CN112246140A - RNA detection device in exosome based on tumor diagnosis and prediction - Google Patents
RNA detection device in exosome based on tumor diagnosis and prediction Download PDFInfo
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- CN112246140A CN112246140A CN202011081547.0A CN202011081547A CN112246140A CN 112246140 A CN112246140 A CN 112246140A CN 202011081547 A CN202011081547 A CN 202011081547A CN 112246140 A CN112246140 A CN 112246140A
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- 238000001514 detection method Methods 0.000 title claims abstract description 89
- 210000001808 exosome Anatomy 0.000 title claims abstract description 13
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 9
- 238000003745 diagnosis Methods 0.000 title claims abstract description 9
- 230000003472 neutralizing effect Effects 0.000 claims abstract description 68
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 238000003756 stirring Methods 0.000 claims abstract description 24
- 239000013614 RNA sample Substances 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000006386 neutralization reaction Methods 0.000 claims description 24
- 230000005540 biological transmission Effects 0.000 claims description 18
- 239000011521 glass Substances 0.000 claims description 11
- 238000003825 pressing Methods 0.000 claims description 10
- 238000007599 discharging Methods 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 10
- 230000000903 blocking effect Effects 0.000 description 5
- 238000007789 sealing Methods 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000010365 information processing Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009461 vacuum packaging Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/93—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with rotary discs
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
- B01F35/71805—Feed mechanisms characterised by the means for feeding the components to the mixer using valves, gates, orifices or openings
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/75—Discharge mechanisms
- B01F35/754—Discharge mechanisms characterised by the means for discharging the components from the mixer
- B01F35/7547—Discharge mechanisms characterised by the means for discharging the components from the mixer using valves, gates, orifices or openings
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
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Abstract
The invention discloses a tumor diagnosis and prediction-based RNA detection device in exosomes, which comprises a power body, a detection box and a neutralizing body, the detection box is fixedly arranged on the top surface of the power body close to the left side, the neutralizing body is fixedly arranged on the left side surface of the power body, the bottom surface of the neutralizing body is fixedly connected with the top surface of the detection box, a neutralizing mechanism capable of neutralizing and blending an RNA sample is arranged in the neutralizing body, the invention adopts a mode that the reciprocating mechanism adds RNA samples and neutralizing liquid into the neutralizing cavity at intervals to stir and mix, can greatly improve the neutralizing effect of the final detection liquid and ensure that the purple light detection effect of RNA is more accurate, meanwhile, only one motor is adopted, so that the moving block can be controlled to drive the detection die to move left and right to enter and exit the detection cavity.
Description
Technical Field
The invention relates to the technical field of detection correlation, in particular to a device for detecting RNA in exosomes based on tumor diagnosis and prediction.
Background
The tumor diagnosis and prediction generally detects whether RNA in exosomes is normal, an RNA sample is generally acidic, the condition of internal components can be detected more accurately only after neutralization is carried out, the purple light searchlighting detection and the observation of the illumination color change of a test sample are accurate and simple detection methods, and generally many complex operations are required to be manually processed, so that the operation is very troublesome and operation errors are easy to occur, and in addition, the RNA sample and neutralization liquid are required to be slowly and gradually added in the neutralization reaction, so that the detection accuracy can be ensured.
Disclosure of Invention
The invention aims to provide a device for detecting RNA in exosome based on tumor diagnosis and prediction, and solves the problems of low detection efficiency and low accuracy of a vacuum packaging bag.
The invention is realized by the following technical scheme.
The invention relates to an RNA detection device in exosome based on tumor diagnosis and prediction, which comprises a power body, a detection box and a neutralizing body, wherein the detection box is fixedly arranged on the top surface of the left side of the power body, the neutralizing body is fixedly arranged on the left side surface of the power body, the bottom surface of the neutralizing body is fixedly connected with the top surface of the detection box, a neutralizing mechanism capable of neutralizing and blending an RNA sample is arranged in the neutralizing body, the neutralizing mechanism comprises two solution tanks fixedly arranged on the top surface of the neutralizing body, a storage cavity with an upward opening is formed in each solution tank, the RNA sample is placed in the storage cavity on the left side, neutralizing liquid is placed in the storage cavity on the right side, a neutralizing cavity is formed in the neutralizing body, a connecting pipe is arranged on the lower side of the storage cavity in a communicating manner, stirring turntables are respectively arranged on the left side and the right side of the neutralizing cavity in a communicating manner, and the bottom end of the connecting pipe is communicated, the top wall of the neutralizing chamber is rotatably provided with a stirring turntable, the bottom surface of the stirring turntable is fixedly provided with a stirring rod, the upper side of the neutralizing chamber is provided with a cam chamber, a cam shaft is rotatably arranged between the upper wall and the lower wall of the cam chamber, the periphery of the cam shaft is fixedly provided with a cam, the left side and the right side of the cam chamber are communicated with an elastic chamber, the elastic chamber is communicated with two discharging chambers, the inner wall of the elastic chamber is provided with a sliding plate in a left-right sliding manner, the two sliding plates are mutually close to the side surface and are positioned in the cam chamber and abutted against the cam, the sliding plate can seal the discharging chambers, the sliding plate is internally provided with a through hole, the through hole can be communicated with the discharging chambers, a detection control mechanism which can receive the neutralized detection liquid and convey the detection liquid into the detection box is arranged between the neutralizing body and the power body, and the detection control mechanism comprises, the power body is internally provided with a moving cavity with an upward opening, the moving block is arranged on the bottom wall of the moving cavity in a left-right sliding manner, the moving block is internally provided with an installation cavity with an upward opening, the bottom wall of the installation cavity is placed and installed with a detection die, the detection die is internally provided with nine die cavities which are arranged in an array manner and have upward openings, the power body is internally provided with a Z-shaped cavity with a leftward opening, the bottom wall of the Z-shaped cavity is provided with a top pressing block in a left-right sliding manner, the extending part of the top pressing block can extend out of the outside and can be abutted against the right side surface of the moving block, the bottom surface of the neutralizing body is fixedly provided with a liquid dropping nozzle, the lower side of the neutralizing cavity is communicated with a through cavity, the liquid dropping nozzle is communicated with the through cavity, the right side of the through cavity is communicated with a control cavity with a downward, the left side intercommunication that leads to the chamber is equipped with the sector chamber, the roof horizontal slip in sector chamber is equipped with blocks the piece, block the piece and can insert lead to the intracavity and seal lead to the chamber, the roof in sector chamber rotates and is equipped with the vertical axis, the periphery of vertical axis has set firmly the sector wheel, the sector wheel can with block the piece butt.
Preferably, a motor is fixedly installed on the bottom wall of the Z-shaped cavity, a controller is electrically connected to the left side surface of the motor, a contact block is arranged on the right wall of the Z-shaped cavity in a vertically sliding manner, the contact block and the controller can control the motor to rotate reversely, a connecting rod is hinged between the contact block and the top pressing block, a reset spring is fixedly installed between the right side surface of the control bevel gear set and the right wall of the Z-shaped cavity, a top gear cavity is formed in the upper side of the Z-shaped cavity, a bottom gear cavity is formed in the lower side of the Z-shaped cavity, a motor shaft is dynamically connected to the upper side surface and the lower side surface of the motor, two motor shaft extending parts are respectively located in the top gear cavity and the bottom gear cavity, a gear shaft is rotatably arranged on the rear wall of the top gear cavity, and a transmission bevel gear set, the left wall of the bottom gear cavity is rotatably provided with a screw, a control bevel gear set is arranged between the motor shaft and the screw at the lower side in a transmission connection mode, the left extending portion of the screw penetrates through the inner walls of the power body and the moving block and is rotatably connected with the left wall of the moving cavity, and the inner wall of the moving block is in threaded connection with the screw.
Preferably, two bilaterally symmetrical square cavities are formed in the neutralizing body, the upper extending part of the cam shaft is positioned in the right square cavity, the rear walls of the two square cavities are both provided with driven shafts in a rotating manner, a meshing bevel gear set is arranged between the driven shaft positioned at the right side and the cam shaft in a transmission connection manner, a telescopic spring is fixedly arranged between the side surface of the sliding plate away from the cam and the inner wall of the elastic cavity, the lower extending part of the cam shaft penetrates through the inner wall of the neutralizing body and is fixedly connected with the top surface of the stirring turntable, the upper extending part of the vertical shaft is positioned in the left square cavity and is provided with the meshing bevel gear set in a transmission connection manner with the driven shaft positioned at the left side, a worm wheel cavity is formed in the neutralizing body and is positioned at the rear side of the square cavity, and the backward extending parts of the two driven shafts, and all set firmly the worm wheel in its periphery, it connects the chamber to have seted up in the power body, it is located to connect the chamber top gear chamber rear side, connect the chamber with the transmission is equipped with the worm between the inner wall in worm wheel chamber, is located in the worm wheel intracavity the worm with the worm wheel meshing, gear shaft rear side extension is located connect the intracavity, the gear shaft with the transmission is connected between the worm and is equipped with driven bevel gear group, the detachable closing cap is installed to the top surface of solution tank, block the right flank of piece with fixed mounting has connecting spring between the right wall in sector wheel chamber.
Preferably, a movable rack is fixedly embedded in the inner wall of the lower side of the control block, a bearing seat is fixedly arranged on the bottom surface of the neutralizing body, a rotating shaft is arranged in the bearing seat in a rotating mode, a rotating gear is fixedly arranged on the rear side face of the rotating shaft, the extending portion of the upper side of the rotating gear is located in the control cavity and meshed with the movable rack, a one-way threading die rack is fixedly arranged on the top surface of the movable block and can be meshed with the rotating gear, and a jacking spring is fixedly arranged between the right side face of the control block and the right wall of the Z-shaped cavity.
Preferably, be equipped with the detection chamber that the opening is right in the detection case, the movable block with the detection mould can get into detect the intracavity, the roof fixed mounting that detects the chamber has the purple light lamp, the left wall fixed mounting that detects the chamber has the detection camera, it has the processing display screen to inlay in the upside inner wall of detection case admittedly, the processing display screen with through signal layer electric connection between the detection camera, set firmly two symmetrical glass pieces around on the top surface of the power body, the glass piece with detection case fixed connection, the glass piece with neutralization body fixed connection.
The invention has the beneficial effects that: according to the invention, the RNA sample and the neutralization liquid are added into the neutralization cavity at intervals by the reciprocating mechanism to stir and mix, so that the neutralization effect of the final detection liquid can be greatly improved, the purple light detection effect of RNA is more accurate, meanwhile, only one motor is adopted, the moving block can be controlled to drive the detection die to move left and right to enter and exit the detection cavity, and the detection liquid after neutralization is added into the die cavity when the moving block moves left, so that the whole operation is more intelligent, manual operation is not needed, the structural design in the device is ingenious, and the device has a good linkage effect.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic view of the internal structure of the worm cavity and connecting cavity of the present invention;
FIG. 3 is a cross-sectional view taken along the line A-A of FIG. 1 according to an embodiment of the present invention
FIG. 4 is an enlarged schematic view of the embodiment of the present invention at B in FIG. 1.
Detailed Description
The invention will now be described in detail with reference to fig. 1-4, wherein for ease of description the orientations described hereinafter are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
The device for detecting RNA in exosome based on tumor diagnosis and prediction as shown in the attached drawings 1-4 comprises a power body 20, a detection box 49 and a neutralizing body 15, wherein the detection box 49 is fixedly arranged on the top surface of the power body 20 close to the left side, the neutralizing body 15 is fixedly arranged on the left side surface of the power body 20, the bottom surface of the neutralizing body 15 is fixedly connected with the top surface of the detection box 49, a neutralizing mechanism 101 capable of neutralizing and blending an RNA sample is arranged in the neutralizing body 15, the neutralizing mechanism 101 comprises two solution tanks 12 fixedly arranged on the top surface of the neutralizing body 15, storage cavities 13 with upward openings are arranged in the solution tanks 12, the RNA sample is arranged in the storage cavity 13 on the left side, a neutralizing liquid is arranged in the storage cavity 13 on the right side, a neutralizing cavity 56 is arranged in the neutralizing body 15, connecting pipes 14 are arranged on the lower sides of the storage cavities 13 in a communicating manner, the left side and the right side of the neutralization cavity 56 are both communicated with stirring turntables 58, the bottom end of the connecting pipe 14 is communicated with the stirring turntables 58, the top wall of the neutralization cavity 56 is rotatably provided with the stirring turntables 58, the bottom surface of the stirring turntables 58 is fixedly provided with stirring rods 57, the upper side of the neutralization cavity 56 is provided with cam cavities 71, the upper wall and the lower wall of the cam cavities 71 are rotatably provided with cam shafts 72, the periphery of the cam shafts 72 is fixedly provided with cams 73, the left side and the right side of the cam cavities 71 are communicated with elastic cavities 67, the elastic cavities 67 are communicated with the two discharging cavities 59, the inner walls of the elastic cavities 67 are provided with sliding plates 70 in a left-right sliding manner, the two sliding plates 70 are close to each other and positioned in the cam cavities 71 and abutted against the cams 73, the sliding plates 70 can seal the discharging cavities 59, through holes 69 are arranged in the sliding plates 70, and the through holes 69 can be communicated, a detection control mechanism 102 which can receive neutralized detection liquid and convey the detection liquid into a detection box 49 is arranged between the neutralization body 15 and the power body 20, the detection control mechanism 102 comprises a moving block 33, a moving cavity 46 with an upward opening is arranged in the power body 20, the moving block 33 is arranged on the bottom wall of the moving cavity 46 in a left-right sliding manner, an installation cavity 34 with an upward opening is arranged in the moving block 33, a detection mold 35 is placed and installed on the bottom wall of the installation cavity 34, nine mold cavities 36 which are arranged in an array manner and have upward openings are arranged in the detection mold 35, a Z-shaped cavity 22 with a leftward opening is arranged in the power body 20, a top pressing block 28 is arranged on the bottom wall of the Z-shaped cavity 22 in a left-right sliding manner, the extending part of the top pressing block 28 can extend out of the outside and can be abutted against the right side surface of the moving block 33, and a liquid dropping nozzle 42 is fixedly installed on the, the downside intercommunication in neutralization chamber 56 is equipped with logical chamber 43, dropping liquid shower nozzle 42 with logical chamber 43 intercommunication, the right side intercommunication that leads to chamber 43 is equipped with the control chamber 21 that the opening is decurrent, the roof horizontal slip of control chamber 21 is equipped with control block 41, control block 41 can insert lead to the intracavity 43 internal closure lead to chamber 43, the left side intercommunication that leads to chamber 43 is equipped with sector wheel chamber 53, the roof horizontal slip of sector wheel chamber 53 is equipped with blocks piece 44, block piece 44 and insert lead to the intracavity 43 internal closure lead to chamber 43, the roof rotation in sector wheel chamber 53 is equipped with vertical axis 55, the periphery of vertical axis 55 has set firmly sector wheel 54, sector wheel 54 can with block piece 44 butt.
Beneficially, a motor 23 is fixedly mounted on the bottom wall of the Z-shaped cavity 22, a controller 24 is electrically connected to the left side surface of the motor 23, a contact block 25 is slidably disposed on the right wall of the Z-shaped cavity 22 up and down, the contact block 25 and the controller 24 can control the motor 23 to rotate reversely, a connecting rod 26 is hinged between the contact block 25 and the top press block 28, a return spring 27 is fixedly mounted between the right side surface of the control bevel gear set 29 and the right wall of the Z-shaped cavity 22, a top gear cavity 16 is formed in the upper side of the Z-shaped cavity 22, a bottom gear cavity 30 is formed in the lower side of the Z-shaped cavity 22, a motor shaft 19 is dynamically connected to both the upper side surface and the lower side surface of the motor 23, the extending portions of the two motor shafts 19 are respectively located in the top gear cavity 16 and the bottom gear cavity 30, and a gear shaft 17, a transmission bevel gear set 18 is arranged between the gear shaft 17 and the motor shaft 19 positioned at the upper side in a transmission connection mode, a screw 31 is arranged on the left wall of the bottom gear cavity 30 in a rotating mode, a control bevel gear set 29 is arranged between the motor shaft 19 and the screw 31 positioned at the lower side in a transmission connection mode, the left extending portion of the screw 31 penetrates through the inner walls of the power body 20 and the moving block 33 and is connected with the left wall of the moving cavity 46 in a rotating mode, the inner wall of the moving block 33 is in threaded connection with the screw 31, the motor shaft 19 can be controlled to drive the gear shaft 17 and the screw 31 to rotate forwardly through the forward operation of the motor 23, the moving block 33 can be controlled to move rightwards through the forward rotation of the screw 31, when the moving block 33 moves to the rightmost side, the moving block 33 can push the top pressing block 28 to move rightwards, the contact block 25 can be brought into contact with the controller 24, and then the controller 24 can control the motor 23 to run in reverse, so that the motor shaft 19 can drive the gear shaft 17 and the screw 31 to rotate in reverse, and the screw 31 rotates in reverse to move the moving block 33 to the left again.
Advantageously, two bilaterally symmetrical square cavities 74 are formed in the neutralizing body 15, the upper extension part of the cam shaft 72 is positioned in the right square cavity 74, the rear walls of the two square cavities 74 are respectively provided with a driven shaft 61 in a rotating manner, a meshing bevel gear set 75 is arranged between the driven shaft 61 and the cam shaft 72 on the right side in a transmission connection manner, a telescopic spring 68 is fixedly arranged between the side surface of the sliding plate 70 away from the cam 73 and the inner wall of the elastic cavity 67, the lower extension part of the cam shaft 72 penetrates through the inner wall of the neutralizing body 15 and is fixedly connected with the top surface of the stirring turntable 58, the upper extension part of the vertical shaft 55 is positioned in the left square cavity 74 and is provided with the meshing bevel gear set 75 in a transmission connection manner between the driven shaft 61 on the left side, a worm wheel cavity 60 is formed in the neutralizing body 15, the worm wheel cavity 60 is positioned on the rear side of the square cavity 74, the backward extending parts of the two driven shafts 61 extend into the worm wheel cavity 60, worm wheels 62 are fixedly arranged on the peripheries of the two driven shafts, a connecting cavity 64 is formed in the power body 20, the connecting cavity 64 is positioned at the back side of the top gear cavity 16, a worm 63 is arranged between the connecting cavity 64 and the inner wall of the worm wheel cavity 60 in a transmission manner, the worm 63 positioned in the worm wheel cavity 60 is meshed with the worm wheels 62, the backward extending part of the gear shaft 17 is positioned in the connecting cavity 64, a driven bevel gear set 65 is arranged between the gear shaft 17 and the worm 63 in a transmission connection manner, a detachable sealing cover 11 is arranged on the top surface of the solution tank 12, a connecting spring 45 is fixedly arranged between the right side surface of the blocking block 44 and the right wall of the fan wheel cavity 53, the RNA sample and the neutralizing liquid can be added into the storage cavity 13 by detaching and opening the sealing cover 11, when the gear shaft 17 rotates positively, the worm 63 can drive the driven shaft 61 to rotate positively, so that the cam shaft 72 and the vertical shaft 55 rotate positively, at the moment, the sector wheel 54 can be driven to drive the blocking block 44 to move back and forth left and right, the lower side part of the through cavity 43 can be intermittently in a communicated state, meanwhile, the cam shaft 72 can drive the cam 73 to rotate, the cam 73 can drive the two sliding plates 70 to move back and forth and left and right, and then the RNA sample and the neutralization solution can be gradually added into the neutralization cavity 56 in batches, so that the neutralization effect can be improved, and meanwhile, the cam shaft 72 can drive the stirring turntable 58 and the stirring rod 57 to rotate, so that the stirring rod 57 can achieve the effect, and the neutralization effect can be improved.
Beneficially, a moving rack 40 is fixedly embedded in an inner wall of a lower side of the control block 41, a bearing seat 37 is fixedly arranged on a bottom surface of the neutralizing body 15, a rotating shaft 38 is rotatably arranged in the bearing seat 37, a rotating gear 39 is fixedly arranged on a rear side surface of the rotating shaft 38, an upper extending portion of the rotating gear 39 is positioned in the control cavity 21 and is meshed with the moving rack 40, a one-way threading die rack 32 is fixedly arranged on a top surface of the moving block 33, the one-way threading die rack 32 can be meshed with the rotating gear 39, a jacking spring 76 is fixedly arranged between a right side surface of the control block 41 and a right wall of the Z-shaped cavity 22, the control block 41 can be normally in a state of closing the through cavity 43 through the jacking spring 76, when the moving block 33 moves leftwards, the vertical shaft 55 rotates reversely, and the sector wheel 54 drives the blocking block 44 to move leftwards and right, meanwhile, the one-way die rack 32 can drive the rotating gear 39 to rotate clockwise, so that the control block 41 can be opened by moving rightward, and then the dripping nozzle 42 can be discharged into the die cavity 36 to neutralize the detection liquid in the neutralizing cavity 56, so that the die cavity 36 can be filled with the detection liquid.
Beneficially, a detection chamber 47 with a right opening is arranged in the detection box 49, the moving block 33 and the detection mold 35 can enter the detection chamber 47, a violet light irradiation lamp 48 is fixedly installed on the top wall of the detection chamber 47, a detection camera 50 is fixedly installed on the left wall of the detection chamber 47, a processing display screen 52 is fixedly embedded in the inner wall of the upper side of the detection box 49, the processing display screen 52 is electrically connected with the detection camera 50 through a signal layer 51, two glass blocks 66 which are symmetrical in the front-back direction are fixedly installed on the top surface of the power body 20, the glass blocks 66 are fixedly connected with the detection box 49, the glass blocks 66 are fixedly connected with the neutralizing body 15, when the moving block 33 moves leftwards to enter the detection chamber 47, the detection condition of the detection liquid in the mold chamber 36 can be detected by the detection camera 50 through the irradiation of the violet light irradiation lamp 48, the information processing can be fed back through the processing display screen 52, and the internal situation can be conveniently observed by the staff through the glass block 66.
In the initial state, the moving block 33 is located in the detection cavity 47, the control block 41 and the blocking block 44 close the through cavity 43, the convex part of the cam 73 faces backwards, the two sliding plates 70 both close the discharging cavity 59, the jacking block 28 extends out of the outside, and the contact block 25 is not in contact with the controller 24.
When the device is used, the motor shaft 19 can be controlled to drive the gear shaft 17 and the screw rod 31 to rotate forwards through the forward operation of the motor 23, the screw rod 31 can be controlled to rotate forwards to control the moving block 33 to move rightwards, when the moving block 33 moves to the rightmost side, the moving block 33 can be enabled to push the top pressing block 28 to move rightwards, so that the connecting rod 26 can drive the contact block 25 to ascend, at the moment, the contact block 25 can be enabled to be in contact with the controller 24, then the controller 24 can be enabled to control the motor 23 to rotate reversely, at the moment, the motor shaft 19 can drive the gear shaft 17 and the screw rod 31 to rotate reversely, the moving block 33 can be enabled to move leftwards again through the reverse rotation of the screw rod 31, the RNA sample and the neutralizing liquid can be added into the storage cavity 13 through disassembling and opening the sealing cover 11, when the gear shaft 17 rotates forwards, the worm 63 can drive the, the lower part of the through cavity 43 can be intermittently communicated, meanwhile, the cam shaft 72 can drive the cam 73 to rotate, the cam 73 can drive the two sliding plates 70 to move left and right back and forth, then the RNA sample and the neutralization solution can be gradually added into the neutralization cavity 56 in batches, then the neutralization effect can be improved, meanwhile, the cam shaft 72 can drive the stirring turntable 58 and the stirring rod 57 to rotate, then the stirring rod 57 can achieve the effect, the neutralization effect is improved, the control block 41 can be normally in the state of closing the through cavity 43 through the top pressure spring 76, when the moving block 33 moves left, the vertical shaft 55 rotates reversely, the sector wheel 54 drives the blocking block 44 to move left and right back and forth, meanwhile, the one-way die rack 32 can drive the rotating gear 39 to rotate clockwise, so that the control block 41 can move right and open, and then the liquid dropping nozzle 42 can discharge the detection solution which is neutralized and completed in the neutralization cavity 56 into the die cavity 36, the mold cavity 36 can be filled with the detection liquid, when the moving block 33 moves leftwards to enter the detection cavity 47, the detection condition of the detection liquid in the mold cavity 36 can be detected by the detection camera 50 through the irradiation of the purple light irradiation lamp 48, the information can be fed back through the processing display screen 52, and the internal condition can be conveniently observed by a worker through the glass block 66.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (5)
1. A RNA detection device in exosome based on tumor diagnosis and prediction comprises a dynamic body, a detection box and a neutralizing body, and is characterized in that: the detection box is fixedly arranged on the top surface of the power body close to the left side, the neutralizing body is fixedly arranged on the left side surface of the power body, the bottom surface of the neutralizing body is fixedly connected with the top surface of the detection box, a neutralizing mechanism capable of neutralizing and blending RNA samples is arranged in the neutralizing body, the neutralizing mechanism comprises two solution tanks fixedly arranged on the top surface of the neutralizing body, a storage cavity with an upward opening is formed in each solution tank, the RNA samples are placed in the storage cavities on the left side, neutralizing liquid is placed in the storage cavities on the right side, a neutralizing cavity is formed in the neutralizing body, a connecting pipe is communicated and installed at the lower side of the storage cavity, stirring turntables are communicated and arranged on the left side and the right side of the neutralizing cavity, the bottom end of the connecting pipe is communicated with the stirring turntables, stirring turntables are rotatably arranged on the top walls of the neutralizing cavities, and stirring rods are fixedly arranged on the, a cam cavity is formed in the upper side of the neutralization cavity, a cam shaft is rotatably arranged between the upper wall and the lower wall of the cam cavity, a cam is fixedly arranged on the periphery of the cam shaft, the left side and the right side of the cam cavity are communicated with elastic cavities, the elastic cavities are communicated with the two discharging cavities, sliding plates are arranged on the inner wall of each elastic cavity in a left-right sliding mode, the two sliding plates are close to each other, the side faces of the two sliding plates are located in the cam cavity and abut against the cam, the discharging cavities can be sealed by the sliding plates, through holes are formed in the sliding plates, and the through holes can be communicated with the discharging cavities;
a detection control mechanism which can receive neutralized detection liquid and convey the detection liquid into a detection box for detection is arranged between the neutralizing body and the power body, the detection control mechanism comprises a moving block, a moving cavity with an upward opening is arranged in the power body, the moving block is arranged on the bottom wall of the moving cavity in a left-right sliding manner, an installation cavity with an upward opening is arranged in the moving block, a detection mold is placed and installed on the bottom wall of the installation cavity, nine mold cavities which are arranged in an array manner and have upward openings are arranged in the detection mold, a Z-shaped cavity with a leftward opening is arranged in the power body, a top pressing block is arranged on the bottom wall of the Z-shaped cavity in a left-right sliding manner, the extending part of the top pressing block can extend out of the outside and can be abutted against the right side surface of the moving block, a liquid dropping nozzle is fixedly installed on the bottom surface of the neutralizing body, the dropping liquid shower nozzle with lead to the chamber intercommunication, the right side intercommunication that leads to the chamber is equipped with the control chamber that the opening is decurrent, the roof horizontal slip in control chamber is equipped with the control block, the control block can insert lead to the intracavity and seal lead to the chamber, the left side intercommunication that leads to the chamber is equipped with the sector wheel chamber, the roof horizontal slip in sector wheel chamber is equipped with blocks the piece, it can insert to block the piece lead to the intracavity and seal lead to the chamber, the roof in sector wheel chamber rotates and is equipped with the vertical axis, the periphery of vertical axis has set firmly the sector wheel, the sector wheel can with block the piece butt.
2. The device for detecting RNA in exosome according to claim 1, wherein: a motor is fixedly installed on the bottom wall of the Z-shaped cavity, a controller is electrically connected with the left side surface of the motor, a contact block is arranged on the right wall of the Z-shaped cavity in a vertically sliding mode, the contact block and the controller can control the motor to rotate reversely, a connecting rod is hinged between the contact block and the top pressing block, a reset spring is fixedly installed between the right side surface of the control bevel gear set and the right wall of the Z-shaped cavity, a top gear cavity is formed in the upper side of the Z-shaped cavity, a bottom gear cavity is formed in the lower side of the Z-shaped cavity, the upper side surface and the lower side surface of the motor are both provided with a motor shaft in a power connection mode, two motor shaft extending parts are respectively located in the top gear cavity and the bottom gear cavity, a gear shaft is rotatably arranged on the rear wall of the top gear cavity, the left wall of the bottom gear cavity is rotatably provided with a screw, a control bevel gear set is arranged between the motor shaft and the screw at the lower side in a transmission connection mode, the left extending portion of the screw penetrates through the inner walls of the power body and the moving block and is rotatably connected with the left wall of the moving cavity, and the inner wall of the moving block is in threaded connection with the screw.
3. The device for detecting RNA in exosome according to claim 2, wherein: the neutralizing body is internally provided with two bilaterally symmetrical square cavities, the upper extension part of the cam shaft is positioned in the right square cavity, the rear walls of the two square cavities are both provided with driven shafts in a rotating way, a meshing bevel gear set is arranged between the driven shaft positioned on the right side and the cam shaft in a transmission connection way, the sliding plate is far away from the side surface of the cam and the inner wall of the elastic cavity, the lower extension part of the cam shaft penetrates through the inner wall of the neutralizing body and is fixedly connected with the top surface of the stirring turntable, the upper extension part of the vertical shaft is positioned in the left square cavity and is provided with the meshing bevel gear set in a transmission connection way with the driven shaft positioned on the left side, a worm wheel cavity is arranged in the neutralizing body and is positioned on the rear side of the square cavity, and the backward extension parts of the two driven shafts extend into the, and all set firmly the worm wheel in its periphery, it connects the chamber to have seted up in the power body, it is located to connect the chamber top gear chamber rear side, connect the chamber with the transmission is equipped with the worm between the inner wall in worm wheel chamber, is located in the worm wheel intracavity the worm with the worm wheel meshing, gear shaft rear side extension is located connect the intracavity, the gear shaft with the transmission is connected between the worm and is equipped with driven bevel gear group, the detachable closing cap is installed to the top surface of solution tank, block the right flank of piece with fixed mounting has connecting spring between the right wall in sector wheel chamber.
4. The device for detecting RNA in exosome according to claim 3, wherein: the inner wall of the lower side of the control block is fixedly embedded with a movable rack, the bottom surface of the neutralizing body is fixedly provided with a bearing seat, a rotating shaft is arranged in the bearing seat in a rotating mode, a rotating gear is fixedly arranged on the rear side face of the rotating shaft, the extending portion of the upper side of the rotating gear is located in the control cavity and meshed with the movable rack, the top surface of the movable block is fixedly provided with an unidirectional threading die rack, the unidirectional threading die rack can be meshed with the rotating gear, and a jacking spring is fixedly arranged between the right side face of the control block and the right wall of the Z-shaped cavity.
5. The device for detecting RNA in exosome according to claim 4, wherein: be equipped with the detection chamber of opening right in the detection case, the movable block with it can get into to detect the mould detect the intracavity, the roof fixed mounting that detects the chamber has the purple light lamp, the left wall fixed mounting that detects the chamber has the detection camera, it has the processing display screen to inlay in the upside inner wall of detection case admittedly, the processing display screen with through signal layer electric connection between the detection camera, the glass piece of two front and back symmetries has set firmly on the top surface of the power body, the glass piece with detection case fixed connection, the glass piece with the body fixed connection of neutralization.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115343131A (en) * | 2022-10-17 | 2022-11-15 | 湖南慧登生物科技有限公司 | RNA detection device in exosome based on tumor diagnosis and prediction |
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| EP2063969A1 (en) * | 2006-09-19 | 2009-06-03 | Fuji Manufacturing Europe B.V. | Process and device for the precipitation of an organic compound |
| CN104379762A (en) * | 2012-02-03 | 2015-02-25 | 皮罗比特私人有限公司 | Rotatable platform for conducting nucleic acid sequencing |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2063969A1 (en) * | 2006-09-19 | 2009-06-03 | Fuji Manufacturing Europe B.V. | Process and device for the precipitation of an organic compound |
| CN104379762A (en) * | 2012-02-03 | 2015-02-25 | 皮罗比特私人有限公司 | Rotatable platform for conducting nucleic acid sequencing |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115343131A (en) * | 2022-10-17 | 2022-11-15 | 湖南慧登生物科技有限公司 | RNA detection device in exosome based on tumor diagnosis and prediction |
| CN115343131B (en) * | 2022-10-17 | 2022-12-13 | 湖南慧登生物科技有限公司 | RNA detection device in exosome based on tumor diagnosis and prediction |
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