CN115350271A - Bio-enzyme functional cotton sheet and preparation method thereof - Google Patents

Bio-enzyme functional cotton sheet and preparation method thereof Download PDF

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Publication number
CN115350271A
CN115350271A CN202211276214.2A CN202211276214A CN115350271A CN 115350271 A CN115350271 A CN 115350271A CN 202211276214 A CN202211276214 A CN 202211276214A CN 115350271 A CN115350271 A CN 115350271A
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percent
cotton sheet
bio
enzyme functional
solution
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叶贵子
黄青山
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Kunshan Biogreen Technology Co ltd
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Kunshan Biogreen Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/30—Zinc; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01017—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24075—Lysostaphin (3.4.24.75)

Abstract

The application relates to the technical field of biological sterilization, and particularly discloses a bio-enzyme functional cotton sheet and a preparation method thereof. A biological enzyme functional cotton sheet is prepared by loading sterilizing solution on a cotton sheet body; the sterilizing solution comprises the following raw materials in percentage by mass: 0.0001 to 1 percent of lysostaphin, 0.002 to 10 percent of lysozyme, 0.01 to 0.2 percent of nano zinc oxide, 10 to 40 percent of stabilizer, 1 to 20 percent of humectant, 0.1 to 2 percent of synergist and the balance of distilled water; the stabilizer is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio (100-200): 1 in a mixture. The bactericidal solution has an excellent and lasting bactericidal effect, is mild in property and free of stimulation, and is not easy to generate drug resistance; in addition, the cotton sheet body is loaded with the sterilizing solution to assist treatment, so that the practical effect is better.

Description

Bio-enzyme functional cotton sheet and preparation method thereof
Technical Field
The application relates to the technical field of biological sterilization, in particular to a biological enzyme functional cotton sheet and a preparation method thereof.
Background
The skin disease of pets is always a problem that pet owners feel headache, and after the pets are infected with the skin disease, the pets can continuously scratch itch on the bodies due to severe itch, so that the conditions of pimples, scurf, pachynsis, hair loss and the like are easily caused, and even serious diseases such as skin ulceration and the like are caused.
The common treatment method is to treat the infected area by using a sterilization preparation, and most of the sterilization preparations at present mainly comprise alcohol, iodine solution, potassium permanganate and the like as sterilization active ingredients. The bactericidal active ingredients have strong irritation to the skin, are easy to damage the skin after long-term use, and have the problem of easy generation of drug resistance.
In addition, the commonly used sterilizing preparation is usually spray, gel, cream and the like, wherein the sterilizing preparation of the spray preparation is influenced by pet hair, is not easy to be uniformly sprayed on infected skin areas, and has poor persistence; the gel and cream sterilization preparations need to be applied by hands, and have certain sanitation and infection hidden danger on the hand skin of a person who rubs the medicine on the premise of not wearing medical gloves.
Disclosure of Invention
In order to ensure the sterilization effect and simultaneously reduce the situations of irritation and drug resistance and provide a new treatment form for assisting treatment, the application provides a bio-enzyme functional cotton sheet and a preparation method thereof.
In a first aspect, the application provides a bio-enzyme functional cotton sheet, which adopts the following technical scheme:
a biological enzyme functional cotton sheet is prepared by loading sterilizing solution on a cotton sheet body; the sterilization solution comprises the following raw materials in percentage by mass: 0.0001 to 1 percent of lysostaphin, 0.002 to 10 percent of lysozyme, 0.01 to 0.2 percent of nano zinc oxide, 10 to 40 percent of stabilizer, 1 to 20 percent of humectant, 0.1 to 2 percent of synergist and the balance of distilled water; the stabilizer is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio (100-200): 1 in a mixture.
By adopting the technical scheme, the lysostaphin, the lysozyme and the nano zinc oxide are used as bactericidal active ingredients, and are mutually cooperated to achieve an excellent bactericidal effect, and the drug resistance is not easy to generate. Wherein the lysostaphin can act on the bacterial cell wall peptidoglycan pentaglycine peptide bridge, thereby destroying the bacterial cell wall and causing the bacterial death due to leakage of cell contents. Meanwhile, the lysostaphin core component contains zinc ions, so that the zinc content in local damaged skin and mucosal tissues can be increased, and the zinc ions can promote the biosynthesis of nucleic acid and protein, improve the activity of alkaline phosphatase (AKP) in local tissues, increase the content of collagen in the tissues and be beneficial to the growth and division of epidermal cells, so that the rapid healing of wounds of the damaged skin and the mucosal tissues is promoted, and the mucosal healing time is remarkably shortened.
Lysozyme can break the beta-1, 4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, so that the insoluble mucopolysaccharide of the cell wall is decomposed into soluble glycopeptide, and the content of the broken cell wall is escaped to dissolve the bacteria. Meanwhile, lysozyme can be directly combined with virus protein with negative charges and forms a complex with DNA, RNA and apoprotein to inactivate the virus.
The nano zinc oxide can continuously release zinc ions in an aqueous medium, the zinc ions can enter bacterial cell membranes and damage the cell membranes, and when the zinc ions react with certain groups of proteins in cells, the space structures of the bacteria and the proteins in the cells are damaged, so that protease in the cells is inactivated, and the bacteria are killed. And after the structure of the bacteria is damaged, zinc ions can be dissociated from the bacteria, and the sterilization process is repeated. In addition, the nano zinc oxide can interact with cell walls on the surfaces of bacteria, so that the cell walls of the bacteria are damaged, and the contents are released to kill the bacteria.
In conclusion, although the sterilization mechanism of lysostaphin and lysozyme is directed at cell wall peptidoglycan, the action sites are different, and the two have obvious synergistic bactericidal action after being compounded. The sterilization mechanism of the nano zinc oxide is greatly different from that of lysostaphin and lysozyme, and a repeated sterilization process exists, so that the nano zinc oxide has good sterilization duration; meanwhile, zinc ions released by the nano zinc oxide are matched with zinc ions in the lysostaphin, so that the rapid healing of the wound surfaces of damaged skin and mucosa tissues is promoted, and the mucosa healing time is obviously shortened. Therefore, the lysostaphin, the lysozyme and the nano zinc oxide are used as bactericidal active ingredients, and are cooperated with each other, so that the bactericidal effect is remarkably improved, and the bactericidal composition has a better positive significance for promoting wound healing, prolonging the drug effect and reducing the drug resistance. In addition, the three have little toxic action and irritation to organisms, and have better practical significance.
In addition, the stabilizer obtained by compounding according to the proportion can effectively improve the activity of lysostaphin and lysozyme and ensure the stability of bactericidal active ingredients. Specifically, the polyethylene glycol 400 can form a protective film on the surfaces of lysostaphin and lysozyme, so that the structures of the lysostaphin and the lysozyme are prevented from being damaged; and the polyethylene glycol 400 can effectively improve the viscosity of the sterilization solution, so that the sterilization solution is more easily attached to the skin infection area, and the effect of persistent sterilization is achieved. And the ethylene diamine tetraacetic acid can effectively chelate metal ions such as calcium, magnesium and the like which harm the enzymatic activity, so that the stability of the bactericidal active component is further guaranteed.
Meanwhile, the cotton cloth body is used as a loading medium of the sterilizing solution, so that the sterilizing solution can be transferred to the skin infection area conveniently and uniformly, the contact between the hand skin of a medicine applicator and the infection area is reduced, and the infection hidden danger is low.
In a specific embodiment, the bactericidal solution comprises the following raw materials in percentage by mass: 0.2 to 0.7 percent of lysostaphin, 3 to 8 percent of lysozyme, 0.04 to 0.12 percent of nano zinc oxide, 20 to 30 percent of stabilizer, 5 to 10 percent of humectant, 0.5 to 1 percent of synergist and the balance of distilled water; the stabilizer is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio (120-150): 1 in a mixture.
By adopting the technical scheme, the proportion of the sterilizing solution is further optimized, and the comprehensive performance of the sterilizing solution is further improved.
In a specific embodiment, the cotton piece body is obtained by pre-soaking in chitosan solution.
By adopting the technical scheme, firstly, the chitosan has a certain antibacterial and bactericidal effect, and can play a role of a stabilizing agent similar to polyethylene glycol 400. Meanwhile, chitosan has excellent hygroscopicity, and the cotton sheet body pre-impregnated with the chitosan solution can better adsorb the sterilization solution; and more sterilization solution is not directly contacted with the cotton sheet body, but tends to be combined with the chitosan solution, so that the residual quantity of sterilization active ingredients on the cotton sheet body in the wiping process can be effectively reduced, namely, the sterilization solution is more conveniently transferred to a skin infection area in the wiping process, and the positive significance is achieved for improving the treatment effect. In addition, the bactericidal active ingredient combined with the chitosan has better slow release effect, and can further prolong the drug effect.
In a specific embodiment, the chitosan solution has a mass concentration of 0.5% to 1%.
In a specific embodiment, the liquid content of the cotton piece body after being soaked in the chitosan solution is 0.1-0.3 times.
In a specific possible embodiment, the humectant is allantoin and ceramide in a mass ratio of 1: (3.6-5.2).
By adopting the technical scheme, the allantoin has a good moisturizing effect and a certain antibacterial effect; ceramide, however, has a strong ability to associate with water molecules, and can maintain skin moisture by forming a network structure in the stratum corneum. After the two are compounded according to the proportion, the moisturizing effect is more obvious.
In a specific possible embodiment, the synergistic agent is a mixture of climbazole and chitin according to a mass ratio of 1: (1.4-2.2).
By adopting the technical scheme, the climbazole has better broad-spectrum sterilization performance, and the chitin has better sterilization effect and moisturizing effect; meanwhile, the nature of the climbazole and the chitin is mild, and after the climbazole and the chitin are compounded according to the proportion, the synergistic effect is more obvious.
In addition, both the climbazole and the chitin are insoluble in water, namely, the climbazole and the chitin mainly exist in a solid state in a system, and the existence of solid particles in a film layer formed after the medicine is applied can endow the film layer with better ventilation effect.
In a second aspect, the application provides a method for preparing a bio-enzyme functional cotton sheet, which adopts the following technical scheme:
a preparation method of a biological enzyme functional cotton sheet comprises the following steps:
preparing a sterilization solution: premixing polyethylene glycol 400, ethylene diamine tetraacetic acid and distilled water, then adding lysostaphin, lysozyme and nano zinc oxide, and fully mixing to obtain a premixed solution; then adding the humectant and the synergist into the premixed solution, and fully mixing to obtain a sterilization solution;
and (3) load processing: and filling a sterilizing solution into the container containing the cotton sheet body to obtain the finished product of the biological enzyme functional cotton sheet.
In a specific possible embodiment, in the loading treatment step, the cotton piece body is firstly soaked in the chitosan solution, then the soaked cotton piece body is placed in a container, and then the sterilization solution is filled in the container, so that the finished product of the bio-enzyme functional cotton piece is obtained.
In a specific embodiment, in the loading treatment step, the liquid content of the bio-enzyme functional cotton piece is 1.7 to 1.9 times.
In summary, the present application has the following beneficial effects:
1. the sterilization active ingredients are lysostaphin, lysozyme and nano-zinc oxide, and as the sterilization action principles of the lysostaphin and the lysozyme are different, the lysostaphin and the lysozyme act on different sites on the bacterial cell wall, and the nano-zinc oxide destroys the cell wall, the cell membrane and the internal structure of the cell membrane, the three are compounded to have a remarkable sterilization synergistic effect; and the nano zinc oxide can be dissociated from the bacteria after the structure of the bacteria is destroyed, so that the effect of repeated sterilization is realized, namely the duration of the drug effect is longer.
2. The nano zinc oxide, the climbazole and the chitin tend to exist in a solid powder form in a system, and the existence of the solid powder in a film layer formed after the medicine is rubbed can endow the film layer with a better air permeation effect.
3. The cotton piece body with impregnating chitosan in advance is as the media of load bactericidal solution in this application, and more bactericidal solutions tend to combine with chitosan, and the in-process bactericidal solution of being convenient for wipe medicine shifts to skin infection region, and practical function is better.
Detailed Description
The present application will be described in further detail with reference to examples and comparative examples, and all of the starting materials referred to in the present application are commercially available.
Example 1
A biological enzyme functional cotton sheet is prepared by loading antiseptic solution on a cotton sheet body; the sterilizing solution comprises the following raw materials in percentage by mass: 0.5% of lysostaphin, 6% of lysozyme, 0.1% of nano zinc oxide, 30% of stabilizer, 8% of humectant, 1% of synergist and distilled water, wherein the balance is made up to 100%;
wherein the stabilizing agent is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio of 120: 1;
the grain size of the nano zinc oxide is 30-80 nm;
the humectant is composed of allantoin and ceramide according to a mass ratio of 1: 4.6;
the synergistic agent is composed of climbazole and chitin according to the mass ratio of 1:1.9 of the composition.
The preparation method of the biological enzyme functional cotton sheet comprises the following steps:
s1, preparing a sterilization solution: premixing polyethylene glycol 400, ethylene diamine tetraacetic acid and distilled water, then adding lysostaphin, lysozyme and nano zinc oxide, and fully mixing to obtain a premixed solution; then adding the humectant and the synergist into the premix solution, and fully mixing to obtain a sterilization solution;
s2, load processing: and filling a specified amount of sterilizing solution into the box filled with the cotton sheet body according to the specification that the liquid content of the finished product of the biological enzyme functional cotton sheet is 1.8 times, so as to obtain the finished product of the biological enzyme functional cotton sheet.
Examples 2 to 5 are different from example 1 in the ratio of each raw material, and are specifically shown in the following table.
Table 1 raw material proportioning table
Figure 52014DEST_PATH_IMAGE001
Example 6
The difference between the embodiment and the embodiment 1 is that the stabilizing agent is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio of 100:1, in a mixture of the components.
Example 7
The difference between the embodiment and the embodiment 1 is that the stabilizing agent is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio of 200:1, in a mixture of the components.
Example 8
The difference between the embodiment and the embodiment 1 is that the stabilizing agent is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio of 150:1 in a mixture.
Example 9
The difference between the embodiment and the embodiment 1 is that the moisturizers are allantoin and ceramide according to the mass ratio of 1: 3.6.
Example 10
The difference between the embodiment and the embodiment 1 is that the moisturizers are allantoin and ceramide according to the mass ratio of 1:5.2 of the composition.
Example 11
The embodiment is different from the embodiment 1 in that the moisturizers are allantoin and ceramide according to the mass ratio of 1:1 in a mixture.
Example 12
The difference between the embodiment and the embodiment 1 is that the moisturizers are allantoin and ceramide according to the mass ratio of 1:8 in the presence of a catalyst.
Example 13
This example differs from example 1 in that the humectant is allantoin.
Example 14
This example differs from example 1 in that the humectant is ceramide.
Example 15
The difference between the embodiment and the embodiment 1 is that the synergistic agent is the mixture of the climbazole and the chitin according to the mass ratio of 1: 1.4.
Example 16
The difference between the embodiment and the embodiment 1 is that the synergistic agent is the mixture of the climbazole and the chitin according to the mass ratio of 1:2.2 of the composition.
Example 17
The difference between the embodiment and the embodiment 1 is that the synergistic agent is the mixture of the climbazole and the chitin according to the mass ratio of 2:1 in a mixture.
Example 18
The difference between the embodiment and the embodiment 1 is that the synergistic agent is the mixture of the climbazole and the chitin according to the mass ratio of 1:4, and (b).
Example 19
The difference between this example and example 1 is that the synergist is galangin.
Example 20
The difference between this example and example 1 is that the synergistic agent is chitin.
Example 21
The difference between the present example and example 1 is that in the S2 loading treatment step, the cotton piece body is immersed in a chitosan solution with a mass concentration of 1%, and the liquid content of the immersed cotton piece body is 0.2 times; and then placing the impregnated cotton sheet body in a box, and filling a specified amount of sterilizing solution into the box according to the specification that the liquid content of the finished product biological enzyme functional cotton sheet is 1.8 times, so as to obtain the finished product biological enzyme functional cotton sheet.
Example 22
This example is different from example 21 in that the chitosan solution had a mass concentration of 0.5%.
Example 23
This example differs from example 21 in that the impregnated cotton sheet body had a liquid content of 0.1 times.
Example 24
This example differs from example 21 in that the impregnated cotton sheet body had a liquid content of 0.3 times.
Example 25
This example differs from example 21 in that the impregnated cotton sheet body had a liquid content of 0.6 times.
Example 26
This example is different from example 21 in that the content of the impregnated cotton sheet body was 0.05 times.
Comparative example 1
This comparative example differs from example 1 in that no lysozyme was added and the balance was made up with lysostaphin.
Comparative example 2
The present comparative example differs from example 1 in that lysostaphin is not added and the balance is made up with lysozyme.
Comparative example 3
This comparative example differs from example 1 in that no nano-zinc oxide was added and the balance was made up with lysostaphin.
Comparative example 4
This comparative example differs from example 1 in that no stabilizer is added and the balance is made up with distilled water.
Comparative example 5
This comparative example differs from example 1 in that no humectant was added and the balance was made up with distilled water.
Comparative example 6
The comparative example differs from example 1 in that no synergist was added and the balance was made up with distilled water.
Comparative example 7
This comparative example differs from example 1 in that the stabilizer is polyethylene glycol 400.
Comparative example 8
This comparative example differs from example 1 in that the stabilizer is ethylenediaminetetraacetic acid.
Comparative example 9
The comparative example is different from example 1 in that the stabilizing agent is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio of 20:1, in a mixture of the components.
Performance detection test method
And (3) sterilization test: the test is carried out according to GB15979-2002 appendix C3.2 of hygienic Standard for Disposable sanitary articles, and the test sample is the finished product bio-enzyme functional cotton sheet prepared in examples 1-26 and comparative examples 1-9.
And (3) stability test: the test is carried out according to the appendix C6 of GB15979-2002 hygienic Standard for Disposable sanitary articles, the test sample is the finished product bio-enzyme functional cotton piece prepared in the examples 1-26 and the comparative examples 1-9, the specific acceleration condition is that the cotton piece is placed in a constant temperature box at 37-40 ℃ for 3 months, and the temperature is kept to be more than 75%. And the finished product bio-enzyme functional cotton sheets in the accelerated examples and comparative examples are subjected to sterilization tests.
The treatment effect is as follows: the finished product bio-enzyme functional cotton sheets prepared in the examples 1-26 and the comparative examples 1-9 are used as preparations for treating folliculitis of pet cats, the finished product bio-enzyme functional cotton sheets of the examples and the comparative examples respectively correspond to pet cats with similar folliculitis symptom degrees, and the medicines are applied for 1 time per day for one week in the same application treatment mode. During the treatment process, continuous observation is carried out, no obvious inflammation in the application area is taken as a recovery standard, the continuous application of the medicine is stopped after the folliculitis is recovered, and the skin condition of the application area is observed.
TABLE 2 test results Table
Figure 437996DEST_PATH_IMAGE002
Figure 76787DEST_PATH_IMAGE003
By analyzing the test results in the table 2 and specifically combining the test results of the examples 1 to 5 and the test results of the comparative examples 1 to 3, the bio-enzyme functional cotton sheet prepared by the method has the advantages of excellent sterilization effect, good stability and good sterilization effect after 3 months of acceleration. And in the comparison examples 1-3, the lysozyme, the lysostaphin and the nano zinc oxide have obvious synergistic effect, and the sterilization effect of the bio-enzyme functional cotton sheet can be obviously improved by compounding.
According to the test results of the examples 1, 6 to 8, 4 and 7 to 9, the addition of the stabilizer can effectively improve the stability of lysozyme and lysostaphin and has positive significance for improving the sterilization effect. In addition, the polyethylene glycol 400 and the ethylene diamine tetraacetic acid have obvious compounding synergistic relationship, and the two have more obvious compounding effect according to a certain proportion.
According to the test results of the embodiment 1, the comparative example 3 and the comparative example 6, when the nano zinc oxide and the synergistic agent existing in a solid state in a system are not added, slight red swelling appears in the skin area after drug administration, which is probably caused by the fact that a film layer formed by the drug after the drug administration is airtight, so that the nano zinc oxide and the synergistic agent can effectively improve the air permeability of the drug while improving the sterilization effect, and have strong practical significance.
In view of the test results of example 1 and example 21, the chitosan solution was previously used to impregnate the cotton piece body, and the effect is significantly better in view of the actual therapeutic effect. Analysis revealed that the body of the cotton sheet pre-impregnated with the chitosan solution more readily transferred the antimicrobial active to the skin.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.

Claims (10)

1. A biological enzyme functional cotton sheet is characterized in that the biological enzyme functional cotton sheet is obtained by loading a sterilizing solution on a cotton sheet body; the sterilization solution comprises the following raw materials in percentage by mass: 0.0001 to 1 percent of lysostaphin, 0.002 to 10 percent of lysozyme, 0.01 to 0.2 percent of nano zinc oxide, 10 to 40 percent of stabilizer, 1 to 20 percent of humectant, 0.1 to 2 percent of synergist and the balance of distilled water; the stabilizer is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio (100-200): 1 in a mixture.
2. The bio-enzyme functional cotton piece as claimed in claim 1, wherein the bactericidal solution comprises the following raw materials by mass percent: 0.2 to 0.7 percent of lysostaphin, 3 to 8 percent of lysozyme, 0.04 to 0.12 percent of nano zinc oxide, 20 to 30 percent of stabilizer, 5 to 10 percent of humectant, 0.5 to 1 percent of synergist and the balance of distilled water; the stabilizer is polyethylene glycol 400 and ethylene diamine tetraacetic acid according to the mass ratio (120-150): 1, in a mixture of the components.
3. The bio-enzyme functional cotton sheet according to claim 1, wherein the cotton sheet body is obtained by pre-soaking in chitosan solution.
4. The bio-enzyme functional cotton piece as claimed in claim 3, wherein the mass concentration of the chitosan solution is 0.5% -1%.
5. The bio-enzyme functional cotton sheet according to claim 4, wherein the content of the liquid after the body of the cotton sheet is dipped in the chitosan solution is 0.1 to 0.3 times.
6. The bio-enzyme functional cotton sheet as claimed in claim 1, wherein the moisturizer is allantoin and ceramide in a mass ratio of 1: (3.6-5.2).
7. The bio-enzyme functional cotton piece as claimed in claim 1, wherein the synergistic agent is climbazole and chitin in a mass ratio of 1: (1.4-2.2).
8. A method for preparing a bio-enzyme functional cotton piece as described in any one of 1 to 7, comprising the following steps:
preparing a sterilization solution: premixing polyethylene glycol 400, ethylene diamine tetraacetic acid and distilled water, then adding lysostaphin, lysozyme and nano zinc oxide, and fully mixing to obtain a premixed solution; then adding the humectant and the synergist into the premixed solution, and fully mixing to obtain a sterilization solution;
and (3) load processing: and filling a sterilizing solution into the container containing the cotton sheet body to obtain the finished product of the biological enzyme functional cotton sheet.
9. The method for preparing bio-enzyme functional cotton sheets according to claim 8, wherein in the loading treatment step, the cotton sheet body is firstly soaked in the chitosan solution, then the soaked cotton sheet body is placed in a container, and then a sterilization solution is filled in the container, so that the finished bio-enzyme functional cotton sheets are obtained.
10. The method for preparing bio-enzyme functional cotton sheet according to claim 9, wherein in the loading step, the content of the bio-enzyme functional cotton sheet is 1.7 to 1.9 times.
CN202211276214.2A 2022-10-19 2022-10-19 Bio-enzyme functional cotton sheet and preparation method thereof Pending CN115350271A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1360949A (en) * 2000-12-28 2002-07-31 上海高科生物工程有限公司 Bactericidal gauze with lysostaphin complex enzyme

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1360949A (en) * 2000-12-28 2002-07-31 上海高科生物工程有限公司 Bactericidal gauze with lysostaphin complex enzyme

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牛妞 等: "抗菌蛋白酶敷料用于感染伤口的研究进展", 《护理学报》 *
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