CN115404164A - Sperm thawing treatment device - Google Patents

Sperm thawing treatment device Download PDF

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Publication number
CN115404164A
CN115404164A CN202211083188.1A CN202211083188A CN115404164A CN 115404164 A CN115404164 A CN 115404164A CN 202211083188 A CN202211083188 A CN 202211083188A CN 115404164 A CN115404164 A CN 115404164A
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constant temperature
cell culture
culture dish
sperm
screw rod
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CN202211083188.1A
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李吉炎
周毅
汪虹
刘秉鑫
余清
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Jingdezhen Maternal And Child Health Hospital Jingdezhen Maternal And Child Health And Family Planning Service Center
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Jingdezhen Maternal And Child Health Hospital Jingdezhen Maternal And Child Health And Family Planning Service Center
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Priority to CN202211083188.1A priority Critical patent/CN115404164A/en
Publication of CN115404164A publication Critical patent/CN115404164A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14Incubators; Climatic chambers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a sperm thawing processing device, which relates to the field of biotechnology and comprises a thermostat, wherein two groups of operating sleeves are arranged on the upper surface of a constant temperature control table, a moving mechanism, a linkage mechanism and a clamping mechanism are arranged, after a cell culture dish rises to the inner side of a clamping plate, a clamping cylinder is stretched and adjusted to enable the clamping plate to be abutted against the cell culture dish, the cell culture dish is clamped and fixed, an elevating cylinder is contracted and adjusted to enable the cell culture dish to return to an initial position, an operator performs subsequent processing on sperm in the thawed cell culture dish through the operating sleeves, after the processing, a second stepping motor is started to enable a second screw rod to rotate, the clamping plate moves the cell culture dish to the left side, the second stepping motor is closed after the cell culture dish moves to the lower end of an inverted microscope, and the operations such as sperm number evaluation, sperm vitality evaluation and the like are performed through the inverted microscope.

Description

Sperm thawing treatment device
Technical Field
The invention relates to the technical field of biology, in particular to a sperm thawing processing device.
Background
The semen cryopreservation and resuscitation technical research is the most effective assisted reproduction technology at present, semen resuscitation can realize artificial insemination at different time and different places, sperm motility is reduced, integrity of acrosome and plasma membrane is damaged and the like after sperm is thawed by the conventional sperm thawing equipment, so that the fertility rate and the survival rate are reduced, the conventional sperm thawing equipment and subsequent processing equipment are carried out on different equipment, operation is extremely inconvenient in the experimental process, the phenomenon of death due to large temperature difference change is easily caused during sperm thawing processing, sperm observation and subsequent experiment development are influenced, the sperm needs to be manually transferred to other processing equipment for subsequent processing after being thawed, and the sperm is easily killed due to external environment change in the transfer process, so that the sperm thawing processing device is invented to solve the problems.
Disclosure of Invention
The present invention has been made to solve the above-mentioned problems occurring in the prior art, and an object of the present invention is to provide a sperm thawing apparatus.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a sperm thawing processing apparatus, includes the thermostated container, the right flank of thermostated container is provided with feed mechanism, cell culture dish has been placed to feed mechanism's inside, the inside of thermostated container is provided with two sets of elevating system, and is two sets of elevating system front and back symmetry sets up, elevating system's surface mounting has constant temperature water bath case, constant temperature water bath case's inside is provided with adjustment mechanism, the last surface mounting of thermostated container has constant temperature control platform, thermostated container, constant temperature water bath case and constant temperature control platform's inside is linked together and sets up, constant temperature control platform's inside is provided with moving mechanism, moving mechanism's surface is provided with link gear, and moving mechanism's surface is provided with two sets of fixture, and is two sets of fixture front and back symmetry sets up, constant temperature control platform's last surface mounting has two sets of operating sleeve, and constant temperature control platform's last surface mounting has the inversion microscope, operating sleeve sets up at the right side end of inversion microscope, the left surface of constant temperature control platform has seted up the export, and the surface mounting of export has sealed lid door, the support frame is all installed at the lower surface of constant temperature control platform both ends.
Preferably, feed mechanism includes guide holder and material loading drawer, the guide holder is installed at the right flank of thermostated container and is linked together rather than inside, material loading drawer sliding connection is in the inside of thermostated container and guide holder, and the inside of material loading drawer has seted up places the hole, the cell culture dish is placed downtheholely placing.
Preferably, elevating system includes first step motor, first screw rod and first connection seat, both ends around the last inner wall of thermostated container are installed to first step motor spiro union, first screw rod passes through the shaft coupling and is connected with the power take off end transmission of first step motor, first connection seat threaded connection is on the surface of first screw rod, and first screw rod installs the front and back surface at the constant temperature water bath case.
Preferably, the supporting plates are installed at the front end and the rear end of the lower surface of the incubator, the lifting mechanism further comprises two groups of first bearing seats, the two groups of first bearing seats are sleeved on the circumferential surfaces of the upper end portion and the lower end portion of the first screw rod, and the two groups of first bearing seats are fixed on the surfaces of the inner wall of the incubator and the supporting plates.
Preferably, adjustment mechanism includes lift cylinder and lift platform, the inside packing of constant temperature water bath has the resuscitation water that unfreezes, the lift cylinder is installed in the lower inner wall middle-end of constant temperature water bath, and the output of lift cylinder sets up, lift platform installs on the output surface of lift cylinder, and the wash port has been seted up to lift platform's inside.
Preferably, moving mechanism includes second step motor, second screw rod and second connecting seat, the tip behind the right side inner wall at the thermostatic control platform is installed to the second step motor spiro union, the second screw rod is provided with two sets ofly, the rear end the second screw rod passes through the shaft coupling and is connected with the power take off end transmission of second step motor, and is two sets of the equal threaded connection in surface of second screw rod has the second connecting seat.
Preferably, the moving mechanism further comprises two sets of second bearing seats, the two sets of second bearing seats are sleeved on the circumferential surfaces of the left end portion and the right end portion of the second screw rod, and the second bearing seats are fixed on the inner wall of the constant temperature control table.
Preferably, the link gear includes driving gear, driven chain belt and driven gear, the right-hand member surface at rear end second screw rod is fixed to the driving gear, the rear end inner wall of driven chain belt meshes with the surface of driving gear mutually, driven gear is fixed on the right-hand member surface of front end second screw rod, the front end inner wall of driven chain belt meshes with driven gear's surface mutually.
Preferably, the linkage mechanism further comprises a positioning rotating rod, the positioning rotating rod is installed on the right side face of the front end second screw rod, a positioning rotating hole is formed in the inner wall of the right side of the constant temperature control table, and the positioning rotating rod is rotatably connected in the positioning rotating hole.
Preferably, fixture includes centre gripping cylinder and grip block, the centre gripping cylinder is installed on the surface of second connecting seat, and the output of two sets of centre gripping cylinders sets up towards opposite direction, the grip block is installed on the output surface of centre gripping cylinder, and the side surface of grip block and cell culture dish offsets.
The invention has the technical effects and advantages that:
1. the feeding drawer is pulled to the right side to slide out by arranging the feeding mechanism, the lifting mechanism and the constant temperature water bath box, then a thin tube containing sperms is placed inside the cell culture dish, the feeding drawer is pushed to the left side after the thin tube is placed, the cell culture dish enters the inside of the constant temperature box, at the moment, the first stepping motor is started to rotate the first screw rod, the first connecting seat drives the constant temperature water bath box to move towards the upper end, and finally the cell culture dish is soaked on the surface of water for recovery from thawing in the constant temperature water bath box, so that the sperms are thawed quickly;
2. after the sperm is unfrozen, the adjusting mechanism is arranged, the lifting cylinder is stretched and adjusted to the upper end, the lifting platform moves to the upper end and abuts against the lower surface of the cell culture dish, the cell culture dish moves to the upper end and is separated from the feeding drawer, and finally the cell culture dish enters the interior of the constant temperature control table, so that automatic transportation is realized, the whole transportation operation is carried out in the constant temperature box and the interior of the constant temperature control table, the whole transportation operation is not in contact with the external environment, and the quality of the subsequent treatment of the sperm is ensured;
3. through setting up moving mechanism, link gear and fixture, the cell culture dish rises behind the inboard of grip block, tensile regulation grip cylinder makes grip block and cell culture dish offset, the realization is fixed to the centre gripping of cell culture dish, it makes its initial position of replying to shrink regulation lift cylinder, operating personnel's hand stretches into the inside of thermostatic control platform through the operating sleeve, carry out subsequent processing to the sperm in the cell culture dish after unfreezing, after handling, it makes the second screw rod rotatory to start second step motor, make the grip block move cell culture dish left side and move, cell culture dish removes and closes second step motor behind the lower extreme of inversion microscope, carry out preliminary sperm figure aassessment through the inversion microscope, work such as sperm vitality evaluation, accomplish whole processing, moreover, the steam generator is simple in structure, and is suitable for popularization.
Drawings
FIG. 1 is a schematic view of the structure of the present invention.
FIG. 2 is a schematic front sectional view of the present invention.
FIG. 3 is a schematic view of the connection structure of the cell culture dish according to the present invention.
FIG. 4 is a schematic view of the connection structure of the lifting mechanism of the present invention.
Fig. 5 is a schematic structural diagram of the adjusting mechanism of the present invention.
FIG. 6 is a schematic view of a connection structure between the moving mechanism and the clamping mechanism.
In the figure: 1. a thermostat; 2. a feeding mechanism; 201. a guide seat; 202. a feeding drawer; 3. a cell culture dish; 4. a lifting mechanism; 401. a first stepper motor; 402. a first screw; 403. a first connecting seat; 404. a first bearing housing; 5. a constant temperature water bath tank; 6. a support plate; 7. an adjustment mechanism; 701. a lifting cylinder; 702. a lifting platform; 8. a constant temperature console; 9. a moving mechanism; 901. a second step motor; 902. a second screw; 903. a second connecting seat; 904. a second bearing housing; 10. a linkage mechanism; 1001. a driving gear; 1002. a driven chain belt; 1003. a driven gear; 1004. positioning the rotating rod; 11. a clamping mechanism; 1101. a clamping cylinder; 1102. a clamping plate; 12. an operating sleeve; 13. inverting the microscope; 14. sealing the cover door; 15. a support frame.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a sperm thawing processing device as shown in figures 1-6, which comprises a thermostat 1, wherein a feeding mechanism 2 is arranged on the right side surface of the thermostat 1, a cell culture dish 3 is placed in the feeding mechanism 2, two groups of lifting mechanisms 4 are arranged in the thermostat 1, the two groups of lifting mechanisms 4 are symmetrically arranged in the front and back direction, a constant temperature water bath box 5 is arranged on the surface of each lifting mechanism 4, an adjusting mechanism 7 is arranged in the constant temperature water bath box 5, a constant temperature control platform 8 is arranged on the upper surface of the thermostat 1, the thermostat 1 and the constant temperature water bath box 5 are communicated with the interior of the constant temperature control platform 8, a moving mechanism 9 is arranged in the constant temperature control platform 8, a linkage mechanism 10 is arranged on the surface of the moving mechanism 9, two groups of clamping mechanisms 11 are arranged on the surface of the moving mechanism 9, the two groups of clamping mechanisms 11 are symmetrically arranged in the front and back direction, two groups of operating sleeves 12 are arranged on the upper surface of the constant temperature control platform 8, an inverted microscope 13 is arranged on the upper surface of the constant temperature control platform 8, the operating sleeves 12 are arranged at the right side end of the inverted microscope 13, an outlet is provided with an outlet, two end faces of a sealed microscope support frame 14 are arranged on the left side surface of the constant temperature control platform 8, and two end faces of the microscope support frames 15 are arranged on the constant temperature control platform.
Feed mechanism 2 includes guide holder 201 and material loading drawer 202, guide holder 201 is installed at the right flank of thermostated container 1 and is linked together rather than inside, material loading drawer 202 sliding connection is in the inside of thermostated container 1 and guide holder 201, and the inside of material loading drawer 202 has been seted up and has been placed the hole, cell culture dish 3 is placed and is being placed downtheholely, pull material loading drawer 202 to the right end and take it out, then place the tubule that will hold the sperm in the inside of cell culture dish 3, promote material loading drawer 202 to the left side and make cell culture dish 3 get into the inside of thermostated container 1, then can carry out the unfreezing and subsequent processing work of sperm.
Elevating system 4 includes first step motor 401, first screw rod 402 and first connection seat 403, both ends around the last inner wall of thermostated container 1 are installed to first step motor 401 spiro union, first screw rod 402 passes through the shaft coupling and is connected with the power take off end transmission of first step motor 401, first connection seat 403 threaded connection is on the surface of first screw rod 402, and first screw rod 402 installs at the front and back surface of thermostated water bath 5, cell culture dish 3 enters into the inside back of thermostated container 1, start first step motor 401 and make first screw rod 402 rotatory, then make thermostated water bath 5 move up, make cell culture dish 3 contact with the recovery water in thermostated water bath 5, then realize the quick thawing processing to the sperm.
Backup pad 6 is all installed at both ends around the lower surface of thermostated container 1, elevating system 4 still includes first bearing frame 404, first bearing frame 404 is provided with two sets ofly, two sets of first bearing frames 404 cup joint the upper and lower both ends circumference surface at first screw rod 402, and two sets of first bearing frames 404 are fixed on the inner wall of thermostated container 1 and the surface of backup pad 6, at the rotatory in-process of adjusting first screw rod 402, first screw rod 402 is rotatory at the inside location of first bearing frame 404, avoid first screw rod 402 to take place the skew of position and angle, guarantee the stability of thermostated water bath 5 lift process.
Adjustment mechanism 7 includes lift cylinder 701 and lift platform 702, the inside packing of constant temperature water bath 5 has the water of thawing recovery, lift cylinder 701 is installed at the lower inner wall middle-end of constant temperature water bath 5, and lift cylinder 701's output sets up, lift platform 702 installs the output surface at lift cylinder 701, and lift platform 702's inside has seted up the wash port, the completion is to the back of unfreezing of sperm, upwards tensile regulation lift cylinder 701, make lift platform 702 rebound and upwards promote cell culture dish 3, cell culture dish 3 rebound breaks away from material loading drawer 202, transport cell culture dish 3 to constant temperature control platform 8 in and carry out subsequent processing.
The moving mechanism 9 comprises a second stepping motor 901, two groups of second screws 902 and a second connecting seat 903, the second stepping motor 901 is installed at the rear end part of the inner wall of the right side of the thermostatic console 8 in a threaded manner, the two groups of second screws 902 are arranged, the rear end second screw 902 is in transmission connection with the power output end of the second stepping motor 901 through a coupler, the surfaces of the two groups of second screws 902 are in threaded connection with the second connecting seat 903, after the sperms are treated through the operating sleeve 12 by the hands of an operator, the second stepping motor 901 is started to rotate the second screws 902, the position of the cell culture dish 3 is changed, the cell culture dish 3 is moved to the lower end of the inverted microscope 13, and preliminary work such as sperm number evaluation and sperm vitality evaluation is performed through the inverted microscope 13.
Moving mechanism 9 still includes second bearing frame 904, and second bearing frame 904 is provided with two sets ofly, and two sets of second bearing frames 904 cup joint at the both ends part circumference surface about second screw 902, and second bearing frame 904 fixes the inner wall at constant temperature control platform 8, and at the rotatory in-process of adjusting second screw 902, second screw 902 is rotatory at the inside location of second bearing frame 904, avoids second screw 902 to take place the skew of position and angle, guarantees the stability of cell culture dish 3 transportation.
The linkage mechanism 10 includes a driving gear 1001, a driven chain belt 1002, and a driven gear 1003, the driving gear 1001 is fixed to a right end surface of the rear second screw 902, a rear end inner wall of the driven chain belt 1002 is engaged with a surface of the driving gear 1001, the driven gear 1003 is fixed to a right end surface of the front second screw 902, a front end inner wall of the driven chain belt 1002 is engaged with a surface of the driven gear 1003, the driving gear 1001 rotates along with rotation of the second screw 902 in a process of adjusting the second stepping motor 901, the driving gear 1002 and the driven chain belt 1002 are engaged with the driven gear 1003, and then the two sets of second screws 902 rotate synchronously.
The link gear 10 still includes location bull stick 1004, location bull stick 1004 installs the right flank at front end second screw 902, the location rotating hole has been seted up on the right side inner wall of thermostatic console 8, the location bull stick 1004 rotates to be connected in the location rotating hole, at the in-process of adjusting second step motor 901, front end second screw 902 drives location bull stick 1004 rotatory in the location rotating hole when rotatory, guarantee the stability of front end second screw 902 rotation process, then guarantee the transportation quality of cell culture dish 3.
The clamping mechanism 11 comprises a clamping cylinder 1101 and a clamping plate 1102, the clamping cylinder 1101 is installed on the surface of the second connecting seat 903, the output ends of the two groups of clamping cylinders 1101 are arranged in the opposite direction, the clamping plate 1102 is installed on the surface of the output end of the clamping cylinder 1101, the clamping plate 1102 abuts against the side surface of the cell culture dish 3, after the cell culture dish 3 moves to the inner side of the clamping plate 1102, the clamping cylinder 1101 is adjusted in a stretching mode to enable the clamping plate 1102 to abut against the surface of the cell culture dish 3, clamping fixation of the cell culture dish 3 can be achieved, and the position of the cell culture dish 3 is adjusted through the matching moving mechanism 9 and the linkage mechanism 10 to perform subsequent processing operation.
The working principle is as follows: when the device is used, the feeding drawer 202 is pulled towards the right side to slide out, then a tubule containing sperms is placed in the cell culture dish 3, the feeding drawer 202 is pushed towards the left side after the tubule is placed, the cell culture dish 3 enters the inside of the constant temperature box 1, at the moment, the first stepping motor 401 is started to rotate the first screw rod 402, the first connecting seat 403 drives the constant temperature water bath box 5 to move towards the upper end, finally, the cell culture dish 3 is soaked on the surface of thawing and reviving water in the constant temperature water bath box 5 to achieve quick thawing of the sperms, after the sperms are thawed, the lifting cylinder 701 is stretched and adjusted towards the upper end, the lifting platform 702 moves towards the upper end and abuts against the lower surface of the cell culture dish 3, the cell culture dish 3 moves towards the upper end and is separated from the feeding drawer 202, finally, the cell culture dish 3 enters the inside the constant temperature control platform 8, after the cell culture dish 3 rises to the inner side of the clamping plate 1102, stretching and adjusting the clamping cylinder 1101 to enable the clamping plate 1102 to move inwards and abut against the surface of the cell culture dish 3 to clamp and fix the cell culture dish 3, after the cell culture dish 3 is fixed, contracting and adjusting the lifting cylinder 701 to enable the lifting cylinder to return to an initial position, then extending the hand of an operator into the constant temperature control table 8 through the operation sleeve 12, then performing subsequent processing on the sperm in the thawed cell culture dish 3, after the processing, starting the second stepping motor 901 to enable the second screw 902 to rotate, enabling the driving gear 1001 to be meshed with the driven chain belt 1002, enabling the driven gear 1003 to be meshed with the driven chain belt 1002 to enable the two groups of second screws 902 to synchronously rotate, then enabling the clamping plate 1102 to drive the cell culture dish 3 to move to the left side, moving the cell culture dish 3 to the lower end of the inverted microscope 13 and then closing the driving gear 1001, can carry out work such as preliminary sperm figure aassessment, sperm vigor evaluation through inverted microscope 13 this moment, accomplish this operation after, continue to start second step motor 901, make cell culture dish 3 remove to thermostatic control platform 8 the leftmost end, then open sealed cover door 14 and can take out the sperm after accomplishing the processing, whole sperm unfreeze and follow-up transportation are automatic operation, time saving and labor saving, and the operation is gone on at the inside constant temperature of thermostated container 1 and thermostatic control platform 8, the sperm is difficult for taking place to die, the sperm vitality after the thawing is high, acrosome and plasma membrane integrality can not destroyed, guarantee the subsequent use quality of sperm, moreover, the steam generator is simple in structure, and the steam generator is suitable for being generalized.

Claims (10)

1. A sperm thawing treatment device comprises an incubator (1), and is characterized in that: the cell culture dish structure is characterized in that a feeding mechanism (2) is arranged on the right side face of the incubator (1), a cell culture dish (3) is placed in the feeding mechanism (2), two sets of lifting mechanisms (4) are arranged in the incubator (1) in a front-back symmetrical mode, a constant temperature water bath box (5) is arranged on the surface of the lifting mechanism (4), an adjusting mechanism (7) is arranged in the constant temperature water bath box (5), a constant temperature control table (8) is arranged on the upper surface of the incubator (1), the constant temperature water bath box (5) and the constant temperature control table (8) are arranged in a communicated mode, a moving mechanism (9) is arranged in the constant temperature control table (8), a linkage mechanism (10) is arranged on the surface of the moving mechanism (9), two sets of clamping mechanisms (11) are arranged on the surface of the constant temperature control table (9) in a front-back symmetrical mode, two sets of clamping mechanisms (11) are arranged on the upper surface of the constant temperature control table (8), two sets of operation sleeves (12) are arranged on the constant temperature control table (8), a microscope inversion cover (13) is arranged on the left side of the microscope, and a microscope inversion cover (13) is arranged at an outlet of the microscope inversion surface, and support frames (15) are respectively installed at two end parts of the lower surface of the constant temperature control table (8).
2. A sperm cell thawing processing apparatus according to claim 1, wherein: feed mechanism (2) are including guide holder (201) and material loading drawer (202), guide holder (201) are installed at the right flank of thermostated container (1) and are linked together rather than inside, material loading drawer (202) sliding connection is in the inside of thermostated container (1) and guide holder (201), and the inside of material loading drawer (202) has seted up and has placed the hole, cell culture dish (3) are placed and are being placed downtheholely.
3. The sperm thawing processing apparatus according to claim 1, wherein: elevating system (4) include first step motor (401), first screw rod (402) and first connecting seat (403), both ends around the last inner wall of thermostated container (1) are installed to first step motor (401) spiro union, first screw rod (402) are connected through the power take off transmission of shaft coupling with first step motor (401), first connecting seat (403) threaded connection is on the surface of first screw rod (402), and first screw rod (402) are installed on the front and back surface of thermostated water bath (5).
4. The sperm cell thawing processing apparatus according to claim 3, wherein: backup pads (6) are all installed at both ends around the lower surface of thermostated container (1), elevating system (4) still include first bearing frame (404), first bearing frame (404) are provided with two sets ofly, two sets of first bearing frame (404) cup joint on the upper and lower both ends circumference surface of first screw rod (402), and the surface at the inner wall of thermostated container (1) and backup pad (6) is fixed in two sets of first bearing frame (404).
5. A sperm cell thawing processing apparatus according to claim 1, wherein: adjustment mechanism (7) are including lift cylinder (701) and lift platform (702), the inside packing of constant temperature water bath (5) has the resuscitation water of unfreezing, install in the lower inner wall middle-end of constant temperature water bath (5) lift cylinder (701), and the output of lift cylinder (701) sets up, the output surface at lift cylinder (701) is installed in lift platform (702), and the wash port has been seted up to the inside of lift platform (702).
6. A sperm cell thawing processing apparatus according to claim 1, wherein: the moving mechanism (9) comprises a second stepping motor (901), a second screw rod (902) and second connecting seats (903), the second stepping motor (901) is installed at the rear end part of the inner wall of the right side of the constant temperature control table (8) in a threaded mode, the second screw rod (902) is provided with two groups, the second screw rod (902) at the rear end is in transmission connection with the power output end of the second stepping motor (901) through a coupler, and the surfaces of the two groups of second screw rods (902) are all in threaded connection with the second connecting seats (903).
7. The sperm cell thawing processing apparatus according to claim 6, wherein: the moving mechanism (9) further comprises a second bearing seat (904), the second bearing seats (904) are provided with two groups, the second bearing seats (904) are sleeved on the circumferential surfaces of the left end portion and the right end portion of the second screw (902), and the second bearing seats (904) are fixed on the inner wall of the constant temperature control console (8).
8. The sperm thawing processing apparatus according to claim 7, wherein: the linkage mechanism (10) comprises a driving gear (1001), a driven chain belt (1002) and a driven gear (1003), wherein the driving gear (1001) is fixed on the surface of the right end portion of the rear end second screw (902), the inner wall of the rear end portion of the driven chain belt (1002) is meshed with the surface of the driving gear (1001), the driven gear (1003) is fixed on the surface of the right end portion of the front end second screw (902), and the inner wall of the front end portion of the driven chain belt (1002) is meshed with the surface of the driven gear (1003).
9. The sperm cell thawing processing apparatus according to claim 8, wherein: the linkage mechanism (10) further comprises a positioning rotating rod (1004), the positioning rotating rod (1004) is installed on the right side face of the front end second screw rod (902), a positioning rotating hole is formed in the inner wall of the right side of the constant temperature console (8), and the positioning rotating rod (1004) is rotatably connected in the positioning rotating hole.
10. A sperm cell thawing processing apparatus according to claim 9, wherein: the clamping mechanism (11) comprises clamping cylinders (1101) and clamping plates (1102), the clamping cylinders (1101) are installed on the surface of the second connecting seat (903), the output ends of the two groups of clamping cylinders (1101) are arranged in the opposite direction, the clamping plates (1102) are installed on the surfaces of the output ends of the clamping cylinders (1101), and the clamping plates (1102) abut against the side surfaces of the cell culture dish (3).
CN202211083188.1A 2022-09-06 2022-09-06 Sperm thawing treatment device Pending CN115404164A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117143704A (en) * 2023-09-11 2023-12-01 上海市生物医药技术研究院 Sperm analyzer sample preprocessing device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117143704A (en) * 2023-09-11 2023-12-01 上海市生物医药技术研究院 Sperm analyzer sample preprocessing device
CN117143704B (en) * 2023-09-11 2024-02-20 上海市生物医药技术研究院 Sperm analyzer sample preprocessing device

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