CN115317433A - Preparation process of stem cell preparation for beautifying and resisting aging - Google Patents
Preparation process of stem cell preparation for beautifying and resisting aging Download PDFInfo
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- CN115317433A CN115317433A CN202210864755.0A CN202210864755A CN115317433A CN 115317433 A CN115317433 A CN 115317433A CN 202210864755 A CN202210864755 A CN 202210864755A CN 115317433 A CN115317433 A CN 115317433A
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- stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Abstract
The invention relates to a stem cell preparation for beautifying and resisting aging, belonging to the technical field of skin care products, which comprises the following raw materials in parts by weight: 83-94% of stem cells, 0.1-1% of sodium hyaluronate, 2-6% of cell growth factors, 1-3% of hyaluronic acid and 2.9-7% of placenta polypeptides, wherein 83-94% of the stem cells comprise mesenchymal stem cells from umbilical cord, adipose mesenchymal stem cells, placenta and the like, 83-94% of the stem cells are cells with unlimited or immortal self-renewal capacity and can generate at least one type of highly differentiated progeny cells, and 2.9-7% of the placenta polypeptides are small molecular active functional polypeptides with the molecular weight of only 3000 daltons extracted from 8000 various nutritional ingredients contained in the placenta. The preparation process of the stem cell preparation for beautifying and resisting aging has good function of resisting aging, and accelerates the preparation efficiency.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to a preparation process of a stem cell preparation for beautifying and resisting aging.
Background
The skin care product, namely the product for protecting the skin, is not a product which is only used by rich people in the past any more along with the continuous progress of social economy and the abundance of material life, and the skin care product has come into common people nowadays, thereby playing a great role in improving the spirit and the image of people.
In recent years, a large number of skin care essences emerge from the beauty market, but the active ingredients of the existing essences are plant ingredient extracts, in order to improve the beauty effect of cosmetics, most of the existing essences contain lead, mercury, alcohol, preservatives, hormones, spices and other substances harmful to skin, so that when a consumer uses the essences to maintain the skin, the skin is greatly damaged, even color spots, red blood streaks, allergy and other problems are caused, the health of the consumer is greatly threatened, and a preparation process of a beauty and anti-aging stem cell preparation is provided to solve the problems.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a preparation process of a stem cell preparation for beautifying and resisting aging, which has the advantages of good anti-aging performance and the like and solves the problem of poor anti-aging performance.
(II) technical scheme
In order to realize the purpose of good anti-aging performance of the stem cell preparation, the invention provides the following technical scheme: a stem cell preparation for beautifying and resisting aging comprises the following raw materials in parts by weight: 83 to 94 percent of stem cells, 0.1 to 1 percent of sodium hyaluronate, 2 to 6 percent of cell growth factors, 1 to 3 percent of hyaluronic acid and 2.9 to 7 percent of placenta polypeptide.
Preferably, the stem cells 83-94% include, but are not limited to umbilical cord mesenchymal stem cells, adipose mesenchymal stem cells, placenta and other derived mesenchymal stem cells.
Preferably, the stem cells 83-94% are a class of cells with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells.
Preferably, 2.9-7% of the placenta polypeptide is small molecule active functional polypeptide with molecular weight of only 3000 daltons extracted from more than 8000 nutrient components contained in the placenta.
Preferably, the feed comprises the following raw materials in parts by weight: stem cells 83, sodium hyaluronate 1%, cell growth factors 6%, hyaluronic acid 3% and placenta polypeptides 7%.
The invention aims to solve another technical problem of providing a preparation process of a stem cell preparation for beautifying and resisting aging, which comprises the following steps:
1) Preparing raw materials: preparing stem cells, sodium hyaluronate, cell growth factors, hyaluronic acid and placenta polypeptide, and then separating the stem cells;
2) Culturing: subculturing and culturing stem cells to 5-8 generations, collecting the obtained stem cells, culturing with a culture medium, cleaning with sterile PBS, centrifuging, precipitating, shaking in a low-sugar DMEM culture medium containing FBS and green chain double antibody, inoculating the stem cells into a culture dish rich in amino acid for culturing, digesting with pancreatin after the stem cells are fused to 80%, subculturing to obtain a stem cell culture solution, and collecting a mixture of the stem cells and a supernatant in the stem cell culture solution.
3) Ultrasonic cracking: and ultrasonically cracking the mixture of the stem cells and the supernatant, centrifuging to obtain the supernatant, and taking out cell debris.
4) And (3) filtering and concentrating: filtering with sterile filter membrane, concentrating in an ultrafiltration centrifugal tube, transferring the concentrated solution into an ultracentrifuge tube added with sucrose with the mass concentration of 300g/L, centrifuging, diluting the bottom precipitate with PBS, concentrating again with filter membrane, filtering and sterilizing to obtain the essence stem cell preparation.
5) Mixing: mixing the prepared essence stem cell preparation, sodium hyaluronate, cell growth factor, hyaluronic acid and placenta polypeptide, and stirring to obtain the final product.
(III) advantageous effects
Compared with the prior art, the invention provides a preparation process of a stem cell preparation for beautifying and resisting aging, which has the following beneficial effects:
1. according to the preparation process of the stem cell preparation for beautifying and resisting aging, only stem cells, sodium hyaluronate, cell growth factors, hyaluronic acid and placenta polypeptide are used during preparation, and substances harmful to skin, such as lead, mercury, alcohol, preservatives, hormones, spices and the like are not added in the stem cell preparation, so that the stem cell preparation has good anti-aging performance.
2. According to the preparation process of the beauty anti-aging stem cell preparation, when the stem cell preparation is prepared, only the stem cells need to be processed, the stem cells are purified firstly, then are cultured, and finally are subjected to ultrasonic cracking and filtering concentration to obtain an essence stem cell preparation, and then a finished product is obtained by mixing and stirring the essence stem cell preparation with sodium hyaluronate, cell growth factors, hyaluronic acid and placenta polypeptide, so that the preparation efficiency is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: a stem cell preparation for beautifying and resisting aging comprises the following raw materials in parts by weight: 83 to 94 percent of stem cells, 0.1 to 1 percent of sodium hyaluronate, 2 to 6 percent of cell growth factors, 1 to 3 percent of hyaluronic acid and 2.9 to 7 percent of placenta polypeptide.
Wherein, the stem cells: the stem of a stem cell, translated by the english term stem, means the stem, stem and origin, and the function of the stem cell population is to control and maintain the regeneration of the cell, and in general, between the stem cell and its terminally differentiated progeny there is an intermediate progenitor cell population called "committed progenitor cell" which has limited expansion capacity and limited differentiation potential, the function of these cell populations is to increase the number of differentiated cells produced after each division of the stem cell, which has the capacity to self-renew, but the division of the stem cell is in fact relatively asymmetric.
Wherein, the ratio of sodium hyaluronate: sodium hyaluronate, with the chemical formula of (C14H 20NO11 Na) n, is an inherent component in human body, is a kind of glucuronate, has NO species specificity, is widely present in tissues and organs such as placenta, amniotic fluid, crystalline lens, articular cartilage, skin dermis and the like, is distributed in cytoplasm and intercellular substance, and plays a role in lubricating and nourishing cells and organs contained in the sodium hyaluronate.
Wherein, the cell growth factor: the cell growth factor generally refers to interleukin-2, interleukin-2 is a kind of cytokine of chemotactic factor family, it is by the source of multicellular (mainly produced by activated T cell), the cell factor with pleiotropic effects (mainly promote lymphocyte growth, proliferate, differentiate), have important effects on immune response and antiviral infection of the organism, can stimulate the T cell already promoted by specific antigen or mitogenic factor to proliferate; can activate T cells and promote cytokine production; stimulating NK cell proliferation, enhancing NK killing activity, generating cytokines, and inducing LAK cell generation; promoting B cell proliferation and antibody secretion; macrophages are activated.
Wherein, hyaluronic acid: hyaluronic acid is also called hyaluronic acid, which is an acidic mucopolysaccharide, meyer et al, university of Columbia, USA, in 1934, first separates the substance from the vitreous humor of the cow's eye, and hyaluronic acid shows various important physiological functions in the organism by its unique molecular structure and physicochemical properties, such as joint lubrication, vascular wall permeability regulation, protein regulation, water electrolyte diffusion and movement regulation, and wound healing promotion.
Wherein, the placenta polypeptide: the placenta polypeptide is small molecule active functional polypeptide with molecular weight of 3000 dalton extracted from 8000 kinds of nutrient components in placenta, and can be used for intramuscular injection and direct intravenous injection. The drug effect stability is good, the targeting is strong, no obvious side effect is seen in clinic, and the drug can be applied to clinic treatment and has the functions of health care and prevention.
The invention aims to solve another technical problem of providing a preparation process of a stem cell preparation for beautifying and resisting aging, which comprises the following steps:
1) Preparing raw materials: preparing stem cells, sodium hyaluronate, cell growth factors, hyaluronic acid and placenta polypeptide, and then separating the stem cells;
2) Culturing: subculturing and culturing stem cells to 5-8 generations, collecting the obtained stem cells, culturing the stem cells by using a culture medium, cleaning the stem cells by using sterile PBS, centrifuging and precipitating, uniformly shaking the stem cells in a low-sugar DMEM culture medium containing FBS and green chain double antibody, inoculating the stem cells into a culture dish rich in amino acid for culturing, digesting the stem cells by using pancreatin after the stem cells are fused to 80%, subculturing to obtain a stem cell culture solution, and collecting a mixture of the stem cells in the stem cell culture solution and a supernatant.
3) Ultrasonic cracking: the mixture of stem cells and supernatant was sonicated, and the supernatant was obtained by centrifugation, and cell debris was taken out.
4) And (3) filtering and concentrating: filtering with sterile filter membrane, concentrating in an ultrafiltration centrifugal tube, transferring the concentrated solution into an ultracentrifuge tube added with sucrose with the mass concentration of 300g/L, centrifuging, diluting the bottom precipitate with PBS, concentrating again with filter membrane, filtering and sterilizing to obtain the essence stem cell preparation.
5) Mixing: mixing the prepared essence stem cell preparation, sodium hyaluronate, cell growth factor, hyaluronic acid and placenta polypeptide, and stirring to obtain the final product.
Example two: a stem cell preparation for beautifying and resisting aging comprises the following raw materials in parts by weight: stem cells 83, sodium hyaluronate 1%, cell growth factors 6%, hyaluronic acid 3% and placenta polypeptides 7%.
The other process flows of the first embodiment are the same as those of the first embodiment, and are not described in detail herein.
Example three: 94% of stem cells, 0.1% of sodium hyaluronate, 2% of cell growth factors, 1% of hyaluronic acid and 2.9% of placenta polypeptides.
The other process flows of the first embodiment are the same as those of the first embodiment, and are not described in detail herein.
The invention has the beneficial effects that:
1. according to the preparation process of the stem cell preparation for beautifying and resisting aging, only stem cells, sodium hyaluronate, cell growth factors, hyaluronic acid and placenta polypeptide are used during preparation, and substances harmful to skin, such as lead, mercury, alcohol, preservatives, hormones, spices and the like are not added in the stem cell preparation, so that the stem cell preparation has good anti-aging performance.
2. According to the preparation process of the beauty anti-aging stem cell preparation, when the stem cell preparation is prepared, only the stem cells need to be processed, the stem cells are purified firstly, then are cultured, and finally are subjected to ultrasonic cracking and filtering concentration to obtain an essence stem cell preparation, and then a finished product is obtained by mixing and stirring the essence stem cell preparation with sodium hyaluronate, cell growth factors, hyaluronic acid and placenta polypeptide, so that the preparation efficiency is improved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The stem cell preparation for beautifying and resisting aging is characterized by comprising the following raw materials in parts by weight: 83 to 94 percent of stem cells, 0.1 to 1 percent of sodium hyaluronate, 2 to 6 percent of cell growth factors, 1 to 3 percent of hyaluronic acid and 2.9 to 7 percent of placenta polypeptide.
2. The preparation of stem cells for beauty treatment and anti-aging according to claim 1, wherein the stem cells comprise 83-94% of mesenchymal stem cells from umbilical cord, adipose mesenchymal stem cells, placenta, etc.
3. The cosmetic anti-aging stem cell preparation according to claim 1, wherein 83-94% of the stem cells are a type of cells with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells.
4. The preparation of stem cells for beauty treatment and anti-aging according to claim 1, wherein 2.9-7% of the placenta polypeptides are small active functional polypeptides with molecular weight of only 3000 daltons extracted from more than 8000 kinds of nutrients contained in the placenta.
5. The stem cell preparation for beauty treatment and anti-aging according to claim 1, which is characterized by comprising the following raw materials in parts by weight: stem cells 83, sodium hyaluronate 1%, cell growth factors 6%, hyaluronic acid 3% and placenta polypeptides 7%.
6. A preparation process of a stem cell preparation for beautifying and resisting aging is characterized by comprising the following steps:
1) Preparing raw materials: preparing stem cells, sodium hyaluronate, cell growth factors, hyaluronic acid and placenta polypeptide, and then separating the stem cells;
2) Culturing: subculturing and culturing stem cells to 5-8 generations, collecting the obtained stem cells, culturing with a culture medium, cleaning with sterile PBS, centrifuging, precipitating, shaking in a low-sugar DMEM culture medium containing FBS and green chain double antibody, inoculating the stem cells into a culture dish rich in amino acid for culturing, digesting with pancreatin after the stem cells are fused to 80%, subculturing to obtain a stem cell culture solution, and collecting a mixture of the stem cells and a supernatant in the stem cell culture solution.
3) Ultrasonic cracking: and ultrasonically cracking the mixture of the stem cells and the supernatant, centrifuging to obtain the supernatant, and taking out cell debris.
4) And (3) filtering and concentrating: filtering with sterile filter membrane, concentrating in an ultrafiltration centrifugal tube, transferring the concentrated solution into an ultracentrifuge tube added with sucrose with the mass concentration of 300g/L, centrifuging, diluting the bottom precipitate with PBS, concentrating again with filter membrane, filtering and sterilizing to obtain the essence stem cell preparation.
5) Mixing: mixing the prepared essence stem cell preparation, sodium hyaluronate, cell growth factor, hyaluronic acid and placenta polypeptide, and stirring to obtain the final product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210864755.0A CN115317433A (en) | 2022-07-21 | 2022-07-21 | Preparation process of stem cell preparation for beautifying and resisting aging |
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| CN202210864755.0A CN115317433A (en) | 2022-07-21 | 2022-07-21 | Preparation process of stem cell preparation for beautifying and resisting aging |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116531485A (en) * | 2023-04-11 | 2023-08-04 | 陕西朗泰生物科技有限公司 | Preparation method of human anti-aging cell substance |
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2022
- 2022-07-21 CN CN202210864755.0A patent/CN115317433A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116531485A (en) * | 2023-04-11 | 2023-08-04 | 陕西朗泰生物科技有限公司 | Preparation method of human anti-aging cell substance |
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