CN115389285A - Fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation method and device - Google Patents
Fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation method and device Download PDFInfo
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- CN115389285A CN115389285A CN202211007908.6A CN202211007908A CN115389285A CN 115389285 A CN115389285 A CN 115389285A CN 202211007908 A CN202211007908 A CN 202211007908A CN 115389285 A CN115389285 A CN 115389285A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/85—Investigating moving fluids or granular solids
Abstract
The invention discloses a fluorescent carbon quantum dot assisted magnetofluid dynamic characteristic image observation method and device, wherein the method comprises the following steps: s1: preparing fluorescent carbon quantum dots; s2: preparing magnetic fluid; s3: mixing the fluorescent carbon quantum dots and the magnetic fluid to enable the fluorescent carbon quantum dots to be wrapped on the magnetic fluid to form a magnetic fluid composite functional material; s4: and placing the magnetic fluid composite functional material in a magnetic field to obtain a dynamic characteristic image of the magnetic fluid composite functional material. The invention utilizes the fluorescence characteristic of the novel fluorescent nano material carbon quantum dot to observe the microstructure and the microstructure evolution rule of the magnetic fluid under the action of the magnetic field and reveal the change of the micro dynamics.
Description
Technical Field
The invention relates to the technical field of magnetofluid dynamic characteristic observation, in particular to a magnetofluid dynamic characteristic image observation method and device assisted by fluorescent carbon quantum dots.
Background
The magnetic fluid is also called magnetic fluid, ferrofluid or magnetic liquid, is a novel functional material invented in the 60's of the 20 th century in the United states, and mainly consists of magnetic nanoparticles with the diameter of nanometer level (below 10 nm), base carrier liquid and surfactant. Due to the action of the surfactant, the magnetic fluid has enough stability, and agglomeration and sedimentation can not occur under the action of a gravity field, an electric field and a magnetic field. The colloidal solution can flow like liquid and can be attracted by a magnetic field like a solid magnetic material. The magnetic fluid does not exhibit magnetism at ordinary times, and can exhibit magnetism when a magnetic field is applied thereto, that is, the magnetic fluid can perform a certain movement.
Researches show that under the action of a magnetic field, the macroscopic mechanical property of the magnetic fluid is controlled by the microstructure characteristics of the magnetic fluid, and the dynamic change can be more thoroughly revealed through researching the microstructure and the microstructure evolution law of the magnetic fluid under the action of the magnetic field. Therefore, it is necessary to study the distribution morphology and structural evolution characteristics of the magnetic fluid from a microscopic perspective. Tang X (Journal of Applied Physics,2000,87 (5): 2634-2638) of Southern Illinois university, USA observes the microstructure morphology of magnetic fluid particle chains under the action of a magnetic field and in the presence or absence of extrusion, and researches find that the magnetic particles are arranged along the direction of the magnetic field under the action of the magnetic field to form a single-chain structure, and the single chain is converted into an obvious columnar structure through the extrusion. In addition, the change characteristics of the magnetic fluid particle chain in the stretching process are obtained by utilizing an optical microscopic defect capturing technology by Furst E M and the like (Physical Review E,2000,62 (5): 6916) of Stanford university in the United states, and researches show that the magnetic particles attached to the surface layer of the particle chain can be arranged in the particle chain in the stretching process of the particle chain to form a regular chain structure. 3238 Zxft 3238 Mm W et al (Macromolecular Research,2018,26 (4): 353-358) at universityA novel magnetic fluid is prepared by ultrasonic emulsification, and its dispersoid is Fe 3 O 4 The coated polystyrene hybrid particles are used for observing and researching the microstructure of the magnetofluid by using a scanning electron microscope. The magnetic field model was used to dynamically model the soft magnetic particles in the magnetic fluid by using the magnetization model, and the interaction of the solid-liquid two-phase body and the micro-dynamic change process of the solid soft magnetic particles were simulated by using the Lennard-Jones potential, gharibvand A J et al (Journal of the Brazilian Society of Mechanical Sciences and Engineering,2019,41 (2): 103) of the Iranian Shahrood science university.
However, at present, researchers at home and abroad mainly study the microstructure characteristics of the magnetic fluid through technical means such as theoretical analysis, numerical simulation, experimental observation and the like, but the theoretical analysis lacks specific experimental verification; in order to accelerate the simulation speed, the numerical simulation is mostly concentrated on the level of few particles and two-dimensional space, and the accurate evolution characteristic of the microstructure of the magnetofluid is difficult to obtain; the experimental observation mainly adopts two research devices, namely a scanning electron microscope and an optical electron microscope, can only capture the surface layer or the slice tissue structure of a sample, mainly adopts qualitative explanation, has the defects of easy damage to the sample, poor effectiveness, large error and the like, can not obtain accurate whole structure information of the magnetorheological fluid, and can not provide accurate reference basis for researching the macroscopic mechanical properties of the magnetorheological fluid.
The prior art discloses a magnetofluid droplet deformation and separation phenomenon observation experiment system, which comprises a test bed and a plurality of droplet measuring assemblies arranged on the test bed, wherein lifting platforms with adjustable heights are respectively arranged on two sides of each droplet measuring assembly, and a light source device with adjustable height is arranged on the other side of each droplet measuring assembly; one side of one group of the lifting platform is provided with a grating ruler, the lifting platform provided with the grating ruler is provided with a magnet piece solenoid or a laser locator, and the other group of the lifting platform is provided with a high-speed camera shooting and collecting device. The scheme can only observe the deformation and separation rule of the magnetic fluid liquid drops under the action of the magnetic field, and cannot acquire accurate whole structure information of the magnetic rheological fluid.
Disclosure of Invention
The invention mainly aims to provide a fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation method for observing the dynamic characteristics of magnetofluid.
The invention further aims to provide a magnetic fluid dynamic characteristic image observation device assisted by fluorescent carbon quantum dots.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation method comprises the following steps:
s1: preparing fluorescent Carbon Quantum Dots (CQDs);
s2: preparing magnetic fluid;
s3: mixing the fluorescent carbon quantum dots and the magnetic fluid to enable the fluorescent carbon quantum dots to be wrapped on the magnetic fluid to form a magnetic fluid composite functional material;
s4: and placing the magnetic fluid composite functional material in a magnetic field to obtain a dynamic characteristic image of the magnetic fluid composite functional material.
Preferably, the preparation of the fluorescent carbon quantum dot specifically comprises the following steps:
dissolving the ground taxus chinensis leaf powder in quantitative acetone or saturated monoaldehyde and ketone, fully stirring, then adding into a reaction kettle, transferring into an oven for heating reaction, and finally centrifuging, dialyzing and filtering the reacted solution to obtain the red carbon quantum dots.
Preferably, the ratio of the taxus leaf powder to acetone or saturated monoaldehyde to ketone is 1g.
Preferably, the magnetic fluid in step S2 is a composite structure of ferroferric oxide and carbon.
Preferably, the preparation of the composite structure of ferroferric oxide and carbon specifically comprises the following steps:
mixing Fe 3 O 4 Introducing the solution into a beaker containing nitric acid, performing ultrasonic treatment for a period of time, adjusting pH to neutral with distilled water, adding into glucose solution, performing ultrasonic treatment,and finally transferring the solution into a reaction kettle, transferring the solution into a drying oven for heating reaction, and finally carrying out heat preservation, cooling and washing on the reacted solution to finally obtain Fe 3 O 4 @C，Fe 3 O 4 @ C denotes a composite structure of ferroferric oxide and carbon.
Preferably, the Fe 3 O 4 The volume ratio of the solution, nitric acid and glucose solution is 5 3 O 4 The concentration of the solution is 0.5-1.5 mol/L, the concentration of the nitric acid is 0.05-0.5 mol/L, the concentration of the glucose solution is 0.1-1.0 mol/L, the heating temperature in the oven is 180-230 ℃, and the reaction time is 6-12 h.
Preferably, the preparation of the magnetic fluid composite functional material in the step S3 specifically comprises:
and (3) adding the magnetic fluid obtained in the step (S2) into a PDDA solution, mixing, performing ultrasonic treatment, stirring, centrifuging and washing, dispersing the mixed solution into the fluorescent carbon quantum dots obtained in the step (S1), performing centrifugal separation again, and washing to obtain the magnetic fluid composite functional material.
Preferably, the mass fraction of the PDDA solution is 0.1-0.4%, and the volume ratio of the magnetic fluid to the fluorescent carbon quantum dots is 1:1-5:1.
A fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation device is characterized in that the image observation device applies the fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation method, the image observation device comprises an ultraviolet light source, a magnetic fluid composite functional material, a permanent magnet, a microscope lens, a high-speed camera and a computer, wherein:
the ultraviolet light source irradiates on the magnetic fluid composite functional material;
placing the magnetic fluid composite functional material in the magnetic field of the permanent magnet;
the microscope lens is opposite to the permanent magnet, and the high-speed camera acquires the image of the magnetofluid composite functional material through the microscope lens and transmits the image to the computer.
Preferably, the light-emitting wavelength of the ultraviolet light source is 365-405 nm, and the magnetic field intensity of the permanent magnet is 15 mT-30 mT.
Compared with the prior art, the technical scheme of the invention has the beneficial effects that:
1. the CQDs used in the invention have excellent fluorescence property, and the raw materials for preparation are easy to obtain, have low cost, are suitable for large-scale production, are easy to have functionality, have low toxicity and have good biocompatibility.
2. The invention can obtain and accurately observe the evolution characteristics of the microstructure of the magnetofluid, and the experimental sample is not easy to damage in the using process.
Drawings
FIG. 1 is a schematic flow chart of the method of the present invention.
FIG. 2 is Fe 3 O 4 Preparation flow chart of @ C @ CQDs.
FIG. 3 is a schematic view of the apparatus of the present invention.
FIG. 4 is a carbon quantum dot fluorescence emission (PL) spectrum.
Fig. 5 is a Transmission Electron Microscope (TEM) image of carbon quantum dots.
Fig. 6 is a carbon quantum dot X-ray diffraction (XRD) pattern.
FIG. 7 is a perspective view of a magnetic fluid according to one embodiment.
FIG. 8 is example di Fe 3 O 4 The TEM image of @ C @ CQDs.
FIG. 9 shows the example of tri-Fe 3 O 4 XRD pattern of @ C @ CQDs.
FIG. 10 is the example of tri-Fe 3 O 4 @ C @ CQDs Fourier transform Infrared Spectroscopy (FTIR) chart.
In the figure, 1 is an ultraviolet light source, 2 is a magnetic fluid composite functional material, 3 is a permanent magnet, 4 is a microscope lens, 5 is a high-speed camera, and 6 is a computer.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent;
for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product;
it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The technical solution of the present invention is further described with reference to the drawings and the embodiments.
Example 1
A fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation method is shown in figure 1 and comprises the following steps:
s1: preparing fluorescent carbon quantum dots;
s2: preparing magnetic fluid;
s3: mixing the fluorescent carbon quantum dots and the magnetic fluid to enable the fluorescent carbon quantum dots to be wrapped on the magnetic fluid to form a magnetic fluid composite functional material;
s4: and placing the magnetic fluid composite functional material in a magnetic field to obtain a dynamic characteristic image of the magnetic fluid composite functional material.
In this embodiment, the fluorescent carbon quantum dots are prepared in step S1, specifically:
as shown in fig. 2, 1g of ground taxus chinensis leaf powder is dissolved in 10ml of acetone, fully stirred, then added into a reaction kettle, transferred into an oven for heating reaction at the reaction temperature of 120 ℃ for 5h, and finally the reacted solution is subjected to centrifugation, dialysis and filtration to obtain CQDs of dark red light;
the PL spectrum of the fluorescence emission of the fluorescent carbon quantum dot is shown in FIG. 4, the TEM image of the fluorescent carbon quantum dot is shown in FIG. 5, and the XRD image of the fluorescent carbon quantum dot is shown in FIG. 6.
Preparing magnetic fluid in step S2, specifically:
as shown in FIG. 2, 5ml of ferroferric oxide Fe with the concentration of 0.5mol/L 3 O 4 Introducing into a beaker containing 1ml of nitric acid with the concentration of 0.05mol/L, performing ultrasonic treatment for a period of time, adjusting the pH value to be neutral by using distilled water, adding 10ml of glucose solution with the concentration of 0.1mol/L, performing ultrasonic treatment, transferring the solution into a reaction kettle, heating in the reaction kettle, and heating at the temperatureHeating for 6h at 180 ℃, and finally performing heat preservation, cooling and washing to obtain Fe 3 O 4 @C；
The magnetic fluid composite functional material in the step S3 specifically comprises the following steps:
as shown in FIG. 2, fe 3 O 4 Adding @ C into 10ml PDDA solution containing 0.1% of mass fraction and 10ml of volume, mixing, performing ultrasonic treatment, stirring, centrifuging, washing, dispersing 5ml of mixed solution into 5ml of CQDs, performing centrifugal separation again, and washing to obtain the magnetic fluid composite functional material Fe 3 O 4 @C@CQDs。
In a specific implementation process, a fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation device is disclosed, as shown in fig. 3, the image observation device applies the above-mentioned fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation method, and the image observation device includes an ultraviolet light source 1, a magnetic fluid composite functional material 2, a permanent magnet 3, a microscope lens 4, a high-speed camera 5 and a computer 6, wherein:
the ultraviolet light source 1 irradiates the magnetic fluid composite functional material 2;
placing the magnetic fluid composite functional material 2 in the magnetic field of the permanent magnet 3;
the microscope lens 4 is over against the permanent magnet 3, and the high-speed camera 5 acquires the image of the magnetofluid composite functional material 2 through the microscope lens 4 and transmits the image to the computer 6.
In this embodiment, the high-speed camera and the ultraviolet light source are turned on, the intensity of the ultraviolet light source is adjusted, the emission wavelength of the ultraviolet light source is 365nm, the magnetic field intensity of the permanent magnet is 15mT, the high-speed camera is connected to a computer, and image processing software provided by the high-speed camera is turned on. Will contain the Fe 3 O 4 The container of the @ C @ CQDs solution was placed at a designated location, and the light source and high speed camera were adjusted to the best shooting conditions. After the preparation is finished, observing the Fe by the magnetic field generated by the permanent magnet 3 O 4 The dynamics of @ C @ CQDs are shown in FIG. 7. Then theAnd adjusting the shooting speed and the shutter speed, selecting the end point for triggering, and then selecting for shooting and recording. And after the selection trigger signal is input, the selection is finished, the required picture or video is finally exported, and finally, all devices are closed in sequence.
Example 2
A fluorescent carbon quantum dot assisted magnetofluid dynamic characteristic image observation method is shown in figure 1 and comprises the following steps:
s1: preparing fluorescent carbon quantum dots;
s2: preparing magnetic fluid;
s3: mixing the fluorescent carbon quantum dots with a magnetic fluid to enable the fluorescent carbon quantum dots to be wrapped on the magnetic fluid to form a magnetic fluid composite functional material;
s4: and placing the magnetic fluid composite functional material in a magnetic field to obtain a dynamic characteristic image of the magnetic fluid composite functional material.
In this embodiment, the fluorescent carbon quantum dots are prepared in step S1, specifically:
as shown in fig. 2, 1g of ground taxus chinensis leaf powder is dissolved in 20ml of acetone, fully stirred, then added into a reaction kettle, transferred into an oven for heating reaction at the reaction temperature of 140 ℃ for 6 hours, and finally the reacted solution is subjected to centrifugation, dialysis and filtration to obtain CQDs of dark red light;
the magnetic fluid is prepared in the step S2, and the method specifically comprises the following steps:
as shown in FIG. 2, 10ml of ferroferric oxide Fe with the concentration of 0.5mol/L 3 O 4 Introducing into a beaker containing 2ml of nitric acid with the concentration of 0.05mol/L, performing ultrasonic treatment for a period of time, adjusting the pH value to be neutral by using distilled water, adding 20ml of glucose solution with the concentration of 0.1mol/L, performing ultrasonic treatment, transferring the solution into a reaction kettle, heating at 180 ℃ for 6 hours, performing heat preservation, cooling and washing for the last time to finally obtain Fe 3 O 4 @C；
The magnetic fluid composite functional material in the step S3 specifically comprises the following steps:
as shown in FIG. 2, fe 3 O 4 @ C plusAdding into 10ml PDDA solution with mass fraction of 0.1%, mixing, ultrasonic treating, stirring, centrifuging, washing, dispersing 10ml mixed solution into 5ml CQDs, centrifuging again, and washing to obtain the magnetic fluid composite functional material Fe 3 O 4 @C@CQDs；
Fe of the present example 3 O 4 TEM image of @ C @ CQDs is shown in FIG. 8.
In a specific implementation process, a fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation device is disclosed, as shown in fig. 3, the image observation device applies the above-mentioned fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation method, and the image observation device includes an ultraviolet light source 1, a magnetic fluid composite functional material 2, a permanent magnet 3, a microscope lens 4, a high-speed camera 5 and a computer 6, wherein:
the ultraviolet light source 1 irradiates the magnetic fluid composite functional material 2;
placing the magnetic fluid composite functional material 2 in the magnetic field of the permanent magnet 3;
the microscope lens 4 is over against the permanent magnet 3, and the high-speed camera 5 acquires the image of the magnetofluid composite functional material 2 through the microscope lens 4 and transmits the image to the computer 6.
In this embodiment, the high-speed camera and the ultraviolet light source are turned on, the intensity of the ultraviolet light source is adjusted, the emission wavelength of the ultraviolet light source is selected to be 380nm, the magnetic field intensity of the permanent magnet is selected to be 20mT, the high-speed camera is connected to a computer, and image processing software carried by the high-speed camera is turned on. Will contain the Fe 3 O 4 The container of @ C @ CQDs solution is placed at a designated place, and the light source and the high-speed camera are adjusted to the optimum shooting conditions. After the preparation is finished, observing the Fe through the magnetic field generated by the permanent magnet 3 O 4 Dynamic characteristics of @ C @ CQDs. Then adjusting the shooting speed and the shutter speed, selecting the trigger of the termination point, and then selecting shooting and recording. After the selection trigger signal is input, the selection is finished, finally the required picture or video is derived, finally, the selection trigger signal is input, and the selection trigger signal is output according to the picture or videoTurning off each device a second time.
Example 3
A fluorescent carbon quantum dot assisted magnetofluid dynamic characteristic image observation method is shown in figure 1 and comprises the following steps:
s1: preparing fluorescent carbon quantum dots;
s2: preparing magnetic fluid;
s3: mixing the fluorescent carbon quantum dots with a magnetic fluid to enable the fluorescent carbon quantum dots to be wrapped on the magnetic fluid to form a magnetic fluid composite functional material;
s4: and placing the magnetic fluid composite functional material in a magnetic field to obtain a dynamic characteristic image of the magnetic fluid composite functional material.
In this embodiment, the fluorescent carbon quantum dots are prepared in step S1, specifically:
as shown in fig. 2, 1g of grinded taxus chinensis leaf powder is dissolved in 30ml of acetone, fully stirred, then added into a reaction kettle, transferred into an oven for heating reaction at 160 ℃ for 6 hours, and finally the reacted solution is centrifuged, dialyzed and filtered, thereby obtaining the CQDs of dark red light;
the magnetic fluid is prepared in the step S2, and the method specifically comprises the following steps:
as shown in FIG. 2, 15ml of ferroferric oxide Fe with the concentration of 0.5mol/L 3 O 4 Introducing into a beaker containing 3ml of nitric acid with the concentration of 0.05mol/L, performing ultrasonic treatment for a period of time, adjusting the pH value to be neutral by using distilled water, adding 30ml of glucose solution with the concentration of 0.1mol/L, performing ultrasonic treatment, transferring the solution into a reaction kettle, heating at 180 ℃ for 6 hours, performing heat preservation, cooling and washing for the last time to finally obtain Fe 3 O 4 @C；
The magnetic fluid composite functional material in the step S3 specifically comprises the following steps:
as shown in FIG. 2, fe 3 O 4 Adding @ C into 10ml PDDA solution with the mass fraction of 0.1%, mixing, performing ultrasonic treatment, stirring, centrifuging, washing, and dispersing 15ml of mixed solution into 5mlThe CQDs is centrifuged again and washed to obtain the magnetic fluid composite functional material Fe 3 O 4 @C@CQDs；
Fe prepared in this example 3 O 4 TEM image of @ C @ CQDs is shown in FIG. 9, fe prepared in this example 3 O 4 FIG. 10 shows FTIR spectrum of Fourier transform infrared spectrum of @ C @ CQDs.
In a specific implementation process, a fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation device is disclosed, as shown in fig. 3, the image observation device applies the above-mentioned fluorescent carbon quantum dot-assisted magnetic fluid dynamic characteristic image observation method, and the image observation device includes an ultraviolet light source 1, a magnetic fluid composite functional material 2, a permanent magnet 3, a microscope lens 4, a high-speed camera 5 and a computer 6, wherein:
the ultraviolet light source 1 irradiates the magnetic fluid composite functional material 2;
placing the magnetic fluid composite functional material 2 in the magnetic field of the permanent magnet 3;
the microscope lens 4 is over against the permanent magnet 3, and the high-speed camera 5 acquires the image of the magnetofluid composite functional material 2 through the microscope lens 4 and transmits the image to the computer 6.
In this embodiment, the high-speed camera and the ultraviolet light source are turned on, the intensity of the ultraviolet light source is adjusted, the emission wavelength of the ultraviolet light source is selected to be 400nm, the magnetic field intensity of the permanent magnet is selected to be 40mT, the high-speed camera is connected to a computer, and image processing software carried by the high-speed camera is turned on. Will contain the Fe 3 O 4 The container of the @ C @ CQDs solution was placed at a designated location, and the light source and high speed camera were adjusted to the best shooting conditions. After the preparation is finished, observing the Fe by the magnetic field generated by the permanent magnet 3 O 4 Dynamics of @ C @ CQDs. Then adjusting the shooting speed and the shutter speed, selecting the end point trigger, and then selecting the shooting and recording. And after the selection trigger signal is input, the selection is finished, the required picture or video is finally exported, and finally, all devices are closed in sequence.
The same or similar reference numerals correspond to the same or similar parts;
the terms describing positional relationships in the drawings are for illustrative purposes only and are not to be construed as limiting the patent;
it should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. A fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation method is characterized by comprising the following steps:
s1: preparing fluorescent carbon quantum dots;
s2: preparing magnetic fluid;
s3: mixing the fluorescent carbon quantum dots with a magnetic fluid to enable the fluorescent carbon quantum dots to be wrapped on the magnetic fluid to form a magnetic fluid composite functional material;
s4: and placing the magnetic fluid composite functional material in a magnetic field to obtain a dynamic characteristic image of the magnetic fluid composite functional material.
2. The method for observing the magnetic fluid dynamic characteristic image assisted by the fluorescent carbon quantum dot according to claim 2, wherein the preparation of the fluorescent carbon quantum dot specifically comprises the following steps:
dissolving the ground taxus chinensis leaf powder in quantitative acetone or saturated monoaldehyde and ketone, fully stirring, then adding into a reaction kettle, transferring into an oven for heating reaction, and finally centrifuging, dialyzing and filtering the reacted solution to obtain the red carbon quantum dots.
3. The method for observing the magnetohydrodynamic characteristic image assisted by fluorescent carbon quantum dots according to claim 2, wherein the ratio of the usage amount of the yew leaf powder to acetone or saturated monoaldehyde to ketone is 1g, 10ml to 130ml, the heating temperature in an oven is 120-220 ℃, and the reaction time is 6-12 h.
4. The method for observing fluorescence carbon quantum dot-assisted magnetofluid dynamic characteristic images according to claim 1, wherein the magnetofluid in the step S2 is a composite structure of ferroferric oxide and carbon.
5. The method for observing magnetic fluid dynamic characteristic images assisted by fluorescent carbon quantum dots according to claim 4, wherein the preparation of the composite structure of ferroferric oxide and carbon specifically comprises the following steps:
mixing Fe 3 O 4 Introducing the solution into a beaker containing nitric acid, performing ultrasonic treatment for a period of time, adjusting the pH value to be neutral by using distilled water, adding the solution into a glucose solution, performing ultrasonic treatment, transferring the solution into a reaction kettle, transferring the solution into an oven, performing heating reaction, and performing heat preservation, cooling and washing on the reacted solution to obtain Fe 3 O 4 @C，Fe 3 O 4 @ C denotes a composite structure of ferroferric oxide and carbon.
6. The method for observing magnetofluid dynamic characteristic images assisted by fluorescent carbon quantum dots according to claim 5, wherein the Fe is Fe 3 O 4 The volume ratio of the solution, nitric acid and glucose solution is 5 3 O 4 The concentration of the solution is 0.5-1.5 mol/L, the concentration of the nitric acid is 0.05-0.5 mol/L, the concentration of the glucose solution is 0.1-1.0 mol/L, the heating temperature in the oven is 180-230 ℃, and the reaction time is 6-12 h.
7. The method for observing the magnetic fluid dynamic characteristic image assisted by the fluorescent carbon quantum dots according to claim 1, wherein the preparation of the magnetic fluid composite functional material in the step S3 specifically comprises the following steps:
and (3) adding the magnetic fluid obtained in the step (S2) into a PDDA solution, mixing, performing ultrasonic treatment, stirring, centrifuging and washing, dispersing the mixed solution into the fluorescent carbon quantum dots obtained in the step (S1), performing centrifugal separation again, and washing to obtain the magnetic fluid composite functional material.
8. The method for observing fluorescence carbon quantum dot-assisted magnetofluid dynamic characteristic images as claimed in claim 8, wherein the mass fraction of the poly diallyldimethylammonium chloride PDDA solution is 0.1-0.4%, and the volume ratio of the magnetofluid to the fluorescence carbon quantum dots is 1:1-5:1.
9. A fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation device is characterized in that the image observation device applies the fluorescent carbon quantum dot-assisted magnetofluid dynamic characteristic image observation method of any one of claims 1 to 8, and comprises an ultraviolet light source (1), a magnetofluid composite functional material (2), a permanent magnet (3), a microscope lens (4), a high-speed camera (5) and a computer (6), wherein:
the ultraviolet light source (1) irradiates the magnetic fluid composite functional material (2);
placing the magnetic fluid composite functional material (2) in the magnetic field of the permanent magnet (3);
the microscope lens (4) is over against the permanent magnet (3), and the high-speed camera (5) acquires an image of the magnetofluid composite functional material (2) through the microscope lens (4) and transmits the image to the computer (6).
10. The device for observing magnetohydrodynamic features assisted by fluorescent carbon quantum dots according to claim 9, wherein the ultraviolet light source (1) has a light-emitting wavelength of 365-405 nm, and the permanent magnet (3) has a magnetic field strength of 15 mT-30 mT.
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