CN115414404A - Extraction method of Clitoria ternatea flower - Google Patents
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Abstract
The invention belongs to the technical field of biomass extraction, and particularly relates to a method for extracting butterflybush flower. The invention provides a method for extracting Clitoria ternatea flower, which comprises the following steps: removing stamen, calyx and pedicel of Clitoria ternatea flower to obtain Clitoria ternatea flower petal; drying and crushing the sphenoidea petals to obtain sphenoidea flower powder; sequentially carrying out ultrasonic extraction and solid-liquid separation on the butterflybush flower powder and an ethanol solution to obtain an extracting solution; the volume fraction of ethanol in the ethanol solution is 35-80%; removing the solvent from the extracting solution to obtain a sphenoidea flower extract, wherein the sphenoidea flower extract contains anthocyanin. The anthocyanin extraction amount in the sphenoidea extract obtained by the invention reaches 13.604 +/-0.145 mg/g; experiments prove that the concentration of the sphenoidea flower extract is 0.8mg/mL, the extract can be used for treating DPPH free radicals and ABTS + The clearance rate of free radicals is 87.83 percent and99％。
Description
Technical Field
The invention belongs to the technical field of biomass extraction, and particularly relates to a method for extracting butterflybush flower.
Background
Anthocyanins (anthocyanins) are a class of water-soluble natural pigments that are ubiquitous in dark plants. Along with the more and more deep understanding of the harmfulness of synthetic pigments, natural pigments are more and more valued under the current background of focusing on health care and food safety. The development and development of natural pigments to replace artificial pigments as colorants has become a development trend in the food, pharmaceutical and cosmetic industries.
The Clitoria ternata (Clitoria ternata) is a typical perennial tropical vine plant of the genus Clitoria of the family Leguminosae, contains abundant anthocyanin, has higher content than that of general plants, has a long flowering phase (4-11 months), is high in yield, is easy to cultivate, has more obvious advantages in tropical and subtropical regions, and is an ideal natural raw material for anthocyanin. In addition, the anthocyanidin in the blue spherule flowers is mainly acylated delphinidin (ternatins), and the anthocyanidin has better heat resistance and light resistance and more stable chemical property than the common anthocyanidin without acylation groups.
At present, the extraction of anthocyanin in sphenoides has the problems of complex extraction process and low extraction efficiency: for example, chinese patent CN110437197a discloses a method for extracting anthocyanidin from sphena, which comprises the steps of degreasing, leaching, ultrafiltration, concentration, alcohol precipitation, resin adsorption, gradient desorption, concentration and drying, etc., to obtain an anthocyanidin product; chinese patent CN109400569A discloses a method for extracting and purifying anthocyanidin from sphenoides, which comprises the steps of enzymolysis, concentration, adsorption, gradient elution, concentration, drying and the like. Therefore, it is highly desirable to develop a method for extracting Clitoria ternatea flower which is easy to operate and has high extraction efficiency.
Disclosure of Invention
The extraction method of the sphenoidea flower is simple to operate and high in extraction efficiency, and the obtained extract of the sphenoidea flower is high in anthocyanin content.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for extracting sphenoidea, which comprises the following steps:
removing stamen, calyx and pedicel of Clitoria ternatea flower to obtain Clitoria ternatea flower petal;
drying and crushing the sphenoides petals to obtain sphenoides flower powder;
sequentially carrying out ultrasonic extraction and solid-liquid separation on the butterflybush flower powder and an ethanol solution to obtain an extracting solution; the volume fraction of ethanol in the ethanol solution is 35-80%;
removing the solvent from the extracting solution to obtain a sphenoidea flower extract, wherein the sphenoidea flower extract contains anthocyanin.
Preferably, the ultrasonic extraction time is 20-50 min.
Preferably, the volume fraction of ethanol in the ethanol solution is 50 to 65 percent.
Preferably, the material-liquid ratio of the ultrasonic extraction is 1g.
Preferably, the temperature of the ultrasonic extraction is 40-70 ℃.
Preferably, the power of the ultrasonic extraction is 240-600W.
Preferably, the solvent is removed and then drying is carried out.
Preferably, the method for removing the solvent comprises distillation under reduced pressure.
Preferably, the particle size of the sphenoidea flower powder is 0.180-0.425 mm.
The invention provides a method for extracting Clitoria ternatea flower, which comprises the following steps: removing stamen, calyx and pedicel of Clitoria ternatea flower to obtain Clitoria ternatea flower petal; drying and crushing the sphenoidea petals to obtain sphenoidea flower powder; sequentially carrying out ultrasonic extraction and solid-liquid separation on the butterflybush flower powder and an ethanol solution to obtain an extracting solution; the volume fraction of ethanol in the ethanol solution is 35-80%; removing the solvent from the extracting solution to obtain a sphenoidea flower extract, wherein the sphenoidea flower extract contains anthocyanin.
Compared with the prior art, the invention has the following beneficial effects:
the method for extracting the sphenoidea flower has the advantages that the petals of the sphenoidea flower are crushed and then subjected to ultrasonic extraction by using the ethanol solution, so that the contact area between a sample and the ethanol solution serving as the extraction solvent is increased, meanwhile, the petals of the sphenoidea flower are limited, stamens, calyxes and pedicels of the flower are removed, the interference of impurities such as fat, protein and the like is reduced, more anthocyanin is favorably extracted, and the ethanol solution is more suitable for extracting the anthocyanin.
Compared with an aqueous extract, the content of anthocyanin in the Clitoria ternatea flower extract is obviously improved and different in composition, the content of anthocyanin is measured by adopting a single PH method, when the PH value is 1.0, the maximum absorption wavelength is 540nm, and the maximum absorption wavelength of the aqueous extract is 620nm.
Furthermore, the invention only uses ethanol solution for extraction, and the used reagent has low toxicity and is environment-friendly. The invention also limits the optimal ultrasonic extraction time, material-liquid ratio, ethanol volume fraction and ultrasonic extraction temperature for extracting the sphenoidea flower, and further improves the extraction efficiency, and the example data shows that the anthocyanin in the sphenoidea flower extract obtained by the invention can reach 13.693mg/g.
The Clitoria ternatea flower extract of the present invention has been shown to have better DPPH radical scavenging activity and ABTS scavenging activity as a crude extract mixture + Free radical activity and strong reducing power.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
In the figure 1, A is the dry powder of sphenoidea flower, and B is the extracted and purified sphenoidea anthocyanin;
in fig. 2, a is a sample directly dried without purification after the extract of the sphenoidea, B is a sample dried with impurities lost in the purification process, and C is extracted and purified sphenoidea anthocyanin;
in FIG. 3, 1 is the extraction liquid directly drying the sample and adding water to dissolve, 2 is the eluent dried product and adding water to dissolve, and 3 is the extracted and purified anthocyanin sample and adding water to dissolve;
FIG. 4 is an absorption spectrum of anthocyanidin in Clitoria ternifolia at different pH;
FIG. 5 shows the effect of Clitoria ternatea extract on DPPH radical scavenging;
FIG. 6 shows the ABTS pair of Clitoria ternatea extract + The result of the scavenging action of free radicals;
FIG. 7 is the total reducing power results of Clitoria ternatea extract.
Detailed Description
The invention provides a method for extracting Clitoria ternatea flower, which comprises the following steps:
removing stamen, calyx and pedicel of Clitoria ternatea flower to obtain Clitoria ternatea flower petal;
drying and crushing the sphenoidea petals to obtain sphenoidea flower powder;
sequentially carrying out ultrasonic extraction and solid-liquid separation on the butterflybush flower powder and an ethanol solution to obtain an extracting solution; the volume fraction of ethanol in the ethanol solution is 35-80%;
removing the solvent from the extracting solution to obtain a sphenoidea flower extract, wherein the sphenoidea flower extract contains anthocyanin.
In the present invention, reagents and instruments used are commercially available in the art unless otherwise specified.
The method removes stamen, calyx and pedicel of Clitoria ternatea flower to obtain Clitoria ternatea flower petal.
In the invention, the sphenoidea petals are preferably obtained by removing pistils, calyces and pedicles from the sphenoidea flowers, so that the interference of impurities such as fat, protein and the like is reduced after the pistils, the calyces and the pedicles are removed, and the subsequent ultrasonic extraction is facilitated.
After the sphenoides petals are obtained, the sphenoides petals are dried and crushed to obtain the sphenoides flower powder.
In the present invention, the drying temperature is preferably 40 to 60 ℃, more preferably 50 ℃, and the drying time is preferably 13 to 20 hours, more preferably 16 hours. The invention has no special requirements on the crushing mode, and the method can be realized by adopting a mode commonly used by a person skilled in the art.
In the present invention, the particle size of the butterflybush flower powder is preferably 0.180 to 0.425mm, and in a specific embodiment of the present invention, the butterflybush flower powder is sieved by a 40-mesh sieve.
After the sphenoidea powder is obtained, the method carries out ultrasonic extraction and solid-liquid separation on the sphenoidea powder and an ethanol solution in sequence to obtain an extracting solution.
In the present invention, the time for the ultrasonic extraction is preferably 20 to 50min, and more preferably 20 to 30min.
In the invention, the feed-liquid ratio of the ultrasonic extraction is preferably 1g.
In the present invention, the volume fraction of ethanol in the ethanol solution is preferably 50 to 65%, more preferably 65%.
In the present invention, the temperature of the ultrasonic extraction is preferably 40 to 70 ℃, more preferably 50 ℃.
In the present invention, the power of the ultrasonic extraction is preferably 240 to 600W, more preferably 480W.
The solid-liquid separation mode is not specially required, if centrifugation is adopted, the rotation speed of the centrifugation is preferably 6000r/min, the time is preferably 7min, and the supernatant after the centrifugation is the extracting solution.
After the extracting solution is obtained, the solvent of the extracting solution is removed to obtain the sphenoidea extract.
In the present invention, the method for removing the solvent preferably comprises reduced pressure distillation, and in the specific embodiment of the present invention, such as rotary evaporation, the present invention has no special requirement on the specific conditions of the rotary evaporation, and the conditions commonly used by those skilled in the art can be adopted.
In the present invention, the solvent removal preferably further comprises drying, the drying is preferably vacuum drying, the temperature of the vacuum drying is preferably 50 ℃, and the vacuum degree is preferably 0.1MPa. The invention has no special requirement on the vacuum drying time, and the buttercup flower extract is dried to constant weight.
In order to further illustrate the present invention, the following detailed description of the extraction method of Clitoria ternatea provided by the present invention is provided with reference to the accompanying drawings and examples, which should not be construed as limiting the scope of the present invention.
Example 1
1. Ultrasonic extraction single factor experiment
Removing stamen, calyx and pedicel of the sphenoides fresh flower, drying at 50 deg.C for 16 hr, pulverizing, sieving with 40 mesh sieve to obtain powder, sealing, and storing in dark place.
Weighing sphenoidea flower powder, adding an ethanol solution, shaking uniformly, putting into an ultrasonic instrument for extraction, centrifuging, taking supernate to obtain a sphenoidea flower extract, and determining the anthocyanin content in the extract, wherein the specific extraction conditions are as follows:
1) Extracting with ethanol solutions of different ethanol volume fractions (0%, 20%, 35%, 50%, 65%, 80%, 85%, 90%, 95%) as solvents at a feed-liquid ratio of 1;
2) An ethanol solution with an ethanol volume fraction of 50% is used as a solvent, the material-liquid ratio is (1;
3) The feed-liquid ratio is 1;
4) The feed-liquid ratio is 1;
5) The material-liquid ratio is 1.
Taking the anthocyanin content in the extracting solution as an investigation index, slightly increasing the extraction amount of the anthocyanin along with the increase of the ultrasonic power, wherein the significant difference (P < 0.05) exists between the ultrasonic power of 0W and 240W-600W, slightly decreasing the extraction amount along with the increase of the ultrasonic power after 480W reaches the maximum value of 13.501 +/-0.306 mg/g, and performing single-factor variance analysis on the power to obtain that the difference between the powers is not significant, which indicates that the ultrasonic power is a non-main factor, so 480W is selected as a fixed ultrasonic power in subsequent experiments, and the factors of an orthogonal experiment are determined to comprise a material-liquid ratio, an ethanol volume fraction, an ultrasonic temperature and ultrasonic time.
2. According to the result of single factor, the material-liquid ratio (A), the volume fraction (B) of the ethanol, the ultrasonic temperature (C) and the ultrasonic time are selectedThe method (D) is a factor influencing the extraction amount of the anthocyanidin of sphenoides, each factor is set to 4 levels, and L16 (4) is designed 5 ) Orthogonal experiment, optimizing and designing the extraction process, discussing the optimal extraction conditions, wherein the specific factors and the horizontal settings are shown in table 1, and the experimental results are shown in table 2.
TABLE 1 orthogonal experiment factor horizon
TABLE 2 results of orthogonal experiments
TABLE 3 analysis of variance results
Determination of optimal extraction conditions:
the results of orthogonal experiments are shown in Table 2, and the optimal process condition for extracting the sphenoides anthocyanin by the aid of ultrasonic is A 4 B 3 C 2 D 1 Namely, the material-liquid ratio is 1. From the magnitude of the range R, the biggest influence on the extraction amount of the sphenopigma anthocyanin is D (ultrasonic time), and then A (feed-liquid ratio), B (ethanol volume fraction) and C (ultrasonic temperature) in sequence, wherein the R values of the ethanol concentration and the ultrasonic temperature are smaller than that of the empty column E, which shows that the two factors are relatively non-main factors in the horizontal range selected by the orthogonal experimental design. The 4-factor orthogonal data are taken for analysis of variance, the results are shown in Table 3, and the material-liquid ratio, the ethanol volume fraction, the ultrasonic time and the ultrasonic temperature level selected in the orthogonal test are alignedThere was no significant difference in the effect of the amount of extraction of the melanin (p)>0.05). The highest extraction amount in the orthogonal experiment design is experiment No. 12, namely the material-liquid ratio is 1, the volume fraction of ethanol is 80%, the ultrasonic time is 20min, the ultrasonic temperature is 50 ℃, and the optimization difference from the orthogonal experiment is that the material-liquid ratio and the volume fraction of ethanol are different.
Verifying according to ultrasonic extraction conditions optimized by orthogonal experiments, concentrating the obtained extracting solution to 25-30 mL, and then determining, wherein the anthocyanin extraction amount obtained by 3 times of repeated experiments is 13.682, 13.693 and 13.437mg/g respectively, mean plus or minus SD is 13.604 plus or minus 0.145mg/g, which is superior to other combinations, and the analysis result is reliable.
The method comprises the steps of purifying the sphenoidea flower extract obtained under ultrasonic extraction conditions optimized according to orthogonal experiments, wherein the purification method refers to Zhang Panpan, gu Chengtao and Wang Li research on blueberry pomace anthocyanin purified by macroporous resin [ J ]. Anhui academy of science and technology, 2017,31 (2): 7.", A in figure 1 is dry powder of sphenoidea flower, and B in figure 1 is extracted and purified sphenoidea anthocyanin. A in figure 2 is a sample which is directly dried without purification after the extraction of the sphenoidea flower, and is sticky and not powdered because of many impurities, B in figure 2 is a sample after the impurities lost in the purification process are dried, and C in figure 2 is the extracted and purified sphenoidea anthocyanin. In figure 3, 1 is that the extract is directly dried, the sample is dissolved by adding water, 2 is that the dried product of the eluent is dissolved by adding water, and 3 is that the purified anthocyanin-like product is dissolved by adding water; FIGS. 31, 2 and 3 correspond to the A, B, C sample of FIG. 2 after being dissolved in water.
Formula for calculating anthocyanin extraction amount
Extracting the sphenoidea flower powder, taking a sphenoidea flower extracting solution, adding a buffer solution with the pH value of 1.0 to dilute the extracting solution by a certain multiple, balancing for 40min at a constant temperature of 40 ℃, measuring an absorbance value at the position of the maximum absorption wavelength, wherein the calculation formula is shown as formula I:
in the formula: Δ A = A λmax -A 700nm (ii) a MW (component of cyanidin-3-glucoside)Quantum) =449.2g/mol; DF = dilution factor; v = final volume, mL; epsilon (molar extinction coefficient of cyanidin-3-glucoside) = 26900L/(mol cm); l = optical path length, 1cm; wt = product mass (butterflybush flower powder), mg.
Test example 1
Study on absorption characteristics of sphenoides anthocyanins
Taking the extract of the sphenoidea flower under the optimal process conditions (namely the feed-liquid ratio is 1:200, the volume fraction of ethanol is 65%, the ultrasonic time is 20min, and the ultrasonic temperature is 50 ℃), respectively adding buffer solutions with the pH value of 1.0 and the pH value of 4.5, diluting the extract by 20 times, balancing the extract at a constant temperature of 40 ℃ for 40min, carrying out wavelength scanning within the range of 200-760 nm, and showing the absorption spectra of the sphenoidea flower anthocyanin under different pH values in a graph shown in figure 4.
As can be seen in FIG. 4, the extract diluted with a buffer at pH 1.0 showed a maximum absorption at 540-550 nm, similar to the absorption curves of the plant extracts mentioned in the other studies; when the extract of the sphenoidea is diluted by a buffer solution with the pH value of 4.5, the extract of the sphenoidea is blue, is different from the light powder or light yellow of other plant extracts, two absorption peaks appear at 570 and 620nm respectively, so that the existence mode of the anthocyanin in the sphenoidea is different from that of other plants, the monomer anthocyanin content is low and is mostly polymerized anthocyanin, the polymerization brings absorption characteristics different from those of other anthocyanin to the sphenoidea, and the method is also consistent with the method that the polymerized anthocyanin can still keep color at the pH value of 4.5, and the special existence mode indicates that the determination of the content of the sphenoidea is not suitable for adopting a pH differential method for determining the total monomer anthocyanin content, because the principle of determining the content of the monomer anthocyanin by using the pH differential method is contrary to the assumption that the monomer anthocyanin content is based on the low or even no absorbance of the monomer or pure anthocyanin at the pH value of 4.5. The single pH method is used for measuring the total anthocyanin content containing polymeric anthocyanin and anthocyanin degradation products, the content of the pteria sphenanthera anthocyanin can be reasonably quantified, the absorbance of the extract at the pH value of 1.0 has certain correlation with the total anthocyanin content, the extraction effect is reflected to a certain extent, and the method can be applied to screening of factor levels in the extraction optimization process of the pteria sphenanthera anthocyanin.
Application example 1 measurement of antioxidant Activity
Extracting the clitoria sphenanthera flower powder according to the optimal extraction process of the embodiment 1, centrifuging the extracting solution (6000 r/min) for 7 minutes, taking supernatant, recovering ethanol, placing the supernatant in vacuum drying (50 ℃, and maintaining the vacuum degree at 0.1 MPa) to constant weight to obtain a clitoria sphenanthera flower extract, diluting the clitoria sphenanthera flower extract with deionized water, and preparing clitoria sphenanthera flower extract solutions (0.1, 0.2, 0.4, 0.6 and 0.8 mg/mL) with series concentrations.
1. DPPH free radical scavenging activity of Clitoria ternatea flower extract
Mixing flos Clerodendri Trichophylli extract solutions with series concentrations with 0.1mmol/L DPPH ethanol solution at a volume ratio of 1:1, standing at room temperature in dark for 30min, measuring absorbance at wavelength of 517nm, and recording as A 1 (ii) a After the deionized water and the DPPH ethanol solution react in the same way, the absorbance is measured and is recorded as A 0 (ii) a The absolute ethyl alcohol and the sample solution react in the same method, and the absorbance is measured and recorded as A 2 . The clearance rate is calculated by using ascorbic acid as a positive control and measuring the clearance rate by the same method, and the formula is shown as a formula II:
the result of the DPPH free radical scavenging activity of the butterflybush flower extract is shown in FIG. 5, and the DPPH free radical scavenging rate of the extract is increased along with the increase of the concentration in the range of 0.1-0.8 mg/mL. At 0.1-0.4 mg/mL, the clearance is rapidly increased from 28.22% to 85.85%, and when the concentration exceeds 0.4mg/mL, the clearance is maintained above 85%, and reaches the maximum value of the range at 0.8mg/mL, which is 87.83%. Under the same experimental method, the ascorbic acid is in the range of 0.002-0.01 mg/mL, and the clearance rate is 24.32-91.75%. The DPPH free radical clearance rate IC of the butterflybush flower extract and the ascorbic acid is obtained by Graphpad prism software calculation 50 The values were 0.168mg/mL and 0.004mg/mL, respectively.
2. ABTS eliminating extract of Clitoria ternatea + Free radical activity
Adding 7mmol/L ABTS + Mixing with 2.45mmol/L potassium persulfate solution in equal volume, and placing in dark place for 12 timesDiluting with distilled water to absorbance of 0.7 + -0.02 before use for 16h to obtain ABTS + And (4) working fluid. Mixing the extract solutions with ABTS + The working solution is uniformly mixed according to the volume proportion of 1:3, and after 6min of light-shielding reaction, the absorbance is measured at the wavelength of 734nm and is marked as A 1 (ii) a The deionized water is used to replace the sample, and the absorbance is measured by the same method and is marked as A 0 (ii) a Replacing ABTS with deionized water + The absorbance of each sample control group was measured by the same method and recorded as A 2 . The clearance rate calculation formula is the same as DPPH with ascorbic acid as a positive control.
ABTS eliminating extract of Clitoria ternatea + The results are shown in FIG. 6, which shows the ABTS pair of Clitoria ternatea extract + The clearance rate of free radicals is in the range of 0.1-0.8 mg/mL, which shows better positive correlation, the clearance rate is increased from 32.48% to 99%, and can reach 100% in 1mg/mL, and the ABTS of the Clitoria ternatea flower extract is calculated + The IC50 value of the free radical clearance rate is 0.151mg/mL, which indicates that the Clitoria ternatea flower extract has good antioxidant activity due to the fact that the Clitoria ternatea flower extract is rich in flavonoid anthocyanin. ABTS of positive control ascorbic acid at 0.006mg/mL + The free radical clearance rate is 44.69 percent and reaches 99.71 percent at 0.014mg/mL, and the IC is 50 The value was 0.007mg/mL. According to IC 50 It can be seen that the Clitoria ternatea flower extract of the present invention, as a crude extract mixture, has exhibited better ABTS clearance + Free radical activity.
3. Total reducing power of Clitoria ternatea flower extract
Mixing 500 μ L phosphate buffer solution (0.2 mol/L) with pH =6.6, 200 μ L herba Clerodendranthi Spicati extract solution (0.6, 0.8, 1.0, 1.2, 1.4 mg/mL) with 500 μ L potassium ferricyanide solution (1% by mass concentration), reacting at 50 deg.C for 20min, adding 500 μ L trichloroacetic acid (10% by mass concentration), mixing, reacting for 10min, collecting 500 μ L reaction solution, adding 500 μ L H 2 O, 100. Mu.L of a 0.1% iron trichloride solution, left to stand for 10min, and the absorbance was measured at a wavelength of 700 nm. Each absorbance was measured with ascorbic acid as a positive control and used to indicate the intensity of the reducing ability.
Antioxidant the Fe of potassium ferricyanide 3+ Reduction to Fe 2+ Reduced Fe 2+ The quantity is in a linear relationship with the absorbance at 700nm, and is used for representing the reduction force. The results of measuring the total reducing power of the butterflybush flower extract and ascorbic acid are shown in fig. 7. The absorbance of the sphenoidea flower extract is increased from 0.240 to 0.502 within the range of 0.6 to 1.4mg/mL, which shows that the reduction force is increased more smoothly, while the absorbance of the ascorbic acid corresponding to 0.02 to 0.1mg/mL is 0.188 to 0.8, which shows that the reduction force is increased more rapidly.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments of the present invention, and the embodiments are within the scope of the present invention.
Claims (9)
1. The extraction method of the sphenoidea is characterized by comprising the following steps:
removing stamen, calyx and pedicel of Clitoria ternatea flower to obtain Clitoria ternatea flower petal;
drying and crushing the sphenoidea petals to obtain sphenoidea flower powder;
sequentially carrying out ultrasonic extraction and solid-liquid separation on the butterflybush flower powder and an ethanol solution to obtain an extracting solution; the volume fraction of ethanol in the ethanol solution is 35-80%;
removing the solvent from the extracting solution to obtain a sphenoidea flower extract, wherein the sphenoidea flower extract contains anthocyanin.
2. The extraction method according to claim 1, wherein the time of the ultrasonic extraction is 20 to 50min.
3. The extraction process according to claim 1, wherein the volume fraction of ethanol in the ethanol solution is 50 to 65%.
4. The extraction method according to claim 1 or 3, wherein the material-liquid ratio of the ultrasonic extraction is 1g.
5. The extraction method according to claim 1, wherein the temperature of the ultrasonic extraction is 40 to 70 ℃.
6. The extraction method according to claim 1 or 5, wherein the power of the ultrasonic extraction is 240 to 600W.
7. The extraction process of claim 1, further comprising drying after the removal of the solvent.
8. The extraction process according to claim 1 or 7, wherein the solvent removal process comprises distillation under reduced pressure.
9. The extraction method according to claim 1, wherein the sphenoidea powder has a particle size of 0.180 to 0.425mm.
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| CN116462987A (en) * | 2023-04-27 | 2023-07-21 | 陕西全奥之星生物科技有限公司 | Preparation method of acid-stable butterfly bean flower blue pigment |
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| US11871754B1 (en) | 2023-07-26 | 2024-01-16 | King Faisal University | Promoting growth, yield, and salinity tolerance of Achillea fragrantissima |
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