CN115368484B - Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics - Google Patents
Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics Download PDFInfo
- Publication number
- CN115368484B CN115368484B CN202211190678.1A CN202211190678A CN115368484B CN 115368484 B CN115368484 B CN 115368484B CN 202211190678 A CN202211190678 A CN 202211190678A CN 115368484 B CN115368484 B CN 115368484B
- Authority
- CN
- China
- Prior art keywords
- peach gum
- gum polysaccharide
- polysaccharide
- preparation
- peach
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000006040 Prunus persica var persica Nutrition 0.000 title claims abstract description 200
- 244000144730 Amygdalus persica Species 0.000 title claims abstract description 199
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 151
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 151
- 150000004676 glycans Chemical class 0.000 title claims abstract description 150
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 239000002537 cosmetic Substances 0.000 title claims abstract description 17
- 150000001413 amino acids Chemical class 0.000 claims abstract description 16
- 239000004475 Arginine Substances 0.000 claims abstract description 7
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 6
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims abstract description 3
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims abstract description 3
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims abstract description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000004472 Lysine Substances 0.000 claims abstract description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims abstract description 3
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 3
- 230000007062 hydrolysis Effects 0.000 claims description 32
- 238000006460 hydrolysis reaction Methods 0.000 claims description 32
- 210000004027 cell Anatomy 0.000 claims description 24
- 230000003110 anti-inflammatory effect Effects 0.000 claims description 14
- 239000012528 membrane Substances 0.000 claims description 13
- 238000000265 homogenisation Methods 0.000 claims description 12
- 238000000926 separation method Methods 0.000 claims description 9
- 230000035755 proliferation Effects 0.000 claims description 8
- 230000001737 promoting effect Effects 0.000 claims description 6
- 210000002950 fibroblast Anatomy 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 abstract description 7
- 239000003513 alkali Substances 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 27
- 230000000694 effects Effects 0.000 description 25
- 230000004663 cell proliferation Effects 0.000 description 22
- 238000000034 method Methods 0.000 description 18
- 238000002474 experimental method Methods 0.000 description 15
- 239000007788 liquid Substances 0.000 description 15
- 230000008961 swelling Effects 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 239000000843 powder Substances 0.000 description 13
- 239000000706 filtrate Substances 0.000 description 8
- 239000000413 hydrolysate Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 239000007864 aqueous solution Substances 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000002791 soaking Methods 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 238000007865 diluting Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 238000001728 nano-filtration Methods 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000007619 statistical method Methods 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 102000004889 Interleukin-6 Human genes 0.000 description 3
- 108090001005 Interleukin-6 Proteins 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000003833 cell viability Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000012224 working solution Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 206010018291 Gingival swelling Diseases 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229910017053 inorganic salt Inorganic materials 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 206010007027 Calculus urinary Diseases 0.000 description 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 240000005809 Prunus persica Species 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000001516 cell proliferation assay Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000008269 hand cream Substances 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 229940100601 interleukin-6 Drugs 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000010951 particle size reduction Methods 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000000475 sunscreen effect Effects 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000009283 thermal hydrolysis Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 208000008281 urolithiasis Diseases 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Birds (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Epidemiology (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to the technical field of peach gum processing, in particular to a preparation method of peach gum polysaccharide, application of the peach gum polysaccharide and cosmetics. The preparation method comprises the following steps: mixing peach gum with amino acid, and hydrolyzing to obtain peach gum polysaccharide; the amino acid is one or more of lysine, arginine and histidine. Compared with the prior art, the preparation method provided by the invention uses amino acid to replace a strong alkali raw material, so that the safety performance of peach gum polysaccharide is ensured from the source, and the safety performance of the follow-up related cosmetics is further improved.
Description
Technical Field
The invention relates to the technical field of peach gum processing, in particular to a preparation method of peach gum polysaccharide, application of the peach gum polysaccharide and cosmetics.
Background
Peach gum is also called peach gum, and is light red or light yellow semitransparent colloid secreted by the trunk of Rosaceae plant such as peach, plum, etc. after being injured or infected by microorganism, and the solid formed by air drying or other dehydration on tree becomes original peach gum. The peach gum is composed of polysaccharide and its derivatives, a small amount of protein and impurities, and has medicinal and health promoting effects. The traditional Chinese medicine is clinically used for treating the symptoms of urolithiasis, bloody stranguria, dysentery, diabetes and the like, and modern researches prove that the peach gum polysaccharide has the effects of reducing blood sugar, reducing blood fat, regulating immunity, promoting gastrointestinal peristalsis and treating burn.
The swelling but insoluble nature of crude peach gum in water greatly limits its wide application. How to hydrolyze and process peach gum becomes one of the main factors restricting the wide application of peach gum extract. The current industrial hydrolysis methods of peach gum mainly comprise a thermal hydrolysis method, an acid hydrolysis method and an alkaline hydrolysis method. The addition and residue of reagents such as strong acids and bases during hydrolysis may affect the properties of the final product.
Disclosure of Invention
Therefore, the invention aims to provide a preparation method of peach gum polysaccharide, application of the preparation method and cosmetics, wherein amino acid is used for replacing strong alkali raw materials, so that the safety performance of the peach gum polysaccharide is ensured from the source, and the safety performance of the follow-up related cosmetics is further improved.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a preparation method of peach gum polysaccharide comprises the following steps: mixing peach gum with amino acid, and hydrolyzing to obtain peach gum polysaccharide; the amino acid is one or more of lysine, arginine and histidine.
In the present invention, the amino acid is preferably arginine, and more preferably cosmetic grade arginine;
the peach gum is raw peach gum or peach gum powder; peach gum powder can be made by methods purchased or well known to those skilled in the art;
the preparation method of the peach gum powder comprises the following steps:
mixing the raw peach gum with water, soaking and expanding, then drying the peach gum, and finally crushing the dried peach gum to obtain peach gum powder; the raw peach gum is mixed with water according to the solid-to-liquid ratio of 1 (15-30), and the preferable ratio is 1:20; the temperature of the soaking and expanding is 20-45 ℃, preferably 25 ℃; the soaking and expanding time is 16-28 h, and is preferably 24h; the drying is drying or freeze drying; the invention preferably breaks up the dried peach gum and screens it with a 100 mesh screen. In the embodiment of the invention, the influence of the solid-liquid ratio and the swelling temperature on the swelling time of the original peach gum is examined, so as to explore the preparation process of the peach gum polysaccharide with higher efficiency, namely swelling the peach gum by water under the conditions of the solid-liquid ratio of 1:20 and the temperature of 25 ℃.
In the invention, the temperature of the hydrolysis is 50-60 ℃;
the pH value of the hydrolysis is 8-10, preferably 10;
the hydrolysis time is 6 to 24 hours, preferably 12 hours.
In the invention, the peach gum is mixed with the solution of amino acid; the mass ratio of the peach gum to the amino acid solution is (0.5-2) to 100, and the pH of the amino acid solution is 8-10. The invention explores the influence of hydrolysis conditions on the performance of peach gum polysaccharide extract, particularly the influence of peach gum content and hydrolysis time on the degree of hydrolysis under alkaline conditions, particularly the morphological change of peach gum solution, and explores the optimal hydrolysis conditions, namely pH value is 10, and hydrolysis temperature is 50-60 ℃.
In the present invention, the above preparation method further comprises: homogenizing the hydrolyzed peach gum polysaccharide under high pressure; the pressure of the high-pressure homogenization is 100-500 Bar, and is preferably 500Bar; the number of times of high-pressure homogenization is preferably 3. High-pressure homogenization can obtain peach gum polysaccharide with more uniform texture, concentrated molecular weight and smaller molecular weight.
The high-pressure homogenization treatment further reduces the molecular weight of the peach gum polysaccharide, and is beneficial to the preparation of the low-molecular weight peach gum polysaccharide. The molecular weight test results in the embodiment of the invention show that the polysaccharide with the molecular weight smaller than 100K daltons in the peach gum polysaccharide prepared by the invention accounts for more than 90 percent, and the polysaccharide with the molecular weight smaller than 10K daltons accounts for more than 60 percent, which indicates that the prepared peach gum polysaccharide has small molecular weight and concentrated distribution.
In the present invention, the above preparation method further comprises: separating and post-treating the hydrolyzed peach gum polysaccharide; the separation adopts membrane separation equipment with molecular weight of 10K and 100K; the post-treatment adopts a nanofiltration membrane with a molecular weight of 300. The nanofiltration membrane with the molecular weight of 300 can remove inorganic salts in the peach gum polysaccharide solution.
The peach gum polysaccharide solution treated by the ultrafiltration membrane is basically changed from light yellow to colorless, and the effects of decoloring and impurity removal are synchronously realized; in addition, three different processes of desalting, deproteinizing and decoloring in the post-treatment are all completed through membrane filtering equipment, so that the post-treatment process is simplified, the use of an organic solvent is avoided, and the method is safer and more environment-friendly.
The invention also provides application of the peach gum polysaccharide prepared by the preparation method in preparation of products for promoting cell proliferation.
In the invention, the molecular weight of the peach gum polysaccharide is 0-100K, and more preferably 0-10K; the peach gum polysaccharide is present in the product in the form of a solution; in the peach gum polysaccharide solution, the concentration of the peach gum polysaccharide is 4-500 mug/ml, more preferably 4-100 mug/ml, and even more preferably 4-20 mug/ml.
In one embodiment of the invention, the molecular weight of the peach gum polysaccharide is 0-10K, and the concentration is 4-20 mug/ml.
In one embodiment of the invention, the peach gum polysaccharide has a molecular weight of 10-100K and a concentration of 4 μg/ml.
In the present invention, the cells are mammalian cells, more preferably one or more of human cells, murine cells and monkey cells, and still more preferably human fibroblasts.
According to the invention, the proliferation effect of the peach gum polysaccharide with smaller molecular weight is better, the effective concentration is lower, the cell proliferation effect shows a trend of increasing and then decreasing along with the increase of the concentration of the peach gum polysaccharide, the inflection point of the concentration of the peach gum polysaccharide is found to be 20 mug/ml, and more experimental data are provided for the application of the peach gum polysaccharide in cell proliferation.
The invention also provides application of the peach gum polysaccharide prepared by the preparation method in preparation of products for promoting cell anti-inflammatory.
In the invention, the molecular weight of the peach gum polysaccharide is 0-10K; the peach gum polysaccharide is in the form of a solution, and the concentration of the peach gum polysaccharide in the solution is 4-100 mug/ml.
In the present invention, the cells are mammalian cells, more preferably one or more of human cells, murine cells and monkey cells, and still more preferably Ana-1 cells.
The invention also provides cosmetics, which comprise the peach gum polysaccharide prepared by the preparation method and cosmetically acceptable auxiliary materials.
The cosmetic of the present invention is preferably a lotion, an emulsion, a foundation, an essence, a mask, a sunscreen, a face cream, an eye cream, a hand cream or a body cream; the mask is preferably a patch type mask or a smearing type mask; the essence is preferably essence water or essence oil.
The auxiliary materials are acceptable auxiliary materials in cosmetics, and are preferably one or more of solvents, thickeners, moisturizers, film forming agents, liposomes, preservatives, colorants, pH regulators and essence; the solvent is preferably water or an alcohol solvent.
In the invention, the molecular weight of the peach gum polysaccharide is 0-10K; the concentration of the peach gum polysaccharide is 4-100 mug/ml, and more preferably 4 mug/ml.
The invention applies amino acid to peach gum hydrolysis technology innovatively, ensures hydrolysis and avoids the use of strong alkali. Cell proliferation experiments prove that the proliferation effect of the peach gum polysaccharide prepared by the invention shows the characteristic of increasing and then decreasing along with the increase of the concentration of the peach gum polysaccharide, and the smaller the molecular weight is, the better the proliferation effect of the peach gum polysaccharide cells is; the cell anti-inflammatory experiment proves that the peach gum polysaccharide has a certain anti-inflammatory effect. The invention explores the correlation between the cell proliferation and anti-inflammatory effects and the molecular weight and concentration of the peach gum polysaccharide, and shows the application prospect of the peach gum polysaccharide in cosmetics.
The invention has the advantages that:
1. the safety is high: with the continuous development of the functional skin care product industry and the continuous increase of consumer demands on the functional, the functional skin care product is remarkably improved and increased. Correspondingly, product safety is becoming more and more of a consumer concern. However, the use of acid-base reagents and organic solvents is always unavoidable in the preparation process of products with a plurality of effects, but the residue of the reagents brings potential safety hazards, even causes serious problems of consumer allergy, redness and the like, so how to avoid the potential safety hazards from the source is the key point of research in recent years. The preparation process of the peach gum polysaccharide only adds basic amino acid which is a common raw material of cosmetics, does not introduce an organic solvent, strong acid, strong alkali and other allergic sources, can ensure the safety performance of the peach gum polysaccharide from the source, and further improves the safety performance of related skin care products.
2. Molecular weight is related to cell proliferation: in combination with the nature of peach gum polysaccharide itself, there is much research on moisture retention and oxidation resistance, but relatively little research on efficacy in terms of its cell proliferation, particularly the change between cell proliferation and peach gum molecular weight. The small molecular weight peach gum polysaccharide prepared by the invention not only shows remarkable effect of promoting fibroblast proliferation, but also shows the trend that the smaller the molecular weight of the peach gum polysaccharide is, the better the proliferation effect is. In addition, the proliferation effect tends to be increased and then reduced along with the increase of the concentration of the peach gum polysaccharide, and the application prospect in anti-aging cosmetics is shown.
3. The ratio of the small molecular polysaccharide is high: the molecular weight of polysaccharide is usually closely related to its biological activity, and macromolecular polysaccharide usually has moisturizing effect, while small molecular polysaccharide has various activities and effects due to the difference of sugar types. Therefore, in order to expand the efficacy and the application range of the peach gum polysaccharide, the higher the ratio of the small molecular polysaccharide should be, the better. According to the invention, when alkaline hydrolysis peach gum polysaccharide is used, a high-pressure homogenizer is selected for mechanical crushing, so that the molecular weight of the peach gum polysaccharide is further reduced, and the high ratio of small molecular polysaccharide is ensured. The results show that the polysaccharide ratio of the Dalton lower than 100K in the peach gum polysaccharide prepared by the method is more than 90%, and the polysaccharide ratio of the peach gum polysaccharide lower than 10K is more than 60%, which indicates that the method can effectively obtain a small molecular peach gum polysaccharide hydrolysate.
4. The process is simple and easy to produce: from the industrial production point of view, the simpler the production process, the more common the equipment is, the higher the industrialization degree is, and conversely, if the production process is complicated, the higher the equipment and raw material requirements are, the industrialization is difficult to realize. The preparation process of the peach gum extract is simple and easy to realize, all used instruments and equipment are common in industry, and the added raw materials are common raw materials in the field of cosmetics, so that the peach gum extract has the characteristics of simple process and easy production.
Drawings
FIG. 1 shows the effect of peach gum polysaccharide of different molecular weights and different concentrations on cell proliferation in experimental examples of the present invention;
FIG. 2 shows the anti-inflammatory effect of peach gum polysaccharides of different molecular weights and different concentrations in experimental examples of the present invention.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order to further illustrate the present invention, the following examples are provided. The raw materials used in the following examples of the present invention are all commercially available.
The main experiment and optimization contents of the preparation of the peach gum polysaccharide are shown in table 1.
TABLE 1 peach hydrolysis experiment Process parameter list
1. Examples
Example 1 optimization of swelling Experimental conditions
The raw peach gum with the same weight and cleaned impurities is taken to examine and reach the time of swelling balance under different swelling ratios and temperature conditions, and weighing is carried out every two hours, and the results are shown in table 2. Experimental results show that under the same temperature condition, the higher the solid-liquid ratio is, the shorter the peach gum reaches the swelling balance; under the same swelling ratio, the higher the temperature, the easier the swelling equilibrium is reached. This shows that an increase in the swelling ratio and an increase in the water temperature over a range are beneficial for the peach gum to reach swelling equilibrium quickly. By comparing the time under different experimental conditions, the temperature rise and the solid-liquid ratio increase, the reduction range of the swelling time is limited, the problems of water consumption, energy consumption and the like in actual production are considered, the influence of the water consumption, the energy consumption and the like on the time dimension is considered, and the peach gum swelling is preferably carried out under the conditions of the solid-liquid ratio of 1:20 and the water temperature and the normal temperature.
TABLE 2 statistical table of peach gum swelling experiment results
| Solid to liquid ratio | 1:10 | 1:15 | 1:20 | 1:30 |
| Normal temperature | Incomplete swelling | 28h | 24h | 20h |
| 45℃ | Incomplete swelling | 24h | 18h | 16h |
Example 2 hydrolysis experiment condition optimization
The aqueous solution with ph=10 was prepared with arginine, and peach gum powder (prepared by the optimal conditions in example 1) was added in different weights, hydrolyzed for different times in water at 50-60 ℃, and the peach gum hydrolysate was taken out to observe the hydrolysis state, respectively, and the results are shown in table 3. Along with the increase of the content of the peach gum, the viscosity of the peach gum hydrolysate is obviously increased, the color is gradually deepened, and the characteristic smell is also aggravated. However, with the increase of the peach gum content and the shortening of the hydrolysis time, a certain amount of granular peach gum appears in part of the hydrolysis liquid, which indicates that the peach gum is not completely hydrolyzed. In combination with the hydrolysis solution and the specific conditions during production, the peach gum content is preferably 1%, and the hydrolysis time is 12h as the optimal condition for hydrolysis.
TABLE 5 influence of peach gum content and hydrolysis time on hydrolysis effect
EXAMPLE 3 high pressure homogenization Condition exploration
The peach gum hydrolysate prepared under the optimal hydrolysis conditions in example 2 was subjected to high-pressure homogenization at treatment pressures of 100Bar, 300Bar and 500Bar, and the number of cycles was 3, and each set of experiments was repeated 3 times to perform parallel experiments, and the experimental results are shown in table 4. As can be seen from the table, as the homogenization pressure increases from 100Bar to 500Bar, the average particle size of the peach gum polysaccharide decreases from 1246.98nm to 523nm, exhibiting a tendency to decrease with increasing pressure, and the particle size reduction is more pronounced. The above results demonstrate that 500Bar can be selected as the working pressure for high pressure homogenization in order to obtain a smaller particle size peach gum polysaccharide solution.
TABLE 4 influence of high-pressure homogenizing pressure on peach gum particle size
EXAMPLE 4 Small molecule peach gum polysaccharide content
The invention uses a glycometer to test the total sugar content in stock solution (peach gum polysaccharide aqueous solution) and filtrate, and calculates the small molecular polysaccharide ratio by the following formula:
R＝K 2 /K 1 *100％
wherein R represents the content of small molecular weight peach gum polysaccharide; k (K) 1 、K 2 Respectively represents the total amount of sugar in the stock solution and the filtrate.
The peach gum polysaccharide aqueous solution prepared under the optimal conditions in example 3 is passed through a membrane separation device with molecular weights of 10K and 100K, the filtrate passing through the membrane separation device is the peach gum polysaccharide with molecular weight lower than that of the corresponding peach gum polysaccharide, then a nanofiltration membrane with molecular weight of 300 is used for removing inorganic salt in the peach gum polysaccharide solution, and finally a glycometer is used for testing the total content of polysaccharide in the filtrate, and the result is shown in Table 5. From the data in the table, the polysaccharide content of peach gum filtrate and hydrolysate at different concentrations has a very strong correlation with molecular weight, but the influence of concentration is relatively small. Wherein, the peach gum polysaccharide with the molecular weight of 10K accounts for about 60 percent of the total polysaccharide, and the peach gum polysaccharide with the molecular weight of 100K accounts for more than 90 percent of the total polysaccharide, and the results show that the polysaccharide content of the peach gum polysaccharide with the molecular weight lower than 100K in the prepared peach gum polysaccharide by the method is up to more than 90 percent, and the polysaccharide content of the peach gum polysaccharide with the molecular weight lower than 10K accounts for more than 60 percent, which indicates that the molecular weight of the peach gum polysaccharide is effectively reduced after hydrolysis and high-pressure homogenization.
TABLE 5 peach gum filtrate and stock solution total sugar ratio list
| Molecular weight of ultrafiltration membrane | Polysaccharide content of stock solution/% | Polysaccharide content of filtrate/% | Polysaccharide content in% by weight |
| 10K | 1 | 0.6 | 60 |
| 10K | 2.4 | 1.6 | 67 |
| 100K | 1 | 0.94 | 94 |
| 100K | 2.4 | 2.2 | 92 |
2. Experimental example
The peach gum polysaccharide in the experimental example is prepared under the optimal conditions in the above embodiment, and specifically comprises the following steps:
1.1 pretreatment of Primary peach gum
Mixing the raw peach gum with water according to the solid-to-liquid ratio of 1:20, soaking and expanding for 24 hours at normal temperature, removing impurities in the fully soaked peach gum, freeze-drying the peach gum, and finally crushing the dried peach gum and screening the crushed peach gum by a 100-mesh screen to obtain clean peach gum powder.
1.2 preparation of peach gum hydrolysate
Soaking the peach gum powder obtained in the step 1.1 with deionized water to obtain a solution with the pH value of about 10 (the content of the peach gum in the aqueous solution is 1 wt%) adjusted by using arginine, and carrying out hydrolysis experiments under certain conditions to obtain a peach gum solution, wherein the hydrolysis conditions are as follows: the pH value is 10, the hydrolysis temperature is 50-60 ℃, and the hydrolysis time is 12 hours. After the hydrolysis is completed, the pH of the hydrolysate is measured and is neutral.
1.3 high pressure homogenization of peach gum solution
And (3) homogenizing the peach gum hydrolysate prepared in the step (1.2) under high pressure, wherein the treatment pressure is 500Bar, and the cycle times are 3 times, so as to obtain the peach gum polysaccharide aqueous solution.
1.4 peach gum small molecular polysaccharide separation and post-treatment process
And (3) passing the peach gum polysaccharide aqueous solution prepared in the step (1.3) through membrane separation equipment with molecular weights of 10K and 100K, obtaining filtrate which is lower than the peach gum polysaccharide with the corresponding molecular weight through the membrane separation equipment, and removing inorganic salt in the peach gum polysaccharide solution by using a nanofiltration membrane with the molecular weight of 300.
1.5 lyophilization
In order to ensure the accuracy and the authenticity of the cell experiment, the separated peach gum polysaccharide with different molecular weights is freeze-dried, and PBS solutions with different concentrations are prepared by the weight of dry powder for the cell experiment.
2.1 cell proliferation experiments with peach gum polysaccharide
(1) Preparation of culture Medium and solution
(1) DMEM high sugar medium: adding a bag of DMEM high-sugar culture medium powder into 800mL of purified water, stirring for dissolving, and adding NaHCO 3 3.7g is continuously stirred until the powder is completely dissolved, the pH is regulated to 7.1-7.2, the volume is fixed to 1L, the powder is filtered and sterilized by a 0.22 mu m filter, and the powder is sealed and stored at 4 ℃.
(2) DMEM high sugar complete medium: FBS was added to DMEM high sugar medium to make the content 10%.
(3) MTT solution (5 mg. ML) -1 ): 50mg of MTT powder is dissolved in 10mL of 1 XPBS buffer solution under the condition of avoiding light, the solution is placed in a water bath at 60 ℃ to assist dissolution, a 0.22 mu m filter is used for filtration and sterilization, and the solution is placed at-20 ℃ to avoid light for storage.
(2) Cell proliferation assay
(1) Cell inoculation: the density of the human fibroblast suspension was adjusted to 8X 10 3 Inoculating into 96-well plate at a volume of 100 μl/well, placing at 37deg.C, 5% CO 2 Culturing in an incubator for 24 hours.
(2) Experimental grouping: negative control groups and sample groups were set, each group having 6 duplicate wells.
(3) Diluting and adding a sample to be tested: diluting the mother solution with complete culture medium as diluent, adding into cells at a certain concentration, 100 μl/well, adding the same amount of complete culture medium into negative control group, and standing at 37deg.C and 5% CO 2 Culturing in an incubator for 24 hours.
(4) MTT detection: under dark conditions, 10. Mu.L of MTT solution (5 mg. Multidot.mL) was added to each well -1 ) 96-well plates were placed at 37℃in 5% CO 2 Removing supernatant in the pore plate after fully reacting for 4 hours in the incubator, adding DMSO (dimethyl sulfoxide), 150 mu L/hole, shaking and dissolving for 10min, detecting absorbance value at 570nm of each hole by using a microplate reader, and taking 630nm as reference wavelength。
(5) And (3) calculating: after the background absorption is subtracted from each group of absorbance values, the cell viability percentage of the cells in each concentration sample addition group is calculated by taking the absorbance value of the cell control group as 100%, and the calculation formula is as follows:
percent cell viability (%) = sample well mean OD value/negative control mean OD value x 100%.
(6) Statistical analysis: data statistical analysis is performed by using GraphPadPrism8.0 software, a chart is drawn, metering data are expressed by x+/-s, inter-group differences are analyzed by using one-way ANOVA, and P < 0.05 is used as the difference, so that the statistical significance is achieved.
Cell OD values of each group were measured by MTT method and cell viability was calculated, and the results are shown in FIG. 1. The graph respectively verifies the influence of the change of the molecular weight and the change of the concentration of the peach gum polysaccharide on the cell proliferation effect, and the peach gum polysaccharide with the molecular weight lower than 10K has obvious cell proliferation effect, while the peach gum polysaccharide with the molecular weight between 10K and 100K only has the cell proliferation effect at the concentration of 4 mug/ml, and the cell proliferation effect of the peach gum polysaccharide with the molecular weight higher than 100K is basically not obvious, so that the cell proliferation effect of the peach gum polysaccharide has close relation with the molecular weight, and the peach gum polysaccharide with the molecular weight lower than 10K can obviously promote the cell proliferation.
And then analyzing the cell proliferation effect of the peach gum polysaccharide with different concentrations of less than 10K, the effect of cell proliferation shows a trend of increasing and then decreasing along with the increase of the concentration of the peach gum, and the optimal concentration of the cell proliferation is 20 mug/ml.
The results show that the cell proliferation effect of the peach gum polysaccharide is obviously related to the molecular weight of the peach gum polysaccharide, the peach gum polysaccharide with the cell proliferation effect of less than 10K is obviously related to the cell proliferation effect, and the proliferation effect of the peach gum polysaccharide is increased and then reduced along with the increase of the concentration of the peach gum polysaccharide.
2.2 cell anti-inflammatory experiments with peach gum polysaccharide
(1) Preparation of culture Medium and solution
(1) RPMI 1640 complete medium: FBS was added to RPMI 1640 medium to a 10% level.
(2) LPS: a1 mg/ml LPS solution was prepared using 1 XPBS buffer as the diluent.
(2) Cell anti-inflammatory assay
(1) Cell inoculation: ana-1 cells were seeded at 4000 cells/well in 96-well plates at 37℃in 5% CO 2 Culturing in an incubator for 24 hours.
(2) LPS induces an inflammatory response: diluting 1mg/ml LPS solution to 3 μg/ml, adding the same amount of culture medium into a 50 μl/Kong Jiazhi well plate, placing at 37deg.C, and 5% CO 2 Culturing in an incubator for 4 hours.
(3) Diluting and adding a sample to be tested: diluting the sample with complete medium as diluent to give working concentration of 100 μl/well, adding the same amount of complete medium to the negative control group, and placing at 37deg.C, 5% CO 2 Culturing in an incubator for 24 hours.
(4) The IL-6 content was detected by ELISA, and the procedure was as follows:
A. and (5) precalculating the required ELISA strips, taking out the kit 30min before the experiment, and recovering to room temperature.
B. Standard gradient dilution: the standard was diluted to 500, 250, 125, 62.5, 31.25,0pg/mL with standard & sample dilutions.
C. Cell culture supernatants were collected in sterile centrifuge tubes, centrifuged (4 ℃,1000×g,20 min), and the supernatants were diluted 20-fold to obtain test samples.
D. 100 mu L of standard working solution and detection samples are added into each reaction hole, 2 compound holes are arranged in each group, and the mixture is incubated for 90min at 37 ℃.
E. Removing the liquid, spin-drying, adding 100 mu L of biotin-labeled interleukin 6 antibody working solution into each reaction well, and incubating in an incubator at 37 ℃ for 60min.
F. Removing liquid, spin-drying, adding 300 μl of washing liquid into each reaction well, soaking for 1-2min, and spin-drying. Repeated 4 times.
G. 100 mu L of HRP-labeled streptavidin working solution is added into each reaction well, and the mixture is incubated for 30min at 37 ℃ in an incubator.
H. 300 mu L of washing liquid is added into each reaction hole, the washing liquid is dried at intervals of 30 seconds, and the washing liquid is repeated for 4 times.
I. 90 mu L of color reagent is added into each reaction hole, and color development is carried out at 37 ℃ for about 15min in a dark place.
J. 50 mu L of stop solution was added to each reaction well, and the OD value was measured immediately at a wavelength of 450nm by using an ELISA reader.
K. From the known concentration of the standard and the measured OD value, a standard curve regression equation (R 2 >0.99 Substituting the OD value of the sample hole into the concentration of the sample to be measured, and multiplying the concentration by the dilution multiple to obtain the actual IL-6 concentration of the original sample.
(5) Statistical analysis: data statistical analysis is carried out by adopting GraphPad prism8.0 software, a chart is drawn, metering data is expressed by x+/-s, inter-group differences are analyzed by adopting one-way ANOVA, and P < 0.05 is taken as the difference, so that the statistical significance is achieved.
The OD values of the cells of each group were measured by ELISA and anti-inflammatory effect was calculated, and the results are shown in fig. 2. The experiment verifies the anti-inflammatory effect of peach gum polysaccharide with different molecular weights, and the result shows that the peach gum polysaccharide with the molecular weight smaller than 10K has remarkable anti-inflammatory effect, while the peach gum polysaccharide with the molecular weight larger than 10K cannot show anti-inflammatory effect. The results show that the anti-inflammatory effect of the peach gum polysaccharide is related to the molecular weight, and the peach gum polysaccharide with the molecular weight lower than 10K has a certain anti-inflammatory effect, so that the potential application value of the peach gum polysaccharide in cosmetics is reflected.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. The preparation method of the peach gum polysaccharide is characterized by comprising the following steps: mixing peach gum with amino acid, and hydrolyzing to obtain peach gum polysaccharide;
homogenizing the hydrolyzed peach gum polysaccharide under high pressure;
separating and post-treating peach gum polysaccharide after high-pressure homogenization;
the amino acid is one or more of lysine, arginine and histidine;
mixing the peach gum with the amino acid solution, wherein the mass ratio of the peach gum to the amino acid solution is (0.5-2) 100;
the hydrolysis time is 6-24 hours;
the hydrolysis temperature is 50-60 ℃;
the pH value of the hydrolysis is 8-10;
the pressure of the high-pressure homogenization is 100-500 Bar;
the separation adopts membrane separation equipment with molecular weights of 10K and 100K, and peach gum polysaccharide with molecular weights below 10K and 10K-100K are collected;
no strong base is introduced in the preparation method.
2. The use of peach gum polysaccharide prepared by the preparation method of peach gum polysaccharide according to claim 1 in the preparation of products for promoting proliferation of human fibroblasts;
the peach gum polysaccharide is present in the product in the form of a solution; in the peach gum polysaccharide solution, the molecular weight of the peach gum polysaccharide is below 10K, and the concentration of the peach gum polysaccharide is 4-500 mug/ml; the molecular weight of the peach gum polysaccharide is 10K-100K, and the concentration of the peach gum polysaccharide is 4 mug/ml.
3. The use according to claim 2, characterized in that the peach gum polysaccharide has a molecular weight of 0-10 k; in the peach gum polysaccharide solution, the concentration of the peach gum polysaccharide is 4-20 mug/ml.
4. The use of peach gum polysaccharide prepared by the preparation method of peach gum polysaccharide according to claim 1 for preparing a product promoting cell anti-inflammatory;
the molecular weight of the peach gum polysaccharide is 0-10K; in the peach gum polysaccharide solution, the concentration of the peach gum polysaccharide is 4-100 mug/ml.
5. Cosmetic, characterized by comprising the peach gum polysaccharide prepared by the preparation method of the peach gum polysaccharide of claim 1 and cosmetically acceptable auxiliary materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211190678.1A CN115368484B (en) | 2022-09-28 | 2022-09-28 | Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211190678.1A CN115368484B (en) | 2022-09-28 | 2022-09-28 | Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115368484A CN115368484A (en) | 2022-11-22 |
| CN115368484B true CN115368484B (en) | 2023-11-24 |
Family
ID=84073522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211190678.1A Active CN115368484B (en) | 2022-09-28 | 2022-09-28 | Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115368484B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116549328A (en) * | 2023-06-16 | 2023-08-08 | 广州思研生物技术有限公司 | Use of peach gum polysaccharide in products for promoting tissue repair or improving aesthetic skin |
| CN116693713B (en) * | 2023-06-16 | 2024-04-26 | 科乐美(广州)生物科技有限公司 | Peach gum polysaccharide and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013065705A1 (en) * | 2011-10-31 | 2013-05-10 | 興和株式会社 | Powdery cosmetic |
| KR102130149B1 (en) * | 2019-12-11 | 2020-07-03 | (주)더마랩 | Cosmetic composition containing peach gum extract |
| CN111454374A (en) * | 2020-04-29 | 2020-07-28 | 上海珈凯生物科技有限公司 | Peach gum extract with skin tightening effect, and preparation method and application thereof |
| WO2021045504A1 (en) * | 2019-09-03 | 2021-03-11 | 주식회사 엘지생활건강 | Cosmetic material composition comprising solid phase polymer having improved solubility |
-
2022
- 2022-09-28 CN CN202211190678.1A patent/CN115368484B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013065705A1 (en) * | 2011-10-31 | 2013-05-10 | 興和株式会社 | Powdery cosmetic |
| WO2021045504A1 (en) * | 2019-09-03 | 2021-03-11 | 주식회사 엘지생활건강 | Cosmetic material composition comprising solid phase polymer having improved solubility |
| KR102130149B1 (en) * | 2019-12-11 | 2020-07-03 | (주)더마랩 | Cosmetic composition containing peach gum extract |
| CN111454374A (en) * | 2020-04-29 | 2020-07-28 | 上海珈凯生物科技有限公司 | Peach gum extract with skin tightening effect, and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 向燕茹等."原桃胶粗多糖单糖组成分析及其活性研究".《食品科技》.2019,(第05期),第199-203、207页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115368484A (en) | 2022-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115368484B (en) | Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics | |
| Huang et al. | Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus | |
| CN111454844B (en) | Novel ganoderma lucidum strain, ganoderma lucidum polysaccharide prepared based on ganoderma lucidum strain and anti-aging cosmetic | |
| CN113337545B (en) | Schizophyllum commune fermentation product, preparation method thereof, skin care product and schizophyllum commune culture medium | |
| CN108047343B (en) | Preparation method and application of fritillaria pallidiflora total polysaccharide | |
| CN107674129A (en) | Schizophan phosphorylated derivative and preparation method thereof, application | |
| CN102746410A (en) | Method for preparing high-content fenugreek gum | |
| CN112545962A (en) | Moisturizing and bacteriostatic gel containing aloe arborescens extract and preparation method thereof | |
| WO2015071477A1 (en) | Method for obtaining extracts of marine algae | |
| CN112353800B (en) | Composition of hyaluronic acid or salt thereof and/or trehalose and use thereof in stabilizing ergothioneine | |
| CN114886800B (en) | Application of black tea polysaccharide | |
| CN105533120B (en) | One preparation method for cultivating peanut nano peptide | |
| CN109276588B (en) | Application of wife extract in preparing composition for inhibiting MMP2 gene expression | |
| CN107669525B (en) | Application of Poria extract with whitening effect and its preparation method | |
| CN113952264B (en) | Application of lycium ruthenicum extract in preparation of skin care product and skin care product | |
| Sed et al. | Extraction and purification of exopolysaccharides from exhausted Arthrospira platensis (Spirulina) culture systems | |
| CN113041200A (en) | Cosmetic containing multiple fermentation liquor of custard apple and preparation method thereof | |
| CN113813209A (en) | Rose petal fermentation liquor and preparation method and application thereof | |
| TW201739459A (en) | Polysaccharide extract of microalgae and extraction method thereof | |
| CN111407702A (en) | Skin-care face cream containing sophora flower and seaweed and preparation method of skin-care face cream | |
| CN115746166B (en) | Fructus trichosanthis She Guojiao and preparation method and application thereof | |
| CN115715747B (en) | Application of black tea polysaccharide in preparation of compression-resistant product | |
| CN115975066B (en) | Production method and application of food-grade tremella polysaccharide | |
| CN113559014B (en) | Use of carboxymethyl chitosan for promoting fibroblast proliferation and increasing collagen content | |
| CN115068387B (en) | Plant composition and application thereof in cosmetics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |