CN115341001B - CO (carbon monoxide) 2 Method for regulating and controlling production of medium-chain fatty acid - Google Patents
CO (carbon monoxide) 2 Method for regulating and controlling production of medium-chain fatty acid Download PDFInfo
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- 150000004667 medium chain fatty acids Chemical class 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 45
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 27
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 26
- 230000001276 controlling effect Effects 0.000 title claims abstract description 19
- 229910002091 carbon monoxide Inorganic materials 0.000 title claims description 9
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 title claims description 4
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- 244000005700 microbiome Species 0.000 claims abstract description 25
- 238000006243 chemical reaction Methods 0.000 claims abstract description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000002243 precursor Substances 0.000 claims abstract description 11
- 239000000758 substrate Substances 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 102
- 239000010802 sludge Substances 0.000 claims description 37
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- 239000007787 solid Substances 0.000 claims description 19
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- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
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- 239000004310 lactic acid Substances 0.000 claims description 7
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- HNFOAHXBHLWKNF-UHFFFAOYSA-M sodium;2-bromoethanesulfonate Chemical compound [Na+].[O-]S(=O)(=O)CCBr HNFOAHXBHLWKNF-UHFFFAOYSA-M 0.000 claims description 6
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- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims description 4
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- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
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- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 25
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- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 8
- 238000007254 oxidation reaction Methods 0.000 description 8
- 230000003647 oxidation Effects 0.000 description 7
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 6
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- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 4
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- AWQSAIIDOMEEOD-UHFFFAOYSA-N 5,5-Dimethyl-4-(3-oxobutyl)dihydro-2(3H)-furanone Chemical compound CC(=O)CCC1CC(=O)OC1(C)C AWQSAIIDOMEEOD-UHFFFAOYSA-N 0.000 description 1
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- 108020005199 Dehydrogenases Proteins 0.000 description 1
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- GONOPSZTUGRENK-UHFFFAOYSA-N benzyl(trichloro)silane Chemical compound Cl[Si](Cl)(Cl)CC1=CC=CC=C1 GONOPSZTUGRENK-UHFFFAOYSA-N 0.000 description 1
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- 238000012163 sequencing technique Methods 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 235000021391 short chain fatty acids Nutrition 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 125000005480 straight-chain fatty acid group Chemical group 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
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- 238000010792 warming Methods 0.000 description 1
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
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Abstract
The invention provides a CO 2 Regulating and controlling production of medium chain fatA method of acid comprising: inoculating microorganism capable of performing carbon chain extension reaction to produce medium chain fatty acid by using electron donor and electron acceptor or electron acceptor precursor as substrate, and introducing CO into reactor 2 And controlling the concentration within a certain range to prepare the medium chain fatty acid. The invention adopts common electron donor and electron acceptor as fermentation substrate, and controls CO in the system in the presence of microorganism 2 The content can realize the regulation and control of the yield of the medium-chain fatty acid. And CO 2 As a common gas and a greenhouse gas with great influence on the environment, the source of the gas is wide, and the gas is formed by CO 2 The medium-chain fatty acid is regulated and controlled, waste is changed into valuable, the yield of the medium-chain fatty acid is greatly improved, the process is simple, and the method is economical and environment-friendly.
Description
Technical Field
The invention relates to the technical field of environmental engineering, in particular to a CO 2 Methods for modulating production of medium chain fatty acids.
Background
Since the industrialization, the demand for energy by human society has increased with the development of society and the increase of population. The energy which people depend on for production and life at present is still mainly fossil energy, and the non-renewable property of the energy leads to the fact that the energy is gradually consumed and finally causes serious energy shortage. In addition, fossil energy sources are developed and used and also cause serious environmental hazard, and a great deal of harmful gases are discharged (such as SO 2 ，CO 2 CO and smoke, etc.). These gases accumulate in large amounts in the environment, most likely destroying the ozone layer, causing global warming, inducing acid rain, and possibly increasing the frequency of natural disasters.
In order to alleviate the dependence on fossil energy, how to develop renewable energy sources and renewable chemicals is a current problem in the society that is urgently needed to be solved. The production of Medium Chain Fatty Acids (MCFAs) using carbon chain elongation techniques is a new approach to solve this problem. Short chain fatty acids produced by fermentation of organic substrates can be converted to medium chain fatty acids (C6-C12) via a carbon chain elongation process by electron donors (ethanol, lactic acid, etc.). Medium chain fatty acids, because of their longer carbon chains, have a higher energy density and a stronger hydrophobicity, and can therefore be separated from the fermentation system by a subsequent extraction process. Medium chain fatty acids are also a high value added product that can be used not only directly as an antimicrobial and food additive, but also further processed into liquid biofuels, including diesel and aviation fuels. However, despite the wide range of applications of medium chain fatty acids, the cost of producing it is currently quite expensive. Medium chain fatty acids are typically derived from vegetable or animal oils, petroleum, and therefore the use of common electron donors and acceptors for medium chain fatty acid production is an economical and environmentally friendly option.
In the study of the carbon chain extension process, the yield of medium chain fatty acids has been an important point of interest. The current research on control factors in the production process of medium-chain fatty acid is mainly based on the pH value of environmental factors, whether product extraction equipment is installed or not, and the control of methanogens in the reaction process. Electron transfer, as an important part of energy transfer, greatly affects product formation and is discussed in little literature. Thus, the CO in the production process is studied 2 And in particular to the electron transfer, has an important role in promoting the development of new technologies of interest.
Disclosure of Invention
Aiming at the problems and defects existing in the prior art, the invention provides a CO 2 Method for regulating and controlling production of medium-chain fatty acid by CO 2 The concentration is controlled within a reasonable range, so that the electron donor can be fully utilized, and the medium chain fatty acid with higher yield is obtained. The technical scheme of the invention is as follows:
CO (carbon monoxide) 2 A method of modulating production of medium chain fatty acids comprising: inoculating microorganism capable of performing carbon chain extension reaction to produce medium chain fatty acid by using electron donor and electron acceptor or electron acceptor precursor as substrate, and introducing CO into reactor 2 And controlling the concentration within a certain range to prepare the medium chain fatty acid.
Further, the method comprises the steps of:
step 1, mixing an electron donor and an electron acceptor or an electron acceptor precursor, further adding the microorganisms, uniformly mixing, and adjusting the pH of a system to 5-7;
Further, the electron donor is ethanol and/or lactic acid.
Preferably, the electron donor is ethanol.
Further, the electron acceptor is at least one of acetic acid, formic acid, propionic acid, butyric acid, valeric acid, succinic acid and malic acid.
Preferably, the electron acceptor is acetic acid.
Further, the electron acceptor precursor is selected from at least one of municipal sludge, surplus sludge, organic solid waste, and organic sewage.
Further, the microorganism is derived from at least one of activated sludge, anaerobic sludge, municipal sludge, pure bacteria, and a pure bacteria composition.
Further, the pure bacteria include bacteria of the Firmicum, proteobacteria.
Preferably, the pH of the system in the step 1 is adjusted to 5-6.
Preferably, the CO 2 CO in the mixture with nitrogen 2 The gas phase content is 10% -50%.
More preferably, the CO 2 CO in the mixture with nitrogen 2 The gas phase content was 30%.
Further, the reaction control conditions in the step 2 are as follows: aiming at an organic wastewater treatment system, the temperature is 28-40 ℃, the hydraulic retention time in the system is 2-20 d, the microorganism retention time is 5-20 d, and the stirring speed is 50-200 rpm; aiming at a solid waste treatment system, the temperature is 28-40 ℃, the solid residence time and the microorganism residence time in the system are 5-20 d, and the stirring speed is 50-200 rpm.
Further, after the microorganism is added in the step 1, sodium 2-bromoethyl sulfonate is further added.
Further, when sodium 2-bromoethyl sulfonate is added, the reaction in step 2 is controlled as follows: aiming at an organic wastewater treatment system, the temperature is 28-40 ℃, the hydraulic retention time in the system is 2-20 d, the microorganism retention time is 5-40 d, and the stirring speed is 50-200 rpm; aiming at a solid waste treatment system, the temperature is 28-40 ℃, and the solid residence time and the microorganism residence time are 5-40 d; the stirring speed is 50-200 rpm.
Further, the medium chain fatty acid is a straight chain fatty acid or a branched chain fatty acid with a carbon chain number of C6-C12.
The invention adopts common electron donor and electron acceptor or electron acceptor precursor as fermentation substrate, and controls CO in the system in the presence of microorganism 2 The content can realize the regulation and control of the yield of the medium-chain fatty acid. And CO 2 As a common gas and a greenhouse gas with great influence on the environment, the source of the gas is wide, and the gas is formed by CO 2 The medium-chain fatty acid is regulated and controlled, waste is changed into valuable, the yield of the medium-chain fatty acid is greatly improved, the process is simple, and the method is economical and environment-friendly.
Drawings
FIG. 1 shows various COs investigated in accordance with the invention 2 Activity in dehydrogenase-mediated electron transfer at concentration conditions.
FIG. 2 shows the different COs in example 1 of the present invention 2 Production of medium chain fatty acids at concentration is schematically shown.
FIG. 3 is a graph showing the amount of ethanol and the amount of electron transfer for the CE process and the ethanol oxidation process, respectively, in example 2 of the present invention, wherein the graph (a) is a graph showing the amount of ethanol for the CE process and the ethanol oxidation process, respectively; and (b) is a data graph of carbon chain extension electron transfer amount.
FIG. 4 shows the CO at different temperatures in example 2 of the present invention 2 Production of medium chain fatty acids at concentration is schematically shown.
FIG. 5 shows the CO at different temperatures in example 3 of the present invention 2 Production of medium chain fatty acids at concentration is schematically shown.
Detailed Description
In the description of the present invention, it is to be noted that the specific conditions are not specified in the examples, and the description is performed under the conventional conditions or the conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
The invention provides a CO 2 Method for regulating and controlling production of medium-chain fatty acid, using electron donor and electron acceptor or electron acceptor precursor as substrate, inoculating microorganism capable of making carbon chain extension reaction to produce medium-chain fatty acid, and using CO in reactor 2 And controlling the concentration within a certain range to prepare the medium chain fatty acid. The method has simple technical process, easily obtained raw materials, changes waste into valuable, provides a brand new idea for producing the medium-chain fatty acid, and is a method which takes economy and environmental protection into account.
In the method of the invention, the electron donor is ethanol and/or lactic acid, the electron acceptor is at least one of acetic acid, formic acid, propionic acid, butyric acid, valeric acid, succinic acid and malic acid, the electron acceptor precursor comprises municipal sludge, residual sludge, organic solid waste, organic sewage and the like, and the microorganism is at least one of activated sludge, anaerobic sludge, municipal sludge, pure bacteria and pure bacteria composition.
When the microorganism is pure bacteria, the microorganism of the genus Clostridium and the genus Osciliibacter are preferably pure bacteria of the phylum Firmicum and the phylum Proteobacteria.
We have found in the study that CO 2 Within a certain concentration range, electron transfer processes mediated by dehydrogenases can be promoted. As shown in FIG. 1, different COs are added 2 Concentration (i.e. CO) 2 In CO 2 And N 2 Gas phase content in the mixed gas) for 10 days, the microorganism of the system has an electron transfer activity of 1.2.+ -. 0.14. Mu.g/min.mL (CO) 2 Concentration 0%) to 1.6.+ -. 0.16. Mu.g/min.mL (CO) 2 Concentration 10%), 1.8.+ -. 0.19. Mu.g/min.mL (CO) 2 Concentration of 20%) up to 2.1.+ -. 0.28. Mu.g/min.mL (CO) 2 Concentration 30%). Thereafter, with CO 2 The concentration was further increased, and the microbial electronic activity in the system was gradually decreased to 1.5.+ -. 0.15. Mu.g/min.mL (CO 2 Concentration 40%), 1.2.+ -. 0.12. Mu.g/min.mL (CO) 2 Degree of 50%), 0.88.+ -. 0.01. Mu.g/min.mL (CO) 2 Concentration 75%). It can be seen that the microbial electron transfer system activity in the system shows a trend of increasing and decreasing, and the highest electron transfer activity is 30% of CO 2 Detected at a concentration consistent with the highest medium chain fatty acid yield at 30%. During the formation of medium chain acids, such as the oxidation of ethanol to acetyl-CoA and the subsequent extension of the carbon chain, there is a dehydrogenase which is not involved in the oxidation of acetyl-CoA to acetic acid. Thus, with CO 2 The content gradually increased from 0% to 30% and the INT-electron transfer activity gradually increased, and the dehydrogenase activity gradually increased, indicating more electron transfer to the direction of carbon chain extension. But with CO 2 The content increased again from 30% to 75% and the INT-electron transfer activity decreased and the dehydrogenase activity decreased, indicating more electron transfer toward ethanol oxidation (FIG. 3 (a), section 2 below).
The invention will now be described in further detail with reference to the drawings and to specific examples, which are given by way of illustration and not limitation.
Example 1
The present embodiment provides a CO 2 Method for regulating and controlling production of medium-chain fatty acid and investigating different COs 2 The effect of the gas phase content is as follows:
ethanol with a final concentration of 4000mg/L (8360 mgCOD/L) and acetic acid with a final concentration of 800mg/L (856 mgCOD/L) are respectively used as an electron donor and an electron acceptor, 100g of anaerobic sludge is added, the Total Solid (TS) of the anaerobic sludge is 0.03g/g, the total volume of the Volatile Solid (VS) is 0.015g/g, the total volume of materials in the system is 1L, the pH in the reactor is adjusted to be 5.5, and further sodium 2-bromoethyl sulfonate is added, and different COs are used 2 Gas cylinders (0%, 10%, 20%, 30%, 40%, 50%, 75%) of the gas phase components purge the reactor headspace, different CO 2 The flow rate of the mixed gas is 1L/min, the purging time is 5min, and the CO in the reactor is regulated and controlled 2 The components substantially replace air while also maintaining an anaerobic environment within the reactor. After sealing the reactor, the reactor was adjustedThe temperature of the reactor is 35 ℃, the rotating speed is 170rpm, and the retention time of the hydraulic and anaerobic sludge in the system is 12d. Samples were taken every 2 days, and the concentration of medium chain fatty acid was measured by gas chromatography, and as shown in FIG. 2, after the waiting reaction was stabilized, it was found that a large amount of ethanol remained in the 0% group experiment, and the remaining ethanol reached 3828mg COD/L. In the 10% group experiments, it was also found that a small amount of ethanol had not been fully reacted, and the remaining ethanol reached 1584mg COD/L. The remaining group of ethanol can be considered to be fully reacted. The medium chain fatty acid produced by the reaction is mainly caproic acid, 0%, 10%, 20%, 30%, 40%, 50%,75% group CO 2 Caproic acid was produced at levels 955, 2619, 3058, 3079, 2033, 1871, 1469mg COD/L, respectively. Caproic acid content in CO 2 The concentration was highest at 30%, the ratio in the final product was 43.1%, followed by CO 2 The concentration was 20%.
Example 2
The present embodiment provides a CO 2 Method for regulating and controlling production of medium-chain fatty acid and investigating different COs 2 The effect of the gas phase content is as follows:
the final concentration of 750mg/L (1568 mgCOD/L) ethanol and the final concentration of 250mg/L (268 mgCOD/L) acetic acid are respectively used as electron donor and electron acceptor, 5g of anaerobic sludge is added, the Total Solid (TS) of the anaerobic sludge is 0.027g/g, the Volatile Solid (VS) is 0.015g/g, the total volume of materials in the system is 0.05L, the pH in the reactor is regulated to be 5.0, and different COs are used 2 Gas cylinders (0%, 10%, 20%, 30%, 40%, 50%, 75%) of the gas phase components purge the reactor headspace, different CO 2 The flow rate of the mixed gas is 0.05L/min, the purging time is 5min, and the CO in the reactor is regulated and controlled 2 The components substantially replace air while also maintaining an anaerobic environment within the reactor. After the reactor was sealed, the reactor temperature was adjusted to 35℃and the rotational speed was 170rpm, and the hydraulic and anaerobic sludge retention times in the system were 8d. Taking samples every 2 days, measuring medium chain fatty acid concentration by gas chromatography, and after the reaction is stable as shown in FIG. 4, it is found that ethanol in all reactors is completely reacted, and with CO 2 The content of medium-chain fatty acid (caproic acid and caprylic acid) shows rising concentrationThe phenomenon of high post-reduction is consistent with the results under high concentration ethanol acetic acid conditions. Unlike in the case of high concentration ethanol acetic acid, more octanoic acid was found to be produced at low concentrations. The octanoic acid yield was detected in almost every group, and at 30% concentration, the octanoic acid yield reached the highest concentration, 410mg COD/L. Octanoic acid is a longer chain fatty acid than hexanoic acid, which is obtained by a further round of carbon chain extension process. The medium chain fatty acids produced by the reaction are mainly caproic acid and caprylic acid, 0%, 10%, 20%, 30%, 40%, 50%,75% group CO 2 Caproic acid (caprylic acid) produced at the content was 269 (79), 408 (193), 396 (340), 371 (407), 429 (242), 445 (197), 315 (44) mgCOD/L, respectively. The content of medium-chain fatty acid (caproic acid, caprylic acid) is CO 2 The concentration was highest at 30% and the ratio in the final product was 55.7%.
In the reactor, the electron donor is ethanol, and the electron acceptor is acetic acid. In the reaction process, ethanol can be oxidized to generate acetyl coenzyme A to participate in the subsequent carbon chain extension process to generate MCFAs (caproic acid CE), and can also be subjected to side reaction ethanol oxidation process to generate acetic acid, and the ethanol oxidation process can compete with the CE process for ethanol as an electron donor. As can be seen from FIG. 3 (a), with CO 2 Concentration increase at 30% CO 2 The highest proportion of ethanol involved in CE at the concentration. In contrast, with CO 2 The concentration increases, the ratio of oxidized ethanol to reacted ethanol decreases and then increases. That is, at 30% CO 2 At this concentration, ethanol as an electron donor is more used in the CE process than in the ethanol over-oxidation process. As can be seen from FIG. 3 (b), the amount of electron transfer in the reactor is a function of CO 2 The increase in concentration increases and then decreases. At 30% CO 2 At a concentration of 110mmol e, the highest electron transfer is present - L, compared with 0% CO 2 The increase in the condition was 18.0%. This means at 30% CO 2 Under the condition, more electrons are effectively transferred to the carbon chain extension process.
Example 3
The present embodiment provides a CO 2 Regulation of production of medium chain fatty acidsThe method comprises the following steps:
the method comprises the steps of using ethanol with the final concentration of 1000mg/L (2090 mgCOD/L) as an electron donor, using sludge fermentation organic wastewater with the final concentration of 450mg/L (482 mgCOD/L) acetic acid, 100mg/L (151 mgCOD/L) propionic acid, 100mg/L (182 mgCOD/L) butyric acid and 70mg/L (143 mgCOD/L) valeric acid as a mixed electron acceptor, adding 100g of activated sludge, wherein the Total Solid (TS) in the sludge is 0.03g/g, the Volatile Solid (VS) is 0.02g/g, the total volume of materials in the system is 2L, and further adding 2-bromoethyl sodium sulfonate, regulating the pH in a reactor to be 5.5, using different COs 2 Gas cylinders (0%, 10%, 20%, 30%, 40%, 50%, 75%) of the gas phase components purge the reactor headspace, different CO 2 The flow rate of the mixed gas is 1L/min, the purging time is 5min, and the CO in the reactor is regulated and controlled 2 The components substantially replace air while also maintaining an anaerobic environment within the reactor. After the reactor was sealed, the reactor temperature was adjusted to 35℃and the rotational speed was 170rpm, and the hydraulic power and the activated sludge retention time in the system were 15d. Samples were taken every 2 days, and the concentration of medium chain fatty acid was measured by gas chromatography, and the results are shown in fig. 5. After the waiting reaction is stable, the ethanol in all the reactors is found to be completely reacted, and along with CO 2 The increase in concentration, the increase and decrease in medium chain fatty acid (caproic acid, heptanoic acid, caprylic acid) content, which is consistent with the results obtained under carbon chain extension conditions with pure ethanol acetic acid.
Example 4
The present embodiment provides a CO 2 The method for regulating and controlling the production of the medium-chain fatty acid comprises the following steps:
ethanol with the final concentration of 1000mg/L (2090 mgCOD/L) is used as an electron donor, succinic acid with the final concentration of 300mg/L is used as an electron acceptor, 100g municipal sludge is added, the Total Solid (TS) in the sludge is 0.03g/g, the Volatile Solid (VS) is 0.02g/g, the total volume of materials in the system is 2L, nitrogen is introduced to purge the head space of the reactor, sodium bicarbonate with different contents is added, the pH value in the reactor is regulated to 5, and CO in the reactor is regulated and controlled 2 The components are 0%, 10%, 20%, 30%, 40%, 50%,75%, different COs 2 The flow rate of the mixed gas is 1L/min, the headspace time of the purging reactor is 5min, and the CO in the reactor is regulated and controlled 2 The components substantially replace air while also maintaining an anaerobic environment within the reactor. After the reactor was sealed, the reactor temperature was adjusted to 35℃and the rotational speed was 170rpm, and the hydraulic and municipal sludge retention times in the system were 15d. After the waiting reaction is stable, the ethanol in all the reactors is found to be completely reacted, and along with CO 2 The increase in concentration, the increase and decrease in the content of medium-chain fatty acids (caproic acid, caprylic acid) are observed, which is consistent with the results obtained under carbon chain extension conditions with pure ethanol acetic acid.
Example 5
The present embodiment provides a CO 2 The method for regulating and controlling the production of the medium-chain fatty acid comprises the following steps:
lactic acid with a final concentration of 4000mg/L is used as an electron donor, residual sludge is used as an electron acceptor precursor, total Solid (TS) of the residual sludge is 0.036g/g, volatile Solid (VS) is 0.020g/g, lactic acid and the residual sludge are mixed according to a volume ratio of 9:1, 200g of anaerobic sludge is added, total Solid (TS) of the anaerobic sludge is 0.027g/g, volatile Solid (VS) is 0.015g/g, total volume of materials in the system is 2L, pH in a reactor is adjusted to 5.5, sodium 2-bromoethyl sulfonate is further added, and different CO is used 2 Gas cylinders (0%, 10%, 20%, 30%, 40%, 50%, 75%) of the gas phase components purge the reactor headspace, different CO 2 The flow rate of the mixed gas is 2L/min, the purging time is 5min, and the CO in the reactor is regulated and controlled 2 The components substantially replace air while also maintaining an anaerobic environment within the reactor. After the reactor was sealed, the reactor temperature was adjusted to 35℃and the rotational speed was 170rpm, and the residence time of both the solids and anaerobic sludge in the system was 30d. After the waiting reaction was stabilized, it was found that the lactic acid was completely reacted in all the reactors, and that with CO 2 The concentration increases, and the content of medium-chain fatty acids (caproic acid, enanthic acid and caprylic acid) is increased and then decreased.
Example 6
The present embodiment provides a CO 2 The method for regulating and controlling the production of the medium-chain fatty acid comprises the following steps:
ethanol with final concentration of 1000mg/L (2090 mgCOD/L) is used as electron donor, acetic acid with final concentration of 800mg/L (856 mgCOD/L) is used as electron acceptor, and then400g of anaerobic sludge, 0.03g/g of Total Solid (TS) of the anaerobic sludge, 0.02g/g of Volatile Solid (VS) and further adding sodium 2-bromoethyl sulfonate, and carrying out long-term reaction in a 5L anaerobic sequencing batch reactor, wherein the headspace of the reactor is 1L, and the volume of a sludge-water mixture is 4L. The operation mode of the reactor is as follows: water was added for 5 minutes, the reaction was stirred for 47 hours, the mud water mixture was drained for 5 minutes, the precipitate was left to stand for 40 minutes, and drainage was performed for 10 minutes. The hydraulic retention time is controlled to be 3d, and the anaerobic sludge retention time is controlled to be 15d by controlling the water inlet and outlet of the reactor and the discharge of the sludge-water mixture. Before the reactor is started, different CO is used 2 Gas cylinders (10%, 20%, 30%, 40%, 50%, 75%) of the gas phase components purge the reactor headspace, different CO 2 The flow rate of the mixed gas is 1L/min, the purging time is 5min, and the CO in the reactor is regulated and controlled 2 The components fully replace air, and simultaneously the anaerobic environment is kept in the reactor, the pH is controlled to be 5.5, and the reactor is sealed. By means of an on-line control system during the operation of the reactor, by means of CO 2 Make-up or absorption of CO in the reactor 2 The components of (2) are controlled at initial values, the PH is controlled at 5.5 by adding acid and alkali, and the temperature is controlled at 35 ℃. When the reactor reached stability, the medium chain fatty acids (caproic acid, caprylic acid) were CO-associated 2 The content increases, and then decreases.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (5)
1. CO (carbon monoxide) 2 A method for regulating and controlling production of medium-chain fatty acid, which is characterized in that: comprising the following steps: inoculating microorganism capable of performing carbon chain extension reaction to produce medium chain fatty acid by using electron donor and electron acceptor or electron acceptor precursor as substrate, and introducing CO into reactor 2 The concentration of the water is controlled within a certain concentration range,preparing medium chain fatty acid;
the method comprises the following steps:
step 1, mixing an electron donor and an electron acceptor or an electron acceptor precursor, further adding the microorganisms, uniformly mixing, and adjusting the pH of a system to 5-6;
step 2, deoxidizing the reaction system and then removing CO 2 Reacting with nitrogen mixed gas for a period of time;
the CO 2 CO in the mixture with nitrogen 2 The gas phase content is 30%;
the electron donor is ethanol and/or lactic acid, and the electron acceptor is at least one of acetic acid, formic acid, propionic acid, butyric acid, valeric acid, succinic acid and malic acid;
the microorganism is derived from at least one of activated sludge, anaerobic sludge and municipal sludge.
2. A CO according to claim 1 2 A method for regulating and controlling production of medium-chain fatty acid, which is characterized in that: the reaction control conditions in the step 2 are as follows: aiming at an organic wastewater treatment system, the temperature is 28-40 ℃, the hydraulic retention time in the system is 2-20 d, the microorganism retention time is 5-20 d, and the stirring speed is 50-200 rpm; aiming at a solid waste treatment system, the temperature is 28-40 ℃, the solid residence time and the microorganism residence time in the system are 5-20 d, and the stirring speed is 50-200 rpm.
3. A CO according to claim 2 2 A method for regulating and controlling production of medium-chain fatty acid, which is characterized in that: and after the microorganism is added in the step 1, further adding sodium 2-bromoethyl sulfonate.
4. A CO according to claim 1 2 A method for regulating and controlling production of medium-chain fatty acid, which is characterized in that: after the microorganism is added in the step 1, 2-bromoethyl sodium sulfonate is further added, and the reaction control conditions in the step 2 are as follows: aiming at an organic wastewater treatment system, the temperature is 28-40 ℃, the hydraulic retention time in the system is 2-20 d, and the microorganism retention time is 5-40d, stirring at 50-200 rpm; aiming at a solid waste treatment system, the temperature is 28-40 ℃, and the solid residence time and the microorganism residence time are 5-40 d; the stirring speed is 50-200 rpm.
5. A CO according to claim 1 2 A method for regulating and controlling production of medium-chain fatty acid, which is characterized in that: the electron acceptor precursor is selected from at least one of municipal sludge, excess sludge, organic solid waste and organic sewage.
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| AU2023210603A AU2023210603A1 (en) | 2022-08-25 | 2023-08-02 | Method for Preparing Medium-Chain Fatty Acids (MCFAs) Based on Carbon Dioxide Regulation |
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