CN115445461A - Double-layer micro-droplet generation device and double-layer micro-droplet generation method - Google Patents
Double-layer micro-droplet generation device and double-layer micro-droplet generation method Download PDFInfo
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- CN115445461A CN115445461A CN202210987315.4A CN202210987315A CN115445461A CN 115445461 A CN115445461 A CN 115445461A CN 202210987315 A CN202210987315 A CN 202210987315A CN 115445461 A CN115445461 A CN 115445461A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/41—Emulsifying
- B01F23/414—Emulsifying characterised by the internal structure of the emulsion
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/41—Emulsifying
- B01F23/4105—Methods of emulsifying
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/41—Emulsifying
- B01F23/414—Emulsifying characterised by the internal structure of the emulsion
- B01F23/4145—Emulsions of oils, e.g. fuel, and water
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/45—Mixing liquids with liquids; Emulsifying using flow mixing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/40—Static mixers
- B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
- B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
Abstract
The invention discloses a double-layer micro-droplet generating device and a double-layer micro-droplet generating method, wherein the double-layer micro-droplet generating device comprises an inner phase sampling tube and a middle phase sampling tube, wherein the inner phase sampling tube and the middle phase sampling tube respectively extend into a first droplet generating tube to form a layer of micro-droplets; an external phase sampling tube; at least part of the outer phase sampling tube and at least part of the first droplet generation tube respectively extend into the second droplet generation tube to form double-layer micro droplets, and the inner phase sampling tube, the first droplet generation tube and the second droplet generation tube are all made of high molecular polymer materials. The extensible property of the two liquid drop generation pipelines of high molecular polymers is utilized, the inner diameter and the wall thickness of the two liquid drop generation pipelines can be easily changed under the condition of heating or applying external force, the adjustment of the size of the liquid drop is realized, a layer of micro liquid drop is firstly formed through the inner phase sampling pipe and the middle phase sampling pipe, then the first liquid drop generation pipe and the outer phase sampling pipe generate double-layer micro liquid drop, and the effect of quickly, stably and massively preparing the double-layer micro liquid drop with good uniformity is realized.
Description
Technical Field
The invention relates to the technical field of micro-droplets, in particular to a double-layer micro-droplet generation device and a double-layer micro-droplet generation method.
Background
The double-layer micro-droplets are complex droplets with more small droplets wrapped in dispersed phase droplets, and the outer droplets form a shielding layer around the inner droplets, so that the inner droplets and the continuous phase can be effectively isolated. Double-layer microdroplets, which have more and more flexible structural features than single emulsion droplets, have attracted more and more research interest to scientists due to their unique core-shell structure and material selectivity. The double-layer micro-droplets are widely applied to the fields of cells, biochemistry, catalysts, self-healing materials, carbonless copy paper, electronic ink, carbon dioxide adsorption and the like. The traditional method for preparing the double-layer micro-droplets is mainly a mechanical stirring method, the prepared micro-droplets are poor in uniformity and low in micro-wrapping success rate, and the subsequent application range is limited.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention provides a double-layer micro-droplet generation device and a double-layer micro-droplet generation method, which have the advantages of capability of quickly preparing a large number of double-layer micro-droplets with good uniformity and high micro-wrapping success rate.
A double-layer microdroplet generation device according to some embodiments of the present invention comprises:
the inner phase sampling tube is made of high molecular polymer material;
a middle phase sampling pipe;
the first liquid drop generating tube is made of high molecular polymer materials, at least part of the inner phase sampling tube and at least part of the middle phase sampling tube respectively extend into the first liquid drop generating tube from one side of the first liquid drop generating tube, and the inner phase sampling tube and the middle phase sampling tube are arranged at intervals;
an external phase sampling pipe;
the second liquid drop generating pipe is made of high polymer materials, at least part of the external phase sampling pipe and at least part of the other side of the first liquid drop generating pipe extend into the second liquid drop generating pipe respectively, and the external phase sampling pipe and the first liquid drop generating pipe are arranged at intervals.
The double-layer micro-droplet generating device at least has the following beneficial effects: the inner diameter and the wall thickness of the two liquid drop generating pipelines and the inner phase sampling pipe of high molecular polymers can be easily changed under the condition of heating or applying external force, so that the size of the liquid drops can be adjusted, a layer of micro liquid drops are formed through the inner phase sampling pipe and the middle phase sampling pipe, and then double-layer micro liquid drops are generated through the first liquid drop generating pipe and the outer phase sampling pipe, so that the effect of quickly, stably and massively preparing the double-layer micro liquid drops with good uniformity is realized.
According to some embodiments of the invention, the first droplet generation tube has a first side portion and a second side portion disposed opposite the first side portion, the first side portion tapering toward the second side portion.
According to some embodiments of the invention, at least part of the inner phase sample tube and at least part of the middle phase sample tube each extend into the first droplet generation tube from a first side portion of the first droplet generation tube.
According to some embodiments of the invention, the first droplet generation tube has a fifth end portion between the first side portion and the second side portion, the first side portion extends to form a third chamber, and at least a portion of the middle phase sampling tube extends into the third chamber.
According to some embodiments of the invention, the fifth end portion is directed to the second side portion to form a fifth chamber, at least a portion of the inner phase sample tube extends to the third chamber and the fifth chamber in sequence, and an end of the inner phase sample tube near the second side portion is spaced apart from the second side portion.
According to some embodiments of the invention, the second droplet generation tube comprises a first end portion, a second end portion and a third end portion in sequence from one side to the opposite side, the first end portion gradually narrows towards the second end portion, and the third end portion gradually narrows towards the second end portion.
According to some embodiments of the invention, the first end extends towards the second end to form a first chamber, the third end extends towards the second end to form a second chamber, and at least part of the external phase sampling tube and at least part of the other side of the first droplet generation tube protrude into the first chamber or the second chamber, respectively.
According to some embodiments of the invention, the first droplet generation tube extends into the second droplet generation tube to a depth d1, the external sampling tube extends into the second droplet generation tube to a depth d2, and d1 > d2.
In the double-layer micro-droplet generation method according to another embodiment of the present invention, a double-layer micro-droplet generation device is used to generate a double-layer micro-droplet;
wherein, double-deck little liquid droplet generating device includes:
the inner phase sampling tube is made of high molecular polymer material;
a middle phase sampling pipe;
the first liquid drop generating pipe is made of high polymer materials, at least part of the inner phase sampling pipe and at least part of the middle phase sampling pipe respectively extend into the first liquid drop generating pipe from one side of the first liquid drop generating pipe, and the inner phase sampling pipe and the middle phase sampling pipe are arranged at intervals;
an external phase sampling pipe;
the second liquid drop generating tube is made of high molecular polymer materials, at least part of the external phase sampling tube and at least part of the other side of the first liquid drop generating tube extend into the second liquid drop generating tube respectively, and the external phase sampling tube and the first liquid drop generating tube are arranged at intervals;
the double-layer micro-droplet generation method comprises the following steps:
1) A first liquid is introduced into the inner phase sample introduction pipe, a second liquid is introduced into the middle phase sample introduction pipe, and the first liquid and the second liquid form a layer of micro liquid drops in the first liquid drop generation pipe;
2) And introducing a third liquid into the external phase sample inlet pipe, wherein the third liquid and the layer of micro-droplets form a double-layer micro-droplet in the second droplet generation pipe.
According to some embodiments of the invention, the first body fluid is an aqueous phase, the second liquid is an oil phase such that the one layer of microdroplets formed is water-in-oil microdroplets, the third liquid is an aqueous phase, the first body fluid and the second liquid being of different composition such that the bilayer microdroplets formed are water-in-oil-in-water microdroplets;
or the first body fluid is an oil phase, the second liquid is a water phase, so that the formed layer of micro droplets is oil-in-water micro droplets, the third liquid is an oil phase, and the first body fluid and the second liquid have different components, so that the formed double-layer micro droplets are oil-in-water-in-oil micro droplets.
The double-layer micro-droplet generation method at least has the following beneficial effects: the extensible property of the two liquid drop generating pipelines of high molecular polymers and the extensible property of the inner phase sample injection pipe are utilized, the inner diameter and the wall thickness of the liquid drop generating pipelines can be easily changed under the condition of heating or applying external force, the adjustment of the size of the liquid drop is realized, a layer of micro liquid drop is firstly formed by the inner phase sample injection pipe and the middle phase sample injection pipe, then the double-layer micro liquid drop is generated by the first liquid drop generating pipe and the outer phase sample injection pipe, and the effect of quickly, stably and massively preparing the double-layer micro liquid drop with good uniformity is realized.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The invention is further described in the following with reference to the accompanying drawings, it is obvious that the drawings in the following description are some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from these drawings without inventive effort.
FIG. 1 is a schematic perspective view of an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of FIG. 1;
FIG. 3 is a schematic cross-sectional view of a second droplet-generating tube according to an embodiment of the invention;
fig. 4 is a schematic sectional view of a first droplet generation tube according to an embodiment of the present invention.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present invention, embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
It is to be understood that, herein, if any terms such as "upper", "lower", "left", "right", "front", "rear", "longitudinal", "transverse", "axial", etc., indicate orientations or positional relationships based on those shown in the drawings, this is for convenience in describing and simplifying the present invention, and does not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered limiting of the present invention.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Herein, the terms "first", "second", "third", etc. are used for distinguishing different objects, and are not used for describing a particular order. As used herein, the terms "a", "an", and "the" are used interchangeably, and the term "a" and "an" are used interchangeably.
In the description herein, unless expressly defined otherwise, the terms set, mounted, connected, and the like are to be construed broadly, e.g., as meaning a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.
The double-layer micro-droplets are complex droplets with more small droplets wrapped in dispersed phase droplets, and the outer droplets form a shielding layer around the inner droplets, so that the inner droplets and the continuous phase can be effectively isolated. Double-layer microdroplets, which have more and more flexible structural features than single emulsion droplets, have attracted more and more research interest to scientists due to their unique core-shell structure and material selectivity. The double-layer micro-droplets are widely applied to the fields of cells, biochemistry, catalysts, self-healing materials, carbonless copy paper, electronic ink, carbon dioxide adsorption and the like. The traditional method for preparing the double-layer micro-droplets comprises a mechanical stirring method and a membrane emulsification method, the prepared micro-droplets have poor uniformity and low micro-wrapping success rate, and the subsequent application range is limited.
Referring to fig. 1 to 4, the present invention provides a double-layer micro-droplet generating device, which includes an inner phase sampling tube 100, a middle phase sampling tube 200, an outer phase sampling tube 300, a first droplet generating tube 400, and a second droplet generating tube 500.
Specifically, the inner phase sampling tube 100, the first droplet-generating tube 400, and the second droplet-generating tube 500 are all made of a high molecular weight polymer material.
It is understood that the inner phase sampling tube 100 is made of a polyetheretherketone material or a polytetrafluoroethylene material. The first droplet generating tube 400 is made of a peek material or a ptfe material. The second droplet-generating tube 500 is made of a polyetheretherketone material or a polytetrafluoroethylene material.
It should be understood that the inner phase sampling tube 100, the droplet generation tube, and the second droplet generation tube 500 may be replaced by microchannels made of other materials with stretching characteristics.
It is understood that the outer phase sample introduction tube 300 is a capillary tube made of stainless steel, and the middle phase sample introduction tube 200 is a capillary tube made of stainless steel. It should be understood that the outer phase sampling tube 300 and the middle phase sampling tube 200 may be made of other metal or non-metal materials.
With continued reference to fig. 1 and 2, at least a portion of the inner phase sampling tube 100 and at least a portion of the middle phase sampling tube 200 extend into the first droplet generation tube 400 from one side of the first droplet generation tube 400, respectively, with the inner phase sampling tube 100 and the middle phase sampling tube 200 being spaced apart.
At least a part of the external phase sampling tube 300 and at least a part of the other side of the first droplet generating tube 400 are inserted into the second droplet generating tube 500, respectively, and the external phase sampling tube 300 and the first droplet generating tube 400 are spaced apart from each other.
The double-layer micro-droplet generating device is a micro-fluidic device manufactured based on a micro-fluidic technology, and the micro-fluidic device for manufacturing the double-layer micro-droplets generally adopts the technologies of photoetching, 3D printing and the like or uses a glass capillary. The methods such as photolithography and 3D printing require expensive instruments and complex processes, and are high in cost, long in the periphery of the product, and limited in the types of materials used for manufacturing the device.
The micro-fluidic device prepared by the glass capillary has higher requirements on the processing precision of the glass capillary, the finished glass capillary is difficult to reprocess, and has the problems of fragility and damage, and is not favorable for carrying, transportation and large-scale production. These problems have hindered the development of wider applications of double-layer microdroplets.
According to the invention, by utilizing the extensibility of the two liquid drop generating pipelines of high molecular polymers and the extendibility of the inner phase sampling pipe 100, the inner diameter and the wall thickness of the liquid drop generating pipelines can be easily changed under the condition of heating or applying external force, so that the adjustment of the size of the liquid drop is realized, a layer of micro liquid drop is firstly formed by the inner phase sampling pipe 100 and the middle phase sampling pipe 200, and then the double-layer micro liquid drop is generated by the first liquid drop generating pipe 400 and the outer phase sampling pipe 300, so that the effect of quickly and stably preparing a large number of double-layer micro liquid drops with good uniformity is realized.
With continued reference to fig. 4, in some embodiments, the first droplet generation tube 400 has a first side portion 410 and a second side portion 420 disposed opposite the first side portion 410, the first side portion 410 tapering toward the second side portion 420.
With continued reference to fig. 2, it can be appreciated that at least a portion of the inner phase sample tube 100 and at least a portion of the middle phase sample tube 200 each extend from the first side 410 of the first drop generator tube 400 into the first drop generator tube 400.
With continued reference to fig. 2 and 4, it will be appreciated that the first drop generating tube 400 has a fourth end 430 between the first side 410 and the second side 420, the first side 410 extending toward the fourth end 430 to form a third chamber 440, and at least a portion of the middle sampling tube 200 extending into the third chamber 440.
It will be appreciated that the fourth end 430 is directed toward the second side 420 to form a fourth chamber 450, and at least a portion of the inner phase sample tube 100 extends into the third chamber 440 and the fourth chamber 450 in sequence, such that the fourth chamber 450 is located in the first chamber 540, and a distance 600 exists between an end of the inner phase sample tube 100 near the second side 420 and the second side 420.
With continued reference to FIG. 4, it will be appreciated that the third chamber 440 and the fourth chamber 450 each have a slope, with the slope 610 of the third chamber > the slope 620 of the fourth chamber.
With continued reference to FIG. 3, in some embodiments, second droplet-generating tube 500 includes, in order from one side to the opposite side, a first end 510, a second end 520, and a third end 530, where first end 510 tapers toward second end 520, and third end 530 tapers toward second end 520.
With continued reference to fig. 2 and 3, it can be appreciated that the first end 510 extends toward the second end 520 to form the first chamber 540, the third end 530 extends toward the second end 520 to form the second chamber 550, and at least a portion of the external phase feed tube 300 and at least a portion of the other side of the first droplet generation tube 400 extend into the first chamber 540 or the second chamber 550, respectively.
With continued reference to FIG. 2, it will be appreciated that the first drop generating tube 400 extends into the second drop generating tube 500 to a depth d1, and the external sampling tube 300 extends into the second drop generating tube 500 to a depth d2, d1 > d2.
It will be appreciated that the depth of the inner phase sample tube 100 extending into the first droplet generation tube 400 is d3 and the depth of the middle phase sample tube 200 extending into the first droplet generation tube 400 is d4, d3 > d4.
It is understood that the joints of the pipes in the double-layer micro-droplet generating device of the present invention can be sealed by hot melt adhesive, and can also be sealed by other adhesives to prevent liquid leakage.
The double-layer micro-droplet generating device is based on a high molecular polymer pipeline, and a simple generating device is manufactured. The double-layer micro-droplet generating device has the characteristics of low cost, convenience in carrying and transportation, simple process, short manufacturing period and the like. The pipe is processed and prepared by using high molecular polymers, and the wall thickness and the inner diameter of the pipe can be easily changed by heating or forcibly directly stretching, so that the inner diameter and the wall thickness with gradient are formed. According to the experimental requirements, different double-layer micro-droplet generation devices can be prepared by combining pipelines with different diameters. The glass capillary or expensive 3D printer which is processed with high precision is avoided, and the cost of the single double-layer micro-droplet generation device is reduced. Meanwhile, the limitation of the types of manufacturing materials is overcome. The high molecular polymer pipeline has the characteristics of better toughness, avoidance of the fragility of glass products, and contribution to carrying, transportation and large-scale production. The method can be assembled by only a few steps without using complex instruments, and the manufacturing period is short. The double-layer micro-droplet generating device can rapidly prepare a large number of double-layer micro-droplets with good uniformity.
In other embodiments of the present invention, a double-layer micro-droplet generating apparatus is provided, which is used to generate double-layer micro-droplets.
The double-layer micro-droplet generation method comprises the following steps:
1) A first liquid is introduced into the inner phase sampling tube 100, a second liquid is introduced into the middle phase sampling tube 200, and the first liquid and the second liquid form a layer of micro liquid drops in the first liquid drop generating tube 400;
2) The external phase sampling tube 300 is filled with a third liquid, and the third liquid and a layer of micro-droplets form a double-layer micro-droplet in the second droplet generation tube 500.
In some embodiments, the first body fluid is an aqueous phase, the second fluid is an oil phase such that a layer of microdroplets formed is water-in-oil microdroplets, the third fluid is an aqueous phase, the first body fluid and the second fluid are different in composition such that a bilayer of microdroplets formed is water-in-oil-in-water microdroplets;
in other embodiments, the first body fluid is an oil phase, the second fluid is an aqueous phase such that a layer of microdroplets formed is oil-in-water microdroplets, the third fluid is an oil phase, and the first body fluid and the second fluid are of different composition such that a bilayer of microdroplets formed is oil-in-water-in-oil microdroplets.
It will be appreciated that after the second droplet-generating tube 500 forms a double layer of microdroplets, the double layer of microdroplets may be collected with a container at the other end of the second droplet-generating tube 500.
In some embodiments, the liquid introduced into the inner phase sample introduction tube 100, the middle phase sample introduction tube 200 and the outer phase sample introduction tube 300 can be driven by a syringe pump, an air pump or other power sources for liquid transportation.
The micro-fluidic technology has the characteristics of precise control and micro-scale fluid flow treatment, and has the advantages of small volume, integration and high automation degree. The device manufactured based on the microfluidic technology can be used for preparing double-layer micro-droplets with good uniformity and high micro-wrapping success rate.
In the description of the specification, references to the description of "one embodiment," "some embodiments," "other embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts of the present invention. It should be noted that there are no specific structures but a few objective structures due to the limited character expressions, and that those skilled in the art may make various improvements, decorations or changes without departing from the principle of the invention or may combine the above technical features in a suitable manner; such modifications, variations, or combinations are intended to be included within the scope of the invention as defined in the claims and their equivalents.
Claims (10)
1. A double-layer microdroplet generating device, comprising:
the inner phase sampling tube is made of high molecular polymer material;
a middle phase sampling pipe;
the first liquid drop generating tube is made of high molecular polymer materials, at least part of the inner phase sampling tube and at least part of the middle phase sampling tube respectively extend into the first liquid drop generating tube from one side of the first liquid drop generating tube, and the inner phase sampling tube and the middle phase sampling tube are arranged at intervals;
an external phase sampling pipe;
the second droplet generation pipe is made of high polymer materials, at least part of the external phase sampling pipe and at least part of the other side of the first droplet generation pipe extend into the second droplet generation pipe respectively, and the external phase sampling pipe and the first droplet generation pipe are arranged at intervals.
2. The double-layer droplet generator according to claim 1, wherein the first droplet generator has a first side portion and a second side portion opposite to the first side portion, and the first side portion is tapered toward the second side portion.
3. The dual layer micro droplet generator of claim 2, wherein at least a portion of the inner phase sample tube and at least a portion of the middle phase sample tube each extend into the first droplet generation tube from a first side portion of the first droplet generation tube.
4. The dual layer droplet generator of claim 3, wherein the first droplet generator tube has a fifth end portion extending from the first side portion to the second side portion, the first side portion extending toward the fifth end portion to form a third chamber, and at least a portion of the middle phase sampling tube extends into the third chamber.
5. The double-layer droplet generator of claim 4, wherein the fifth end portion is directed to the second side portion to form a fifth chamber, at least a portion of the inner-phase sample tube extends to the third chamber and the fifth chamber in sequence, and a distance is provided between an end of the inner-phase sample tube near the second side portion and the second side portion.
6. The double-layer droplet generator according to claim 1, wherein the second droplet generator comprises a first end, a second end and a third end in sequence from one side to the opposite side, the first end is gradually narrowed toward the second end, and the third end is gradually narrowed toward the second end.
7. The dual layer droplet generator of claim 6, wherein the first end extends toward the second end to form a first chamber, the third end extends toward the second end to form a second chamber, and at least a portion of the external sample introduction tube and at least a portion of the other side of the first droplet generator tube extend into the first chamber or the second chamber, respectively.
8. The dual layer droplet generator of claim 7, wherein the first droplet generator tube extends into the second droplet generator tube to a depth d1, the external phase introduction tube extends into the second droplet generator tube to a depth d2, and d1 > d2.
9. The double-layer micro-droplet generation method is characterized in that a double-layer micro-droplet generation device is adopted to generate double-layer micro-droplets;
wherein, double-deck little liquid droplet generating device includes:
the inner phase sampling tube is made of high molecular polymer material;
a middle phase sampling pipe;
the first liquid drop generating pipe is made of high polymer materials, at least part of the inner phase sampling pipe and at least part of the middle phase sampling pipe respectively extend into the first liquid drop generating pipe from one side of the first liquid drop generating pipe, and the inner phase sampling pipe and the middle phase sampling pipe are arranged at intervals;
an external phase sampling pipe;
the second liquid drop generating tube is made of high molecular polymer materials, at least part of the external phase sampling tube and at least part of the other side of the first liquid drop generating tube extend into the second liquid drop generating tube respectively, and the external phase sampling tube and the first liquid drop generating tube are arranged at intervals;
the double-layer micro-droplet generation method comprises the following steps:
1) A first liquid is introduced into the inner phase sample introduction pipe, a second liquid is introduced into the middle phase sample introduction pipe, and the first liquid and the second liquid form a layer of micro liquid drops in the first liquid drop generation pipe;
2) And introducing a third liquid into the external phase sample inlet pipe, wherein the third liquid and the layer of micro-droplets form a double-layer micro-droplet in the second droplet generation pipe.
10. The double-layer microdroplet generation method of claim 9, characterized in that:
the first body fluid is an aqueous phase, the second liquid is an oil phase, so that the formed micro-droplets of the layer are water-in-oil micro-droplets, the third liquid is an aqueous phase, and the first body fluid and the second liquid have different components, so that the formed double-layer micro-droplets are water-in-oil-in-water micro-droplets;
or the first body fluid is an oil phase, the second liquid is a water phase, so that the formed layer of micro droplets is oil-in-water micro droplets, the third liquid is an oil phase, and the first body fluid and the second liquid have different components, so that the formed double-layer micro droplets are oil-in-water-in-oil micro droplets.
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