CN1153635A - Prepn of bezoar - Google Patents
Prepn of bezoar Download PDFInfo
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- CN1153635A CN1153635A CN95119587A CN95119587A CN1153635A CN 1153635 A CN1153635 A CN 1153635A CN 95119587 A CN95119587 A CN 95119587A CN 95119587 A CN95119587 A CN 95119587A CN 1153635 A CN1153635 A CN 1153635A
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- bilirubin
- salt
- calculus bovis
- aggretion type
- room temperature
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Abstract
The present invention first produces polymer bilirubin through bilirubin polymerase-H enzyme process, then produces macromolecular polymer bilirubin salts through the combination of bilirubin with calcium salt or alkali, and finally produces artificial bezoar through the compounding with other components. The artificial bezoar product is same as natural bezoar in main chemical components, structure and physical and chemical properties, and it may be used to replace natural bezoar in pharmaceutical industry.
Description
The present invention relates in the main chemical compositions of natural Calculus Bovis and the manufacture method of ratio manufacture of intraocular Calculus Bovis thereof, the manufacture method of especially new Calculus Bovis belongs to made medicinal preparation.
Natural Calculus Bovis is a kind of rare Chinese medicine, is the calculus that is grown in the mammal cattle gallbladder, presses the document record, and its main chemical compositions and content are as follows:
Total bilirubin 12.9-55.8%
Total bile acid 7.43-10.0%
Taurine 0.66-1.07%
Cholesterol 2.5-4.3%
Amino acid/11 5.5-17.6%
Unconjugated bilirubin 0.6-1.0%
Trace element 2.3-2.6%
Lecithin 1.0-2.0%
The total bilirubin content of natural Calculus Bovis is up to more than 65%, and unconjugated bilirubin is the highest to be no more than 1.0%, and its existence form is to exist with a kind of combining form.
The artificial Calculus Bovis that China uses at present prolongs with unified the prescription and standard in the health whole nation promulgated by the ministries or commissions of the Central Government in 1971.It is reported Shanghai Chinese medicine one factory on the basis of former artificial Calculus Bovis, use the starch in the former artificial Calculus Bovis instead animal bile dry powder and form artificial Calculus Bovis's new prescription, obtain the Ministry of Public Health authentication code and put into production April nineteen ninety-five as charges.This product is the succedaneum of former artificial Calculus Bovis, but its total bilirubin content is no more than 1.0%, and is unconjugated bilirubin, thereby can not replace natural Calculus Bovis to be used as medicine.
The manufacture method that the purpose of this invention is to provide a kind of new Calculus Bovis adopts the enzyme process of bilirubin polymerase-H to make the polymerizable molecular bilirubin earlier, is combined into macromole aggretion type bilirubin salt with salt such as calcium or its alkali again, is equipped with other compositions afterwards and makes new Calculus Bovis.
The new Calculus Bovis product that adopts this method to make; configuration state and physicochemical property with main chemical compositions of natural Calculus Bovis; meet the detection regulation of Pharmacopoeia of People's Republic of China (version in 1992), in pharmaceuticals industry, can replace natural Calculus Bovis the natural Calculus Bovis physicochemical property.
The manufacture method of the new Calculus Bovis of the present invention is as follows:
1, the preparation method of aggretion type bilirubin salt
(1) uses the bilirubin powder, preparation method thereof
Bilirubin added in rare alkali ethanol water make its dissolving, transfer PH to neutral with diluted acid, add a certain amount of bilirubin polymerase-H, effect is about 30 minutes under the room temperature, adding calcium salt (or magnesium, zinc salt) more fully stirs, then repeatedly behind the washing filtering,, promptly get aggretion type calcium bilirubinate (or magnesium, zinc salt) with filtrate vacuum drying at room temperature.
(2) with the direct synthetic method of bile
With the bright bile of cattle, pig or sheep (or freezing preservation) with two-layer filtered through gauze, in filtrate, add strong alkali solution to PH11-11.5, be heated to and boil, be cooled to and transfer after the room temperature PH, add a certain amount of bilirubin polymerase-H again to neutral with hydrolysis, effect is about 30 minutes under the room temperature, add low concentration calcium hydroxide (or magnesium, zinc alkali) solution again, be heated to and boil several minutes, cooled and filtered, the washing filtering thing is removed behind the impurity the filtrate vacuum drying, promptly gets aggretion type bilirubin salt.
2, the preparation of new Calculus Bovis
By following prescription each composition is added abundant mix homogeneously by proportioning and promptly get new Calculus Bovis product.
Prescription (by weight): aggretion type bilirubin salt 40-65%
Cholic acid 7.0-10.0%
Taurine 0.5-1.5%
Cholesterol 1.0-2.0%
Unconjugated bilirubin 0.5-0.7%
Each seed amino acid 3.0-5.0%
Trace element 1.0-2.0%
Bile dry powder adds to 100%
Embodiment 1: prepare aggretion type bilirubin salt with the bilirubin powder.
Get bilirubin 500g and add 95% ethanol 500ml and fully mix, add 10mmolNaOH solution 100L again, be stirred well to solution and be brown transparent, the reuse dilute hydrochloric acid transfers PH to neutral, adds bilirubin polymerase-H25 ten thousand units, fully mixes, effect is 30 minutes under the room temperature, adds the CaCl of 1mol again
2After solution 1L fully stirs 10 minutes, remove by filter liquid, filtrate again with washing-filtration-washing-filtration-vacuum drying, can be contained the aggretion type calcium bilirubinate 600g of total bilirubin more than 80% by the filter cloth more than 400 orders.
Embodiment 2: with the direct synthesized polymer type of bile bilirubin salt.
Fel Bovis seu Bubali with fresh or freezing preservation; with two-layer filtered through gauze; get this bile 100kg and add N NaOH6.5L accent PH11-11.5; be heated to and boil with the water aquatic foods; cease fire after 5 minutes and be cooled to room temperature accent PH to neutral; add bilirubin polymerase-H2.5 ten thousand units, fully stir 30 minutes after, in solution, add 50mmol Ca (OH)
2Solution 1L, reheat kept to boiling 5 minutes, and filter with 400 order filter clothes the cooling back, for several times, removes impurity with the filtrate vacuum drying repeatedly, can contain the aggretion type calcium bilirubinate 60g of total bilirubin more than 80%.
With embodiment gained aggretion type bilirubin salt, can get new Calculus Bovis product after by the proportioning mix homogeneously by prescription and other compositions.
Medicine stability, drug effect and the toxicological effect of the new Calculus Bovis of gained of the present invention and artificial Calculus Bovis, natural Calculus Bovis contrast and experiment are as follows:
1, the animal toxicity of new Calculus Bovis experiment:
(1) acute toxicity testing:＞2.0g/kg body weight, be equivalent to 333 times of clinical application, acute toxicity (LD50) toxicity grading belongs to nontoxic substantially.
(2) long term toxicity test: new Calculus Bovis is to the hematuria routine of rat, and liver, renal function and the heart, liver, kidney, lung, organizing of spleen all do not have influence.
2, the effect experiment of new Calculus Bovis:
Use consistent and be better than the artificial Calculus Bovis at calm, analgesic, antiinflammatory and spasmolytic with natural Calculus Bovis.Use at spasmolytic and to be better than natural Calculus Bovis.
3, the stability of new Calculus Bovis:
(1) reserved sample observing: placed room temperature 6 months, its character, identification experiment and content (total bilirubin and total bile acid) are measured no change.
(2) chemical kinetics experiment: adopt the constant temperature accelerated test, the new Calculus Bovis of prediction is effect duration more than 5.2 years at room temperature.
Product quality checks every index all to meet the standard code of 92 years version Chinese Pharmacopoeias through the institute for drug control, Jilin Province.
Claims (3)
1, a kind of manufacture method of new Calculus Bovis is characterized in that aggretion type bilirubin salt and cholic acid, taurine, cholesterol, unconjugated bilirubin, each seed amino acid, trace element, bile dry powder blend are formed, and its prescription (by weight) is:
Aggretion type bilirubin salt 40-65%
Cholic acid 7.0-10.0%
Taurine 0.5-1.5%
Cholesterol 1.0-2.0%
Unconjugated bilirubin 0.5-0.7%
Each seed amino acid 3.0-5.0%
Trace element 1.0-2.0%
Bile dry powder adds to 100%
2, new Calculus Bovis manufacture method as claimed in claim 1; it is characterized in that aggretion type bilirubin salt can prepare with the bilirubin powder: bilirubin is added in rare alkali ethanol water make its dissolving; transfer PH to neutral with diluted acid; add a certain amount of bilirubin polymerase-H; effect is about 30 minutes under the room temperature, adds calcium salt (or magnesium, zinc salt) again and fully stirs, then repeatedly behind the washing filtering; with filtrate vacuum drying at room temperature, promptly get aggretion type calcium bilirubinate (or magnesium, zinc salt).
3; new Calculus Bovis manufacture method as claimed in claim 1; it is characterized in that aggretion type bilirubin salt can be directly synthetic with bile: with cattle; the bright bile of pig or sheep (or freezing preservation) with two-layer filtered through gauze; in filtrate, add strong alkali solution to PH11-11.5; be heated to and boil with hydrolysis; transfer PH to neutral after being cooled to room temperature; add a certain amount of bilirubin polymerase-H again; effect is about 30 minutes under the room temperature; add low concentration calcium hydroxide (or magnesium again; zinc alkali) solution; be heated to and boil several minutes; cooled and filtered, washing filtering thing are removed behind the impurity the filtrate vacuum drying, promptly get aggretion type bilirubin salt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95119587A CN1153635A (en) | 1995-12-04 | 1995-12-04 | Prepn of bezoar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95119587A CN1153635A (en) | 1995-12-04 | 1995-12-04 | Prepn of bezoar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1153635A true CN1153635A (en) | 1997-07-09 |
Family
ID=5082099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95119587A Pending CN1153635A (en) | 1995-12-04 | 1995-12-04 | Prepn of bezoar |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1153635A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006001721A1 (en) * | 2004-06-29 | 2006-01-05 | The Horticulture And Food Research Institute Of New Zealand Limited | Imprinted polmyer for binding organic molecules or metal ions |
| CN103316046A (en) * | 2013-06-09 | 2013-09-25 | 宁波优美肽生物科技有限公司 | Enzymatic production process for culturing calculus bovis in vitro |
-
1995
- 1995-12-04 CN CN95119587A patent/CN1153635A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006001721A1 (en) * | 2004-06-29 | 2006-01-05 | The Horticulture And Food Research Institute Of New Zealand Limited | Imprinted polmyer for binding organic molecules or metal ions |
| CN103316046A (en) * | 2013-06-09 | 2013-09-25 | 宁波优美肽生物科技有限公司 | Enzymatic production process for culturing calculus bovis in vitro |
| CN103316046B (en) * | 2013-06-09 | 2014-12-17 | 宁波优美肽生物科技有限公司 | Enzymatic production process for culturing calculus bovis in vitro |
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