CN115389401A - On-line micro-channel square-hole flow chamber for microbial detection - Google Patents
On-line micro-channel square-hole flow chamber for microbial detection Download PDFInfo
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- CN115389401A CN115389401A CN202210920921.4A CN202210920921A CN115389401A CN 115389401 A CN115389401 A CN 115389401A CN 202210920921 A CN202210920921 A CN 202210920921A CN 115389401 A CN115389401 A CN 115389401A
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- 238000001514 detection method Methods 0.000 title claims abstract description 69
- 230000000813 microbial effect Effects 0.000 title description 2
- 239000007788 liquid Substances 0.000 claims abstract description 66
- 244000005700 microbiome Species 0.000 claims abstract description 18
- 239000010453 quartz Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000013459 approach Methods 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 description 14
- 239000002245 particle Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000003749 cleanliness Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000005304 optical glass Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- Dispersion Chemistry (AREA)
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Abstract
The invention discloses a square-hole flow chamber of a micro-channel for online microorganism detection. The liquid flow can be pressurized to the second-stage flow channel branch through the first pole flow channel branch, and the pressure when the second pole flow channel branches of the left flow channel and the right flow channel are converged is increased, so that the second pole flow channel branches of the left flow channel and the right flow channel are focused and converged to the liquid flow on two sides of the detection flow channel to increase the speed, more liquid flows on two sides are equal to or approach to the liquid flow flowing out of the middle flow channel, the width of the liquid flow with the constant center speed in the formed laminar state is widened, and the flow of the liquid flow flowing through the light spots is increased. The liquid flow width of the center of the detection flow channel is wide, the flow speed is high, the liquid in the flowing chamber can be always kept at a higher flow speed in a laminar flow state under the condition of not using the sheath liquid, and the sheath liquid can be completely avoided.
Description
Technical Field
The invention relates to a micro-channel square-hole flow chamber for online microorganism detection.
Background
In recent years, the development of microfluidics and flow cytometry is rapid and mature, and the microfluidics and flow cytometry are hot spots for the development of current micro total analysis systems. The traditional flow chamber is composed of a sample tube, a sheath fluid tank, a nozzle and the like, is made of transparent materials such as optical glass, quartz and the like, is fine in design and manufacture, and is the heart of a fluid flow system. The conventional sheath fluid forms a nuclear flow in the center of the fluid, so that the sample is limited on the axis of the fluid flow, but the detection flux of the detected sample is small, and the signal fluctuates under the influence of external oscillation.
In the existing flow type focusing scheme, the flow of the sample flow velocity which is ul/min generally cannot meet the real-time monitoring of a large flux (mL/min) of the online microorganism, in order to increase the flow velocity, a large amount of sheath fluid is wasted, and meanwhile, the cleanliness of the focusing sheath fluid is required to be far higher than that of a sample to be detected, so that the detection cost is improved. In addition, a very small amount of microorganisms carried by the sheath fluid can interfere with the detection of the microorganisms of the detected sample to a certain degree, the cleanliness of each batch of the sheath fluid has certain difference, the microorganism content of the sheath fluid needs to be calibrated before operation, the operation is complicated, and the algorithm identification and compensation difficulty is increased.
Disclosure of Invention
The invention provides an on-line microorganism detection micro-channel square-hole flow chamber which can avoid using sheath fluid and realize on-line microorganism detection, aiming at solving the problems existing in the prior art in use.
The technical scheme for solving the existing problems is as follows: the utility model provides an online little biological detection microchannel square hole flow chamber, includes the flow chamber body, the flow chamber body on be equipped with liquid inlet, runner area and be less than liquid inlet's square hole and detect the runner, the detection runner be equipped with the runner export, liquid inlet and detection runner between the middle runner that the intercommunication has the square hole, be located the left runner of middle runner both sides rejectable detection runner's square hole, the right runner of square hole, middle runner concentric with the detection runner, left runner, right runner all include that runner area is greater than the second pole runner branch that first pole runner branch, runner area and middle runner equal of middle runner's square hole, the second pole runner branch of left runner, right runner focus on detecting the runner.
As a further improvement, the flow area of the first pole flow channel branches of the left flow channel and the right flow channel is 1-2 times of the flow area of the middle flow channel.
As a further improvement, the flow area of the first pole flow channel branch of the left flow channel and the right flow channel is 1.5 times of the flow area of the middle flow channel.
As a further improvement, the first polar flow channel branches of the left flow channel and the right flow channel are parallel to the middle flow channel, and the second polar flow channel branches of the left flow channel and the right flow channel are obliquely converged.
As a further improvement, the middle flow channel, the left flow channel and the right flow channel are focused and converged at the same position on the detection flow channel.
As a further improvement, the flow chamber body is made of quartz.
As a further improvement, the left runner and the right runner are symmetrically arranged on two sides of the middle runner.
As a further improvement, the flow passage area of the middle flow passage is smaller than that of the square hole detection flow passage.
Compared with the prior art, the liquid detection device is characterized in that a middle flow channel with a square hole is communicated between the liquid inlet and the detection flow channel, a left flow channel and a right flow channel are positioned on two sides of the middle flow channel and can be converged into the square hole of the detection flow channel, the left flow channel and the right flow channel respectively comprise a first polar flow channel branch with a flow channel area larger than the square hole of the middle flow channel, and a second polar flow channel branch with a flow channel area equal to that of the middle flow channel, and the flow channel areas of the first polar flow channel branches of the left flow channel and the right flow channel are larger than that of the middle flow channel, and the flow channel areas of the second polar flow channel branches of the left flow channel and the right flow channel are equal to that of the middle flow channel and are focused on the detection flow channel. The liquid flow can be pressurized in the second stage flow channel branch through the first pole flow channel branch to increase the second pole of the left and right flow channelsThe pressure when the flow channel branches converge enables the second pole flow channel branches of the left flow channel and the right flow channel to focus and converge into the liquid flows on two sides of the detection flow channel to increase the speed, more liquid flows on two sides are equal to or approach to the liquid flow flowing out from the middle flow channel, the width of the formed liquid flow with the constant center speed in a laminar flow state is widened, and the purpose of increasing the flow of the liquid flow flowing through the light spots is achieved. The flow channel has the beneficial effects that the liquid flow width of the center of the detection flow channel is constant, the flow speed is high, the liquid in the flow chamber can always maintain a higher flow speed in a laminar flow state under the condition of not using sheath liquid, and the use of the sheath liquid can be completely avoided. For example, the liquid inlet flow rate of the flow chamber body is 5 multiplied by 10 -7 mm 3 When the flow rate is less than or equal to 3.6m/s, the flow rate of the ultrapure water to be detected can reach 30ml/min, and the requirement of online microorganism detection can be completely met.
Drawings
Fig. 1 is a schematic structural view of the present invention.
FIG. 2 is a schematic bottom view of a flow cell of the present invention.
Fig. 3 isbase:Sub>A sectional view taken along linebase:Sub>A-base:Sub>A of fig. 1.
FIG. 4 is a graph of the distribution of liquid flow rates within the flow chamber of the present invention.
FIG. 5 is a simulated distribution plot of the flow rate of a liquid in a flow chamber of the present invention.
Detailed Description
Referring to fig. 1-5, an on-line micro-channel square-hole flow chamber for detecting microorganisms comprises a flow chamber body 1, wherein a liquid inlet 2 for feeding liquid and a square-hole detection flow channel 8 with a flow channel area smaller than that of the liquid inlet 2 are arranged on the flow chamber body 1. The structure and the flow channel area ratio of the liquid inlet 2 and the detection flow channel 8 can adopt the prior art, and particularly the detection requirement of the detection flow channel 8 can be met. The end of the detection flow channel 8 is set as a flow channel outlet of liquid, the liquid inlet 2 and the detection flow channel 8 are communicated with a middle flow channel 7 with a square hole, the two sides of the middle flow channel 7 can be converged into a left flow channel 5 with the square hole of the detection flow channel 8 and a right flow channel 6 with the square hole, the middle flow channel 7 and the detection flow channel 8 are concentric, and the left flow channel 5 and the right flow channel 6 respectively comprise a first polar flow channel branch 3 with a flow channel area larger than the square hole of the middle flow channel 7 and a second polar flow channel branch 4 with a flow channel area equal to that of the middle flow channel 7.
The square hole detection flow channel 8 adopts a square hole to ensure that the excitation incident light is not refracted when being irradiated on the liquid flow through the flow chamber. The central fluid width widened in the central constant-speed liquid flow width in the laminar flow state formed in the square hole detection flow channel 8 is consistent with the width of a light spot irradiated to the flow chamber by the exciting light at the same time, so that the peak width of the particles with the same particle size is kept stable, and the microorganisms with different particle sizes can be perfectly distinguished in the subsequent process.
During detection, detection liquid continuously enters the detection flow channel 8 from the liquid inlet 2 through the left flow channel, the right flow channel and the middle flow channel 7 as required, the flow channel area of the first pole flow channel branch 3 is larger than the flow channel areas of the second pole flow channel branch 4 and the middle flow channel 7, the first pole flow channel branch 3 can pressurize the second pole flow channel branch 4 to increase the flow velocity of the liquid flow, the pressurized and accelerated liquid flow is focused and converged into the detection flow channel 8 from the second pole flow channel branch 4, the flow velocity of the liquid on two sides in the detection flow channel 8 is increased, the flow velocity of the liquid on two sides is equal to or close to the flow velocity of the liquid flowing into the middle of the detection flow channel 8 from the middle flow channel 7, and therefore the width of the liquid flow with the constant speed in the detection flow channel 8 in a laminar flow state is widened, and the flow of the liquid flow flowing through light spots is increased.
The flow area of the first pole flow channel branch 3 of the left flow channel 5 and the right flow channel 6 is 1-2 times of the flow area of the middle flow channel 7.
In the present embodiment, the flow area of the first pole flow channel branch 3 of the left flow channel 5 and the right flow channel 6 is preferably 1.5 times the flow area of the middle flow channel 7. When the flow area of the first pole flow channel branch 3 of the left flow channel 5 and the right flow channel 6 is 1.5 times of the flow area of the middle flow channel 7, and when the liquid flows of the left flow channel 5 and the right flow channel 6 are converged, the flow speed of the liquid flows on two sides can be increased by the second pole flow channel branch 4 pressurized by the first pole flow channel branch 3, so that the flow speed of the liquid flows on two sides in the detection flow channel 8 is increased, more liquid flows on two sides are close to the flow speed of the liquid flow in the middle of the detection flow channel 8, the width of the liquid flow with the constant speed in the center in the detection flow channel 8 in a laminar flow state is widened, and the flow of the liquid flow flowing through light spots is increased.
Liquid flow rate measurement see FIGS. 4-5The test distribution chart was set such that the width of the detection flow path 8 was 0.7mm and the flow rate at the inlet 2 of the liquid inlet was 5X 10 -7 mm 3 And(s) in the presence of a catalyst. The width of the liquid flow in which the detection flow channel 8 satisfies the flow measurement flow rate occupies almost the entire width of the detection flow channel 8 having a width of 0.7 mm. The flow rate at the inlet 2 of the liquid inlet is 5 multiplied by 10 -7 mm 3 When the flow rate is in the range of 3.6m/s, the flow rate of the outlet of the flow channel of the detection flow channel 8 is about 3.6m/s, and the flow rate of the ultrapure water to be detected can reach 30ml/min, so that the requirement of online microorganism detection can be completely met.
As can be seen from the liquid flow velocity test distribution simulation diagrams of fig. 4-5, after the liquid flows of the liquid flow through the middle flow passage 7, the left flow passage 5, and the right flow passage 6 converge, the flow velocities of the liquid flow above the convergence port in the radial direction in the detection flow passage 8 are approximately equal, and the flow width of the constant-velocity fluid almost occupies the pipe diameter of the whole detection flow passage 8. In the actual experiment, standard microspheres with the diameter of 7 mu m are added into liquid, and the peak widths of the peaks on an oscilloscope are basically consistent. I.e. to achieve substantially equal velocities of the liquid in the tube in the axial direction. Calculation of the fluid state at this time: re = vd/γ, where v is the flow rate of the liquid in the flow chamber, d is the diameter of the flow chamber tubing 0.7mm, and γ is the kinematic viscosity of water at 20 ℃ 1.007 × 10 -6 ν/m 2 s -1 Re is calculated to be equal to 791 < 2300, and 2300 is the boundary of laminar flow and turbulent flow of water. The flow of water in the pipe is a state of laminar flow.
The first polar flow channel branches 3 of the left flow channel 5 and the right flow channel 6 are parallel to the middle flow channel 7, and the second polar flow channel branches 4 of the left flow channel 5 and the right flow channel 6 obliquely converge. The left runner and the right runner can be symmetrically arranged on two sides of the middle runner.
Preferably, the middle flow channel 7, the left flow channel 5 and the right flow channel 6 are focused and converged at the same position on the detection flow channel 8. This allows optimal pooling of the microchannels.
The flow passage area of the middle flow passage 7 is smaller than that of the square hole detection flow passage 8.
The flow chamber body 1 is made of quartz, so that the incidence rate of exciting light is ensured.
Claims (8)
1. The utility model provides an online little biological detection microchannel square hole flow chamber, includes the flow chamber body, the flow chamber body on be equipped with liquid inlet, the runner area is less than the square hole detection flow way of liquid inlet, the detection flow way be equipped with the runner export, its characterized in that: the liquid inlet and the detection runner are communicated with a middle runner with a square hole, a left runner and a right runner are positioned on two sides of the middle runner and can be converged into the square hole of the detection runner, the middle runner and the detection runner are concentric, the left runner and the right runner respectively comprise a first polar runner branch with a runner area larger than the square hole of the middle runner, and a second polar runner branch with a runner area equal to that of the middle runner, and the second polar runner branches of the left runner and the right runner are focused and converged on the detection runner.
2. The micro flow channel square-hole flow cell for on-line microorganism detection according to claim 1, wherein: the flow area of the first pole flow channel branches of the left flow channel and the right flow channel is 1-2 times of the flow area of the middle flow channel.
3. The on-line micro flow channel square-hole flow cell of claim 1 or 2, wherein: the flow area of the first pole flow channel branches of the left flow channel and the right flow channel is 1.5 times of the flow area of the middle flow channel.
4. The micro flow channel square hole flow cell for on-line microorganism detection according to claim 1, wherein: the first pole flow channel branches of the left flow channel and the right flow channel are parallel to the middle flow channel, and the second pole flow channel branches of the left flow channel and the right flow channel are obliquely converged.
5. The micro flow channel square-hole flow cell for on-line microorganism detection according to claim 1, wherein: the middle runner, the left runner and the right runner are focused and converged at the same position on the detection runner.
6. The micro flow channel square-hole flow cell for on-line microorganism detection according to claim 1, wherein: the flow chamber body is made of quartz.
7. The micro flow channel square-hole flow cell for on-line microorganism detection according to claim 1, wherein: the left runner and the right runner are symmetrically arranged on two sides of the middle runner.
8. The micro flow channel square-hole flow cell for on-line microorganism detection according to claim 1, wherein: the flow passage area of the middle flow passage is smaller than that of the square hole detection flow passage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210920921.4A CN115389401A (en) | 2022-08-02 | 2022-08-02 | On-line micro-channel square-hole flow chamber for microbial detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210920921.4A CN115389401A (en) | 2022-08-02 | 2022-08-02 | On-line micro-channel square-hole flow chamber for microbial detection |
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| CN115389401A true CN115389401A (en) | 2022-11-25 |
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|---|---|---|---|
| CN202210920921.4A Pending CN115389401A (en) | 2022-08-02 | 2022-08-02 | On-line micro-channel square-hole flow chamber for microbial detection |
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- 2022-08-02 CN CN202210920921.4A patent/CN115389401A/en active Pending
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