CN115369042B - Acremonium 320 and application thereof - Google Patents
Acremonium 320 and application thereof Download PDFInfo
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- CN115369042B CN115369042B CN202110541696.9A CN202110541696A CN115369042B CN 115369042 B CN115369042 B CN 115369042B CN 202110541696 A CN202110541696 A CN 202110541696A CN 115369042 B CN115369042 B CN 115369042B
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- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 title claims abstract description 22
- 241000228417 Sarocladium strictum Species 0.000 claims abstract description 10
- 230000008635 plant growth Effects 0.000 claims abstract description 7
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 230000001737 promoting effect Effects 0.000 claims abstract description 5
- 239000002207 metabolite Substances 0.000 claims description 16
- 241000196324 Embryophyta Species 0.000 claims description 10
- 244000061176 Nicotiana tabacum Species 0.000 claims description 7
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 6
- 241000207746 Nicotiana benthamiana Species 0.000 claims description 5
- 239000003337 fertilizer Substances 0.000 claims description 4
- 241000219194 Arabidopsis Species 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims 2
- 240000002791 Brassica napus Species 0.000 claims 1
- 235000011293 Brassica napus Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000001965 increasing effect Effects 0.000 abstract description 11
- 244000005700 microbiome Species 0.000 abstract description 5
- 229930002875 chlorophyll Natural products 0.000 abstract description 4
- 235000019804 chlorophyll Nutrition 0.000 abstract description 4
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 abstract description 4
- 238000009629 microbiological culture Methods 0.000 abstract description 3
- 230000012010 growth Effects 0.000 description 12
- 238000009630 liquid culture Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 7
- 238000001914 filtration Methods 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 240000007124 Brassica oleracea Species 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000012982 microporous membrane Substances 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 108020004463 18S ribosomal RNA Proteins 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108091023242 Internal transcribed spacer Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Mycology (AREA)
- Dentistry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the field of promoting plant growth by microorganisms, and provides Acremonium (Acremonium strictum) 320, the preservation number of which is CGMCC No.21935, the strain is preserved in China general microbiological culture Collection center (CGMCC) for a period of 25 days in 2021, and the address of the strain is CGMCC No.1, the North Chen West road No. 3 in the Korean region of Beijing city, and the preservation number of the strain is CGMCC No.21935. The strain can promote plant growth, the fresh weight, root weight, plant height and stem thickness of the plant are obviously increased, and the chlorophyll content of the leaf is also obviously increased.
Description
Technical Field
The invention belongs to the field of promoting plant growth by microorganisms.
Background
At present, the application of various chemical fertilizers and pesticides is still a main means for increasing the yield and ensuring the income of crops, but the adverse effect of the crop yield is that the ecological environment system is destroyed, and the human health is seriously threatened. The application of chemical fertilizer and pesticide is reduced, which is a necessary path for the development of green agriculture. The beneficial microorganism is utilized to promote the growth of crops, which is one of the effective ways of reducing the application and enhancing the efficiency of chemical fertilizer and pesticide. Research has shown that growth promoting microorganisms can promote plant growth and development and resist environmental stress in various ways such as improving soil structure, decomposing organic matters and minerals, fixing nitrogen, dissolving phosphorus, dissolving potassium, degrading heavy metals, and the like.
Disclosure of Invention
In order to solve the technical problem in the application, the application provides Acremonium (Acremonium strictum) 320 with a preservation number of CGMCC No.21935, which is preserved in China general microbiological culture Collection center (CGMCC) in 2021, 3 and 25 days, and has an address of CGMCC No.1, 3 and a preservation number of CGMCC No.21935.
The strain can promote plant growth, the fresh weight, root weight, plant height and stem thickness of the plant are obviously increased, and the chlorophyll content of the leaf is also obviously increased.
Drawings
FIG. 1 is a colony morphology of Acremonium 320.
FIG. 2 is a graph of the growth of Arabidopsis thaliana lateral root promoted by Acremonium 320.
FIG. 3 is a diagram showing the promotion of growth of cabbage by Acremonium 320 metabolite.
FIG. 4 is a graph of the promotion of tobacco growth by Acremonium 320 metabolites.
FIG. 5A diagram of the promotion of growth of N.benthamiana by Acremonium erectum metabolites.
Detailed Description
Example 1
Bacterial strain acquisition
1) The strain was harvested from the medium when we performed plant tissue culture and isolated on potato agar medium PDA (potato 200g, glucose 20g, agar 20 g).
2) Identification of strains
Firstly, extracting fungus DNA, carrying out PCR amplification by fungus universal primers ITS1 (TCCGTAGGTGAACCTGCGG) and ITS4 (TCCTCCGCTTATTGATATGC), carrying out nucleotide sequence determination on PCR products, and comparing the obtained sequences on NCBI (https:// blast.ncbi.lm.nih.gov/blast.cgi), wherein the homology with Acremonium sp.) G4 is up to 99.66%.
After single colony culture, the bacterial colony of the strain is light orange yellow, the surface is slightly moist, hyphae are branched, the hyphae are separated, and spores of the bacterial colony are elliptical with the diameter of about 1.5 mu m and the length of about 4 mu m (figure 1). Through 18S rRNA sequence morphological analysis, the classification name Acremonium strictum is identified, the Chinese corresponding name is Acremonium, the name is 320, and the strain is called 320 for short in the patent. Acremonium (Acremonium strictum) 320 was deposited in China general microbiological culture collection center (CGMCC) at 25 months of 2021, and the deposited number is CGMCC No.21935.
Example 2
Identification of strain functions: the bacterium has the characteristic of obviously promoting plant growth by co-culturing with plants and irrigating with metabolites thereof.
The germinated Arabidopsis seedlings were transferred to 1/2MS solid medium (MS salt 4.3g/L, sucrose 10g/L, agar powder 7.5g/L, pH 5.8), and Acremonium (Acremonium strictum) 320 was inoculated in the lower portion of the dish and placed in a 23℃incubator for cultivation. After 15 days of co-culture, the side roots and leaves of the arabidopsis after the treatment of the acremonium are vigorous in growth, and the length of the side roots is increased by about 2 times of that of a control; the leaves became significantly larger and green (fig. 2).
Example 3
The 320 colonies of Acremonium (Acremonium strictum) cultured in the solid medium were picked up in MS liquid medium (MS salt 4.3g/L, sucrose 10g/L, pH 5.8), placed in a constant temperature shaker at 28℃and cultured with shaking at 200 rpm. After removing mycelia by filtering the liquid culture with 8 layers of gauze for 7 days, centrifuging at 6000rpm for 5min to collect culture solution, filtering with 0.45 μm microporous membrane, and collecting metabolite. Metabolites of fungi were directly irrigated into pakchoi cultivated in soil (Brassica campestris l. Ssp. Chinensis Makino) once every other week. From the first watering, their growth changes were recorded by photographing at intervals. MS liquid culture medium is used as control.
The results showed that the growth of the cabbage plants irrigated with the metabolite was more vigorous than the control. The leaves of the cabbage were significantly increased after 10 days of metabolite treatment. The growth of the cabbage plants after 25 days of metabolite treatment was significantly increased, with plant weight and leaf area approximately 2 times that of the control. (FIG. 3).
Example 4
The 320 colonies of Acremonium (Acremonium strictum) cultured in the solid culture medium are picked up into MS liquid culture medium, placed in a constant temperature shaking table at 28 ℃ and cultured by shaking at 200 rpm. After removing mycelia by filtering the liquid culture with 8 layers of gauze for 7 days, centrifuging at 6000rpm for 5min to collect culture solution, filtering with 0.45 μm microporous membrane, and collecting metabolite. Metabolites of fungi were directly irrigated into tobacco benthamiana (Nicotiana benthamiana) cultivated in soil, once every other week. From the first watering, their growth changes were recorded by photographing at intervals. MS liquid culture medium is used as control.
The results showed that the Nicotiana benthamiana plants irrigated with the metabolite grew more vigorously than the control, and the fresh weight, root weight, plant height, and stem thickness of the plants were all significantly increased, with the plant weight and root weight being approximately 2.5 times that of the control and the plant height and stem thickness being approximately 1.5 times that of the control for 25 days. The chlorophyll content of the leaves also increased significantly (fig. 4).
Example 5
The 320 colonies of Acremonium (Acremonium strictum) cultured in the solid culture medium are picked up into MS liquid culture medium, placed in a constant temperature shaking table at 28 ℃ and cultured by shaking at 200 rpm. After removing mycelia by filtering the liquid culture with 8 layers of gauze for 7 days, centrifuging at 6000rpm for 5min to collect culture solution, filtering with 0.45 μm microporous membrane, and collecting metabolite. Metabolites of fungi were irrigated directly into tobacco (n.tabacum l.) cultivated in soil, once every other week. From the first watering, their growth changes were recorded by photographing at intervals. MS liquid culture medium is used as control.
The results showed that the tobacco plant weight and root weight of the topical acremonium erectum metabolite was approximately 2.5 times that of the control, the plant height was approximately 2 times that of the control, and the stem thickness was approximately 1.5 times that of the control. The chlorophyll content of the leaves also increased significantly (fig. 5).
Sequence listing
<110> institute of microorganisms at national academy of sciences
<120> Acremonium 320 strain and use thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 580
<212> DNA
<213> Acremonium (Acremonium strictum)
<400> 1
ctccgtaggt gaacctgcgg agggatcatt acaaaacgag tgcttcggct ctccaaccca 60
ttgtgaacat acctatcgtt ccctcggcgg gctcagcgcg cggccggcct ccgggccacc 120
gggcgtccgc cggggacaac caaacccgaa tttttcgtgt atatctgagg ggcgaaagcc 180
cgaaaacaaa atgaatcaaa actttcaaca acggatctct tggctctggc atcgatgaag 240
aacgcagcga aatgcgataa gtaatgtgaa ttgcagaatt cagtgaatca tcgaatcttt 300
gaacgcacat tgcgcccgcc ggcactccgg cgggcatgcc tgtccgagcg tcatttcaac 360
cctcagggcc ccctttcggg ggcggcacct ggttctgggg atcagcggcc cttcggggcc 420
cctgtccccc aaattgagtg gcggtcgcgc cgcagcctcc cctgcgtagt agcacacctc 480
gcaccggaga gcggctcggc cacgccgtaa aacccccaat ttttacaggt tgacctcgga 540
tcaggtagga atacccgctg aacttaagca tatcaataag 598
Claims (4)
1. Acremonium (Acremonium strictum) 320 has a preservation number of CGMCC No.21935.
2. Use of acremonium 320 and metabolites thereof according to claim 1 for the manufacture of a plant growth promoting adjuvant, said plants being arabidopsis, brassica napus and tobacco plants.
3. The use according to claim 2, wherein the tobacco plants include nicotiana benthamiana plants and nicotiana benthamiana plants.
4. The use according to claim 2, wherein the adjuvant comprises a fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110541696.9A CN115369042B (en) | 2021-05-18 | 2021-05-18 | Acremonium 320 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110541696.9A CN115369042B (en) | 2021-05-18 | 2021-05-18 | Acremonium 320 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115369042A CN115369042A (en) | 2022-11-22 |
| CN115369042B true CN115369042B (en) | 2024-04-05 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110541696.9A Active CN115369042B (en) | 2021-05-18 | 2021-05-18 | Acremonium 320 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115369042B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990026602A (en) * | 1997-09-25 | 1999-04-15 | 서주원 | New Acremonium sp. Use of MJN1 strain and its extract as pollution-free plant growth promoter |
| CN102363748A (en) * | 2011-09-30 | 2012-02-29 | 中国科学院南海海洋研究所 | New fungus Acremonium sp. DPZ-SYz-2-3 for high efficiency cellulose degradation and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2871245A1 (en) * | 2013-11-08 | 2015-05-13 | Symborg, S.L. | A bacillus subtilis strain deposited under deposit number CECT 8258 and method for protecting or treating plants |
-
2021
- 2021-05-18 CN CN202110541696.9A patent/CN115369042B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990026602A (en) * | 1997-09-25 | 1999-04-15 | 서주원 | New Acremonium sp. Use of MJN1 strain and its extract as pollution-free plant growth promoter |
| CN102363748A (en) * | 2011-09-30 | 2012-02-29 | 中国科学院南海海洋研究所 | New fungus Acremonium sp. DPZ-SYz-2-3 for high efficiency cellulose degradation and application thereof |
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| Publication number | Publication date |
|---|---|
| CN115369042A (en) | 2022-11-22 |
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