CN115389497A

CN115389497A - Sample analysis system, sample image capturing method, and computer-readable storage medium

Info

Publication number: CN115389497A
Application number: CN202110546412.5A
Authority: CN
Inventors: 叶波; 罗玮; 余珊; 陈巧妮
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2021-05-19
Filing date: 2021-05-19
Publication date: 2022-11-25

Abstract

The invention discloses a sample analysis system, a sample image photographing method and a computer-readable storage medium. The sample analysis system includes a sample image capture device configured to capture a sample to be tested on a sample to be tested carrier, and a controller. The controller is in communication connection with the sample image shooting device and is configured to acquire a first working area of the sample carrier to be detected and a preset number of target cells, when the number of the target cells in the first working area is judged to be smaller than the preset number, the first working area is adjusted to form a second working area, and the sample image shooting device is controlled to shoot the second working area, so that a cell image containing the preset number of the target cells can be acquired. The invention can dynamically adjust the counting area, and ensure that a cell image containing a preset number of target cells can be obtained.

Description

Sample analysis system, sample image capturing method, and computer-readable storage medium

Technical Field

The present invention relates to the field of sample image capturing, and in particular, to a sample analysis system, a sample image capturing method, and a computer-readable storage medium.

Background

A slide reader (generally called a cytomorphological analyzer or a blood cell digital image system) is an instrument for analyzing cells on smears of peripheral blood, bone marrow, body fluids, and the like. For example, a blood cell digital image system can shoot a blood smear coated with a blood film and display the shot blood cell image to a user, which can replace the manual microscopic examination work to some extent.

The main working process and the working principle of the piece reading machine are as follows: based on a microscopic optical and digital shooting module, an intelligent image processing algorithm is applied to automatically identify a monolayer cell area, blood cells (white blood cells, red blood cells, platelets and the like) are searched and shot in the monolayer cell area, the shot images are subjected to necessary image processing, the types, the number and the characteristics of the shot cells are identified through the intelligent identification algorithm, and the cells are classified and displayed on a display according to the characteristics of the cells. The operation user can adjust the instrument classification result according to clinical experience and patient related information and give corresponding clinical conclusion.

In the related art, when counting leukocytes, the reader generally determines a fixed working area on a blood membrane, and then photographs and counts the leukocytes in the fixed working area, so as to obtain the clinically required number of leukocytes. However, for abnormal samples with low white blood cell values, a sufficient number of white blood cells may not be located by the above counting method.

It is important to note here that the statements in this background section merely provide background information related to the present disclosure and may not necessarily constitute prior art.

Disclosure of Invention

The object of the present invention is to provide a sample analysis system, a sample image capturing method, and a computer-readable storage medium that ensure that a sufficient number of target cells can be captured by dynamically adjusting a working area or a counting area.

To this end, a first aspect of the invention provides a sample analysis system comprising:

a sample image shooting device configured to shoot a sample to be detected on a sample carrier to be detected to obtain a cell image; and

a controller communicatively connected with the sample image capture device and configured to:

acquiring a first working area of a sample carrier to be detected and a preset number of target cells,

and when the number of the target cells in the first working area is judged to be less than the preset number, adjusting the first working area to form a second working area, and controlling the sample image shooting device to shoot the second working area so as to obtain a cell image containing the preset number of the target cells.

In embodiments of the present invention, the first working area is typically a working area that is pre-set with dimensional parameters (e.g., fixed length, fixed width, or fixed area). The size parameters of the first working area may be different for different samples. Under normal conditions, the number of target cells required for clinical positioning can be scanned by photographing the first working area, that is, a preset number of target cells can be photographed in the first working area. When the sample analysis system recognizes that a predetermined number of target cells may not be available even though the entire first working area is photographed, the first working area is adjusted. And the sample image shooting device shoots the adjusted first working area, and analyzes the shot cell image so as to identify or locate the preset number of target cells.

Preferably, before determining that the number of target cells possibly existing in the first working area is less than the preset number, the controller is configured to control the sample image capturing device to capture a partial area of the first working area to obtain the first cell image.

In some embodiments, the cell image for obtaining the preset number of target cells may include the first cell image that has been captured and the second cell image that is captured for the second working area, or may include only the second cell image that is captured for the second working area.

In some embodiments, the controller may be further configured to: and judging whether the number of the target cells in the first working area is less than a preset number or not according to the shot first cell image. That is, the number of target cells in the photographed area of the first working area is calculated from the first cell image that has been photographed; then, estimating the estimated number of target cells possibly contained in the whole first working area according to the target number in the shot area; comparing the estimated quantity with a preset target quantity; if the estimated number is smaller than the preset target number, the first working area is adjusted, especially enlarged.

In some embodiments, the controller is further configured to: when the sample image shooting device is controlled to shoot the first working area, no matter whether the sample image shooting device finishes shooting the first working area or not, when the number of the target cells in the first working area is judged to be smaller than the preset number, the first working area is adjusted in real time to form a second working area. That is, during the photographing of the first working area, the first working area is adjusted in real time according to the determination result, so that a preset number of target cells can be photographed as fast as possible.

In some embodiments, the controller may be further configured to: and determining a second working area according to the number of the target cells obtained by shooting and the difference value between the preset numbers.

In some alternative or additional embodiments, the controller may be further configured to: and acquiring a cell detection result of the blood analysis device on the sample to be detected, such as a blood routine detection result, and judging whether the number of the target cells in the first working area is less than a preset number according to the cell detection result. For example, if the white blood cell count obtained by the blood analysis device is less than a predetermined threshold value when the target cells are white blood cells, it can be concluded that the number of white blood cells in the first working area is less than the predetermined number, wherein the first working area is adjusted, in particular enlarged, according to the invention.

In some alternative embodiments, the controller may be further configured to: and acquiring a cell detection result of the blood analysis device on the sample to be detected, and judging whether the number of the target cells in the first working area is less than a preset number or not according to the cell detection result and the shot first cell image.

In some embodiments, the controller may be further configured to: and judging that the number of the target cells in the first working area is less than the preset number according to the target cell low value abnormality of the sample to be detected and/or the first working area bias. Here, whether or not there is an abnormality of a low value of the target cell in the sample to be tested can be determined from the first cell image which has been captured or the cell detection result of the blood analyzer. In addition, whether the phenomenon of the first working area offset exists can be judged according to the first cell image obtained by shooting or the appearance image of the sample to be detected on the sample carrier to be detected.

In some embodiments, the second working area may comprise at least a partial area of the first working area; or the second working area may be independent of the first working area.

In some embodiments, the controller may be further configured to: when the first working area is adjusted to form the second working area, the first working area is enlarged on the basis of the first working area along the longitudinal direction and/or the transverse direction of the sample carrier to be measured to form the second working area. This is particularly advantageous when low-value abnormalities of target cells are present in the test sample.

In some embodiments, the target cell may be a white blood cell or a red blood cell.

In some embodiments, the controller may be further configured to: and outputting a prompt whether to shoot in the non-working area when the number of the target cells in the second working area is judged to be less than the preset number during shooting of the second working area. Therefore, when the preset number of target cells cannot be obtained in the shooting of the adjusted first working area or the second working area, the user can decide whether to enlarge the shooting area to the non-working area with poor effect.

A second aspect of the present invention provides a sample analysis system comprising:

the blood analysis device is used for detecting cells in a sample to be detected so as to obtain a cell detection result;

the sample image shooting device is used for shooting a sample to be detected on the sample carrier to be detected so as to obtain a cell image;

a controller communicatively connected with the blood analysis device and the sample image capture device, and configured to:

when the cell detection result does not include the low-value abnormal information of the target cells, controlling a sample image shooting device to shoot a first working area of the sample carrier to be detected so as to obtain a cell image containing a preset number of target cells;

and when the cell detection result comprises low-value abnormal information of the target cell, controlling the sample image shooting device to shoot a second working area of the sample carrier to be detected so as to obtain a cell image containing a preset number of target cells, wherein the second working area is larger than the first working area.

In the sample analysis system according to the second aspect of the present invention, before microscopic examination of a sample to be measured, it is first determined whether or not there is low-value abnormality information on target cells based on a cell detection result of a blood analysis apparatus, and the size of a working area is determined based on the determination result.

A third aspect of the present invention provides a sample image capturing method, including the steps of:

acquiring a first working area of a sample carrier to be detected carrying a sample to be detected and a preset number of target cells;

when the number of the target cells in the first working area is judged to be smaller than the preset number, adjusting the first working area to form a second working area; and

the second working area is image-captured so that a cell image containing a preset number of target cells can be obtained.

In some embodiments, the sample image capture method further comprises capturing the first working area to obtain a first cell image.

Preferably, it is determined whether the number of target cells in the first working area is less than a preset number based on the first cell image that has been captured.

In some embodiments, during the image capturing of the first working area, whether the capturing of the first working area is completed or not, when it is determined that the number of target cells within the first working area is less than the preset number, the first working area is adjusted in real time to form the second working area.

In some embodiments, the second working area may be determined according to the number of target cells that have been photographed and a difference between a preset number.

In some embodiments, it may be determined that the number of target cells in the first working area is less than the preset number according to the presence of low value abnormality of the target cells and/or bias of the first working area in the sample to be tested.

In some embodiments, adjusting the first working area to form the second working area may include expanding the first working area in a longitudinal direction and/or a transverse direction of the sample carrier to be measured on the basis of the first working area to form the second working area.

In some embodiments, the sample image capturing method further comprises:

when it is determined during the photographing of the second working area that the number of target cells in the second working area is less than the preset number, a prompt is output whether to perform photographing in the non-working area.

A fourth aspect of the present invention provides a computer-readable storage medium having stored thereon executable instructions that, when executed by a processor, cause the processor to implement the sample image capturing method described above.

Based on the aspects provided by the invention, when the number of the target cells in the first working area is predicted to be smaller than the preset number, the first working area is dynamically adjusted to form a second working area, and the sample image shooting device is controlled to shoot the second working area, so that a cell image containing a sufficient number of the target cells is obtained.

Other features of the present invention and advantages thereof will become apparent from the following detailed description of exemplary embodiments thereof, which proceeds with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:

fig. 1 is a schematic diagram of a sample analysis system according to some embodiments of the present invention.

Fig. 2 is a schematic structural diagram of an embodiment of the sample image capturing apparatus in fig. 1.

FIG. 3 is a schematic structural view of a smear of a sample to be tested according to some embodiments.

FIG. 4 is an image taken under a low power mirror in some embodiments.

FIG. 5 is a schematic view of the first working area of a smear of a sample to be tested.

FIG. 6 is a schematic view of a second working area of a smear of a sample to be tested.

Fig. 7 is a schematic structural diagram of a sample analysis system according to another embodiment of the present invention.

Fig. 8 is a flowchart illustrating a sample image capture method according to some embodiments of the invention.

Fig. 9 is a flowchart illustrating a sample image capturing method according to another embodiment of the invention.

Detailed Description

The embodiments of the invention will be described more fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that the terms "first \ second \ third" related to the embodiments of the present invention only distinguish similar objects, and do not represent a specific ordering for the objects, and it should be understood that "first \ second \ third" may exchange a specific order or sequence when allowed.

Referring to fig. 1, the present invention provides a sample analysis system 100 including a sample image capture device 110 and a controller 130.

The sample image capturing device 110 is configured to capture a test sample on a test sample carrier to acquire a cell image. The sample carrier to be tested can be a smear of a sample to be tested, for example a smear of blood to be tested.

Specifically, the sample image taking device 110 is configured to take an image of a sample component in a sample film smeared on a sample smear to be measured to obtain a sample component image. For example, when the sample is a blood sample, the sample smear is a blood smear to which a blood film is applied, and the sample component is particles, such as cells, in the blood sample at this time. At this time, the specimen image photographing device 110 is used to photograph cells in a blood film on a blood smear. Of course, the sample of the present invention may also be bone marrow or body fluid, etc.

In a specific example, as shown in fig. 2, the sample image photographing device 110 (e.g., a cell image photographing device) includes a micro optical module therein. The micro-optical module includes a lens group 111 and a camera 112, and the lens group 111 may include a first objective lens 1111 and a second objective lens 1112. The first objective lens 1111 may be a 10-fold objective lens or a 40-fold objective lens, for example, and the second objective lens 1112 may be a 40-fold objective lens or a 100-fold objective lens, for example. The lens group 111 may further include a switching mechanism 1113, and the switching mechanism 1113 is used to switch the first objective lens 1111 and the second objective lens 1112 so that the camera 112 captures images of different magnifications.

Further, as shown in fig. 2, the sample image taking device 110 further includes a sample carrier holding device, specifically in the present embodiment, the sample carrier holding device is a slide supporting device 113 for supporting the smear 21 of the sample to be measured, the slide supporting device 113 is arranged opposite to the micro optical module and is configured to be movable in three dimensions, so that the micro optical module can take an image of a specific area of the sample film of the smear 21 of the sample to be measured supported on the slide supporting device 113. The slide support device 113 is configured, for example, as a detection stage that can be moved in a horizontal plane, which detection stage can have a recess for receiving a smear 21 of a sample to be examined.

In some embodiments, the controller 130 is also electrically connected to the slide support 113 to control the relative movement of the slide support and the microscopy optical module.

An operation of the sample image taking apparatus 110 according to the present invention for taking a white blood cell in a blood smear as the smear 21 of the sample to be measured will be described with reference to fig. 3 to 5.

As shown in fig. 3 and 5, a blood film 211 is coated on the smear 21 of the sample to be measured, and a sample identification part 212 is provided on the blood film 211 side. The blood membrane 211 includes a head 2111, a body 2112 and a tail 2113, wherein the head 2111 is coated relatively thickly resulting in a dense cell distribution, while the tail 2113 is coated relatively thinly resulting in a sparse cell distribution, and thus is generally not suitable for white blood cell imaging in these two parts. The cells of the body portion 2112 are distributed relatively uniformly, and therefore a working area for white blood cells is generally searched for at the body portion 2112 or at the intersection of the body portion 2112 and the tail portion 2113.

When the sample image capturing device 110 captures white blood cells in a blood smear, a capturing area or a working area is first searched by the camera 112 under a low-power objective lens (e.g., a 10-power objective lens) of the first objective lens 1111 and the second objective lens 1112. In searching for the imaging area, the slide holder 113 and the micro-optics module are moved relative to each other so that the head 2111 of the blood smear can be moved under the macro objective to the tail 2113 thereof for searching, thereby determining the imaging area, which may be, but is not limited to, a single-layer cell area at the junction of the tail and the body of the blood smear (also referred to as the junction of the tail and the body).

After the determination of the recording area, the white blood cells located in the working area are scanned in a battlement-type manner by means of a camera and a low power objective. For example, the slide holder and the microscope optics module are moved relative to each other in such a way that the camera scans the working area on the blood smear under the macro objective lens in a column-wise or row-wise movement, in order to take a plurality of positions in the working area, and a predetermined number of white blood cells are located by analyzing the taken image, as shown in fig. 4. Here, the column-wise or row-wise moving scan may for example comprise moving the scan back and forth in the transverse or longitudinal direction of the blood smear.

After a preset number of white blood cells are identified and located, the located white blood cells are photographed by a camera under a high-power objective lens (e.g., 100-power objective lens) of the first objective lens 1111 and the second objective lens 1112. The slide supporting device 113 and the microscope optical module move relatively, so that the white blood cells positioned on the blood smear are sequentially positioned under the visual field of the high power objective lens and are shot by the camera.

As shown in fig. 1, in some embodiments, the sample analysis system 100 further includes a smear preparation apparatus 120. The smear preparing apparatus 120 prepares a smear of a sample to be measured using a biological sample to be measured by dropping at least one drop of the biological sample to be measured on a blank slide and then spreading a sample film or a sample coating on the blank slide to prepare the smear of the sample to be measured.

Further, as shown in fig. 1, the sample analysis system 100 further includes a first conveyance device 140 and a second conveyance device 150.

The first conveyor 140 includes a loading buffer 141, a feeding buffer 142, and an unloading buffer 143. When a sample to be tested in a test tube 11 on a test tube rack 10 needs to be subjected to a microscopic examination, the test tube rack 10 is transported to a smear preparing apparatus 120 to prepare a smear, the test tube rack 10 is first transported to a loading buffer 141, then transported from the loading buffer 141 to a feeding area 142 to prepare a smear by the smear preparing apparatus 120, and after the preparation of the smear is completed, unloaded from the feeding area 142 to an unloading buffer 143.

The smear preparing apparatus 120 receives the prepared smear in the slide basket 20, conveys the slide basket 20 receiving the smear 21 to be measured to the sample image photographing apparatus 110 by the second conveying apparatus 150, and photographs the cells in the sample on the smear to be measured and analyzes the photographed cell image by the sample image photographing apparatus 130.

In some embodiments, the sample analysis system 100 further includes a display device 160 for displaying the cell image and its analysis results. In the embodiment shown in FIG. 1, the display device 160 is provided separately from the sample image taking device 110 and the smear preparation device 120, and the display device 160 is communicatively connected to the sample image taking device 110. The sample image capturing device 110 transmits the captured cell image and the analysis result thereof to the display device 160 to be displayed. Of course, in other embodiments, the display device 160 may also be integrated into the sample image capture device 110 and communicatively connected to the micro-optics module and the controller of the sample image capture device 110.

In the embodiment shown in fig. 1, the controller 130 is configured to control the sample image photographing device 110 to photograph a sample to be measured.

The controller 130 may include, for example, a processor, RAM, ROM, a communications interface, memory, an I/O interface, and the like. The processing components, RAM, ROM, communications interface, memory, and I/O interface communicate over a bus. The processor may be a CPU, GPU or other chip with computing capabilities. The memory stores various computer programs to be executed by the processor, such as an operating system and an application program, and data necessary for executing the computer programs.

As shown in fig. 5, the controller 130 is configured to acquire a first working region M, which is a photographing region in which a size parameter (e.g., fixed length, fixed width, or fixed area) is set in advance, and then control the sample image photographing device 110 to photograph the first working region M. The size parameters of the first working area may be different for different samples. Specifically, as shown in fig. 3, the sample image taking device 110 takes a picture of the first working area M along a fixed serpentine path S (a battlement path) in order to, for example, identify and locate target cells within the first working area M. For example, the first working area M is divided into a plurality of rows uniformly in the longitudinal direction or the length direction a of the smear 21 of the sample to be measured, and then the plurality of rows are sequentially photographed in a serpentine path to obtain a cell image including a certain number of target cells.

However, clinically, counting values of white blood cells and red blood cells are required in microscopic examination, for example, wherein 100 white blood cells and at least 1000 red blood cells are required to be photographed, for example. For a sample to be detected with an abnormal condition, the image containing a sufficient number of target cells cannot be obtained by shooting the first working area which is preset. In view of this, an embodiment of the present invention provides a sample analysis system 100. The sample analysis system 100 includes a sample image capture device 110 and a controller 130. The structure of the sample image taking device 110 refers to the description of the above embodiment.

According to the present invention, the controller 130 is communicatively coupled to the sample image capture device 110. And is configured to: acquiring a first working area of a sample carrier to be detected carrying a sample to be detected and a preset number of target cells; when the number of the target cells in the first working area is judged to be smaller than the preset number, adjusting the first working area to form a second working area; and controls the sample image photographing device 110 to photograph the second working area so that a cell image containing a preset number of target cells can be obtained.

The first working area is a preset sample area or a sample area with a fixed area size set on the sample carrier to be measured, and the sample area is photographed by the sample image photographing device 110 to obtain a cell image which can be positioned to a preset number of target cells. The first working area can be obtained, for example, by referring to the above-described manner of operation of the sample image capture device with respect to leukocytes.

When the controller 130 determines that the number of target cells in the first working area is less than the preset number, i.e., the preset number of target cells cannot be obtained or located even though the photographing of the first working area is completed, the first working area is adjusted to form a second working area, and then the image photographing is performed on the second working area. Therefore, the sample image capturing method can, for example, expand a counting range or a working area for a sample to be measured in which an abnormal condition exists, thereby ensuring that a cell image including a sufficient number of target cells is obtained, for example, ensuring that a sufficient number of target cells are located. This is particularly advantageous in the case of leukocyte photography.

In some embodiments, the controller 130 is further configured to: the sample image capturing device 110 is controlled to capture the first working area to obtain a first cell image, and the number of the target cells in the first working area is determined to be smaller than the preset number according to the captured first cell image.

In some embodiments, the preset number of target cells may be obtained according to the cell image obtained before the first working region is adjusted and the cell image obtained after the first working region is adjusted, or the preset number of target cells may be obtained according to only the cell image obtained after the first working region is adjusted, which is not particularly limited by the present invention.

Taking the white blood cell count as an example, after the sample image capturing device confirms the first working area, the first working area is first captured by using the planned path, and the captured white blood cells are counted, and at the counting start stage, it is estimated whether the white blood cell count requirement can be completed by capturing the first working area (i.e., whether the preset number of white blood cells can be located) in real time by using the count value. Still adopting the first working area to shoot and count the normal samples; for abnormally low samples, the first working area or count range is expanded to ensure that the white blood cell count requirements can be fulfilled.

Specifically, during the control of the sample image capture device 110 to capture the image of the first working area, no matter whether the sample image capture device 110 finishes capturing the first working area, when it is determined that the number of target cells in the first working area is smaller than the preset number, the first working area is adjusted in real time to form the second working area. That is, the adjustment of the first working area is performed dynamically, for example, at the initial stage of image capturing of the first working area, it can be determined that a preset number of target cells cannot be obtained even if the first working area is completely captured according to the captured cell image, and then the first working area can be directly adjusted without waiting for the completion of image capturing of the first working area, thereby improving the working efficiency.

In some embodiments, the controller 130 may receive the first cell image captured by the sample image capturing device 110 and analyze the captured first cell image to determine whether to adjust the first working area. In other embodiments, the sample image capturing device 110 may directly analyze the first cell image captured by itself to determine whether to adjust the first working area, and then send the determination result to the controller 130, and the controller 130 determines whether to adjust the first working area directly according to the determination result.

In some embodiments, the controller is further configured to: and determining a second working area according to the number of the target cells obtained by shooting and the difference value between the preset numbers. For example, the first working area is dynamically enlarged to form the second working area according to the number of target cells that have been photographed. Here, the controller 130 predicts how large an area is needed again to photograph the preset number of target cells based on the difference between the number of target cells already photographed and the preset number, and then determines the range of the second working area based on the prediction.

In some embodiments, the controller is further configured to: and judging that the number of the target cells in the first working area is less than the preset number according to the low value abnormality of the target cells and/or the bias of the first working area in the sample to be detected. Specifically, the controller 130 may determine whether the target cell low value abnormality exists in the sample to be tested or whether the first working area offset exists according to the captured first cell image, and if the target cell low value abnormality exists or the first working area offset exists, determine that the number of the target cells in the first working area is smaller than the preset number. In one embodiment, when the controller 130 determines that the target cell low value abnormality exists in the sample to be tested according to the first cell image obtained by shooting, for example, the sample to be tested is a low value white cell sample or a low value red cell sample, the first working area is enlarged to form the second working area, or the working area is searched again. In another embodiment, when the controller 130 determines that the first working area offset exists in the sample to be tested according to the first cell image obtained by shooting, for example, when the controller 130 finds that the number of the target cells located at one side of the longitudinal center line of the smear 21 to be tested is significantly smaller than that at the other side when shooting along a column of the first working area, it can be determined that the sample film on the sample carrier to be tested has the offset, and the first working area can be expanded along the side of the first working area where the number of the target cells is large. Here, the first working area offset includes an asymmetrical, e.g. laterally offset, or inclined distribution of the sample film. Such an offset results in a reduction of the actual available counting area.

In other alternative embodiments, the controller 130 may also be configured to receive an appearance image of the sample film on the sample carrier to be tested, and determine whether the working area is biased directly according to the appearance image.

In some embodiments, the controller is further configured to: when the first working area is adjusted to form the second working area, the first working area is enlarged on the basis of the first working area along the longitudinal direction and/or the transverse direction of the sample carrier to be measured to form the second working area. Preferably, the length of the first working area and the second working area is the same, and when the first working area is enlarged, the enlargement is performed in the lateral direction of the first working area, that is, the width is enlarged. For example, referring to fig. 5 and 6, the first working area M is enlarged in the lateral direction of the sample carrier to be measured on the basis of the first working area M, and the first working area M is enlarged on both sides in the lateral direction of the sample carrier to be measured. For example, the first working area has a width of 12cm before adjustment and a width of 18cm after adjustment.

In some embodiments, the second working area comprises at least a partial area of the first working area. In the embodiment shown in fig. 5 and 6, the second operating region N comprises the first operating region M, that is to say the first operating region M is included in the range of the second operating region N. In other embodiments, the second working area comprises a partial area of the first working area, i.e. the second working area does not completely cover the first working area.

In other embodiments, the second working area is independent of the first working area. There is no portion of the second working area that overlaps the first working area. For example, the controller 130 determines that the sample to be tested is a low value red blood cell sample according to the cell image obtained by shooting, and when the blood membrane is found to have a problem that the counting value does not meet the clinical requirement due to vacuole, scratch, etc., for example, a suitable working area may be searched again for recounting, and the second working area and the first working area may be independent from each other. For example, the controller may determine that the sample thin film (e.g., blood film) is not uniform from the captured cell image, and the first working area is unusable, and may change the first working area to the second working area directly, or may change the capture area to the edge and/or tail of the sample thin film when platelet aggregation is found.

In some embodiments, the controller 130 is further configured to: and when the number of the target cells in the second working area is judged to be less than the preset number during shooting of the second working area, outputting a prompt of whether shooting is carried out in a non-working area. For example, for some low-value samples, after the first working area is adjusted and the cell image including the preset number of target cells is captured in the second working area, that is, after the counting range is expanded, a prompt may be output and the user may select whether to capture and count in the non-working area.

In other embodiments, the controller 130 may be further configured to: and acquiring a cell detection result of the blood analysis device on the sample to be detected, and judging whether the number of the target cells in the first working area is less than a preset number according to the cell detection result. For example, when the blood analysis device performs cell detection on the sample to be detected to obtain that the sample to be detected is a low-value white blood cell sample or a low-value red blood cell sample, the controller acquires the cell detection result from the blood analysis device, and then directly determines that the number of target cells in the first working area is smaller than the preset number according to the cell detection result.

Of course, in other embodiments, the controller may comprehensively determine whether the number of the target cells in the first working area is smaller than the preset number according to the cell detection result and the first cell image obtained by shooting, and then adjust the first working area according to the determination result.

Referring to fig. 7, the present invention also provides a sample analysis system 300 in another aspect, the sample analysis system 300 including a blood analysis device 310, a smear preparation device 320, a sample image photographing device 330, and a controller 340. The blood analysis device 310 is used for detecting cells in a sample to be detected to obtain a cell detection result. The sample image capturing device 330 is used for capturing a sample to be measured on the sample carrier to be measured.

The structure of the smear preparation apparatus 320, the sample image capture apparatus 330 and the controller 340 of the sample analysis system 300 can refer to the smear preparation apparatus 120, the sample image capture apparatus 110 and the controller 130 of the cell image analysis system 100 of the embodiment of FIG. 1, which are not described herein again.

The blood analysis device 310 is configured to detect particles in a biological sample to be tested to obtain particle detection results, such as blood routine detection results.

The cytological image analysis system 300 further comprises a first transport device 350 for transporting a rack 10, in which a plurality of test tubes 11 loaded with biological specimens to be tested can be placed, from the blood analysis device 310 to the smear preparation device 320, and a second transport device 360 for transporting a slide basket 20, in which a plurality of prepared smears 21 can be loaded, from the smear preparation device 320 to the sample image capture device 330. The controller 340 is electrically connected to the first conveyor 350 and the second conveyor 360 and controls the operation thereof. The second transporting device 360 further includes feeding mechanisms provided corresponding to the blood analyzing device 310 and the smear preparing device 320, respectively, each of which includes

loading buffer sections

311 and 321, feeding

detection sections

312 and 322, and unloading

buffer sections

313 and 323. The feeding mechanisms of the blood analysis apparatus 310 and the smear preparation apparatus 320 are connected by a transport rail.

When a biological sample to be tested on the test tube rack 10 needs to be transported to the blood analyzer 310 for testing, the test tube rack 10 is first transported from the transportation track to the loading buffer 311, then transported from the loading buffer 311 to the feeding detection area 312 for testing by the blood analyzer 310, and after the testing is finished, is unloaded from the feeding detection area 312 to the unloading buffer 313, and finally enters the transportation track from the unloading buffer 313.

Similarly, when the sample to be tested on the test tube rack 10 needs to be subjected to microscopic examination, the test tube rack 10 needs to be transported to the smear preparation device 320 for preparing the smear, the test tube rack 10 is first transported from the transport track to the loading buffer area 321, then transported from the loading buffer area 321 to the feeding detection area 322 for preparing the smear by the smear preparation device 320, and after the preparation of the smear is finished, the test tube rack is unloaded from the feeding detection area 322 to the unloading buffer area 323, and finally enters the transport track from the unloading buffer area 323. The smear preparing apparatus 320 receives the prepared smear in the slide basket 20, transports the slide basket 20 receiving the smear to be measured to the sample image photographing apparatus 330 by the second moving apparatus 360, and the sample image photographing apparatus 330 performs 3 photographing and analysis of cells in the sample on the smear to be measured.

The cytological image analysis system 300 further includes a display device 370 for displaying the results of the sample testing, which may be located on the blood analysis device 310, the smear preparation device 220, the sample image capture device 230, or otherwise.

The controller 340 is communicatively connected to the blood analysis device 310 and the sample image capture device 330, and is configured to:

when the cell detection result does not include the low-value abnormal information of the target cell, controlling the sample image shooting device 330 to shoot the first working area of the sample carrier to be detected so as to obtain a cell image containing a preset number of target cells;

and when the cell detection result comprises low-value abnormal information of the target cell, controlling the sample image shooting device 330 to shoot a second working area of the sample carrier to be detected so as to obtain a cell image containing a preset number of target cells, wherein the second working area is larger than the first working area.

For example, when the blood analysis apparatus 310 performs cell detection on a sample to be detected to obtain that the sample to be detected is a sample with a normal white blood cell or red blood cell count value, a first working area is selected for shooting; and if the sample to be detected is judged to be a low-value white blood cell sample or a low-value red blood cell sample, selecting a second working area larger than the first working area for shooting.

The invention also provides a sample image shooting method. As shown in fig. 8, the sample image photographing method includes the steps of:

s410, acquiring a first working area of a sample carrier to be detected carrying a sample to be detected and a preset number of target cells;

s430, when the number of the target cells in the first working area is judged to be smaller than the preset number, adjusting the first working area to form a second working area; and

s450, image-capturing the second working area so that a cell image including a preset number of target cells can be obtained.

When the controller 130 determines that the number of target cells in the first working area is less than the preset number, i.e., cell images of the preset number of target cells cannot be obtained even though the photographing of the first working area is completed, the first working area is adjusted to form a second working area, and then the image photographing is performed on the second working area. Therefore, the sample image shooting method can expand the counting range of the sample to be detected with abnormal conditions, and further obtain the cell image containing enough target cells.

As shown in fig. 9, in other embodiments, the sample image capturing method further includes step S420 of capturing the first working area to obtain a first cell image, and determining whether the number of target cells in the first working area is smaller than a preset number according to the captured first cell image.

Further, in the sample image capturing method, during image capturing of the first working area, no matter whether capturing of the first working area is completed, when it is determined that the number of target cells in the first working area is smaller than the preset number, the first working area is adjusted in real time to form the second working area.

Preferably, the second working area may be determined according to the number of target cells that have been photographed and a difference between a preset number.

In addition, the sample image photographing method may further include: when it is determined during the photographing of the second working region that the number of target cells in the second working region is less than the preset number, a prompt is output whether to photograph in the non-working region.

For other embodiments of the sample image capturing method provided by the present invention, reference may be made to the sample analysis system, which is not described herein again.

The present invention also provides a computer-readable storage medium having stored thereon executable instructions that, when executed by a processor, cause the processor to implement the sample image capturing method of the above-described embodiments.

The computer readable storage medium may be either volatile memory or nonvolatile memory, and may include both volatile and nonvolatile memory. The nonvolatile memory can be a read-only memory, a programmable read-only memory, an erasable programmable read-only memory, an electrically erasable programmable read-only memory, a magnetic random access memory, a flash memory, a magnetic surface memory, an optical disc, or a read-only optical disc; the magnetic surface storage may be disk storage or tape storage. Volatile memory may be random access memory, which acts as external cache memory. By way of illustration and not limitation, many forms of RAM are available, such as SRAM, SDRAM, DRAM, SDRAM, DDR SDRAM, SSRAM, SDRAM, and DMA bus RAM.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications of the embodiments of the invention or equivalent substitutions for parts of the technical features are possible; without departing from the spirit of the invention, it is intended to cover all modifications within the scope of the invention as claimed.

Claims

1. A sample analysis system, comprising:

a controller communicatively coupled to the sample image capture device and configured to:

obtaining a first working area of the sample carrier to be detected and a preset number of target cells,

and when the number of the target cells in the first working area is judged to be smaller than the preset number, adjusting the first working area to form a second working area, and controlling the sample image shooting device to shoot the second working area so as to obtain a cell image containing the preset number of the target cells.

2. The sample analysis system of claim 1, wherein the controller is further configured to: controlling the sample image shooting device to shoot the first working area so as to obtain a first cell image; and judging whether the number of the target cells in the first working area is less than the preset number or not according to the shot first cell image.

3. The sample analysis system of claim 2, wherein the controller is further configured to: when the number of the target cells in the first working area is judged to be smaller than the preset number, the first working area is adjusted in real time to form the second working area, no matter whether the sample image shooting device finishes shooting the first working area or not.

4. The sample analysis system of claim 2 or 3, wherein the controller is further configured to: and determining the second working area according to the number of the target cells obtained by shooting and the difference value between the preset numbers.

5. The sample analysis system of claim 1, wherein the controller is further configured to: and acquiring a cell detection result of the blood analysis device on the sample to be detected, and judging whether the number of the target cells in the first working area is less than the preset number according to the cell detection result.

6. The sample analysis system of claim 1, wherein the controller is further configured to: controlling the sample image shooting device to shoot the first working area so as to obtain a first cell image; obtaining a cell detection result of the blood analysis device on the sample to be detected; and judging whether the number of the target cells in the first working area is less than the preset number or not according to the cell detection result and the shot first cell image.

7. The sample analysis system of any of claims 1-6, wherein the controller is further configured to: and judging that the number of the target cells in the first working area is less than the preset number according to the low value abnormality of the target cells in the sample to be detected and/or the bias of the first working area.

8. The sample analysis system of any one of claims 1 to 7, wherein the second working area comprises at least a partial region of the first working area; or the second working area and the first working area are mutually independent.

9. The sample analysis system of any one of claims 1-7, wherein the controller is further configured to: when the first working area is adjusted to form the second working area, the first working area is expanded along the longitudinal direction and/or the transverse direction of the sample carrier to be detected on the basis of the first working area to form the second working area.

10. The sample analysis system of any one of claims 1 to 9, wherein the target cells are white blood cells or red blood cells.

11. The sample analysis system of any of claims 1-10, wherein the controller is further configured to: and when the number of the target cells in the second working area is judged to be less than the preset number during the shooting of the second working area by the sample image shooting device, outputting a prompt whether to shoot in a non-working area.

12. A sample analysis system, comprising:

the sample image shooting device is used for shooting the sample to be detected on the sample carrier to be detected so as to obtain a cell image;

a controller communicatively connected with the blood analysis device and the sample image capture device and configured to:

when the cell detection result does not include low-value abnormal information of the target cell, controlling the sample image shooting device to shoot a first working area of the sample carrier to be detected so as to obtain a cell image containing a preset number of the target cell;

and when the cell detection result comprises low-value abnormal information of the target cell, controlling the sample image shooting device to shoot a second working area of the sample carrier to be detected so as to obtain a cell image containing the preset number of the target cell, wherein the second working area is larger than the first working area.

13. A sample image shooting method is characterized by comprising the following steps:

image capture is performed on the second working area so that a cell image containing the preset number of target cells can be obtained.

14. The specimen image capturing method according to claim 13, further comprising capturing the first working area to obtain a first cell image; and judging whether the number of the target cells in the first working area is less than the preset number or not according to the shot first cell image.

15. The specimen image capturing method according to claim 13 or 14, wherein, during image capturing of the first working area, regardless of whether capturing of the first working area is completed, when it is determined that the number of target cells in the first working area is less than the preset number, the first working area is adjusted in real time to form the second working area.

16. The specimen image capturing method according to any one of claims 13 to 15, characterized in that the second working area is determined based on a difference between the number of target cells that have been captured and the preset number.

17. The method according to any one of claims 13 to 15, wherein it is determined that the number of target cells in the first working area is less than the preset number according to the presence of low-value abnormality of target cells and/or the offset of the first working area in the sample to be tested.

18. The sample image capturing method according to any one of claims 13 to 17, wherein the adjusting the first working area to form the second working area includes expanding the first working area in a longitudinal direction and/or a transverse direction of the sample carrier to be measured on the basis of the first working area to form the second working area.

19. The sample image capturing method according to any one of claims 13 to 18, characterized by further comprising:

outputting a prompt whether to perform photographing in a non-working area when it is determined that the number of the target cells in the second working area is less than the preset number during photographing of the second working area.

20. A computer readable storage medium having stored thereon executable instructions which, when executed by a processor, cause the processor to implement a sample image capture method according to any one of claims 13 to 19.