CN115463588A - 3D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell - Google Patents
3D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell Download PDFInfo
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- CN115463588A CN115463588A CN202211020463.5A CN202211020463A CN115463588A CN 115463588 A CN115463588 A CN 115463588A CN 202211020463 A CN202211020463 A CN 202211020463A CN 115463588 A CN115463588 A CN 115463588A
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 50
- 230000029087 digestion Effects 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 238000010438 heat treatment Methods 0.000 claims abstract description 16
- 238000005192 partition Methods 0.000 claims description 13
- 239000004973 liquid crystal related substance Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 238000003860 storage Methods 0.000 abstract description 5
- 238000000151 deposition Methods 0.000 abstract description 4
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- 210000001519 tissue Anatomy 0.000 description 23
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
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- 210000003958 hematopoietic stem cell Anatomy 0.000 description 2
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- 206010067482 No adverse event Diseases 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
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- 238000005299 abrasion Methods 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
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- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/90—Heating or cooling systems
- B01F35/92—Heating or cooling systems for heating the outside of the receptacle, e.g. heated jackets or burners
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- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
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Abstract
The embodiment of the application provides a 3D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell, relates to thermostated container technical field. Comprises an outer box body, a heating plate, a condensing plate, a rotating assembly and a positioning assembly; the heating plates and the condensing plates are respectively arranged in two groups, the heating plates are respectively arranged on the upper side and the lower side of the inner wall of the outer box body, and the condensing plates are respectively arranged on the left side and the right side of the inner wall of the outer box body. According to the 3D rotation type thermostated container that is applicable to multiple tissue digestion preparation mesenchymal stem cell of this application, can carry out the reagent bottle of depositing mesenchymal stem cell omnidirectional abundant rotation and rock, eliminate the possibility of layering under the effect of gravity when the mesenchymal stem cell is in the storage to adjust corresponding temperature as required, put into corresponding mesenchymal stem cell reagent bottle quantity as required, and can make the mesenchymal stem cell in each reagent bottle all can obtain even being heated and even rocking.
Description
Technical Field
The application relates to the technical field of thermostats, particularly, relate to 3D rotation type thermostats suitable for mesenchymal stem cell of multiple tissue digestion preparation.
Background
Animal tissue pieces are generally treated with pancreatin prior to culturing animal cells to digest the intercellular junction proteins (extracellular matrix) of the animal tissue pieces and disperse the tissue pieces into individual cells, which is the digestion of animal tissue.
In this tissue digestion preparation, it is an important biological experimental part in the fabrication of mesenchymal stem cells, which are pluripotent stem cells having all the commonalities of stem cells, i.e., self-renewal and multipotent differentiation ability. The application is most in clinical application, and the combined application with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstruction. After a patient receives a large dose of chemotherapy, the mesenchymal stem cells and the hematopoietic stem cells are input together, so that the recovery time of the blood cells of the patient can be obviously accelerated, the safety is realized, no adverse reaction is caused, and the mesenchymal stem cells exist in bone marrow, skeletal muscle, periosteum and trabecula. It has wide tissue types, so it is of great clinical application value.
But present mesenchymal stem cell need get into the thermostated container and preserve after preliminary preparation is accomplished, but in order not to let mesenchymal stem cell in the preservation because layering under the effect of gravity, but inside thermostated container that possess revolution mechanic can be circular motion on same water flat line only, the effect is not very good, and can make the mesenchymal stem cell of preserving in the thermostated container cause the influence when the vapor in extra ponding or the air gets into the thermostated container, and when needs rotatory preservation stem cell, the rotation and the operation of 1-3 medicament bottles can only be held to general rotating equipment once, the efficiency is lower, consequently, it solves above-mentioned problem to provide the 3D rotation type thermostated container that is applicable to various tissue digestions preparation mesenchymal stem cell.
Disclosure of Invention
The present application is directed to solving at least one of the problems in the prior art. For this reason, this application provides a D rotation type thermostated container suitable for mesenchymal stem cell of multiple tissue digestion preparation, can a plurality of mesenchymal stem cells of D multi-angle rotating, and storage effect is better.
According to the D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell of this application embodiment, a D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell includes: the device comprises an outer box body, a heating plate, a condensing plate, a rotating assembly and a positioning assembly;
two groups of heating plates and two groups of condensing plates are arranged, the two groups of heating plates are respectively arranged on the upper side and the lower side of the inner wall of the outer box body, and the two groups of condensing plates are respectively arranged on the left side and the right side of the inner wall of the outer box body;
the rotating assembly is arranged on the left side of the inner wall of the outer box body, and one end of the rotating assembly, which is far away from the inner wall of the outer box body, is rotatably connected with the positioning assembly;
wherein, the front of outer box articulates there is sealing door.
According to the D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell of this application embodiment, the internally mounted of outer container has porous partition, porous partition's bottom is provided with the collection box that is used for catching porous partition hourglass water, collect box and outer internal wall sliding connection, and the front of collecting the box extends to the front of outer container.
According to the D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell of this application embodiment, the back mounted of outer internal wall of box has two suction fans and two exhaust fans.
According to the D rotary type incubator suitable for preparing the mesenchymal stem cells through digestion of various tissues, the rotary component comprises a first servo motor, a first rotary rod, a second servo motor, a positioning block and a rotary block;
one end of the first rotating rod is connected with an output shaft of the first servo motor through a coupler, the other end of the first rotating rod is fixedly connected with the positioning block, the positioning block is rotatably connected with the rotating block, and the rotating block is fixedly connected with the positioning assembly through a screw.
According to the D rotary type incubator suitable for preparing the mesenchymal stem cells through digestion of various tissues, the rotating assembly further comprises a second servo motor, a driving fluted disc and a driven fluted disc;
the second servo motor is installed on first pivot pole, and the output shaft of second servo motor passes through the shaft coupling and links to each other with the initiative fluted disc, the initiative fluted disc meshes with the driven fluted disc mutually, and the rear end of driven fluted disc runs through turning block, locating piece in proper order, driven fluted disc and turning block fixed connection, and driven fluted disc and locating piece rotation connection.
According to the D rotary type incubator suitable for preparing the mesenchymal stem cells through digestion of various tissues, the positioning assembly comprises a positioning plate, a first rubber positioning block, a second rubber positioning block and a sliding block;
the number of the first rubber positioning blocks is multiple, the bottoms of the first rubber positioning blocks are fixedly connected with the top of the positioning plate, the number of the second rubber positioning blocks is multiple, the bottoms of the second rubber positioning blocks are fixedly connected with the adjacent sliding blocks, and expansion springs are fixedly connected between the adjacent two sliding blocks; and the one end of the adjacent sliding block of leftmost expanding spring principle and the inner wall fixed connection of locating plate, it is a plurality of the sliding block all with the inner wall sliding connection of locating plate.
According to the D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell of this application embodiment, the bottom fixed mounting of locating plate has electric telescopic handle, electric telescopic handle's output pole and connecting plate fixed connection, and the top of connecting plate with adjacent second rubber locating piece fixed connection.
According to the D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell of this application embodiment, the magnetic attraction board is installed to the bottom of locating plate, the bottom adsorption of magnetic attraction board has two counter weight magnetic paths.
According to the D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell of this application embodiment, the camera is all installed with the left and right sides at porous partition top in the top of outer box inner wall, and the right side of outer box installs liquid crystal display, and is a plurality of the signal output part of camera all with liquid crystal display's video signal receiving terminal electric connection.
According to the D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell of this application embodiment, a plurality of location strips are all installed to outer box inner wall's the left and right sides, and equal block has the thing board of putting between two location strips on the same water flat line.
The invention has the beneficial effects that:
1. the device wholly adopts outer box as the main part of depositing the mesenchymal stem cell, and the inside of box is provided with the board cooling of heating, the fridge intensifies outside to two sets of distributions that heat board, fridge respectively in the top of outer box inner wall and the left and right sides of outer box inner wall guarantee when the temperature regulation, wrap up locating component in the intermediate position all the time, guarantee that locating component is heated/cold even.
2. The device adopts camera and liquid crystal display to cooperate, can let the operator follow the outside and can observe operation condition such as outer box internal portion locating component, rotating assembly and heating plate, condensing panel, can provide emergency treatment when the bottle on the locating component drops or spills.
3. The device is provided with rotating assembly, can be different with the tradition only horizontal rotation, and in the inside of outer box, can utilize rotating assembly to drive clockwise or anticlockwise rotation in the locating component vertical direction, can also rotate along the horizontal direction slope, form the position rotation of an omnidirectional.
4. The device is on locating component, and a plurality of fixed first rubber locating piece that utilize on the locating plate cooperate with gliding second rubber locating piece, and the sliding block drives the motion of second rubber locating piece, and second rubber locating piece can utilize the cooperation sliding block that contracts of expanding spring's the back to be close to adjacent first rubber locating piece, with mesenchymal stem cell centre gripping after, can also utilize electric telescopic handle, connecting plate further to consolidate, cooperation magnetic suction plate, counter weight magnetic path counter weight.
5. The device is not the central position of the positioning component at the connecting part of the rotating component and the positioning component, but is the position which is on the upper side of the middle of the positioning component, so that the rotating area and the corresponding centrifugal force can be increased when the device rotates, and the rotation is more uniform.
6. Whole device utilizes the location strip, puts the thing board and can change the inside usage of outer box, is about to locating component take off and puts into can put into other things that need the constant temperature to preserve behind the corresponding thing board of putting.
To summarize: whole device can carry out the reagent bottle of depositing the mesenchymal stem cell omnidirectional abundant rotation and rock, eliminate the mesenchymal stem cell because the possibility of layering under the effect of gravity when the storage, and adjust the temperature that corresponds as required, put into corresponding mesenchymal stem cell reagent bottle quantity as required, and can make the mesenchymal stem cell in each reagent bottle all can obtain even being heated and even rocking, and can also switch the effect of outer box as required, also can realize quick discharge when ponding, can not pile up the inside at outer box.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments of the present application will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and that those skilled in the art can also obtain other related drawings based on the drawings without inventive efforts.
FIG. 1 is a schematic perspective view of a first perspective view according to an embodiment of the present disclosure;
FIG. 2 is a schematic perspective view of a second perspective view according to an embodiment of the present disclosure;
FIG. 3 is an elevational cross-sectional view according to an embodiment of the present application;
FIG. 4 is a partial sectional elevation view of a rotating assembly and a positioning assembly coupled together according to an embodiment of the present application;
FIG. 5 is an elevational, cross-sectional view of a positioning assembly according to an embodiment of the present application;
FIG. 6 is an enlarged view at A of FIG. 3 according to an embodiment of the present application;
FIG. 7 is a perspective view of a positioning assembly according to an embodiment of the present application.
An icon:
1. an outer case; 11. a sealing door; 12. heating the plate; 13. a camera; 14. a condensing plate; 15. A liquid crystal display screen; 16. a suction fan; 17. an exhaust fan; 2. a rotating assembly; 21. a first servo motor; 22. a first rotating lever; 23. a second servo motor; 24. a driving fluted disc; 25. a driven fluted disc; 26. rotating the block; 27. positioning blocks; 3. a positioning assembly; 31. positioning a plate; 32. a first rubber positioning block; 33. a second rubber positioning block; 34. a slider; 35. a tension spring; 36. an electric telescopic rod; 37. a connecting plate; 38. a magnetic attraction plate; 39. a counterweight magnetic block; 4. a positioning bar; 5. a placement plate; 6. A porous separator; 7. and (4) collecting the box.
Detailed Description
The technical solutions in the embodiments of the present application will be described below with reference to the drawings in the embodiments of the present application.
To make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making creative efforts shall fall within the protection scope of the present application.
In the case of the example 1, the following examples are given,
a 3D rotary incubator suitable for the preparation of mesenchymal stem cells by digestion of various tissues according to an embodiment of the present application will be described below with reference to the accompanying drawings.
As shown in fig. 1 to 7, a 3D rotary incubator suitable for preparing mesenchymal stem cells through digestion of various tissues according to an embodiment of the present application includes: the device comprises an outer box body 1, a heating plate 12, a condensing plate 14, a rotating assembly 2 and a positioning assembly 3;
two groups of heating plates 12 and two groups of condensing plates 14 are arranged, wherein the two groups of heating plates 12 are respectively arranged on the upper side and the lower side of the inner wall of the outer box 1, and the two groups of condensing plates 14 are respectively arranged on the left side and the right side of the inner wall of the outer box 1;
the rotating component 2 is arranged on the left side of the inner wall of the outer box body 1, and one end, far away from the inner wall of the outer box body 1, of the rotating component 2 is rotatably connected with the positioning component 3;
wherein, the front of the outer box body 1 is hinged with a sealing door 11.
The device wholly adopts outer box 1 as the main part of depositing the mesenchymal stem cell, is provided with the board 12 cooling that heats, the fridge 14 intensifies in the inside of outer box 1 to the board 12 that heats, the fridge 14 two sets of distribute respectively in the top of outer box 1 inner wall and the left and right sides of outer box 1 inner wall, guarantee when the regulation temperature, wrap up locating component 3 in the intermediate position all the time, guarantee that locating component 3 is heated/cold even.
According to some embodiments of the present application, the internal installation of the outer box 1 has a porous partition 6, the bottom of the porous partition 6 is provided with a collection box 7 for catching water leakage from the porous partition 6, the collection box 7 is connected with the inner wall of the outer box 1 in a sliding manner, and the front of the collection box 7 extends to the front of the outer box 1.
According to some embodiments of the present application, two suction fans 16 and two exhaust fans 17 are installed on the back surface of the inner wall of the outer case 1.
According to some embodiments of the present application, the rotating assembly 2 includes a first servo motor 21, a first rotating rod 22, a second servo motor 23, a positioning block 27 and a rotating block 26;
one end of the first rotating rod 22 is connected with an output shaft of the first servo motor 21 through a coupler, the other end of the first rotating rod 22 is fixedly connected with a positioning block 27, the positioning block 27 is rotatably connected with a rotating block 26, and the rotating block 26 is fixedly connected with the positioning component 3 through screws.
According to some embodiments of the present application, the rotating assembly 2 further comprises a second servo motor 23, a driving toothed disc 24 and a driven toothed disc 25;
the second servo motor 23 is mounted on the first rotating rod 22, an output shaft of the second servo motor 23 is connected with the driving fluted disc 24 through a coupler, the driving fluted disc 24 is meshed with the driven fluted disc 25, the rear end of the driven fluted disc 25 sequentially penetrates through the rotating block 26 and the positioning block 27, the driven fluted disc 25 is fixedly connected with the rotating block 26, and the driven fluted disc 25 is rotatably connected with the positioning block 27.
The device is provided with rotating assembly 2, can be different with the tradition only horizontal rotation, and in the inside of outer box 1, can utilize rotating assembly 2 to drive clockwise or anticlockwise rotation on the 3 vertical direction of locating component, can also rotate along the horizontal direction slope, form the position rotation of an omnidirectional.
According to some embodiments of the present application, the positioning assembly 3 includes a positioning plate 31, a first rubber positioning block 32, a second rubber positioning block 33, and a sliding block 34;
a plurality of first rubber positioning blocks 32 are provided, the bottoms of the first rubber positioning blocks 32 are fixedly connected with the top of the positioning plate 31, a plurality of second rubber positioning blocks 33 are provided, the bottoms of the second rubber positioning blocks 33 are fixedly connected with adjacent sliding blocks 34, and expansion springs 35 are fixedly connected between the adjacent sliding blocks 34; and one end of the slide block 34 adjacent to the leftmost expansion spring 35 is fixedly connected with the inner wall of the positioning plate 31, and the slide blocks 34 are all connected with the inner wall of the positioning plate 31 in a sliding manner.
According to some embodiments of the present application, an electric telescopic rod 36 is fixedly mounted at the bottom of the positioning plate 31, an output rod of the electric telescopic rod 36 is fixedly connected with a connecting plate 37, and the top end of the connecting plate 37 is fixedly connected with the adjacent second rubber positioning block 33.
According to some embodiments of the present application, a magnetic attraction plate 38 is installed at the bottom of the positioning plate 31, and two counterweight magnetic blocks 39 are attracted to the bottom of the magnetic attraction plate 38.
The device is on locating component 3, and a plurality of fixed first rubber locating blocks 32 and gliding second rubber locating block 33 that utilize on the locating plate 31 cooperate, and sliding block 34 drives second rubber locating block 33 motion, and second rubber locating block 33 can utilize the cooperation sliding block 34 that contracts of retraction spring 35 to be close to adjacent first rubber locating block 32, after the mesenchymal stem cell centre gripping, can also utilize electric telescopic handle 36, connecting plate 37 to further consolidate, cooperate magnetic attraction board 38, counter weight magnetic path 39 counter weight.
According to some embodiments of this application, camera 13 is all installed at the top of outer box 1 inner wall and the left and right sides at 6 tops of porous partition, and liquid crystal display 15 is installed on the right side of outer box 1, a plurality of cameras 13's signal output part all with liquid crystal display 15's video signal receiving terminal electric connection.
The device adopts camera 13 and liquid crystal display 15 to cooperate, can let the operator from the outside can observe the inside locating component 3 of outer box 1, rotating assembly 2 and heat the operation condition such as board 12, condensation plate 14, can provide emergency treatment when the bottle on locating component 3 drops or leaks.
According to some embodiments of this application, a plurality of location strips 4 are all installed to the left and right sides of outer box 1 inner wall, and all the block has thing board 5 between two location strips 4 on same water flat line.
Further, embodiment 2 changes camera 13 into the camera that can respond to the temperature or adds temperature-sensing ware in the inside of outer box 1, can know the numerical value of outer box 1 inside temperature through liquid crystal display 15 in real time.
Further, in embodiment 3, the air inlets of the suction fan 16 and the exhaust fan 17 are provided with a filter screen and a valve, when the interior of the outer box 1 needs to be in a closed state, the valve is closed, and when air flow is needed, the filter screen can filter air to a certain extent, so that the possibility of dust entering the interior of the outer box 1 is reduced.
The hot plate has been added and can be dried when the grape vine material loading, and 2 cooperations of cubical switchboard have a plurality of through-holes, can be with semi-manufactured goods based on the compound science and technology board of grape vine when the cutting perhaps polishes the drawer in sucking the wardrobe 2 with the piece when linking to each other with the air suction pump, pull open the drawer and can pour the dust, improve the convenience that the device cleared up.
The working principle of the invention; when the device works, the sealing door 11 on the front side of the outer box body 1 is opened, the positioning plate 31 is inclined, after the inclination, a required number of bottles are placed into the corresponding first rubber positioning block 32 and the second rubber positioning block 33, because the sliding block 34 is restrained by the resilience force of the expansion spring 35, the bottles can be buckled in the corresponding groove, when all the bottles are buckled on the positioning plate 31, the electric telescopic rod 36 is started to drive the connecting plate 37 to retract, at the moment, the first sliding block 34 retracts, other sliding blocks 34 and expansion springs 35 linked with the first sliding block 34 correspondingly bear partial pressure, so that the adjacent first rubber positioning block 32 and second rubber positioning block 33 are close to each other to extrude the bottles, the connection between the bottles and the positioning plate 31 is more stable, the magnetic suction plate 38 and the corresponding counterweight magnetic block 39 are installed at the bottom of the positioning plate 31, the eccentrically connected positioning component 3 is correspondingly increased in counterweight, the whole electric telescopic rod 36, the positioning plate 31 and the magnetic suction plate 38 form certain balance, and the abrasion of the positioning plate 31 when the second servo motor 23 drives the positioning plate 31 is reduced.
Start first servo motor 21, drive first rotation pole 22 and rotate, later through driving fluted disc 24, driven fluted disc 25, turning block 26 drives locating plate 31 and rotates, can use the tie point of first rotation pole 22 and locating plate 31 to rotate as the axle center positioning assembly 3 this moment, and when second servo motor 23 rotated, can drive locating plate 31 and use turning block 26, the turning point of locating piece 27 and driven fluted disc 25 clockwise or anticlockwise rotation as the axle center through driving fluted disc 24, driven fluted disc 25 and turning block 26, the two cooperation can let locating assembly 3 can form the 3d formula rotation of being close to three hundred sixty degrees.
At locating component 3 pivoted in-process, according to ambient temperature, when internal temperature is less than the temperature that the storage of mesenchymal stem cell needs, both sides heat board 12 starts about this moment, rises outer 1 internal temperature of box and all right to suitable temperature, when internal temperature is higher than the temperature that the storage of mesenchymal stem cell needs, two heat board 12 refrigerates about the start, reduces outer 1 internal temperature of box and all right to suitable temperature.
When the temperature of the heating plate 12 and the condensing plate 14 is adjusted, the air is supplied by the suction fan 16 and discharged by the exhaust fan 17 to form air flow inside the outer box 1, all the operations can be transmitted to the liquid crystal display 15 through the camera 13, and the condition inside the outer box 1 can be observed by the liquid crystal display 15.
When other heat retaining article of needs are placed to needs in outer box 1 inside, cancel the connection structure between turning block 26 and the locating component 3 this moment, and take off locating component 3 after, insert on the location strip 4 that corresponds and put thing board 5, can put article on putting thing board 5 afterwards, and when article have water or when locating component 3 when the operation surface has ponding, water can fall to collect in collecting box 7 through the through-hole on the porous partition 6 and can not gather the environment in outer box 1 inner wall's bottom influence outer box 1.
The electronic components used in the invention can be selected to be proper in size and type according to actual use requirements, and are specifically selected according to actual production, use, expenses and the like.
The above embodiments are merely examples of the present application and are not intended to limit the scope of the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined or explained in subsequent figures.
The above description is only for the specific embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present application, and shall be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (10)
1. 3D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell, its characterized in that includes: the device comprises an outer box body (1), a heating plate (12), a condensing plate (14), a rotating assembly (2) and a positioning assembly (3);
two groups of heating plates (12) and two groups of condensing plates (14) are arranged, the two groups of heating plates (12) are respectively arranged on the upper side and the lower side of the inner wall of the outer box body (1), and the two groups of condensing plates (14) are respectively arranged on the left side and the right side of the inner wall of the outer box body (1);
the rotating assembly (2) is arranged on the left side of the inner wall of the outer box body (1), and one end, far away from the inner wall of the outer box body (1), of the rotating assembly (2) is rotatably connected with the positioning assembly (3);
wherein, the front surface of the outer box body (1) is hinged with a sealing door (11).
2. The 3D rotary incubator suitable for various tissue digestions to prepare mesenchymal stem cells according to claim 1, wherein a porous partition (6) is installed inside the outer box (1), a collecting box (7) for collecting water leakage from the porous partition (6) is arranged at the bottom of the porous partition (6), the collecting box (7) is slidably connected with the inner wall of the outer box (1), and the front surface of the collecting box (7) extends to the front surface of the outer box (1).
3. The 3D rotary type incubator suitable for multiple tissue digestion preparation mesenchymal stem cells according to claim 1, characterized in that two suction fans (16) and two exhaust fans (17) are installed on the back of the inner wall of the outer box body (1).
4. The 3D rotary incubator suitable for various tissue digestions to prepare mesenchymal stem cells according to claim 1, wherein the rotating assembly (2) comprises a first servo motor (21), a first rotating rod (22), a second servo motor (23), a positioning block (27) and a rotating block (26);
the one end of first pivot pole (22) is passed through the shaft coupling and is linked to each other with the output shaft of first servo motor (21), the other end and locating piece (27) fixed connection of first pivot pole (22), locating piece (27) are rotated with turning block (26) and are connected, turning block (26) pass through screw fixed connection with locating component (3).
5. The 3D rotary incubator according to claim 4, wherein the rotary assembly (2) further comprises a second servo motor (23), a driving toothed disc (24) and a driven toothed disc (25);
second servo motor (23) are installed on first pivot pole (22), and the output shaft of second servo motor (23) passes through the shaft coupling and links to each other with initiative fluted disc (24), initiative fluted disc (24) mesh mutually with driven fluted disc (25), and the rear end of driven fluted disc (25) runs through turning block (26), locating piece (27) in proper order, driven fluted disc (25) and turning block (26) fixed connection, and driven fluted disc (25) rotate with locating piece (27) and are connected.
6. The 3D rotary incubator suitable for multiple tissue digestion preparation of mesenchymal stem cells according to claim 1, wherein the positioning assembly (3) comprises a positioning plate (31), a first rubber positioning block (32), a second rubber positioning block (33), a sliding block (34);
the number of the first rubber positioning blocks (32) is multiple, the bottoms of the first rubber positioning blocks (32) are fixedly connected with the top of the positioning plate (31), the number of the second rubber positioning blocks (33) is multiple, the bottoms of the second rubber positioning blocks (33) are fixedly connected with adjacent sliding blocks (34), and telescopic springs (35) are fixedly connected between every two adjacent sliding blocks (34); and one end of the telescopic spring (35) at the leftmost side adjacent to the sliding blocks (34) is fixedly connected with the inner wall of the positioning plate (31), and the sliding blocks (34) are all in sliding connection with the inner wall of the positioning plate (31).
7. The 3D rotary incubator suitable for various tissue digestions to prepare mesenchymal stem cells according to claim 6, wherein an electric telescopic rod (36) is fixedly installed at the bottom of the positioning plate (31), an output rod of the electric telescopic rod (36) is fixedly connected with a connecting plate (37), and the top end of the connecting plate (37) is fixedly connected with the adjacent second rubber positioning block (33).
8. The 3D rotary incubator suitable for various tissue digestions to prepare mesenchymal stem cells according to claim 7, wherein a magnetic attraction plate (38) is installed at the bottom of the positioning plate (31), and two counterweight magnetic blocks (39) are attracted to the bottom of the magnetic attraction plate (38).
9. The 3D rotary incubator suitable for the preparation of mesenchymal stem cells through the digestion of various tissues according to any one of claim 1, wherein cameras (13) are installed on the top of the inner wall of the outer case (1) and the left and right sides of the top of the porous partition plate (6), a liquid crystal display (15) is installed on the right side of the outer case (1), and the signal output ends of the cameras (13) are electrically connected with the video signal receiving end of the liquid crystal display (15).
10. The 3D rotary incubator suitable for various tissue digestions to prepare mesenchymal stem cells according to any one of claim 1, wherein a plurality of positioning strips (4) are installed on both the left and right sides of the inner wall of the outer box (1), and an object placing plate (5) is clamped between two positioning strips (4) on the same horizontal line.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211020463.5A CN115463588A (en) | 2022-08-24 | 2022-08-24 | 3D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211020463.5A CN115463588A (en) | 2022-08-24 | 2022-08-24 | 3D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell |
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| CN115463588A true CN115463588A (en) | 2022-12-13 |
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| CN202211020463.5A Pending CN115463588A (en) | 2022-08-24 | 2022-08-24 | 3D rotation type thermostated container suitable for multiple tissue digestion preparation mesenchymal stem cell |
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