CN115298323A - 靶向测序方法 - Google Patents

靶向测序方法 Download PDF

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Publication number
CN115298323A
CN115298323A CN202180022918.4A CN202180022918A CN115298323A CN 115298323 A CN115298323 A CN 115298323A CN 202180022918 A CN202180022918 A CN 202180022918A CN 115298323 A CN115298323 A CN 115298323A
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nucleic acid
sequence
target
cases
dna
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CN202180022918.4A
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Chinese (zh)
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基斯·布朗
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Capcod Genomics
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Capcod Genomics
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180022918.4A 2020-01-17 2021-01-15 靶向测序方法 Pending CN115298323A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202062962777P 2020-01-17 2020-01-17
US62/962,777 2020-01-17
US202063009603P 2020-04-14 2020-04-14
US63/009,603 2020-04-14
PCT/US2021/013598 WO2021146534A1 (en) 2020-01-17 2021-01-15 Methods of targeted sequencing

Publications (1)

Publication Number Publication Date
CN115298323A true CN115298323A (zh) 2022-11-04

Family

ID=76864824

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180022918.4A Pending CN115298323A (zh) 2020-01-17 2021-01-15 靶向测序方法

Country Status (7)

Country Link
US (1) US20230056763A1 (https=)
EP (2) EP4592386A3 (https=)
JP (1) JP2023519782A (https=)
CN (1) CN115298323A (https=)
AU (1) AU2021209101A1 (https=)
CA (1) CA3168144A1 (https=)
WO (1) WO2021146534A1 (https=)

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US20230167487A2 (en) 2021-07-12 2023-06-01 Labsimply, Inc. Crispr cascade assay
US20230279375A1 (en) 2021-12-13 2023-09-07 Labsimply, Inc. Signal boost cascade assay
WO2023114052A1 (en) * 2021-12-13 2023-06-22 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
EP4463567A4 (en) * 2022-01-12 2026-02-18 Jumpcode Genomics Inc METHODS AND COMPOSITIONS FOR TRANSCRIPTOME ANALYSIS
WO2024073114A1 (en) 2022-09-30 2024-04-04 Vedabio, Inc. Delivery of therapeutics in vivo via a crispr-based cascade system
US11982677B2 (en) 2022-10-02 2024-05-14 Vedabio, Inc. Dimerization screening assays
US11965205B1 (en) 2022-10-14 2024-04-23 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
WO2024253707A2 (en) 2023-01-07 2024-12-12 Vedabio, Inc. Engineered nucleic acid-guided nucleases
US12060602B2 (en) 2023-01-10 2024-08-13 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification

Citations (3)

* Cited by examiner, † Cited by third party
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US20170247689A1 (en) * 2014-09-09 2017-08-31 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
CN107190008A (zh) * 2017-07-19 2017-09-22 苏州吉赛基因测序科技有限公司 一种基于Crispr/cas9的捕获基因组目标序列的方法及其在高通量测序中的应用
WO2019178577A1 (en) * 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations

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GB0228614D0 (en) * 2002-12-07 2003-01-15 Fu Guoliang Oligonucleotide guided analysis of gene expression
US10174368B2 (en) * 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
JP5839790B2 (ja) * 2010-09-28 2016-01-06 国立大学法人 東京大学 Ｄｎａマイクロアレイの製造方法、ｄｎａマイクロアレイ、タンパク質アレイの製造方法、タンパク質アレイ、及び機能性タンパク質の同定方法
US20160033504A1 (en) * 2013-03-15 2016-02-04 Alder Biopharmaceuticals, Inc. Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens
WO2016100955A2 (en) * 2014-12-20 2016-06-23 Identifygenomics, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
US20170101674A1 (en) * 2015-08-21 2017-04-13 Toma Biosciences, Inc. Methods, compositions, and kits for nucleic acid analysis
DK3455372T3 (da) * 2016-05-11 2020-01-06 Illumina Inc Polynukleotidberigelse og -amplifikation ved anvendelse af argonautsystemer
US10301677B2 (en) * 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10370701B2 (en) * 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
US10907204B2 (en) * 2016-07-12 2021-02-02 Roche Sequencing Solutions, Inc. Primer extension target enrichment
US11414701B2 (en) * 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170247689A1 (en) * 2014-09-09 2017-08-31 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
CN107190008A (zh) * 2017-07-19 2017-09-22 苏州吉赛基因测序科技有限公司 一种基于Crispr/cas9的捕获基因组目标序列的方法及其在高通量测序中的应用
WO2019178577A1 (en) * 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations

Also Published As

Publication number Publication date
US20230056763A1 (en) 2023-02-23
EP4090766B1 (en) 2025-05-21
EP4592386A2 (en) 2025-07-30
EP4090766A1 (en) 2022-11-23
CA3168144A1 (en) 2021-07-22
AU2021209101A1 (en) 2022-08-18
EP4592386A3 (en) 2025-10-15
JP2023519782A (ja) 2023-05-15
WO2021146534A1 (en) 2021-07-22
EP4090766A4 (en) 2024-03-06

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