CN115299543B - Solid beverage for promoting human dopamine anabolism and preparation method thereof - Google Patents

Solid beverage for promoting human dopamine anabolism and preparation method thereof Download PDF

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CN115299543B
CN115299543B CN202210968100.8A CN202210968100A CN115299543B CN 115299543 B CN115299543 B CN 115299543B CN 202210968100 A CN202210968100 A CN 202210968100A CN 115299543 B CN115299543 B CN 115299543B
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powder
solid beverage
anabolism
red date
dopamine
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CN115299543A (en
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楼秀余
吴锦青
楼圣婷
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Shanghai Shengyue Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385Concentrates of non-alcoholic beverages
    • A23L2/39Dry compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/175Amino acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The application relates to the technical field of solid beverage preparation, and particularly discloses a solid beverage for promoting human dopamine anabolism and a preparation method thereof. A solid beverage for promoting dopamine anabolism of human body is prepared from the following raw materials: red date slow release powder, protein peptide premix, yolk globular protein powder, amino acid premix, sweetener and wetting agent; the preparation method of the red date slow-release powder comprises the following steps: adding red date powder, ginger powder and hydroxypropyl methylcellulose into chitosan hydrochloride solution, and stirring and mixing to obtain a mixture A; adding glyceraldehyde into the mixture A, stirring and mixing, filtering and discarding the filtrate, drying, crushing, and sieving with a 80-100 mesh sieve to obtain the red date slow-release powder. The solid beverage for promoting the anabolism of the dopamine in the human body can promote the activity of tyrosine hydroxylase and promote the anabolism of the dopamine.

Description

Solid beverage for promoting human dopamine anabolism and preparation method thereof
Technical Field
The application relates to the technical field of solid beverage preparation, in particular to a solid beverage for promoting human dopamine anabolism and a preparation method thereof.
Background
Dopamine (DA for short) is a central neurotransmitter synthesized by dopaminergic neurons and stored in vesicles, which are released by neurons to assist cells in delivering impulses. The central neurotransmitter directly affects the emotion of a person and has the effects of regulating body swing, mental activities, endocrine and vascular activities.
The anabolic processes of dopamine are: tyrosine in blood is taken up by dopaminergic neurons, catalyzed by Tyrosine Hydroxylase (TH) to become L-dopa, and DA is generated under the action of Dopa Decarboxylase (DDC).
In the life of people, solid beverages are increasingly appearing, and are spread throughout the life of people due to their excellent portability relative to liquid beverages. Tyrosine is a common technical means to increase the concentration of DA in blood after eating.
In the related art, a solid beverage mixture comprises 15% of tyrosine, 15% of maca powder, 15% of taurine, 8% of cocoa extract, 6% of cinnamaldehyde oil-embedding powder, 6% of moringa seed powder, 6% of curcumin, 6% of menthol oil-embedding powder, 6% of wormwood extract, 16% of ginsenoside Rg, 4% of macamide, 4% of guarana extract and 6% of coffee bean extract, wherein more tyrosine is added for improving DA concentration in blood.
With respect to the above-mentioned related art, the inventors found that the ability of eating tyrosine to raise the concentration of DA is limited because the content and activity of TH in dopaminergic neurons is only 0.1-1% of DDC, and thus TH can be regarded as a rate-limiting factor for DA synthesis, and when TH activity is low, it may be ineffective or nonfunctional, and the effect of enhancing dopamine anabolism is very limited. Therefore, it is of great importance to develop a solid beverage capable of improving TH activity.
Disclosure of Invention
In order to improve activity of TH and promote anabolism of human dopamine, the application provides a solid beverage for promoting anabolism of human dopamine and a preparation method thereof.
In a first aspect, the present application provides a solid beverage for promoting dopamine anabolism in a human body, which adopts the following technical scheme:
a solid beverage for promoting dopamine anabolism of a human body, which comprises the following components in parts by weight:
200-300 parts of red date slow-release powder;
70-150 parts of protein peptide premix;
10-20 parts of egg yolk globulin powder;
5-50 parts of amino acid premix;
20-60 parts of sweetener;
50-100 parts of a wetting agent;
the preparation method of the red date slow-release powder comprises the following steps: adding red date powder, ginger powder and hydroxypropyl methylcellulose into chitosan hydrochloride solution, and stirring and mixing to obtain a mixture A; adding glyceraldehyde into the mixture A, stirring and mixing, filtering and discarding the filtrate, drying, crushing, and sieving with a 80-100 mesh sieve to obtain the red date slow-release powder.
By adopting the technical scheme, the red jujube powder is rich in a large amount of cyclic adenosine monophosphate (cAMP for short), and the phosphorylation of the cAMP can improve the activity of tyrosine hydroxylase (TH for short), so that the anabolism of dopamine (DA for short) is promoted. Gingerol in ginger powder has effects of inhibiting body inflammation, reducing "leptin resistance" caused by body inflammation, promoting body utilization of leptin and reducing leptin concentration in blood. The leptin concentration in blood is inversely related to the activity of TH, so that the activity of TH can be further improved by adding ginger powder, and the anabolism of DA can be promoted.
Meanwhile, chitosan is used as a carrier of the red date powder and the ginger powder, so that a slow release effect is achieved, the release time of beneficial substances in the red date powder and the ginger powder in a human body is prolonged, the duration of the effect of promoting TH activity caused by cAMP and gingerol in the ginger powder is prolonged, and the anabolism of DA is further promoted. In addition, the ginger powder is coated by the chitosan, so that the pungent taste in the solid beverage is reduced, and the flavor and the taste of the solid beverage are improved.
The solid beverage prepared by the application is subjected to animal serum dopamine content level experiments, and the DA concentration of the solid beverage is as low as 15.6 mug/g, which is higher than that of the solid beverage prepared by the red date slow-release powder which is not used, and 11.4 mug/g, which shows that the solid beverage added with the red date powder and the ginger powder can improve the DA concentration and promote the anabolism of DA; and in-vitro digestion simulation experiments are carried out, and the time for the cAMP and total phenol content of the solution to reach 90% of the initial content is minimally increased to 50min, so that the duration of the effect of promoting TH activity can be prolonged by adding the solid drink of chitosan.
Preferably, the weight ratio of the red date powder, the ginger powder and the chitosan hydrochloride solution is as follows: 1 (0.5-2) is (10-20); the concentration of the chitosan hydrochloride solution is 1-3wt%.
By adopting the technical scheme, when the weight ratio of the red date powder, the ginger powder and the chitosan hydrochloride solution is in the range, the compounding effect of the red date powder, the ginger powder and the chitosan hydrochloride solution is better, the solid beverage prepared by the application is subjected to animal serum dopamine content level experiments and in-vitro digestion simulation experiments, the DA concentration is improved to 18.4-19.3 mug/g, and the time for the cAMP and the total phenol content of the solution to reach 90% of the initial content is improved to 60min.
Preferably, the chitosan hydrochloride solution is prepared from modified chitosan, water, ethanol and hydrochloric acid; the preparation method of the modified chitosan comprises the following steps: adding chitosan into potassium chloride aqueous solution, heating, stirring and mixing to obtain a mixture B, adding genipin into the mixture B, stirring and mixing, standing, filtering and discarding filtrate, drying, pulverizing, and sieving with 200-500 mesh sieve to obtain modified chitosan.
Through adopting above-mentioned technical scheme, carry out animal serum dopamine content level experiment and external digestion simulation experiment with the solid beverage that this application made, its DA concentration promotes to 20.5 mu g/g from 19.3 mu g/g, and solution cAMP reaches the time of 90% of each initial content to 70min from 60min, promotes the anabolism effect of DA better after eating the solid beverage, and analysis its probably cause lies in:
in the chemical crosslinking process of chitosan and genipin, the potassium chloride can occupy part of the positions of the polymer, and then the potassium chloride is hydrolyzed and removed, so that a large number of micropores are formed on the surface of the polymer, the amount of chitosan loaded red date powder and ginger powder is increased, and a better slow release effect is achieved.
In addition, the potassium chloride is used as a common mineral water additive, and trace potassium chloride which is not removed by hydrolysis can supplement electrolyte of a human body after being taken.
Preferably, the weight ratio of the chitosan to the potassium chloride aqueous solution is 1:15-20.
By adopting the technical scheme, when the weight ratio of the chitosan to the potassium chloride aqueous solution is in the range, the compounding effect of the chitosan and the potassium chloride aqueous solution is better, and the anabolism effect of promoting DA after eating the solid beverage is better. The solid beverage prepared by the application is subjected to animal serum dopamine content level experiments and in-vitro digestion simulation experiments, the DA concentration is further improved to 21-21.5 mug/g, and the time for the cAMP and total phenol content of the solution to reach 90% of the initial content is further improved to 80min.
Preferably, the concentration of the potassium chloride aqueous solution is 0.1-0.5g/mL.
By adopting the technical scheme, when the concentration of the potassium chloride aqueous solution is in the range, the concentration of the potassium chloride aqueous solution is more suitable, and the chitosan surface is possibly promoted to generate more micropores, so that the amounts of the red date powder and the ginger powder loaded by the chitosan are more, and the anabolism effect of promoting DA is better after the solid beverage is eaten. The solid beverage prepared by the application is subjected to animal serum dopamine content level experiments and in-vitro digestion simulation experiments, the DA concentration is further improved to 21.9-22.3 mug/g, and the time for the cAMP and total phenol content of the solution to reach 90% of the initial content is further improved to 90min.
Preferably, maltodextrin is also added during the addition of glyceraldehyde to mixture a.
By adopting the technical scheme, the maltodextrin has good heat resistance, improves the stability of the red date powder and the ginger powder, reduces the viscosity of the red date powder and the ginger powder during drying, and reduces the occurrence of the condition that the red date slow-release powder adheres to the wall during the drying process.
Preferably, the protein peptide premix is one or more of medicinal peptide, bovine bone collagen peptide, tuna oligopeptide powder, coix seed oligopeptide powder, bovine heart peptide, bovine liver peptide, bovine spleen peptide, sheep lung peptide, bovine kidney peptide, sea cucumber peptide, ginseng peptide and oyster oligopeptide powder.
By adopting the technical scheme, during actual production, proper protein peptide additives can be selected according to the needs so as to meet different functional needs.
Preferably, the amino acid premix is a mixture of one or more of L-arginine hydrochloride, L-glutamic acid, L-lysine hydrochloride, L-aspartic acid, glycine, N-acetylneuraminic acid and L-glutamine.
By adopting the technical scheme, during actual production, proper amino acid additives can be selected according to the needs so as to meet different functional needs.
Preferably, the sweetener is one or more of sucralose, erythritol, aspartame, acesulfame potassium and steviol glycoside.
By adopting the technical scheme, the sweetness of the solid beverage is improved, the glucose concentration in blood is not influenced, the physical burden is reduced, and the solid beverage can be drunk for a long time, so that the solid beverage has a wide application prospect.
In a second aspect, the present application provides a method for preparing a solid beverage for promoting dopamine anabolism in a human body, which adopts the following technical scheme:
a method for preparing the solid beverage for promoting dopamine anabolism in human body according to claims 1-9, comprising the following steps: s1, mixing red date slow-release powder, a protein peptide premix, yolk globulin powder, an amino acid premix and a sweetener to obtain a mixture C;
s2, adding a wetting agent into the mixture C, and stirring and mixing to obtain a mixture D;
s3, granulating the mixture D, and drying at 10-20 ℃ to obtain the solid beverage for promoting the synthesis and metabolism of the dopamine in the human body.
By adopting the technical scheme, the components of the solid beverage are directly mixed, so that the method is direct and convenient, and the efficiency in industrial production can be improved.
In summary, the present application has the following beneficial effects:
1. according to the method, the TH activity is directly or indirectly improved by using the red date powder and the ginger powder, so that the anabolism of DA is promoted, and the slow release effect is achieved by using chitosan to load the red date powder and the ginger powder, so that the action time of improving the TH activity by cAMP and gingerol is prolonged, and the anabolism effect of DA is better;
2. in the application, potassium chloride is used for modifying chitosan, so that micropores are formed on the surface of the chitosan, the amount of chitosan loaded with red date powder and ginger powder is increased, and the anabolism effect of DA is further improved;
3. the method is convenient for processing and production, and the prepared solid beverage has higher portability.
Detailed Description
The present application is described in further detail below with reference to examples.
The raw materials used in the examples of the present application are commercially available except for the following specific descriptions:
red date powder: cAMP content of 371. Mu.g/g;
ginger powder: the total phenol content is 8.54mg/g, and gingerol mainly comprises 6-gingerol, 8-gingerol and 10-gingerol;
all raw material additive dosages in the embodiment of the application meet the use amount specified in GB 2760-2011 food additive use Standard.
Preparation example
Preparation example 1
A red date slow-release powder is prepared by the following steps: adding 1kg of red date powder, 0.4kg of ginger powder and 0.2kg of hydroxypropyl methyl cellulose into 8kg of chitosan hydrochloride solution, stirring and mixing, and reacting for 2 hours to obtain a mixture A; adding 0.5kg of glyceraldehyde into the mixture A, stirring and mixing, reacting for 3 hours, filtering and discarding the filtrate, drying, crushing and sieving with a 80-mesh sieve to obtain the red date slow-release powder;
the preparation method of the chitosan hydrochloride solution comprises the following steps: mixing chitosan, water, ethanol and hydrochloric acid with the concentration of 31wt percent uniformly according to the weight ratio of 0.8:60:45:0.2 (the concentration is 0.75wt percent), and obtaining chitosan hydrochloride solution, wherein the chitosan deacetylation degree is 80 percent and the water solubility is better;
wherein, spray drying is adopted for drying, the technological parameters are that the air inlet temperature is 160 ℃, and the air outlet temperature is 100 ℃.
PREPARATION EXAMPLES 2 to 4
The red date slow-release powder is different from the preparation example 1 in that the weight ratio of red date powder, ginger powder and chitosan hydrochloride solution is different, and is specifically shown in table 1.
Preparation examples 5 to 7
The red date slow-release powder is different from the preparation example 3 in that the concentration of chitosan hydrochloride solution is different, and is shown in table 1.
TABLE 1 parameters of the components in preparation examples 1 to 7
Preparation example 8
The red date slow-release powder is different from preparation example 6 in that an equivalent amount of modified chitosan is used for replacing chitosan, wherein the preparation method of the modified chitosan is as follows:
adding 1kg of chitosan into 25kg of potassium chloride aqueous solution with the concentration of 0.6g/mL, heating to 60 ℃, stirring and mixing, and reacting for 1h to obtain a mixture B; adding 0.6kg of genipin into the mixture B, stirring and mixing, reacting for 3 hours, standing for 1 hour, filtering and discarding filtrate, vacuum drying for 2 hours, crushing, and sieving with a 300-mesh sieve to obtain the modified chitosan.
Preparation examples 9 to 11
The red date slow-release powder is different from preparation example 8 in that the weight ratio of chitosan to potassium chloride aqueous solution is different, and is shown in table 2.
Preparation examples 12 to 14
The red date slow-release powder is different from preparation example 10 in the concentration of potassium chloride aqueous solution, and is shown in table 2.
TABLE 2 preparation examples 8-14 Components and weights (kg)
Preparation example 15
The red date slow-release powder is different from preparation example 13 in that maltodextrin with a weight ratio of 1:1 with chitosan is also added in the process of adding glyceraldehyde into the mixture A.
Examples
Example 1
The solid beverage for promoting the anabolism of the human body dopamine comprises the following components in parts by weight as shown in table 3, and is prepared by the following steps:
s1, mixing components except a wetting agent according to a table 3 to obtain a mixture C;
s2, adding 50kg of wetting agent into the mixture C, stirring and mixing, and reacting for 20min to obtain a mixture D, wherein the wetting agent is edible alcohol with the concentration of 70 wt%;
s3, granulating the mixture D, and drying at 20 ℃ for 5 hours to obtain the solid beverage for promoting the synthesis and metabolism of the dopamine in the human body;
wherein the red date slow-release powder is prepared from preparation example 1.
Examples 2 to 5
A solid beverage for promoting dopamine anabolism in human body is different from example 1 in that the amounts of each component used are different, specifically as shown in Table 3.
TABLE 3 weight (kg) of the components in examples 1-5
Wherein the protein peptide premix is medicinal peptide, bovine bone collagen peptide, tuna oligopeptide powder, coicis semen oligopeptide powder, bovine heart peptide, bovine liver peptide, bovine spleen peptide, sheep lung peptide, bovine kidney peptide, sea cucumber peptide, ginseng radix peptide, and oyster oligopeptide powder; it should be noted that, in other embodiments, different protein peptides may be added according to actual production requirements, so as to meet different functional requirements, and not affect the anabolism of DA.
The amino acid premix is L-arginine hydrochloride, L-glutamic acid, L-lysine hydrochloride, L-aspartic acid, glycine, N-acetylneuraminic acid, and L-glutamine; it should be noted that, in other embodiments, different amino acids may be selected and added according to actual production requirements, so as to meet different functional requirements, and not affect the anabolism of DA.
The sweetener is steviol glycoside with water content of 8%. In other embodiments, according to actual production requirements, a sweetener which is not absorbed by human body, such as sucralose, erythritol, aspartame, acesulfame potassium, etc., may be added, without affecting the anabolism of DA.
Examples 6 to 19
The solid beverage for promoting dopamine anabolism of human body is different from example 1 in that the use condition of the red date slow-release powder is different, and is specifically shown in table 4.
Comparative example
Comparative example 1
A solid beverage differs from example 1 in that an equivalent amount of soy protein powder is used instead of the red date slow release powder.
Comparative example 2
A solid beverage differs from example 1 in that the same amount of red date powder is used instead of the red date slow-release powder.
Comparative examples 3 to 4
A solid beverage was different from example 1 in the amounts of the components used, as shown in Table 5.
TABLE 5 Components of example 1, comparative examples 1-4 and weights (kg)
Performance detection
The solid beverages prepared in examples and comparative examples were subjected to the following test, and the test results are shown in table 6.
Experiment one, animal serum dopamine content level experiment: dissolving solid beverage in drinking water at concentration of 4wt%, and storing;
blank blood collection: collecting 0.5mL of venous blood of a mouse which is not fed for 12 hours, adding an anticoagulant, and preserving at a low temperature for later use;
and (5) collecting after eating: feeding the mice with the drinking water containing the solid beverage 1 hour after collecting blank blood, collecting 0.5mL of blood sample after eating for 30min, and centrifuging to obtain serum;
the concentration of dopamine in serum is detected by adopting a fluorescence spectrophotometry method, and the unit is mug/g.
Experiment two, in vitro digestion simulation experiment: dissolving solid beverage in artificial gastric juice at concentration of 0.25wt%, shaking at 37deg.C, taking out 1mL of solution every 10min, and detecting time when cAMP and total phenol content in the solution reach 90% (i.e. cAMP reaches 334 μg/g and total phenol reaches 7.69 mg/g) or more;
preparation of artificial gastric juice: diluting 23.4mL of concentrated hydrochloric acid to 100mL, taking 1.64mL again, dissolving 100mg of pepsin to 4g/L, taking out 30mL, adjusting pH to 2, adding 1g of sodium chloride for dissolution, and obtaining artificial gastric juice, and preserving at 4 ℃ for later use;
the taken solution is detected by a high performance liquid chromatograph under the following detection conditions: the column temperature is 35 ℃, the ultraviolet wavelength is 280nm, the flow rate of the mobile phase is 1mL/min, the sample injection amount is 10 mu L, and the mobile phase is acetonitrile and water.
TABLE 6 Performance test results for examples 1-19, comparative examples 1-4
The properties of the solid beverages prepared in the present application were analyzed in the following manner in combination with the corresponding data in examples 1 to 19, comparative examples 1 to 4 and Table 6.
As can be seen from table 6, in comparative example 1, the concentration of dopamine (abbreviated as DA) in the serum of mice was 11.4 μg/g, and the level of dopamine was in a steady state by drinking the solid beverage prepared by replacing the red date slow-release powder with an equal amount of soybean protein powder; in comparative example 2, the DA concentration in the serum of mice was 13.1. Mu.g/g by drinking an equal amount of red date powder instead of the red date slow-release powder, and the time taken for the cAMP and total phenol contents of the solutions to reach 90% of the respective initial contents was measured to be 30min.
In example 1, however, the concentration of DA in serum after drinking of mice was 15.6. Mu.g/g, and the time taken for the cAMP and total phenol content of the solution to reach 90% of the respective initial contents was measured to be 50min, because of using the solid drink prepared from the red date slow-release powder prepared in preparation example 1.
Compared with comparative example 1, the concentration of DA in example 1 is greatly improved, which indicates that the solid beverage prepared by the application is probably due to the addition of the red date powder and the ginger powder, and the anabolism of DA is promoted; compared with comparative example 2, the time for the solution cAMP and total phenol content in example 1 to reach 90% of the initial content respectively is prolonged from 30min to 50min, and the DA concentration is also improved, which indicates that the red date powder and the ginger powder are loaded and have a slow release effect probably due to the addition of chitosan, and the duration of the effect of promoting TH activity in human bodies of cAMP in the red date powder and gingerol in the ginger powder is prolonged, so that the anabolism of DA is further promoted.
Examples 2 to 5 and comparative examples 3 to 4 differ from example 1 in the amounts of the respective components used. The DA concentration in examples 1-5 was 15.6-16.9. Mu.g/g, and the time taken for the cAMP and total phenol content of the solutions to reach 90% of the respective initial contents was 50min, whereas the DA concentration in comparative examples 3-4 was 14.9-15.2. Mu.g/g, and the time taken for the cAMP and total phenol content of the solutions to reach 90% of the respective initial contents was only 40min, which were lower than those in examples 1-5, indicating that the effect of promoting DA anabolism was better when the contents of the components in the solid beverage were in the range of examples 1-5.
Examples 6-8 differ from example 3 in that the weight ratio of red date powder, ginger extract and chitosan hydrochloride solution is different in the preparation process of red date slow-release powder, and the DA concentration in examples 6-8 is higher than that in example 3. And the DA concentration was highest at 17.9 μg/g when the weight ratio of red date powder, ginger extract and chitosan hydrochloride solution was 1:1:15 (example 7).
Examples 9-11 are different from example 7 in that the chitosan hydrochloride concentration is different in the preparation process of the red date slow-release powder, and the time for the solution cAMP and total phenol content in examples 9-11 to reach 90% of the initial content respectively reaches 60min, which is higher than 50min in example 6, which shows that the effect of prolonging and improving TH activity is better when the chitosan hydrochloride concentration is in the range of 1-3wt%.
Example 12 is different from example 10 in that in the preparation process of the red date slow-release powder, the red date slow-release powder is prepared by using modified chitosan instead of chitosan in preparation example 8, so that the DA concentration in serum reaches 20.5 mug/g after a mouse drinks a solid beverage, the time for the cAMP content and the total phenol content of the solution to reach 90% of the initial content respectively reaches 70min, and compared with example 10, the analysis is possible because the potassium chloride is used to cause more micropores on the surface of the chitosan, the amount of the chitosan loaded red date powder and the ginger powder is improved, and the effect of improving TH activity is better.
Examples 13-15 differ from example 12 in that the weight ratio of chitosan to aqueous potassium chloride solution is different during the preparation of the red date slow-release powder. The DA concentration in examples 13-15 reached 21.0-21.5. Mu.g/g, the cAMP and total phenol content in the solution reached 90% of the respective initial levels for 80min, and the data were all superior to example 10, indicating that it was optimal when the weight ratio of chitosan to aqueous potassium chloride solution was 1:18 (example 12).
Examples 16-18 differ from example 14 in the concentration of the aqueous potassium chloride solution during the preparation of the slow-release powder of red dates. The time for the DA concentration, solution cAMP, and total phenol content to reach 90% of the respective initial contents in examples 14 to 16 was further increased compared to example 14, especially from 80min to 90min, indicating that the solid beverage prolonged the TH activity longer when the concentration of the aqueous potassium chloride solution was 0.1 to 0.5g/mL.
Example 19 differs from example 17 in that maltodextrin was also added during the process of adding glyceraldehyde to mixture a during the preparation of the red date slow release powder. The occurrence of wall sticking of the red date powder and the ginger powder in the drying process is reduced, and the sufficient effective components of the red date powder and the ginger powder in the solid beverage are ensured to the greatest extent.
The present embodiment is only illustrative of the present application and is not intended to be limiting, and modifications may be made to the embodiment by those skilled in the art without creative contribution as needed after reading the present specification, but are protected by patent laws within the scope of the claims of the present application.

Claims (8)

1. The solid beverage for promoting the anabolism of the dopamine in the human body is characterized by comprising the following raw materials in parts by weight:
200-300 parts of red date slow-release powder;
70-150 parts of protein peptide premix;
10-20 parts of egg yolk globulin powder;
5-50 parts of amino acid premix;
20-60 parts of sweetener;
50-100 parts of a wetting agent;
the preparation method of the red date slow-release powder comprises the following steps: adding red date powder, ginger powder and hydroxypropyl methylcellulose into chitosan hydrochloride solution, and stirring and mixing to obtain a mixture A; adding glyceraldehyde into the mixture A, stirring and mixing, filtering and discarding the filtrate, drying, crushing, and sieving with a 80-100 mesh sieve to obtain the red date slow-release powder;
the weight ratio of the red date powder to the ginger powder to the chitosan hydrochloride solution is 1 (0.5-2): 10-20; the concentration of the chitosan hydrochloride solution is 1-3wt%;
the preparation raw materials of the chitosan hydrochloride solution comprise modified chitosan, water, ethanol and hydrochloric acid;
the preparation method of the modified chitosan comprises the following steps: adding chitosan into potassium chloride aqueous solution, heating, stirring and mixing to obtain a mixture B, adding genipin into the mixture B, stirring and mixing, standing, filtering and discarding filtrate, drying, pulverizing, and sieving with 200-500 mesh sieve to obtain modified chitosan.
2. A solid beverage for promoting dopamine anabolism in a human as recited in claim 1, wherein: the weight ratio of the chitosan to the potassium chloride aqueous solution is 1:15-20.
3. A solid beverage for promoting dopamine anabolism in a human as recited in claim 1, wherein: the concentration of the potassium chloride aqueous solution is 0.1-0.5g/mL.
4. A solid beverage for promoting dopamine anabolism in a human as recited in claim 1, wherein: during the addition of glyceraldehyde to mixture a, maltodextrin was also added.
5. A solid beverage for promoting dopamine anabolism in a human as recited in claim 1, wherein: the protein peptide premix is one or more of bovine bone collagen peptide, tuna oligopeptide powder, coix seed oligopeptide powder, bovine heart peptide, bovine liver peptide, bovine spleen peptide, sheep lung peptide, bovine kidney peptide, sea cucumber peptide, ginseng peptide and oyster oligopeptide powder.
6. A solid beverage for promoting dopamine anabolism in a human as recited in claim 1, wherein: the amino acid premix is a mixture composed of one or more of L-arginine hydrochloride, L-glutamic acid, L-lysine hydrochloride, L-aspartic acid, glycine, N-acetylneuraminic acid and L-glutamine.
7. A solid beverage for promoting dopamine anabolism in a human as recited in claim 1, wherein: the sweetener is one or more of sucralose, erythritol, aspartame, acesulfame potassium and steviol glycoside.
8. A method for preparing a solid beverage for promoting dopamine anabolism in a human body according to any one of claims 1 to 7, comprising the steps of:
s1, mixing red date slow-release powder, a protein peptide premix, yolk globulin powder, an amino acid premix and a sweetener to obtain a mixture C;
s2, adding a wetting agent into the mixture C, and stirring and mixing to obtain a mixture D;
s3, granulating the mixture D, and drying at 10-20 ℃ to obtain the solid beverage for promoting the synthesis and metabolism of the dopamine in the human body.
CN202210968100.8A 2022-08-12 2022-08-12 Solid beverage for promoting human dopamine anabolism and preparation method thereof Active CN115299543B (en)

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