CN115305144A - Method for extracting TG type tuna eye nest oil - Google Patents
Method for extracting TG type tuna eye nest oil Download PDFInfo
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- CN115305144A CN115305144A CN202211025292.5A CN202211025292A CN115305144A CN 115305144 A CN115305144 A CN 115305144A CN 202211025292 A CN202211025292 A CN 202211025292A CN 115305144 A CN115305144 A CN 115305144A
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000000605 extraction Methods 0.000 claims abstract description 92
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000002904 solvent Substances 0.000 claims abstract description 5
- 239000011229 interlayer Substances 0.000 claims description 38
- 210000004279 orbit Anatomy 0.000 claims description 33
- 239000010410 layer Substances 0.000 claims description 32
- 238000003756 stirring Methods 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 15
- 238000002137 ultrasound extraction Methods 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 4
- 235000021122 unsaturated fatty acids Nutrition 0.000 claims description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 claims description 2
- 240000003826 Eichhornia crassipes Species 0.000 claims 2
- 239000000126 substance Substances 0.000 abstract description 3
- 239000006227 byproduct Substances 0.000 abstract description 2
- 235000019198 oils Nutrition 0.000 description 127
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 26
- 238000001816 cooling Methods 0.000 description 13
- 238000010438 heat treatment Methods 0.000 description 13
- 229910052757 nitrogen Inorganic materials 0.000 description 13
- 238000004806 packaging method and process Methods 0.000 description 13
- 238000005086 pumping Methods 0.000 description 13
- 238000007789 sealing Methods 0.000 description 13
- 229910001220 stainless steel Inorganic materials 0.000 description 13
- 239000010935 stainless steel Substances 0.000 description 13
- 238000004321 preservation Methods 0.000 description 12
- 239000012634 fragment Substances 0.000 description 10
- 238000010411 cooking Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 150000002978 peroxides Chemical class 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 235000021323 fish oil Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241000269821 Scombridae Species 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000007434 physicochemical evaluation Methods 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002207 retinal effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
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- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses an extraction method of TG type tuna eye nest oil, which comprises primary extraction and secondary extraction, wherein DHA + EPA can reach 40% by adopting specific temperature and water as a solvent for extraction; the process is simple and easy to operate, the prepared TG type tuna orbital oil is light yellow and clear, has no obvious fishy smell, has good physical and chemical indexes, is rich in natural DHA and EPA, and can realize high-value utilization of tuna byproduct resources.
Description
Technical Field
The invention relates to the technical field of biology, and in particular relates to a method for extracting TG type tuna eye nest oil.
Background
Tuna is an animal belonging to Scombridae of Perciformes, and is a large edible fish of ocean importance. Tuna oil is one of the hot spots of domestic and foreign research for many years because of being rich in n-3 series polyunsaturated fatty acids, DHA and EPA have important health care effects on human bodies. DHA contained in the tuna oil is an essential nutrient for formation and development of brain cells, and has a protective effect on retinal cells; EPA is mainly used for reducing triglyceride and preventing vascular diseases. The fish oil has higher content of effective components and better health care effect.
Most of the traditional fish oil extraction processes are high-temperature cooking or enzymolysis oil extraction, oil oxidation deepens easily caused by high-temperature cooking or long-time enzymolysis, the color is darker, partial nutritional ingredients in the oil are damaged to a certain extent by high temperature, certain denatured foreign proteins are dissolved, certain difficulty is caused for later oil separation, and meanwhile, the oil prepared by high-temperature cooking or enzymolysis oil extraction has unsatisfactory sensory and physical and chemical indexes. In addition, the enzymolysis method has high cost and longer enzymolysis time.
Disclosure of Invention
Aiming at the technical problems in the prior art, the invention aims to provide the preparation method of the TG type tuna eye socket oil with simple extraction process and less solvent.
A method for extracting TG type tuna eye nest oil comprises the following steps:
(1) Cutting eye sockets of the frozen tuna into pieces with sizes and specifications;
(2) Placing minced tuna eye socket into a sandwich extraction tank, adding water as solvent, extracting for one time, and standing for 10-60min for layering;
(3) Removing the water layer, keeping the oil layer, and placing the oil layer into an interlayer extraction tank for secondary extraction;
(4) And (4) performing oil-water residue-liquid separation, adopting a disk centrifuge to separate, and collecting an oil phase, namely the unsaturated fatty acid-rich oil in the golden gun hole nest.
In some embodiments of the invention, the eye sockets in step 1) are cut into pieces of a size of 1 × 1 × 1 to 4 × 4 × 4cm, preferably 2 × 2 × 2 to 4 × 4 × 4cm.
In some embodiments of the invention, the weight ratio of the tuna eye sockets to the water in step 2) is 1:2-1:5.
In some embodiments of the present invention, the first extraction in step 2) is agitation extraction or ultrasonic extraction.
In some embodiments of the present invention, the first extraction in step 2) is stirring extraction, the stirring speed is 200 to 500r/min, the extraction time of the first extraction is 1 to 4 hours, and the temperature of the first extraction is 10 to 30 ℃. In some embodiments of the invention, the one-time extraction in the step 2) is ultrasonic extraction, the frequency of the ultrasonic extraction is 400-600kHz, the extraction time of the one-time extraction is 1-4 h, and the temperature of the one-time extraction is 10-30 ℃.
In some embodiments of the present invention, the temperature of the second extraction in the step 3) is 70 to 85 ℃, and the time of the second extraction is 10 to 60min.
Compared with the prior art, the invention has the following beneficial effects:
the method does not need enzyme preparation or other chemical reagents, adopts pure physical extraction, and has high extraction rate and excellent quality; the TG type tuna nest oil is different from the traditional high-temperature cooking extraction process, and the nest oil extracted by the process is light in color and light in fishy smell; the invention adopts specific temperature and water as solvent to extract, which can make DHA + EPA reach 40%; the process is simple and easy to operate, the prepared TG type tuna eye socket oil is light yellow and clear, has no obvious fishy smell, has good physical and chemical indexes, is rich in natural DHA and EPA, and can realize high-value utilization of tuna byproduct resources.
Detailed Description
The present invention is further illustrated by the following examples, which should not be construed as limiting the scope of the invention. The tuna eye socket: tuna heads were kept at ultra-low temperature and the gill and mouth posterior eye sockets and surrounding fatty tissue were excised.
Example 1
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 2 multiplied by 2cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to the material-liquid ratio of 1:2, starting a stirring paddle, and controlling the stirring speed at 400r/min and the temperature at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 80 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) quickly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 2
A method for extracting TG type tuna eyenest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 2 multiplied by 2cm;
2) Putting the broken blocks of the eye sockets into a sandwich extraction tank, adding pure water according to the material-liquid ratio of 1:2, starting a stirring paddle, and controlling the stirring speed to be 400r/min and the temperature to be 40 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 80 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 3
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 2 multiplied by 2cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to the material-liquid ratio of 1:2, starting a stirring paddle, and controlling the stirring speed at 400r/min and the temperature at 50 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 80 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 4
A method for extracting TG type tuna eyenest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 2 multiplied by 2cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to the material-liquid ratio of 1:2, starting a stirring paddle, and controlling the stirring speed at 400r/min and the temperature at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, removing the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 50 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 5
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 2 multiplied by 2cm;
2) Putting the broken blocks of the eye sockets into a sandwich extraction tank, adding pure water according to the material-liquid ratio of 1:2, starting a stirring paddle, and controlling the stirring speed to be 400r/min and the temperature to be 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 60 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 6
A method for extracting TG type tuna eyenest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 2 multiplied by 2cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to the material-liquid ratio of 1:2, starting a stirring paddle, and controlling the stirring speed at 400r/min and the temperature at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 70 deg.C, and extracting for 20min under heat preservation;
4) Starting a disk centrifuge, pumping the extracting solution obtained in the step 3) into the disk centrifuge, and collecting the separated oil phase; and (3) quickly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 7
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye socket of tuna after frozen storage into 3 × 2 × 4cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to the material-liquid ratio of 1:5, starting a stirring paddle, and controlling the stirring speed at 400r/min and the temperature at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank to steam, heating cold oil in tank to central temperature of 85 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) quickly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 8
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 2 multiplied by 2cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to the material-liquid ratio of 1:2, starting a stirring paddle, and controlling the stirring speed at 400r/min and the temperature at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 90 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 9
A method for extracting TG type tuna eyenest oil comprises the following steps:
1) Cutting eye socket of tuna after frozen storage into pieces with specification and size of 4 × 2 × 2cm;
2) Putting the broken blocks of the eye sockets into a sandwich extraction tank, adding pure water according to the material-liquid ratio of 1:4, starting a stirring paddle, and controlling the stirring speed to be 400r/min and the temperature to be 30 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, removing the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 80 deg.C, and extracting for 20min under heat preservation;
4) Starting a disk centrifuge, pumping the extracting solution obtained in the step 3) into the disk centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 10
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye socket of tuna after frozen storage into pieces with specification and size of 2 × 2 × 4cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to a material-liquid ratio of 1:3, and performing ultrasonic extraction, wherein the ultrasonic frequency is 500kHz, and the temperature can be controlled at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 80 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) quickly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 11
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting the eye of the frozen tuna into pieces to form complete eye tissue;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to a material-liquid ratio of 1:3, and performing ultrasonic extraction, wherein the ultrasonic frequency is 500kHz, and the temperature can be controlled at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 80 deg.C, and extracting for 20min under heat preservation;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 12:
a method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification size of 1 multiplied by 1 cm;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to a material-liquid ratio of 1:3, and performing ultrasonic extraction, wherein the ultrasonic frequency is 500kHz, and the temperature can be controlled at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank, heating the oil in tank to central temperature of 80 deg.C, and extracting for 20min;
4) Starting a disc centrifuge, pumping the extracting solution obtained in the step 3) into the disc centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Example 13
A method for extracting TG type tuna eye nest oil comprises the following steps:
1) Cutting eye sockets of the frozen tuna into pieces with the specification and size;
2) Putting the eye nest fragments into an interlayer extraction tank, adding pure water according to a material-liquid ratio of 1:3, and performing ultrasonic extraction, wherein the ultrasonic frequency is 500kHz, and the temperature can be controlled at 20 +/-2 ℃; extracting for 2h, standing for 30min, opening the discharge port, discarding the lower water solution, and keeping the upper oil layer;
3) Placing the oil layer in interlayer extraction tank, starting interlayer extraction tank steam, heating cold oil in tank to central temperature of 80 deg.C, and extracting for 20min under heat preservation;
4) Starting a disk centrifuge, pumping the extracting solution obtained in the step 3) into the disk centrifuge, and collecting the separated oil phase; and (3) rapidly cooling the separated oil phase to room temperature, filling the oil phase into a 50L food-grade stainless steel barrel, filling nitrogen, packaging, sealing by a cover, and storing in a shady, dry and ventilated place to obtain the TG type tuna eye nest oil.
Effect application example 1:
the following effect data were tested for examples 1-13: the test results of the color, smell, peroxide value, acid value, extraction rate and DHA + EPA content of the pit oil are shown in Table 1.
TABLE 1 sensory and physicochemical evaluation
| Item | Color | Fishy smell | Peroxide value meq/Kg | Acid value mg/g | The extraction rate% | DHA + EPA content% |
| Example 1 | Light yellow | Is relatively light | 4.0 | 0.41 | 97 | 40 |
| Examples2 | Is deeper | Is slightly provided with | 9.2 | 0.48 | 90 | 30 |
| Example 3 | Is deeper | Is slightly provided with | 9.7 | 0.51 | 93 | 29 |
| Example 4 | Is deeper | Is slightly provided with | 5.1 | 0.55 | 90 | 30 |
| Example 5 | Is deeper | Is slightly provided with | 5.2 | 0.51 | 91 | 31 |
| Example 6 | Is deeper | Is slightly provided with | 4.8 | 0.52 | 90 | 32 |
| Example 7 | Light yellow | Is relatively light | 4.1 | 0.40 | 97 | 39 |
| Example 8 | Is deeper | Is slightly provided with | 5.3 | 0.56 | 89 | 29 |
| Example 9 | Light yellow | Is relatively light | 4.0 | 0.42 | 96 | 39 |
| Example 10 | Light yellow | Is relatively light | 4.0 | 0.41 | 98 | 41 |
| Example 11 | Is deeper | Is slightly provided with | 5.3 | 0.57 | 85 | 29 |
| Example 12 | Is deeper | Is slightly provided with | 5.6 | 0.49 | 85 | 25 |
| Example 13 | Is deeper | Is slightly provided with | 5.8 | 0.67 | 83 | 20 |
From table 1, it can be seen that the temperature of the primary extraction and the secondary extraction has great influence on the final yield, the color and the physicochemical properties of the product, and the ultrasonic extraction adopted in the primary extraction has higher extraction rate than the stirring extraction; too high a temperature in one extraction will result in a darker color and a higher peroxide value of the tuna eye nest oil. The tissues are large and are not broken, so that the grease cannot be fully dissolved out; the tissue is too fine and easy to form an emulsified state under the action of stirring with certain intensity, so that the oil contained in the crushed impurities cannot be dissolved out and is difficult to separate, and therefore, the eye socket raw material with a proper size can obtain a high extraction rate and the eye socket oil rich in EPA + DHA.
While the invention has been described with reference to specific embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. A TG type tuna eye nest oil extraction method is characterized by comprising the following steps:
(1) Cutting eye sockets of the frozen tuna into pieces with sizes and specifications;
(2) Placing minced tuna eye socket into a sandwich extraction tank, adding water as solvent, extracting for one time, and standing for 10-60min for layering;
(3) Removing the water layer, keeping the oil layer, and placing the oil layer into an interlayer extraction tank for secondary extraction;
(4) And (4) carrying out oil-water residue-liquid separation, adopting a disc centrifuge for separation, and collecting an oil phase, namely the golden gun eye nest oil rich in unsaturated fatty acid.
2. The method for extracting oil from the eye sockets of tuna in the TG type according to claim 1, wherein the eye sockets in step 1) are cut into pieces having a size of 1 x 1 to 4 x 4cm, preferably 2 x 2 to 4 x 4cm.
3. The method for extracting oil from tuna eye sockets of the TG type according to claim 1, wherein the weight ratio of tuna eye sockets to water in step 2) is 1:2-1:5.
4. The method for extracting TG type tuna eye socket oil according to claim 1, wherein the primary extraction in the step 2) is stirring extraction or ultrasonic extraction.
5. The method for extracting oil from Eichhornia crassipes cells of TG type according to claim 3, wherein the primary extraction in step 2) is stirring extraction, the stirring speed is 200-500 r/min, the extraction time of the primary extraction is 1-4 h, and the temperature of the primary extraction is 10-30 ℃.
6. The method for extracting oil from Eichhornia crassipes cav of TG type as claimed in claim 3, wherein the primary extraction in step 2) is ultrasonic extraction, the frequency of the ultrasonic extraction is 400-600kHz, the extraction time of the primary extraction is 1-4 h, and the temperature of the primary extraction is 10-30 ℃.
7. The method for extracting TG type tuna eye socket oil according to claim 1, wherein the temperature of the secondary extraction in the step 3) is 70-85 ℃, and the time of the secondary extraction is 10-60 min.
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