CN104634719A - Flow chamber of flow cytometer - Google Patents
Flow chamber of flow cytometer Download PDFInfo
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- CN104634719A CN104634719A CN201410833078.1A CN201410833078A CN104634719A CN 104634719 A CN104634719 A CN 104634719A CN 201410833078 A CN201410833078 A CN 201410833078A CN 104634719 A CN104634719 A CN 104634719A
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Abstract
The invention discloses a flow chamber of a flow cytometer, and relates to the field of flow cytometers. By adopting the flow chamber, the problems that when the sample feeding rate of a sample flow of an existing flow cytometer is increased, to-be-tested cells or micro-particles are deviated from the central position of an irradiation light spot to cause that the irradiation energy is non-uniform, signal changes are increased, and data quality is reduced, and thus the detection precision of the cytometer is reduced can be solved. The flow chamber comprises an inlet area, a converging area, an adjusting area, a detection area and an auxiliary area, wherein the inlet area is provided with a main sheath liquid inlet, a sample flow inlet and an air pressure adjusting opening, the adjusting area is provided with two adjusting area inlets and a comprehensive adjusting area, main sheath liquid enters the inlet area from the sheath liquid inlet, the sample flow enters the converging area from a sample flow pipe, and the main sheath liquid and the sample flow are converged into a steady laminar flow in the converging area by using hydrodynamic focusing characteristics and then sequentially enter the comprehensive adjusting area, the detection area and the auxiliary area. According to the flow chamber disclosed by the invention, relatively high detection precision can be achieved at both high and low sample flow sample feeding rates, particularly, the nuclear flow diameter obtained after the sample flow is converged can be reduced at a high sample flow sample feeding rate, and high precision can be achieved.
Description
Technical field
The present invention relates to low cytometric analysis field, be specifically related to a kind of flow chamber of flow cytometer.
Background technology
Flow cytometer can carry out multiparameter, quantitative test or sorting to the cell be under quick flow state or biological particle, be widely used in the fundamental researchs such as cell biology, immunology, physiology, molecular biology, be also used to the aspect such as clinical diagnosis, environment measuring of medical science simultaneously.Flow chamber is as one of the critical component of flow cytometer, and Main Function is can single-row layout, orderly by laser detection district successively by the cell in sample suspensions or other particulates.Flow chamber utilizes fluid dynamics focusing principle, sheath fluid and sample stream are converged in flow chamber, sample stream is made to form stable laminar flow, be commonly called as core stream, now, sheath fluid is enclosed in around sample stream, and sample stream forms stable streamlined flow liquid by laser detection district, for optical system detection analysis.For same flow chamber, when sample stream sample rate improves, core flow diameter becomes large, cell to be measured in core stream or particulate depart from the center irradiating hot spot, make irradiation energy uneven, cause signal intensity to increase and quality of data decline, thus cell instrument accuracy of detection is declined.Therefore, the corresponding low sample stream sample rate of flow cytometer high measurement accuracy, the corresponding high sample stream sample rate of low accuracy of detection, user wants to obtain high sample stream sample rate and improves detection efficiency, must to sacrifice accuracy of detection for cost.Visible, all can keep, compared with the flow chamber of high measurement accuracy, there is very practical value under developing a kind of low sample stream sample rate and high sample stream sample rate.
Summary of the invention
When sample stream sample rate in order to the flow chamber existence solving existing flow cytometer improves, cell to be measured or particulate depart from irradiation spot center position, make irradiation energy uneven, signal intensity is caused to increase and quality of data decline, thus the problem that cell instrument accuracy of detection is declined, the invention provides a kind of flow chamber of flow cytometer.
The technical scheme that the present invention adopts for technical solution problem is as follows:
The flow chamber of a kind of flow cytometer of the present invention, comprising:
Inside is the inlet region of cylinder type hollow structure, and upper end, described inlet region is provided with main sheath fluid entrance, sample inflow entrance and air pressure adjustment mouth, and described sample inflow entrance is for installing sample stream pipe;
Inner be conical hollow-core construction and the assembling area of touching with lower end, inlet region, each cross section, inside, described assembling area circular diameter reduces from top to bottom gradually, and maximum cross-section circular diameter equals inlet region inner section circular diameter;
The adjustment district be made up of the adjustment district entrance that structure adjusting district is identical with two inner structures, entrance heavy caliber end inside, described adjustment district is cylinder type hollow structure, small-caliber end inside is cylinder type hollow structure or square hollow-core construction, inside middle is conical hollow-core construction, inside middle each cross section circular diameter is reduced gradually by direction, export-oriented structure adjusting district, maximum cross-section circular diameter equals heavy caliber end inner section circular diameter, described structure adjusting district is touched by zone of transition and lower end, assembling area upper end, two ends, left and right adjust district's entrance small-caliber end respectively and touch with two,
Inside is rectangle pipeline or cylindrical pipe and the detection zone of touching with lower end, structure adjusting district, and described structure adjusting district is identical with the shape and size of detection zone inner section, and structure adjusting district total length is less than detection zone;
The auxiliary region be connected is glued together with lower end, detection zone in upper end, and lower end, described auxiliary region is provided with outlet.
Described inlet region adopts resin or quartz glass to make, and outer shape is rectangular parallelepiped, square or right cylinder, and it is fixing for installing that mechanical interface is left in outside.
Described assembling area adopts resin or quartz glass to make, and outer shape is rectangular parallelepiped, square, right cylinder or cone.
Described assembling area inner section circle minimum diameter is 60 ~ 300 microns.
The one-body molded processing of laser is all adopted between described structure adjusting district and detection zone and between described inlet region and assembling area.
Described detection zone adopts quartz glass to make, and outer shape is rectangular parallelepiped or right cylinder.
Described auxiliary region outer shape is rectangular parallelepiped, square or right cylinder; Inside, lower end, described auxiliary region is cylinder type hollow structure, and inside, upper end is conical hollow-core construction, and inner each cross section, upper end circular diameter increases from top to bottom gradually, and maximum cross-section circular diameter equals lower end inner section circular diameter.
Described sample inflow entrance is between main sheath fluid entrance and air pressure adjustment mouth.
The outer shape of two adjustment district entrances is rectangular parallelepiped, square, right cylinder or cone.
When flow chamber is in low sample feeding speed mode of operation, two adjustment district entrances are all closed, when flow chamber is in high sample feeding speed mode of operation, the equal conducting of two adjustment district's entrances, main sheath fluid flows into inlet region from main sheath fluid entrance, sample stream pipe enters into inlet region along sample inflow entrance and arrives assembling area, sample stream in sample stream pipe flows into assembling area, main sheath fluid and sample stream are converged by fluid dynamics focusing principle and form stably stratified flow in assembling area, auxiliary sheath fluid enters into structure adjusting district respectively from two adjustment district entrances, keep main sheath flow quantity and sample stream flow constant, the flow of the auxiliary sheath fluid of adjustment, it is made to compress the stably stratified flow flowed into from assembling area, stably stratified flow is kept after sample stream is compressed, stably stratified flow flows into structure adjusting district and detection zone successively, flow out from auxiliary region after detection, the flow of main sheath fluid is more than or equal to the flow of auxiliary sheath fluid, the flow of auxiliary sheath fluid is greater than the sample rate of sample liquid.
The invention has the beneficial effects as follows: the flow chamber of a kind of flow cytometer of the present invention can realize flow cytometer and still can reach higher accuracy of detection under high sample stream sample rate and low sample feeding rate conditions, both ensure that the high measurement accuracy of low sample feeding speed, under achieving again high sample feeding speed, reduction sample flows through the stably stratified flow diameter after convergence, improves accuracy of detection.
Accompanying drawing explanation
Fig. 1 is the structural representation of the flow chamber of a kind of flow cytometer of the present invention.
In figure: 1, inlet region, 2, assembling area, 3, adjustment district, 4, detection zone, 5, auxiliary region, 6, main sheath fluid entrance, 7, sample inflow entrance, 8, air pressure adjustment mouth, 9, the first adjustment district entrance, the 10, second adjustment district entrance, 11, structure adjusting district, 12, sample stream pipe.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
As shown in Figure 1, the flow chamber of a kind of flow cytometer of the present invention is made up of inlet region 1, assembling area 2, adjustment district 3, detection zone 4 and auxiliary region 5, and compact siro spinning technology is integral successively for inlet region 1, assembling area 2, adjustment district 3, detection zone 4 and auxiliary region 5.Upper end, inlet region 1 is provided with main sheath fluid entrance 6, sample inflow entrance 7 and air pressure adjustment mouth 8, sample inflow entrance 7 is between main sheath fluid entrance 6 and air pressure adjustment mouth 8, sample inflow entrance 7 is for placing sample stream pipe 12, sample stream pipe 12 enters into inlet region 1 along sample inflow entrance 7 and arrives assembling area 2, air pressure adjustment mouth 8 detects and regulating device for connecting air pressure, the change of adjustment flow chamber internal gas pressure.
Inside, inlet region 1 is for having the cylinder type hollow structure of certain length, and inlet region 1 inner section is circular, and inner each cross section, inlet region 1 diameter of a circle is identical; Inlet region 1 outer shape is rectangular parallelepiped, square or right cylinder etc., and it is fixing for installing that mechanical interface is left in outside, inlet region 1.Adopt the one-body molded processing of laser between inlet region 1 and assembling area 2, lower end, inlet region 1 and upper end, assembling area 2 are touched, and the material of inlet region 1 and assembling area 2 is resin or quartz glass.
Inside, assembling area 2 is for having the conical hollow-core construction of certain length, assembling area 2 inner section is circular, inner each cross section, assembling area 2 circular diameter reduces from top to bottom gradually, assembling area 2 inner section circle maximum gauge is identical with inlet region 1 inner section circular diameter, assembling area 2 inner section circle minimum diameter is 60 ~ 300 microns, and inner section circular diameter concrete size in assembling area 2 determines according to the size of cell to be measured or particulate; Assembling area 2 outer shape is rectangular parallelepiped, square, right cylinder or cone etc., and assembling area 2 outer shape is preferably identical with inlet region 1 outer shape, is conducive to the one-body molded processing of laser, also can be different, designs as required.
Adjustment district 3 is provided with the first adjustment district entrance 9, second and adjusts district's entrance 10 and structure adjusting district 11 3 part, first adjustment district entrance 9 and the second adjustment district entrance 10 outer shape are rectangular parallelepiped, square, right cylinder or cone etc., first adjustment district entrance 9 can be identical with the second adjustment district entrance 10 outer shape, also can be different; It is identical that first adjustment district entrance 9 inner structure and second adjusts district's entrance 10 inner structure, first adjustment entrance 9 front end, district is heavy caliber end, first adjustment entrance 9 front end, district is inner for having the cylinder type hollow structure of certain length, front end inner section is circular, and inner each cross section, front end diameter of a circle is identical; First adjustment district entrance 9 inside middle is the conical hollow-core construction with certain length, inside middle cross section is circular, inside middle each cross section circular diameter is reduced gradually by direction, export-oriented structure adjusting district 11, and it is identical that inside middle cross section circle maximum gauge and first adjusts district's entrance 9 front end inner section circular diameter; First adjustment entrance 9 rear end, district is small-caliber end, and the first adjustment entrance 9 rear end, district is inner for having the cylinder type hollow structure of certain length or square hollow-core construction; Upper end, structure adjusting district 11 is touched by zone of transition and lower end, assembling area 2, structure adjusting district 11 left end and first adjusts district's entrance 9 small-caliber end and touches, structure adjusting district 11 right-hand member and second adjusts district's entrance 10 small-caliber end and touches, and adopts the one-body molded processing of laser between lower end, structure adjusting district 11 and detection zone 4.
Detection zone 4 outer shape is rectangular parallelepiped or right cylinder etc., the material of detection zone 4 is quartz glass, inside, detection zone 4 is for having rectangle pipeline or the cylindrical pipe of certain length, detection zone 4 inner section is circular or rectangle, structure adjusting district 11 inner cross-sectional shape, size and detection zone 4 inner cross-sectional shape, measure-alike, structure adjusting district 11 overall length is less than detection zone 4, when detection zone 4 and inside, structure adjusting district 11 are the rectangle pipeline with certain length, the inner section in detection zone 4 and structure adjusting district 11 is rectangle, the length x width size of structure adjusting district 11 inner section equals the length x width size of detection zone 4 inner section, when detection zone 4 and inside, structure adjusting district 11 are the cylindrical pipe with certain length, the inner section in detection zone 4 and structure adjusting district 11 is circle, structure adjusting district 11 inner section diameter equals detection zone 4 inner section diameter.
Auxiliary region 5 outer shape is rectangular parallelepiped, square or right cylinder etc., and upper end, auxiliary region 5 is small-caliber end, and upper end, auxiliary region 5 is inner for having the conical hollow-core construction of certain length, and upper end, auxiliary region 5 inner section is circular; Lower end, auxiliary region 5 is inner for having the cylinder type hollow structure of certain length, and lower end, auxiliary region 5 inner section is circular, and inner each cross section, lower end, auxiliary region 5 diameter of a circle is identical; Inner each cross section, upper end, auxiliary region 5 circular diameter increases from top to bottom gradually, and upper end, auxiliary region 5 inner section circle maximum gauge is identical with lower end, auxiliary region 5 inner section circular diameter; Small-caliber end and the lower end, detection zone 4 of upper end, auxiliary region 5 are glued together, and lower end, auxiliary region 5 is provided with outlet.
Main sheath fluid flows into inlet region 1 from main sheath fluid entrance 6, sample stream pipe 12 enters into inlet region 1 along sample inflow entrance 7 and arrives assembling area 2, sample stream in sample stream pipe 12 flows into assembling area 2, main sheath fluid and sample stream are converged by fluid dynamics focusing principle and form stably stratified flow in assembling area 2, and then stably stratified flow flow into structure adjusting district 11, detection zone 4 and auxiliary region 5 successively.
The flow chamber of a kind of flow cytometer of the present invention at least has two kinds of mode of operations.Exemplify two kinds of mode of operations in present embodiment, be respectively local-speed mode of operation and high-speed operation mode.When being in low sample feeding speed mode of operation, the first adjustment district entrance 9 and the second adjustment district entrance 10 are closed; When being in high sample feeding speed mode of operation, first adjustment district entrance 9 and second adjustment district's entrance 10 conducting, auxiliary sheath fluid enters into structure adjusting district 11 respectively from the first adjustment district entrance 9 and the second adjustment district entrance 10, keep main sheath flow quantity and sample stream flow constant, the flow of the auxiliary sheath fluid of adjustment, it is made to compress the stably stratified flow flowed into from assembling area 2, stably stratified flow is kept after sample stream is compressed, but diameter diminishes, cell or particle movement speed faster, enter detection zone 4 to detect, flow out from auxiliary region 5 after detection.The flow of auxiliary sheath fluid is greater than the sample rate of sample stream, and the flow of main sheath fluid is more than or equal to the flow of auxiliary sheath fluid.
Through said process, both ensure that the high measurement accuracy of low sample stream sample rate, under achieving again high sample stream sample rate, reduction sample flows through the stably stratified flow diameter after convergence, improves accuracy of detection.
Claims (10)
1. a flow chamber for flow cytometer, is characterized in that, comprising:
Inside is the inlet region (1) of cylinder type hollow structure, described inlet region (1) upper end is provided with main sheath fluid entrance (6), sample inflow entrance (7) and air pressure adjustment mouth (8), and described sample inflow entrance (7) is for installing sample stream pipe (12);
Inner be conical hollow-core construction and the assembling area (2) of touching with inlet region (1) lower end, described assembling area (2) inner each cross section circular diameter reduces from top to bottom gradually, and maximum cross-section circular diameter equals inlet region (1) inner section circular diameter;
The adjustment district (3) be made up of the adjustment district entrance that structure adjusting district (11) are identical with two inner structures, entrance heavy caliber end inside, described adjustment district is cylinder type hollow structure, small-caliber end inside is cylinder type hollow structure or square hollow-core construction, inside middle is conical hollow-core construction, inside middle each cross section circular diameter is reduced gradually by export-oriented structure adjusting district (11) direction, maximum cross-section circular diameter equals heavy caliber end inner section circular diameter, described structure adjusting district (11) upper end is touched by zone of transition and assembling area (2) lower end, two ends, left and right adjust district's entrance small-caliber end respectively and touch with two,
Inside is rectangle pipeline or cylindrical pipe and the detection zone (4) of touching with structure adjusting district (11) lower end, described structure adjusting district (11) is identical with the shape and size of detection zone (4) inner section, and structure adjusting district (11) total length is less than detection zone (4);
The auxiliary region (5) be connected is glued together in upper end and detection zone (4) lower end, and described auxiliary region (5) lower end is provided with outlet.
2. the flow chamber of a kind of flow cytometer according to claim 1, it is characterized in that, described inlet region (1) adopts resin or quartz glass to make, and outer shape is rectangular parallelepiped, square or right cylinder, and it is fixing for installing that mechanical interface is left in outside.
3. the flow chamber of a kind of flow cytometer according to claim 1, is characterized in that, described assembling area (2) adopt resin or quartz glass to make, and outer shape is rectangular parallelepiped, square, right cylinder or cone.
4. the flow chamber of a kind of flow cytometer according to claim 1 or 3, is characterized in that, described assembling area (2) inner section circle minimum diameter is 60 ~ 300 microns.
5. the flow chamber of a kind of flow cytometer according to claim 1, it is characterized in that, between described structure adjusting district (11) and detection zone (4) and between described inlet region (1) and assembling area (2), all adopt the one-body molded processing of laser.
6. the flow chamber of a kind of flow cytometer according to claim 1 or 5, is characterized in that, described detection zone (4) adopt quartz glass to make, and outer shape is rectangular parallelepiped or right cylinder.
7. the flow chamber of a kind of flow cytometer according to claim 1, is characterized in that, described auxiliary region (5) outer shape is rectangular parallelepiped, square or right cylinder; Inside, described auxiliary region (5) lower end is cylinder type hollow structure, and inside, upper end is conical hollow-core construction, and inner each cross section, upper end circular diameter increases from top to bottom gradually, and maximum cross-section circular diameter equals lower end inner section circular diameter.
8. the flow chamber of a kind of flow cytometer according to claim 1, is characterized in that, described sample inflow entrance (7) is positioned between main sheath fluid entrance (6) and air pressure adjustment mouth (8).
9. the flow chamber of a kind of flow cytometer according to claim 1, is characterized in that, the outer shape of two adjustment district entrances is rectangular parallelepiped, square, right cylinder or cone.
10. the flow chamber of a kind of flow cytometer according to claim 1, is characterized in that, when flow chamber is in low sample feeding speed mode of operation, two adjustment district entrances are all closed, when flow chamber is in high sample feeding speed mode of operation, the equal conducting of two adjustment district's entrances, main sheath fluid flows into inlet region (1) from main sheath fluid entrance (6), sample stream pipe (12) enters into inlet region (1) along sample inflow entrance (7) and arrives assembling area (2), sample stream in sample stream pipe (12) flows into assembling area (2), main sheath fluid and sample stream are converged by fluid dynamics focusing principle and form stably stratified flow in assembling area (2), auxiliary sheath fluid enters into structure adjusting district (11) respectively from two adjustment district entrances, keep main sheath flow quantity and sample stream flow constant, the flow of the auxiliary sheath fluid of adjustment, it is made to compress the stably stratified flow flowed into from assembling area (2), stably stratified flow is kept after sample stream is compressed, stably stratified flow flows into structure adjusting district (11) and detection zone (4) successively, flow out from auxiliary region (5) after detection, the flow of main sheath fluid is more than or equal to the flow of auxiliary sheath fluid, the flow of auxiliary sheath fluid is greater than the sample rate of sample liquid.
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| CN201410833078.1A CN104634719A (en) | 2014-12-29 | 2014-12-29 | Flow chamber of flow cytometer |
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| CN201410833078.1A CN104634719A (en) | 2014-12-29 | 2014-12-29 | Flow chamber of flow cytometer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106769809A (en) * | 2016-12-29 | 2017-05-31 | 重庆博奥新景医学科技有限公司 | A kind of flow cytometer and its 3 D video monitoring device |
| CN107907452A (en) * | 2017-11-03 | 2018-04-13 | 桂林优利特医疗电子有限公司 | Particle sheath stream imaging device |
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| CN106769809A (en) * | 2016-12-29 | 2017-05-31 | 重庆博奥新景医学科技有限公司 | A kind of flow cytometer and its 3 D video monitoring device |
| CN107907452A (en) * | 2017-11-03 | 2018-04-13 | 桂林优利特医疗电子有限公司 | Particle sheath stream imaging device |
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Application publication date: 20150520 |