTECH (SHANGHAI) Ltd
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VIVA BIOTECH (SHANGHAI) Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VIVA BIOTECH (SHANGHAI) Ltd filed Critical VIVA BIOTECH (SHANGHAI) Ltd
Priority to CN202210896224.XA priority Critical patent/CN115472481A/en
Priority to PCT/CN2022/120458 priority patent/WO2024021269A1/en
Publication of CN115472481A publication Critical patent/CN115472481A/en
Pending legal-status Critical Current

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    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02Details
    • H01J37/20Means for supporting or positioning the objects or the material; Means for adjusting diaphragms or lenses associated with the support
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/2204Specimen supports therefor; Sample conveying means therefore
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26Electron or ion microscopes; Electron or ion diffraction tubes
    • H01J37/261Details
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26Electron or ion microscopes; Electron or ion diffraction tubes
    • H01J37/261Details
    • H01J37/265Controlling the tube; circuit arrangements adapted to a particular application not otherwise provided, e.g. bright-field-dark-field illumination

Abstract

The invention relates to the technical field of electron microscope equipment, and particularly discloses a cold stage for a freezing electron microscope and a control system thereof. The setting of above structure can remove the sample platform, is convenient for place or change observation material for place the material of observing and be located best state and position.

Description

Cold table for cryoelectron microscope and control system thereof
Technical Field
The invention relates to the technical field of electron microscope equipment, in particular to a cold table for a refrigeration electron microscope and a control system thereof.
Background
At present, a cryoelectron microscope is a device for realizing real-time observation of biological and high polymer materials sensitive to electron beams, wherein a cold source of the cryoelectron microscope basically comes from liquid helium or liquid nitrogen, but the liquid helium or liquid helium is exhausted after observation for a period of time, and the working time is short because the refrigerant liquid needs to be added again.
In the prior art, a cold source is provided by using a cold head so as to reach the refrigeration temperature of liquid helium or liquid nitrogen, so that the cold head is convenient to use and long in working time.
However, in the prior art, the sample stage is placed below the cryo-electron microscope body, and a part of the sample stage is shielded by the cryo-electron microscope body, so that the sample stage can be blocked when being used for placing observed substances, and the observed substances can not be conveniently placed.
Disclosure of Invention
The invention aims to provide a cooling table for a cryomicroscope and a control system thereof, and aims to solve the technical problems that in the prior art, due to the fact that a sample table is placed below a cryomicroscope body and part of the sample table is shielded by the cryomicroscope body, the placing of observed substances is obstructed, and the observation objects cannot be placed conveniently.
In order to achieve the above purpose, the cooling table for a cryoelectron microscope adopted by the invention comprises a cryoelectron microscope body and an object placing mechanism, wherein the object placing mechanism comprises a bottom plate, a flange ring, two adjusting units, a guide rail, a slide block and a sample table, the cryoelectron microscope body is fixedly connected with the bottom plate and positioned above the bottom plate, the flange ring is fixedly connected with the bottom plate and positioned below the bottom plate, the guide rail is fixedly connected with the bottom plate and positioned above the bottom plate, the two adjusting units are respectively arranged above the bottom plate, the output end of each adjusting unit respectively penetrates through the guide rail, the slide block is slidably connected with the guide rail and positioned inside the guide rail, and the sample table is fixedly connected with the slide block and respectively positioned above the slide block.
Each adjusting unit comprises an electric cylinder and an electromagnetic assembly, the electric cylinder is fixedly connected with the bottom plate and is positioned above the bottom plate, the electromagnetic assembly is fixedly connected with the output end of the electric cylinder and is respectively positioned at one end of the output end of the electric cylinder, and the electromagnetic assembly penetrates through the guide rail.
Wherein, every the electromagnetism subassembly includes the mobile jib body, circular telegram coil and urceolus, the mobile jib body with the output fixed connection of electric jar, and be located the one end of the output of electric jar, just the mobile jib body runs through the guide rail, circular telegram coil with mobile jib body fixed connection to the cover is located the exterior wall of the mobile jib body, the urceolus with mobile jib body fixed connection to the cover is located the exterior wall of the mobile jib body, just circular telegram coil is located the mobile jib body with between the urceolus.
The sample table comprises a plate body, a cold guide plate, a connecting sleeve and an outer ring, wherein the plate body is fixedly connected with the sliding block and is positioned above the sliding block, the cold guide plate is fixedly connected with the plate body and is positioned above the plate body, the connecting sleeve is fixedly connected with the cold guide plate and is positioned on the outer surface wall of the cold guide plate, and the outer ring is fixedly connected with the cold guide plate and is positioned above the cold guide plate.
The sample table further comprises cold conducting glue, and the cold conducting glue is uniformly laid on the inner surface wall of the connecting sleeve.
The cooling table for the freezing electron microscope further comprises a cold guide mechanism, and the cold guide mechanism is arranged above the bottom plate.
Wherein, lead cold mechanism includes support body, leads cold pipe, contact plate, casing and insulation material, the support body with bottom plate fixed connection, and be located the top of bottom plate, the casing with support body fixed connection, and be located the top of support body, lead cold pipe set up in the inside of support body, just lead cold pipe with fill between the support body insulation material, the contact plate with lead cold pipe fixed connection, and be located lead the one end of cold pipe.
The cold guide mechanism further comprises an inner transmission pipe, the inner transmission pipe is arranged inside the cold guide pipe, and one end of the inner transmission pipe is arranged in a round angle mode.
The invention also provides a control system which comprises a refrigeration switch, a temperature adjusting knob and a driving switch, wherein the refrigeration switch is arranged above the bottom plate, the temperature adjusting knob is arranged above the bottom plate, the driving switch is arranged above the bottom plate, and the driving switch is electrically connected with each electromagnetic coil and each electric cylinder.
According to the cold table for the cryoelectron microscope and the control system thereof, the observation sample is placed on the sample table, one of the adjusting units is started to adjust the slide block, the slide block is pushed on the guide rail until the slide block is pushed to one side of the guide rail, the other adjusting component is further started to drive the slide block to be pushed to one side of the guide rail, the sample table is positioned under the cryoelectron microscope body to be observed, the sample table is connected with the cooling end, and the sample table can be moved by the arrangement of the structure, so that the observation substance can be placed or replaced conveniently, and the placed and observed substance is positioned in the best state and position.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a first embodiment of the present invention.
Fig. 2 is a top view of a first embodiment of the present invention.
Fig. 3 is a front view of the first embodiment of the present invention.
Fig. 4 isbase:Sub>A cross-sectional view of thebase:Sub>A-base:Sub>A line structure of fig. 2 of the present invention.
Fig. 5 is an enlarged view of a portion of the structure of fig. 4B.
Fig. 6 is a front view of a second embodiment of the present invention.
Fig. 7 is a cross-sectional view of the C-C line structure of fig. 6 of the present invention.
Fig. 8 is a schematic structural diagram of a third embodiment of the present invention.
101-a cryoelectron microscope body, 102-a bottom plate, 103-a flange ring, 104-a guide rail, 105-a slide block, 106-an electric cylinder, 107-a main rod body, 108-an electrified coil, 109-an outer cylinder, 110-a plate body, 111-a cold conduction plate, 112-a connecting sleeve, 113-an outer ring, 114-a cold conduction glue, 201-a frame body, 202-a cold conduction pipe, 203-a contact plate, 204-a shell, 205-a heat insulation material, 206-an inner transmission pipe, 301-a ring frame, 302-a cold head, 303-a lantern ring, 304-a spring, 305-an adjusting ring, 306-a limit pin, 401-a refrigeration switch, 402-a temperature adjusting knob and 403-a driving switch.
Detailed Description
The first embodiment of the present application is:
referring to fig. 1 to 5, wherein fig. 1 isbase:Sub>A schematic structural view ofbase:Sub>A first embodiment, fig. 2 isbase:Sub>A top view of the first embodiment, fig. 3 isbase:Sub>A front view of the first embodiment, fig. 4 isbase:Sub>A sectional view ofbase:Sub>A linebase:Sub>A-base:Sub>A structure of fig. 2, and fig. 5 is an enlarged view ofbase:Sub>A portion ofbase:Sub>A structure B of fig. 4, the present invention providesbase:Sub>A cooling stage forbase:Sub>A cryoelectron microscope: including cryo-electron microscope body 101 and put thing mechanism, it includes bottom plate 102, flange ring 103, adjusting unit, guide rail 104, slider 105 and sample platform to put thing mechanism, adjusting unit includes electric jar 106 and electromagnetic component, electromagnetic component includes the main rod body 107, circular telegram coil 108 and urceolus 109, sample platform includes plate body 110, leads cold drawing 111, connecting sleeve 112, outer loop 113 and leads cold glue 114.
For the present embodiment, the electric cylinder 106 drives the electromagnetic assembly to move, the sliding block 105 is moved by using the cooperation between the electric cylinder 106 and the electromagnetic assembly, and the sample stage is moved, and the cold conducting plate 111 and the connecting sleeve 112 can conduct low temperature, so as to freeze an observation sample.
The cryo-electron microscope body 101 is fixedly connected with the bottom plate 102 and is located above the bottom plate 102, the flange ring 103 is fixedly connected with the bottom plate 102 and is located below the bottom plate 102, the guide rail 104 is fixedly connected with the bottom plate 102 and is located above the bottom plate 102, the number of the adjusting units is two, the two adjusting units are respectively arranged above the bottom plate 102, the output end of each adjusting unit penetrates through the guide rail 104, the slider 105 is slidably connected with the guide rail 104 and is located inside the guide rail 104, the sample stage is fixedly connected with the slider 105 and is located above the slider 105, an observation sample is placed on the sample stage, one of the adjusting units is started to adjust the slider 105, the slider 105 is pushed on the guide rail 104 until pushed to one side of the guide rail 104, the other adjusting unit is further started to drive, the slider 105 is pushed to one side of the guide rail 104, the sample stage is located right below the bottom plate 101 to perform observation, the sample stage is connected with the flange ring 103, and is used for installing the cryo-electron microscope, and the sample stage is used for installing the cryo-electron microscope.
Secondly, the electric cylinder 106 is fixedly connected with the bottom plate 102 and is located above the bottom plate 102, the electromagnetic assembly is fixedly connected with the output end of the electric cylinder 106 and is respectively located at one end of the output end of the electric cylinder 106, the electromagnetic assembly penetrates through the guide rail 104, the electromagnetic assembly can be stretched by the telescopic driving of the electric cylinder 106, and meanwhile, the sliding block 105 is electromagnetically attracted through the electromagnetic assembly, so that the purpose of moving and adjusting the sliding block 105 is achieved.
Simultaneously, the body of rod 107 with the output fixed connection of electric cylinder 106, and be located the one end of the output of electric cylinder 106, just the body of rod 107 runs through guide rail 104, circular telegram coil 108 with body of rod 107 fixed connection, and the cover is located the exterior wall of the body of rod 107, urceolus 109 with body of rod 107 fixed connection, and the cover is located the exterior wall of the body of rod 107, just circular telegram coil 108 is located the body of rod 107 with between the urceolus 109, urceolus 109 is right circular telegram coil 108 protects, is moving simultaneously during the slider 105, it is right circular telegram coil 108 is switched on, and then makes the body of rod 107 possesses the electromagnetic force, makes the body of rod 107 is right slider 105 carries out magnetism and inhales fixedly.
In addition, the plate body 110 is fixedly connected to the slider 105 and is located above the slider 105, the cold conducting plate 111 is fixedly connected to the plate body 110 and is located above the plate body 110, the connecting sleeve 112 is fixedly connected to the cold conducting plate 111 and is located on the outer surface wall of the cold conducting plate 111, the outer ring 113 is fixedly connected to the cold conducting plate 111 and is located above the cold conducting plate 111, the connecting sleeve 112 is used for connecting cold transfer equipment, the plate body 110 supports the cold conducting plate 111, the outer ring 113 presses and holds the cold conducting plate 111, and a container capable of loading an observation object is formed at the same time, wherein the cold conducting plate 111 and the connecting sleeve 112 are both made of copper or silver.
Furthermore, the cold conducting glue 114 is uniformly laid on the inner surface wall of the connecting sleeve 112, and the cold conducting glue 114 can be laid to eliminate the connecting gap when connecting cold conducting equipment, so that the conduction of low temperature is enhanced.
When the cold stage for the cryoelectron microscope of this embodiment is used, an observation sample is placed on the sample stage, one of the adjusting units is started to adjust the slide block 105, the slide block 105 is pushed on the guide rail 104 until the slide block is pushed to one side of the guide rail 104, and then the other adjusting component is started to drive, the slide block 105 is pushed to one side of the guide rail 104, so that the sample stage is located under the cryoelectron microscope body 101 to observe, and the sample stage is connected with the cooling end, in the adjusting unit, the electromagnetic component can be extended and retracted by the extension and retraction driving of the electric cylinder 106, and the slide block 105 is electromagnetically attracted by the electromagnetic component, so as to achieve the purpose of movably adjusting the slide block 105, among the electromagnetic component, urceolus 109 is right energizing coil 108 protects, is moving simultaneously during slider 105, right energizing coil 108 carries out the circular telegram, and then makes the king-post body 107 possesses the electromagnetic force, makes the king-post body 107 is right slider 105 carries out magnetism and inhales fixedly among the sample platform, connecting sleeve 112 is used for connecting the conduction cold equipment, plate body 110 is right lead cold plate 111 to support, simultaneously outer loop 113 is to lead cold plate 111 to press and hold, forms the container that can load the observation thing simultaneously, wherein lead cold plate 111 with connecting sleeve 112 all adopts copper or silver to make, the laying of cold glue 114 of leading can eliminate the joint gap when connecting the conduction cold equipment, the microthermal conduction of reinforcing.
The second embodiment of the present application is:
referring to fig. 6 and 7 based on the first embodiment, wherein fig. 6 is a front view of the second embodiment, and fig. 7 is a cross-sectional view of the structure of C-C line of fig. 6, the present invention provides a cold stage for a cryoelectron microscope: the device further comprises a cold guide mechanism, wherein the cold guide mechanism comprises a frame body 201, a cold guide pipe 202, a contact plate 203, a shell 204, a thermal insulation material 205 and an inner transfer pipe 206.
In the present embodiment, the contact plate 203 conducts low temperature, the housing 204 protects the thermal insulation material 205 and the cold conduction pipe 202, and the thermal insulation material 205 can prevent temperature loss.
Wherein, the support body 201 with bottom plate 102 fixed connection, and be located the top of bottom plate 102, the casing 204 with support body 201 fixed connection, and be located the top of support body 201, cold conduction pipe 202 set up in the inside of support body 201, just cold conduction pipe 202 with fill between the support body 201 insulation material 205, contact plate 203 with cold conduction pipe 202 fixed connection, and be located cold conduction pipe 202's one end, support body 201 is right casing 204 supports, simultaneously casing 204 is right insulation material 205 with cold conduction pipe 202 wraps up, insulation material 205 can prevent cold conduction pipe 202 reduces the loss of temperature when conducting low temperature, contact plate 203 is used for contacting the output of refrigeration plant to this reaches cold conduction's purpose.
Secondly, interior biography pipe 206 set up in the inside of leading cold tube 202, just the one end of interior biography pipe 206 is the fillet setting, interior biography pipe 206 adopts copper or silver to make for lead cold efficiency higher, adopts the fillet setting simultaneously, can be better be convenient for connecting sleeve 112 channels into.
Use a cryo-electron microscope of this embodiment during with cold stage, the support body 201 is right casing 204 supports, simultaneously casing 204 is right insulation material 205 with lead cold pipe 202 and wrap up, insulation material 205 can prevent lead cold pipe 202 and reduce the loss of temperature when conducting low temperature, contact plate 203 is used for contacting refrigeration plant's output to this reaches the purpose of leading cold, interior biography pipe 206 adopts copper or silver to make for it is more efficient to lead cold, adopts the fillet setting simultaneously, can be better be convenient for connecting sleeve 112 channels into.
The third embodiment of the present application is:
referring to fig. 8 based on the second embodiment, wherein fig. 8 is a schematic structural diagram of a third embodiment, the present invention provides a cooling stage for a cryoelectron microscope: also included is a refrigeration mechanism that includes a ring mount 301, a coldhead 302, a collar 303, a spring 304, an adjustment ring 305, and a stop pin 306.
In the present embodiment, the coldhead 302 is used to produce low temperature and conduct through the contact plate 203, and the coldhead 302 is disposed above the contact plate 203 through the ring frame 301, and the height of the collar 303 is adjusted through the adjusting ring 305, so as to adjust the height of the coldhead 302.
The ring frame 301 is fixedly connected to the housing 204 and sleeved on an outer surface wall of the housing 204, the number of the collars 303 is plural, each collar 303 is respectively fixedly connected to the cold head 302 and respectively located on the outer surface wall of the cold head 302, each collar 303 is sleeved on the outer surface wall of the ring frame 301, the number of the springs 304 is plural, each spring 304 is respectively sleeved on the outer surface wall of the ring frame 301 and respectively located below the corresponding collar 303, the number of the adjusting rings 305 is plural, each adjusting ring 305 is respectively in threaded connection with the ring frame 301 and respectively sleeved on the outer surface wall of the ring frame 301, the cold head 302 is manufactured at a low temperature and is conducted through the contact plate 203, the cold head 302 is arranged above the contact plate 203 through the ring frame 301, and the height of the collar 303 is adjusted through the adjusting rings 305, so as to adjust the height of the cold head 302, and the springs 304 can make the adjusting rings 305 be clamped more tightly so that the adjusting rings 305 are not easily loosened.
Secondly, the quantity of stop pin 306 is a plurality of, every stop pin 306 respectively with ring frame 301 threaded connection to be located respectively the top of ring frame 301, stop pin 306 can prevent adjusting ring 305 breaks away from, plays effectual guard action.
When the cooling table for the cryoelectron microscope of the embodiment is used, the cold head 302 is manufactured at a low temperature, and is conducted through the contact plate 203, the cold head 302 is arranged above the contact plate 203 through the ring frame 301, and is adjusted in height of the lantern ring 303 through the adjusting ring 305, so that the height of the cold head 302 is adjusted, meanwhile, the spring 304 can enable the adjusting ring 305 to be clamped more tightly through elastic force, so that the adjusting ring 305 is not easy to loosen, and the limit pins 306 can prevent the adjusting ring 305 from being separated, thereby playing an effective protection role.
The invention also provides a control system, which comprises a refrigeration switch 401, a temperature adjusting knob 402 and a driving switch 403, wherein the refrigeration switch 401 is arranged above the bottom plate 102, the temperature adjusting knob 402 is arranged above the bottom plate 102, the driving switch 403 is arranged above the bottom plate 102, and the driving switch 403 is electrically connected with each electromagnetic coil and the electric cylinder 106.
The refrigeration switch 401 controls the coldhead 302, and turns on and off the coldhead 302, and at the same time, the temperature adjustment knob 402 makes the coldhead 302 stable as needed, the driving switch 403 controls the two electric cylinders 106 and the electromagnetic assembly, when the driving switch 403 is pressed for the first time, the slider 105 is subjected to one stroke driving, so that the slider 105 is disposed below the cryo-electron microscope body 101, and when the driving switch 403 is pressed for the second time, the slider 105 is subjected to another stroke driving, so that the slider 105 is disposed outside the cryo-electron microscope body 101, wherein one stroke driving is a preset stroke path, so as to achieve the purpose that the driving switch 403 drives the adjustment unit.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (9)

1. A cold stage for a cryo-electron microscope comprises a cryo-electron microscope body and is characterized in that,
the device also comprises an object placing mechanism;
put thing mechanism and include bottom plate, flange ring, regulating unit, guide rail, slider and sample platform, the cryo-electron microscope body with bottom plate fixed connection, and be located the top of bottom plate, the flange ring with bottom plate fixed connection, and be located the below of bottom plate, the guide rail with bottom plate fixed connection, and be located the top of bottom plate, the quantity of regulating unit is two, two the regulating unit set up respectively in the top of bottom plate, and every the regulating unit's output runs through respectively the guide rail, the slider with guide rail sliding connection, and be located the inside of guide rail, the sample platform with slider fixed connection, and be located respectively the top of slider.
2. A cold stage for a cryoelectron microscope according to claim 1, wherein the cold stage comprises a first stage body and a second stage body,
every the regulating unit includes electric jar and electromagnetic component, the electric jar with bottom plate fixed connection, and be located the top of bottom plate, the electromagnetic component with the output fixed connection of electric jar, and be located respectively the one end of the output of electric jar, just the electromagnetic component runs through the guide rail.
3. A cold stage for a cryoelectron microscope according to claim 2,
every the electromagnetic component includes the mobile jib body, circular telegram coil and urceolus, the mobile jib body with the output fixed connection of electric jar, and be located the one end of the output of electric jar, just the mobile jib body runs through the guide rail, the circular telegram coil with mobile jib body fixed connection to the cover is located the exterior wall of the mobile jib body, the urceolus with mobile jib body fixed connection to the cover is located the exterior wall of the mobile jib body, just circular telegram coil is located the mobile jib body with between the urceolus.
4. A cold stage for a cryoelectron microscope according to claim 3,
the sample table comprises a plate body, a cold guide plate, a connecting sleeve and an outer ring, wherein the plate body is fixedly connected with the sliding block and is positioned above the sliding block, the cold guide plate is fixedly connected with the plate body and is positioned above the plate body, the connecting sleeve is fixedly connected with the cold guide plate and is positioned on the outer surface wall of the cold guide plate, and the outer ring is fixedly connected with the cold guide plate and is positioned above the cold guide plate.
5. A cold stage for a cryoelectron microscope according to claim 4 wherein,
the sample stage further comprises cold conducting glue, and the cold conducting glue is uniformly laid on the inner surface wall of the connecting sleeve.
6. A cold stage for a cryoelectron microscope according to claim 5 wherein,
the cold table for the cryoelectron microscope further comprises a cold guide mechanism, and the cold guide mechanism is arranged above the bottom plate.
7. A cold stage for a cryoelectron microscope according to claim 6,
lead cold mechanism and include support body, lead cold pipe, contact plate, casing and insulation material, the support body with bottom plate fixed connection, and be located the top of bottom plate, the casing with support body fixed connection, and be located the top of support body, lead cold pipe set up in the inside of support body, just lead cold pipe with fill between the support body insulation material, the contact plate with lead cold pipe fixed connection, and be located lead the one end of cold pipe.
8. A cold stage for a cryoelectron microscope according to claim 7,
the cold guide mechanism further comprises an inner transmission pipe, the inner transmission pipe is arranged inside the cold guide pipe, and one end of the inner transmission pipe is arranged in a round angle mode.
9. A control system for controlling a cryo-electron microscope stage according to claim 8,
including refrigeration switch, temperature regulation knob and drive switch, the refrigeration switch set up in the top of bottom plate, temperature regulation knob set up in the top of bottom plate, drive switch set up in the top of bottom plate, just drive switch and every solenoid with electric jar electric connection.
CN202210896224.XA 2022-07-28 2022-07-28 Cold table for cryoelectron microscope and control system thereof Pending CN115472481A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202210896224.XA CN115472481A (en) 2022-07-28 2022-07-28 Cold table for cryoelectron microscope and control system thereof
PCT/CN2022/120458 WO2024021269A1 (en) 2022-07-28 2022-09-22 Cryo-stage for cryo-electron microscope and control system therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210896224.XA CN115472481A (en) 2022-07-28 2022-07-28 Cold table for cryoelectron microscope and control system thereof

Publications (1)

Publication Number Publication Date
CN115472481A true CN115472481A (en) 2022-12-13

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Application Number Title Priority Date Filing Date
CN202210896224.XA Pending CN115472481A (en) 2022-07-28 2022-07-28 Cold table for cryoelectron microscope and control system thereof

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WO (1) WO2024021269A1 (en)

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Publication number Priority date Publication date Assignee Title
JP2007080698A (en) * 2005-09-15 2007-03-29 Fuji Electric Holdings Co Ltd Sample analyzer
CN109632450B (en) * 2018-11-19 2020-09-25 浙江大学 Mechanism for cooling and transmitting sample in sealed cavity
CN110299275B (en) * 2019-07-03 2021-07-30 业成科技(成都)有限公司 Transmission rod and scanning electron microscope
CN212622160U (en) * 2020-05-14 2021-02-26 西北农林科技大学 Sample room, temperature-adjusting carrying device, microscope temperature-controlling observation platform and system
CN214252099U (en) * 2020-12-31 2021-09-21 厦门超新芯科技有限公司 Frozen sample platform of scanning electron microscope
US11728146B2 (en) * 2021-01-13 2023-08-15 Wisconsin Alumni Research Foundation Retractable ion guide, grid holder, and technology for removal of cryogenic sample from vacuum
CN214848485U (en) * 2021-06-18 2021-11-23 深圳市福田区南科大量子技术与工程研究院 Cold table for cryoelectron microscope

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