CN115463151B - Nano-enzyme, preparation method and application thereof, and bacteriostat - Google Patents
Nano-enzyme, preparation method and application thereof, and bacteriostat Download PDFInfo
- Publication number
- CN115463151B CN115463151B CN202211002839.XA CN202211002839A CN115463151B CN 115463151 B CN115463151 B CN 115463151B CN 202211002839 A CN202211002839 A CN 202211002839A CN 115463151 B CN115463151 B CN 115463151B
- Authority
- CN
- China
- Prior art keywords
- enzyme
- nano
- solution
- precipitate
- centrifuging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Nanotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Organic Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Communicable Diseases (AREA)
- Dermatology (AREA)
- Physics & Mathematics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Manufacturing & Machinery (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a nano-enzyme, a preparation method and application thereof, and a bacteriostatic agent, wherein the nano-enzyme is in a cube structure, and C, zn and Co are uniformly dispersed in the cube structure. The invention aims to provide a nano enzyme bacteriostatic agent with good bacteriostatic performance and good wound healing promoting effect.
Description
Technical Field
The invention relates to the technical field of biological and medical sterilization, in particular to nano-enzyme, a preparation method and application thereof, and a bacteriostatic agent.
Background
Nano enzymes are a class of nano materials with enzyme catalytic activity, and are mainly divided into two classes: one is to modify natural enzyme or groups with enzyme catalytic activity on the nanomaterial, the nanomaterial plays a role of a carrier, and the other is that the nanomaterial itself has similar catalytic activity of the enzyme. Compared with natural enzymes, the nano-enzyme has the characteristics of low price, simple preparation process, good stability, high recycling efficiency and the like, and has application prospects in the fields of detection, sewage treatment, sterilization, inflammation diminishing, cancer treatment and the like.
Bacterial diseases have become one of the biggest health problems worldwide, with thousands of people being plagued each year. The traditional antibacterial agent is mainly antibiotic, and in addition, some metal inorganic salt inorganic reagent and organic reagent such as vanillin, quaternary ammonium salt and the like can be used as antibacterial material. However, these organic and inorganic antibacterial agents often have certain drawbacks, such as complex preparation and synthesis process, high cost, easy generation of drug resistance, pollution to the surrounding environment, and the like. For this reason, new materials are increasingly being explored for antimicrobial treatment.
The repair and regeneration process of wound tissue is one of the most complex biological processes, which mainly involves cell proliferation, angiogenesis, and tissue remodeling. And the repair process may be delayed due to bacterial infection and slow differentiation of fibroblasts, resulting in local pain, swelling, even life-threatening infection complications. Thus, there is an urgent need to explore methods that can accelerate tissue repair at the wound site while performing antimicrobial therapy.
Reactive Oxygen Species (ROS) are oxygen-containing highly reactive chemicals having unpaired electrons including superoxide anions (O2) .- ) Radical of hydroxy radical . OH)、H 2 O 2 And the like, ROS can cause damage to microbial DNA, proteins, and other chemicals, ultimately leading to cell death. The nano-enzyme can regulate ROS level and is used for developing novel antibacterial medicines. MSN-AuNPs can generate excessive ROS, thereby generating powerful antibacterial properties. Has obvious inhibiting effect on proliferation of gram-positive staphylococcus aureus and gram-negative escherichia coli. The nano enzyme can be used as a mimic of peroxidase and can be used for inhibiting wound infection caused by bacteria and promoting wound healing. For example, both the magnetic nanoparticles and the graphene quantum dots have peroxide mimic enzyme activity and thus have excellent antibacterial properties in wound infection. At present, bacterial drug resistance has become a worldwide problem, and nano-enzyme can be developed into a novel antibacterial agent, so that a novel thought is provided for solving the problem.
Disclosure of Invention
The invention mainly aims to provide a nano-enzyme, a preparation method and application thereof and a bacteriostatic agent, and aims to provide the nano-enzyme bacteriostatic agent with good bacteriostatic performance and good wound healing promoting effect.
To achieve the above object, the present invention provides a nanoenzyme having a cubic structure in which C, zn and Co are uniformly dispersed.
The invention further provides a preparation method of the nano-enzyme, which comprises the following steps:
adding zinc salt and 2-methylimidazole into water, mixing and stirring uniformly, and then carrying out centrifugal treatment to obtain a precipitate;
adding cobalt salt into methanol solution, performing ultrasonic treatment, adding the precipitate, and stirring to obtain a first solution;
adding 2-methylimidazole into a methanol solution, and performing ultrasonic treatment to obtain a second solution;
and adding the second solution into the first solution, standing, centrifuging, drying and calcining to obtain the nano enzyme.
Optionally, adding zinc salt and 2-methylimidazole into water, mixing and stirring uniformly, and then performing centrifugal treatment, wherein the parameters of the centrifugal treatment are as follows:
centrifuging for 10 min-15 min at the rotation speed of 9000 rpm-10000 rpm.
Optionally, adding the second solution into the first solution, standing and centrifuging, and drying and calcining the precipitate to obtain the nano enzyme, wherein the drying and calcining comprises the steps of:
drying at 70-75 ℃ for 8-12 h, and calcining at 400-410 ℃ for 2-3 h at a heating rate of 2-3 ℃/min.
Alternatively, zinc salt and 2-methylimidazole are added into water, mixed and stirred uniformly, and then centrifuged, and the precipitate is washed with deionized water in the step of taking the precipitate.
Optionally, the method is characterized in that the second solution is added into the first solution, and the steps of standing, centrifuging, drying and calcining are carried out to obtain the nano enzyme, wherein the step of standing, centrifuging and washing with methanol are carried out.
Optionally, adding the second solution into the first solution, standing, centrifuging, drying and calcining the precipitate to obtain the nano enzyme, wherein the standing time is 8-24 h.
The invention also provides a bacteriostatic agent comprising nano-enzyme or pharmaceutically acceptable salt thereof.
Optionally, the bacteriostatic agent further comprises a compound solvent of the nano-enzyme, and the concentration of the nano-enzyme in the bacteriostatic agent is 1 mg/mL-3 mg/mL.
The invention also provides application of the nano-enzyme or the salt thereof in preparing a bacteriostatic agent.
The nano-enzyme provided by the invention has a large number of active sites on the surface, has high catalytic activity, combines the synergistic effect of bimetal, and achieves the effects of high-efficiency broad-spectrum antibacterial performance and promoting wound healing. The compound has oxidase and catalase activities, and pharmaceutically acceptable salts and solvates thereof have good antibacterial performance and wound healing promoting effects on preparing antibacterial agents.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other related drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is an X-ray diffraction spectrum of the nano-enzyme provided by the invention;
FIG. 2 shows ZnO-Co provided by the invention 3 Schematic diagram of bacteriostasis performance of O nano enzyme;
FIG. 3 is a graph showing the results of treatment of wounds of mice in the control group, group 1 to group 3, provided by the present invention;
FIG. 4 is a flowchart of a preparation method of a nano-enzyme according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of a nanoenzyme provided by the invention.
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention.
The specific conditions were not specified in the examples, and the examples were conducted under the conventional conditions or the conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention. In addition, the meaning of "and/or" as it appears throughout includes three parallel schemes, for example "A and/or B", including the A scheme, or the B scheme, or the scheme where A and B are satisfied simultaneously. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be regarded as not exist and not within the protection scope of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Reactive Oxygen Species (ROS) are oxygen-containing highly reactive chemicals having unpaired electrons including superoxide anions (O2) .- ) Radical of hydroxy radical . OH)、H 2 O 2 And the like, ROS can cause damage to microbial DNA, proteins, and other chemicals, ultimately leading to cell death. The nano-enzyme can regulate ROS level and is used for developing novel antibacterial medicines. MSN-AuNPs can generate excessive ROS, thereby generating powerful antibacterial properties. Has obvious inhibiting effect on proliferation of gram-positive staphylococcus aureus and gram-negative escherichia coli. The nanoenzyme can be used as mimic of peroxidase for inhibiting wound infection caused by bacteria and promoting wound healing. For example, both the magnetic nanoparticles and the graphene quantum dots have peroxide mimic enzyme activity and thus have excellent antibacterial properties in wound infection. At present, bacterial drug resistance has become a worldwide problem, and nano-enzyme can be developed into a novel antibacterial agent, so that a novel thought is provided for solving the problem. In view of this, the present invention provides a ZnO-Co 3 O 4 Heterostructured nano-enzymes.
Referring to fig. 5, the present invention provides a nano-enzyme, which has a cubic structure, wherein C, zn and Co are uniformly dispersed in the cubic structure. That is, C, zn and Co are uniformly distributed to constitute the nano-enzyme. Referring to parts a and B of fig. 5, a Scanning Electron Microscope (SEM) of the nanoenzyme is shown, and the nanoenzyme has a shape of independent cubes. A Transmission Electron Microscope (TEM) image of the nanoenzyme in the C part clearly shows ZnO (100), znO (101) and Co 3 O 4 (511) Is a lattice fringe of D is ZnO-Co 3 O 4 Element Mapping (Mapping) of (a), which demonstrates ZnO-Co 3 O 4 The elements O, zn and Co of the (B) are uniformly dispersed in the cube structure. In summary, successful production of nanoenzymes was demonstrated.
Furthermore, the invention also provides a preparation method of the nano-enzyme, which comprises the following steps:
s10, adding zinc salt and 2-methylimidazole into water, mixing and stirring uniformly, and then performing centrifugal treatment to obtain a precipitate;
in particular, the zinc salt is preferably Zn (NO 3 ) 2 ·6H 2 And O, the parameters of the centrifugal treatment are as follows: centrifuging for 10 min-15 min at the rotation speed of 9000 rpm-10000 rpm.
Further, before the step S10 and the step S20, the method further includes washing the precipitate with deionized water.
S20, adding cobalt salt into a methanol solution, performing ultrasonic treatment, then adding the precipitate, and stirring to obtain a first solution;
in particular, the cobalt salt is preferably Co (NO 3 ) 2 ·6H 2 O。
S30, adding 2-methylimidazole into the methanol solution, and performing ultrasonic treatment to obtain a second solution;
s40, adding the second solution into the first solution, standing, centrifuging, drying and calcining to obtain the heterostructure nano-enzyme.
Specifically, the drying and calcining comprise drying at 70-75 ℃ for 8-12 hours, and calcining at 400-410 ℃ for 2-3 hours at a heating rate of 2-3 ℃/min. And standing for 8-24 hours, centrifuging, and washing with methanol after centrifuging.
Furthermore, the invention also provides a bacteriostatic agent comprising nano-enzyme or pharmaceutically acceptable salt thereof. The nano-enzyme has high catalytic activity, combines the synergistic effect of bimetal, and achieves the effects of high-efficiency broad-spectrum antibacterial performance and promoting wound healing. The compound has oxidase and peroxidase activities, and pharmaceutically acceptable salts and solvates thereof have good antibacterial performance and wound healing promoting effects on preparing antibacterial agents.
Specifically, the antibacterial agent can be introduced into a body such as muscle, subcutaneous, intradermal, intravenous, mucosal tissue by injection, nasal drip, eye drip, permeation, absorption, physical or chemical mediation, or introduced into the body after being mixed or wrapped with other substances, and has good effects.
Specifically, the bacteriostatic agent further comprises a compound solvent of the nano-enzyme, and the concentration of the nano-enzyme in the bacteriostatic agent is 1 mg/mL-3 mg/mL. Wherein the complexing solvent is preferably hydrogen peroxide (H) 2 O 2 ）。
Furthermore, the invention also provides application of various nano enzymes or salts thereof in preparing a bacteriostatic agent, and the bacteriostatic agent is preferably the bacteriostatic agent. In particular to application of the nano-enzyme or pharmaceutically acceptable salt thereof in preparing medicines for inhibiting gram-positive bacteria and gram-negative bacteria.
The following technical solutions of the present invention will be described in further detail with reference to specific examples and drawings, and it should be understood that the following examples are only for explaining the present invention and are not intended to limit the present invention.
Example 1: application of verified nano-enzyme as bacteriostat
1. Preparation of nanoenzyme
(1) Zn (NO) 3 ) 2 ·6H 2 Adding O and 2-methylimidazole into water, mixing and stirring, centrifuging at 9000rpm for 10min to obtain precipitate, and washing the precipitate with deionized water;
(2) Co (NO) 3 ) 2 ·6H 2 Adding O into a methanol solution, performing ultrasonic treatment, adding the precipitate, and stirring to obtain a first solution;
(3) And adding the second solution into the first solution, standing for 24 hours, centrifuging, washing with methanol, drying at 70 ℃ for 12 hours, and calcining at 400 ℃ for 2 hours at a heating rate of 2 ℃/min to obtain the nano-enzyme.
2. Plate count.
Diluting activated Staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and Bacillus subtilis to 10 in serial ratio 6 CFU/mL. 2mg/mL ZnO-Co 3 O 4 Heterojunction nano-enzyme, 5mmol/L H 2 O 2 The mixture was incubated with the bacterial suspension at 37℃for 2 hours, and the mixture was spread on a solid medium uniformly with a spreader which had been burned with alcohol and cooled to a temperature of the culture medium, and incubated overnight in a constant temperature incubator at 37℃ (about 18-24 h). Colonies on the plates were counted.
3. Group culture
Control group: 100 [ mu ] L of culture medium and 100 [ mu ] L of bacteria liquid;
group 1:50 mu L culture medium+100 mu L bacterial liquid+50 mu L ZnO-Co 3 O 4 (2 mg/mL)；
Group 2:50 mu L culture medium +50 mu L H 2 O 2 (5 mmol/L) +100 [ mu ] L bacterial liquid;
group 3:50 mu LH 2 O 2 (5 mmol/L) +100 [ mu ] L bacterial liquid+50 [ mu ] L ZnO-Co 3 O 4 (2 mg/mL)。
4. Sterilization rate= (number of colonies of control group-number of colonies of experiment group)/number of colonies of control group 100%
5. Promote wound healing of mice.
6mm diameter wounds were created on the back skin of mice after 7 days of adaptive feeding and infected with staphylococcus aureus, with a concentration of nanoenzyme and H 2 O 2 Dosing, mice were monitored daily for body weight, spirit, food intake, observed for wound healing and photographed for recording.
The invention is further described below with reference to the accompanying drawings.
(1) Please refer to fig. 1, which shows ZnO-Co 3 O 4 X-ray diffraction spectrum (XRD) of (a), wherein diffraction peaks (100), (002), (101), (102), (110), (103), (112), (201), (202) are ascribed to ZnO, diffraction peaks (111), (400), (511), (440) are ascribed to Co 3 O 4 XRD spectra illustrate ZnO-Co 3 O 4 Has a good crystal structure.
(2) Referring to FIG. 2, the nano-enzyme bacteriostatic agent ZnO-Co is shown 3 O 4 Results of plate count method for four species of staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, bacillus subtilis. Wherein, the sterilization rates of group 2, group 3 and group 4 on gram-negative bacteria such as escherichia coli are 74.06 percent, 83.33 percent and 98.34 percent respectively, which shows that 50 mu L of ZnO-Co 3 O 4 (2 mg/mL) to 5mmol/L H 2 O 2 And 50 [ mu ] L, 5mmol/L H 2 O 2 Can kill a part of escherichia coli, and 50 mu L of nano-enzyme bacteriostat ZnO-Co 3 O 4 (2 mg/mL) and 50 [ mu ] L, 5mmol/L H 2 O 2 The escherichia coli can be almost completely killed. The same bactericidal effect is also reflected in staphylococcus aureus, pseudomonas aeruginosa and bacillus subtilis.
(3) Creating a wound model on the back of a mouse by taking staphylococcus aureus as an infectious strain, referring to fig. 3, a nano-enzyme bacteriostatic agent ZnO-Co 3 O 4 Therapeutic pictures to inhibit bacteria and promote wound healing. Wherein, compared with the control group, it is obvious that the physiological saline and H are used 2 O 2 The capability of promoting wound healing and repairing is not obvious when the wound is treated, but the wound is treated by the nano-enzyme bacteriostatic agent ZnO-Co 3 O 4 And H 2 O 2 After treatment, not only effectively inhibit staphylococcus aureus, but alsoAccelerating wound healing.
In summary, the invention provides a method for preparing the nano-enzyme bacteriostat of the heterojunction, which can efficiently catalyze H 2 O 2 The ROS are generated by decomposition. At low concentration of H 2 O 2 Good bacteriostasis against gram-negative and gram-positive bacteria is achieved in the presence. The nano enzyme bacteriostatic agent is a nano enzyme material formed by low-temperature molten salt method and high-temperature calcination of bimetallic oxide, and can catalyze H 2 O 2 The ROS with super-strong sterilization capability is decomposed, and the synergistic catalytic effect among the bimetallic oxides is combined, so that the catalytic effect of the nano-enzyme is greatly enhanced, and the aim of high-efficiency sterilization is fulfilled.
Example 2: preparation of nanoenzyme
(1) Zn (NO) 3 ) 2 ·6H 2 Adding O and 2-methylimidazole into water, mixing and stirring, centrifuging at 9000rpm for 15min to obtain precipitate, and washing the precipitate with deionized water;
(2) Co (NO) 3 ) 2 ·6H 2 Adding O into a methanol solution, performing ultrasonic treatment, adding the precipitate, and stirring to obtain a first solution;
(3) And adding the second solution into the first solution, standing for 12 hours, centrifuging, washing with methanol, drying at 75 ℃ for 8 hours, and calcining at 400 ℃ for 2 hours at a heating rate of 2 ℃/min to obtain the nano-enzyme.
Example 3: preparation of nanoenzyme
(1) Zn (NO) 3 ) 2 ·6H 2 Adding O and 2-methylimidazole into water, mixing and stirring, centrifuging at 10000rpm for 10min to obtain precipitate, and washing the precipitate with deionized water;
(2) Co (NO) 3 ) 2 ·6H 2 Adding O into a methanol solution, performing ultrasonic treatment, adding the precipitate, and stirring to obtain a first solution;
(3) And adding the second solution into the first solution, standing for 12 hours, centrifuging, washing with methanol, drying at 70 ℃ for 10 hours, and calcining at 410 ℃ for 2 hours at a heating rate of 3 ℃/min to obtain the nano-enzyme.
The foregoing is merely a preferred embodiment of the present invention and is not intended to limit the scope of the present invention, but various modifications and variations will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (6)
1. The preparation method of the nano enzyme is characterized by comprising the following steps:
adding zinc salt and 2-methylimidazole into water, mixing and stirring uniformly, and then carrying out centrifugal treatment to obtain a precipitate;
the parameters of the centrifugal treatment are as follows:
centrifuging for 10 min-15 min at the rotation speed of 9000 rpm-10000 rpm;
adding cobalt salt into methanol solution, performing ultrasonic treatment, adding the precipitate, and stirring to obtain a first solution;
adding 2-methylimidazole into a methanol solution, and performing ultrasonic treatment to obtain a second solution;
adding the second solution into the first solution, standing, centrifuging, drying and calcining to obtain nano enzyme;
the drying calcination includes:
drying at 70-75 ℃ for 8-12 h, and calcining at 400-410 ℃ for 2-3 h at a heating rate of 2-3 ℃/min.
2. The method for preparing nano-enzyme according to claim 1, wherein zinc salt and 2-methylimidazole are added into water, mixed and stirred uniformly, and then centrifuged, and the precipitate is washed with deionized water in the step of taking the precipitate.
3. The method for preparing nano-enzyme according to claim 1, wherein the second solution is added into the first solution, and the nano-enzyme is obtained by standing, centrifuging, drying and calcining, and the step of washing with methanol is performed after the standing, centrifuging.
4. The method for preparing nano-enzyme according to claim 1, wherein the second solution is added into the first solution, and the second solution is subjected to standing centrifugation, and the precipitate is dried and calcined, so that the nano-enzyme is obtained, wherein the standing time is 8-24 hours.
5. A bacteriostatic agent comprising a nanoenzyme produced by the method of producing a nanoenzyme according to any one of claims 1 to 4.
6. The bacteriostat of claim 5, further comprising a complexing solvent for the nanoenzyme, wherein the concentration of the nanoenzyme in the bacteriostat is 1mg/mL to 3mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211002839.XA CN115463151B (en) | 2022-08-18 | 2022-08-18 | Nano-enzyme, preparation method and application thereof, and bacteriostat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211002839.XA CN115463151B (en) | 2022-08-18 | 2022-08-18 | Nano-enzyme, preparation method and application thereof, and bacteriostat |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115463151A CN115463151A (en) | 2022-12-13 |
| CN115463151B true CN115463151B (en) | 2023-09-19 |
Family
ID=84368200
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211002839.XA Active CN115463151B (en) | 2022-08-18 | 2022-08-18 | Nano-enzyme, preparation method and application thereof, and bacteriostat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115463151B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109758474A (en) * | 2019-03-06 | 2019-05-17 | 大连民族大学 | A kind of Ag/ZIF-8 antibacterial agent and preparation method thereof |
| CN111217401A (en) * | 2020-01-14 | 2020-06-02 | 扬州大学 | Copper-cobalt-sulfur nano enzyme material, preparation method and antibacterial application thereof |
| CN113181358A (en) * | 2021-03-19 | 2021-07-30 | 中国科学技术大学附属第一医院 | Application of Fe-N-C monatomic nanoenzyme |
| CN113237935A (en) * | 2021-04-29 | 2021-08-10 | 南京工业大学 | Synthesis method of C-N @ GC nano material and detection application of C-N @ GC nano material in cysteine |
| CN113499474A (en) * | 2021-05-31 | 2021-10-15 | 浙江大学 | ZIF-67 modified hollow vanadium dioxide shell-core structure micro-nano composite and preparation method and application thereof |
| CN114306382A (en) * | 2022-03-11 | 2022-04-12 | 南京大学 | Copper-based nanoenzyme and preparation method and application thereof |
| CN114345349A (en) * | 2022-01-07 | 2022-04-15 | 南开大学 | Preparation method and application of bimetallic nanoenzyme |
| CN114367298A (en) * | 2021-12-23 | 2022-04-19 | 首都师范大学 | Double-enzyme active cobalt monoatomic nano enzyme and preparation method and application thereof |
| CN114377715A (en) * | 2022-01-12 | 2022-04-22 | 山西大学 | Cobalt-doped carbon dot nanoenzyme and preparation method and application thereof |
| CN114835913A (en) * | 2022-05-24 | 2022-08-02 | 扬州大学 | Colorimetric analysis method of copper-cobalt bimetallic organic framework nanoenzyme and organophosphorus colorimetric sensor thereof |
| CN114904535A (en) * | 2022-04-26 | 2022-08-16 | 天津师范大学 | Preparation method of nano enzyme with activity superior to natural laccase |
-
2022
- 2022-08-18 CN CN202211002839.XA patent/CN115463151B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109758474A (en) * | 2019-03-06 | 2019-05-17 | 大连民族大学 | A kind of Ag/ZIF-8 antibacterial agent and preparation method thereof |
| CN111217401A (en) * | 2020-01-14 | 2020-06-02 | 扬州大学 | Copper-cobalt-sulfur nano enzyme material, preparation method and antibacterial application thereof |
| CN113181358A (en) * | 2021-03-19 | 2021-07-30 | 中国科学技术大学附属第一医院 | Application of Fe-N-C monatomic nanoenzyme |
| CN113237935A (en) * | 2021-04-29 | 2021-08-10 | 南京工业大学 | Synthesis method of C-N @ GC nano material and detection application of C-N @ GC nano material in cysteine |
| CN113499474A (en) * | 2021-05-31 | 2021-10-15 | 浙江大学 | ZIF-67 modified hollow vanadium dioxide shell-core structure micro-nano composite and preparation method and application thereof |
| CN114367298A (en) * | 2021-12-23 | 2022-04-19 | 首都师范大学 | Double-enzyme active cobalt monoatomic nano enzyme and preparation method and application thereof |
| CN114345349A (en) * | 2022-01-07 | 2022-04-15 | 南开大学 | Preparation method and application of bimetallic nanoenzyme |
| CN114377715A (en) * | 2022-01-12 | 2022-04-22 | 山西大学 | Cobalt-doped carbon dot nanoenzyme and preparation method and application thereof |
| CN114306382A (en) * | 2022-03-11 | 2022-04-12 | 南京大学 | Copper-based nanoenzyme and preparation method and application thereof |
| CN114904535A (en) * | 2022-04-26 | 2022-08-16 | 天津师范大学 | Preparation method of nano enzyme with activity superior to natural laccase |
| CN114835913A (en) * | 2022-05-24 | 2022-08-02 | 扬州大学 | Colorimetric analysis method of copper-cobalt bimetallic organic framework nanoenzyme and organophosphorus colorimetric sensor thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115463151A (en) | 2022-12-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101731272B (en) | Method for preparing antibacterial nanometer silver colloid | |
| CN110051837B (en) | CuO/ZnO/Au nano-particles and preparation method and application thereof | |
| CN109364100A (en) | A kind of nanometer vulcanization iron mixture of high-efficiency antimicrobial and its preparation method and application | |
| CN112795202B (en) | MOF composite material with antibacterial function and preparation method and application thereof | |
| CN112850686B (en) | Fenton-like copper monoatom/aza-carbon nanomaterial and preparation method and application thereof | |
| CN111001002A (en) | Polydopamine-coated Prussian blue silver-loaded nano composite material and preparation method and application thereof | |
| CN109260222A (en) | Cysteine base antimicrobial nano vulcanizes iron mixture and its preparation method and application | |
| CN110496219A (en) | A kind of synthetic method of novel ferrihydrite nanometric photosensitizer and its application in anticancer antibiotic | |
| CN108042809A (en) | A kind of preparation method of bacteria resistance function graphene oxide | |
| CN114191562B (en) | Preparation method and application of double-enzyme-activity antibacterial material | |
| CN1568704A (en) | Composite nanometer antibiotic material, preparation method and products thereof | |
| CN113559262B (en) | Photodynamic nano antibacterial material and preparation method and application thereof | |
| CN107549476A (en) | A kind of high antimicrobial nano feed addictive and preparation method thereof | |
| Xie et al. | Ti-MOF-based biosafety materials for efficient and long-life disinfection via synergistic photodynamic and photothermal effects | |
| CN112209445B (en) | Preparation method and application of molybdenum trioxide nanodot antibacterial material | |
| CN110527756A (en) | A kind of environment-friendly highly efficient leather nano antibacterial agent and its preparation method and application | |
| CN115463151B (en) | Nano-enzyme, preparation method and application thereof, and bacteriostat | |
| CN111803695B (en) | Preparation method of NO release type wound dressing based on silver-doped carbon dots, product and application thereof | |
| CN116851741A (en) | Hollow mesoporous single-atom molybdenum nano-enzyme, preparation method thereof, preparation method of nano-enzyme reactor based on hollow mesoporous single-atom molybdenum nano-enzyme and application of nano-enzyme reactor | |
| Agnes et al. | Preparation and characterization studies of chitosan encapsulated ZnO nanoparticles modified with folic acid and their antibacterial activity against selected bacterial species | |
| CN114534758B (en) | Bismuth ferrite/graphite phase carbon nitride composite material and preparation method and application thereof | |
| CN114732906A (en) | Barium titanate-graphene composite material and preparation method and application thereof | |
| CN112168809A (en) | Novel nano-silver antibacterial material and preparation method and application thereof | |
| CN115475244B (en) | Metal organic framework nano-composite and preparation method and application thereof | |
| CN114711251B (en) | Titanium carbide-manganese sulfide composite antibacterial material, preparation method thereof and antibacterial method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |