CN115386466A

CN115386466A - Multi-path independent microorganism culture detection system

Info

Publication number: CN115386466A
Application number: CN202211015795.4A
Authority: CN
Inventors: 陈志浩; 王立田; 刘允伟
Original assignee: Anhui Wanyi Science and Technology Co Ltd
Current assignee: Anhui Wanyi Science and Technology Co Ltd
Priority date: 2022-08-23
Filing date: 2022-08-23
Publication date: 2022-11-25

Abstract

The invention discloses a multi-path independent microorganism culture detection system which comprises a culture module, a culture solution storage bottle, a second power pump, a first multi-path shunt valve seat, a second multi-path shunt valve seat, a waste liquid bottle and a waste discharge port, wherein the culture module comprises 5-9 culture dishes and can be used for culturing different types of microorganisms, the culture solution storage bottle is used for storing culture solution required by microorganism culture, the second power pump is used for the circulation of liquid in the whole equipment, the first multi-path shunt valve seat is connected with the second power pump and the culture module, the second multi-path shunt valve seat is connected with the culture module and the waste liquid bottle, the waste liquid bottle is used for receiving the waste liquid of the culture module, and the waste discharge port is connected with the waste liquid bottle.

Description

Multi-path independent microorganism culture detection system

Technical Field

The invention belongs to the technical field of microbial culture and signal detection, and particularly relates to a multi-path independent microbial culture detection system.

Background

At present, researchers at home and abroad develop new methods for rapidly detecting escherichia coli, mainly comprising an enzyme substrate method, a polymerase chain method, an immunoassay method, an electrochemical method, a fluorescence method and the like, although the methods shorten the detection time and simplify the detection process, the detection time is longer, and a single experiment result needs about 24 hours, so that a method for rapidly detecting the intestinal flora, which can maintain high sensitivity and high selectivity, is urgently needed.

Disclosure of Invention

In order to solve the above-mentioned deficiencies in the prior art, the present invention provides a multiplex independent microorganism culture detection system, which simplifies the process and improves the detection efficiency.

The invention also aims to provide a multi-path independent microorganism culture detection system.

According to an embodiment of the present invention, a multi-channel independent microorganism culture detection system comprises: the culture module comprises 5-9 culture dishes, preferably 6 culture dishes which are respectively a first culture dish, a second culture dish, a third culture dish, a fourth culture dish, a fifth culture dish and a sixth culture dish and can be used for culturing different types of microorganisms; the culture dish comprises: a housing; the upper liquid level meter is used for measuring the liquid level of the culture dish; a gas port, the culture dish having a gas port; the culture dish is provided with a liquid inlet and a liquid outlet; the reactor is arranged in the culture dish and is communicated with the gas port, the liquid inlet and the liquid outlet; a fluorescent signal excitation light source for exciting a generated fluorescent substance; an absorbance detection light source for detecting an absorbance of the solution; the device comprises a first photoelectric detector and a second photoelectric detector, wherein the first photoelectric detector and the second photoelectric detector are used for detecting a fluorescence signal excitation light source and an absorbance detection light source; the culture solution storage bottle is used for storing culture solution required by microorganism culture; a second power pump for circulation of liquid throughout the apparatus; the first multi-way shunt valve seat is connected with the second power pump and the culture module; the second multi-way shunt valve seat is connected with the culture module and the waste liquid bottle; the first multi-way diverter valve seat is provided with valve ports 2a, 2b, 2c, 2d, 2e and 2f which are correspondingly connected with liquid inlets of 6 culture dishes, and the second multi-way diverter valve seat is provided with

valve ports

3a, 3b, 3c, 3d, 3e and 3f which are correspondingly connected with liquid outlets of 6 culture dishes; the waste liquid bottle is used for receiving waste liquid of the culture module; and the waste discharge port is connected with the waste liquid bottle.

According to the multi-channel independent microorganism culture detection system in the embodiment of the invention, the second power pump firstly pumps out the culture solution in the culture solution storage bottle, the culture solution is injected into the first culture dish through the 2a valve port on the first multi-channel shunt valve seat after four hours, the culture solution is injected into the second culture dish through the 2b valve port on the first multi-channel shunt valve seat after four hours, the culture solution is injected into the third culture dish through the 2c valve port on the first multi-channel shunt valve seat after four hours, the culture solution is injected into the fourth culture dish through the 2d valve port on the first multi-channel shunt valve seat after four hours, the culture solution is injected into the fifth culture dish through the 2e valve port on the first multi-channel shunt valve seat after four hours, the culture solution is injected into the sixth culture dish through the 2f valve port on the first multi-channel shunt valve seat after four hours, at the moment, when the culture solution in the sixth culture dish starts to be detected, the result is output, the culture solution is treated through the 2f valve port on the first multi-channel shunt valve seat after four hours, the culture solution bottle is discharged, and the waste solution bottle is discharged into the sixth culture dish after 24 hours, and the culture solution bottle is discharged, and the culture dish is discharged, and the waste solution bottle is discharged.

Optionally, according to the multi-channel independent microorganism culture detection system in the embodiment of the present invention, when the number of the culture dishes is 5, according to the above operation method, a result may be output every four hours and forty-eight minutes, and a 24-hour all-day cycle is performed, where the specific number needs to be set by an operator according to an actual situation.

In some embodiments, further comprising: the reagent bottle group comprises at least six reagent bottles which are respectively used for storing a water sample, disinfectant, pure water, air and two standby reagent bottles; the first power pump is used for guiding the liquid reagent in the reagent bottle group into the culture module, and the second power pump is used for guiding the gas reagent in the reagent bottle group into the culture module; the third multi-way flow distributing valve seat is connected with the first power pump and the reagent bottles in the reagent bottle group, and valve ports of the third multi-way flow distributing valve seat correspond to the reagent bottles in the reagent bottle group in one-to-one mode in the same number; first three-way valve, second three-way valve and third three-way valve, first three-way valve, second three-way valve and third three-way valve are used for switching the connecting channel of first power pump or second power pump, before the culture dish cultivates, and accessible second power pump will cultivate required gas from importing into the culture dish in, and inside the required liquid reagent of the required leading-in culture dish of will cultivateing of accessible first power pump, guaranteed good cultivation environment.

In some embodiments, further comprising: and the metering tube is used for metering the liquid entering the culture module, and quantitatively operating the culture solution in the process of injecting the culture solution into the culture dish.

In some embodiments, further comprising: and the pinch valve is arranged between the waste liquid bottle and the waste discharge port, the pinch valve closes the liquid outlet of the waste liquid bottle in the waste gas discharge process, and the pinch valve is opened in the waste liquid discharge process.

According to the multi-channel independent microorganism culture detection system disclosed by the embodiment of the invention, the detection interval requirement can be ensured, the equipment volume can be reduced, the detection result can be rapidly output all weather, the detection flow is simplified, and the detection efficiency is improved.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

FIG. 1 is a schematic view of a flow path structure of a multi-path independent microorganism culture detection system in an embodiment of the invention.

FIG. 2 is a schematic structural view of a culture dish according to an embodiment of the present invention.

In the reference symbols:

1. a culture module;

1a. A first culture dish; 1b. A second culture dish; 1c. A third culture dish; 1d, a fourth culture dish; 1e. fifth petri dish; 1f. sixth Petri dish;

101. a housing; 102. a reactor; 103. an upper liquid level meter; 104. a gas port; 105. a first photodetector; 106. a second photodetector; 107. a liquid outlet; 108. a liquid inlet; 109. an absorbance detection light source; 110. a fluorescence signal excitation light source;

2. a first multi-way diverter valve seat; 3. a second multi-way diverter valve seat; 4. a waste liquid bottle; 5. a waste discharge port; 6. a second power pump; 7. a culture solution storage bottle; 8. a first three-way valve; 9. a second three-way valve; 10. a metering tube;

11. a reagent bottle group; 11a. Water sample bottle; 11b, disinfectant bottle; 11c, a pure water bottle; 11d, air bottle; 11e, a standby bottle; 11f, a standby bottle;

12. a third multi-way diverter valve seat; 13. a first power pump; 14. a third three-way valve; 15. and a gas valve.

Detailed Description

The present application will be described in further detail with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not to be construed as limiting the invention. It should be noted that, for convenience of description, only the portions related to the present invention are shown in the drawings.

The specific structure of the culture dish according to the embodiment of the invention is described below with reference to fig. 2.

As shown in fig. 2, a culture dish according to an embodiment of the present invention includes: the device comprises a shell 101, an upper liquid level meter 103, a gas port 104, a liquid inlet 108, a liquid outlet 107, a reactor 102, a fluorescence signal excitation light source 110, an absorbance detection light source 109, a first photoelectric detector 105, a second photoelectric detector 106 and an optical fiber 111, wherein the upper liquid level meter 103 is used for measuring the liquid level of a culture dish, the culture dish is provided with the gas port 104 and is provided with the liquid inlet 108 and the liquid outlet 107, the liquid inlet 108 and the liquid outlet 107 are preferably arranged at the bottom of the culture dish, the culture liquid is fed into the bottom, flora is automatically and uniformly mixed in the culture liquid along with the rise of the liquid level of the culture liquid due to the gathering of the flora at the bottom of the culture dish, so as to achieve the purpose of automatic uniform mixing, the reactor 102 is arranged inside the culture dish, the reactor 102 is communicated with the gas port 104, the liquid inlet 108 and the liquid outlet 107, the optical fiber 111 penetrates through the shell 101 and the reactor 102 and is arranged inside the reactor 102, and the optical fiber 111 is connected with the optical fiber detector outside the device, the device is used for detecting a fluorescence signal excitation light source 110, the fluorescence signal excitation light source 110 is used for exciting a generated fluorescent substance, the first photoelectric detector 105 and the second photoelectric detector 106 are used for detecting an absorbance detection light source 109, a culture solution enters a culture dish to culture microorganisms, the gas port 104 is used for introducing gas in the culture process, the liquid inlet 108 is used for introducing a liquid reagent in the culture process, the microorganisms and the reagent react to generate a specific product in the reactor 102, the fluorescence signal excitation light source 110 can excite the fluorescent substance to generate fluorescent light with a specific wavelength, the fluorescent substance can be detected by the optical fiber detector through the optical fiber 111 and output a detection result, the absorbance detection light source 109 can be detected by the first photoelectric detector 105 and the second light source detector and output a detection result, the penetrating and arranging direction of the optical fiber 111 is preferably orthogonal to the light source emitted by the absorbance detection light source 109, the fluorescence signal excitation light source 110 and the absorbance detection light source 109 have the same direction, and the second photodetector 106 and the absorbance detection light source 110 are symmetrically arranged, so that the optical fiber 111 and the second photodetector 106 do not interfere with each other, and the detection accuracy is improved.

As shown in fig. 1, the multiple independent microorganism culture detection system in the embodiment of the present invention includes a culture module 1, a culture solution storage bottle 7, a second power pump 6, a first multiple-way shunt valve seat 2, a second multiple-way shunt valve seat 3, a waste liquid bottle 4, a waste discharge port 5 and a metering tube 10, wherein the culture module 1 includes 5-9 culture dishes, preferably 6 culture dishes, as described above, and can be used for culturing different types of microorganisms, the culture solution storage bottle 7 is used for storing culture solution required for culturing microorganisms, the second power pump 6 is used for circulating liquid in the whole apparatus, the first multiple-way shunt valve seat 2 connects the second power pump 6 with the culture module 1, the second multiple-way shunt valve seat 3 connects the culture module 1 with the waste liquid bottle 4, the valve ports on the first multiple-way shunt valve seat 2 and the second multiple-way shunt valve seat 3 are the same as the culture dishes in the culture module 1, and are in one-to-one correspondence, the first multi-way flow-dividing valve seat 2 is provided with a valve port 2a, a valve port 2b, a valve port 2c, a valve port 2d, a valve port 2e and a valve port 2f which are correspondingly connected with the liquid inlets 108 of 6 culture dishes, the second multi-way flow-dividing valve seat 3 is provided with a valve port 3a, a valve port 3b, a valve port 3c, a valve port 3d, a valve port 3e and a valve port 3f which are correspondingly connected with the liquid outlets 107 of the 6 culture dishes, a waste liquid bottle 4 is used for receiving waste liquid of the culture module 1, a waste discharge port 5 is connected with a waste liquid bottle 4, a metering tube 10 is used for metering liquid entering the culture module 1, the culture liquid is quantitatively operated in the process of injecting the culture liquid into the culture dishes, the culture liquid in the culture liquid storage bottle 7 is firstly sucked out by a second power pump 6 and is metered by the metering tube 10, and is selected by an interface on the first multi-way flow-dividing valve seat 2, every four hours, pour into the culture solution into a culture dish, after the culture solution is poured into to last culture dish, the inside data of outputtable first culture dish, through the interface selection on the first many-way reposition of redundant personnel disk seat 2, the waste liquid of leading into waste liquid bottle 4 in the first culture dish, finally discharges through waste discharge opening 5, pours into the culture solution into again to first culture dish to analogize to, every four hours, just can export once data, realize all-weather circulation and detect.

In some embodiments, the culture module 1 includes five culture dishes, and similarly, the culture solution is injected once every four hours and forty-eight minutes, after the last culture solution is injected, the detection data of the first culture dish is output, after the detection is completed, the waste liquid is discharged, the culture solution is injected into the first culture dish again, and so on, the data can be output once every four hours and forty-eight minutes, so as to realize all-weather cycle detection.

In some embodiments, the valve ports on the first multi-way diverter valve seat 2 and the second multi-way diverter valve seat 3 can be opened simultaneously, so that a plurality of culture dishes in the culture module 1 can be cultured simultaneously, the detection is performed jointly, the data is output jointly, and the accuracy of the detection data is improved.

The multiple independent microorganism culture detection system according to embodiments of the present invention, in some embodiments, further comprises: the reagent bottle group 11, the first power pump 13, the third multi-way shunt valve seat 12, the reagent bottle inside the reagent bottle group 11 is used for storing the reagent which can change the culture environment, the reagent bottle group 11 includes at least six reagent bottles which are respectively used for storing water sample, disinfectant, pure water, air and two standby reagent bottles, the first power pump 13 is used for leading the liquid reagent inside the reagent bottle group 11 into the culture module 1, the second power pump 6 is used for leading the gas reagent inside the reagent bottle group 11 into the culture module 1, the third multi-way shunt valve seat 12 is connected with the first power pump 13 and the reagent bottle inside the reagent bottle group 11, and the valve port of the third multi-way shunt valve seat 12 is the same with the number of the reagent bottles in the reagent bottle group 11, and is in one-to-one correspondence, 6 valve ports of the third multi-way shunt valve seat 12 are respectively connected with the liquid outlets 107 of 6 reagent bottles in the reagent bottle group 11, the first three-way valve 8, the second three-way valve 9 and the third three-way valve 14 are used for switching the connecting passages of the first power pump 13 or the second power pump 6, before the culture dish is cultured, the gas required for culture can be introduced into the culture dish from the inlet through the second power pump 6, the liquid reagent required for culture can be introduced into the culture dish through the first power pump 13, and a good culture environment can be ensured.

Example 1

As shown in fig. 1, the multiple independent microorganism culture detection system in the embodiment of the present invention includes a culture module 1, a culture solution storage bottle 7, a second power pump 6, a first multi-way diverter valve seat 2, a second multi-way diverter valve seat 3, a waste liquid bottle 4, a waste outlet 5 and a metering tube 10.

The culture module 1 comprises 6 culture dishes, a second power pump 6 is used for flowing and rotating liquid in the whole device, a first multi-way shunt valve seat 2 is connected with the second power pump 6 and the culture module 1, a second multi-way shunt valve seat 3 is connected with the culture module 1 and a waste liquid bottle 4, the number of valve ports on the first multi-way shunt valve seat 2 and the second multi-way shunt valve seat 3 is the same as that of the culture dishes in the culture module 1, and the valve ports are in one-to-one correspondence with the culture dishes in the culture module 1, the first multi-way shunt valve seat 2 is provided with a valve port 2a, a valve port 2b, a valve port 2c, a valve port 2d, a valve port 2e and a valve port 2f which are correspondingly connected with liquid inlets 108 of the 6 culture dishes, the second multi-way shunt valve seat 3 is provided with a valve port 3a, a valve port 3b, a valve port 3c, a valve port 3d, a valve port 3e and a waste liquid outlet 107 of the 6 dishes, the waste liquid bottle 4 is used for receiving waste liquid of the culture dish, the waste liquid discharge port 5 is connected with the waste liquid bottle 4, and the metering tube 10 is used for metering liquid entering the culture module 1, and quantitatively injecting the culture liquid into the culture dish.

Example 2

As shown in fig. 2, the culture dish in example 1 of the present invention comprises: the device comprises a housing 101, an upper liquid level meter 103, a gas port 104, a liquid inlet 108, a liquid outlet 107, a reactor 102, a fluorescence signal excitation light source 110, an absorbance detection light source 109, a first photoelectric detector 105 and a second photoelectric detector 106.

Example 3

The multi-channel independent microorganism culture detection system according to embodiment 1 is different from embodiment 1 in that the culture module 1 includes 5 culture dishes, the first multi-channel split-flow valve seat 2 is provided with a valve port 2a, a valve port 2b, a valve port 2c, a valve port 2d and a valve port 2e which are correspondingly connected with the liquid inlets 108 of the 5 culture dishes, and the second multi-channel split-flow valve seat 3 is provided with a valve port 3a, a valve port 3b, a valve port 3c, a valve port 3d and a valve port 3e which are correspondingly connected with the liquid outlets 107 of the 5 culture dishes.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.

The foregoing description is only exemplary of the preferred embodiments of the application and is illustrative of the principles of the technology employed. It will be appreciated by a person skilled in the art that the scope of the invention as referred to in the present application is not limited to the embodiments with a specific combination of the above-mentioned features, but also covers other embodiments with any combination of the above-mentioned features or their equivalents without departing from the inventive concept. For example, the above features may be replaced with (but not limited to) features having similar functions disclosed in the present application.

Other technical features than those described in the specification are known to those skilled in the art, and are not described herein in detail in order to highlight the innovative features of the present invention.

Claims

1. A multiplex independent microbial culture detection system, comprising:

the culture module (1), the culture module (1) comprises 5-9 culture dishes, and can be used for culturing different kinds of microorganisms;

the culture solution storage bottle (7) is used for storing a culture solution required by microorganism culture;

a second power pump (6), wherein the second power pump (6) is used for circulating liquid in the whole equipment;

the first multi-way shunt valve seat (2), and the first multi-way shunt valve seat (2) is connected with the second power pump (6) and the culture module (1);

the second multi-way shunt valve seat (3), the second multi-way shunt valve seat (3) is connected with the culture module (1) and the waste liquid bottle (4);

the waste liquid bottle (4), the waste liquid bottle (4) is used for receiving the waste liquid of the culture module (1); and

the waste discharge port (5), the waste discharge port (5) is connected with the waste liquid bottle (4).

2. The multi-channel independent microbial culture detection system of claim 1, wherein the valve ports on the first multi-channel shunt valve seat (2) and the second multi-channel shunt valve seat (3) are respectively the same in number as the culture dishes in the culture module (1) and are in one-to-one correspondence with the culture dishes in the culture module.

3. The multiple independent microbial culture detection system of claim 2, wherein a pinch valve is arranged between the waste liquid bottle (4) and the waste outlet (5).

4. The multiple stand-alone microorganism culture detection system of claim 3, further comprising:

a reagent bottle group (11), wherein the reagent bottle group (11) is used for storing reagents capable of changing culture environments; and

a first powered pump (13), the first powered pump (13) being configured to introduce liquid reagents inside the group of reagent bottles (11) into the incubation module (1), the second powered pump (6) being configured to introduce gaseous reagents inside the group of reagent bottles (11) into the incubation module (1).

5. The multiple stand-alone microorganism culture detection system of claim 4, further comprising:

a metering tube (10), the metering tube (10) for metering liquid into the incubation module (1).

6. The multiple stand-alone microorganism culture detection system of claim 4, further comprising:

and the air valve (15) is a three-way valve, and the air valve (15) is respectively connected with the culture module (1), the second power pump (6) and the waste discharge port (5).

7. The multiple stand-alone microorganism culture detection system of claim 4, further comprising:

the third multi-way flow distributing valve seat (12) is connected with the first power pump (13) and the reagent bottles in the reagent bottle group (11), and valve ports of the third multi-way flow distributing valve seat (12) correspond to the reagent bottles in the reagent bottle group (11) in the same number one by one.

8. The multi-channel independent microorganism culture detection system of claim 7, further comprising:

the power pump comprises a first three-way valve (8), a second three-way valve (9) and a third three-way valve (14), wherein the first three-way valve (8), the second three-way valve (9) and the third three-way valve (14) are used for switching the connecting passages of a first power pump (13) or a second power pump (6).

9. The multiple stand-alone microorganism culture detection system of claim 1, wherein the culture dish comprises:

a housing (101);

the upper liquid level meter (103), the upper liquid level meter (103) is used for measuring the liquid level of the culture dish;

a gas port (104), the culture dish having a gas port (104);

a liquid inlet (108) and a liquid outlet (107), the culture dish is provided with a liquid inlet (108) and a liquid outlet (107);

the reactor (102) is arranged in the culture dish, and the reactor (102) is communicated with the gas port (104), the liquid inlet (108) and the liquid outlet (107);

a fluorescence signal excitation light source (110), wherein the fluorescence signal excitation light source (110) is used for exciting the generated fluorescent substance;

an absorbance detection light source (109), wherein the absorbance detection light source (109) is used for detecting the absorbance of the solution;

a first photodetector (105) and a second photodetector (106), the first photodetector (105) and the second photodetector (106) being configured to detect a photometric detection light source (109);

and the optical fiber (111) penetrates through the shell (101) and the reactor (102), is arranged in the reactor (102), and is used for detecting the fluorescence signal excitation light source (110).

10. The multiple stand-alone microorganism culture detection system of claim 9, further comprising:

a heater disposed on an inner wall of the housing (101);

a temperature sensor, the detection head of which is positioned inside the reactor (102) and is used for detecting the temperature inside the reactor (102).