CN115386445A - Preparation method of natural hirudin wine - Google Patents

Preparation method of natural hirudin wine Download PDF

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CN115386445A
CN115386445A CN202211168155.7A CN202211168155A CN115386445A CN 115386445 A CN115386445 A CN 115386445A CN 202211168155 A CN202211168155 A CN 202211168155A CN 115386445 A CN115386445 A CN 115386445A
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hirudin
leeches
adult
blood
wine
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文泽沂
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • C12G3/05—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/10—Anti-acne agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/02—Non-specific cardiovascular stimulants, e.g. drugs for syncope, antihypotensives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/02—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material
    • C12H1/06—Precipitation by physical means, e.g. by irradiation, vibrations
    • C12H1/063—Separation by filtration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The invention discloses a preparation method of natural hirudin wine, which relates to the field of hirudin wine preparation, and comprises the following steps: s1, selecting a part of adult gilt-edged leeches, placing the adult gilt-edged leeches in a separate pool, purifying the adult gilt-edged leeches without feeding, performing saliva induction operation on the purified adult gilt-edged leeches in a sterile environment, filtering saliva obtained by the induction operation after the induction operation is completed, removing impurities after filtering and filtering, purifying hirudin, and sterilizing for later use. The invention can play a role in regulating blood pressure in two directions. Preventing myocardial infarction and cerebral infarction, hirudin can eliminate mural thrombus of coronary artery and free thrombus of systemic artery, expand, soften blood vessel, relieve stenosis degree of coronary artery and increase elasticity of blood vessel wall, thereby effectively preventing myocardial infarction and cerebral infarction.

Description

Preparation method of natural hirudin wine
Technical Field
The invention relates to the field of hirudin wine preparation, and in particular relates to a preparation method of natural hirudin wine.
Background
The leech variety Poecilobdella manillensis with the highest hirudin content, namely the golden-edged leech, is screened and bred, the material basis for extracting the hirudin with high efficiency and low cost is laid, the hirudin is creatively and successfully applied to the development of functional foods and cosmetics for the first time, and the natural hirudin is the strongest natural antithrombin substance found in the world so far, and has unique curative effects and effects on human sub-health hypertension, hyperglycemia and cerebrovascular diseases, particularly cerebral infarction and myocardial infarction patients. The natural hirudin is extracted from a pure natural animal source, and the LD50 of 50ATU/g of hirudin freeze-dried powder is more than 10g/kg through experimental study, and the hirudin freeze-dried powder belongs to an actual non-toxic level. And the medicament has no mutation effect, has no obvious bleeding effect, has the dosage required by the actual anticoagulation effect which is far larger than the actually used dosage in clinical research, has the safe dosage of 1mg/kg, and has no anaphylactic reaction and toxic reaction. The natural hirudin is specifically combined with thrombin, and the proteolysis of the thrombin is inhibited, so that fibrinogen cannot be converted into fibrin, the coagulation of the fibrin is prevented, and the strong anticoagulation effect is achieved. Meanwhile, hirudin can also neutralize thrombin combined with fibrin, has thrombolytic effect on both arteriovenous thrombus plaques and can also prevent new thrombus formation. The natural hirudin is low molecular polypeptide, has the mass of only 7000 daltons, can be quickly absorbed by a human body to play a quick role and has the capability of passing through the blood brain barrier of the human body, and the blood stasis embolism of cerebral vessels, which is difficult to achieve by common medicines, also has the specific dissolving and decomposing effects. The natural hirudin has definite action of inhibiting blood plate aggregation by inhibiting blood plate activation induced by thrombin, can obviously reduce the specific viscosity of whole blood and the specific viscosity of blood plasma, improve blood microcirculation, remove metabolic waste in blood circulation in time and preserve the healthy environment of blood.
In the traditional preparation method of natural hirudin, the content of hirudin secreted by the blood sucking leech is low, the content of active units is low, the traditional leech dry powder on the market is an extract raw material under the standardized and purified reproduction condition, the traditional leech dry powder is green, environment-friendly, free of toxic and side effects, free of pesticide residues, incapable of meeting the use requirements in safety standard, and poor in the integral use effect.
Therefore, the person skilled in the art provides a method for preparing natural hirudin wine to solve the problems mentioned in the background art.
Disclosure of Invention
The invention aims to provide a preparation method of natural hirudin wine, which aims to solve the problems that in the traditional preparation method of natural hirudin provided by the background technology, the content of hirudin secreted by blood sucking leeches is low, the content of active units is low, the traditional dried leech powder on the market is an extract raw material under the standardized and purified reproduction condition, the use requirement cannot be met due to environmental protection, no toxic or side effect, no pesticide residue and safety standard, and the integral use effect is poor.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of natural hirudin wine comprises the following steps:
s1, selecting a part of adult gilt-edged leeches, placing the adult gilt-edged leeches in a separate pool, purifying the adult gilt-edged leeches without feeding, performing saliva induction operation on the purified adult gilt-edged leeches in a sterile environment, filtering saliva obtained by the induction operation after the induction operation is completed, removing impurities after filtering and filtering, purifying hirudin, and sterilizing for later use.
S2, a step of separating and purifying the rana japonica extract, which is to select sulfuric acid to separate and purify the rana japonica extract according to three protein precipitation methods, namely a fractional precipitation salting-out method, an organic solvent precipitation method and a precipitation method, to carry out coarse separation and purification on the salivary secretion of the rana japonica by utilizing the fractional precipitation salting-out of the mercuric acid, and then to select an ion exchange chromatography method, a gel filtration chromatography method and an affinity chromatography method to carry out refining and purification.
And S3, a step of fusing the base wine and hirudin, namely fusing ATU (adenosine triphosphate) of the purified hirudin into the base wine in a corresponding amount according to different proportions, wherein the hirudin belongs to small molecular polypeptides and can be directly absorbed by a human body and also can be directly fused into water and wine.
And S4, a step of blending base liquor and aged liquor, which is to blend the blended base liquor into the aged liquor with a corresponding quantity, wherein the blended liquor generates a small amount of precipitate, the liquor is uniformly mixed and then is stored at normal temperature for more than 5 days, and then is filtered by a special filter for white liquor until the liquor is clear, and then is bottled to leave the factory.
As a preferred embodiment of the present invention: in the material selecting treatment step, the time length of the purification process of non-feeding adult leeches is about one half month, and in the saliva inducing operation of the purified adult leeches, the purified adult leeches with golden edges are placed into the small pig intestines after cleaning and disinfection, so that the adult leeches with golden edges are induced to spit out saliva.
As a preferred embodiment of the present invention: in the step of material selection treatment, the storage temperature of the purified hirudin cannot be higher than 45 ℃, and the frequency of purifying the hirudin from adult leeches with golden edges is 7 times at most.
As a preferred embodiment of the present invention: in the step of separating and purifying the frogesin, 35 to 55 percent saturation (NH) can be selected as sulfuric acid according to a fractional precipitation salting-out method 4 ) 2 SO 4 。
As a preferred embodiment of the present invention: in the step of blending the base liquor and the aged liquor, the prepared base liquor is required to be diluted and prepared within 30 days, and the temperature in the production process is not more than 45 ℃.
Compared with the prior art, the invention has the beneficial effects that:
the invention relates to a preparation method of natural hirudin wine, wherein hirudin extracted from Poecilobdella manillensis has the following effects: the problem of myocardial and cerebral infarction is solved: anticoagulation and thrombolysis: the natural hirudin has a unique structure, is easy to penetrate through a blood brain barrier and quickly enters the brain to play a unique effect; meanwhile, the anticoagulation and thrombolysis effects of the heparin-heparin composite anticoagulant and thrombolytic drug do not need the participation of auxiliary factors, and the dissolved thrombus does not relapse, so that the effect of the heparin-heparin composite anticoagulant and thrombolytic drug is far better than that of the heparin-heparin composite anticoagulant and thrombolytic drug which is commonly used clinically at present. In conclusion, the traditional Chinese medicine composition has unique effect on preventing and conditioning the myocardial and cerebral infarction problems. Removing the vascular plaque: natural hirudin is the only natural substance capable of removing plaque in the world at present. This operation, of course, requires a specialist and unique hirudin formulation and expert guidance to be able to complete. The problem of three high is solved: blood pressure, blood lipid and blood sugar reduction: the hirudin can obviously reduce the blood pressure, cholesterol, triglyceride and blood sugar of SHR rats, thereby proving that the hirudin has the functions of reducing blood pressure, blood fat and blood sugar. The sub-health problem is solved: improvement of hemorheology: the hirudin has effects of reducing blood viscosity, accelerating blood flow, improving blood circulation, and promoting metabolism by reducing blood rheology index, whole blood viscosity, and erythrocyte aggregation index of normal and blood stasis model rat. The functions of relieving alcoholism, detoxifying and resisting inflammation are as follows: hirudin can rapidly decompose acetaldehyde which is an intermediate product harmful to alcohol oxidation, and has obvious anti-inflammatory effect on acute and chronic inflammation. Improving immunity of human body. The natural hirudin can be taken for more than three years for a long time, so that the immunity of the human body can be well improved, and the fever caused by continuous cold is reduced or even not caused. Hirudin has good regulating and repairing effects on nephritis, nephrotic syndrome and uremia. The beauty treatment effect is as follows: the hirudin has the functions of automatically and directly permeating, reducing blood viscosity, accelerating blood flow speed, dredging capillary vessels, improving blood circulation and promoting metabolism, so that nutrient substances such as nutrients, moisture, oxygen and the like can fully reach elastic fibers and collagen fibers through the transportation of blood, the normal functions of the fibers are maintained, and the skin is fine and smooth, can contract large pores, and particularly has unique effects on acne, facial pigmentation, skin scar repair, whitening, freckle removal, alopecia prevention and treatment and hair regeneration promotion, thereby fundamentally solving various skin problems and achieving the purpose of blood beauty. The blood viscosity is reduced, the hirudin actively permeates into the blood of a human body and then is in a negative ion state, so that aggregated red blood cells and platelets are dispersed, excessive lipid components, free radicals, toxic scales and the like in the blood are removed, and the blood viscosity is effectively reduced. The hirudin can be used for conditioning sub-health, and can be used for improving microcirculation of organs of the whole body by diluting blood, removing blood toxin, improving blood viscosity, accelerating blood circulation and the like, so that various sub-health problems can be well solved. Regulating blood lipid abnormality, hirudin can effectively decompose cholesterol, and remove excessive lipid component in blood, so as to regulate blood lipid index, reduce lipid component deposition on blood vessel wall, and prevent atherosclerosis. The hirudin can reduce blood viscosity, accelerate blood circulation, clear blood waste, recover blood vessel elasticity, keep blood pressure in a normal and stable range, and has the function of regulating blood pressure in two directions. The hirudin can eliminate mural thrombus of coronary artery and free thrombus of systemic artery, expand and soften blood vessel, reduce stenosis degree of coronary artery and increase elasticity of vessel wall, thereby effectively preventing myocardial infarction and cerebral infarction, the poecilobdella manillensis is commonly called leech with golden edge in Guangxi, the content of hirudin secreted by salivary gland is about 5 times higher than that of hirudin secreted by other blood sucking leeches, the natural hirudin secreted by the salivary gland of the poecilobdella manillensis is extracted from living bodies of the poecilobdella manillensis in the world at present, the content of active unit is highest, and the extract raw material under the standardized and purified reproduction condition is green, environment-friendly, free of toxic and side effects, free of pesticide residue and safety standard far higher than that of the traditional leech dry body powder on the market.
Detailed Description
In the embodiment of the invention, a preparation method of natural hirudin wine comprises the following steps:
s1, selecting a part of adult gilt-edged leeches, placing the adult gilt-edged leeches in a separate pool, purifying the adult gilt-edged leeches without feeding, performing saliva induction operation on the purified adult gilt-edged leeches in a sterile environment, filtering saliva obtained by the induction operation after the induction operation is completed, removing impurities after filtering and filtering, purifying hirudin, and sterilizing for later use.
S2, a step of separating and purifying the rana japonica extract, which is to select sulfuric acid to separate and purify the rana japonica extract according to three protein precipitation methods, namely a fractional precipitation salting-out method, an organic solvent precipitation method and a precipitation method, to carry out coarse separation and purification on the salivary secretion of the rana japonica by utilizing the fractional precipitation salting-out of the mercuric acid, and then to select an ion exchange chromatography method, a gel filtration chromatography method and an affinity chromatography method to carry out refining and purification.
And S3, a step of fusing the base wine and hirudin, namely fusing ATU (adenosine triphosphate) of the purified hirudin into the base wine in a corresponding amount according to different proportions, wherein the hirudin belongs to small molecular polypeptides and can be directly absorbed by a human body and also can be directly fused into water and wine.
And S4, a step of blending base liquor and aged liquor, which is to blend the blended base liquor into the aged liquor with a corresponding quantity, wherein the blended liquor generates a small amount of precipitate, the liquor is uniformly mixed and then is stored at normal temperature for more than 5 days, and then is filtered by a special filter for white liquor until the liquor is clear, and then is bottled to leave the factory.
The hirudin extracted from Poecilobdella manillensis has the following effects: the problem of myocardial and cerebral infarction is solved: anticoagulation and thrombolysis: the natural hirudin has a unique structure, is easy to penetrate through a blood brain barrier and quickly enters the brain to play a unique effect; meanwhile, the anticoagulation and thrombolysis effects of the compound do not need the participation of auxiliary factors, the dissolved thrombus does not relapse, and the effect of the compound is far better than that of heparin which is a commonly used anticoagulation medicament in clinic at present. In conclusion, the traditional Chinese medicine composition has unique effect on preventing and conditioning the myocardial and cerebral infarction problems. Removing the vascular plaque: natural hirudin is the only natural substance capable of removing plaque in the world at present. This operation, of course, requires a specialist and unique hirudin formulation and expert guidance to be able to complete. The problem of three highs is solved: blood pressure, blood lipid and blood sugar reduction: hirudin can significantly reduce blood pressure, cholesterol, triglyceride and blood sugar of SHR rats, thereby proving that hirudin has effects of lowering blood pressure, reducing blood lipid and lowering blood sugar. The sub-health problem is solved: improvement of hemorheology: the hirudin has effects of reducing blood viscosity, accelerating blood flow, improving blood circulation, and promoting metabolism by reducing blood rheology index, whole blood viscosity, and erythrocyte aggregation index of normal and blood stasis model rat. The effects of relieving alcoholism, detoxifying and resisting inflammation are as follows: hirudin can rapidly decompose acetaldehyde which is an intermediate product harmful to alcohol oxidation, and has obvious anti-inflammatory effect on acute and chronic inflammation. Improving immunity of human body. The natural hirudin can be taken for more than three years for a long time, so that the immunity of the human body can be well improved, and the fever caused by continuous cold is reduced or even not caused. Hirudin has good regulating and repairing effects on nephritis, nephrotic syndrome and uremia. The beauty treatment effect is as follows: the hirudin has the functions of automatically and directly permeating, reducing blood viscosity, accelerating blood flow speed, dredging capillary vessels, improving blood circulation and promoting metabolism, so that nutrient substances such as nutrients, moisture, oxygen and the like can fully reach elastic fibers and collagen fibers through the transportation of blood, the normal functions of the fibers are maintained, and the skin is fine and smooth, can contract large pores, and particularly has unique effects on acne, facial pigmentation, skin scar repair, whitening, freckle removal, alopecia prevention and treatment and hair regeneration promotion, thereby fundamentally solving various skin problems and achieving the purpose of blood beauty. The blood viscosity is reduced, the hirudin actively permeates into the blood of a human body and then is in a negative ion state, so that aggregated red blood cells and platelets are dispersed, excessive lipid components, free radicals, toxic scales and the like in the blood are removed, and the blood viscosity is effectively reduced. The hirudin can be used for conditioning sub-health, and can be used for improving microcirculation of organs of the whole body by diluting blood, removing blood toxin, improving blood viscosity, accelerating blood circulation, etc., thereby solving various sub-health problems. Regulating blood lipid abnormality, hirudin can effectively decompose cholesterol, and remove excessive lipid component in blood, so as to regulate blood lipid index, reduce lipid component deposition on blood vessel wall, and prevent atherosclerosis. The hirudin can reduce blood viscosity, accelerate blood circulation, clear blood waste, recover blood vessel elasticity, keep blood pressure in a normal and stable range, and has the function of regulating blood pressure in two directions. Preventing myocardial infarction and cerebral infarction, hirudin can eliminate mural thrombus of coronary artery and free thrombus of systemic artery, expand and soften blood vessel, relieve stenosis degree of coronary artery and increase elasticity of blood vessel wall, thereby effectively preventing myocardial infarction and cerebral infarction.
In the material selecting treatment step, the time length of the purification process of non-feeding adult leeches is about one half month, and in the saliva inducing operation of the purified adult leeches with golden edges, the purified adult leeches with golden edges are placed into the small intestines of the pigs after cleaning and disinfection, so as to induce the adult leeches to spit out saliva. In the step of material selection treatment, the storage temperature of the purified hirudin cannot be higher than 45 ℃, and the time for purifying the hirudin from adult leeches with golden edges is 7 times at most. In the step of separating and purifying the ranophanin, the sulfuric acid is subjected to fractional precipitation salting-out methodOptionally 35-55% saturation (NH) 4 ) 2 SO 4 . In the step of blending the base wine and the aged wine, the prepared base wine is required to be diluted and prepared within 30 days, and the temperature in the production process is not more than 45 ℃.
The hirudin secreted by salivary glands of the poecilobdella manillensis is about 5 times higher than that secreted by other leeches in Guangxi, and the natural hirudin secreted by the salivary glands of the poecilobdella manillensis is obtained by extracting the raw materials from living bodies of the poecilobdella manillensis in the world at present, so that the content of active units is the highest, and the extract raw materials under the standardized and purified reproduction conditions are green, environment-friendly, free of toxic and side effects, free of pesticide residues and far higher in safety standard than the traditional dried leech powder on the market.
The working principle of the invention is as follows: a preparation method of natural hirudin wine comprises the following steps:
s1, selecting a part of adult gilt-edged leeches, placing the adult gilt-edged leeches in a separate pool, purifying the adult gilt-edged leeches without feeding, performing saliva induction operation on the purified adult gilt-edged leeches in a sterile environment, filtering saliva obtained by the induction operation after the induction operation is completed, removing impurities after filtering and filtering, purifying hirudin, and sterilizing for later use. In the material selecting treatment step, the time length of the purification process of non-feeding adult leeches is about one half month, and in the saliva inducing operation of the purified adult leeches with golden edges, the purified adult leeches with golden edges are placed into the small intestines of the pigs after cleaning and disinfection, so as to induce the adult leeches to spit out saliva. In the step of material selection treatment, the storage temperature of the purified hirudin cannot be higher than 45 ℃, and the maximum number of times of purifying the hirudin from adult leeches with golden edges is 7.
S2, a step of separating and purifying the frogazepin, which is to select sulfuric acid to separate and purify the frogazepin by three protein precipitation methods, namely a fractional precipitation salting-out method, an organic solvent precipitation method and a precipitation method, to carry out coarse separation and purification on the salivary secretion of the frogazepin by utilizing the fractional precipitation salting-out of the sulfuric acid, and then to select an ion exchange chromatography method and a gel filtration chromatography methodAnd purifying by affinity chromatography. In the step of separating and purifying the ranophanin, 35-55% saturation (NH) can be selected as sulfuric acid by fractional precipitation salting-out method 4 ) 2 SO 4 。
And S3, a step of fusing the base wine and hirudin, namely fusing ATU (adenosine triphosphate) of the purified hirudin into the base wine in a corresponding amount according to different proportions, wherein the hirudin belongs to small molecular polypeptides and can be directly absorbed by a human body and also can be directly fused into water and wine.
And S4, a step of blending base liquor and aged liquor, which is to blend the blended base liquor into the aged liquor with a corresponding quantity, wherein the blended liquor generates a small amount of precipitate, the liquor is uniformly mixed and then is stored at normal temperature for more than 5 days, and then is filtered by a special filter for white liquor until the liquor is clear, and then is bottled to leave the factory. In the step of blending the base liquor and the aged liquor, the prepared base liquor is required to be diluted and prepared within 30 days, and the temperature in the production process is not more than 45 ℃.
The hirudin extracted from Poecilobdella manillensis has the following effects: the problem of myocardial and cerebral infarction is solved: anticoagulation and thrombolysis: the natural hirudin has a unique structure, is easy to penetrate through a blood brain barrier and quickly enters the brain to play a unique effect; meanwhile, the anticoagulation and thrombolysis effects of the heparin-heparin composite anticoagulant and thrombolytic drug do not need the participation of auxiliary factors, and the dissolved thrombus does not relapse, so that the effect of the heparin-heparin composite anticoagulant and thrombolytic drug is far better than that of the heparin-heparin composite anticoagulant and thrombolytic drug which is commonly used clinically at present. In conclusion, the traditional Chinese medicine composition has unique effect on preventing and conditioning the myocardial and cerebral infarction problems. Removing the vascular plaque: natural hirudin is the only natural substance capable of removing plaque in the world at present. This operation, of course, requires a specialist and unique hirudin formulation and expert guidance to be able to be carried out. The problem of three highs is solved: blood pressure, blood lipid and blood sugar reduction: hirudin can significantly reduce blood pressure, cholesterol, triglyceride and blood sugar of SHR rats, thereby proving that hirudin has effects of lowering blood pressure, reducing blood lipid and lowering blood sugar. The sub-health problem is solved: improvement of hemorheology: the hirudin has effects of reducing blood viscosity, accelerating blood flow, improving blood circulation, and promoting metabolism by reducing blood rheology index, whole blood viscosity, and erythrocyte aggregation index of normal and blood stasis model rat. The functions of relieving alcoholism, detoxifying and resisting inflammation are as follows: hirudin can rapidly decompose intermediate acetaldehyde harmful to alcohol oxidation, and has obvious anti-inflammatory effect on acute and chronic inflammation. Improving immunity of human body. The natural hirudin is taken for more than three years for a long time, so that the immunity of a human body can be well improved, and the fever caused by continuous cold is reduced or even avoided. Hirudin has good regulating and repairing effects on nephritis, nephrotic syndrome and uremia. The beauty treatment effect is as follows: hirudin has effects of independently and directly permeating, reducing blood viscosity, accelerating blood flow speed, dredging capillary vessel, improving blood circulation, and promoting metabolism, so that nutrients such as nutrients, moisture and oxygen can fully reach elastic fiber and collagen fiber through blood transportation, normal functions of the fibers are maintained, skin is fine and smooth, and large pores are shrunk, especially acne, facial pigmentation, skin scar repair, whitening, freckle removal, alopecia prevention and treatment and hair regeneration promotion are achieved, and therefore various skin problems are fundamentally solved, and the purpose of blood beautifying is achieved. The blood viscosity is reduced, the hirudin actively permeates into the blood of a human body and then is in a negative ion state, so that aggregated red blood cells and platelets are dispersed, excessive lipid components, free radicals, toxic scales and the like in the blood are removed, and the blood viscosity is effectively reduced. The hirudin can be used for conditioning sub-health, and can be used for improving microcirculation of organs of the whole body by diluting blood, removing blood toxin, improving blood viscosity, accelerating blood circulation, etc., thereby solving various sub-health problems. Regulating blood lipid abnormality, hirudin can effectively decompose cholesterol, and remove excessive lipid component in blood, thereby regulating blood lipid index, reducing lipid component deposition on blood vessel wall, and preventing atherosclerosis. The hirudin can reduce blood viscosity, accelerate blood circulation, clear blood waste, recover blood vessel elasticity, keep blood pressure in a normal and stable range, and has the function of regulating blood pressure in two directions. Preventing myocardial infarction and cerebral infarction, hirudin can eliminate mural thrombus of coronary artery and free thrombus of systemic artery, expand and soften blood vessel, relieve stenosis degree of coronary artery and increase elasticity of blood vessel wall, thereby effectively preventing myocardial infarction and cerebral infarction. The hirudin secreted by salivary glands of the poecilobdella manillensis is up to about 5 times higher than that secreted by other bloodsucking leeches in Guangxi, and the natural hirudin secreted by the salivary glands of the poecilobdella manillensis is obtained by extracting the raw materials from the living bodies of the poecilobdella manillensis in the world at present, so that the content of the active units is highest, and the extract raw materials under the standardized and purified reproduction conditions are green, environment-friendly, free of toxic and side effects, free of pesticide residues and far higher in safety standard than the traditional dried leech powder on the market.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims (5)

1. The preparation method of the natural hirudin wine is characterized by comprising the following steps of:
s1, selecting a part of adult gilt-edged leeches, placing the adult gilt-edged leeches in a separate pool, purifying the adult gilt-edged leeches without feeding, performing saliva induction operation on the purified adult gilt-edged leeches in a sterile environment, filtering saliva obtained by the induction operation after the induction operation is completed, removing impurities after filtering and filtering, purifying hirudin, and sterilizing for later use.
S2, a step of separating and purifying the frogavidin, which is to select sulfuric acid to separate and purify the frogavidin by three protein precipitation methods, namely a fractional precipitation salting-out method, an organic solvent precipitation method and a precipitation method, use the fractional precipitation salting-out of the sulfuric acid to carry out coarse separation and purification on the frogavidin secretion, and then select an ion exchange chromatography method, a gel filtration chromatography method and an affinity chromatography method to carry out refining and purification.
And S3, a step of fusing the base wine and hirudin, namely fusing ATU (adenosine triphosphate) of the purified hirudin into the base wine in a corresponding amount according to different proportions, wherein the hirudin belongs to small molecular polypeptides and can be directly absorbed by a human body and also can be directly fused into water and wine.
And S4, a step of blending base liquor and aged liquor, which is to blend the blended base liquor into the aged liquor with a corresponding quantity, wherein the blended liquor generates a small amount of precipitate, the liquor is uniformly mixed and then is stored at normal temperature for more than 5 days, and then is filtered by a special filter for white liquor until the liquor is clear, and then is bottled to leave the factory.
2. The method of claim 1, wherein in the step of selecting the material, the period of time for which adult leeches are not fed is about one and a half months, and in the step of inducing saliva in the purified adult leeches, the purified adult leeches with golden border are placed in the cleaned and sterilized small intestine of a pig, so as to induce the adult leeches to spit out the saliva.
3. The method of claim 1, wherein the purified hirudin obtained in the step of selecting the material is stored at a temperature not higher than 45 degrees, and the number of times that an adult leech is purified is 7 at most.
4. The method for preparing natural hirudin wine as claimed in claim 1, wherein in the step of separating and purifying the frogmuiin, the sulfuric acid is selected from (NH) with 35% -55% saturation by fractional precipitation salting-out method 4 ) 2 SO 4 。
5. The method according to claim 1, wherein the blending step of the base wine and the aged wine requires the prepared base wine to be diluted within 30 days, and the temperature of the production process does not exceed 45 ℃.
CN202211168155.7A 2022-09-23 2022-09-23 Preparation method of natural hirudin wine Pending CN115386445A (en)

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