CN106769809A - A kind of flow cytometer and its 3 D video monitoring device - Google Patents
A kind of flow cytometer and its 3 D video monitoring device Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N2015/1413—Hydrodynamic focussing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/144—Imaging characterised by its optical setup
- G01N2015/1445—Three-dimensional imaging, imaging in different image planes, e.g. under different angles or at different depths, e.g. by a relative motion of sample and detector, for instance by tomography
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of flow cytometer and its 3 D video monitoring device, the present invention relates to fluidic cell monitoring device technical field, the invention mainly comprises flow chamber, flow chamber includes capillary channel and the focusing chamber with inverted pyramidal structures, is imported at sample liquid and at least two to it and imports sheath fluid and focus on sheath fluid with sample liquid at capillary channel inlet by focusing on chamber inner wall, be wrapped to form columnar flow；A kind of flow chamber monitoring device, including the first light source of dual axis orthogonal, secondary light source, the first image collecting device and the second image collecting device, focal position, tight ness rating and long-time stability for monitoring sample stream in flow cytometer flow chamber.Present invention is mainly used for the core component of flow cytometer.
Description
Technical field
The present invention relates to fluidic cell monitoring device technical field, and in particular to a kind of flow cytometer and its 3 D video
Monitoring device.
Background technology
Flow cytometer (Flow Cytometer, abbreviation FCM) is present microscopy, chemiluminescence dyeing, electronics
Be able to grow up under the progressive combination of the synthesis in the field such as technology and computer for quick streamlined flow in it is slender
The characteristic and its composition of born of the same parents, or other various molecules (such as bacterium) and its loaded article carry out the height of multi parameter analysis and sorting
New instrument, the shape of its not only measurable cell size, internal particle also can detect cell surface and cytoplasmic antigen, cell
Interior DNA and rna content etc., can be analyzed to colony's cell on individual cell level.Business flow cytometer mainly includes
Optical system, electronic detection system and flow chamber.
Wherein flow chamber is the most crucial detection carrier of whole instrument, generally square or circular length and straight capillary
Pipeline.In the pipeline, all detected particles and its place sample liquid are intended in two dimensional fluid focusing force
In the presence of (hydrodynamic focusing), closely it is strapped in the middle of pipeline and forms the post that individual particle is passed sequentially through
The ultra-fine laminar flow of shape, its diameter of section or length (width) are generally at 10 μm or so.The particles such as cell, microballoon therefore with sample liquid together by
It is strapped in the columnar flow, and is excited in fixed position by laser (its spot size is usually 20 μm -80 μm), and then
Detected and recognized by scattering light and fluorescence detecting system.
By two dimensional fluid is focused on, formed cylindrical sample stream is very tiny, and conventional mechanical processing can be introduced with preparation technology
Certain error (>50 μm), focus on sample in the flow chamber that these errors cause different batches to produce, different personnel install out
There is consistent sex differernce in the position of stream, degree of stability, and uniform, clearly fluid distrbution cannot be presented in capillary channel, be
The assembling of instrument other systems proposes certain test with debugging process.Present image harvester is single shaft, and causes prison
Measurement information is using insufficient, and the technical problem such as recognition effect difference.
The content of the invention
For above-mentioned problem of the prior art, present invention aim at a kind of flow cytometer of offer and its 3 D video prison
Device is surveyed, prior art its flow chamber sample stream is solved and be there is unstable focus and cannot be presented in capillary channel uniformly, clearly
Clear fluid distrbution, its image collector is set to single shaft and cannot provide the technical problems such as the three-dimensional space position of columnar flow.
The invention provides the video frequency monitoring system of a set of dual axis orthogonal, feelings are focused on to sample stream from two spaces direction
Condition is shot, and by software automatic decision its relative position, width and long-time stability, be it is also proposed herein a set of corresponding
Flow chamber focusing effect test and adjustment method, capillary is stably focused on sample stream in reaching all flow chambers
The purpose in road axle center.
To reach above-mentioned purpose, the technical solution adopted by the present invention is as follows：
A kind of flow chamber for flow cytometer, including capillary channel and the focusing chamber with inverted pyramidal structures, gather
Burnt chamber tapering connection capillary channel, junction as sample liquid capillary channel inlet；The focusing chamber is also connected with
Sheath fluid, sheath fluid is focused on sample liquid at the capillary channel inlet by the inwall of the focusing chamber, is wrapped to form post
Shape stream.
In such scheme, the sidepiece or top planes for focusing on chamber is provided with sheath fluid introducing port and sample liquid is imported
Mouthful.
In such scheme, the sheath fluid introducing port, its quantity at least includes two；The sample liquid introducing port, is arranged on
Focus on the reverse taper axis of chamber, and the adjustable sample pin in a position is set and institute is imported by the sample liquid introducing port
State sample liquid.
A kind of monitoring device for flow cytometer, including
Capillary channel, is connected with a columnar flow comprising sample liquid；
First light source, irradiates capillary channel, and cause columnar flow scattering light；
First image collecting device, receives the rear orientation light or side scattered light of capillary channel cylindrical stream；
Second image collecting device, rear orientation light or side scattered light that the first image collecting device of correspondence is received,
Receive remaining side scattered light or rear orientation light.
In such scheme, the second described image collecting device, or receive forward scattering light.
In such scheme, also including secondary light source, with the first described light source, the first image collecting device and the second image
Harvester is flowing to scattering caused by the transmitting of correspondence light and scattering light in vertical plane relative to capillary channel cylindrical stream
Reception relation antarafacial ground is coplanar in cross shaped mounting structure or X letter shape mounting structures, cross shaped mounting structure or X letters
The center of shape mounting structure is on capillary channel axis.
In such scheme, the secondary light source is set by the reflection of the first light source, refraction or beam splitting and obtained, or selected
Complementary frequency spectrums have the light source of overlapping frequency spectrum.
A kind of flow cytometer component, including
Flow chamber, including capillary channel and the focusing chamber with inverted pyramidal structures, sample liquid and at least two are imported to it
Place imports sheath fluid and focuses on sheath fluid with sample liquid at capillary channel inlet by focusing on chamber inner wall, is wrapped to form column
Stream；
Monitoring device, including the first light source, secondary light source, the first image collecting device and the second image collecting device；
Described the first light source, secondary light source, the first image collecting device and the second image collecting device, relative to hair
Thin pipe cylindrical stream flows to the position of capillary channel scattering and scattering light-receiving caused by the transmitting of correspondence light in vertical plane
Relation antarafacial ground is coplanar in cross shaped mounting structure or X letter shape mounting structures, cross shaped mounting structure or X letter shape peaces
The center of assembling structure is on capillary channel axis.
In such scheme, described capillary channel selects high transparency, the material without autofluorescence, acid and alkali-resistance；The capillary
Pipeline is shaped as straight long pipeline, and its inner section is square or rectangle, and outer section correspondence inner section is square or long
It is square.
In such scheme, described the first image collecting device and the second image collecting device, including shoot camera and
The collection camera lens of camera matching is shot, camera assembling is shot and is collected camera lens and be respectively arranged in a three-dimensional trim holder；Described
First light source is aligned with the first image collecting device and constitutes first axle monitoring of structures, and described secondary light source is adopted with the second image
Acquisition means are aligned and constitute the second shaft monitoring structure, and the location mid-shaft relation of first axle monitoring of structures and the second shaft monitoring structure is
It is orthogonal.
Compared with prior art, beneficial effects of the present invention：
1. two sheath fluids are introduced directly into from chamber roof or side wall, are formed around sample pin and surround, and in outgoing
Two dimensional fluid is formed around sample stream and focuses on power, focused in the wall of chamber tapering, be rolled into its sample stream as scabbard
Columnar flow, cylindrical flow flat slides into capillary duct, and keeps its original focusing structure, realizes stabilization in knitting wool pipeline
Cell flow and the sheath stream-sample of clear, easy differentiation is formd on the longitudinal cross-section flowed to along knitting wool pipeline cylindrical stream
The fluid distrbution of product stream-sheath stream；
2. every group of light source and condenser, collect camera lens and shoot that camera is coaxial staggered relatively, what light source and condenser sent
Light source passes through capillary, illuminates its nucleus, and converges to shooting camera imaging face, two groups of illuminations and bat through camera lens is collected
Take the photograph system altitude to be in same level, and four orientation are occupied in 90 ° of orthogonal modes, form the double vision to capillary channel
Angle stereovision；
3. the present invention passes through Double-visual angle stereoscopic monitoring structure, moreover it is possible to realize that Detection results are compensated mutually, and 90 ° of quadrature positions are closed
The monitoring of structures of the system lateral scattering that can mutually be consistent just is received and required, i.e. the first light source is to the back scattering of capillary channel by the
One image collecting device is received and its lateral scattering will compensate the collection result of the second image collecting device, and secondary light source is to hair
The back scattering of thin pipe is received by the second image collecting device and its lateral scattering will compensate the first image collecting device
Collection result, dexterously realizes extra effect enhancing；
4. the present invention make minor adjustment for alignment position with can feeding back when mounted with ensure to realize bidirectional eccentric away from
Minimum, that is, the uniformity of each sample stream position, columned into one to focusing on institute in capillary channel after keeping flow chamber to install
Liquid fluidization tower width, the carrying out of relative position automatic detection, also flow chamber two dimensional fluid dynamic focus effect can be judged,
Monitoring and evaluation, improves the yields of production flow chamber.
Brief description of the drawings
Fig. 1 is flow cytometer schematic diagram of the invention, and 1 is the focusing chamber of flow chamber, and 2L, 2R are positioned at focusing chamber
The sheath fluid introducing port of side, 3 is sample liquid introducing port, and 4 is capillary channel；
Fig. 2 be (left side) flow chamber installation relation of the invention, (in) position relationship, (right side) of focusing sample stream and capillary channel
The schematic diagram of capillary channel cross section；
Fig. 3 is 3 D video monitoring of structures top view of the present invention；
Fig. 4 is 3 D video monitoring of software analysis system exemplary plot of the present invention, and (upper left) is the first image collecting device institute
The recognition result of image, (upper right) are the recognition result of the second image collecting device gained image, (lower-left) to focus on space
The recognition result of position, (bottom right) are each parameter evaluation；
Fig. 5 is video analysis data accounting example figure of the present invention；
Fig. 6 is that flow chamber installs allotment flow chart.
Specific embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine by any way.
The present invention will be further described below in conjunction with the accompanying drawings：
Embodiment
The invention provides a kind of 3 D video monitoring device for flow chamber assembling and its recognition methods, it is right to complete
Columned liquid fluidization tower width, an automatic detection for relative position are focused into capillary channel, the device is mainly used in streaming
Judgement, the monitoring and evaluation of the two dimensional fluid dynamic focus effect of cell instrument core component, i.e. flow chamber, and be position when it is installed
Minor adjustment provide reference frame.
Whole monitoring system includes two sets of light sources, focus lamp, collects camera lens, shoots camera and three-dimensional trim holder, wherein light
Source and condenser combination accessory are used, and for forming collimation hot spot of the diameter slightly larger than capillary channel external diameter, and illuminate its pipeline
Middle body；Collect camera lens and shoot camera and support the use, imaging note is carried out for the liquid stream situation in the pipeline to capillary
Record, internal diameter of its visual field slightly larger than capillary channel；Collect camera lens and shoot after camera tightens assembling and be arranged on three-dimensional trim holder,
For the locus adjustment of video detection.
Such as Fig. 3, every group of light source and condenser, collection camera lens and shooting camera are coaxial staggered relatively, and light source and condenser are sent out
The light source for going out passes through capillary, illuminates its nucleus, and converges to shooting camera imaging face through camera lens is collected.Two groups of illuminations
With camera system highly in same level, and four orientation are occupied in 90 ° of orthogonal modes, formed to capillary channel
Double-visual angle stereovision.Three-dimensional trim holder be used for implement illumination path and collect light path alignment, and provide adjust up and down from
By spending, for realizing the tracking monitor to capillary channel different height position.
Such as Fig. 2, core observation and detection object are capillary interior conduit and its fluid in the present invention, more specifically in capillary
A sample stream for thin columnar flow is focused into pipeline, and it is relative to the locus of capillary channel inwall.The capillary
Road is preferentially constituted by high transparencies such as glass, quartz, without materials such as autofluorescence, acid-fast alkali-proofs, and capillary channel is straight long pipeline,
Its inner section can be square or rectangle, and outer section can be corresponding square or rectangle.
As Fig. 1, the capillary channel entrance are installed in focusing chamber outlet, flow chamber is monolithically fabricated up and down.Focus on chamber
It is inverted pyramidal structures, except in addition to the outlet of the vertex of a cone, also having three entrances, including two sheath fluid entrances, one in top planes
Individual sample liquid entrance.Wherein sample liquid is imported by sample pin, and its needle point stretches out and extends to focusing chamber centre position, the sample pin
The axle center for focusing on chamber should be located at.Two sheath fluids are introduced directly into from chamber roof plane or side wall, are formed around sample pin
It surround, and two dimensional fluid is formed around the sample stream of outgoing and focuses on power.The outlet of chamber tapering is being focused on, will as scabbard
Its sample stream is rolled into columnar flow.Finally, these fluids are smooth enters capillary duct, and keeps its original focusing structure.
In the feature, sample columnar flow with respect to capillary channel wall position, depending on sample pin setting angle and
Position, the result of this video surveillance can provide foundation for its minor adjustment.
The video surveillance refers to the state that twin shaft camera photographs fluid in capillary duct, and by software identifying system
Corresponding informance is automatically identified, real-time quantitative provides sentence read result and the process for recording.For every suit imaging system, institute
Stating software identification includes：Whether image quality clear, capillary duct whether in video, the knowledge of capillary tube inner wall right boundary
Not, the identification of sample stream right boundary.Recorded by the identification of this four edges circle and position, can be calculated sample stream width,
Focus on the distance of center position capillary or so the inwall of liquid fluidization tower, sample flow center line and capillary tube inner wall center it is inclined
The heart away from etc. information.By orthogonal double video summaries, horizontal multi-parameter is obtained, the information of lateral multi-parameter draws focusing sample stream
Two-dimensional coordinate schema mapping on capillary channel cross section, as shown in Figure 4.For evaluate flow chamber working condition whether just
Often, and by adjusting the position of sample pin, bidirectional eccentric is obtained away from minimum purpose.
These data can also carry out the one-parameter statistics such as maximum, minimum value, average value, standard deviation, the coefficient of variation
Credit is analysed, it is also possible to the correlation analysis with lateral ratio before carrying out.By the video monitoring device, can also non-volatile recording
The parameter informations such as relative position, the width of flow focusing, carry out sheath stream stabilization time, change width amplitude, change in location amplitude
Etc. the evaluation of index, as shown in Figure 5.Estimation of stability is carried out to the streaming room that each is assembled, help improves the product that dispatches from the factory
Yields.
In shown feature, camera lens, including multiple convex lens are collected, and for will be moved in capillary tube inner wall edge and inwall
Fluid is enlarged into the real image for being adapted to shoot camera photosurface size in the picture for producing.The collection camera lens includes that first can be included
Convex lens, the second convex lens and the 3rd convex lens, the second convex lens are located between the first convex lens and the 3rd convex lens, and the convex lens can be quartz
Glass.
It is of the present invention, collect camera lens and camera adjustment frame possesses the three-dimensional adjustment free degree, first can respectively in level
Secondly face two-dimension adjustment can be also adjusted, to capillary in the height direction simultaneously with causing illumination and imaging moiety coaxial
Road different height detected, obtains the two-dimensional signal schema mapping at different height position.
Of the present invention, flow chamber can also be rotated around capillary axle center, poly- to sample stream in the case of different visual angles
The detection of burnt characteristic.Two groups of numerical value are recorded by Orthogonal Double detecting system under each angle, by 180 ° of rotations plus automatic identification
Describe, 360 ° of true focus situations of degree of sample stream can be obtained.
In specific embodiment, capillary can be using the square quartz glass of 200 μm of external diameter 8mm of internal diameter, video acquisition phase
Machine can be using 1/2 inch of 640*480 pixels of CCD camera, and collecting camera lens can use 17X telephoto lenses, overall space point
Resolution is 0.925 μm, and frequency acquisition is 60fps.
In a kind of embodiment, the assemble flow of flow chamber can be as follows：
S1：Pre-assembled is carried out to flow chamber part, and connects upper exterior line, with 0.1M sodium hydroxide solutions, cleaning fluid
Flow chamber twice is alternately cleaned, residual impurity is removed；
S2：Sheath fluid pipeline is passed through sheath fluid, and sample pipe is passed through calibration solution, in the case where sample introduction ratio is tested, forms column sheath
Stream.It is analyzed in this three-dimension monitor system；
S3：Analyzed by video software, obtain sample stream in forward direction and lateral eccentric throw；The shaft position of sample introduction needle two is carried out
Fine setting, until both direction eccentric throw is zero；
S4：Analysis sample stream, before obtaining to lateral width, and compared with standard value；Continuous monitoring sample stream
Width and eccentric throw information, obtain estimation of stability；
The calibration solution can be all kinds of extinctions high, colored solutions, including but not limited to methyl blue, methylene blue, ocean
The solution such as red, rhodamine, crystal violet.
In addition, it is necessary to illustrate, the parameter of light source, focus lamp, collection camera lens and camera is more flexible, those skilled in the art
Can be according to actual selected, the key parameter of non-invention.
The above, specific embodiment only of the invention, but protection scope of the present invention is not limited thereto, and it is any
Belong to those skilled in the art the invention discloses technical scope in, the change or replacement that can be readily occurred in, all should
It is included within the scope of the present invention.
Claims (10)
1. a kind of flow chamber for flow cytometer, it is characterised in that poly- including capillary channel and with inverted pyramidal structures
Burnt chamber, focuses on the tapering connection capillary channel of chamber；The focusing chamber is also connected with sheath fluid, and sheath fluid is by the focusing chamber
Inwall at the capillary channel inlet with sample liquid focus on, be wrapped to form columnar flow.
2. a kind of flow chamber for flow cytometer according to claim 1, it is characterised in that the focusing chamber
Sidepiece or top planes are provided with sheath fluid introducing port and sample liquid introducing port.
3. a kind of flow chamber for flow cytometer according to claim 2, it is characterised in that the sheath fluid is imported
Mouthful, its quantity at least includes two；The sample liquid introducing port, is arranged on the reverse taper axis for focusing on chamber, and set
The adjustable sample pin in one position imports the sample liquid by the sample liquid introducing port.
4. a kind of monitoring device for flow cytometer, it is characterised in that including
Capillary channel, is connected with a columnar flow comprising sample liquid；
First light source, irradiates capillary channel, and cause columnar flow scattering light；
First image collecting device, receives the rear orientation light or side scattered light of capillary channel cylindrical stream；
Second image collecting device, rear orientation light or side scattered light that the first image collecting device of correspondence is received, receives
Remaining side scattered light or rear orientation light.
5. a kind of monitoring device for flow cytometer according to claim 4, it is characterised in that the second described figure
As harvester, or receive forward scattering light.
6. a kind of monitoring device for flow cytometer according to claim 4 or 5, it is characterised in that also including
Two light sources, with the first described light source, the first image collecting device and the second image collecting device in relative to capillary channel
Columnar flow flows to corresponding light in vertical plane with launching caused scattering and scattering light-receiving relation antarafacial or coplanarly in ten
The center of font mounting structure or X letter shape mounting structures, cross shaped mounting structure or X letter shape mounting structures is in capillary channel
On axis.
7. a kind of monitoring device for flow cytometer according to claim 6, it is characterised in that second light
Source, is reflected, refraction or beam splitting set and obtain by the first light source, or from complementary frequency spectrums or has a light source of overlapping frequency spectrum.
8. a kind of flow cytometer component, it is characterised in that including
Flow chamber, including capillary channel and the focusing chamber with inverted pyramidal structures, import to it and are led at sample liquid and at least two
Enter sheath fluid and sheath fluid is focused at capillary channel inlet with sample liquid by focusing on chamber inner wall, is wrapped to form columnar flow；
Monitoring device, including the first light source, secondary light source, the first image collecting device and the second image collecting device；
Described the first light source, secondary light source, the first image collecting device and the second image collecting device, relative to capillary
Road cylindrical stream flows to the position relationship of capillary channel scattering and scattering light-receiving caused by the transmitting of correspondence light in vertical plane
Antarafacial ground is coplanar in cross shaped mounting structure or X letter shape mounting structures, and cross shaped mounting structure or X letter shapes install knot
The center of structure is on capillary channel axis.
9. a kind of flow cytometer component according to claim 8, it is characterised in that described capillary channel from it is high thoroughly
Material bright, without autofluorescence, acid and alkali-resistance；The capillary channel is shaped as straight long pipeline, its inner section for square or
Rectangle, outer section correspondence inner section is square or rectangle.
10. a kind of flow cytometer component according to claim 8 or claim 9, it is characterised in that the first described IMAQ
Device and the second image collecting device, including shooting camera and shooting the collection camera lens that camera is matched, shoot camera assembling and receive
Collection camera lens is simultaneously respectively arranged in a three-dimensional trim holder；The first described light source is aligned with the first image collecting device and constitutes
One shaft monitoring structure, described secondary light source is aligned with the second image collecting device and constitutes the second shaft monitoring structure, first axle
The location mid-shaft relation of monitoring of structures and the second shaft monitoring structure is orthogonal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611242525.1A CN106769809A (en) | 2016-12-29 | 2016-12-29 | A kind of flow cytometer and its 3 D video monitoring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611242525.1A CN106769809A (en) | 2016-12-29 | 2016-12-29 | A kind of flow cytometer and its 3 D video monitoring device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
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| CN107389535A (en) * | 2017-07-18 | 2017-11-24 | 深圳小孚医疗科技有限公司 | A kind of means of illumination of micro-imaging illuminator |
| CN110686144A (en) * | 2019-10-10 | 2020-01-14 | 浙江大学 | Dripping form image acquisition device in dripping process of dripping pills |
| CN110770571A (en) * | 2017-07-18 | 2020-02-07 | 贝克顿·迪金森公司 | Dynamic interactive display of multi-parameter quantitative biological data |
| CN114112872A (en) * | 2021-11-22 | 2022-03-01 | 常州必达科生物科技有限公司 | Device and method for calibrating sample flow position in flow type pool |
| CN116008157A (en) * | 2023-03-28 | 2023-04-25 | 赛雷纳（中国）医疗科技有限公司 | Optical path device and flow cytometer |
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