CN115287366A - 15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application - Google Patents

15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application Download PDF

Info

Publication number
CN115287366A
CN115287366A CN202210732446.8A CN202210732446A CN115287366A CN 115287366 A CN115287366 A CN 115287366A CN 202210732446 A CN202210732446 A CN 202210732446A CN 115287366 A CN115287366 A CN 115287366A
Authority
CN
China
Prior art keywords
chicken
snp
sequencing
breeding
genome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202210732446.8A
Other languages
Chinese (zh)
Inventor
王宇哲
胡晓湘
朱迪
李宁
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Agricultural University
Original Assignee
China Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China Agricultural University filed Critical China Agricultural University
Priority to CN202210732446.8A priority Critical patent/CN115287366A/en
Publication of CN115287366A publication Critical patent/CN115287366A/en
Priority to CN202310322848.5A priority patent/CN117286254A/en
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/30—Detection of binding sites or motifs
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a 15K SNP sequencing typing chip for breeding chicken with the day-old chicken laying initiation trait and application thereof. According to the invention, functional marker information related to the age of the chicken at birth is screened by utilizing genetic marker specificity obtained by a large-scale low-depth whole genome re-sequencing technology, noise sites in sequencing data information are greatly eliminated, and functional sites related to the age of the chicken at birth are retained, so that a 15K SNP sequencing typing chip applied to breeding of the chicken at birth age character is finally formed, the typing cost is reduced, and the breeding accuracy of the chicken at birth age character is greatly improved.

Description

15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application
Technical Field
The invention relates to the field of genome breeding, in particular to a 15K SNP sequencing typing chip for breeding chicken with the day-old chicken laying initiation trait and application thereof.
Background
In 2001, theo Meuwissen et al first proposed an innovative idea of whole Genome Selection (GS) technology, which is another innovative technology following implementation of BLUP Breeding technology in the last century, and it utilizes high-density markers covering whole genome to perform selective Breeding, and accelerates genetic progress by (1) shortening generation intervals through early Selection, (2) improving Breeding Value (GEBV) estimation accuracy, etc., especially having better prediction effect on complex traits of low heritability and difficult determination, and really using genome technology in Breeding practice, and is currently promoting revolutionary progress of animal plant Breeding, which is one of the most important and leading-edge common technologies in modern Breeding industry.
Single Nucleotide Polymorphisms (SNPs) are taken as the current mainstream genetic markers, and have the advantages of numerous numbers in genomes, wide distribution and good genetic stability. The method is widely applied to the research directions of analysis of various trait genetic mechanisms, selection evolution research, genome selection and the like in human and animal and plant research. In genome selection application, in the past decade, high-throughput SNP analysis mainly depends on an SNP chip technology, but the traditional solid-phase SNP chip has the defects of (1) incapability of expanding label fixation, (2) poor universality of different groups, (3) large difference of breeding effects of different phenotypes, high cost and the like, and the problems limit large-scale popularization and application of the whole genome selection technology in breeding. In recent years, sequencing-based SNP genotyping methods have been rapidly developed, and a typical method is a so-called "liquid-phase chip" method, which has the core principle of completing SNP genotyping of thousands to tens of thousands of sites by performing targeted enrichment sequencing on a genome target region. Although the technology is more flexible on design sites compared with the traditional solid phase chip, the analysis and use cost of the technology cannot be effectively reduced in principle due to the fact that the targeting performance of sequencing at different positions of a genome is greatly different, and large-scale breeding application is limited.
The open-producing age of chicken is an important economic trait, most resource groups of chicken, including local chicken species and commercial varieties, are moderate-intensity heritability (0.24-0.55) in the open-producing age, and the open-producing age of chicken is indicated to be genetically improved by breeding. Candidate gene analysis shows that reproductive axis genes such as growth hormone, prolactin, GDF-8 and the like are related to variation of the day age of laying-open, but research results still need to be further verified, and the whole genome analysis of the chicken at present shows that 9 chromosomes all carry the genes related to the day age of laying-open. That is, the trait still belongs to a typical quantitative trait, and the difficulty of directly and effectively applying the trait to the breeding industry through only a small number of molecular markers is large, and rapid breeding of the trait of the age of day of development still needs to be realized through a genome selection technology. With the advent of the genome era, the whole genome selection will provide powerful support for the analysis and application of the characters of the day of the poultry being produced.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a 15K SNP sequencing typing chip for breeding chicken with the day-old chicken laying initiation trait. According to the invention, functional marker information related to the chicken birth day age character is screened by utilizing genetic marker specificity obtained by a large-scale low-depth whole genome re-sequencing technology, noise sites in sequencing data information are greatly removed, and functional sites related to the chicken birth day age character are retained, so that a 15K SNP sequencing typing chip applied to breeding of the chicken birth day age character is finally formed, the typing cost is reduced, and meanwhile, the breeding accuracy of the chicken birth day age character is greatly improved.
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows:
A15K SNP sequencing and typing chip for breeding chicken laying day-old traits is characterized by comprising 14559 SNP loci.
The SNP sites in the sequencing and typing chip are shown in the following table:
Figure BDA0003713157010000031
Figure BDA0003713157010000041
Figure BDA0003713157010000051
Figure BDA0003713157010000061
Figure BDA0003713157010000071
Figure BDA0003713157010000081
Figure BDA0003713157010000091
Figure BDA0003713157010000101
Figure BDA0003713157010000111
Figure BDA0003713157010000121
Figure BDA0003713157010000131
Figure BDA0003713157010000141
Figure BDA0003713157010000151
Figure BDA0003713157010000161
Figure BDA0003713157010000171
the invention also aims to provide a manufacturing method of the 15K SNP sequencing and typing chip for breeding chicken laying day-old traits.
In order to achieve the purpose, the invention adopts the technical scheme that:
a manufacturing method of a 15K SNP sequencing typing chip for breeding chicken with the day-old chicken laid-open character is characterized by comprising the following steps:
step 1, performing low-depth resequencing on each individual genome DNA in a chicken sample population, wherein the sequencing depth is 0.4-0.8 x/sample;
step 2, carrying out genome SNP marker detection based on the sequencing data obtained in the step 1, identifying polymorphic sites in the chicken sample population, and carrying out genotyping on the polymorphic sites to obtain whole genome SNP;
step 3, taking the average age of the first egg laid by each individual in the chicken group as the age of the first egg laid by the chicken, recording the phenotype, and removing the outlier;
step 4, randomly selecting a fixed number of individuals from the chicken population with the outlier eliminated in the step 3 as a discovery population, carrying out whole genome association analysis on the chicken birth day age phenotype with the outlier eliminated in the step 3 based on the discovery population, and screening functional sites related to the chicken birth day age;
step 5, screening out genome skeleton sites for capturing other genome effects except for the main effect sites;
and 6, combining the functional sites related to the chicken day age at first birth obtained in the step 4 and the genome skeleton sites obtained in the step 5 to obtain 14559 marker sets specific to the chicken day age at first birth traits, and generating a final chicken day age at first birth trait breeding 15K SNP sequencing typing chip.
On the basis of the scheme: the site filtration parameters for identifying polymorphic sites in step 2 are as follows: estimating the minimum allele frequency EAF to be more than 0.01, and the sequencing depth to be more than or equal to 1.5IQR; in the step 2, the polymorphic loci are subjected to genotyping, and the filtering parameters of the genotyping result are as follows: minimum allele frequency MAF > 0.01, and filling information SCORE INFO SCORE > 0.4.
On the basis of the scheme: and 4, screening the functional sites related to the day-old of the chicken for first birth in the step 4, wherein the screening standard is that P is less than 0.0005.
On the basis of the scheme: the specific steps for screening out the genome framework loci in the step 5 are as follows: performing LD filtration on the whole genome SNP obtained in the step 2, wherein the filtration conditions are as follows: taking 1000bp as window length, LD r of two SNP sites in the window 2 And deleting one of the sites when the value is more than 0.25 to finally obtain the genome skeleton site.
The invention also aims to provide application of the 15K SNP sequencing and typing chip for breeding the chicken birth day-old traits.
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows:
the application of the 15K SNP sequencing typing chip for breeding the chicken laying day-old traits is characterized by comprising the following steps:
step 1, establishing a reference population, obtaining genotype data of each individual in the reference population by using the sequencing typing chip, simultaneously recording phenotype data of the reference population, and estimating an effect value of each SNP or different chromosome fragments through a proper statistical model;
step 2, carrying out genotyping on each individual of the candidate population by using the sequencing and typing chip method, and calculating the genome estimated breeding value of each individual in the candidate population by using the SNP effect value estimated in the reference population;
and 3, selecting and reserving the excellent individuals according to the ranking of the breeding values.
The invention relates to a chicken day-old-onset character breeding 15K SNP sequencing typing chip and application thereof, and has the beneficial effects that:
1. the chicken whole genome sequencing and typing chip disclosed by the invention does not depend on complex experimental procedures such as probe hybridization, targeted sequencing and the like of the traditional SNP chip, but identifies polymorphic sites and carries out genotyping by a low-depth sequencing technology, so that the cost of chip design is reduced, the cost of the chip applied to genome selective breeding is remarkably reduced, and the typing accuracy is up to more than 98%. The technology has low cost, high accuracy, convenience and rapidness, and can be rapidly popularized to the practice of genome selective breeding.
2. The chicken whole genome sequencing and typing chip provided by the invention also obviously increases the accuracy of breeding of the gene group of the birth day-old trait on the premise of reducing the number of marks of the traditional SNP chip, so that the genetic progress of selection of the gene group of the birth day-old trait is greatly accelerated, and the chip brings obvious economic benefits and has great value in chicken molecular breeding.
Drawings
The invention has the following drawings:
FIG. 1 is a genomic profile of age-related loci of the present invention.
Detailed Description
The present invention will be described in detail below by way of examples and accompanying tables. It is to be understood that the following examples are given for purposes of illustration only and that the description and accompanying tables are intended for purposes of clarity only and are not intended to limit the scope of the present invention. The features, operations, or characteristics described in the specification may be combined in any suitable manner in the various embodiments. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention.
Example 1: manufacturing method of 15K SNP sequencing typing chip for breeding chicken with day-old chicken production
Blood of 4527 chickens was collected to extract genomic DNA, and genomic library construction was performed. The genomic library of each sample was subjected to 0.5 x genome-wide resequencing. The polymorphic sites in the sample population were identified using BaseVar software with site filtration parameters: estimating the minimum allele frequency EAF to be more than 0.01 and the sequencing depth to be more than or equal to 1.5IQR; then, using STITCH software to perform genotyping on all polymorphic sites, wherein the filtering parameters of the genotyping result are as follows: the minimum allele frequency MAF > 0.01, and the filling information SCORE INFO SCORE > 0.4, finally resulting in the 8507838 SNP site.
Calculating the initial egg production age according to the average egg production age of each individual, recording the initial egg production age, and removing the outlier. Randomly selecting 1000 chickens from all samples as a site discovery group, carrying out whole genome association analysis on the production day-old phenotype based on the discovery group, and using a mixed linear model of GCTA, wherein covariates comprise batches and generations. And finally, 5982 important functional sites are screened according to the standard that P is less than 0.0005.
The genomic backbone sites were screened for capturing other genomic effects in addition to the major effect sites. Performing LD filtration on the whole genome SNP obtained by whole genome low-depth sequencing, wherein the filtration conditions are as follows: taking 1000bp as window length, LD r of two SNP sites in the window 2 When the value is more than 0.25, one of the sites is deleted, and finally 8581 total genomic framework sites are obtained.
Combining the open-producing day-age functional sites and the genome skeleton sites to obtain 14559 marker sets specific for the chicken open-producing day-age character, and generating a final chicken open-producing day-age character breeding 15K SNP sequencing typing chip.
Example 2: application of 15K SNP sequencing typing chip for breeding chicken birth day-old traits in genome selective breeding
To illustrate the effectiveness of the invention in obtaining the site set, two commercial SNP chips were used as controls, a kyoto chicken No. one commercial chip and an Illumina chicken 60K commercial chip, respectively.
The 4527 chickens in example 1 were divided into two groups, wherein 1000 individuals in example 1 were used as the discovery group and the remaining 3527 samples were used as the validation group, thereby avoiding information recycling and ensuring the robustness of the results. The 5X cross validation method is used for evaluating the performances of the specific marker set of the day age of laying hens and the two commercial chips, and the result shows that the prediction accuracy of the 15K SNP sequencing typing chip for breeding the chicken age of laying hens, invented by the invention, on the condition that the marker density is reduced by 2-4 times, is still higher, the accuracy is improved by 1.5% compared with the first Jing core and is improved by 3.0% compared with the 60K chip of Illumina chicken, and the effectiveness of the site set is illustrated.
TABLE 1 comparison of accuracy of 15K SNP sequencing typing chip for chicken breeding with day-old chicken birth traits and traditional chip
Figure BDA0003713157010000211
Those not described in detail in this specification are within the skill of the art.

Claims (6)

1. A15K SNP sequencing and typing chip for breeding chicken laying day-old traits is characterized by comprising 14559 SNP loci.
2. The manufacturing method of the 15K SNP sequencing typing chip for breeding chicken laying day-old traits as claimed in claim 1, characterized by comprising the following steps:
step 1, performing low-depth resequencing on each individual genome DNA in a chicken sample population, wherein the sequencing depth is 0.4-0.8 x/sample;
step 2, carrying out genome SNP marker detection based on the sequencing data obtained in the step 1, identifying polymorphic sites in the chicken sample group, and carrying out genotyping on the polymorphic sites to obtain whole genome SNP;
step 3, taking the average day age of the first egg laid by each individual in the chicken group as the day age of the first egg laid by the chicken, recording the phenotype, and removing the outlier;
step 4, randomly selecting a fixed number of individuals from the chicken population without the outlier in the step 3 as a discovery population, carrying out whole genome correlation analysis on the phenotype of the chicken birth date age without the outlier obtained in the step 3 based on the discovery population, and screening functional sites related to the chicken birth date age;
step 5, screening out the genome skeleton sites to capture other genome effects except the major effect sites;
and 6, combining the functional sites related to the chicken birth day age obtained in the step 4 and the genome skeleton sites obtained in the step 5 to obtain 14559 marker sets specific to the chicken birth day age character, and generating a final chicken birth day age character breeding 15K SNP sequencing typing chip.
3. The method for manufacturing a 15K SNP sequencing and typing chip for chicken birth day-old trait breeding according to claim 2, wherein the method comprises the following steps:
the site filtration parameters for identifying polymorphic sites in step 2 are as follows: estimating the minimum allele frequency EAF to be more than 0.01, and the sequencing depth to be more than or equal to 1.5IQR;
in the step 2, the polymorphic loci are subjected to genotyping, and the filtering parameters of the genotyping result are as follows: minimum allele frequency MAF > 0.01, and filling information SCORE INFO SCORE > 0.4.
4. The method for manufacturing a 15K SNP sequencing and typing chip for chicken birth day-old trait breeding according to claim 2, wherein the method comprises the following steps:
and 4, the screening standard for screening the functional sites related to the chicken birth day age trait in the step 4 is that P is less than 0.0005.
5. The method for manufacturing a 15K SNP sequencing and typing chip for chicken birth day-old trait breeding according to claim 2, wherein the method comprises the following steps:
the specific steps for screening out the genome framework loci in the step 5 are as follows: performing LD filtration on the whole genome SNP obtained in the step 2, wherein the filtration conditions are as follows: LD r of two SNP sites inside a window with 1000bp as the window length 2 And deleting one site when the value is more than 0.25 to finally obtain the genome skeleton site.
6. The application of the 15K SNP sequencing and typing chip for chicken birth day-old trait breeding according to claim 1 is characterized by comprising the following steps:
step 1, establishing a reference population, obtaining genotype data of each individual in the reference population by using the sequencing typing chip, simultaneously recording phenotype data of the reference population, and estimating an effect value of each SNP or different chromosome fragments through a proper statistical model;
step 2, carrying out genotyping on each individual of the candidate population by using the sequencing and typing chip method, and calculating the genome estimated breeding value of each individual in the candidate population by using the SNP effect value estimated in the reference population;
and 3, selecting and reserving the excellent individuals according to the ranking of the breeding values.
CN202210732446.8A 2022-06-24 2022-06-24 15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application Withdrawn CN115287366A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202210732446.8A CN115287366A (en) 2022-06-24 2022-06-24 15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application
CN202310322848.5A CN117286254A (en) 2022-06-24 2023-03-29 Chicken open birth day-old character breeding 15K SNP sequencing typing chip and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210732446.8A CN115287366A (en) 2022-06-24 2022-06-24 15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application

Publications (1)

Publication Number Publication Date
CN115287366A true CN115287366A (en) 2022-11-04

Family

ID=83820995

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202210732446.8A Withdrawn CN115287366A (en) 2022-06-24 2022-06-24 15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application
CN202310322848.5A Pending CN117286254A (en) 2022-06-24 2023-03-29 Chicken open birth day-old character breeding 15K SNP sequencing typing chip and application

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202310322848.5A Pending CN117286254A (en) 2022-06-24 2023-03-29 Chicken open birth day-old character breeding 15K SNP sequencing typing chip and application

Country Status (1)

Country Link
CN (2) CN115287366A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103276086A (en) * 2013-05-30 2013-09-04 扬州大学 Jinghai yellow chicken age-at-first-egg molecular genetic marker and application thereof
CN111235284A (en) * 2020-03-04 2020-06-05 河北工程大学 AMH gene SNP molecular marker related to laying age and egg yield of chicken and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103276086A (en) * 2013-05-30 2013-09-04 扬州大学 Jinghai yellow chicken age-at-first-egg molecular genetic marker and application thereof
CN111235284A (en) * 2020-03-04 2020-06-05 河北工程大学 AMH gene SNP molecular marker related to laying age and egg yield of chicken and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张德祥等: "土鸡开产日龄双向选择效果分析", 《中国家禽》 *

Also Published As

Publication number Publication date
CN117286254A (en) 2023-12-26

Similar Documents

Publication Publication Date Title
CN110191965B (en) Pig whole genome 50K SNP chip and application
US9976191B2 (en) Rice whole genome breeding chip and application thereof
CN113308562B (en) Cotton whole genome 40K single nucleotide site and application thereof in cotton genotyping
CN111088382B (en) Corn whole genome SNP chip and application thereof
CN115992265B (en) Grouper whole genome liquid phase chip and application thereof
CN116004898A (en) Peanut 40K liquid-phase SNP chip PeannitGBTS 40K and application thereof
CN118186103A (en) Lateolabrax japonicus 100k liquid phase chip and application thereof
CN118480613A (en) Pig whole genome low-density 5K SNP chip and application
CN115287366A (en) 15K SNP sequencing and typing chip for breeding chicken with open-laying day-old character and application
CN115287363A (en) 7K SNP sequencing typing chip for breeding chicken with weight traits of 91 days old and application
CN115287368A (en) 17K SNP sequencing typing chip for chicken carcass gravity breeding and application
CN115679011A (en) SNP molecular marker combination and application thereof in maize germplasm identification and breeding
CN115305288A (en) 80K SNP sequencing and typing chip for chicken slaughter rate sexual breeding and application
CN115287364A (en) 17K SNP sequencing typing chip for breeding up to hundred kilograms of weight and day-age characters of pigs and application
CN115287365A (en) Pig papillary number character breeding 130K SNP sequencing and typing chip and application
CN115747342A (en) Method for screening SNP (single nucleotide polymorphism) sites related to breeding of egg number characters of chickens and chip manufacturing method
CN115287367A (en) Pig backfat thickness character breeding 35K SNP sequencing and typing chip and application
CN112011629A (en) Jinfen white pig whole genome high-density SNP chip detection kit and application thereof
CN117025786B (en) Fine wool sheep 50K SNP liquid phase chip based on targeted capturing sequencing and application thereof
CN118086527B (en) Egg duck 20K low-density SNP chip based on targeted capture sequencing and application thereof
CN117305473B (en) SNP molecular marker of chicken immunity character related gene PLA2G7 and application thereof
CN118497352A (en) Oval pompano whole genome SNP molecular marker combination, liquid phase chip and application
CN118726559A (en) Biological breeding genotyping method and application
CN115011699A (en) Specific molecular identity card for identifying Nanyang black pig variety and application thereof
CN117334248A (en) Low-depth directional genome sequencing genotyping method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication

Application publication date: 20221104

WW01 Invention patent application withdrawn after publication