CN113237935A - Synthesis method of C-N @ GC nano material and detection application of C-N @ GC nano material in cysteine - Google Patents

Synthesis method of C-N @ GC nano material and detection application of C-N @ GC nano material in cysteine Download PDF

Info

Publication number
CN113237935A
CN113237935A CN202110472486.9A CN202110472486A CN113237935A CN 113237935 A CN113237935 A CN 113237935A CN 202110472486 A CN202110472486 A CN 202110472486A CN 113237935 A CN113237935 A CN 113237935A
Authority
CN
China
Prior art keywords
zif
solution
cysteine
methanol
room temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202110472486.9A
Other languages
Chinese (zh)
Other versions
CN113237935B (en
Inventor
王志娟
蔡永亮
陈剑
刘霞
胡松
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Tech University
Original Assignee
Nanjing Tech University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Tech University filed Critical Nanjing Tech University
Priority to CN202110472486.9A priority Critical patent/CN113237935B/en
Publication of CN113237935A publication Critical patent/CN113237935A/en
Application granted granted Critical
Publication of CN113237935B publication Critical patent/CN113237935B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Carbon And Carbon Compounds (AREA)

Abstract

The invention discloses a synthesis method of a C-N @ GC nano material and a detection application of the C-N @ GC nano material in cysteine. In the present invention, a methanol solution of 2-methylimidazole and Zn (NO)2·6H2Stirring and mixing the O methanol solution at room temperature to obtain a precipitate ZIF-8, and centrifuging, washing and drying the precipitate to obtain a white powder product; dispersing synthesized ZIF-8 in methanol solution with ultrasonic wave, stirring for 10min, mixing methanol solution of 2-methylimidazole with Co (NO)2·6H2Pouring a methanol solution of O into the solution; stirring the mixed solution at room temperature to obtain a precipitate ZIF-8@ ZIF-67, and centrifuging, washing and drying the precipitate to obtain a purple powder product; taking a certain amount of ZIF-8@ ZIF-67, placing in a muffle furnace, heating at a heating rate of 2 ℃ per minute in Ar atmosphere, calcining at a constant temperature of 920 ℃ for 2h, and cooling to room temperature to obtain a black solidThe body C-N @ GC; and modifying the surface of a glassy carbon electrode by using C-N @ GC for cysteine detection. The invention promotes the application of the nano modified electrode in cysteine detection.

Description

Synthesis method of C-N @ GC nano material and detection application of C-N @ GC nano material in cysteine
Technical Field
The invention relates to the technical field of electroanalysis, in particular to a synthesis method of a C-N @ GC nano material and a detection application of the C-N @ GC nano material in cysteine.
Background
Cysteine (Cys) is a small thiol-containing molecule that, because of its chemical instability, plays a variety of roles in physiological and biological processes. In biological processes, cysteine is a precursor for the synthesis of glutathione, acetyl-coa, cystine, and the like. It can undergo reversible redox reactions under physiological conditions, which is essential for maintaining the tertiary and quaternary structure of proteins. In addition, cysteine can be catalytically metabolized as a substrate into bioactive molecules such as pyruvic acid, sulfenic acid, hydrogen sulfide, and the like, and further, can exert various functions in vivo. Cysteine deficiency in humans can lead to hair loss, slow growth, liver damage, edema, weakness, and skin damage. Elevated cysteine levels are also directly associated with several diseases, including L-cystinuria, aids and cancer. Therefore, it is essential to maintain normal levels of cysteine in humans, and plays a crucial role in maintaining human health.
At present, there are many methods for detecting cysteine, mainly including: fluorescence spectroscopy, colorimetry, chromatography, capillary electrophoresis, chemiluminescence, and the like. Although these methods achieve detection of cysteine, they also have problems such as long detection time, expensive equipment and equipment. The electrochemical sensor is the preferred method for detecting cysteine due to the advantages of simplicity, high sensitivity, easy operation, miniaturization and the like.
Disclosure of Invention
1. Technical problem to be solved
The invention aims to provide a synthesis method of a C-N @ GC nano material and detection application of the C-N @ GC nano material in cysteine, so as to solve the problems of long detection time, expensive required instruments and equipment and the like in the background technology.
2. Technical scheme
In order to solve the problems, the invention adopts the following technical scheme:
a method for synthesizing a C-N @ GC nano material comprises the following steps:
(1) synthesis of ZIF-8: the methanol solution of 2-methylimidazole is poured into Zn (NO)2·6H2In methanol solution of O; secondly, stirring the mixed solution at room temperature for 24 hours to obtain milky white liquid; then washing the milky white liquid with methanol for 3 times, and centrifugally collecting the milky white liquid by a centrifugal machine; finally, vacuum drying at 60 ℃ overnight to obtain ZIF-8;
(2) synthesis of ZIF-8@ ZIF-67: taking ZIF-8 and dispersing in methanol solution with ultrasonic wave, stirring for 10min to obtain ZIF-8 solution, mixing methanol solution of 2-methylimidazole with Co (NO)2·6H2Pouring the methanol solution of O into the ZIF-8 solution to obtain a mixed solution; secondly, stirring the mixed solution at room temperature for 24 hours to obtain a purple solution; then washing the purple solution with methanol for 3 times, and centrifuging and collecting the solution by a centrifuge; finally, vacuum drying at 60 ℃ overnight to obtain ZIF-8@ ZIF-67;
(3) synthesis of C-N @ GC: and (3) putting the ZIF-8@ ZIF-67 synthesized in the step (2) into a muffle furnace, heating at a heating rate of 2 ℃ per minute in an Ar atmosphere, calcining at a constant temperature of 920 ℃ for 2 hours, and cooling the finished product to room temperature to obtain a black solid C-N @ GC nano material.
Further, in the step (1) of synthesizing ZIF-8, 2-methylimidazole reacts with Zn (NO)2·6H2The mass ratio of O is 6.16: 5.95.
further, in the process of synthesizing ZIF-8@ ZIF-67 in the step (2), 2-methylimidazole, Co (NO)2·6H2The mass ratio of O to ZIF-8 is 6.16: 5.82: 0.5.
the application of the C-N @ GC nano material in cysteine detection comprises the following steps:
s1, preparation of modifier solution: dispersing C-N @ GC in a water-ethanol solution, dropwise adding a naphthol solution with the weight percentage of 5 wt% into the solution, and performing ultrasonic treatment for 2 hours by using an ultrasonic degradation method to obtain a uniform modifier solution;
s2, modification of the surface of the glassy carbon electrode: uniformly dripping the modifier solution in the S1 on the surface of the polished glassy carbon electrode, and drying at room temperature to finally obtain the glassy carbon electrode modified by C-N @ GC;
s3, the glassy carbon electrode modified by the C-N @ GC obtained in the S2 is used for electrochemical detection of cysteine.
Further, before the modifying agent solution is prepared in S1, the method further comprises the steps of: the C-N @ GC was transferred to a mortar, ground well to a powder, and the ground C-N @ GC was put into a water-ethanol solution.
3. Advantageous effects
Compared with the defects and shortcomings of the prior art, the invention has the following beneficial effects: the invention aims to develop a method for electrochemically detecting cysteine, which has the advantages of simple synthesis, high sensitivity and quick detection. According to the invention, ZIF-8@ ZIF-67 is taken as a precursor, and the product C-N @ GC can be obtained through carbonization, so that the product is stable, the preparation method is simple and convenient, the cost is lower, and the industrial production is easy to realize. The C-N @ GC prepared by the invention contains metal nano particles, so that the conductivity of the material is increased, and the detection capability of the C-N @ GC is improved due to the synergistic effect between C-N and GC in the C-N @ GC. The invention can realize qualitative and quantitative detection of cysteine, provides a new idea and a new method for discovering and constructing a novel electrochemical detection probe, and promotes the development and application of an electrochemical method in cysteine detection.
Drawings
FIG. 1 shows the detection result of cysteine by a glassy carbon electrode modified by C-N @ GC;
FIG. 2 shows the detection result of a glassy carbon electrode modified by C-N @ GC and containing no cysteine solution.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Example 1
A method for synthesizing a C-N @ GC nano material comprises the following steps:
(1) synthesis of ZIF-8: a methanol solution of 2-methylimidazole (6.16g,150mL) was poured into Zn (NO)2·6H2Of OMethanol solution (5.95g, 150 mL); secondly, stirring the mixed solution at room temperature for 24 hours to obtain milky white liquid; then washing the milky white liquid with methanol for 3 times, and centrifugally collecting the milky white liquid by a centrifugal machine; finally, vacuum drying at 60 ℃ overnight to obtain ZIF-8; and 2-methylimidazole with Zn (NO)2·6H2The mass ratio of O is 6.16: 5.95;
(2) synthesis of ZIF-8@ ZIF-67: the ZIF-8(0.5g) synthesized in step (1) was dispersed in a methanol solution (100mL) by ultrasonic wave, and after stirring for 10min, a methanol solution of 2-methylimidazole (6.16g,100mL) and Co (NO)2·6H2A methanol solution of O (5.82g,100mL) was poured into the above solution; secondly, stirring the mixed solution at room temperature for 24 hours to obtain a purple solution; then washing the purple solution with methanol for 3 times, and centrifuging and collecting the solution by a centrifuge; finally, vacuum drying at 60 ℃ overnight to obtain ZIF-8@ ZIF-67; and 2-methylimidazole, Co (NO)2·6H2The mass ratio of O to ZIF-8 is 6.16: 5.82: 0.5;
(3) synthesis of C-N @ GC: and (3) putting a certain amount of ZIF-8@ ZIF-67 synthesized in the step (2) into a muffle furnace, heating at a heating rate of 2 ℃ per minute in Ar atmosphere, calcining at a constant temperature of 920 ℃ for 2 hours, and cooling the finished product to room temperature to obtain a black solid C-N @ GC nano material.
Example 2
The application of the C-N @ GC nano material in cysteine detection comprises the following steps:
s1, preparation of modifier solution: taking 5 mg of the C-N @ GC synthesized in the example 1, transferring the C-N @ GC into a mortar, fully grinding the C-N @ GC into powder, putting the ground C-N @ GC into 1 ml of water-ethanol solution (volume ratio is 3:1), uniformly dispersing the ground C-N @ GC into 1 ml of water-ethanol solution (volume ratio is 3:1), dropwise adding 20 mu L of naphthol solution (5 wt%), and performing ultrasonic degradation for 2 hours by using an ultrasonic degradation method to obtain a uniform modifier solution;
s2, modification of the surface of the glassy carbon electrode: uniformly dripping 3 mu L of modifier solution in S1 on the surface of the polished glassy carbon electrode, and drying at room temperature to finally obtain the glassy carbon electrode modified by C-N @ GC;
s3, the glassy carbon electrode modified by the C-N @ GC obtained in the S2 is used for electrochemical detection of cysteine.
The C-N @ GC modified glassy carbon electrode has the specific application process in the electrochemical detection of cysteine as follows: CV tests (scan rate 50 mVs) were performed with a C-N @ GC modified glassy carbon electrode (GCE, 3 mM diameter), Pt sheet and Ag/AgCl electrode (saturated KCl) as the Working Electrode (WE), counter electrode (RE) and reference electrode (CE) for electrochemical detection, respectively, in a solution containing cysteine (5 mM PBS (pH 7.3))-1) (ii) a As shown in FIG. 1, a distinct oxidation peak can be observed on the CV curve in the presence of cysteine, indicating that the C-N @ GC modified glassy carbon electrode can detect cysteine;
comparative example
The glassy carbon electrode modified by C-N @ GC is applied to the electrochemical detection of other amino acids, and the specific application process is as follows: CV tests (scan rate of 50 mVs) were performed with a glassy carbon electrode (GCE, 3 mM diameter), Pt sheet and Ag/AgCl electrode (saturated KCl) modified with C-N @ GC as a Working Electrode (WE), counter electrode (RE) and reference electrode (CE) for electrochemical detection, respectively, in a (2mM PBS (pH 7.3)) solution containing glycine, alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, tyrosine, aspartic acid, histidine, asparagine, glutamic acid, lysine, glutamine, arginine, serine, threonine, proline and methionine-1) (ii) a As shown in fig. 2, no oxidation absorption peak was observed in these CV curves, indicating that the 19 natural amino acids described above have no electrochemical response on the C-N @ GC modified glassy carbon electrode;
the invention can realize qualitative and quantitative detection of cysteine, provides a new idea and a new method for discovering and constructing a novel electrochemical detection probe, and promotes the development and application of an electrochemical method in cysteine detection.
It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that changes and modifications to the above embodiments are within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.

Claims (5)

1. A method for synthesizing a C-N @ GC nano material is characterized by comprising the following steps:
(1) synthesis of ZIF-8: the methanol solution of 2-methylimidazole is poured into Zn (NO)2·6H2In methanol solution of O; secondly, stirring the mixed solution at room temperature for 24 hours to obtain milky white liquid; then washing the milky white liquid with methanol for 3 times, and centrifugally collecting the milky white liquid by a centrifugal machine; finally, vacuum drying at 60 ℃ overnight to obtain ZIF-8;
(2) synthesis of ZIF-8@ ZIF-67: taking ZIF-8 and dispersing in methanol solution with ultrasonic wave, stirring for 10min to obtain ZIF-8 solution, mixing methanol solution of 2-methylimidazole with Co (NO)2·6H2Pouring the methanol solution of O into the ZIF-8 solution to obtain a mixed solution; secondly, stirring the mixed solution at room temperature for 24 hours to obtain a purple solution; then washing the purple solution with methanol for 3 times, and centrifuging and collecting the solution by a centrifuge; finally, vacuum drying at 60 ℃ overnight to obtain ZIF-8@ ZIF-67;
(3) synthesis of C-N @ GC: and (3) putting the ZIF-8@ ZIF-67 synthesized in the step (2) into a muffle furnace, heating at a heating rate of 2 ℃ per minute in an Ar atmosphere, calcining at a constant temperature of 920 ℃ for 2 hours, and cooling the finished product to room temperature to obtain a black solid C-N @ GC nano material.
2. The method for synthesizing C-N @ GC nanomaterial according to claim 1, wherein 2-methylimidazole reacts with Zn (NO) during the synthesis of ZIF-8 in step (1)2·6H2The mass ratio of O is 6.16: 5.95.
3. the method for synthesizing C-N @ GC nanomaterial according to claim 1, wherein in the step (2) of synthesizing ZIF-8@ ZIF-67, 2-methylimidazole, Co (NO)2·6H2The mass ratio of O to ZIF-8 is 6.16: 5.82: 0.5.
the application of the C-N @ GC nanomaterial in cysteine detection is characterized by comprising the following steps of:
s1, preparation of modifier solution: dispersing C-N @ GC in a water-ethanol solution, dropwise adding a naphthol solution with the weight percentage of 5 wt% into the solution, and performing ultrasonic treatment for 2 hours by using an ultrasonic degradation method to obtain a uniform modifier solution;
s2, modification of the surface of the glassy carbon electrode: uniformly dripping the modifier solution in the S1 on the surface of the polished glassy carbon electrode, and drying at room temperature to finally obtain the glassy carbon electrode modified by C-N @ GC;
s3, the glassy carbon electrode modified by the C-N @ GC obtained in the S2 is used for electrochemical detection of cysteine.
5. The use of the C-N @ GC nanomaterial of claim 4, further comprising, prior to the formulation of the modifier solution in S1, the steps of: the C-N @ GC was transferred to a mortar, ground well to a powder, and the ground C-N @ GC was put into a water-ethanol solution.
CN202110472486.9A 2021-04-29 2021-04-29 Cysteine detection method Active CN113237935B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110472486.9A CN113237935B (en) 2021-04-29 2021-04-29 Cysteine detection method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110472486.9A CN113237935B (en) 2021-04-29 2021-04-29 Cysteine detection method

Publications (2)

Publication Number Publication Date
CN113237935A true CN113237935A (en) 2021-08-10
CN113237935B CN113237935B (en) 2022-06-28

Family

ID=77131463

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110472486.9A Active CN113237935B (en) 2021-04-29 2021-04-29 Cysteine detection method

Country Status (1)

Country Link
CN (1) CN113237935B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114479104A (en) * 2022-01-24 2022-05-13 辽宁大学 Reagent for detecting Pi and o-phenylenediamine based on visual series connection and detection method
CN115463151A (en) * 2022-08-18 2022-12-13 湖北文理学院 Nano enzyme, preparation method and application thereof, and bacteriostatic agent

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109244427A (en) * 2018-10-31 2019-01-18 北京科技大学 Carbon coating zinc sulphide loads preparation method of the graphene as kalium ion battery cathode
CN111440286A (en) * 2020-03-12 2020-07-24 济南大学 Preparation method and application of chiral Co-MOF/CoSR/PU core-shell composite material
CN112345607A (en) * 2020-10-12 2021-02-09 苏州健雄职业技术学院 Triazophos biosensor based on two-dimensional nano material

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109244427A (en) * 2018-10-31 2019-01-18 北京科技大学 Carbon coating zinc sulphide loads preparation method of the graphene as kalium ion battery cathode
CN111440286A (en) * 2020-03-12 2020-07-24 济南大学 Preparation method and application of chiral Co-MOF/CoSR/PU core-shell composite material
CN112345607A (en) * 2020-10-12 2021-02-09 苏州健雄职业技术学院 Triazophos biosensor based on two-dimensional nano material

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
QIANWU ET AL: "Turn Off-On Electrochemiluminescence Sensor Based on MnO2/Carboxylated Graphitic Carbon Nitride Nanocomposite for Ultrasensitive L-Cysteine Detection", 《JOURNAL OF THE ELECTROCHEMICAL SOCIETY》 *
XIA LIU ET AL: "Zeolitic imidazole framework derived N-doped porous carbon/metal cobalt nanoparticles hybrid for oxygen electrocatalysis and rechargeable Zn–air batteries", 《RSC ADV》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114479104A (en) * 2022-01-24 2022-05-13 辽宁大学 Reagent for detecting Pi and o-phenylenediamine based on visual series connection and detection method
CN115463151A (en) * 2022-08-18 2022-12-13 湖北文理学院 Nano enzyme, preparation method and application thereof, and bacteriostatic agent
CN115463151B (en) * 2022-08-18 2023-09-19 湖北文理学院 Nano-enzyme, preparation method and application thereof, and bacteriostat

Also Published As

Publication number Publication date
CN113237935B (en) 2022-06-28

Similar Documents

Publication Publication Date Title
CN113237935B (en) Cysteine detection method
US12130253B2 (en) Method for selective detection of L-tryptophan using formaldehyde as medium
CN106442686B (en) A kind of chiral metal organic framework polymer sensor and its preparation method and application
Bai et al. Voltammetric detection of riboflavin based on ordered mesoporous carbon modified electrode
CN108872344A (en) A kind of oxygen-enriched nano biologic enzyme electrode, sensor device and its preparation method and application
Kang et al. Hemin-doped metal–organic frameworks based nanozyme electrochemical sensor with high stability and sensitivity for dopamine detection
CN103808779A (en) Method for detecting p-nitrophenol by using PDDA (Poly Diallyldimethylammonium) functionalized graphene modified glassy carbon electrode
Babaei et al. A new sensor for simultaneous determination of tyrosine and dopamine using iron (III) doped zeolite modified carbon paste electrode
CN111351828B (en) ZnFe 2 O 4 Preparation method and application of modified electrode material
CN108872343A (en) A kind of Dopamine Sensor and its preparation and application based on nitrogen-doped graphene
CN110441294B (en) Co-coated based on ferritin3O4Preparation method of biosensor with core-shell structure
CN111103340A (en) Preparation method and application of glassy carbon electrode modified by nano particles
Cui et al. Cu–MOF/hemin: a bionic enzyme with excellent dispersity for the determination of hydrogen peroxide released from living cells
Ling et al. A metal–organic framework with multienzyme activity as a biosensing platform for real-time electrochemical detection of nitric oxide and hydrogen peroxide
Shi et al. Colorimetric determination of biothiols with AuNPs@ MoS 2 NSs as a peroxidase mimetic enzyme
Zhu et al. Differential pulse stripping voltammetric determination of metronidazole with graphene-sodium dodecyl sulfate modified carbon paste electrode
Salimi et al. Electrocatalytic oxidation of sulfur containing amino acids at renewable Ni‐powder doped carbon ceramic electrode: application to amperometric detection L‐cystine, L‐cysteine and L‐methionine
CN109187687B (en) Preparation of conjugated organic microporous material modified electrode and application of modified electrode as peroxynitroso anion electrochemical sensor
Wang et al. Fabrication of nitrogen-enriched carbon dots with green fluorescence for enzyme-free detection of uric acid
Zhang et al. Determination of glutathione based on NiPd nanoparticles mediated with acetaminophen
CN113092553A (en) Novel method for detecting glucose by enzyme-free glucose sensor
Lin et al. An ultrasensitive electrochemiluminescent d-alanine biosensor based on the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires
Beigi et al. Use of an electrogenerated chemiluminescence sensor modified with Sm2O3 nanoparticles/chitosan for the analysis of phenylalanine
Xiong et al. Supercritical fluid-assisted fabrication of CuxOSAS-based electrochemical sensor for cysteine detection with enhanced sensitivity and reduced oxidation potential
CN114878648B (en) Cysteine electrochemical sensor and preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant