CN115363058B - Disinfectant for effectively inactivating novel coronaviruses - Google Patents
Disinfectant for effectively inactivating novel coronaviruses Download PDFInfo
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- CN115363058B CN115363058B CN202110810090.0A CN202110810090A CN115363058B CN 115363058 B CN115363058 B CN 115363058B CN 202110810090 A CN202110810090 A CN 202110810090A CN 115363058 B CN115363058 B CN 115363058B
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- water
- ammonium chloride
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Abstract
The application discloses a novel disinfectant for effectively inactivating novel coronaviruses, which comprises the following raw materials in parts by weight: 20-32 parts of natural plant extract, 0.4-0.7 part of food-grade active agent, 2-3 parts of compound quaternary ammonium chloride, 0.2-0.4 part of lysostaphin, 3-5 parts of eucalyptol, 1-2 parts of hydroxypropyl starch and 58-64 parts of water, wherein the novel disinfectant is prepared by steps of multistage mixing, pH value adjustment and the like.
Description
Technical Field
The invention belongs to the technical field of disinfectant preparation, and particularly relates to a novel disinfectant for effectively inactivating novel coronaviruses, a preparation method and a use method thereof.
Background
Along with the global spread of new crown epidemic situation, disinfection is moved from a market segment to mass market, epidemic prevention disinfection is moved to normalization step by step.
The main types of disinfectants currently used in the market for killing new crowns are three main types:
1. chlorine-containing disinfectants, typically 84 disinfectants on the market, are most widely used due to low price;
2. peroxide disinfectants, hydrogen peroxide and peroxyacetic acid in the market are more used in the field of medical professional disinfection due to the advantage of strong oxidizing property;
3. alcohol disinfectant, which is typically 75% alcohol disinfectant in market, has reasonable price and better effect, and is more used in domestic markets such as household disinfection, hand disinfection and the like.
However, these disinfectants have limitations in terms of performance or application in the face of epidemic prevention in new crown epidemic situations:
the biggest disadvantage of 84 disinfectants is that the disinfectant is toxic and has strong oxidizing property, and chlorine generated after decomposition is toxic, and can only be used for disinfecting an open place, but cannot be used for disinfecting a closed space, such as an elevator cabin, and particularly cannot be used for disinfecting skin, mucous membranes, clothes, metal surfaces and other places. So 84 disinfectant is not usable in the strict sense of all articles that are directly sterilized by a person or in close contact with a person.
Peroxide disinfectants, most commonly hydrogen peroxide with concentration of 3% -5%, have strong irritation, corrosiveness and bleaching property, and hydrogen peroxide disinfectant with concentration of 0.5% can realize disinfection and sterilization of new coronavirus without obvious side effect, but the hydrogen peroxide disinfection technology with concentration of 0.5% and stability has higher difficulty and higher realization cost than the disinfectant of the invention, so that the peroxide disinfectant is more used for high-end disinfection application in operating rooms and the like.
The 75% alcohol disinfectant can be used for directly disinfecting human body or metal, and has the defects of irritation, flammability and allergen. If the fabric is electrostatically charged, it is extremely liable to cause a fire, and thus cannot be sprayed for sterilization. Taking an airplane, a high-speed railway and an automobile, the disinfectant containing alcohol is not allowed to be carried.
In the new epidemic situation, people, things and places need to be thoroughly disinfected, most disinfectants have various limitations, the combined use brings technical complexity and security holes in implementation, the cost is high, and a safe and broad-spectrum efficient disinfectant is urgently needed in the market.
Disclosure of Invention
The invention provides a novel disinfectant for effectively inactivating novel coronaviruses, a preparation method and a use method thereof, which are used for solving the problems of poor applicability of the existing disinfectant to inactivating novel coronaviruses and the like.
In order to solve the technical problems, the invention adopts the following technical scheme:
the novel disinfectant for effectively inactivating the novel coronavirus comprises the following raw materials in parts by weight: 20-32 parts of natural plant extract, 0.4-0.7 part of food-grade active agent, 2-3 parts of compound quaternary ammonium chloride compound, 0.2-0.4 part of lysostaphin, 3-5 parts of eucalyptol, 1-2 parts of hydroxypropyl starch and 58-64 parts of water.
Preferably, the novel disinfectant for effectively inactivating the novel coronavirus comprises the following raw materials in parts by weight: 28 parts of natural plant extract, 0.6 part of food-grade active agent, 2.4 parts of compound quaternary ammonium chloride compound, 0.3 part of lysostaphin, 4 parts of eucalyptol, 1.6 parts of hydroxypropyl starch and 60 parts of water.
Preferably, the natural plant extract comprises one or more of tea tree essential oil, eucalyptus essential oil, juniper essential oil, grape seed extract, castor extract and hibiscus extract.
Preferably, the food grade active agent comprises erucic acid.
Preferably, the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride according to the weight ratio of 2-4:5-7:1-2.
Preferably, the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride according to the weight ratio of 3:6.2:1.4.
Preferably, the water comprises one or more of distilled water, purified water, and fresh cool boiled water.
The invention also provides a preparation method of the novel disinfectant for effectively inactivating the novel coronavirus, which comprises the following steps:
s1: mixing natural plant extract, food-grade active agent and water, heating to 55-63deg.C, stirring at 200-300r/min for 7-10min to obtain mixed solution A;
s2: cooling the mixed solution A prepared in the step S1 to 32-45 ℃, adding a compound quaternary ammonium chloride compound, and stirring for 6-8min at the microwave power of 100-200W and the rotating speed of 300-400r/min to prepare mixed solution B;
s3: and (2) adding lysostaphin, eucalyptol and hydroxypropyl starch into the mixed solution B prepared in the step (S2), stirring for 5-6min at the temperature of 46-52 ℃ and the temperature of 200-300r/min under the pressure of 1-2MPa, and then regulating the pH value to obtain the novel disinfectant for effectively inactivating the novel coronavirus.
Preferably, the pH in step S3 is between 6 and 6.8.
The invention also provides a use method of the novel disinfectant for effectively inactivating the novel coronavirus, which comprises the steps of diluting the novel disinfectant to a mass concentration not lower than two parts per million by adopting one or more of distilled water, purified water and fresh cool boiled water, and then spraying the novel disinfectant into the air or onto the surface of an object in an atomization mode, so that the novel coronavirus can be effectively inactivated.
The invention has the following beneficial effects:
1. the invention utilizes the stability of the protein capsid interfering the surface of the novel coronavirus contained in the natural plant extract to achieve the killing effect, and utilizes the surfactant property of the quaternary ammonium salt compound to destroy the lipid layer on the surface of the novel coronavirus so as to lose various composite killing effects such as the infection capability to human cells.
2. The lysostaphin, the citrinol and the hydroxypropyl starch play a synergistic role in preparing the novel disinfectant disclosed by the invention, and the inactivation efficiency of the novel coronavirus is synergistically improved, because: the lysostaphin generates leakage holes by destroying proteins of cell membranes of the novel coronaviruses, so that normal functions of the viruses are interfered, nutrient loss of the novel coronaviruses is caused, and the novel coronaviruses die gradually, and the novel coronaviruses are matched with the citrinol for use, so that the citrinol can form a supermolecular stable structure with the lysostaphin, and the stability and lasting virucidal effect of the novel disinfectant are further improved; meanwhile, the eucalyptol has extremely strong virucidal capability, can change the potential of the virus surface, can enhance the penetration capability of lysostaphin, and further enhances the virucidal effect; the hydroxypropyl starch has good viscosity, and after the novel disinfectant disclosed by the invention is used, the novel disinfectant can be adhered to the surface of a novel coronavirus, so that interaction between the eucalyptol citrate and the lysostaphin is promoted, the lasting virucidal effect is enhanced, and the inactivation efficiency of the novel coronavirus is improved.
The specific action principle is as follows: staphylococcus aureus is a gram-positive bacterium, peptidoglycan is an important component of its cell wall, maintains cell morphology, and protects cells from osmotic swelling. Staphylococcus aureus peptidoglycans are composed of alternating N-acetylglucosamine and N-acetylmuramic acid residues, muramic acid is linked to tetrapeptides (D-alanine, D-glutamine, L-lysine, D-alanine) and tetrapeptides and glycine pentapeptides are linked together by epsilon-amino groups of single-chain lysine residues and on the other side by alanine carboxyl groups. Lysostaphin first synthesizes a preproenzyme of 493 amino acids, which is directed into the secretory pathway by a leader peptide of 36 amino acids at the N-terminus, and secreted extracellularly. The N-terminus of the pro-enzyme has 15 repeats of 13 amino acids which provide a lysostaphin pre-enzyme source with 70% less activity than mature lysostaphin. These repeats form active lysostaphin upon degradation by secreted cysteine proteases. Mature lysostaphin molecules consist of two parts: an N-terminal protease region (PD) having protease catalytic activity; c-terminal cell wall targeting region (CWT), CWT guides lysostaphin to bind to the receptor on the surface of staphylococcus aureus, acts on Gly pentapeptide bridging in the crosslinked structure of peptidoglycan of cell wall of staphylococcus aureus, so as to crack cells and achieve the effect of wall breaking and bacteriolysis.
3. The novel disinfectant has the following advantages:
(1) The sterilization concentration is lower, and two parts per million are generally used;
(2) The product has no irritation and toxicity;
(3) The solvent has faint scent slightly, and has no corrosion and bleaching effects on the surface of an object;
(4) Broad spectrum sterilization, lasting for more than 8 hours;
(5) Stable property, light resistance, heat resistance and easy storage.
4. The novel disinfectant provided by the invention is used in the scene and market channel:
(1) The sterilizing device can be applied to environmental sterilization in unit office and enterprise production;
(2) The sterilizing agent can be applied to environmental sterilization of school places such as classrooms, dormitories, dining halls, libraries and the like;
(3) The sterilizing device can be applied to the sterilization of public transportation means such as subways, buses, taxis and the like;
(4) Can be applied to the environmental disinfection of hospitals and medical institutions;
(5) The sterilizing agent can be applied to environmental sterilization of market supermarkets, entertainment venues, amusement parks, movie theatres and the like;
(6) The method can be applied to the environment disinfection of communities and residential communities;
(7) Can be used for sterilizing household and personal, such as clothing, furniture, hand skin, etc.
5. The novel disinfectant disclosed by the invention is nontoxic, harmless, noncorrosive and biodegradable, has the advantages of high speed of killing novel coronaviruses and long duration of action, and has incomparable safety, broad spectrum and the like compared with the traditional disinfectant shown in the following table. The novel disinfectant disclosed by the invention is widely applied to the disinfection of European and American health institutions, school communities, transportation hubs, logistics storage and other scenes on the aspect of preventing novel coronaviruses of personnel, articles, facilities and the like.
Detailed Description
For a better understanding of the present invention, the following examples are now to be taken as being within the scope of the present invention, but are not to be construed as limiting the scope of the present invention.
In this embodiment, the novel disinfectant for effectively inactivating a novel coronavirus comprises the following raw materials in parts by weight: 20-32 parts of natural plant extract, 0.4-0.7 part of food-grade active agent, 2-3 parts of compound quaternary ammonium chloride compound, 0.2-0.4 part of lysostaphin, 3-5 parts of eucalyptol, 1-2 parts of hydroxypropyl starch and 58-64 parts of water;
the natural plant extract liquid comprises one or more of tea tree essential oil, uganilli essential oil, eucalyptus essential oil, juniper essential oil, grape seed extract, castor extract and hibiscus extract;
the food grade active agent comprises erucic acid;
the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride according to the weight ratio of 2-4:5-7:1-2;
the water comprises one or more of distilled water, purified water and fresh cool boiled water;
the preparation method of the novel disinfectant for effectively inactivating the novel coronavirus comprises the following steps:
s1: mixing natural plant extract, food-grade active agent and water, heating to 55-63deg.C, stirring at 200-300r/min for 7-10min to obtain mixed solution A;
s2: cooling the mixed solution A prepared in the step S1 to 32-45 ℃, adding a compound quaternary ammonium chloride compound, and stirring for 6-8min at the microwave power of 100-200W and the rotating speed of 300-400r/min to prepare mixed solution B;
s3: and (2) adding lysostaphin, eucalyptol and hydroxypropyl starch into the mixed solution B prepared in the step (S2), stirring for 5-6min at the temperature of 46-52 ℃ and the temperature of 200-300r/min under the pressure of 1-2MPa, and then adjusting the pH value to 6-6.8 to prepare the novel disinfectant for effectively inactivating the novel coronavirus.
The novel disinfectant for effectively inactivating the novel coronavirus is diluted to a mass concentration not lower than two parts per million by one or more of distilled water, purified water and fresh cool boiled water, and then sprayed into the air or sprayed onto the surface of an object in an atomization mode, so that the novel coronavirus can be effectively inactivated.
The following is a description of more specific examples.
Example 1
The novel disinfectant for effectively inactivating the novel coronavirus comprises the following raw materials in parts by weight: 22 parts of natural plant extract, 0.5 part of food-grade active agent, 2 parts of compound quaternary ammonium chloride compound, 0.2 part of lysostaphin, 3.4 parts of citral, 1 part of hydroxypropyl starch and 59 parts of water;
the natural plant extract contains tea tree essential oil;
the food grade active agent comprises erucic acid;
the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride according to the weight ratio of 2:5:1;
the water is distilled water;
the preparation method of the novel disinfectant for effectively inactivating the novel coronavirus comprises the following steps:
s1: mixing natural plant extract, food-grade active agent and water, heating to 57 ℃, and stirring at a rotation speed of 200r/min for 10min to obtain a mixed solution A;
s2: cooling the mixed solution A prepared in the step S1 to 35 ℃, adding a compound quaternary ammonium chloride compound, and stirring for 8min at the microwave power of 100W and the rotating speed of 300r/min to prepare a mixed solution B;
s3: and (2) adding lysostaphin, eucalyptol and hydroxypropyl starch into the mixed solution B prepared in the step (S2), stirring for 6min at 48 ℃ under the pressure of 1MPa and the rotation speed of 200r/min, and then adjusting the pH value to 6.2 to obtain the novel disinfectant for effectively inactivating the novel coronavirus.
Example 2
The novel disinfectant for effectively inactivating the novel coronavirus comprises the following raw materials in parts by weight: 28 parts of natural plant extract, 0.6 part of food-grade active agent, 2.4 parts of compound quaternary ammonium chloride compound, 0.3 part of lysostaphin, 4 parts of eucalyptol, 1.6 parts of hydroxypropyl starch and 60 parts of water;
the natural plant extract liquid contains a castor extract and a cottonrose hibiscus extract, wherein the weight ratio of the castor extract to the cottonrose hibiscus extract is 4:3;
the food grade active agent comprises erucic acid;
the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride according to the weight ratio of 3:6.2:1.4;
the water is purified water;
the preparation method of the novel disinfectant for effectively inactivating the novel coronavirus comprises the following steps:
s1: mixing natural plant extract, food-grade active agent and water, heating to 60deg.C, and stirring at 300r/min for 7min to obtain mixed solution A;
s2: cooling the mixed solution A prepared in the step S1 to 42 ℃, adding a compound quaternary ammonium chloride compound, and stirring for 6min at the microwave power of 200W and the rotating speed of 400r/min to prepare a mixed solution B;
s3: and (2) adding lysostaphin, eucalyptol and hydroxypropyl starch into the mixed solution B prepared in the step (S2), stirring for 5min at the temperature of 50 ℃ and the pressure of 1.6MPa and the rotating speed of 300r/min, and then adjusting the pH value to 6.5 to prepare the novel disinfectant for effectively inactivating the novel coronavirus.
Example 3
The novel disinfectant for effectively inactivating the novel coronavirus comprises the following raw materials in parts by weight: 30 parts of natural plant extract, 0.7 part of food-grade active agent, 2.9 parts of compound quaternary ammonium chloride compound, 0.4 part of lysostaphin, 4.8 parts of citral, 1.7 parts of hydroxypropyl starch and 63 parts of water;
the natural plant extract contains grape seed extract;
the food grade active agent comprises erucic acid;
the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride according to the weight ratio of 4:7:2;
the water is fresh cool boiled water;
the preparation method of the novel disinfectant for effectively inactivating the novel coronavirus comprises the following steps:
s1: mixing natural plant extract, food-grade active agent and water, heating to 62deg.C, and stirring at 300r/min for 7min to obtain mixed solution A;
s2: cooling the mixed solution A prepared in the step S1 to 44 ℃, adding a compound quaternary ammonium chloride compound, and stirring for 7min at the microwave power of 160W and the rotating speed of 400r/min to prepare a mixed solution B;
s3: and (2) adding lysostaphin, eucalyptol and hydroxypropyl starch into the mixed solution B prepared in the step (S2), stirring for 6min at the temperature of 47 ℃ and the rotation speed of 200r/min under the pressure of 2MPa, and then adjusting the pH value to 6.7 to obtain the novel disinfectant for effectively inactivating the novel coronavirus.
Comparative example 1
The process was essentially the same as that of example 2, except that the novel disinfectant was prepared lacking lysostaphin, eucalyptol and hydroxypropyl starch as raw materials.
Comparative example 2
The process was essentially the same as that of example 2, except that the novel disinfectant was prepared with the exception that lysostaphin was absent from the starting material.
Comparative example 3
The process was essentially the same as that of example 2, except that the novel disinfectant was prepared with the lack of eucalyptol as the starting material.
Comparative example 4
The process was essentially the same as that of example 2, except that the novel disinfectant was prepared with the lack of hydroxypropyl starch as the starting material.
Test for inactivation of novel coronaviruses in vitro by disinfectant
1. Purpose of test
The disinfectant was tested for its inactivating effect on the novel coronavirus SARS-CoV-2.
2. Material
(1) Test sample
Disinfectants prepared in examples 1-3 and comparative examples 1-4.
(2) Cells
Vero cells were obtained from ATCC in the united states and cultured in MEM medium (Invitrogen) containing 10% fbs. Vero cells were counted after digestion with pancreatin digest and cell concentrations were adjusted to 2×10 with MEM medium containing 2% fbs 5 /mL。
(3) Virus (virus)
The novel coronavirus is obtained by inoculating Vero cells to sputum samples from patients with clinical acute stage of novel coronapneumonia, is determined to be novel coronavirus by fluorescence PCR (Cat No. Z-RR-0479, national mechanical injection 20203400057, shanghai river Biotechnology Co., ltd.) and viral whole gene sequencing (GSAID, hCoV-19/Hangzhou/ZJU-05/2020Vero EPI_ISL_415711), and other respiratory viruses are excluded from the culture by fluorescence PCR. Virus titres were determined by cytopathic observations and diluted to 2X 10 6 TCID 50 /mL。
3. Method and results
(1) Method for testing inactivation of disinfectant on new coronavirus
In the sterile 7 sample tubes, marks 1-7 respectively, adds the diluted disinfectants prepared in examples 1-3 and comparative examples 1-4 respectively (all adopt purified water to dilute the disinfectants to the mass concentration of two parts per million) to 100uL, then adds 2X 106 TCID50/mL of new coronavirus liquid 10uL into the corresponding marked sample tubes respectively, mixes gently and incubates at room temperature for 15min. After a predetermined time point of action, the disinfectant/virus mixture was transferred to 6-well cell cultureInto the plates, 4mL of Vero cell suspension (2X 10) was added immediately 5 /mL), placed in CO 2 The incubator continues to incubate for 72 hours.
(2) Novel coronavirus nucleic acid detection in culture solution
The real-time fluorescence quantitative PCR method is adopted. 200. Mu.L of the cell culture supernatant was aspirated, and viral nucleic acid was extracted with a magnetic bead nucleic acid extraction kit (MVR 01) and a full-automatic nucleic acid extractor (EX 3600, shanghai, river Biotechnology Co., ltd.) to give a final elution volume of 50. Mu.L. Taking 5 mu L of nucleic acid extract, adopting a novel coronavirus nucleic acid detection kit (fluorescent PCR method, product number Z-RR-0479, national standard 20203400057, shanghai river Biotechnology Co., ltd.) by a one-step method to detect the level of virus nucleic acid, and calculating the inhibition or inactivation efficiency of a sample to be detected on the virus according to the Ct value. [ Ct= -3.33Log 10 C(copies/mL)+42.03]. The results are shown in the following table.
Remarks: the above experiments involving live virus manipulation were all performed in the biosafety tertiary laboratory (BSL-3).
4. Conclusion analysis
From the above table, it can be seen that: (1) After the disinfectant prepared in the examples 1-3 is diluted to the mass concentration of two parts per million, the novel coronavirus SARS-CoV-2 can be effectively inactivated within 15 minutes, and the inactivation efficiency is more than 99.9%.
(2) From the inactivation efficiency data of the novel coronaviruses of example 2 and comparative examples 1-4, it can be calculated that the effect value of the inactivation efficiency of the novel coronaviruses produced when lysostaphin, eucalyptol citrate and hydroxypropyl starch are used together is improved by a percentage=15.02% > 10% compared with the effect value of the inactivation efficiency of the novel coronaviruses produced by superposition of lysostaphin, eucalyptol citrate and hydroxypropyl starch when the lysostaphin, eucalyptol citrate and hydroxypropyl starch are used separately, respectively, because the improved percentage is more than 10%, it is explained that the lysostaphin, eucalyptol and hydroxypropyl starch play a synergistic effect in preparing the novel disinfectant, and the inactivation efficiency of the novel coronaviruses is synergistically improved, because:
the lysostaphin can interfere with the normal function of the novel coronavirus to cause the nutrient loss of the novel coronavirus and further cause the gradual death of the novel coronavirus, and the novel coronavirus is matched with the citrinol for use, so that the citrinol can form a supermolecular stable structure with the lysostaphin, and the stability and the lasting virucidal effect of the novel disinfectant are further improved; meanwhile, the eucalyptol has extremely strong virucidal capability, can change the potential of the virus surface, can enhance the penetration capability of lysostaphin, and further enhances the virucidal effect; the hydroxypropyl starch has good viscosity, and after the novel disinfectant disclosed by the invention is used, the novel disinfectant can be adhered to the surface of a novel coronavirus, so that interaction between the eucalyptol citrate and the lysostaphin is promoted, the lasting virucidal effect is enhanced, and the inactivation efficiency of the novel coronavirus is improved.
The above description should not be taken as limiting the practice of the invention to these descriptions, but it will be understood by those skilled in the art that several simple deductions or substitutions may be made without departing from the spirit of the invention, and the invention is defined by the appended claims.
Claims (7)
1. The disinfectant for effectively inactivating the novel coronavirus is characterized by comprising the following raw materials in parts by weight: 20-32 parts of natural plant extract, 0.4-0.7 part of food-grade active agent, 2-3 parts of compound quaternary ammonium chloride compound, 0.2-0.4 part of lysostaphin, 3-5 parts of eucalyptol, 1-2 parts of hydroxypropyl starch and 58-64 parts of water;
the natural plant extract liquid comprises one or more of tea tree essential oil, grape seed extract, castor extract and cottonrose hibiscus extract;
the food grade active agent comprises erucic acid;
the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride according to the weight ratio of 2-4:5-7:1-2.
2. The disinfectant for effectively inactivating novel coronaviruses according to claim 1, comprising the following raw materials in parts by weight: 28 parts of natural plant extract, 0.6 part of food-grade active agent, 2.4 parts of compound quaternary ammonium chloride compound, 0.3 part of lysostaphin, 4 parts of eucalyptol, 1.6 parts of hydroxypropyl starch and 60 parts of water.
3. The disinfectant for effectively inactivating novel coronaviruses according to claim 1, wherein the compound quaternary ammonium chloride compound consists of cetylpyridinium chloride, didecyl dimethyl ammonium chloride and benzalkonium chloride in a weight ratio of 3:6.2:1.4.
4. The disinfectant effective to inactivate a novel coronavirus according to claim 1, wherein the water comprises one or more of distilled water, purified water, fresh cool boiled water.
5. A method of preparing a disinfectant effective to inactivate a novel coronavirus according to any one of claims 1-4 comprising the steps of:
s1: mixing natural plant extract, food-grade active agent and water, heating to 55-63deg.C, stirring at 200-300r/min for 7-10min to obtain mixed solution A;
s2: cooling the mixed solution A prepared in the step S1 to 32-45 ℃, adding a compound quaternary ammonium chloride compound, and stirring for 6-8min at the microwave power of 100-200W and the rotating speed of 300-400r/min to prepare mixed solution B;
s3: and (2) adding lysostaphin, eucalyptol and hydroxypropyl starch into the mixed solution B prepared in the step (S2), stirring for 5-6min at the temperature of 46-52 ℃ and the temperature of 200-300r/min under the pressure of 1-2MPa, and then regulating the pH value to obtain the disinfectant for effectively inactivating the novel coronavirus.
6. The method of claim 5, wherein the pH in step S3 is 6-6.8.
7. A method of using the disinfectant for effectively inactivating a novel coronavirus prepared by the method of claim 5 or 6, wherein the disinfectant is diluted to a mass concentration of not less than two parts per million by one or more of distilled water, purified water and fresh cool boiled water, and then sprayed into the air or onto the surface of an object by an atomization method, thereby effectively inactivating the novel coronavirus.
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| CN104524557A (en) * | 2014-12-16 | 2015-04-22 | 杨陈 | Lysostaphin compound disinfection gel |
| CN107198769A (en) * | 2017-05-04 | 2017-09-26 | 艾伯尔生物科技（重庆）有限公司 | Oral disinfecting spray and preparation method thereof |
| CN111903709A (en) * | 2020-08-11 | 2020-11-10 | 北京锦绣新技术发展有限公司 | Preparation method of sterilizing disinfectant for killing coronavirus |
| CN112618525A (en) * | 2020-12-18 | 2021-04-09 | 上海海禾医疗科技有限公司 | Disinfectant for quickly inactivating coronavirus |
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