CN115429703B - Dual gel composition and method for preparing the same - Google Patents
Dual gel composition and method for preparing the same Download PDFInfo
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- CN115429703B CN115429703B CN202211302982.0A CN202211302982A CN115429703B CN 115429703 B CN115429703 B CN 115429703B CN 202211302982 A CN202211302982 A CN 202211302982A CN 115429703 B CN115429703 B CN 115429703B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Abstract
The invention relates to a double gel composition and a preparation method thereof, wherein the double gel composition comprises, by weight, 0.12-0.25% of plant polyphenol, 32.5-63.7% of grease, 0.75-2.9% of oil phase gel, 0.15-1.2% of water phase gel, 0.2-0.61% of auxiliary agent and the balance of water, wherein the plant polyphenol comprises at least one of mangiferin, naringin and naringenin. The special plant polyphenol is embedded in the double gel system, so that the coating capacity and stability of the plant polyphenol in the double gel system can be improved, the preparation method is simple, the quality of the obtained product is stable, the product can be used as an external skin care product, and the product has the effects of moisturizing skin and assisting in improving skin inflammation.
Description
Technical Field
The present disclosure relates to the technical field of daily chemicals, and in particular, to a dual gel composition and a preparation method thereof.
Background
The double gel system is one gel system comprising oil gel and hydrogel mixed in certain proportion. Therefore, the gel has the advantages of two gel systems, namely hydrophilic and lipophilic substances can be delivered simultaneously, and the gel has good extensibility, moisture retention, skin permeability and stability. In addition, compared with other biphasic systems such as emulsion, the oil gel and the hydrogel are semi-solid, so that the two-gel system has better thermodynamic and kinetic stability after being structured, and the retention rate of bioactive substances can be effectively improved, so that the two-gel system is widely applied to medical treatment, chemical industry, cosmetics and food industry.
Plant polyphenol is an important plant secondary metabolite, and has good biological activity due to a plurality of phenolic hydroxyl groups in the molecular structure, such as anti-inflammatory, diabetes preventing, anti-tumor, anticancer, immunoregulation, antibacterial, antioxidant, intestinal flora regulating and the like. In addition, plant polyphenols have a potential therapeutic effect on skin inflammation, mainly due to the antioxidant capacity of polyphenols. Inflammation is a defensive reaction generated by the body when the body is stimulated to cause injury, a large amount of free radicals are generated in the body when the inflammation occurs, biochemical reaction is promoted, and a large amount of inflammatory factors are generated. The polyphenol can effectively remove free radicals and metabolites thereof, inhibit enzyme activity of enzymes related to inflammation and expression of inflammatory factors, thereby reducing generation and conduction of inflammatory signals and further improving inflammation. However, the disadvantages of poor chemical stability (sensitivity to oxygen, light, pH, temperature, etc.) and low absorption rate of polyphenols have greatly limited the use and efficacy of polyphenols.
The plant polyphenol double gel system has to be further studied in the aspects of moisturizing skin and improving skin inflammation in an auxiliary way in the prior art.
Disclosure of Invention
The object of the present disclosure is to provide a plant polyphenol-containing dual gel composition having the effects of moisturizing skin and aiding in improving skin inflammation, and a preparation method thereof.
In order to achieve the above object, a first aspect of the present disclosure provides a dual gel composition, which is characterized in that the dual gel composition comprises, by weight, 0.12 to 0.25% of plant polyphenol, 32.5 to 63.7% of grease, 0.75 to 2.9% of oil phase gelling agent, 0.15 to 1.2% of aqueous phase gelling agent, 0.2 to 0.61% of auxiliary agent, and the balance of water, wherein the plant polyphenol comprises at least one of mangiferin, naringin and naringenin.
Optionally, the double gel composition comprises, by weight, 0.18-0.22% of plant polyphenol, 38-48.5% of grease, 1.05-2.0% of oil phase gel, 0.38-0.95% of water phase gel, 0.35-0.47% of auxiliary agent, and the balance of water.
Optionally, the plant polyphenol is mangiferin, naringin and naringenin, and the weight ratio of mangiferin, naringin and naringenin is 1: (0.2-2) and (0.8-3).
Optionally, the weight ratio of mangiferin to naringin is 1: (0.5-1.2) and (1-1.5).
Optionally, the oil is one or more selected from jojoba oil, shea butter and glycerin.
Optionally, the oil phase gelling agent is candelilla wax and/or beeswax.
Optionally, the aqueous phase gel is one or more selected from locust bean gum, flaxseed gum and beta-glucan.
Optionally, the auxiliary agent comprises a preservative and a pH regulator, wherein the content of the preservative is 0.01-0.21% by weight, and the content of the pH regulator is 0.23-0.40% by weight.
Optionally, the preservative is pinacol and/or phenoxyethanol; the pH regulator is sodium hydroxide and/or hydrochloric acid.
In a second aspect of the present disclosure, there is provided a method of preparing the dual gel composition, the method comprising:
mixing the oil phase gel with the grease, and stirring at 67-75 ℃ to dissolve the grease to obtain oil gel;
mixing the aqueous phase gel with a first pH regulator, and stirring at 78-85 ℃ to dissolve the aqueous phase gel to obtain hydrogel;
mixing the plant polyphenol with a second pH regulator, and dissolving the plant polyphenol under the conditions of ultrasonic power of 45-85W and time of 25-60 s to obtain a plant polyphenol aqueous solution;
mixing the hydrogel with the plant polyphenol aqueous solution according to the weight ratio of (1-2) to 1, and stirring for 1-3 min under the condition of 10000-18000 rpm of a high-speed shearing machine to obtain the hydrogel containing plant polyphenol;
mixing the hydrogel containing plant polyphenol with the oleogel according to the weight ratio of (0.5-2): 1, adding the auxiliary agent, controlling the pH value range to be 4.0-6.0, homogenizing in a vacuum emulsifying machine, and setting parameters of the vacuum emulsifying machine: vacuum degree-0.097 MPa, temperature 52-82 ℃, homogenizing speed 10000-18000 rpm, homogenizing time 2-5 min, and obtaining the double gel composition.
Through the technical scheme, the special plant polyphenol is embedded in the double gel system, the coating capacity and stability of the plant polyphenol in the double gel system can be improved, the preparation method is simple, the quality of the obtained product is stable, the product can be used as an external skin care product, and the product has the effects of moisturizing skin and assisting in improving skin inflammation.
Additional features and advantages of the present disclosure will be set forth in the detailed description which follows.
Drawings
The accompanying drawings are included to provide a further understanding of the disclosure, and are incorporated in and constitute a part of this specification, illustrate the disclosure and together with the description serve to explain, but do not limit the disclosure. In the drawings:
FIG. 1 is a diagram showing the appearance of the double-set compositions produced in examples 1 to 9, and examples 1 to 9 are shown in the order from left to right.
FIG. 2 is a graph showing retention of plant polyphenol during storage of the dual gel composition prepared in example 3, wherein the abscissa represents time and the ordinate represents retention of plant polyphenol.
FIG. 3 is a graph showing the centrifugal stability of the dual gel compositions prepared in examples 1 to 5, and examples 1 to 5 are shown in the order from left to right.
FIG. 4 is a graph showing the appearance of the ears of mice for the first behavior test and a graph showing the tissue sections of the ears of mice for the second behavior test, showing the results of the anti-dermatitis evaluation of ears of mice for the dual gel compositions prepared in examples 2 and 6 to 9.
Detailed Description
Specific embodiments of the present disclosure are described in detail below with reference to the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the disclosure, are not intended to limit the disclosure.
In a first aspect of the present disclosure, a dual gel composition is provided, which comprises, in weight percentage, 0.12 to 0.25% of plant polyphenol, 32.5 to 63.7% of grease, 0.75 to 2.9% of oil phase gelling agent, 0.15 to 1.2% of aqueous phase gelling agent, 0.2 to 0.61% of auxiliary agent, and the balance water, wherein the plant polyphenol comprises at least one of mangiferin, naringin and naringenin.
The inventors of the present disclosure found in a great deal of research that in the construction of a double gel composition containing plant polyphenol, the selection of oil phase gellant, aqueous phase gellant, oil gel to hydrogel ratio, and plant polyphenol kind and addition amount has an important influence on the formation and stability of the final double gel composition. In terms of the dosage of the gel, the gel has high dosage, hard texture and poor spreadability; the gel agent has low dosage, soft texture and poor stability. In the aspect of the proportion of the oleogel to the hydrogel, the oleogel has high proportion, good adhesiveness but heavy greasy feeling; the hydrogel has high proportion, good skin feel and good water retention, but is unfavorable for dissolving and absorbing lipophilic polyphenol. In terms of the types and the addition amounts of the plant polyphenols, the effects of different plant polyphenols are different, and if the addition modes are improper and the addition amounts are too high, the plant polyphenols are easy to disperse unevenly or crystallize and separate out, so that the sensory and efficacy of the product are affected. The double gel composition has proper component selection, dosage and proportion, stable product quality, can be used as an external skin care product, and has the effects of moisturizing skin and assisting in improving skin inflammation.
In a preferred embodiment of the present disclosure, the dual gel composition comprises, in weight percent, 0.18 to 0.22% of plant polyphenol, 38 to 48.5% of grease, 1.05 to 2.0% of oil phase gelling agent, 0.38 to 0.95% of aqueous phase gelling agent, 0.35 to 0.47% of auxiliary agent, and the balance of water. The double gel composition in the above amount range has better stability and more excellent quality.
Wherein the plant polyphenol comprises Mangiferin with chemical formula of C 19 H 18 O 11 CAS number 4773-96-0; the Naringin has the English name Naringin and chemical molecular formula of C 27 H 32 O 14 CAS number 10236-47-2; the Naringenin has an English name of Naringenin and a chemical molecular formula of C 15 H 12 O 5 The CAS number is 67604-48-2. The plant polyphenols described above are all commercially available.
In a preferred embodiment of the present disclosure, the plant polyphenols are mangiferin, naringin and naringenin, and the plant polyphenols thus combined have a good synergistic effect for use in a dual gel composition. Further, the weight ratio of mangiferin, naringin and naringenin may be 1: (0.2-2): (0.8-3), preferably 1: (0.5-1.2) and (1-1.5).
In a preferred embodiment of the present disclosure, the grease may be one or more selected from jojoba oil, shea butter and glycerin. The oil phase gelling agent may be candelilla wax and/or beeswax. The aqueous phase gel may be one or more selected from locust bean gum, flaxseed gum and beta-glucan. The above materials are all commercially available.
In preferred embodiments of the present disclosure, the delivered auxiliary agent may include a preservative and a pH adjuster. The preservative is used for preventing microbial infection and proliferation and prolonging the preservation period of the double gel composition. The pH regulator is used for regulating the pH value of the double gel composition to a proper range, such as 4-6, and the pH value range can effectively prevent degradation of plant polyphenol. Further, the preservative may be present in an amount of 0.01 to 0.21% by weight; the content of the pH adjuster may be adjusted as required as long as the above-mentioned requirements can be satisfied, and for example, may be 0.23 to 0.40%. Further, the preservative may be pinacol and/or phenoxyethanol. The pH adjuster may be sodium hydroxide and/or hydrochloric acid.
In a second aspect of the present disclosure, there is provided a method of preparing the dual gel composition, the method comprising:
mixing the oil phase gel with the grease, and stirring at 67-75 ℃ to dissolve the grease to obtain oil gel;
mixing the aqueous phase gel with a first pH regulator (for example, hydrochloric acid solution with the concentration of 0.1mol/L, for example) and stirring at 78-85 ℃ to dissolve the aqueous phase gel to obtain hydrogel;
mixing the plant polyphenol with a second pH regulator (for example, sodium hydroxide solution with the concentration of 0.1mmol/L, for example), and dissolving the plant polyphenol under the conditions of ultrasonic power of 45-85W and time of 25-60 s to obtain a plant polyphenol aqueous solution;
mixing the hydrogel with the plant polyphenol aqueous solution according to the weight ratio of (1-2) to 1, and stirring for 1-3 min under the condition of 10000-18000 rpm of a high-speed shearing machine to obtain the hydrogel containing plant polyphenol;
mixing the plant polyphenol-containing hydrogel with the oleogel according to the weight ratio of (0.5-2): 1, adding the auxiliary agent (for example, hydrochloric acid can be used for adjusting the pH value to 4.0-6.0, and a preservative is added), and homogenizing in a vacuum emulsifying machine, wherein the vacuum emulsifying machine is provided with the parameters: vacuum degree-0.097 MPa, temperature 52-82 ℃, homogenizing speed 10000-18000 rpm, homogenizing time 2-5 min, and obtaining the double gel composition.
Further, the total preparation time from the aqueous plant polyphenol solution to the step of preparing the dual gel composition is completed within 15 to 30 minutes, which is advantageous for maintaining the stability of plant polyphenol.
Further, the method may further include: the prepared dual gel composition is canned into a packaging bottle and cooled to room temperature at 4-25 ℃.
The double gel composition prepared by the method is in a gel state, has certain spreadability, has no fluidity (no liquid flow after inversion), has uniform double gel paste distribution, and has no obvious layering, milk precipitation and other phenomena.
The present disclosure is further described below in connection with the following examples, but the present disclosure is not limited to the scope of the following examples.
Mangiferin used in the following examples was purchased from Shanghai Ala Biochemical technologies Co., ltd, commercial number M196329-25g; naringin is purchased from Shanghai Micalin Biochemical technologies Co., ltd, with a commercial number of N4677-50g; naringenin was purchased from Shanghai Meilin Biochemical technologies Co., ltd under the trade designation N859366-5g. The other raw materials are all commercial products.
Example 1
(1) Accurately weighing 2.0g of candelilla wax, adding into 38g of glycerin, and stirring at 70deg.C to dissolve to obtain oleogel;
(2) Accurately weighing 0.56g of flaxseed gum, adding into 29.4g of 0.1mol/L hydrochloric acid solution, stirring at 85 ℃ to dissolve the flaxseed gum to obtain hydrogel;
(3) Accurately weighing 0.18g of plant polyphenol (wherein mangiferin 0.06g, naringin 0.06g and naringenin 0.06 g) and dissolving in 29.7g of 0.1mol/L sodium hydroxide solution, wherein ultrasonic power is 45W, and 60s assist in dissolving the plant polyphenol composition to obtain a plant polyphenol composition aqueous solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 1:1, stirring for 3min at 10000rpm of a high-speed shearing machine to obtain hydrogel containing plant polyphenol, adding the obtained hydrogel containing plant polyphenol and the oil gel prepared in the step (1) into a vacuum emulsifying machine according to a weight ratio of 1.5:1, adding hydrochloric acid to adjust the pH value to 6.0, and then adding 0.1g of phenoxyethanol; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 82 deg.C, homogenizing speed 10000rpm, homogenizing time 5min, and making into double gel composition.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 30min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 25 ℃ to obtain the double gel paste containing plant polyphenol.
The composition of the dual gel product prepared in this example was: 0.18 wt% of plant polyphenol, 38 wt% of grease, 2.0 wt% of oil phase gel, 0.56 wt% of water phase gel, 0.364 wt% of pH regulator, 0.1 wt% of preservative and the balance of water.
Example 2
(1) Accurately weighing 1.5g of candelilla wax, adding into 48.5g of shea butter, and stirring at 75deg.C to dissolve to obtain oleogel;
(2) Accurately weighing 0.38g of locust bean gum, adding into 33.33g of 0.1mol/L hydrochloric acid solution, and stirring at 80 ℃ to dissolve to obtain hydrogel;
(3) Accurately weighing 0.20g of plant polyphenol (wherein mangiferin 0.08g, naringin 0.04g and naringenin 0.08 g) and dissolving in 16.67g of 0.1mol/L sodium hydroxide solution, wherein ultrasonic power is 85W, and the plant polyphenol composition is dissolved for 30s in an auxiliary way to obtain a plant polyphenol composition aqueous solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 2:1, stirring at 15000rpm for 2min by a high-speed shearing machine to obtain hydrogel containing plant polyphenol, adding the obtained hydrogel containing plant polyphenol and the oil gel prepared by the component (1) into a vacuum emulsifying machine according to a weight ratio of 1:1, adding hydrochloric acid to adjust the pH value to 5.0, and adding 0.04g of kathon; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 Mpa, temperature 52 deg.C, homogenizing speed 15000rpm, homogenizing time 3min, and making into double gel composition.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 20min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 4 ℃ to obtain the double gel paste containing plant polyphenol.
The composition of the dual gel product prepared in this example was: 0.2 wt% of plant polyphenol, 48.5 wt% of grease, 1.5 wt% of oil phase gel, 0.38 wt% of water phase gel, 0.345 wt% of pH regulator, 0.04 wt% of preservative and the balance of water.
Example 3
(1) Accurately weighing 1.05g of beeswax, adding into 44.4g of jojoba oil, and stirring at 67 ℃ to dissolve to obtain oleogel;
(2) Precisely weighing 0.95g of beta-glucan, adding the beta-glucan into 32.35g of 0.1mol/L hydrochloric acid solution, and stirring at 78 ℃ to dissolve the beta-glucan to obtain hydrogel;
(3) Accurately weighing 0.22g of plant polyphenol (wherein mangiferin 0.06g, naringin 0.07g and naringenin 0.09 g) and dissolving in 22.0g of 0.1mol/L sodium hydroxide solution, wherein ultrasonic power is 85W, and auxiliary dissolving is carried out for 25s to obtain a plant polyphenol composition aqueous solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 1.5:1, stirring at 18000rpm for 1min by a high-speed shearing machine to obtain a hydrogel containing plant polyphenol, adding the obtained hydrogel containing plant polyphenol and the oil gel prepared by the component (1) into a vacuum emulsifying machine according to a weight ratio of 1.25:1, adding hydrochloric acid to adjust the pH value to 4.0, and adding 0.01g of kathon; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 70 deg.C, homogenizing speed 18000rpm, homogenizing time 2min, and making into double gel composition.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 15min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 18 ℃ to obtain the double gel paste containing plant polyphenol.
The composition of the dual gel product prepared in this example was: 0.22 wt% of plant polyphenol, 44.4 wt% of grease, 1.05 wt% of oil phase gel, 0.95 wt% of water phase gel, 0.358 wt% of pH regulator, 0.01 wt% of preservative and the balance of water.
Example 4
(1) Accurately weighing 2.9g of candelilla wax, adding into 63.7g of glycerol, and stirring at 70deg.C to dissolve to obtain oleogel;
(2) Accurately weighing 0.15g of flaxseed gum, adding into 22.0g of 0.1mol/L hydrochloric acid solution, stirring at 85 ℃ to dissolve the flaxseed gum to obtain hydrogel;
(3) Accurately weighing 0.12g of plant polyphenol (wherein mangiferin 0.02g, naringin 0.04g and naringenin 0.06 g) and dissolving in 11.0g of 0.1mol/L sodium hydroxide solution, wherein ultrasonic power is 45W, and 60s assist in dissolving the plant polyphenol composition to obtain a plant polyphenol composition aqueous solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 2:1, stirring for 3min by a high-speed shearing machine at 10000rpm to obtain a hydrogel containing plant polyphenol, adding the obtained hydrogel containing plant polyphenol and the oil gel prepared by the component (1) into a vacuum emulsifying machine according to a weight ratio of 1:2, adding hydrochloric acid to adjust the pH value to 6.0, and adding 0.01g of kathon; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 82 deg.C, homogenizing speed 10000rpm, homogenizing time 5min, and making into double gel composition.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 30min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 25 ℃ to obtain the double gel paste containing plant polyphenol.
The composition of the dual gel product prepared in this example was: 0.12 wt% of plant polyphenol, 63.7 wt% of grease, 2.9 wt% of oil phase gel, 0.15 wt% of water phase gel, 0.227 wt% of pH regulator, 0.01 wt% of preservative and the balance of water.
Example 5
(1) Accurately weighing 0.75g of beeswax, adding into 32.5g of glycerol, and stirring at 70deg.C to dissolve to obtain oleogel;
(2) Precisely weighing 1.2g of beta-glucan, adding the beta-glucan into 32g of 0.1mol/L hydrochloric acid solution, and stirring at 85 ℃ to dissolve the beta-glucan to obtain hydrogel;
(3) Accurately weighing 0.25g of plant polyphenol (wherein mangiferin 0.125g, naringin 0.025g and naringenin 0.1 g) and dissolving in 33g of 0.1mol/L sodium hydroxide solution, wherein ultrasonic power is 45W, and 60s assist in dissolving the plant polyphenol composition to obtain a plant polyphenol composition aqueous solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 1:1, stirring for 3min by a high-speed shearing machine at 10000rpm to obtain a hydrogel containing plant polyphenol, adding the obtained hydrogel containing plant polyphenol and the oil gel prepared by the component (1) into a vacuum emulsifying machine according to a weight ratio of 2:1, adding hydrochloric acid to adjust the pH value to 6.0, and then adding 0.21g of phenoxyethanol; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 82 deg.C, homogenizing speed 10000rpm, homogenizing time 5min, and making into double gel composition.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 30min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 25 ℃ to obtain the double gel paste containing plant polyphenol.
The composition of the dual gel product prepared in this example was: 0.25 wt% of plant polyphenol, 32.5 wt% of grease, 0.75 wt% of oil phase gel, 1.2 wt% of water phase gel, 0.399 wt% of pH regulator, 0.21 wt% of preservative and the balance of water.
Example 6
(1) Accurately weighing 1.5g of candelilla wax, adding into 48.5g of shea butter, and stirring at 75deg.C to dissolve to obtain oleogel;
(2) Accurately weighing 0.38g of locust bean gum, adding into 33.33g of 0.1mol/L hydrochloric acid solution, and stirring at 80 ℃ to dissolve to obtain hydrogel;
(3) Accurately weighing 0.20g of plant polyphenol (wherein mangiferin 0.04g, naringin 0.08g and naringenin 0.08 g) and dissolving in 16.67g of 0.1mol/L sodium hydroxide solution, wherein ultrasonic power is 85W, and the plant polyphenol composition is dissolved for 30s in an auxiliary way to obtain a plant polyphenol composition aqueous solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 2:1, stirring at 15000rpm for 2min by a high-speed shearing machine to obtain hydrogel containing plant polyphenol, adding the obtained hydrogel containing plant polyphenol and the oil gel prepared by the component (1) into a vacuum emulsifying machine according to a weight ratio of 1:1, adding hydrochloric acid to adjust the pH value to 5.0, and adding 0.04g of kathon; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 52 deg.C, homogenizing speed 15000rpm, homogenizing time 3min, and making into double gel composition.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 20min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 4 ℃ to obtain the double gel paste containing plant polyphenol.
The composition of the dual gel product prepared in this example was: 0.2 wt% of plant polyphenol, 48.5 wt% of grease, 1.5 wt% of oil phase gel, 0.38 wt% of water phase gel, 0.345 wt% of pH regulator, 0.04 wt% of preservative and the balance of water.
Example 7
(1) Accurately weighing 1.5g of candelilla wax, adding into 48.5g of shea butter, and stirring at 75deg.C to dissolve to obtain oleogel;
(2) Accurately weighing 0.38g of locust bean gum, adding into 33.33g of 0.1mol/L hydrochloric acid solution, and stirring at 80 ℃ to dissolve to obtain hydrogel;
(3) Accurately weighing 0.20g mangiferin, dissolving in 16.67g 0.1mol/L sodium hydroxide solution, and performing ultrasonic power of 85W for 30s to assist in dissolving mangiferin to obtain mangiferin water solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 2:1, stirring at 15000rpm for 2min by a high-speed shearing machine to obtain a mangiferin-containing hydrogel, adding the obtained mangiferin-containing hydrogel and the oleogel prepared in the step (1) into a vacuum emulsifying machine according to a weight ratio of 1:1, adding hydrochloric acid to adjust the pH value to 5.0, and adding 0.04g of kathon; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 52 deg.C, homogenizing speed 15000rpm, homogenizing time 3min, and making into mangiferin double gel.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 20min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 4 ℃ to obtain the mangiferin-containing double gel paste.
The composition of the dual gel product prepared in this example was: 0.2 wt% of plant polyphenol, 48.5 wt% of grease, 1.5 wt% of oil phase gel, 0.38 wt% of water phase gel, 0.345 wt% of pH regulator, 0.04 wt% of preservative and the balance of water.
Example 8
(1) Accurately weighing 1.5g of candelilla wax, adding into 48.5g of shea butter, and stirring at 75deg.C to dissolve to obtain oleogel;
(2) Accurately weighing 0.38g of locust bean gum, adding into 33.33g of 0.1mol/L hydrochloric acid solution, and stirring at 80 ℃ to dissolve to obtain hydrogel;
(3) Accurately weighing 0.20g of naringin, dissolving in 16.67g of 0.1mol/L sodium hydroxide solution, and performing ultrasonic power of 85W for 30s to assist in dissolving naringin to obtain naringin aqueous solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 2:1, stirring at 15000rpm for 2min by a high-speed shearing machine to obtain hydrogel containing plant polyphenol, adding the obtained hydrogel containing naringin and the oil gel prepared by the component (1) into a vacuum emulsifying machine according to a weight ratio of 1:1, adding hydrochloric acid to adjust the pH value to 50, and then adding 0.04g of kathon; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 52 deg.C, homogenizing speed 15000rpm, homogenizing time 3min, and making into naringin double gel.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 20min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 4 ℃ to obtain the double gel paste containing naringin.
The composition of the dual gel product prepared in this example was: 0.2 wt% of plant polyphenol, 48.5 wt% of grease, 1.5 wt% of oil phase gel, 0.38 wt% of water phase gel, 0.345 wt% of pH regulator, 0.04 wt% of preservative and the balance of water.
Example 9
(1) Accurately weighing 1.5g of candelilla wax, adding into 48.5g of shea butter, and stirring at 75deg.C to dissolve to obtain oleogel;
(2) Accurately weighing 0.38g of locust bean gum, adding into 33.33g of 0.1mol/L hydrochloric acid solution, and stirring at 80 ℃ to dissolve to obtain hydrogel;
(3) Accurately weighing 0.20g naringenin, dissolving in 16.67g 0.1mol/L sodium hydroxide solution, and performing ultrasonic power of 85W for 30s to assist in dissolving naringenin to obtain naringenin Pi Sushui solution;
(4) Mixing the components (2) and (3) according to a weight ratio of 2:1, stirring at 15000rpm for 2min by a high-speed shearing machine to obtain naringenin-containing hydrogel, adding the naringenin-containing hydrogel and the oil gel prepared by the component (1) into a vacuum emulsifying machine according to a weight ratio of 1:1, adding hydrochloric acid to adjust the pH value to 5.0, and adding 0.04g of kathon; then, the vacuum emulsifying machine sets parameters: vacuum degree-0.097 MPa, temperature 52 deg.C, homogenizing speed 15000rpm, homogenizing time 3min, and making into naringenin double gel.
(5) The total preparation time from the end of the step (3) to the end of the step (4) is 20min;
(6) And (3) canning the double gel obtained in the step (5) into a packaging bottle, and cooling to room temperature at 4 ℃ to obtain the double gel paste containing naringenin.
The composition of the dual gel product prepared in this example was: 0.2 wt% of plant polyphenol, 48.5 wt% of grease, 1.5 wt% of oil phase gel, 0.38 wt% of water phase gel, 0.345 wt% of pH regulator, 0.04 wt% of preservative and the balance of water.
The external appearance patterns of the plant polyphenol-containing double gel pastes prepared in examples 1 to 9 are shown in fig. 1. As shown in FIG. 1, the plant polyphenol-containing double gel pastes prepared in examples 1 to 9 are all in a gel state, have certain spreadability, have no fluidity (no liquid flow after inversion), have uniform paste distribution, and have no obvious layering, milk precipitation and other phenomena.
The plant polyphenol retention rate of the plant polyphenol-containing double gel paste prepared in example 3 during storage was measured by the following test method: the sample (plant polyphenol solution or plant polyphenol double gel paste) was centrifuged at 3000×g for 15min, and then 1mL of 30% DMSO solution containing 0.1% acetic acid in the supernatant was diluted, and the absorbance at 278nm was measured using an ultraviolet-visible spectrophotometer, to calculate the content of plant polyphenol. Standard curve: y= 0.0331X-0.0037, r 2 = 0.9996, linear range 2.5-80 μg/mL. The retention (R) of plant polyphenols is calculated as follows:
R(％)＝(C t /C i )×100
wherein C is i C for the concentration of plant polyphenols in the initial sample t To preserve the concentration of plant polyphenols in samples at different time phases.
As shown in fig. 2, it can be seen that the dual gel paste containing plant polyphenol prepared by the present disclosure can effectively slow down crystallization, precipitation and degradation of the plant polyphenol composition. Compared with the plant polyphenol composition solution, the retention rate of plant polyphenol in the double gel paste is obviously improved.
The plant polyphenol-containing double gel pastes prepared in examples 1 to 5 were tested for centrifugal stability, and the test method was as follows: the sample was placed in a 15mL centrifuge tube and centrifuged at 4000 Xg for 15min at 25℃to observe the phase separation of the dual gel paste, the results of which are shown in FIG. 3.
The appearance of the plant polyphenol-containing double gel pastes prepared in examples 1 to 5 is shown in fig. 1, but the centrifugal stability (as shown in fig. 3) of the plant polyphenol-containing double gel pastes prepared in examples 4 to 5 is inferior to that of the plant polyphenol-containing double gel pastes prepared in examples 1 to 3, and after the plant polyphenol-containing double gel pastes prepared in examples 4 to 5 are centrifuged, the upper layer of the test tube shows a remarkable oil precipitation phenomenon.
The plant polyphenol-containing double gel pastes prepared in examples 2 and 6 to 9 were tested for anti-dermatitis effect.
The testing method comprises the following steps: acute ear stimulation experiment in mice: BALB/C female mice of SPF grade of 6-8 weeks are selected and fed for one week before molding. After one week, the skin behind the ear was stimulated with 20 μl of an acetone solution containing 15% TPA (phorbol 12-tetradecanoate 13-acetate) on the outside of the mouse ear to induce skin inflammation and ulceration. The ears of the blank group are only coated with acetone, the ears of the model group are stimulated with TPA, the ears of the administration group are stimulated with TPA for 3 hours, and then the double gel paste containing plant polyphenol prepared in the coating example is coated with the paste for 5 days continuously, and ear tissues are taken for observation. The test results are shown in fig. 4.
It can be seen that after TPA stimulation, the mouse ear showed significant reddening and swelling and skin loss, indicating successful modeling of the mouse ear dermatitis. In addition, after the treatment of the mangiferin double gel paste (example 7), naringin double gel paste (example 8), naringin Pi Sushuang gel paste (example 9) and plant polyphenol composition double gel paste (examples 2 and 6) having the same polyphenol content were given to the ears of the mice, the plant polyphenol composition double gel paste of example 2 had the best effect of reducing the red swelling and skin damage symptoms of the ears of the mice, and the skin surface was smooth without the red swelling and skin damage symptoms; the polyphenol composition double gel cream of example 6 also had a reducing effect on the red swelling and skin damage symptoms of the mouse ear, but the overall effect was slightly worse than that of example 2; examples 7-9 also repaired a large portion of the tissue at the inflammatory focus with only a small number of skin lesions.
The preferred embodiments of the present disclosure have been described in detail above, but the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solutions of the present disclosure within the scope of the technical concept of the present disclosure, and all the simple modifications belong to the protection scope of the present disclosure.
In addition, the specific features described in the foregoing embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, the present disclosure does not further describe various possible combinations.
Moreover, any combination between the various embodiments of the present disclosure is possible as long as it does not depart from the spirit of the present disclosure, which should also be construed as the disclosure of the present disclosure.
Claims (3)
1. The double gel composition is characterized by comprising, by weight, 0.18-0.22% of plant polyphenol, 38-48.5% of grease, 1.05-2.0% of oil phase gel, 0.38-0.95% of aqueous phase gel, 0.35-0.47% of auxiliary agent and the balance of water, wherein the plant polyphenol is mangiferin, naringin and naringenin, and the weight ratio of mangiferin, naringin and naringenin is 1: (0.5-1.2), wherein (1-1.5) the grease is one or more selected from jojoba oil, shea butter and glycerin, the oil phase gel is candelilla wax and/or beeswax, and the water phase gel is one or more selected from locust bean gum, flaxseed gum and beta-glucan.
2. The dual gel composition of claim 1, wherein the auxiliary comprises a preservative and a pH adjuster, the preservative being present in an amount of 0.01-0.21% by weight and the pH adjuster being present in an amount of 0.23-0.40% by weight.
3. The dual gel composition of claim 2, wherein the preservative is pinacol and/or phenoxyethanol; the pH regulator is sodium hydroxide and/or hydrochloric acid.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999062497A1 (en) * | 1998-06-03 | 1999-12-09 | Aiache Jean Marc | Stable gel mixture in the form of a mixture of oleogel and aqueous gel |
| US6187323B1 (en) * | 1998-06-03 | 2001-02-13 | Zentrx, Inc. | Stable gel mixture in the form of a mixture of oleogel and aqueous gel, process for its preparation, pharmaceutical and cosmetic compositions comprising it, and use of the pharmaceutical compositions |
| CN106937917A (en) * | 2016-01-05 | 2017-07-11 | 河南工业大学 | A kind of preparation of double gel cosmetic products based on organogel and hydrogel |
| KR20190053763A (en) * | 2017-11-10 | 2019-05-20 | (주)아모레퍼시픽 | Cosmetic composition of bigel type |
| CN111700264A (en) * | 2020-06-30 | 2020-09-25 | 江南大学 | Preparation method and application of bicontinuous emulsion gel |
| CN114191381A (en) * | 2021-11-29 | 2022-03-18 | 华南农业大学 | Temperature-sensitive type double gel with controllable simulated plastic fat, controllable oral texture and controllable digestion behavior, and preparation and application thereof |
| CN114304283A (en) * | 2021-12-15 | 2022-04-12 | 华南理工大学 | Preparation method of food-grade double gel based on grease gel and hydrogel |
| CN114847396A (en) * | 2022-04-24 | 2022-08-05 | 华南农业大学 | Double-gel capable of printing protein network and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2445539A (en) * | 2006-12-29 | 2008-07-16 | Ardana Bioscience Ltd | Bigel composition |
-
2022
- 2022-10-24 CN CN202211302982.0A patent/CN115429703B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999062497A1 (en) * | 1998-06-03 | 1999-12-09 | Aiache Jean Marc | Stable gel mixture in the form of a mixture of oleogel and aqueous gel |
| US6187323B1 (en) * | 1998-06-03 | 2001-02-13 | Zentrx, Inc. | Stable gel mixture in the form of a mixture of oleogel and aqueous gel, process for its preparation, pharmaceutical and cosmetic compositions comprising it, and use of the pharmaceutical compositions |
| CN106937917A (en) * | 2016-01-05 | 2017-07-11 | 河南工业大学 | A kind of preparation of double gel cosmetic products based on organogel and hydrogel |
| KR20190053763A (en) * | 2017-11-10 | 2019-05-20 | (주)아모레퍼시픽 | Cosmetic composition of bigel type |
| CN111700264A (en) * | 2020-06-30 | 2020-09-25 | 江南大学 | Preparation method and application of bicontinuous emulsion gel |
| CN114191381A (en) * | 2021-11-29 | 2022-03-18 | 华南农业大学 | Temperature-sensitive type double gel with controllable simulated plastic fat, controllable oral texture and controllable digestion behavior, and preparation and application thereof |
| CN114304283A (en) * | 2021-12-15 | 2022-04-12 | 华南理工大学 | Preparation method of food-grade double gel based on grease gel and hydrogel |
| CN114847396A (en) * | 2022-04-24 | 2022-08-05 | 华南农业大学 | Double-gel capable of printing protein network and preparation method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| "Bigels as drug delivery systems: From their components to their applications";Martin-Illana, A 等;《》;第27卷(第4期);第1008-1026页 * |
| "Encapsulation of hydrophobic and low-soluble polyphenols into nanoliposomes by pH-driven method: naringenin and naringin as model compounds";Mianhong Chen 等;《foods》;第10卷;第963-977页 * |
| "Key characteristics and modelling of bigels systems: A review";Shakeel, Ahmad 等;《Materials Science & Engineering C》;第97卷;第932-953页 * |
| "不同品种芒果核中多酚类化合物的鉴定及抗氧化活性分析";邢宇航 等;《食品工业科技》;第43卷(第09期);第63-70页 * |
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