CN115343131A - RNA detection device in exosome based on tumor diagnosis and prediction - Google Patents
RNA detection device in exosome based on tumor diagnosis and prediction Download PDFInfo
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- CN115343131A CN115343131A CN202211267631.0A CN202211267631A CN115343131A CN 115343131 A CN115343131 A CN 115343131A CN 202211267631 A CN202211267631 A CN 202211267631A CN 115343131 A CN115343131 A CN 115343131A
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- fixedly installed
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- rubber pad
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 15
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 9
- 238000003745 diagnosis Methods 0.000 title claims abstract description 9
- 238000001514 detection method Methods 0.000 title claims abstract description 8
- 230000007246 mechanism Effects 0.000 claims abstract description 60
- 238000003756 stirring Methods 0.000 claims abstract description 50
- 238000002347 injection Methods 0.000 claims description 14
- 239000007924 injection Substances 0.000 claims description 14
- 238000009434 installation Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 3
- 230000029142 excretion Effects 0.000 claims 1
- 239000007788 liquid Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 230000008569 process Effects 0.000 description 7
- 210000000988 bone and bone Anatomy 0.000 description 5
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 230000000630 rising effect Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- JGLMVXWAHNTPRF-CMDGGOBGSA-N CCN1N=C(C)C=C1C(=O)NC1=NC2=CC(=CC(OC)=C2N1C\C=C\CN1C(NC(=O)C2=CC(C)=NN2CC)=NC2=CC(=CC(OCCCN3CCOCC3)=C12)C(N)=O)C(N)=O Chemical compound CCN1N=C(C)C=C1C(=O)NC1=NC2=CC(=CC(OC)=C2N1C\C=C\CN1C(NC(=O)C2=CC(C)=NN2CC)=NC2=CC(=CC(OCCCN3CCOCC3)=C12)C(N)=O)C(N)=O JGLMVXWAHNTPRF-CMDGGOBGSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003419 tautomerization reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
Abstract
The invention relates to a tumor diagnosis and prediction-based RNA detection device in exosome, which belongs to the technical field of RNA detection and comprises a mounting frame, a beaker and a stirring mechanism, wherein the mounting frame is provided with a circular mounting plate, the stirring mechanism is mounted on the circular mounting plate, the beaker is mounted on the mounting frame, a rubber pad is placed at the bottom in the beaker, the lower end part of a concentration mechanism is circumferentially arrayed on the rubber pad, and the upper end part of the concentration mechanism is circumferentially arrayed on the circular mounting plate; the problems of high difficulty in manual operation and easiness in misoperation during sample treatment of RNA are solved.
Description
Technical Field
The invention belongs to the technical field of RNA detection, and particularly relates to a device for detecting RNA in exosomes based on tumor diagnosis and prediction.
Background
The principle of the ultraviolet absorption method is that purine and pyrimidine bases in molecular structures of nucleic acid, nucleotide and derivatives thereof have conjugated double bond systems and can strongly absorb ultraviolet light with the wavelength of 250 to 280nm, the maximum ultraviolet absorption value of the nucleic acid is 260nm, the content of nucleic acid substances can be detected from the change of ultraviolet light absorption values according to Lambert-Beer law, the ultraviolet absorption light shows obvious difference along with different tautomerism conditions of purine and pyrimidine bases in different PH solutions, and molar extinction coefficients of the ultraviolet absorption light and the nucleic acid are different.
In the operation process of sample treatment, 0.2-0.25g of crude RNA is required to be weighed, 2ml of 0.2% NaOH solution and 1ml of H2O solution are added to be mixed into paste, 40-50ml of H2O is added to be dissolved, the PH is adjusted to 7.0, then the volume is fixed to 100ml, the complex operation process usually needs manual operation, the operation difficulty is high, and operation errors are easy to occur.
Disclosure of Invention
The invention aims to provide a device for detecting RNA in exosome based on tumor diagnosis and prediction, which aims to solve the problems of great difficulty and easy misoperation in manual operation of RNA during sample treatment.
In order to achieve the above purpose, the specific technical scheme of the device for detecting RNA in exosome based on tumor diagnosis and prediction of the present invention is as follows:
a RNA detection device in exosome based on tumor diagnosis and prediction comprises a mounting frame, a beaker and a stirring mechanism, wherein a circular mounting plate is arranged on the mounting frame, the stirring mechanism is arranged on the circular mounting plate, the beaker is arranged on the mounting frame, a rubber pad is arranged at the bottom in the beaker, the lower end part of a concentration mechanism is circumferentially arrayed on the rubber pad, and the upper end part of the concentration mechanism is circumferentially arrayed on the circular mounting plate;
the centralized mechanism comprises a main helical gear, a bone rack rod, a connecting rod, a first hinge joint, a second hinge joint, a threaded rod, a first threaded part, a second threaded part, a sliding block, a first helical gear, a second helical gear and a first motor, wherein the bone rack rod is circumferentially arrayed in a groove formed in a rubber pad, the bone rack rod is hinged to one end of the connecting rod, the other end of the connecting rod is rotatably provided with the first hinge joint, the first hinge joint is hinged to the second hinge joint, the second hinge joint is rotatably installed in the groove formed in the threaded rod, the threaded rod penetrates through a through hole formed in the sliding block and is in threaded connection with the second helical gear, the second helical gear is rotatably installed in the groove formed in the sliding block, the second helical gear is meshed with the main helical gear, the main helical gear is fixedly installed at the output end of the first motor, the first motor is fixedly installed on the sliding block, the second helical gear is meshed with the first helical gear, the first helical gear is in threaded connection with the first threaded part, the first helical gear is in threaded connection with the second threaded part, the threaded rod is rotatably installed in the groove formed in the sliding block, and a bar-shaped protrusion is arranged in a groove formed in a circular mounting plate.
Further, the stirring mechanism comprises a second motor, a main gear, a stirring wheel and a stirring rod, the second motor is fixedly arranged on the circular mounting plate, the main gear is fixedly arranged at the output end of the second motor, insections meshed with the main gear are arranged on the stirring wheel, the stirring rod is fixedly arranged on the lower end face of the stirring wheel, and the lower end face of the stirring rod abuts against the upper end face of the rubber pad;
the puddler includes dead lever, inside spring and telescopic link, dead lever fixed mounting is on the lower terminal surface of stirring wheel, and dead lever slidable mounting is equipped with the inside spring that is used for supporting the dead lever in the recess that sets up on the telescopic link in the recess of telescopic link, and the lower tip of telescopic link supports on the up end of rubber pad.
Furthermore, the bottom of the beaker is provided with an output pipe for discharging, and the lower end of the rubber pad is provided with a rubber bulge for sealing the output pipe.
Further, the lifting mechanism is further included, the upper end portion of the lifting mechanism is installed on the installation frame, and the lower end portion of the lifting mechanism is installed on the rubber pad.
Further, it includes motor three, output gear, the ring gear, the lifting threaded rod, go up the articulated elements, articulated elements and bottom pole down, three fixed mounting of motor are in the through-hole that sets up on the mounting bracket, the output fixed mounting of motor three has the output gear who is used for driving the ring gear, ring gear and lifting threaded rod threaded connection, lifting threaded rod's recess internal rotation is installed and is used for the articulated elements of going up mutually with articulated elements down, the articulated elements is rotatory to be installed in the recess that sets up on the bottom pole down, bottom pole fixed mounting is in the recess that sets up on the rubber pad, be equipped with the through-hole that is used for lifting threaded rod to pass on the stirring wheel.
Further, still include convenient constant volume mechanism, convenient constant volume mechanism fixed mounting is on circular mounting panel.
Further, convenient constant volume mechanism includes the filling tube, the pneumatic cylinder, the control ring, the flexure strip, spring and rubber tube, filling tube fixed mounting is in the through-hole that sets up on circular mounting panel, pneumatic cylinder fixed mounting is in the through-hole that sets up on circular mounting panel, the lower terminal surface fixed mounting of circular mounting panel has the flexure strip, the output fixed mounting of pneumatic cylinder has the control ring, the intra-annular of control ring is arranged in to filling tube and flexure strip, be connected through the spring between flexure strip and the filling tube, fixed mounting has the rubber tube on the filling tube, be provided with the extension section on the rubber tube, the lower extreme and the extension section contact of flexure strip.
The invention has the advantages that:
1. the liquid metering device has the advantages that the rubber head dropper is required for metering the volume, the accuracy of the volume is ensured, the liquid surface in the container can shake in the liquid dropping process, the actual scale of the liquid in the container is not convenient to observe, the liquid can flow into the container by adhering the injection pipe to the inner wall of the beaker on the premise of ensuring the accuracy, the operation steps of metering the volume are reduced, and the speed of metering the volume is increased.
2. When the crude RNA is prepared manually, the crude RNA is spread and is not beneficial to centralized stirring, so that the stirring is not uniform.
Drawings
FIG. 1 is a schematic overall perspective view of the present invention;
FIG. 2 is a partial perspective view of the present invention;
FIG. 3 is a schematic perspective view of the concentrating mechanism of the present invention;
FIG. 4 is a schematic view of a part A in FIG. 3;
FIG. 5 is a schematic view of a portion B of FIG. 3;
FIG. 6 is a partial schematic view of the concentrating mechanism of the present invention;
FIG. 7 is a schematic view of a position relationship structure of the stirring mechanism of the present invention;
FIG. 8 is a schematic perspective view of the stirring mechanism of the present invention;
FIG. 9 is a schematic perspective view of the beaker of the present invention;
FIG. 10 is a perspective view of the rubber pad of the present invention;
FIG. 11 is a schematic view of a position relationship structure of the lifting mechanism of the present invention;
FIG. 12 is a schematic perspective view of the lifting mechanism of the present invention;
FIG. 13 is an enlarged partial view of C in FIG. 12;
FIG. 14 is a schematic view of a partial cross-sectional structure of the lifting mechanism of the present invention;
FIG. 15 is a schematic view of the construction of the bottom bar and rubber mat of the present invention;
FIG. 16 is a schematic view of a position relationship structure of the convenient constant volume mechanism of the present invention;
FIG. 17 is a schematic perspective view of the convenient volumetric mechanism of the present invention;
FIG. 18 is a schematic view in partial cross-section of a concentrating mechanism of the present invention;
FIG. 19 is a schematic view of a partial cross-sectional structure of the lift mechanism of the present invention;
FIG. 20 is a schematic view of the construction of the frame rod and rubber pad of the present invention;
fig. 21 is a partial sectional view schematically showing the structure of the stirring rod of the present invention.
The notation in the figure is:
a mounting frame 1; a circular mounting plate 11; a beaker 2; an output pipe 21; a stirring mechanism 3; a second motor 31; a main gear 32; a stirring wheel 33; insections 331; a stirring rod 34; a fixing lever 341; an inner spring 342; an extension rod 343; a concentration mechanism 4; the main helical gear 41; a skeletal frame rod 42; a connecting rod 43; a first hinge joint 44; a second hinge joint 45; a threaded rod 46; a first screw member 47; a second screw member 48; a slider 49; a through hole 491; a bar-shaped projection 492; a first bevel gear 410; a second bevel gear 411; a first motor 412; a rubber pad 5; a rubber projection 51; a lifting mechanism 6; a third motor 61; an output gear 62; a gear ring 63; a lifting threaded rod 64; an upper hinge 65; a lower hinge 66; a bottom bar 67; a convenient constant volume mechanism 7; an injection pipe 71; a hydraulic cylinder 72; the control loop 73; an elastic sheet 74; a spring 75; a rubber tube 76; an extension 761.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The first embodiment is as follows: as shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 18 and fig. 20, the RNA detection device in exosome based on tumor diagnosis and prediction comprises a mounting frame 1, beakers 2 and a stirring mechanism 3, wherein a circular mounting plate 11 is arranged on the mounting frame 1, the stirring mechanism 3 is arranged on the circular mounting plate 11, the beakers 2 are mounted on the mounting frame 1, rubber pads 5 are placed at the bottom in the beakers 2, the lower end parts of the concentration mechanisms 4 are circumferentially arrayed on the rubber pads 5, and the upper end parts of the concentration mechanisms 4 are circumferentially arrayed on the circular mounting plate 11; the concentration mechanism 4 comprises a main helical gear 41, a framework rod 42, a connecting rod 43, a first hinge joint 44, a second hinge joint 45, a threaded rod 46, a first threaded member 47, a second threaded member 48, a sliding block 49, a first helical gear 410, a second helical gear 411 and a first motor 412, wherein four framework rods 42 are circumferentially arrayed in a groove formed in a rubber pad 5, the framework rods 42 are hinged with one end of the connecting rod 43, the other end of the connecting rod 43 is rotatably provided with the first hinge joint 44, the first hinge joint 44 is hinged with the second hinge joint 45, the second hinge joint 45 is rotatably installed in a groove formed in the threaded rod 46, the threaded rod 46 penetrates through a through hole formed in the sliding block 49 to be in threaded connection with the second helical gear 411, the second helical gear 411 is rotatably installed in a groove formed in the sliding block 49, the second helical gear 411 is engaged with the main helical gear 41, the main helical gear 41 is fixedly installed at the output end of the first motor 412, the first motor 412 is fixedly installed on the sliding block 49, the second helical gear 411 is engaged with the first helical gear 410, the first helical gear 410 is in threaded connection with the first helical gear 47, the threaded rod 46 is positioned between the first threaded rod 48, a through hole 491, a through groove for sliding of the sliding block 49, and a through hole for mounting plate 11 is arranged on the sliding block 11, and a strip-shaped mounting plate 11;
so set up, starter motor 412, motor 412 drives the rotation of bevel gear two 411 through main bevel gear 41, bevel gear two 411 rotates and drives threaded rod 46 and the operation of bevel gear one 410 simultaneously, threaded rod 46 rebound passes through connecting rod 43 pulling hack lever 42, bevel gear one 410 moves on screw element one 47 and screw element two 48, thereby make slider 49 remove, finally realize four hack levers 42 and roll over rubber pad 5, make rubber pad 5 form the bowl form, when rabbling mechanism 3 stirs the pasty to crude RNA, more quick effective.
Example two: as shown in fig. 7, 8 and 21, the stirring mechanism 3 includes a second motor 31, a main gear 32, a stirring wheel 33 and a stirring rod 34, the second motor 31 is fixedly mounted on the circular mounting plate 11, the main gear 32 is fixedly mounted at an output end of the second motor 31, the stirring wheel 33 is provided with a tooth pattern 331 for meshing with the main gear 32, the stirring rod 34 is fixedly mounted at a lower end surface of the stirring wheel 33, and the lower end surface of the stirring rod 34 abuts against an upper end surface of the rubber pad 5; the stirring rod 34 comprises a fixed rod 341, an internal spring 342 and a telescopic rod 343, the fixed rod 341 is fixedly arranged on the lower end face of the stirring wheel 33, the fixed rod 341 is slidably arranged in a groove arranged on the telescopic rod 343, the internal spring 342 for supporting the fixed rod 341 is arranged in the groove of the telescopic rod 343, and the lower end part of the telescopic rod 343 abuts against the upper end face of the rubber pad 5;
with such an arrangement, the second motor 31 is started, the second motor 31 drives the stirring wheel 33 to rotate through the main gear 32, so that the stirring rod 34 rotates, the crude RNA is stirred in the rotation process of the stirring rod 34, the inner spring 342 can be compressed when the telescopic rod 343 is extruded, and the purpose of adjusting the overall length of the stirring rod 34 is achieved, so as to adapt to the height change of the rubber pad 5.
Example three: referring to the first embodiment, as shown in fig. 9, 10, 11, 12, 13, 14, 15 and 19, the bottom of the beaker 2 is provided with a delivery pipe 21 for discharging, and the lower end of the rubber pad 5 is provided with a rubber protrusion 51 for sealing the delivery pipe 21; the lifting mechanism 6 is further included, the upper end part of the lifting mechanism 6 is arranged on the mounting frame 1, and the lower end part of the lifting mechanism 6 is arranged on the rubber pad 5; the lifting mechanism 6 comprises a motor III 61, an output gear 62, a gear ring 63, a lifting threaded rod 64, an upper hinge part 65, a lower hinge part 66 and a bottom rod 67, the motor III 61 is fixedly arranged in a through hole arranged on the mounting rack 1, the output end of the motor III 61 is fixedly provided with the output gear 62 for driving the gear ring 63, the gear ring 63 is in threaded connection with the lifting threaded rod 64, the upper hinge part 65 hinged with the lower hinge part 66 is rotatably arranged in a groove of the lifting threaded rod 64, the lower hinge part 66 is rotatably arranged in a groove arranged on the bottom rod 67, the bottom rod 67 is fixedly arranged in a groove arranged on the rubber pad 5, and a through hole for the lifting threaded rod 64 to pass through is formed in the stirring wheel 33;
so set up, three 61 of starter motors, three 61 of motors drive gear ring 63 through output gear 62 and rotate, gear ring 63 makes lift threaded rod 64 rebound, lift threaded rod 64 pulling bottom pole 67 rebound makes the central point of bottom pole 67 pulling rubber pad 5 put, makes rubber bulge 51 break away from output tube 21, starts centralized mechanism 4 simultaneously, the rising speed of bottom pole 67 is greater than the rising speed of connecting rod 43, thereby make rubber pad 5 form the umbelliform, thereby the solution in the beaker 2 of being convenient for is arranged completely.
Example four: explaining on the basis of the first embodiment, as shown in fig. 16 and 17, the portable volume fixing device further comprises a portable volume fixing mechanism 7, and the portable volume fixing mechanism 7 is fixedly installed on the circular installation plate 11; the convenient constant volume mechanism 7 comprises an injection pipe 71, a hydraulic cylinder 72, a control ring 73, an elastic sheet 74, a spring 75 and a rubber pipe 76, wherein the injection pipe 71 is fixedly arranged in a through hole formed in the circular mounting plate 11, the hydraulic cylinder 72 is fixedly arranged in a through hole formed in the circular mounting plate 11, the elastic sheet 74 is fixedly arranged on the lower end surface of the circular mounting plate 11, the control ring 73 is fixedly arranged at the output end of the hydraulic cylinder 72, the injection pipe 71 and the elastic sheet 74 are arranged in the ring of the control ring 73, the elastic sheet 74 is connected with the injection pipe 71 through the spring 75, the rubber pipe 76 is fixedly arranged on the injection pipe 71, an extension section 761 is arranged on the rubber pipe 76, and the lower end of the elastic sheet 74 is contacted with the extension section 761;
so set up, start hydraulic cylinder 72, pneumatic cylinder 72 promotes control ring 73 and moves down, the in-process extrusion flexure strip 74 that control ring 73 removed, thereby make the extension 761 that sets up on the lower extreme extrusion rubber tube 76 of flexure strip 74, thereby make extension 761 press close to the inner wall of beaker 2, when reaching the constant volume, the inner wall inflow that can paste beaker 2 when liquid injects into beaker 2 through injection tube 71, can not cause stirring by a wide margin of beaker 2 interior liquid surface, more easily watch the concrete scale position at liquid place.
The working principle is as follows:
when a sample is processed, the crude RNA needs to be blended into paste, and when the sample is manually blended, the larger the spreading area of the crude RNA is along with the increase of the stirring amplitude, so that the problem of uneven stirring is caused. Starting the first motor 412, driving the second bevel gear 411 to rotate by the first motor 412 through the main bevel gear 41, driving the threaded rod 46 and the first bevel gear 410 to operate by the rotation of the second bevel gear 411, pulling the bone rack rods 42 by the upward movement of the threaded rod 46 through the connecting rod 43, driving the first bevel gear 410 to move on the first screw thread element 47 and the second screw thread element 48, so as to move the sliding block 49, finally realizing that the rubber pads 5 are folded by the four bone rack rods 42, forming the rubber pads 5 into a bowl shape, pouring crude RNA, naOH solution and H2O solution into the bowl-shaped rubber pads 5, starting the second motor 31, driving the stirring wheel 33 to rotate by the second motor 31 through the main gear 32, so as to rotate the stirring rod 34, and stirring the crude RNA in the rotating process of the stirring rod 34.
When the crude RNA is blended into paste, constant volume operation is required, liquid is generally directly injected into a container, the liquid with the stable surface is injected into the container through a rubber head dropper, the operation speed in the process is slow, when the fluctuation of the liquid surface is large, scale observation is not facilitated, the liquid is injected into the beaker 2 through the injection pipe 71, when a certain amount of liquid is injected, the hydraulic cylinder 72 is started, the hydraulic cylinder 72 pushes the control ring 73 to move downwards, the elastic sheet 74 is extruded in the moving process of the control ring 73, so that the lower end of the elastic sheet 74 extrudes the extension section 761 arranged on the rubber pipe 76, the extension section 761 is attached to the inner wall of the beaker 2, the flow direction of the injected liquid is changed, the extension section adheres to the inner wall of the beaker 2 to flow downwards, the liquid surface is small in fluctuation while the liquid is rapidly injected, the water level of the liquid is conveniently and accurately observed, and the purpose of improving the constant volume speed is achieved.
After the holding is accomplished, discharge beaker 2 interior solution, three 61 starting motor, three 61 motor drive gear ring 63 through output gear 62 and rotate, gear ring 63 makes lift threaded rod 64 rebound, lift threaded rod 64 pulling bottom pole 67 rebound, make bottom pole 67 pulling rubber pad 5's central point put, make rubber bulge 51 break away from output tube 21, start centralized mechanism 4 simultaneously, bottom pole 67's rising speed is greater than the rising speed of connecting rod 43, when 5 intermediate positions of rubber pad upwards promote this moment, four connecting rods 43 extrude rubber pad 5 to the centre, thereby make rubber pad 5 form the umbelliform, make solution can not stop on rubber pad 5, realize the clean discharge of the solution in the beaker 2 of being convenient for, rubber pad 5 is at the in-process that rises, telescopic link 343 extrudees inside spring 342, the length shrink of puddler 34, thereby do not influence the position change of rubber pad 5.
It is to be understood that the present invention has been described with reference to certain embodiments and that various changes in form and details may be made therein by those skilled in the art without departing from the spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the essential scope thereof. Therefore, it is intended that the invention not be limited to the particular embodiment disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
Claims (7)
1. The RNA detection device in exosome based on tumor diagnosis and prediction comprises a mounting rack (1), a beaker (2) and a stirring mechanism (3), wherein a circular mounting plate (11) is arranged on the mounting rack (1), the stirring mechanism (3) is arranged on the circular mounting plate (11), and the beaker (2) is arranged on the mounting rack (1), and is characterized in that a rubber pad (5) is placed at the bottom in the beaker (2), the lower end part of a concentration mechanism (4) is circumferentially arrayed on the rubber pad (5), and the upper end part of the concentration mechanism (4) is circumferentially arrayed on the circular mounting plate (11);
the concentration mechanism (4) comprises a main helical gear (41), a framework rod (42), a connecting rod (43), a first hinged joint (44), a second hinged joint (45), a threaded rod (46), a first threaded part (47), a second threaded part (48), a sliding block (49), a first helical gear (410), a second helical gear (411) and a first motor (412), wherein the four framework rods (42) are circumferentially arrayed in a groove formed in a rubber pad (5), the framework rods (42) are hinged with one end of the connecting rod (43), the other end of the connecting rod (43) is rotatably provided with the first hinged joint (44), the first hinged joint (44) is hinged with the second hinged joint (45), the second hinged joint (45) is rotatably arranged in the groove formed in the threaded rod (46), the threaded rod (46) penetrates through a through hole formed in the sliding block (49) to be in threaded connection with the second helical gear (411), the second helical gear (411) is rotatably arranged in the groove formed in the sliding block (49), the second helical gear (411) is meshed with the main helical gear (41), the main helical gear (41) is fixedly arranged at the output end of the first motor (412), the helical gear (412) is fixedly arranged in the first helical gear (410), and the helical gear (47), the first bevel gear (410) is in threaded connection with the second threaded part (48), the threaded rod (46) is located between the first threaded part (47) and the second threaded part (48), the first bevel gear (410) is rotatably mounted in a groove formed in a sliding block (49), a through hole (491) used for sliding on the first threaded part (47) and the second threaded part (48) is formed in the sliding block (49), and a strip-shaped protrusion (492) used for sliding in the groove formed in the circular mounting plate (11) is formed in the sliding block (49).
2. The device for detecting RNA in exosome according to claim 1, wherein the agitating mechanism (3) comprises a second motor (31), a main gear (32), an agitating wheel (33) and an agitating rod (34), the second motor (31) is fixedly installed on the circular installation plate (11), the main gear (32) is fixedly installed at the output end of the second motor (31), the agitating wheel (33) is provided with insections (331) used for being meshed with the main gear (32), the agitating rod (34) is fixedly installed on the lower end face of the agitating wheel (33), and the lower end face of the agitating rod (34) abuts against the upper end face of the rubber pad (5);
the stirring rod (34) comprises a fixing rod (341), an internal spring (342) and a telescopic rod (343), the fixing rod (341) is fixedly installed on the lower end face of the stirring wheel (33), the fixing rod (341) is slidably installed in a groove formed in the telescopic rod (343), the internal spring (342) used for supporting the fixing rod (341) is arranged in the groove of the telescopic rod (343), and the lower end portion of the telescopic rod (343) abuts against the upper end face of the rubber pad (5).
3. The device for detecting RNA in exosome according to claim 1, wherein the bottom of the beaker (2) is provided with an output tube (21) for excretion, and the lower end of the rubber pad (5) is provided with a rubber bulge (51) for sealing the output tube (21).
4. The device for detecting RNA in exosome according to claim 3, further comprising a lifting mechanism (6), wherein the upper end of the lifting mechanism (6) is mounted on the mounting frame (1), and the lower end of the lifting mechanism (6) is mounted on the rubber pad (5).
5. The device for detecting RNA in exosome according to claim 4, wherein the lifting mechanism (6) comprises a motor III (61), an output gear (62), a gear ring (63), a lifting threaded rod (64), an upper hinge (65), a lower hinge (66) and a bottom rod (67), the motor III (61) is fixedly installed in a through hole formed in the mounting frame (1), the output end of the motor III (61) is fixedly installed with the output gear (62) for driving the gear ring (63), the gear ring (63) is in threaded connection with the lifting threaded rod (64), the upper hinge (65) hinged to the lower hinge (66) is rotatably installed in a groove formed in the bottom rod (67), the bottom rod (67) is fixedly installed in a groove formed in the rubber pad (5), and a through hole for the lifting threaded rod (64) to pass through is formed in the stirring wheel (33).
6. The device for detecting RNA in exosome according to claim 1, which is characterized by further comprising a convenient constant volume mechanism (7), wherein the convenient constant volume mechanism (7) is fixedly installed on the circular installation plate (11).
7. The device for detecting RNA in exosome according to claim 6, wherein the convenient constant volume mechanism (7) comprises an injection pipe (71), a hydraulic cylinder (72), a control ring (73), an elastic sheet (74), a spring (75) and a rubber tube (76), the injection pipe (71) is fixedly installed in a through hole formed in the circular mounting plate (11), the hydraulic cylinder (72) is fixedly installed in the through hole formed in the circular mounting plate (11), the elastic sheet (74) is fixedly installed on the lower end face of the circular mounting plate (11), the control ring (73) is fixedly installed on the output end of the hydraulic cylinder (72), the injection pipe (71) and the elastic sheet (74) are placed in the ring of the control ring (73), the elastic sheet (74) is connected with the injection pipe (71) through the spring (75), the rubber tube (76) is fixedly installed on the injection pipe (71), the rubber tube (76) is provided with an extension section (761), and the lower end of the elastic sheet (74) is in contact with the extension section (761).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211267631.0A CN115343131B (en) | 2022-10-17 | 2022-10-17 | RNA detection device in exosome based on tumor diagnosis and prediction |
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| CN202211267631.0A CN115343131B (en) | 2022-10-17 | 2022-10-17 | RNA detection device in exosome based on tumor diagnosis and prediction |
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| CN115343131B CN115343131B (en) | 2022-12-13 |
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| CN107551902A (en) * | 2017-09-07 | 2018-01-09 | 重庆开奇科技发展有限公司 | A kind of blood agitating device |
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| CN112246140A (en) * | 2020-10-12 | 2021-01-22 | 广州爱索达生物医药技术有限公司 | RNA detection device in exosome based on tumor diagnosis and prediction |
| CN216093430U (en) * | 2021-07-28 | 2022-03-22 | 邳州市杰承生物科技有限公司 | Edible mushroom raw material mixer |
| CN114588809A (en) * | 2021-12-27 | 2022-06-07 | 杭州康大晨星医学科技有限公司 | Stirring device for reagent emulsification |
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2022
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0838870A (en) * | 1994-07-29 | 1996-02-13 | Tokai Kikai Seisakusho:Kk | Stirring device |
| US20050258285A1 (en) * | 2004-05-20 | 2005-11-24 | Mccambridge James E | Method and apparatus for comminution of biological specimens |
| CN107551902A (en) * | 2017-09-07 | 2018-01-09 | 重庆开奇科技发展有限公司 | A kind of blood agitating device |
| CN210814899U (en) * | 2019-10-15 | 2020-06-23 | 德清亚泰肠衣有限公司 | Salt solution agitator tank of heparin sodium crude product |
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| CN216093430U (en) * | 2021-07-28 | 2022-03-22 | 邳州市杰承生物科技有限公司 | Edible mushroom raw material mixer |
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| CN115343131B (en) | 2022-12-13 |
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