CN115404136A - Natural environment-friendly cleaning agent for aquatic products, method and application - Google Patents
Natural environment-friendly cleaning agent for aquatic products, method and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/40—Products in which the composition is not well defined
- C11D7/44—Vegetable products
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- A—HUMAN NECESSITIES
- A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
- A22C—PROCESSING MEAT, POULTRY, OR FISH
- A22C29/00—Processing shellfish or bivalves, e.g. oysters, lobsters; Devices therefor, e.g. claw locks, claw crushers, grading devices; Processing lines
- A22C29/02—Processing shrimps, lobsters or the like ; Methods or machines for the shelling of shellfish
- A22C29/021—Cleaning operations on shellfish, e.g. evisceration, brushing
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- C11D2111/14—
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a natural environment-friendly cleaning agent for aquatic products, a method and application thereof, wherein the natural environment-friendly cleaning agent for aquatic products comprises the following components: the weight ratio of the green lemon extract to the cloves extract is 1:0-5. The invention also provides a preparation method of the natural environment-friendly cleaning agent for the aquatic products and application of the natural environment-friendly cleaning agent for the aquatic products in cleaning crayfishes. The aquatic product natural environment-friendly cleaning agent provided by the invention adopts natural food source extracts, is a novel compound biological environment-friendly crawfish cleaning agent with sterilization and bacteriostasis characteristics, and is safe for eating and driving protection of crawfishes.
Description
Technical Field
The invention relates to the field of aquatic product cleaning, and in particular relates to a natural environment-friendly cleaning agent for aquatic products, a method and application thereof.
Background
Crayfish, the academic name procambarus clarkii, is an important freshwater economic shrimp. In recent years, the crayfish industry has been rapidly developed, and crayfish prefers to live in environments such as ditches, aquaculture ponds, and rice fields, and mainly eats animal foods such as plankton, underwater fallen algae, insect bodies, earthworms, snails, and clams. Because the breeding environment of crayfish has more problems, the pollution of the breeding water body, such as: the water eutrophication causes the growth of toxic algae, the severe fluctuation of dissolved oxygen in the water, the enrichment of heavy metals, the pollution of other toxic substances (such as chloride, arsenide, nitrite and the like), the change of hydration factors and the like, so that the water is deteriorated, a large amount of pathogenic bacteria are bred, a large amount of pathogenic microorganisms such as viruses and bacteria, parasites, heavy metals, veterinary drug residues and the like are bred in the water and spread all around, and then the pathogenic microorganisms are attached to the body surface of the crayfish.
At present, the dirt on the body surface of the crayfish is generally cleaned by combining shrimp washing powder with ultrasonic treatment, the shrimp washing powder on the market mainly comprises citric acid, and the citric acid has no direct harm to human bodies, but can promote the excretion and deposition of calcium in the bodies, and if the crayfish is eaten for a long time, the food containing the citric acid can possibly cause hypocalcemia. Therefore, the development of a biological environment-friendly crayfish cleaning agent capable of replacing chemically synthesized shrimp washing powder is a very urgent subject.
At present, chemical cleaning agents are mostly adopted for cleaning crayfishes. The invention patent of China with the application number of 200910051437.7 adopts salt, sodium carbonate and sodium bicarbonate to clean crayfish. The Chinese invention patent with the application number of CN110754509A adopts 10ppm low-chlorine solution for cleaning and sterilization. The Chinese invention patent with the application number of CN110938501A adopts protease, cellulase, amylase, lipase and lysozyme to clean crayfish. However, so far, no report about a natural extract cleaning agent for crayfish is found.
Disclosure of Invention
The embodiment of the invention provides a natural environment-friendly cleaning agent for aquatic products, which adopts natural food source extracts and is a novel compound biological environment-friendly cleaning agent for aquatic products (such as crayfish) with sterilization and bacteriostasis characteristics, and is safe for eating and driving of aquatic products.
The embodiment of the invention provides a natural environment-friendly cleaning agent for aquatic products, which comprises: the weight ratio of the green lemon extract to the cloves extract is 1:0-5. In the invention, the lime extract and the flos caryophylli extract are natural extracts, and the extracts can be used for cleaning crayfish well and have the characteristics of sterilization and bacteriostasis.
According to the natural environment-friendly cleaning agent for aquatic products, provided by the embodiment of the invention, the weight ratio of the lime extract to the cloves extract is 1:2-3, preferably 1:2. According to the invention, by adopting the proportion of the lime extract and the flos caryophyllata extract, the abdominal brightness of the crayfish can be increased, and the cleaning effect is better.
The natural environment-friendly cleaning agent for aquatic products provided by the embodiment of the invention is composed of a lime extract and a flos caryophylli extract.
According to the natural environment-friendly cleaning agent for aquatic products, provided by the embodiment of the invention, the lime extract is powder with the particle size of 0.2-1 mm, and the clove extract is powder with the particle size of 0.2-0.7 mm.
According to the preparation method of the natural environment-friendly cleaning agent for the aquatic products, provided by the embodiment of the invention, a green lemon raw material and a flos caryophyllata raw material are dried to obtain a green lemon crude product and a flos caryophyllata crude product, and the green lemon crude product and the flos caryophyllata crude product are mixed in proportion and then crushed to obtain the natural environment-friendly cleaning agent for the aquatic products; or drying the green lemon raw material to obtain a green lemon crude product, then crushing to obtain a green lemon extract, and then mixing the green lemon extract and the cloves extract in proportion to obtain the natural environment-friendly cleaning agent for the aquatic products; preferably, the lime raw material is freshly squeezed lime; the drying temperature is 5-45 ℃, and room temperature drying is preferred. According to the invention, the shelf life of the cleaning agent can be prolonged by adopting the drying preparation method of the natural environment-friendly cleaning agent for aquatic products; the cleaning components of the product can be dissolved out more easily, so that the cleaning effect of the crayfish is improved.
The embodiment of the invention also provides application of the natural environment-friendly cleaning agent for aquatic products in cleaning crayfishes.
According to the application of the natural environment-friendly cleaning agent for the aquatic products in cleaning the crayfishes, the natural environment-friendly cleaning agent for the aquatic products and clear water are mixed in proportion to obtain a cleaning solution, and the cleaning solution is used for cleaning the crayfishes.
According to the application of the natural environment-friendly cleaning agent for the aquatic products in cleaning the crayfish, which is provided by the embodiment of the invention, the mass-to-volume ratio of the natural environment-friendly cleaning agent for the aquatic products to clear water is 20-50 g:1.5L, preferably 30g:1.5L. According to the invention, the cleaning effect of the crayfish can be improved by adopting the preferable proportion of the natural environment-friendly cleaning agent and the clear water.
According to the application of the natural environment-friendly cleaning agent for aquatic products in cleaning crayfishes, the cleaning solution is used for cleaning crayfishes under ultrasonic treatment, the cleaning temperature is 15-25 ℃, 20 ℃ is preferred, and the cleaning time is 3-8 min, 5min is preferred; the conditions of the ultrasonic treatment are preferably 15KW/L. In the invention, under the ultrasonic treatment condition, the cleaning effect of the crayfish can be improved.
The invention has the beneficial effects that: the natural environment-friendly crawfish cleaning agent is developed for crawfish, selects the natural extract as the cleaning agent component, has the characteristics of sterilization and bacteriostasis, greatly improves the surface cleanliness, the sensory quality and the edible safety of crawfish, and has great prospect in expanding the sales market of crawfish, improving the selling quality of fresh crawfish and improving the cooking and edible safety. The crayfish surface cleanliness is evaluated by adopting an image analysis method for the first time.
Drawings
In order to more clearly illustrate the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a comparison diagram of the abdomen of crayfishes before and after cleaning with cleaning agents of different compounding ratios; before cleaning, the step (a) and after cleaning, the step (b);
FIG. 2 is a graph of image RGB value to LAB value code in the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The instruments and the like are conventional products which are purchased by normal distributors and are not indicated by manufacturers. The process is conventional unless otherwise specified, and the starting materials are commercially available from the open literature. The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications.
In the following examples of the present invention, (one) colony count was determined: refer to the plate counting method of GB4789.2-2016 assay. Dividing 6 crayfishes before and after cleaning into three groups, one group comprises 2 crayfishes with the serial numbers of 12, 34 and 56, filling the crayfishes into a sterile beaker, adding 350mL of sterile water to ensure that the crayfishes do not exist, fully oscillating the crayfishes for 5min at room temperature through a DSHZ-300A water bath constant temperature oscillator, taking liquid containing crayfish body surface bacteria through sterile operation, and mixing the liquid with normal saline to prepare 1:10, three suitable dilutions were selected and plated on PCA media to culture colonies for determination of colony count. (II) LAB image analysis: the method comprises the steps of building a black studio, adjusting light, angles and other environmental factors to be consistent, fixing the crayfishes before and after cleaning in the center of the studio, taking pictures by using a professional camera, intercepting the belly of each group of crayfishes before and after cleaning, writing codes by using R language analysis software to perform RGB professional technical analysis, taking RGB average values of all pixel points (the codes are shown in figure 2), converting the RGB average values into LAB values by using an RGB color conversion tool, and analyzing and comparing changes of brightness values (L) before and after cleaning. (III) sensory evaluation: making a sensory evaluation table and determining a evaluation standard, wherein in the research of the cleaning effect of the lime extract, a reviewer is asked to evaluate the quality of the crayfish before and after cleaning from the aspects of color, smell, muscle tissue, appearance, hand feeling and overall acceptability of 6, and the specific evaluation standard is shown in table 1. In the research of the compound cleaning effect of the lime extract and the flos caryophyllata extract, photos of 6 crayfishes before and after cleaning are selected by random numbers, questionnaires are made in the aspects of appearance, color and overall acceptability being 3, and the evaluation results of evaluation personnel at all age stages are collected for analysis and comparison.
TABLE 1 crayfish organoleptic Property test Scoring criteria
In SB/T108760-2012, sensory standards of freshwater first-class crayfish require that the appearance of crayfish is bright and the shell is glossy. The high-density crayfish breeding characteristic makes the water area self-cleaning ability worsen, causes a large amount of pathogenic microorganisms such as virus, bacterium to breed in the water and attached to the crayfish body surface, and effective degerming is favorable to the security of crayfish in transportation, sale and follow-up processing stage. Sensory evaluation results before and after cleaning of crayfish in the following three examples show that the overall acceptability of consumers to crayfish is increased by 439.96% after cleaning, and the degerming rate is more than 85%.
In the following examples of the present invention, the (public) clove extract is a clove powder, which is purchased from Hongbaotang commercial Co., ltd, in Bozhou city, and has a particle size of 0.2 to 0.7mm, and the lime is purchased from Kangning agricultural development Co., ltd, in Guangxi Teng county, and the extract is prepared in a laboratory, and has a particle size of 0.2 to 1mm. The KQ3200DE type numerical control ultrasonic cleaner is purchased from ultrasonic instruments Inc. of Kunshan city.
Example 1
According to the technical scheme, the green lemon extract comprises the following components: flos Caryophylli extract =1:3, freshly squeezed green lemon (peeled) is dried and compounded with flos Caryophylli powder, 30g of the compounded product is weighed, mixed and dissolved in 1.5L of deionized water, stirred uniformly, and the pH is measured to be 3.64. And adding the prepared cleaning liquid into an ultrasonic cleaner, and cleaning the crayfish for 5min at 20 ℃ and 15KW/L. Photographing the crayfish before and after cleaning (as shown in figure 1), carrying out comparative analysis on an L value (brightness value) through an image Lab channel, carrying out sensory evaluation before and after cleaning, and finally detecting the colony count of the crayfish before and after cleaning according to the GB4789.2-2016 method. The results are obtained in table 2:
TABLE 2 Cray cleaning Effect measurement results
Example 2
According to the technical scheme, the green lemon extract comprises the following components: the mass ratio of the flos caryophyllata extract =1:2, fresh squeezed green lemon is dried and compounded with flos caryophyllata powder, 30g of the compounded product is weighed, mixed and dissolved into 1.5L of deionized water, stirred uniformly, and the pH value is measured to be 3.56. Adding the prepared cleaning solution into an ultrasonic cleaner, and cleaning the crayfish for 5min at 20 ℃ and 100% power. Photographing the crayfish before and after cleaning (as shown in figure 1), carrying out comparative analysis on an L value (brightness value) through an image Lab channel, carrying out sensory evaluation before and after cleaning, and finally detecting the colony count of the cleaned crayfish according to the GB4789.2-2016 method. The results are obtained in table 3:
TABLE 3 Cray cleaning Effect measurement results
Comparative example 1
Citric acid (2-hydroxy-tricarballylic acid) was dissolved in 1.5L of deionized water in portions, stirred uniformly, and the addition was stopped after the pH was adjusted to 3.56. Adding the prepared cleaning solution into an ultrasonic cleaner, and cleaning the crayfish for 5min at 20 ℃ and 100% power. Photographing the crayfish before and after cleaning (as shown in figure 1), carrying out comparative analysis on an L value (brightness value) through an image Lab channel, carrying out sensory evaluation before and after cleaning, and finally detecting the colony count of the cleaned crayfish according to the GB4789.2-2016 method. The results are obtained in table 4:
TABLE 4 crayfish cleaning Effect measurement results
Experimental example 1
Cleaning effect study of lime extract: one total of four treatment groups: clear water, lime extract, synthetic citric acid reagent, 6 crayfish per group, numbered. Cleaning each treatment group for 3min and 5min, respectively, preparing 1.5L deionized water (or dissolving 20g of green lemon extract in 1.5L deionized water, or dissolving about 1.44g of industrial citric acid reagent in 1.5L deionized water, stirring well), measuring pH to make pH of the three groups of non-peeled, peeled and citric acid basically consistent (about 2.7), adding into KQ3200DE type numerical control ultrasonic cleaner for cleaning, and sequentially photographing crayfish before cleaning, 3min cleaning and 5min cleaning after cleaning. In the cleaning effect research process of the step1 lime extract, the best effect of the peeled lime extract is obtained through detection index results such as colony count, image analysis, sensory evaluation, heavy metal detection and the like.
Experimental example 2
The cleaning effect research of the compound of the lime extract and the flos caryophyllata extract comprises the following steps: a total of six treatment groups: lime extract-cloves extract mass ratio =5, 1,1. Cleaning each treatment group for 5min, mixing and dissolving the green lemon extract and the flos Caryophylli extract in 1.5L deionized water, stirring, measuring pH (about 3.5), cleaning in a KQ3200DE type numerical control ultrasonic cleaner, and sequentially photographing the crayfish before and for 5min after cleaning. Further, in the research process of the cleaning effect of the step2 lime extract and the cloves extract, the best cleaning effect of the lime 1: cloves 2 compound group on crayfish is obtained through detection index results such as colony count, image analysis, sensory evaluation, heavy metal detection and the like.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. An aquatic product natural environment-friendly cleaning agent is characterized by comprising: the weight ratio of the green lemon extract to the cloves extract is 1:0-5.
2. The aquatic product natural environment-friendly cleaning agent as claimed in claim 1, wherein the weight ratio of the lime extract to the cloves extract is 1:2-3, preferably 1:2.
3. The aquatic product natural environment-friendly cleaning agent as claimed in claim 2, which is composed of a lime extract and a flos caryophylli extract.
4. The aquatic product natural environment-friendly cleaning agent as claimed in any one of claims 1 to 3, wherein the lime extract is powder with a particle size of 0.2-1 mm, and the flos caryophylli extract is powder with a particle size of 0.2-0.7 mm.
5. A preparation method of the natural environment-friendly aquatic product cleaning agent as described in any one of claims 1 to 3, comprising: drying a green lemon raw material and a flos caryophyllata raw material to obtain a green lemon crude product and a flos caryophyllata crude product, mixing the green lemon crude product and the flos caryophyllata crude product in proportion, and crushing to obtain the natural environment-friendly cleaning agent for the aquatic products; or drying the green lemon raw material to obtain a green lemon crude product, then crushing to obtain a green lemon extract, and then mixing the green lemon extract and the cloves extract in proportion to obtain the natural environment-friendly cleaning agent for the aquatic products; preferably, the green lemon raw material is freshly squeezed green lemon; the drying temperature is 5-45 ℃, and room temperature drying is preferred.
6. The use of the aquatic product natural environment-friendly cleaning agent as defined in any one of claims 1-4 for cleaning crayfish.
7. The application of the aquatic product natural environment-friendly cleaning agent in cleaning crayfishes as claimed in claim 6, wherein the aquatic product natural environment-friendly cleaning agent is mixed with clear water in proportion to obtain a cleaning solution, and the crayfishes are cleaned by the cleaning solution.
8. The application of the aquatic product natural environment-friendly cleaning agent in cleaning crayfishes as claimed in claim 7, wherein the mass-to-volume ratio of the aquatic product natural environment-friendly cleaning agent to clear water is 20-50 g:1.5L, preferably 30g:1.5L.
9. The application of the aquatic product natural environment-friendly cleaning agent in cleaning crayfishes as claimed in claim 8, wherein the cleaning solution is used for cleaning crayfishes under ultrasonic treatment, the cleaning temperature is 15-25 ℃, preferably 20 ℃, and the cleaning time is 3-8 min, preferably 5min; the conditions of the ultrasonic treatment are preferably 15KW/L.
10. A method for evaluating the surface cleanliness of crayfishes, which is characterized in that the image analysis method is adopted to evaluate the surface cleanliness of the crayfishes cleaned by the aquatic product natural environment-friendly cleaning agent according to any one of claims 1 to 4.
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