CN115452743A - Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination - Google Patents

Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination Download PDF

Info

Publication number
CN115452743A
CN115452743A CN202211110573.0A CN202211110573A CN115452743A CN 115452743 A CN115452743 A CN 115452743A CN 202211110573 A CN202211110573 A CN 202211110573A CN 115452743 A CN115452743 A CN 115452743A
Authority
CN
China
Prior art keywords
phase
complex amplitude
wavelength
plane
sub
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211110573.0A
Other languages
Chinese (zh)
Inventor
左超
陈样
陈钱
吴雪娟
孙佳嵩
卢林芃
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing University of Science and Technology
Original Assignee
Nanjing University of Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing University of Science and Technology filed Critical Nanjing University of Science and Technology
Priority to CN202211110573.0A priority Critical patent/CN115452743A/en
Publication of CN115452743A publication Critical patent/CN115452743A/en
Priority to PCT/CN2023/092135 priority patent/WO2024055602A1/en
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts
    • G01N2021/0112—Apparatus in one mechanical, optical or electronic block

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microscoopes, Condenser (AREA)
  • Holo Graphy (AREA)

Abstract

The invention discloses a lensless single-frame phase recovery method based on partially coherent light emitting diode illumination. And then, directly recovering the high-resolution high signal-to-noise ratio phase information of the object by combining a multi-wavelength iterative phase recovery method with dynamic phase support constraint. The invention does not need to carry out complicated modification on the traditional lens-free on-chip microscope, and can endow the lens-free on-chip with the capability of fast and long-time single-frame phase recovery.

Description

Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination
Technical Field
The invention belongs to optical microscopic measurement and unmarked phase recovery technology, and particularly relates to a lensless single-frame phase recovery method based on partially coherent light emitting diode illumination.
Background
In the fields of cell biology, digital pathology, high-throughput drug screening and the like, the demand for observing and recording subcellular information of high-throughput biological samples is increasing day by day. However, the conventional microscope has difficulty in balancing the magnification of the objective lens with the imaging field of view (FOV), and cannot simultaneously achieve high resolution and large field of view imaging. Although the limitation of the scanning displacement platform and the splicing algorithm (Brown M, lowe D G. Automatic parallel image using in a variable features [ J ]. International journal of computer vision,2007,74 (1): 59-73.) can be broken through, the complexity of the system is greatly increased at the same time, and the system is difficult to be widely applied in biology and medical science.
In recent years, the advent and development of lensless on-chip holographic microscopes (LFOCM) have broken the limitations of field of view size (FOV) and resolution in conventional microscopes, with the unique advantage of being able to image on the original field of view of the imaging sensor without any imaging lenses and other intermediate optical elements. The resolution of a conventional lensless imaging system is limited by the sampling frequency and diffraction limit of the pixel, and the sensor pixel size is a major factor affecting the imaging resolution due to its effective Numerical Aperture (NA) close to 1. Therefore, in most lensless imaging systems, a high coherence laser source and a small pixel sensor have been used to obtain high resolution results. Meanwhile, in the field of pixel super-resolution, a plurality of methods such as active parallel plate scanning, axial scanning of a sample-to-sensor distance or multi-wavelength scanning using an ultra-wide laser are invented, and the limitation of the sampling frequency of a sensor pixel is broken through to obtain higher resolution.
However, in the field of optical phase microscopy, the adoption of partially coherent illumination has great significance for improving imaging quality and resolution and suppressing coherent noise. Thus, there have been many related works both in recent years and abroad, ozcan et al developed a miniaturized lens-free system using LED illumination (Pushkarsky, I., liu, Y., weaver, W.et al. Automated single-cell mobility analysis on a chip using lens from micro Optics. Sci. Rep4,4717 (2014). Https:// doi.org/10.1038/srep 04717), and Zuo et al realized experimental system using LED array illumination with no mechanical displacement (Zuo C, sun J, zhang J, et al. Light phase Optics and diffusion with multiple-angle and multiple-imaging Optics with simplified laser imaging 3763, 14311: simplified imaging of the entire LED from the LED array illumination: simplified imaging system [ 14363, 14363 ] and reduced noise. However, according to the theoretical analysis before Zuo et al (Zhang J, sun J, chen Q, et al. Resolution analysis in a lens-free on-chip digital pharmacological Microscope [ J ]. IEEE Transactions on Computational Imaging,2020, 6-697-710.. Insufficient space-time coherence of the LED light source causes diffraction high-frequency information to be mixed into a low-frequency area, so that extra resolution loss is caused, and even the sampling frequency of a replacement pixel becomes a main factor for limiting the imaging resolution.
In view of the above problems, in order to achieve higher resolution in a system of partially coherent illumination, until now, only a few efforts have compensated for the loss of resolution due to insufficient spatio-temporal coherence of the partially coherent light source, ozcan et al have compensated for the effect of temporal coherence on the imaging resolution using coherent modal decomposition (Sencan I, coskid a F, sikora U, et al, spectral subtraction in holovision and fluorescence on-chip microscopicity [ J ]. Scientific reports,2014,4 (1): 1-9.), and Feng et al have compensated to some extent the loss of resolution due to insufficient spatial coherence for a lens in-linear imaging with the resolution of the sample 3262, but they have performed simply on the sample 3262 using deconvolution methods (wing S, wu J. Resolution enhancement methods). Therefore, a single-frame phase recovery technique based on a lensless microscope on a chip illuminated by an LED and a long-term dynamic quantitative phase imaging observation of a biological sample have not been reported so far.
Disclosure of Invention
The invention aims to provide a lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination.
The technical scheme for realizing the purpose of the invention is as follows: a lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination comprises the following steps:
step 1, collecting a holographic image of a sample;
step 2, splitting the LED wide-spectrum wavelength according to spectrum prior, and initializing phase constraint information;
and 3, updating the complex amplitude of the iterative object by using the sub-wavelength of the digital segmentation and the shot hologram to obtain the object phase, wherein the method specifically comprises the following steps:
transmitting an original intensity image to a focus plane by using the sub-wavelength of a wide-spectrum LED to obtain object plane complex amplitude, and respectively implementing phase support constraint and uniform light intensity constraint on the phase and the amplitude of the complex amplitude;
and transmitting the updated complex amplitude back to the surface of the camera by utilizing an angular spectrum theory to obtain phase information of the current segmentation sub-wavelength transmitted back to the plane of the camera, updating the amplitude information of the surface of the camera according to the shot hologram and the spectrum prior weight coefficient, traversing all the sub-wavelengths and recovering the phase information.
Preferably, the holographic image is acquired by using a lensless on-chip microscope system, wherein the lensless on-chip microscope system comprises a single light-emitting diode illumination light source and a sensor, and the LED light source is a wide-spectrum patch LED illumination light source which is not completely monochromatic.
Preferably, step 2 is specifically:
the illumination wavelength λ is divided into N equally spaced quasi-monochromatic subwavelengths { λ ] according to the spectrum of the prior illuminating LED i ,i＝1,2,...,N}；
Using central wavelength lambda c A light intensity chart I to be shot ini Combining the initial zero phase psi 0 =0 generating camera plane complex amplitude
Figure BDA0003843885530000031
Propagating the complex amplitude back to the object plane and then using the complex amplitude of the object plane
Figure BDA0003843885530000032
The phase part of (1) obtains an initial phase constraint Mask 0 。
Preferably, the step of updating the complex amplitude of the iterative object by using the digitally divided sub-wavelengths and the photographed hologram to obtain the object phase comprises:
step 3.1, apply hologram I ini Combined with last wavelength of the splitter i-1 Phase information propagated back to the camera plane generates a camera plane complex amplitude;
step 3.2, transmitting the complex amplitude to the surface of the object by corresponding wavelength to obtain object surface complex amplitude;
step 3.3, applying phase support constraint to phase part of object plane complex amplitude
Figure BDA0003843885530000033
And updating the phase according to the wavelength
Figure BDA0003843885530000034
While applying uniform light intensity constraints to the amplitude portion
Figure BDA0003843885530000035
Wherein ave (-) is a mean calculation operator to obtain an updated complex amplitude
Figure BDA0003843885530000036
Step 3.4, the updated object plane complex amplitude
Figure BDA0003843885530000037
Propagating back to the camera plane to obtain a splitted sub-wavelength λ i The phase information propagated back to the camera plane returns to step 3.1, incorporating the next sub-wavelength λ i+1 The weights update the camera face complex amplitude until all the split sub-wavelengths are traversed, thereby obtaining the high resolution and high signal-to-noise ratio phase ψ of the sample.
Preferably, the camera face complex amplitude generated in step 3.1 is specifically:
Figure BDA0003843885530000038
in the formula (I), the compound is shown in the specification,
Figure BDA0003843885530000041
is the complex amplitude of the camera plane, omega i Is the weight of the ith sub-wavelength, j is the imaginary unit, ψ i For the last division sub-wavelength lambda i-1 Phase information is propagated back to the camera plane.
Preferably, the object plane complex amplitude obtained in step 3.2 is specifically:
Figure BDA0003843885530000042
wherein H (-d) is a transfer function of the propagation distance-d,
Figure BDA0003843885530000043
is the complex amplitude of the object plane,
Figure BDA0003843885530000044
for more than one sub-wavelength λ of division i-1 Propagating back to the mode of the complex amplitude behind the object plane,
Figure BDA0003843885530000045
is the object plane phase.
Compared with the prior art, the invention has the following remarkable advantages: (1) On the premise of using the patch LED to illuminate the simplified imaging system, the corresponding resolution result under the condition of using laser illumination can be obtained. (2) Only a single hologram needs to be shot, and no displacement platform or splicing algorithm is needed, so that the system is more compact, economical and efficient, and real-time long-time imaging under an ultra-large view field can be performed on living cells in an incubator.
The present invention is described in further detail below with reference to the attached drawing figures.
Drawings
Fig. 1 is a schematic diagram of an experimental setup in a lensless single-frame phase recovery method based on partially coherent LED illumination.
Fig. 2 is a flow chart of a lensless single-frame phase recovery method based on partially coherent LED illumination.
Fig. 3 is the captured single frame hologram, the reconstructed phase result directly back, and the final reconstructed phase resolution plate result.
FIG. 4 is the long-term live cell phase results reconstructed using the present invention and the digitized multi-modal results achieved without any external device.
Detailed Description
As shown in fig. 2, a lensless single-frame phase recovery method based on partially coherent led illumination comprises the following four steps:
step 1, collecting a holographic image by using a lensless on-chip microscope system.
As shown in fig. 1, the present invention is based on a conventional lensless on-chip microscope architecture, comprising only a light source 1, a sample 2, and a sensor 3. The single-color patch LED lighting source 1 adopted by the invention (632 nm, the bandwidth is 23.95nm, and the area of the light source is 0.0091 mm) 2 ) The sample wafer is directly illuminated, and the spectral width of the light source is digitally divided into 20 quasi-monochromatic sub-wavelengths at intervals of 2nm for sample reconstruction by using the spectral width obtained by measurement and experimental verification. Sample 2 was imaged directly on an image sensor, and the system used a plate-level monochrome CMOS sensor 3 (pixel size 0.9 μm,5664 × 4256, jiangsu tea Intelligence Technology co.
The specific implementation process comprises the following steps: directly irradiating a sample by using a wide-spectrum LED incoherent light source, and simultaneously triggering a camera to record a holographic image I under a central wavelength ini 。
And 2, segmenting the LED wide-spectrum wavelength according to the spectrum prior, and initializing phase constraint information.
The specific implementation process comprises the following steps: the illumination wavelength λ is divided into N equally spaced quasi-monochromatic subwavelengths { λ ] according to the spectrum of the prior illuminating LED i ,i＝1,2,...,N}；
Using central wavelength lambda c A light intensity chart I to be shot ini Combining the initial zero phase psi 0 =0 generating camera plane complex amplitude
Figure BDA0003843885530000051
Propagating the complex amplitude back to the object plane and then usingComplex amplitude of object plane
Figure BDA0003843885530000052
The phase part of (1) obtains an initial phase constraint Mask 0 Initial constraint Mask 0 Dynamically updating Mask in subsequent iteration process i 。
And 3, updating the complex amplitude of the iterative object by using the digitally-divided sub-wavelengths and the shot hologram, wherein the specific implementation process comprises the following steps:
step 3.1, apply hologram I ini Combined with last wavelength of the splitter i1 The phase information propagated back to the camera plane generates a camera plane complex amplitude with a specific formula:
Figure BDA0003843885530000053
in the formula (I), the compound is shown in the specification,
Figure BDA0003843885530000054
is the complex amplitude of the camera plane, omega i Is the weight of the ith sub-wavelength, j is the imaginary unit, ψ i For the last division sub-wavelength lambda i1 The phase information (initially 0) is propagated back to the camera plane.
Step 3.2, the complex amplitude is transmitted to the surface of the object by corresponding wavelength to obtain object surface complex amplitude, and the specific formula is as follows:
Figure BDA0003843885530000055
wherein H (-d) is a transfer function of the propagation distance-d,
Figure BDA0003843885530000056
is the complex amplitude of the object plane and,
Figure BDA0003843885530000057
for more than one sub-wavelength λ of division i-1 Propagating back to the mode of the complex amplitude behind the object plane,
Figure BDA0003843885530000058
is the object plane phase.
Step 3.3, applying phase support constraint to phase part of object plane complex amplitude
Figure BDA0003843885530000061
And updating the phase according to the wavelength
Figure BDA0003843885530000062
While applying uniform light intensity constraint to the amplitude component
Figure BDA0003843885530000063
Wherein ave (-) is a mean calculation operator to obtain an updated complex amplitude
Figure BDA0003843885530000064
Step 3.4, the updated object plane complex amplitude
Figure BDA0003843885530000065
Propagating back to the camera plane to obtain a split sub-wavelength λ i The phase information propagated back to the camera plane returns to step 3.1, incorporating the next sub-wavelength λ i+1 The weights update the camera face complex amplitude until all the split sub-wavelengths are traversed, thereby obtaining the high resolution and high signal to noise ratio phase ψ for the sample.
FIG. 3 shows the holographic pattern photographed and shows a 1.41 times resolution improvement of the present invention (0.977) compared to the resolution of the conventional single frame LED method (1.381 μm). FIG. 4 is a phase contrast, differential phase contrast and three-dimensional structural image reconstructed using the dynamic results of live cells cultured over an extended period of time and a digital method reconstructed using the present invention.
The method only needs to obtain a coaxial hologram through the illumination wavelength of the patch wide-spectrum LED under the vertical illumination of a light source, then uses a multi-wavelength-based phase recovery reconstruction algorithm and a dynamic phase support constraint method to combine a propagation model and a non-coherent superposition model to continuously propagate and update the intensity image on a camera plane and an object plane under different segmentation sub-wavelengths, and gradually recovers the phase information of a sample. The invention only has one surface-mounted LED light source, and does not introduce mechanical displacement, thereby improving the stability of the system and greatly saving the cost.

Claims (6)

1. A lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination is characterized by comprising the following steps:
step 1, collecting a holographic image of a sample;
step 2, splitting the LED wide-spectrum wavelength according to spectrum prior, and initializing phase constraint information;
and 3, updating the complex amplitude of the iterative object by using the sub-wavelength of the digital segmentation and the shot hologram to obtain the object phase, wherein the method specifically comprises the following steps:
transmitting an original intensity image to a focus plane by using sub-wavelengths of a wide-spectrum LED to obtain object plane complex amplitude, and respectively implementing phase support constraint and uniform light intensity constraint on the phase and the amplitude of the complex amplitude;
and transmitting the updated complex amplitude back to the surface of the camera by utilizing an angular spectrum theory to obtain phase information of the current segmentation sub-wavelength transmitted back to the plane of the camera, updating the amplitude information of the surface of the camera according to the shot hologram and the spectrum prior weight coefficient, traversing all the sub-wavelengths and recovering the phase information.
2. The method of claim 1, wherein the holographic image is acquired using a lensless on-chip microscopy system comprising a single LED illumination source and a sensor, wherein the LED illumination source is a broad-spectrum, not completely monochromatic, patch LED illumination source.
3. The lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination according to claim 1, wherein the step 2 is specifically:
dividing the illumination wavelength lambda into N equally spaced quasi-monochromators according to the spectrum of the prior illumination LEDWavelength { lambda i ,i＝1,2,...,N}；
Using central wavelength lambda c A light intensity map I to be photographed ini Combining the initial zero phase psi 0 =0 generating camera plane complex amplitude
Figure FDA0003843885520000011
Propagating the complex amplitude back to the object plane and then using the complex amplitude of the object plane
Figure FDA0003843885520000012
The phase part of (1) obtains an initial phase constraint Mask 0 。
4. The lens-free single-frame phase recovery method based on partially coherent LED illumination of claim 1, wherein the object phase is obtained by updating the iterative object complex amplitude with the digitally split sub-wavelengths and the captured hologram by:
step 3.1, hologram I ini Combined with last wavelength of the splitter i-1 Phase information propagated back to the camera plane generates a camera plane complex amplitude;
step 3.2, transmitting the complex amplitude to the surface of the object by using the corresponding wavelength to obtain object surface complex amplitude;
step 3.3, applying phase support constraint to phase part of object plane complex amplitude
Figure FDA0003843885520000021
And updating the phase according to the wavelength
Figure FDA0003843885520000022
While applying uniform light intensity constraints to the amplitude portion
Figure FDA0003843885520000023
Wherein ave (-) is a mean calculation operator to obtain an updated complex amplitude
Figure FDA0003843885520000024
Step 3.4, the updated object plane complex amplitude
Figure FDA0003843885520000025
Propagating back to the camera plane to obtain a split sub-wavelength λ i The phase information propagated back to the camera plane returns to step 3.1, incorporating the next sub-wavelength λ i+1 The weights update the camera face complex amplitude until all the split sub-wavelengths are traversed, thereby obtaining the high resolution and high signal-to-noise ratio phase ψ of the sample.
5. The method of claim 4, wherein the camera face complex amplitude generated in step 3.1 is specifically:
Figure FDA0003843885520000026
in the formula (I), the compound is shown in the specification,
Figure FDA0003843885520000027
is the complex amplitude of the camera plane, omega i Is the weight of the ith sub-wavelength, j is the imaginary unit, # i For the last division sub-wavelength lambda i-1 Phase information propagated back to the camera plane.
6. The lens-free single-frame phase recovery method based on partially coherent LED illumination according to claim 4, wherein the object plane complex amplitude obtained in step 3.2 is specifically:
Figure FDA0003843885520000028
wherein H (-d) is a transfer function of the propagation distance-d,
Figure FDA0003843885520000029
is the complex amplitude of the object plane and,
Figure FDA00038438855200000210
for more than one sub-wavelength λ of division i-1 Propagating back to the mode of the complex amplitude behind the object plane,
Figure FDA00038438855200000211
is the object plane phase.
CN202211110573.0A 2022-09-13 2022-09-13 Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination Pending CN115452743A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202211110573.0A CN115452743A (en) 2022-09-13 2022-09-13 Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination
PCT/CN2023/092135 WO2024055602A1 (en) 2022-09-13 2023-05-05 Lens-free single-frame phase recovery method based on partially coherent light-emitting diode illumination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211110573.0A CN115452743A (en) 2022-09-13 2022-09-13 Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination

Publications (1)

Publication Number Publication Date
CN115452743A true CN115452743A (en) 2022-12-09

Family

ID=84302413

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211110573.0A Pending CN115452743A (en) 2022-09-13 2022-09-13 Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination

Country Status (2)

Country Link
CN (1) CN115452743A (en)
WO (1) WO2024055602A1 (en)

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9581961B2 (en) * 2012-10-05 2017-02-28 University Of Hyogo Holographic microscope, holographic image generation method, and method for acquiring data for holographic image
EP3175302B1 (en) * 2014-08-01 2021-12-29 The Regents of the University of California Device and method for iterative phase recovery based on pixel super-resolved on-chip holography
CN104537611A (en) * 2014-10-28 2015-04-22 南京理工大学 Optimized light intensity transmission phase recovery method based on Savitzky-Golay differential filters
CN105806250B (en) * 2016-03-10 2018-04-17 中国科学院上海光学精密机械研究所 High-resolution three-dimension phase microscopic imaging device and imaging method
CN107576633B (en) * 2017-08-10 2020-10-02 南京理工大学 Method for detecting internal defects of optical element by using improved 3PIE technology
CN110470245B (en) * 2019-08-21 2021-02-26 浙江大学 Phase recovery detection device and phase recovery method based on Fresnel zone plate diffraction information fusion
CN111610623B (en) * 2020-04-29 2022-05-27 南京理工大学 Fourier stack-based microscopic imaging method
CN112255776B (en) * 2020-11-10 2022-08-02 四川欧瑞特光电科技有限公司 Point light source scanning illumination method and detection device
CN113393472B (en) * 2021-05-29 2022-08-16 南京理工大学 Zoning composite phase unwrapping method based on digital holographic microscopic imaging
CN114660060A (en) * 2022-03-07 2022-06-24 南京理工大学 Macroscopic Fourier laminated super-resolution imaging method based on matrix scanning
CN115144371A (en) * 2022-07-19 2022-10-04 南京理工大学 Lensless Fourier laminated diffraction tomography microscopic imaging method based on wavelength scanning

Also Published As

Publication number Publication date
WO2024055602A1 (en) 2024-03-21

Similar Documents

Publication Publication Date Title
US11422503B2 (en) Device and method for iterative phase recovery based on pixel super-resolved on-chip holography
Zuo et al. Wide-field high-resolution 3D microscopy with Fourier ptychographic diffraction tomography
Luo et al. Synthetic aperture-based on-chip microscopy
CN109580457B (en) Three-dimensional diffraction chromatography microscopic imaging method based on LED array coding illumination
Sibarita Deconvolution microscopy
Guo et al. Fourier ptychography for brightfield, phase, darkfield, reflective, multi-slice, and fluorescence imaging
Tseng et al. Lensfree microscopy on a cellphone
Chen et al. Superresolution structured illumination microscopy reconstruction algorithms: a review
US20120218379A1 (en) Incoherent lensfree cell holography and microscopy on a chip
Zheng et al. Fourier ptychographic microscopy: a gigapixel superscope for biomedicine
WO2024016774A1 (en) Wavelength-scanning-based lensless fourier ptychographic diffraction tomography microimaging method
KR20210155397A (en) System and method for deep learning-based color holographic microscopy
WO2023221741A1 (en) Transport of intensity diffraction tomography microscopic imaging method based on non-interferometric synthetic aperture
CN113671682B (en) Frequency domain light source position accurate correction method based on Fourier laminated microscopic imaging
Konda et al. Multi-aperture Fourier ptychographic microscopy, theory and validation
Fixler et al. Pattern projection for subpixel resolved imaging in microscopy
Tayebi et al. Real-time triple field of view interferometry for scan-free monitoring of multiple objects
CN110989155B (en) Lens-free microscopic imaging device based on optical filter array and reconstruction method
CN115452743A (en) Lens-free single-frame phase recovery method based on partially coherent light emitting diode illumination
CN114184553B (en) Annular light illumination-based epitype quantitative phase microscopic device and method
Ma et al. Light-field tomographic fluorescence lifetime imaging microscopy
US11422355B2 (en) Method and system for acquisition of fluorescence images of live-cell biological samples
US20230184683A1 (en) System and method for three-dimensional imaging of unstained samples using bright field microscopy
Peng et al. A Compact Real-time Quantitative Phase Imaging System
CN115602318B (en) Microscopic imaging method based on chromatic dispersion and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination