CN115322905A - Formaldehyde degradation strain breeding method, immobilized microorganism filler and air purifier - Google Patents
Formaldehyde degradation strain breeding method, immobilized microorganism filler and air purifier Download PDFInfo
- Publication number
- CN115322905A CN115322905A CN202210923320.9A CN202210923320A CN115322905A CN 115322905 A CN115322905 A CN 115322905A CN 202210923320 A CN202210923320 A CN 202210923320A CN 115322905 A CN115322905 A CN 115322905A
- Authority
- CN
- China
- Prior art keywords
- formaldehyde
- strain
- temperature
- strains
- flora
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F8/00—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying
- F24F8/10—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by separation, e.g. by filtering
- F24F8/175—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by separation, e.g. by filtering using biological materials, plants or microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention discloses a formaldehyde degrading strain breeding method, immobilized microbial filler and an air purifier. According to the invention, through multilayer selection, a formaldehyde degradation target strain with good formaldehyde resistance, strong degradation capability and low lowest active temperature gradient can be selected, and because the activity of the strain can be increased along with the increase of the temperature within a certain temperature range, but the decrease of the indoor temperature can greatly influence the activity of the strain, the strain with the lowest active temperature as low as possible is selected as the target strain, so that the strain can keep high activity and high degradation capability at the indoor temperature of a large part of areas.
Description
Technical Field
The invention relates to a microbial treatment technology for environmental pollution, in particular to a formaldehyde degrading strain breeding method, an immobilized microbial filler and an air purifier.
Background
Although the decoration material contains formaldehyde, volatile organic compounds and the like, the ventilation treatment mode is generally adopted, but the overflow time period of the formaldehyde and the volatile organic compounds is long, and the formaldehyde and the volatile organic compounds can not be treated in a short time.
Disclosure of Invention
One of the technical problems to be solved by the present invention is to provide a method for breeding formaldehyde degrading strains, so as to solve the technical problems in the background art.
The technical scheme of the invention is as follows:
a formaldehyde degradation strain breeding method comprises the following steps:
(1) Collecting formaldehyde degrading flora, performing classified propagation cultivation, respectively inoculating the flora to a selective culture medium containing 55-60mg/L of formaldehyde for cultivation when the flora is in a logarithmic growth period, and determining the formaldehyde content and the flora density of the selective culture medium after the flora is completely propagated and stabilized;
(2) Selecting a plurality of strains with high flora density and low formaldehyde in a selective culture medium as preselected strains;
(3) Respectively adding formaldehyde solution into the selective culture medium of the preselected strains to the concentration of 55-60mg/L, dividing the culture medium into a plurality of temperature points at 9-30 ℃ for constant temperature culture, recording the content of formaldehyde after each culture is finished, and re-adding the formaldehyde solution to the concentration of 55-60mg/L;
(4) Selecting the lowest active temperature gradient of each group of strains, comparing the formaldehyde concentration of the selective culture medium, sequencing from low to high in sequence, selecting the first strain as a target strain when the first optimal degradation temperature gradient is less than or equal to the second optimal degradation temperature gradient, selecting the second strain as the target strain if the first temperature is higher than the second temperature by one gradient and the difference value of the formaldehyde concentration is smaller, and comparing and selecting the strains in the same method in subsequent ranking.
Furthermore, not less than five strains with lower formaldehyde content are selected in the step 2.
Further, in step 3, 9-30 ℃ is divided into temperature gradients every 3 ℃.
Further, in step 4, if the difference of the formaldehyde concentrations is within 5%, the difference is considered to be small.
The second technical problem solved by the invention is to provide an immobilized microorganism filler.
The technical scheme of the invention is as follows:
an immobilized microorganism filler, wherein a target strain selected by the formaldehyde degradation strain breeding method is used as an adsorption flora.
The invention solves the technical problem of providing an air purifier.
The technical scheme of the invention is as follows:
an air purifier comprises a biological processor made of the immobilized microorganism filler.
Furthermore, a constant temperature heater is arranged in front of an air inlet of the biological processor, and the heating temperature is the lowest active temperature of the target strain.
The invention has the advantages that:
according to the invention, through multilayer selection, a formaldehyde degradation target strain with good formaldehyde resistance, strong degradation capability and low lowest active temperature gradient can be selected, and because the activity of the strain can be increased along with the increase of the temperature within a certain temperature range, but the decrease of the indoor temperature can greatly influence the activity of the strain, the strain with the lowest active temperature as low as possible is selected as the target strain, so that the strain can keep high activity and high degradation capability at the indoor temperature of a large part of areas.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, and is not intended to limit the present invention. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
A formaldehyde degradation strain breeding method comprises the following steps:
(1) Collecting formaldehyde degrading flora, performing classified propagation cultivation, wherein the degrading flora can be collected in activated sludge of sewage treatment plants, garbage filling plants and the like or obtained by other existing ways, when the flora is in logarithmic growth period, the degrading flora is respectively inoculated into a selective culture medium containing 55-60mg/L of formaldehyde for cultivation, and after the flora is completely propagated and stabilized, the formaldehyde content and the flora density of the selective culture medium are measured;
(2) Selecting a plurality of strains with high flora density and low formaldehyde in a selective culture medium as preselected strains, wherein the selected strains are not less than five strains for the purpose of having enough comparison flora in the subsequent selection process, and the process firstly needs to meet the requirement of high flora density because the flora can actively reproduce and live in the formaldehyde environment, has good formaldehyde resistance and secondly has the degradation capability of the formaldehyde;
(3) Respectively adding formaldehyde solution into a selective culture medium of a preselected strain to the concentration of 55-60mg/L, dividing the culture medium into a plurality of temperature points at 9-30 ℃ for constant temperature culture, recording the formaldehyde content after each culture because the comparison is not obvious when the temperature difference is small and dividing the culture medium into a temperature gradient and seven temperature gradients at intervals of 3 ℃, and adding the formaldehyde solution to the concentration of 55-60mg/L again, wherein the temperature can be from low to high, the culture time is 24-48 hours, and the culture time of all strains is ensured to be consistent;
(4) Selecting the lowest active temperature gradient of each group of strains (when the cultivation temperature of the same strains reaches a certain value, the degradation capability of the same strains is quickly improved to reach a first peak value, the degradation capability is continuously improved along with the temperature improvement, but the effect is not obvious, the temperature of the first peak value is the lowest active temperature gradient), comparing the formaldehyde concentrations of the selective culture media, and sequencing from low to high in sequence, when the first optimal degradation temperature gradient is smaller than or equal to the second optimal degradation temperature gradient, selecting the first strain as a target strain, if the first temperature is higher than the second temperature by a gradient and the formaldehyde concentration difference is smaller (the formaldehyde concentration difference is within 5%, the difference is considered smaller, the difference can be adjusted according to specific conditions), selecting the second strain as the target strain, and comparing and selecting the same strains by adopting the same method in sequence in subsequent rows.
For ease of understanding, a set of data is now provided by reference:
| index (es) | Strain A | Strain B | Strain C | Strain D | Strain E |
| Minimum active temperature gradient | 21 | 18 | 15 | 18 | 24 |
| Formaldehyde concentration (mg/L) | 8.2 | 8.5 | 9.2 | 10.3 | 11.4 |
The lowest formaldehyde concentration of the strain A is the best, but the lowest active temperature gradient of the strain B is smaller than that of the strain A, and the formaldehyde concentration difference is = (8.5-8.2)/8.2 x 100% =3.66%
Although the formaldehyde degrading ability of the strain B is poor, the formaldehyde degrading ability of the strain B is not very different from that of the strain A (the predetermined 5 percent standard is not met), but the strain B has a smaller minimum active temperature gradient, and the strain B is preferably selected.
The lowest active temperature gradient of the strain C is smaller than that of the strain B,
difference in formaldehyde concentration = (9.2-8.5)/8.5 × 100% =8.2%
Although strain C has a smaller minimum active temperature gradient than strain B, the difference between the formaldehyde degradation capacity of strain C and that of strain A is not large (exceeding the predetermined 5% criterion), and strain B is preferably selected.
In conclusion, the strain B is selected as the target strain, and the aim of the method is to select the strain with relatively low minimum active temperature gradient on the basis of relatively high formaldehyde degradation capability.
Because the activity of the flora increases along with the increase of the temperature within a certain temperature range (the indoor temperature is the highest and generally not more than the temperature range, and the upper limit value can be about 50 ℃), but the reduction of the indoor temperature can greatly influence the activity of the flora, a strain with the lowest activity temperature as low as possible is selected as a target strain, so that the strain can keep high activity and high degradation capability at the indoor temperature of a large part of regions.
The second technical problem solved by the invention is to provide an immobilized microorganism filler.
The technical scheme of the invention is as follows:
an immobilized microorganism filler, wherein a target strain selected by the formaldehyde degradation strain breeding method is used as an adsorption flora.
The invention solves the technical problem of providing an air purifier.
The technical scheme of the invention is as follows:
an air purifier comprises a biological processor made of the immobilized microorganism filler, and as a further optimization scheme, a constant temperature heater is arranged in front of an air inlet of the biological processor, the heating temperature is the lowest active temperature of a target strain, the constant temperature heater is used for heating air to the lowest active temperature of flora when the indoor temperature is particularly low, and as the flora with the lowest active temperature is selected, the flora with the lower lowest active temperature does not need to be started under the ordinary room temperature condition if no active temperature rise requirement exists.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, and these embodiments are still within the scope of the invention.
Claims (7)
1. A formaldehyde degradation strain breeding method is characterized by comprising the following steps:
(1) Collecting formaldehyde degrading flora, performing classified propagation cultivation, respectively inoculating the flora to a selective culture medium containing 55-60mg/L of formaldehyde for cultivation when the flora is in a logarithmic growth period, and determining the formaldehyde content and the flora density of the selective culture medium after the flora is completely propagated and stabilized;
(2) Selecting a plurality of strains with high flora density and low formaldehyde in a selective culture medium as preselected strains;
(3) Respectively adding formaldehyde solution into the selective culture medium of the preselected strains to the concentration of 55-60mg/L, dividing the culture medium into a plurality of temperature points at 9-30 ℃ for constant temperature culture, recording the content of formaldehyde after each culture is finished, and re-adding the formaldehyde solution to the concentration of 55-60mg/L;
(4) Selecting the lowest active temperature gradient of each group of strains, comparing the formaldehyde concentration of the selective culture medium, sequencing from low to high in sequence, selecting the first strain as a target strain when the first optimal degradation temperature gradient is less than or equal to the second optimal degradation temperature gradient, selecting the second strain as the target strain if the first temperature is higher than the second temperature by one gradient and the difference value of the formaldehyde concentration is smaller, and comparing and selecting the strains in the same method in subsequent ranking.
2. The strain breeding method for the microbial air purification filler according to claim 1, wherein the strain breeding method comprises the following steps: and (3) selecting not less than five strains with lower formaldehyde content in the step (2).
3. The method for breeding strains for microbial air purification fillers according to claim 1, wherein the method comprises the following steps: in step 3, dividing the temperature of 9-30 ℃ into a temperature gradient every 3 ℃.
4. The method for breeding strains for microbial air purification fillers according to claim 1, wherein the method comprises the following steps: in the step 4, if the difference of the formaldehyde concentration is within 5%, the difference is considered to be small.
5. An immobilized microorganism filler, which is characterized in that: the formaldehyde degrading bacteria breeding method of any one of claims 1 to 4 is adopted to select a target bacteria as an adsorption bacteria group.
6. An air purifier, its characterized in that: a biological processor made of the immobilized microorganism filler of claim 5.
7. The air purifier of claim 6, wherein: a constant temperature heater is arranged in front of an air inlet of the biological processor, and the heating temperature is the lowest active temperature of the target strains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210923320.9A CN115322905A (en) | 2022-08-02 | 2022-08-02 | Formaldehyde degradation strain breeding method, immobilized microorganism filler and air purifier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210923320.9A CN115322905A (en) | 2022-08-02 | 2022-08-02 | Formaldehyde degradation strain breeding method, immobilized microorganism filler and air purifier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115322905A true CN115322905A (en) | 2022-11-11 |
Family
ID=83920653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210923320.9A Pending CN115322905A (en) | 2022-08-02 | 2022-08-02 | Formaldehyde degradation strain breeding method, immobilized microorganism filler and air purifier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115322905A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515352A (en) * | 2003-08-28 | 2004-07-28 | 上海交通大学 | Preparation method of load type photocatalytic purification net block |
| CN113041789A (en) * | 2021-03-11 | 2021-06-29 | 黑龙江工业学院 | Formaldehyde removing device |
-
2022
- 2022-08-02 CN CN202210923320.9A patent/CN115322905A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515352A (en) * | 2003-08-28 | 2004-07-28 | 上海交通大学 | Preparation method of load type photocatalytic purification net block |
| CN113041789A (en) * | 2021-03-11 | 2021-06-29 | 黑龙江工业学院 | Formaldehyde removing device |
Non-Patent Citations (5)
| Title |
|---|
| 付秋玥: "低温纤维素降解菌的筛选及其混合发酵效果研究", 中国优秀硕士学位论文全文数据库（电子期刊）工程科技Ⅰ辑, no. 10, pages 14 * |
| 张明;王洁;孙石;申艳萍;张艮林;邹平;毕晓伊;: "甲醛降解功能菌的分离鉴定及降解特性", 环境保护科学, no. 02, pages 65 - 70 * |
| 雷得定;张志国;刘波;杨兴昊;朱南峰;: "高效甲醛降解菌的分离筛选及应用", 中国人造板, vol. 25, no. 09, pages 1 * |
| 黄赛花;陈能场;胡文锋;周建民;毕洪亮;: "一株甲醛降解真菌Aspergillus spp.H4的分离鉴定", 生态环境, no. 04, pages 1175 - 1179 * |
| 齐枝花;张晓虎;张胜华;: "微生物反应器处理空气甲醛的影响因素分析", 环境科学与技术, vol. 38, no. 05, pages 110 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Microbial inoculation influences bacterial community succession and physicochemical characteristics during pig manure composting with corn straw | |
| Wang et al. | Microbial abundance and community in subsurface flow constructed wetland microcosms: role of plant presence | |
| Marzorati et al. | How to get more out of molecular fingerprints: practical tools for microbial ecology | |
| Burke et al. | Interactions among plant species and microorganisms in salt marsh sediments | |
| Carelli et al. | Genetic diversity and dynamics of Sinorhizobium meliloti populations nodulating different alfalfa cultivars in Italian soils | |
| Do et al. | Role of Rhodobacter sp. strain PS9, a purple non-sulfur photosynthetic bacterium isolated from an anaerobic swine waste lagoon, in odor remediation | |
| CN106676022A (en) | Method for preparing microbe bacteria liquid for treating black-odor riverway | |
| Zhao et al. | Genome-centered omics insight into the competition and niche differentiation of Ca. Jettenia and Ca. Brocadia affiliated to anammox bacteria | |
| Lv et al. | Metagenomic analysis of the sludge microbial community in a lab-scale denitrifying phosphorus removal reactor | |
| Sun et al. | Effects of influent immigration and environmental factors on bacterial assembly of activated sludge microbial communities | |
| Cai et al. | Effect of nitrogen application rate on soil fungi community structure in a rice-fish mutualistic system | |
| He et al. | Community diversity and biofilm characteristic response to low temperature and low C/N ratio in a suspended carrier biofilm reactor | |
| CN115322905A (en) | Formaldehyde degradation strain breeding method, immobilized microorganism filler and air purifier | |
| CN107858305A (en) | One plant of tetracycline efficient degradation bacterium and its application | |
| Khan et al. | Assessing methanogenic archaeal community in full scale anaerobic sludge digester systems in Dubai, United Arab Emirates | |
| Nomoto et al. | Defining microbial community composition and seasonal variation in a sewage treatment plant in India using a down-flow hanging sponge reactor | |
| CN112226430A (en) | Immobilized microbial preparation and method for repairing petroleum-polluted soil by using same | |
| CN110156276A (en) | A kind of fecaluria mixed processing technique | |
| CN112358029A (en) | Novel biological composite filler and preparation method and application thereof | |
| Pires et al. | Assessing the efficiency in assisted depuration of coffee processing wastewater from mixed wild microbial selected inoculum | |
| Wolcott et al. | Suitability of select media for use in a novel green wall system used to treat brewery wastewater | |
| Luo et al. | Recent advances in research on microbial community in the composting process | |
| CN115491312A (en) | Preparation method and application of aerobic denitrifying bacteria-chlorella algae biomembrane | |
| CN110129233B (en) | Microbial agent for preparing fermentation bed padding for cage-raised chickens and preparation method thereof | |
| Liu et al. | Environmental drivers and interaction mechanisms of heavy metal and antibiotic resistome exposed to amoxicillin during aerobic composting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |