CN112369407A

CN112369407A - Cell refrigeration constant temperature test bench

Info

Publication number: CN112369407A
Application number: CN202011261506.XA
Authority: CN
Inventors: 张利
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-12
Filing date: 2020-11-12
Publication date: 2021-02-19

Abstract

The invention relates to the technical field of cell culture, and discloses a cell cold storage constant temperature test bed which comprises a workbench, a bearing, an insulation can, a clamping component, a fetching component and a base, wherein the bottom end of the inner wall of the workbench is fixedly installed with the outer wall of the bottom end of the bearing, the inner wall of the bottom end of the insulation can is fixedly installed with the inner wall of the bearing, the outer wall of the clamping component is movably connected with the inner wall of the insulation can, a culture dish is placed in the insulation can by a user, the culture dish is fixedly placed under the action of gravity of the clamping component, the insulation can is rotated to align the required culture dish with a membrane sealing table under the action of the bearing, then a loop bar is pressed to drive an arc toothed plate to rotate, and the rotation of a gear is matched with other mechanisms of the fetching component to realize the upward movement of the culture dish, so that the cell cold storage constant temperature test bed is realized, when the culture dish is taken out, the membrane is quickly sealed, and secondary infection is avoided.

Description

Cell refrigeration constant temperature test bench

Technical Field

The invention relates to the technical field of cell culture, in particular to a cell refrigeration constant-temperature test bed.

Background

The cell freezing is one of the main methods for cell preservation, the freezing technology is utilized to place the cells in liquid nitrogen with the temperature of 196 ℃ below zero for low-temperature preservation, can make the cell temporarily leave the growth state and preserve the cell characteristic, thus reviving the cell for experiment when needed, and a certain amount of cells are preserved appropriately, so that the loss of cells due to contamination or other accidents of the cells being cultured can be prevented, and the function of cell preservation is achieved, besides, some cells can be purchased, sent, exchanged and transported in a frozen form, during the passage and daily maintenance of cell culture, the culture apparatus, the culture solution and various preparation works are very costly, and once the cells leave the living body and start primary culture, various biological characteristics of the cells gradually change and are changed with the increase of the number of passages and the change of in vitro environmental conditions.

On the market at present, the cell adopts constant temperature to preserve at the in-process of cultivation mostly, this kind of preservation mode is favorable to the growth of cell, breed of other contaminated bacteria of assurance that cold-stored stage can be fine simultaneously, experimental error has been reduced, but when needs take out the culture dish, in the culture dish is gone into to the bacterium in order to avoid in the air, need seal the membrane to the culture dish outer wall and handle, traditional mode is artifical membrane that seals, this kind of mode is comparatively loaded down with trivial details, the exposure time of culture dish in the air is longer simultaneously, cause the pollution easily, consequently, need a cell cold-stored constant temperature test platform, possess when accessing the cell, the effect of quick membrane seals.

Disclosure of Invention

In order to realize the purpose of quickly sealing the membrane of the culture dish, the invention provides the following technical scheme: the cell refrigeration constant-temperature test bed comprises a workbench, a bearing, an incubator, a clamping assembly, an object-taking assembly and a base, wherein the bottom end of the inner wall of the workbench is fixedly installed with the outer wall of the bottom end of the bearing, the inner wall of the bottom end of the incubator is fixedly installed with the inner wall of the bearing, the outer wall of the clamping assembly is movably connected with the inner wall of the incubator, the outer wall of the object-taking assembly is movably connected with the inner wall of the incubator, and the inner wall of the base is movably connected with the outer wall of the object-taking assembly

As optimization, the centre gripping subassembly includes the gyro wheel, the positive fixed mounting of gyro wheel has the buckle, the outer wall swing joint of buckle has the crescent moon board, the outer wall swing joint of crescent moon board has two ball fixture blocks, the left side fixed mounting of two ball fixture blocks has the carriage release lever, the left side fixed mounting of carriage release lever has the guide pulley, the outer wall swing joint of guide pulley has the bullet rope, the top fixed mounting of bullet rope has the rope fastening, in order to realize placing the culture dish fixed, avoids it to take place to shake and influences cell culture.

As the optimization, it includes arc push pedal and arc wall to get the thing subassembly, the inner wall swing joint of arc push pedal has spherical fixture block, spherical fixture block's outer wall swing joint has the chuck, the bottom swing joint of chuck has the bull stick, the positive fixed mounting of bull stick has the movable block, the bottom swing joint of bull stick has half toothholder, the outer wall swing joint of half toothholder has the removal pinion rack, the top outer wall swing joint that removes the pinion rack has the gear, is convenient for to taking out of culture dish, avoids manual operation to cause the pollution to the culture dish.

As optimization, the gear comprises coil spring, pivot, telescopic link, arc pinion rack, loop bar, flexible spring and kelly, the inner wall of coil spring and the outer wall fixed mounting of pivot, the outer wall of pivot and the right side fixed mounting of telescopic link, the left side of telescopic link and the right side fixed mounting of arc pinion rack, the outer wall of arc pinion rack and the outer wall swing joint of loop bar, the inner wall of loop bar and flexible spring's outer wall swing joint, the inner wall of flexible spring and the outer wall swing joint of kelly, in order to realize that the culture dish is comparatively stable at the in-process that removes, avoid the shake to cause the damage to the cell in the culture dish.

Preferably, the front surfaces of the rollers are movably connected with the back surface of the crescent plate, the outer wall of the moving rod is movably connected with the inner wall of the heat preservation box, the outer wall of the rope buckle is fixedly installed with the inner wall of the heat preservation box, and the culture dish is fixed in order to realize the mutual matching of the two pairs of rollers.

Preferably, the number of the moving rods and the structures of the moving rods is eight, every two of the eight moving rods and the structures of the moving rods are symmetrically and uniformly distributed by taking the circle center of the incubator as the center, and the storage efficiency of the incubator to the culture dish is improved.

Preferably, the outer wall of the moving block is movably connected with the inner wall of the arc-shaped groove, the outer wall of the rotating rod is movably connected with the inner wall of the base, the outer wall of the coil spring is fixedly mounted with the inner wall of the gear, and the gear is driven to rotate when the coil spring rotates.

Preferably, the number of the structures of the arc-shaped push plates and the arc-shaped push plates is eight, every two structures of the eight groups of arc-shaped push plates and the arc-shaped push plates are symmetrically and uniformly distributed by taking the circle center of the heat preservation box as a pair, so that the culture dish is stable in the moving process and is prevented from colliding.

As an optimization, the quarter draw-in groove has been seted up to the left side outer wall of loop bar, and the outer wall of loop bar and the inner wall swing joint of insulation can, the left side top fixed mounting of workstation has the membrane to seal the platform, drives the arc pinion rack and rotates at the in-process that removes in order to realize the loop bar.

The invention has the beneficial effects that: this cold-stored constant temperature test bench of cell, place the culture dish in the incubator through the user, rely on under the action of gravity of centre gripping subassembly and base and self, realize the fixed of culture dish and place, when needs take out the culture dish, it makes required culture dish and membrane seal platform align to rotate the insulation can under the effect of bearing, press the rotation that the loop bar drove arc pinion rack afterwards, the normal running fit of arc pinion rack is the effect of coil spring and pivot and is making gear revolve, other mechanisms that get the thing subassembly are being cooperated again in the rotation of gear realize shifting up of culture dish, the culture dish removes and begins to seal the membrane behind the membrane seal platform, thereby realized the cold-stored constant temperature test bench of cell, seal the membrane fast when taking out the culture dish, avoid secondary infection's effect.

Drawings

FIG. 1 is a schematic diagram of the front structure of the test bed of the present invention;

FIG. 2 is a schematic cross-sectional view of a base of the test bed of the present invention;

FIG. 3 is an enlarged view of a portion of FIG. 1 at A;

FIG. 4 is a schematic cross-sectional view of a loop bar of a test bed according to the present invention;

FIG. 5 is a schematic top view of the test stand of the present invention.

In the figure: 1. a work table; 2. a bearing; 3. a heat preservation box; 4. a clamping assembly; 401. a roller; 402. buckling; 403. a crescent plate; 404. double ball fixture blocks; 405. a travel bar; 406. a guide wheel; 407. a rope bouncing; 408. a rope fastener; 5. a fetching component; 501. an arc-shaped push plate; 502. a spherical fixture block; 503. a chuck; 504. a rotating rod; 505. a moving block; 506. an arc-shaped slot; 507. a half-toothholder; 508. a movable toothed plate; 509. a gear; 5091. a coil spring; 5092. a rotating shaft; 5093. a telescopic rod; 5094. an arc toothed plate; 5095. a loop bar; 5096. a flexible spring; 5097. a clamping rod; 6. a base.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, a cell cold storage constant temperature test bench comprises a workbench 1, a bearing 2, an incubator 3, a clamping component 4, a fetching component 5 and a base 6, wherein the bottom end of the inner wall of the workbench 1 is fixedly installed with the outer wall of the bottom end of the bearing 2, the inner wall of the bottom end of the incubator 3 is fixedly installed with the inner wall of the bearing 2, the outer wall of the clamping component 4 is movably connected with the inner wall of the incubator 3, the outer wall of the fetching component 5 is movably connected with the inner wall of the incubator 3, the inner wall of the base 6 is movably connected with the outer wall of the fetching component 5, the clamping component 4 comprises a roller 401, the front surface of the roller 401 is movably connected with the back surface of a crescent plate 403, the outer wall of a movable rod 405 is movably connected with the inner wall of the incubator 3, the outer wall of a rope buckle 408 is fixedly installed with the inner wall of the incubator 3, the outer wall of the buckle 402 is movably connected with a crescent 403, the outer wall of the crescent 403 is movably connected with a double-ball fixture block 404, the left side of the double-ball fixture block 404 is fixedly provided with a moving rod 405, the number of the moving rod 405 and the moving rod 405 is eight, the eight moving rod 405 and the moving rod 405 are in pair, the moving rod 405 and the moving rod 405 are symmetrically and uniformly distributed by taking the center of a circle of the incubator 3 as the center, in order to improve the storage efficiency of the incubator 3 on a culture dish, the left side of the moving rod 405 is fixedly provided with a guide wheel 406, the outer wall of the guide wheel 406 is movably connected with an elastic rope 407, the top end of the elastic rope 407 is fixedly provided with a rope buckle 408, in order to realize the fixation when the culture dish is placed and avoid the shake to influence on cell culture, the fetching component 5 comprises an arc push plate 501 and an arc groove 506, the number of the structures of the arc push plate 501 and the arc push plate 501 are eight, the structures of the eight, in order to realize that the culture dish is relatively stable in the moving process and avoid collision, the inner wall of the arc-shaped push plate 501 is movably connected with a spherical fixture block 502, the outer wall of the spherical fixture block 502 is movably connected with a chuck 503, the bottom end of the chuck 503 is movably connected with a rotating rod 504, the front surface of the rotating rod 504 is fixedly provided with a moving block 505, the outer wall of the moving block 505 is movably connected with the inner wall of the arc-shaped groove 506, the outer wall of the rotating rod 504 is movably connected with the inner wall of the base 6, the outer wall of a coil spring 5091 is fixedly arranged with the inner wall of a gear 509, in order to realize that the coil spring 5091 drives the gear 509 to rotate when rotating, the bottom end of the rotating rod 504 is movably connected with a half-tooth seat 507, the outer wall of the half-tooth seat 507 is movably connected with a moving toothed plate 508, the outer wall of the top end of the moving toothed plate 508, The telescopic rod 5093, the arc toothed plate 5094, the loop bar 5095, the flexible spring 5096 and the clamping rod 5097 are combined, the inner wall of the coil spring 5091 is fixedly installed with the outer wall of the rotating shaft 5092, the outer wall of the rotating shaft 5092 is fixedly installed with the right side of the telescopic rod 5093, the left side of the telescopic rod 5093 is fixedly installed with the right side of the arc toothed plate 5094, the outer wall of the arc toothed plate 5094 is movably connected with the outer wall of the loop bar 5095, the outer wall of the left side of the loop bar 5095 is provided with a quarter clamping groove, the outer wall of the loop bar 5095 is movably connected with the inner wall of the heat preservation box 3, the top end of the left side of the workbench 1 is fixedly provided with a membrane sealing platform, in order to realize that the loop bar 5095 drives the arc-shaped toothed plate 5094 to rotate in the moving process, the inner wall of the loop bar 5095 is movably connected with the outer wall of the flexible spring 5096, the inner wall of the flexible spring 5096 is movably connected with the outer wall of the clamping rod 5097, in order to realize that the culture dish is comparatively stable at the in-process that removes, avoid the shake to cause the damage to the cell in the culture dish.

When in use, please refer to fig. 1-5, a user places a culture dish in the incubator 3, the outer shell of the culture dish extrudes the roller 401, the roller 401 moves to drive the buckle 402 to move, the buckle 402 moves to drive the crescent 403 to move, the crescent 403 moves to drive the double-ball fixture block 404 to move, the double-ball fixture block 404 moves to drive the moving rod 405 to move, the moving rod 405 drives the guide wheel 406, the guide wheel 406 causes the elastic rope 407 to stretch, the elastic rope 407 drives the roller 401 to clamp the culture dish in the process of recovering elastic deformation, when the culture dish needs to be taken out, the incubator 3 is rotated to align the required culture dish with the membrane sealing platform under the action of the bearing 2, then the loop bar 5095 is pressed to move, the movement of the loop bar 5095 is limited by the clamp bar 5097 and simultaneously extrudes the flexible spring 5096 and drives the arc toothed plate 5094 to rotate, the arc toothed plate 5094 rotates to drive the telescopic rod 5093 to rotate, rotation of telescopic link 5093 drives the rotation of pivot 5092, pivot 5092 rotates and makes coil spring 5091 curl and drive gear 509 and rotate simultaneously, gear 509's rotation is driving the removal of moving toothed plate 508, the removal of moving toothed plate 508 is cooperating the rotation that half toothholder 507 realized bull stick 504, the rotation of bull stick 504 is cooperating spherical fixture block 502 and arc push pedal 501's effect again and is realizing the removal of culture dish in incubator 3, the culture dish removes and begins to seal the membrane after sealing the platform to the membrane, thereby realized the cold-stored constant temperature test bench of cell, seal the membrane fast when taking out the culture dish, avoid the effect that the air exposes the infection.

To sum up, this kind of cold-stored constant temperature test bench of cell, place the culture dish in incubator 3 through the user, rely on clamping component 4 and under the action of gravity of base 6 and self, realize the fixed of culture dish and place, when needs take out the culture dish, it makes required culture dish and membrane seal platform align to rotate insulation box 3 under bearing 2's effect, press loop bar 5095 to drive arc pinion 5094's rotation afterwards, arc pinion 5094's normal running fit coil spring 5091 and pivot 5092's effect makes gear 509 rotate, gear 509's rotation is cooperating other mechanisms of getting thing subassembly 5 again and is realizing shifting up of culture dish, the culture dish removes and begins to seal the membrane after the membrane seals the platform, thereby realized the cold-stored constant temperature test bench of cell, seal the membrane fast when taking out the culture dish, avoid secondary infection's effect.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.

Claims

1. The utility model provides a cell refrigeration constant temperature test bench, includes workstation (1), bearing (2), insulation can (3), centre gripping subassembly (4), gets thing subassembly (5) and base (6), its characterized in that: the inner wall bottom of workstation (1) and the bottom outer wall fixed mounting of bearing (2), the bottom inner wall of insulation can (3) and the inner wall fixed mounting of bearing (2), the outer wall of centre gripping subassembly (4) and the inner wall swing joint of insulation can (3), get the outer wall of thing subassembly (5) and the inner wall swing joint of insulation can (3), the inner wall of base (6) and the outer wall swing joint of getting thing subassembly (5).

2. A cell cold storage constant temperature test bench according to claim 1, characterized in that: the clamping assembly (4) comprises a roller (401), a buckle (402) is fixedly mounted on the front face of the roller (401), a crescent-shaped plate (403) is movably connected to the outer wall of the buckle (402), a double-ball fixture block (404) is movably connected to the outer wall of the crescent-shaped plate (403), a moving rod (405) is fixedly mounted on the left side of the double-ball fixture block (404), a guide wheel (406) is fixedly mounted on the left side of the moving rod (405), an elastic rope (407) is movably connected to the outer wall of the guide wheel (406), and a rope buckle (408) is fixedly mounted on the top end of the elastic rope (407).

3. A cell cold storage constant temperature test bench according to claim 1, characterized in that: get thing subassembly (5) and include arc push pedal (501) and arc groove (506), the inner wall swing joint of arc push pedal (501) has spherical fixture block (502), the outer wall swing joint of spherical fixture block (502) has chuck (503), the bottom swing joint of chuck (503) has bull stick (504), the positive fixed mounting of bull stick (504) has movable block (505), the bottom swing joint of bull stick (504) has half toothholder (507), the outer wall swing joint of half toothholder (507) has removal pinion rack (508), the top outer wall swing joint of removing pinion rack (508) has gear (509).

4. A cell cold storage constant temperature test bench according to claim 3, characterized in that: gear (509) comprise wind spring (5091), pivot (5092), telescopic link (5093), arc pinion rack (5094), loop bar (5095), flexible spring (5096) and pole (5097), the inner wall of wind spring (5091) and the outer wall fixed mounting of pivot (5092), the outer wall of pivot (5092) and the right side fixed mounting of telescopic link (5093), the left side of telescopic link (5093) and the right side fixed mounting of arc pinion rack (5094), the outer wall of arc pinion rack (5094) and the outer wall swing joint of loop bar (5095), the inner wall of loop bar (5095) and the outer wall swing joint of flexible spring (5096), the inner wall of flexible spring (5096) and the outer wall swing joint of kelly (5097).

5. A cell cold storage constant temperature test bench according to claim 2, characterized in that: the front surface of the roller (401) is movably connected with the back surface of the crescent plate (403), the outer wall of the moving rod (405) is movably connected with the inner wall of the heat preservation box (3), and the outer wall of the rope buckle (408) is fixedly installed with the inner wall of the heat preservation box (3).

6. A cell cold storage constant temperature test bench according to claim 2, characterized in that: the number of the structures of the moving rods (405) and the moving rods (405) is eight, and every two of the structures of the eight moving rods (405) and the moving rods (405) are symmetrically and uniformly distributed by taking the circle center of the heat preservation box (3) as the center.

7. A cell cold storage constant temperature test bench according to claim 3, characterized in that: the outer wall of the moving block (505) is movably connected with the inner wall of the arc-shaped groove (506), the outer wall of the rotating rod (504) is movably connected with the inner wall of the base (6), and the outer wall of the coil spring (5091) is fixedly installed with the inner wall of the gear (509).

8. A cell cold storage constant temperature test bench according to claim 3, characterized in that: the number of the structures of the arc-shaped push plates (501) and the arc-shaped push plates (501) is eight, and the structures of the eight groups of arc-shaped push plates (501) and the arc-shaped push plates (501) are pairwise and are symmetrically and uniformly distributed by taking the circle center of the heat insulation box (3) as the center.

9. A cell cold storage constant temperature test bench according to claim 4, characterized in that: the clamping groove of one fourth is seted up to the left side outer wall of loop bar (5095), and the outer wall of loop bar (5095) and the inner wall swing joint of insulation can (3), the left side top fixed mounting of workstation (1) has the membrane to seal the platform.