CN115336588A - Compound biological control agent for stone cultural relics and preparation method and application thereof - Google Patents

Compound biological control agent for stone cultural relics and preparation method and application thereof Download PDF

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Publication number
CN115336588A
CN115336588A CN202211116383.XA CN202211116383A CN115336588A CN 115336588 A CN115336588 A CN 115336588A CN 202211116383 A CN202211116383 A CN 202211116383A CN 115336588 A CN115336588 A CN 115336588A
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biological control
control agent
compound biological
rock
stone cultural
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张秉坚
朱成帅
胡瑜兰
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Zhejiang University ZJU
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
    • A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
    • A01N39/04—Aryloxy-acetic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M21/00—Apparatus for the destruction of unwanted vegetation, e.g. weeds
    • A01M21/04—Apparatus for destruction by steam, chemicals, burning, or electricity
    • A01M21/043—Apparatus for destruction by steam, chemicals, burning, or electricity by chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/48—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —S—C≡N groups
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P13/00—Herbicides; Algicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P15/00—Biocides for specific purposes not provided for in groups A01P1/00 - A01P13/00
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D133/00—Coating compositions based on homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Coating compositions based on derivatives of such polymers
    • C09D133/04—Homopolymers or copolymers of esters
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/14—Paints containing biocides, e.g. fungicides, insecticides or pesticides

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Materials Engineering (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a compound biological control agent for stone cultural relics, and a preparation method and application thereof. The compound biological control agent comprises the raw materials of, by mass, dimethyl tetrachlorodimethylamine salt, thymol, octhiolone, dithiocyano-methane, pure acrylic emulsion, ultrapure water and a cross-linking agent. The compound biological control agent has good inhibition and killing effects on fungi, bacteria, algae, moss, spores, green plants and weeds in laboratory and field tests. The compound biological control agent has stable property after being compounded, does not generate chemical change, does not change physical properties, does not generate floating oil, flocculation, precipitation or color change after being mixed, does not generate heat, generates bubbles and the like, and can be stored at normal temperature and normal pressure. Meanwhile, the application range of each component of the medicament is complementary, and the timeliness is complementary, so that most common organisms on the surface of the rock can be killed quickly, long-acting prevention and control can be formed on the surface of the rock, and secondary colonization of the organisms is prevented.

Description

Compound biological control agent for stone cultural relics and preparation method and application thereof
Technical Field
The invention belongs to the field of cultural relic protection, and particularly relates to a compound biological control agent for stone cultural relics, and a preparation method and application thereof.
Background
The immovable stone cultural relics are mostly exposed in the natural environment and directly and closely contacted with the biosphere, thereby not only providing a growing and breeding place for various organisms, but also providing nutrient substances. The growth and propagation of organisms can accelerate the weathering of rocks, and particularly hypha and rhizoid of species such as algae, fungi, lichens, moss and the like can penetrate into micropores of the rocks, so that not only can the structure be damaged, but also ions can be captured from a rock matrix to cause chemical corrosion of the rocks. Therefore, there is a need to control the growth of organisms on the surface of stone cultural relics by using safe and reliable means.
In the treatment of biological weathering of stone cultural relics, mechanical cleaning methods (manual removal, use of tools such as a dust collector, a scalpel and a shovel) are effective in a short period of time, but need frequent repeated operation, increase damage to the surface of the cultural relics and are rarely used independently. Physical methods (nano metal materials, heat shock, ultraviolet, laser radiation and the like) cannot eliminate the lithobiostructure in the fracture, have low long-term use efficiency, damage cultural relics after repeated use and are only used pertinently at present. The biological methods (competition and predation) have the advantages of less selectivity, higher implementation difficulty, narrower application range, less use and less report. Chemical methods (bacteriostatic agents, antibacterial agents, natural plant essential oils, and the like) are currently being used in the cultural heritage field, but have the following problems: the drug effect period is short, the biological control range is narrow, and the repeated use not only pollutes the environment but also changes the appearance of the cultural relic.
In order to solve the problem of cultural relic biological corrosion caused by repeated growth of biological diseases on the surface of the stone cultural relic in the open environment, the invention provides a compound biological control agent which does not change the original appearance of the cultural relic, is safe and efficient, is convenient to apply, and has lasting effect and wide application range.
Disclosure of Invention
The compound biological control agent provided by the invention does not change the original appearance of cultural relics, is safe and efficient, is convenient to apply, has a lasting effect and a wide application range, and has control effects on fungi, bacteria, algae, moss, spores, green plants and weeds. The preparation is realized by the following technical scheme:
the invention discloses a compound biological control agent for the surface of a stone cultural relic, which comprises the following raw materials in percentage by mass:
Figure BDA0003845733390000021
as a further improvement, the cross-linking agent provided by the invention is any one of dodecyl alcohol ester, benzyl alcohol BA, ethylene glycol, propylene glycol and hexanediol.
The invention also discloses a preparation method of the compound biological control agent for the surface of the stone cultural relic, which comprises the following steps:
1) Mixing dimethyltetrachlorodimethylamine salt, thymol, octhioketone and dithiocyano-methane according to a ratio to obtain a solution A;
2) Preparing the acrylic emulsion and the ultrapure water into a suspension B with the solid content of 0.5-10%;
3) Mixing the solution A and the suspension B according to a ratio to obtain a suspension C;
4) Before use, 0.1-6% of cross-linking agent is added into the suspension C to obtain the compound biological control agent D.
The invention also discloses an application method of the compound biological control agent for the surface of the stone cultural relic, which is characterized by comprising the following steps:
1) Cleaning biological debris, clay and the like adhered to the surface of the rock by using tools such as a brush and the like to ensure that the compound biological control agent is adhered to the surface;
2) And (3) performing permeation coating on the surface of the rock by using the compound biological control agent to form a biological control layer.
As a further improvement, the step of the penetration coating specifically comprises: the compound biological control agent is sucked by a rubber head dropper or a brush and is evenly coated or dripped on the surface of the rock from top to bottom until the surface of the rock is completely wetted and saturated, and the operation is repeated for 2 to 5 times, so that a rainwater erosion resistant biological control layer can be formed on the surface of the rock. The biological control effect of the invention is as follows:
the compound biological control agent has good inhibition and killing effects on fungi, bacteria, algae, moss, spores, green plants and weeds in laboratory and field tests.
The compound biological control agent has stable property after being compounded, does not generate chemical change, does not change physical properties, does not generate floating oil, flocculation, precipitation or color change after being mixed, does not generate phenomena of heating, bubble generation and the like, and can be stored at normal temperature and normal pressure. Meanwhile, the application range of each component of the medicament is complementary, and the timeliness is complementary, so that most common organisms on the surface of the rock can be killed quickly, long-acting prevention and control can be formed on the surface of the rock, and secondary colonization of the organisms is prevented.
The pure acrylic emulsion is an emulsion prepared by copolymerizing a plurality of acrylic acid, methacrylic acid, methyl methacrylate and acrylic esters, has no toxicity, no corrosion and no combustion, and belongs to a water-based non-hazardous substance. After the compound biological control agent is used, the solvent is volatilized, so that the biological control agent can be solidified, microparticles on the surface of a rock are tightly combined, a water-resistant layer can be formed on the surface of the rock, and the compound biological control agent is riveted on the surface of a cultural relic, so that a lasting biological control effect is achieved. The coating has good compatibility with cultural relics under the use concentration, is aging-resistant, water-resistant and environment-friendly.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The technical solution of the present invention is further illustrated by the following specific examples:
example 1:
1. preparation of compound biological control agent
Firstly, preparing a pure acrylic emulsion A with the solid content of 4% by using ultrapure water, then respectively adding ditetrafluoromethyl dimethylamine salt, thymol, octulone and dithiocyano-methane into the emulsion A to ensure that the mass fractions of the components in the solution are respectively 1%,1%,0.1% and 0.2%, and mixing to obtain a compound biological control agent suspension B. Adding a cross-linking agent with the volume fraction of 0.1% before use, and uniformly mixing to obtain the compound biological control agent C for protecting the stone cultural relics.
2. Biocidal efficacy test
In order to verify the killing effect of the compound biological control agent on common organisms on the surface of the cultural relic, such as bacteria, fungi, algae and moss, the following method is adopted for testing.
(1) Minimum effective concentration and bacteriostatic circle
In the laboratory, different culture media are configured to culture disease organisms taken from the surface of the cultural relic, and experiments of the minimum effective concentration and the inhibition zone of the compound biological control agent are carried out. E.g. using KNOP medium (KNO) 3 1.0g/L，Ca(NO 3 ) 2 0.1g/L，K 2 HPO 4 0.2g/L，MgSO 4 ·7H 2 O0.1g/L，FeCl 3 0.001 g/L) culture of algae and moss; preparing LB (tryptone 10g/L, yeast extract 5g/L, naCl10 g/L) culture fine powderBacteria; PDA culture medium (potato 200g/L, glucose 20g/L, agar 20g/L, peptone 5g/L, potassium dihydrogen phosphate 3g/L, magnesium sulfate 1.5 g/L) is prepared to culture fungi. The results show that the minimum inhibition concentrations of the algae, the bryophyte spores, the fungi and the bacteria are respectively 0.01 percent, 0.1 percent, 0.01 percent and 0.01 percent, and the inhibition zones of the filter paper sheets with the diameters of 20mm on the bacteria and the fungi are respectively 46.63 +/-0.4 mm and 55.65 +/-0.31 mm.
(2) In situ experiment
The compound biological control agent is applied to places with algae, lichen, moss and fern diseases on the surface of the carbonate stone cultural relic in a coating and spraying mode. Scraping the algae on the rock surface by adopting a cotton swab smearing mode, and extracting chlorophyll fluorescence of the algae on the rock surface by using a DMSO extraction method. The comparison of seven days shows that the chlorophyll fluorescence content on the surface of the rock is from 2.76ug/cm 2 And reduced to 0. The lichen biological community on the rock surface is browned and falls off, the moss and fern begin to turn yellow 7 days later, and completely withers and dies 30 days later and gradually falls off.
(3) Water resistance test
The compound biological control agent is smeared on a carbonate rock sample block, the color difference change before and after smearing is delta E =1.22, after 24 soaking-drying cycles, a biological control film formed on the surface completely disappears, and each cycle comprises 12h of soaking and 12h of drying (65 ℃).
The compound biological control agent is used on the carbonate rock in the field, and the organisms such as algae, moss, lichen and the like are not recovered after half a year of monitoring, so that a good inhibition effect is obtained.
Example 2:
1. preparation of compound biological control agent
Firstly, preparing a pure acrylic emulsion A with the solid content of 6% by using ultrapure water, then respectively adding dimethyl tetrachlorodimethylamine salt, thymol, octulone and dithiocyano-methane into the emulsion A to ensure that the mass fractions of the components are respectively 2%,2%,0.2% and 0.4%, and mixing to obtain a suspension B. Adding a cross-linking agent with the volume fraction of 0.1% before use, and uniformly mixing to obtain the compound biological control agent for protecting the stone cultural relics.
2. Biocidal efficacy test
Different culture mediums are configured in a laboratory, various disease organisms separated from the surface of the cultural relic are cultured, and the compound biological control agent is added into the culture mediums to determine the minimum effective concentration and the inhibition zone of the compound biological control agent. The experimental result shows that the inhibition zones of the filter paper sheet with the diameter of 20mm for bacteria and fungi are 49.43 +/-0.21 mm and 56.99 +/-0.11 mm respectively.
The compound biological control agent is applied to places with algae, lichen, moss and fern diseases on the surface of carbonate stone cultural relics in a coating and spraying mode. Scraping algae with cotton swab, extracting chlorophyll fluorescence with DMSO extraction method, wherein the chlorophyll fluorescence content of algae is 2.16ug/cm after seven days 2 Is reduced to 0; browning and shedding of lichen colonies; the moss and the fern begin to turn yellow 7 days later, and completely wither and die 30 days later and gradually fall off.
The compound biological control agent is smeared on a carbonate stone sample block, the color difference before and after smearing is changed to delta E =3.05, after 28 dry-wet cycle experiments, the biological control layer completely disappears, and each cycle comprises 12h of soaking and 12h of drying (65 ℃). The compound biological control agent is used on the surface of the field carbonate stone cultural relic, and the algae, the moss and the lichen are not recovered after half-year monitoring, so that the biology is well inhibited.
Control case 1:
the same procedure as in example 1 was followed except that the solid content of the pure acrylic emulsion in emulsion A was increased to 10%. As a result, the compound biological control agent can form dazzling phenomenon and change appearance on the surface of the carbonate stone cultural relic. This phenomenon is particularly severe on uneven rock surfaces, and the applied agent forms a thick film on the rock surface due to the accumulated liquid in the gullies, which causes the glare phenomenon. Indicating that the pure acrylic emulsion with higher concentration can cause the change of the appearance of the cultural relics.
Control case 2:
in the same way as embodiment 1, the ultrapure water is used to replace the emulsion A to prepare the compound biological control agent without adding the pure acrylic emulsion. The results show that after 45 days, due to rain wash, the components of the compound biological control agent cannot be detected on the rock surface, algae begin to be re-planted on the rock surface after 3 months, and the algae are completely re-planted after 6 months. The pure acrylic emulsion has an important function in prolonging the bacteriostatic effect of the compound biological control agent.
Finally, it should be noted that the above-mentioned list is only a specific embodiment of the present invention. It is obvious that the invention is not limited to the above embodiments, but that many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.

Claims (5)

1. A compound biological control agent for the surface of stone cultural relics is characterized by comprising the following raw materials in percentage by mass:
Figure FDA0003845733380000011
2. the compound biological control agent for the surface of the stone cultural relic as claimed in claim 1, wherein the cross-linking agent is any one of dodecyl alcohol ester, benzyl alcohol BA, ethylene glycol, propylene glycol and hexylene glycol.
3. A method for preparing the compound biological control agent for the surface of the stone cultural relic as claimed in claim 1 or 2, which comprises the following steps:
1) Mixing dimethyltetrachlorodimethylamine salt, thymol, octhioketone and dithiocyano-methane according to a ratio to obtain a solution A;
2) Preparing a suspension B with the solid content of 0.5% -10% from the acrylic emulsion and the ultrapure water;
3) Mixing the solution A and the suspension B according to a ratio to obtain a suspension C;
4) Before use, the cross-linking agent (0.1-6%) is added into the suspension C to obtain the compound biological control agent D.
4. The application method of the compound biological control agent for the surface of the stone cultural relic as claimed in claim 1 or 2, which comprises the following steps:
1) Cleaning biological debris, clay and the like adhered to the surface of the rock by using tools such as a brush and the like to ensure that the compound biological control agent is adhered to the surface;
2) And (3) performing permeable coating on the surface of the rock by using the compound biological control agent to form a biological control layer.
5. The application method of the compound biological control agent for the surface of the stone cultural relic as claimed in claim 4, wherein the penetration coating step is specifically as follows: the compound biological control agent is absorbed by a rubber head dropper or a hairbrush and is evenly coated or dripped on the surface of the rock from top to bottom until the surface of the rock is completely wetted and saturated, and the operation is repeated for 2 to 5 times, so that a rainwater erosion resistant biological control layer can be formed on the surface of the rock.
CN202211116383.XA 2022-06-12 2022-09-14 Compound biological control agent for stone cultural relics and preparation method and application thereof Pending CN115336588A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773144A (en) * 2013-10-30 2014-05-07 北京工业大学 Waterborne protectant for stone cultural objects

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773144A (en) * 2013-10-30 2014-05-07 北京工业大学 Waterborne protectant for stone cultural objects

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
THE DOW CHEMICAL COMPANY: "PRIMAL™ E-2310H Emulsion", pages 1 - 2, Retrieved from the Internet <URL:https://www.dow.com/zh-cn/search.html#q=E-2310h&t=All&sort=relevancy> *
张海英;李强;张秉坚;胡瑜兰;: "露天石质文物表面苔藓的科学认知和防治技术研究进展", no. 08, pages 18 - 23 *
栗荣贺;: "简述文物保护用丙烯酸树脂Paraloid B-72", vol. 1, no. 00, pages 237 - 243 *
武望婷;何秋菊;闫丽;: "浅析文物保护中的防霉及杀菌防霉剂的应用", no. 00, pages 439 - 444 *

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