CN115367255A - Reagent kit - Google Patents

Abstract

A kit comprises a box body, a top cover body and a side cover body. The box body includes the diapire and a plurality of lateral walls that set up around the edge of diapire, and lateral wall and diapire enclose jointly and establish and form the accommodation space, and the accommodation space includes a plurality of reagent grooves that are used for loading reagent. One side wall is provided with a plurality of flow holes, each flow hole is communicated with a reagent groove, and the outer surface of the side wall with the flow holes is also convexly provided with a plurality of flow passage plates. The top cap body is arranged at one end of the side walls far away from the bottom wall, is used for closing the accommodating space and is provided with a plurality of first liquid suction ports. The side cover body is arranged on one side, far away from the side wall, of the runner plate, each runner plate, the side wall and the side cover body are arranged in a surrounding mode to form a runner, and the runners are communicated with the circulation holes and the first liquid suction ports and communicated with the corresponding reagent grooves through the circulation holes. A sealed state can be formed in the flow passage. The application avoids the situation that the reagent in the reagent tank can be maximally utilized only by inserting the reagent needle into the bottom of the reagent tank.

Description

Reagent kit

Technical Field

The application relates to the field of biochemical detection, in particular to a kit.

Background

In the field of biochemistry, a plurality of different reagents are generally used for detecting biological samples, and operators need to prepare various reagents, reagent tubes and other auxiliary tools in advance before detection can be carried out. The reagent kit is provided with a plurality of independent reagent grooves, various required reagents can be intensively placed, and an operator can absorb the required reagents only by inserting the reagent needles into the corresponding reagent grooves, so that the detection is convenient.

However, the reagent needle needs to be inserted into the bottom of the reagent tank to maximally utilize the reagent in the reagent tank, and thus, when the reagent tank is deep, the length of the reagent needle needs to be increased accordingly, and a large stroke of the reagent needle insertion may result. Moreover, after the reagent needle is inserted into the bottom of the reagent tank, the vast area of the outer wall of the reagent needle can be soaked in the reagent, and before the reagent of a different type needs to be sucked, the reagent attached to the outside of the reagent needle needs to be completely cleaned, so that the use amount of cleaning liquid and the cleaning difficulty are increased to a certain extent.

Disclosure of Invention

In order to solve the above disadvantages of the prior art, it is necessary to provide a kit.

The application provides a kit, including box body, top lid body and side lid body. The box body includes the diapire and centers on a plurality of lateral walls that the edge of diapire set up, the lateral wall with the diapire encloses jointly and establishes and forms the accommodation space, the accommodation space includes a plurality of reagent grooves that are used for loading reagent. The side wall is provided with a plurality of circulation holes, each circulation hole is communicated with one reagent groove, and the outer surface of the side wall with the circulation holes is also convexly provided with a plurality of runner plates. The top cover body is arranged at one end, far away from the bottom wall, of the side wall, and used for closing the accommodating space, and a plurality of first liquid suction ports are formed in the top cover body. The side cover body is arranged on one side, far away from the side wall, of the runner plate, each runner plate, the side wall and the side cover body are jointly surrounded to form a runner, and the runners are communicated with the circulation holes and the first liquid suction ports and communicated with the corresponding reagent grooves through the circulation holes. A sealed state can be formed in the flow passage.

In some possible implementation manners, the box body further includes an extension plate, the extension plate is convexly disposed on the outer surface of the side wall having the circulation hole, and is disposed between the top cover body and the runner plate, each runner plate is connected to the extension plate, a plurality of second liquid suction ports are disposed on the extension plate, and the second liquid suction ports are communicated between the first liquid suction ports and the runner. And a first sealing element is arranged between the top cover body and the extension plate, a plurality of protruding parts are arranged on the first sealing element, the protruding parts extend into the second liquid suction port, and the protruding parts are used for enabling the flow channel to form a sealing state.

In some possible implementations, the case further includes an extension plate,

the side cover body comprises a first cover body part and a second cover body part connected with the first cover body part, the first cover body part is arranged on one side, away from the side wall, of the runner plate, and the second cover body part is arranged between the top cover body and the extension plate. The second cover body part is provided with a plurality of third liquid suction ports, and each third liquid suction port is communicated between the first liquid suction port and the second liquid suction port.

In some possible implementations, each of the flow channel plates includes a bottom portion and side portions connected to two sides of the bottom portion, the bottom portion is disposed near the bottom wall, the side portions are connected to the extension plate, and the bottom portion, the two side portions, the extension plate, the side walls, and the side cover body of each of the flow channel plates jointly enclose the flow channel.

In some possible implementations, a second sealing member is disposed between the runner plate and the side cover body, and the second sealing member is used for forming a sealing state in the runner.

In some possible implementations, the accommodating space includes a first area, and a plurality of first partition plates are disposed in the first area, and the first partition plates are used to divide the first area into the reagent tanks.

In some possible implementations, the accommodating space further includes a second area, and a plurality of second partitions are disposed in the second area, and the second partitions are used for dividing the second area into a plurality of premix tanks for pre-storing the reagents. Each premixing groove is internally provided with a premixing bottle for pre-storing another reagent, and the premixing bottle is internally provided with a puncturing piece which is used for puncturing the bottom of the premixing bottle when being pressed.

In some possible implementations, a third seal is disposed between the inner wall of the premix bottle and the spike for sealing a gap between the inner wall of the premix bottle and the spike.

In some possible implementations, a pressing portion is disposed on the top cap body, the pressing portion is disposed corresponding to the puncturing element, and the pressing portion is configured to push the puncturing element after being pressed.

In some possible implementation manners, a blocking piece is arranged on the top cover body, the blocking piece is arranged between the pressing part and the puncturing part, a sliding groove is further formed in the top cover body, and part of the blocking piece is exposed out of the sliding groove and can slide in the sliding groove. The separation blade can be switched between a first state and a second state when sliding, the first state is that the separation blade does not shield the puncture piece, and the second state is that the separation blade shields the puncture piece.

In contrast to the prior art, in the present application, when a reagent needs to be aspirated, a reagent needle may be inserted (or tubing connected) into the first aspiration port. Because the flow channel can be in a sealed state, along with the suction action of the reagent needle (or the pipeline), the reagent in the reagent groove can be driven to flow into the flow channel through the circulation hole and further sucked by the reagent needle (or the pipeline), and the condition that the reagent in the reagent groove can be maximally utilized only by inserting the reagent needle into the bottom of the reagent groove is avoided. Therefore, this application is favorable to reducing the washing degree of difficulty and the washing liquid quantity that washs used of reagent needle, also is favorable to making reagent needle more miniaturized.

Drawings

Fig. 1 is a schematic structural diagram of a kit provided in an embodiment of the present application.

Fig. 2 is an exploded view of the kit shown in fig. 1.

FIG. 3 is a sectional view of the kit shown in FIG. 1 taken along line III-III.

Fig. 4 is a sectional view along IV-IV of the kit shown in fig. 1.

Description of the main elements

Box body 10

Bottom wall 11

Side wall 12

First partition plate 13

The runner plate 14

Extension plate 15

Second partition plate 16

Premix bottle 17

Piercing member 18

Top cover body 20

Filling opening 21

First liquid suction port 22

Pressing part 23

Baffle plate 24

Side cover 30

First lid part 31

Second lid portion 32

Second seal 40

First seal 50

Projection 51

Third seal 60

Kit 100

Flow holes 120

First side 131

Second side 132

Flow passage 140

Bottom 141

Side 143

Second liquid suction port 150

Chute 200

Connecting end 231

Free end 232

Third liquid suction port 320

Accommodating space 1000

First region 1001

Second region 1002

Reagent tank 1003

Premix tank 1004

The following detailed description will further illustrate the present application in conjunction with the above-described figures.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments.

It is noted that when an element is referred to as being "on" another element, it can be directly on the other element or intervening elements may also be present; when a component is referred to as being "mounted on" another component, it can be directly mounted on the other component or intervening components may also be present. As used herein, the term "and/or" includes all and any combination of one or more of the associated listed items.

Referring to fig. 1 and 2, one embodiment of the present application provides a kit 100 for loading a plurality of reagents required for a biochemical test process. The kit 100 includes a case 10, a top cover 20, and a side cover 30.

The case 10 includes a bottom wall 11 (shown in fig. 3), a plurality of side walls 12, and a plurality of first partitions 13. The side wall 12 is disposed around a plurality of edges of the bottom wall 11, such that the side wall 12 and the bottom wall 11 jointly enclose the accommodating space 1000. The accommodating space 1000 is divided into a first region 1001 and a second region 1002.

The plurality of first partitions 13 are provided in the first region 1001 of the accommodating space 1000, and divide the first region 1001 into a plurality of reagent tanks 1003 that are independent of each other. Each reagent well 1003 is for holding one reagent. In some embodiments, the volumes of the plurality of reagent tanks 1003 are different from each other, such that the plurality of reagent tanks 1003 may be used to store different volumes of reagents, respectively. Therefore, in actual use, a reagent may be loaded into the corresponding reagent well 1003 according to the amount of the reagent required in the biochemical process. In some embodiments, each first baffle 13 can be non-planar or planar, and each first baffle 13 includes opposing first and second sides 131, 132. The first sides 131 of the first partitions 13 are connected to one of the sidewalls 12, and the second sides 132 of the first partitions 13 are connected to the other sidewalls 12, so that the first partitions 13 are radially distributed from one of the sidewalls 12.

The top cover 20 is disposed at an end of the side walls 12 away from the bottom wall 11. The top cover body 20 is used for closing the accommodating space 1000, so that the risk of reagent overflowing from the reagent tank 1003 is reduced, and a protection effect is also achieved. In some embodiments, the top cover 20 is provided with a plurality of filling openings 21, and each filling opening 21 corresponds to one reagent tank 1003. The user can add a reagent into the corresponding reagent tank 1003 through the liquid addition port 21, and can let atmospheric air flow into the reagent tank 1003 when in use. In general, a sealing film (not shown) is disposed at an opening of the accommodating space 1000, and the sealing film needs to be punctured when adding a reagent into the corresponding reagent tank 1003 or allowing the air to flow into the reagent tank 1003.

Referring to fig. 2 and 3, at least one of the side walls 12 is provided with a plurality of flow holes 120 arranged at intervals, and each flow hole 120 is communicated with one reagent tank 1003. The outer surface of the sidewall 12 having the flow holes 120 is also embossed with a plurality of flow field plates 14. The side cover 30 is provided on the side of the flow field plate 14 remote from the side wall 12. Each flow channel plate 14, the side wall 12 and the side cover 30 together define a flow channel 140. The top cover 20 has a plurality of first liquid suction ports 22, and the flow channel 140 communicates with a flow hole 120 and a first liquid suction port 22. The volume of the flow channel 140 may be smaller than the volume of the corresponding reagent reservoir 1003, and a sealed state can be formed in the flow channel 140. In some embodiments, the flow openings 120 may be disposed adjacent the bottom wall 11.

Therefore, when it is necessary to aspirate a reagent, a reagent needle (not shown) can be inserted into the first aspirating port 22. Because the flow channel 140 is in a sealed state, the reagent in the reagent tank 1003 can be driven to flow into the flow channel 140 through the flow hole 120 along with the sucking action of the reagent needle, and then the reagent needle is sucked, so that the situation that the reagent in the reagent tank 1003 can be maximally utilized only by inserting the reagent needle into the bottom of the reagent tank 1003 is avoided. Consequently, this application is favorable to reducing the washing degree of difficulty and the washing liquid quantity that washs used of reagent needle, also is favorable to making the reagent needle more miniaturized. In the invention, the reagent needle can be made very short or even not designed, as long as a pipeline capable of being connected with the liquid suction port is designed.

In some embodiments, the outer surface of the sidewall 12 having the flow holes 120 is also embossed with an extension plate 15. The extension plate 15 is provided between the top cover body 20 and the runner plates 14, and each runner plate 14 is connected to the extension plate 15. The extension plate 15 is provided with a plurality of second liquid suction ports 150 arranged at intervals. The second liquid suction port 150 communicates between the first liquid suction port 22 and the flow channel 140. In some embodiments, each flow field plate 14 includes a bottom portion 141 and side portions 143 connected to both sides of the bottom portion 141. The bottom 141 may be disposed adjacent the bottom wall 11 and the side 143 connects the extension panel 15. The bottom 141, the two sides 143, the extension plate 15, the sidewall 12 and the side cover 30 of each flow channel plate 14 collectively enclose the flow channel 140.

A first sealing member 50 is further provided between the top cover body 20 and the extension plate 15. The first sealing member 50 is provided with a plurality of projections 51 through which reagent needles (or tubes) can pass, and the projections 51 are projected into the second pipette port 150. The protrusion 51 is used to seal the gap between the second liquid suction port 150 and the reagent needle (or tube) when the reagent needle (or tube) is inserted into the first liquid suction port 22 and the second liquid suction port 150 in sequence, so that a sealed state is formed in the flow channel 140, and the reagent in the flow channel 140 can be conveniently sucked.

In some embodiments, the side cover 30 includes a first cover portion 31 and a second cover portion 32 connected to the first cover portion 31. The first cover portion 31 is provided on a side of the flow field plate 14 away from the side wall 12. The second lid portion 32 may be substantially perpendicular to the first lid portion 31, with the second lid portion 32 disposed between the top lid 20 and the first seal 50. The second cap portion 32 has a plurality of third liquid-absorbing ports 320, and each of the third liquid-absorbing ports 320 is connected between the first liquid-absorbing port 22 and the second liquid-absorbing port 150. At this time, the reagent needle (or tube) may be inserted into the first, third, and second pipette ports 22, 320, and 150 in order to aspirate the reagent in the flow channel 140.

In some embodiments, a second sealing member 40 is disposed between the flow channel plate 14 and the side cover 30, and the second sealing member 40 is used to seal a gap between the edge of the flow channel plate 14 away from the side wall 12 and the side cover 30, so as to maintain a sealed state in the flow channel 140 and ensure that reagents in different flow channels 140 are not mixed with each other.

Referring to fig. 2 and 4, in some embodiments, a plurality of second partitions 16 are disposed in the second region 1002, and the second partitions 16 are used to divide the second region 1002 into a plurality of pre-mixing slots 1004, and generally, the pre-mixing slots 1004 pre-store reagents. At least one premixing bottle 17 is arranged in each premixing groove 1004, and another reagent is pre-stored in the premixing bottle 17. Also disposed within each premix bottle 17 is a spike 18. The piercer 18 is used to pierce the bottom of the premix bottle 17 when pressed, so that the pre-stored reagent in the premix bottle 17 flows out to the premix tank 1004, thereby mixing the two reagents. In some embodiments, the bottom of the premix bottle 17 may be a readily pierceable membrane layer. The piercing member 18 may be a piercing plunger.

Further, a third seal 60 may be disposed between the inner wall of the premix bottle 17 and the spike 18. The third seal 60 serves to seal the gap between the inner wall of the premix bottle 17 and the puncturer 18, thereby reducing the risk of the reagent within the premix bottle 17 escaping upwardly from between the inner wall of the premix bottle 17 and the puncturer 18. The third seal 60 may be an O-ring seal.

Further, as shown in fig. 1 and 2, the top cap 20 may be provided with at least one pressing portion 23, and each pressing portion 23 is disposed corresponding to the puncturing member 18 in one of the premix bottles 17. The pressing part 23 includes a connection end 231 connected to the top cover body 20 and a free end 232 opposite to the connection end 231. When the user presses on this press 23, the free end 232 of the press 23 moves toward the spike 18, thereby pushing the spike 18 to pierce the bottom of the premix bottle 17.

Furthermore, at least one blocking piece 24 is further disposed on the top cap body 20, and each blocking piece 24 is disposed between one of the pressing portions 23 and the corresponding puncturing element 18. The flap 24 is switchable between a first state and a second state when operated by a user. The first state is when the stopper 24 does not cover the puncturing member 18, so that the pressing portion 23 can push the puncturing member 18 to puncture the bottom of the premix bottle 17 after being pressed. In the second state, the stopper 24 shields the plunger 18, so that the pressing portion 23 is blocked by the stopper 24 after being pressed, and the plunger 18 cannot be pushed to pierce the bottom of the premix bottle 17. Thus, when no pre-mix reagents are required, the flap 24 is operable to switch to the second state, thereby reducing the risk of the spike 18 being accidentally depressed and piercing the bottom of the pre-mix vial 17.

The top cover 20 may have a sliding slot 200, and the blocking sheet 24 may be partially exposed from the sliding slot 200 for user operation. For example, when the user pushes a portion of the flap 24 exposed to the chute 200 to slide back and forth in the chute 200, the flap 24 can be switched between the first state and the second state.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present application and not for limiting, and although the present application is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present application without departing from the spirit and scope of the technical solutions of the present application.

Claims (10)

1. A kit, comprising:

the reagent box comprises a box body and a plurality of reagent containers, wherein the box body comprises a bottom wall and a plurality of side walls arranged around the edge of the bottom wall, the side walls and the bottom wall jointly enclose to form an accommodating space, and the accommodating space comprises a plurality of reagent grooves for loading reagents; the side wall is provided with a plurality of flow holes, each flow hole is communicated with one reagent groove, and the outer surface of the side wall with the flow holes is also convexly provided with a plurality of flow passage plates;

the top cover body is arranged at one end of the side walls far away from the bottom wall, is used for closing the accommodating space and is provided with a plurality of first liquid suction ports; and

the side lid is located the runner plate is kept away from one side of lateral wall, each the runner plate the lateral wall with the side lid encloses jointly and establishes and form a runner, the runner intercommunication the opening with first imbibition mouth, and pass through the opening intercommunication corresponds the reagent groove, can form encapsulated situation in the runner.

2. The kit of claim 1, wherein the case further comprises an extension plate protruding from an outer surface of the sidewall having the flow hole and disposed between the top cover and the flow channel plate, each of the flow channel plates is connected to the extension plate, the extension plate is provided with a plurality of second liquid-suction ports, and the second liquid-suction ports are connected between the first liquid-suction ports and the flow channel;

and a first sealing element is arranged between the top cover body and the extension plate, a plurality of protruding parts are arranged on the first sealing element, the protruding parts extend into the second liquid suction port, and the protruding parts are used for enabling the flow channel to form a sealing state.

3. The kit of claim 2, wherein the side cover includes a first cover portion disposed on a side of the flow field plate remote from the side wall and a second cover portion coupled to the first cover portion, the second cover portion disposed between the top cover and the extension plate;

the second cover body part is provided with a plurality of third liquid suction ports, and each third liquid suction port is communicated between the first liquid suction port and the second liquid suction port.

4. The reagent kit of claim 2, wherein each of said flow field plates comprises a bottom portion and side portions connected to both sides of said bottom portion, said bottom portion is disposed adjacent to said bottom wall, said side portions are connected to said extension plate, and said bottom portion, two of said side portions, said extension plate, said side walls and said side cover body of each of said flow field plates jointly enclose said flow field.

5. The reagent cartridge according to claim 1, wherein a second sealing member for forming a sealed state in the flow path is provided between the flow path plate and the side cover body.

6. The reagent kit of claim 1, wherein the accommodating space comprises a first area, and a plurality of first partition plates are arranged in the first area and are used for dividing the first area into the reagent tanks.

7. The reagent kit of claim 6, wherein the accommodating space further comprises a second area, a plurality of second partition plates are arranged in the second area, and the second partition plates are used for dividing the second area into a plurality of premixing grooves for pre-storing reagents;

each premixing groove is internally provided with a premixing bottle for pre-storing another reagent, and the premixing bottle is internally provided with a puncturing piece which is used for puncturing the bottom of the premixing bottle when being pressed.

8. The kit of claim 7, wherein a third seal is disposed between the interior wall of the premix bottle and the spike and seals a gap between the interior wall of the premix bottle and the spike.

9. The reagent kit of claim 7, wherein the top cover body is provided with a pressing portion, the pressing portion is arranged corresponding to the puncturing member, and the pressing portion is used for pushing the puncturing member after being pressed.

10. The reagent kit of claim 9, wherein the top cover body is provided with a blocking piece, the blocking piece is arranged between the pressing part and the puncture piece, the top cover body is further provided with a sliding groove, and the blocking piece is partially exposed out of the sliding groove and can slide in the sliding groove;

the separation blade can switch between first state and second state when sliding, the first state is that the separation blade does not shelter from the piercing member, the second state is that the separation blade shelters from the piercing member.

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